Device for interpreting data generated by nanopore sequencing

Abstract

The utility model discloses an interpretation device for nanopore sequencing generated data, and belongs to the technical field of nanopore sequencing. Comprising a nanometer sequencer body, the nanometer sequencer body is connected to a data interpretation module, the data interpretation module comprises an interpretation controller, an object selection unit, a storage unit, an interpretation display and a data updating unit, and the object selection unit comprises an interpretation object selection key and a selection determination key; the object selection unit is connected with the interpretation controller; the storage unit is connected to the interpretation controller, and an interpretation information database is arranged in the storage unit; the interpretation display is connected to the interpretation controller; and the data updating unit is connected to the interpretation controller. The device for interpreting the generated data of nanopore sequencing is simple in structure and high in applicability, and when the device is used, nanopore sequencing of a collected sample can be completed at a time, sequencing data can be generated, and the generated interpreted data can be quickly interpreted.

Description

Technical Field

[0001] The utility model specifically relates to a device for interpreting data generated by nanopore sequencing, and belongs to the technical field of nanopore sequencing. Background Art

[0002] In a nanopore sequencer, nanopores are distributed on a layer of high-resistivity polymer membrane; on both sides of the membrane are two chambers filled with electrolyte; because the nucleotide molecules themselves carry negative charges, they will flow from the cathode side to the anode side, thereby passing through the nanopore; when the DNA / RNA chain passes through the nanopore, the nanopore is also "reading" the chain; each nucleotide on the chain has a blocking effect on the ion current at the moment it occupies the nanopore; and because different nucleotides differ in size and spatial structure, the corresponding changes in ion current generated when passing through the nanopore are different; that is, each nucleotide has its own characteristic current change, like a unique fingerprint; therefore, real-time monitoring and recording of the current change data of a DNA / RNA chain passing through the nanopore can trace back and infer the nucleotide sequence. Most of the existing nanopore sequencing only completes the sequencing process, but cannot interpret the sequencing data. Utility Model Content

[0003] In order to solve the above problems, the utility model proposes a device for interpreting data generated by nanopore sequencing, which can perform nanopore sequencing, generate sequencing data, and quickly interpret the sequencing data.

[0004] The utility model is a device for generating data interpretation for nanopore sequencing, comprising a nanopore sequencer body, wherein the nanopore sequencer body is connected to a data interpretation module, wherein the data interpretation module comprises

[0005] Interpret the controller,

[0006] An object selection unit, the object selection unit comprising an interpretation object selection button and a selection confirmation button; the object selection unit is connected to the interpretation controller;

[0007] A storage unit, the storage unit is connected to the interpretation controller, and the storage unit has a built-in interpretation information database;

[0008] An interpretation display, wherein the interpretation display is connected to an interpretation controller;

[0009] A data updating unit is connected to the interpretation controller.

[0010] When in use, the object that needs to be sequenced and interpreted is selected through the object selection unit, and then the collected sample is sent to the nanosequencer body. After the nanosequencing is completed by the nanosequencer body and sequencing data is generated, the nanosequencer body then sends the sequencing data to the interpretation controller. The interpretation controller retrieves one or more interpretation data (calibrated nucleotide sequence) corresponding to the interpretation information database according to the interpretation object selected by the object selection unit, and matches the interpretation data with the sequencing data transmitted by the nanosequencer. After the matching is completed, the matching result is output to complete the sequencing generated data interpretation.

[0011] Furthermore, the input end of the data interpretation module is plug-connected with the nanosequencer body, and the data interpretation module and the nanosequencer body are separate structures. The data interpretation module serves as an auxiliary tool and is plug-matched with the nanosequencer body.

[0012] Furthermore, the data interpretation module is integrated inside the nanosequencer body; the interpretation display is the same display as the nanosequencer body, and the data interpretation module, as a functional unit of the nanosequencer body, is directly integrated inside the nanosequencer body.

[0013] Furthermore, the data interpretation module also includes a wireless transceiver unit, which is communicatively connected to a remote memory; a database update interface is provided on the remote memory; after the object selection unit obtains the selected object, the selected object is sent to the remote memory through the wireless transceiver unit, and the remote memory outputs one or more interpretation data according to the selected object, and sends the interpretation data to a storage unit of the data interpretation module, and uses the storage unit as a temporary storage unit.

[0014] Furthermore, the data updating unit is a data reading and writing interface or an online updating unit. When the database needs to be updated, the interpretation information database is updated through the data updating unit.

[0015] Compared with the prior art, the data interpretation device for nanopore sequencing generation of the utility model has a simple structure and strong applicability. When used, it can complete nanopore sequencing of collected samples at one time, generate sequencing data, and quickly interpret the generated interpretation data. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the connection structure between the nanosequencer body and the data interpretation module of the utility model.

[0017] Figure 2 It is a schematic diagram of the data interpretation module structure of the utility model.

[0018] Figure 3This is a schematic diagram of the communication connection between the data interpretation module and the remote storage of the utility model. DETAILED DESCRIPTION

[0019] Example:

[0020] like Figure 1 and Figure 2 The device for generating data interpretation for nanopore sequencing shown in the figure includes a nanosequencer body, the nanosequencer body is connected to a data interpretation module, and the data interpretation module includes

[0021] Interpret the controller,

[0022] An object selection unit, the object selection unit comprising an interpretation object selection button and a selection confirmation button; the object selection unit is connected to the interpretation controller;

[0023] A storage unit, the storage unit is connected to the interpretation controller, and the storage unit has a built-in interpretation information database;

[0024] An interpretation display, wherein the interpretation display is connected to an interpretation controller;

[0025] A data updating unit is connected to the interpretation controller.

[0026] When in use, the object that needs to be sequenced and interpreted is selected through the object selection unit, and then the collected sample is sent to the nanosequencer body. After the nanosequencing is completed by the nanosequencer body and sequencing data is generated, the nanosequencer body then sends the sequencing data to the interpretation controller. The interpretation controller retrieves one or more interpretation data (calibrated nucleotide sequence) corresponding to the interpretation information database according to the interpretation object selected by the object selection unit, and matches the interpretation data with the sequencing data transmitted by the nanosequencer. After the matching is completed, the matching result is output to complete the sequencing generated data interpretation.

[0027] For example, if the object selection unit selects trisomy 21, the interpretation controller obtains the calibrated nucleotide sequence corresponding to trisomy 21 from the storage unit, and the nanosequencer body sequences the blood containing fetal free DNA (cffDNA) in the collected sample to detect whether the fetus suffers from chromosomal aneuploidy. For example, it is used to obtain the calibrated nucleotide sequence of circulating tumor DNA and the calibrated nucleotide sequence of Ebola virus from the storage unit, etc.; it can realize on-site instant analysis.

[0028] The input end of the data interpretation module is plug-connected with the nanosequencer body. The data interpretation module and the nanosequencer body are separate structures. The data interpretation module serves as an auxiliary tool and is plug-matched with the nanosequencer body.

[0029] The data interpretation module is integrated inside the nanosequencer body; the interpretation display is the same display as the nanosequencer body, and the data interpretation module is directly integrated inside the nanosequencer body as a functional unit of the nanosequencer body.

[0030] like Figure 3 As shown, the data interpretation module also includes a wireless transceiver unit, which is communicatively connected to the remote memory; a database update interface is provided on the remote memory; after the object selection unit obtains the selected object, the selected object is sent to the remote memory through the wireless transceiver unit, and the remote memory outputs one or more interpretation data according to the selected object, and sends the interpretation data to the storage unit of the data interpretation module, and uses the storage unit as a temporary storage unit.

[0031] The data updating unit is a data reading and writing interface or an online updating unit. When the database needs to be updated, the interpretation information database is updated through the data updating unit.

[0032] The above embodiments are only preferred implementations of the present invention, so any equivalent changes or modifications made according to the structures, features and principles described in the scope of application of the present invention are included in the scope of application of the present invention.

Claims

1. A device for interpreting data generated by nanopore sequencing, comprising a nanopore sequencer body, characterized in that: The nanosequencer is connected to a data interpretation module, which includes Interpret the controller, An object selection unit, the object selection unit comprising an interpretation object selection button and a selection confirmation button; the object selection unit is connected to the interpretation controller; A storage unit, the storage unit is connected to the interpretation controller, and the storage unit has a built-in interpretation information database; An interpretation display, wherein the interpretation display is connected to an interpretation controller; A data updating unit is connected to the interpretation controller.

2. The device for interpreting data generated by nanopore sequencing according to claim 1, characterized in that: The data interpretation module input end is plug-connected to the nanosequencer body.

3. The device for interpreting data generated by nanopore sequencing according to claim 1, characterized in that: The data interpretation module is integrated inside the nanosequencer body; the interpretation display is the same display as the nanosequencer body.

4. The device for interpreting data generated by nanopore sequencing according to claim 1, characterized in that: The data interpretation module also includes a wireless transceiver unit, which is communicatively connected to a remote memory; a database update interface is provided on the remote memory.

5. The device for interpreting data generated by nanopore sequencing according to claim 1, characterized in that: The data updating unit is a data reading and writing interface or an online updating unit.

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