Organ-like culture device and dynamic fluid organ-like culture system

Abstract

The utility model relates to the field of bioengineering devices, and discloses an organoid culture device and a dynamic fluid organoid culture system. The organoid culture device comprises a row culture cavity and a support, the row culture cavity is installed on the support and comprises a plurality of culture units, the culture units are communicated through hollow channels, and a matrigel fixing ring is arranged at the bottom of the interior of each culture unit. According to the utility model, the dropping position and spreading degree of hydrogel can be limited through the matrigel fixing ring, and meanwhile, the protection effect on the hydrogel is achieved, and the standardization degree in organoid culture can be improved. According to the dynamic fluid organoid culture system provided by the utility model, the organoid culture device, the peristaltic pump and the liquid storage bottle are utilized to form circulation, so that the utilization rate of nutrient substances is favorably improved, and the reagent cost is reduced. Meanwhile, the existence of the liquid storage bottle can reduce the flow velocity fluctuation caused by the peristaltic pump, so that the flow velocity in the organoid culture device is more stable.

Description

Technical Field

[0001] The utility model relates to the field of bioengineering devices, and in particular to an organoid culture device and a dynamic fluid organoid culture system. Background Art

[0002] In vitro models are an important aid to human research on life science issues and have played a significant role in drug screening, disease simulation, life development and other fields. Organoids, as an emerging in vitro model, have a higher degree of simulation than traditional in vitro models, such as animal models and 2D cell models, and their cost is much lower than that of animal models. Therefore, they are widely popular and have replaced traditional in vitro models in many fields.

[0003] Stimulated by such high market demand, high-throughput culture of organoids has become the focus of attention. At present, high-throughput organoid culture on well plates is still the mainstream method. For example, using 48-well cell culture plates and 96-well cell culture plates can culture dozens or hundreds of organoids. Using organoid chips for high-throughput organoid culture is another method for high-throughput culture of organoids. Usually, such organoid chips can be composed of multiple microchambers, and the chambers are interconnected by flow channels. Whether it is a well plate or an organoid chip, it is necessary to manually use a pipette to add hydrogel to its chamber. When adding, it is hoped that the hydrogel is located in the same position of the chamber, for example, in the center of the chamber, to improve the degree of standardization and help characterize and analyze the organoid culture after completion. In particular, it is difficult to ensure that dozens of chambers can drip hydrogels at the same position during manual operation. In extreme cases, the hydrogel may even be dripped onto the side wall of the chamber. And because the physical and chemical properties of the surface of the well plate and the organoid chip material are not the same everywhere, the resulting subtle changes in wettability will cause the same volume of hydrogel droplets to spread differently, the height of the hydrogel droplets will change, and the morphology cannot be completely unified. Therefore, manual operation and the inherent defects of the material are currently a major difficulty in establishing standardized organoid culture operations. In addition, the hydrogel will degrade to a certain extent over time, and each time the culture medium is changed, the culture medium squeezed out of the pipette will cause a great impact on the hydrogel, thereby accelerating the degradation and destruction of the hydrogel, and the hydrogel will be displaced or damaged in terms of mobility during the culture process. Since the rigidity of the extracellular matrix required for organoid induction is certain, if the hydrogel is degraded too much, it will lead to failure of organoid induction, thereby reducing the activity of organoid culture and the induction success rate, resulting in increased culture costs.

[0004] In view of this, the present utility model is proposed. Utility Model Content

[0005] The utility model aims to provide an organoid culture device and a dynamic fluid organoid culture system.

[0006] The embodiment of the utility model is achieved as follows:

[0007] In a first aspect, the utility model provides an organoid culture device, which includes a row of culture chambers and a bracket, wherein the row of culture chambers is installed on the bracket, and the row of culture chambers includes a plurality of culture units, wherein the plurality of culture units are connected through a hollow channel, and a matrix glue fixing ring is provided at the inner bottom of each culture unit.

[0008] In an optional embodiment, the matrix gel fixing ring is a closed circular ring, a closed square ring or a closed star ring;

[0009] And / or, the matrix gel fixing ring includes at least one, and when there are multiple matrix gel fixing rings, they are arranged in an array.

[0010] In an optional embodiment, the matrix gel fixing ring is a closed circular ring, wherein the inner diameter of the matrix gel fixing ring is 1 mm-5 mm, the difference between the inner and outer diameters is 0.2 mm-0.7 mm, and the height is 0.5 mm-5 mm.

[0011] In an optional embodiment, there are multiple row culture chambers, and any two adjacent row culture chambers are connected in parallel or in series.

[0012] In an optional embodiment, any two adjacent row culture chambers are connected in series via a U-shaped tube, and the two open ends of the U-shaped tube are expansion joints.

[0013] In an optional embodiment, a liquid inlet pipe and a liquid outlet pipe are provided at both ends of the linked culture chamber, and the ends of the liquid inlet pipe and the liquid outlet pipe are contraction joints, and the contraction joints are cooperatively connected with the expansion joints.

[0014] In an optional embodiment, the number of the row culture chambers is 4-8, and the number of the culture units is 6-12.

[0015] In an optional embodiment, the bottom of any one of the culture units is circular, the top of the culture unit is elliptical, and the opening of the culture unit gradually increases from the bottom to the top.

[0016] In an optional embodiment, a mounting groove corresponding to the row culture chamber is provided on the bracket, the row culture chamber is provided in the mounting groove, a first buckle is provided on the outer side of the mounting groove, a transparent bottom plate is provided at the bottom of the row culture chamber, and second buckles cooperating with the first buckle are provided on both sides of the transparent bottom plate.

[0017] In an optional embodiment, the organoid culture device also includes a chamber cover, which is sealed and connected to the top of the row culture chamber, and the chamber cover includes a transparent top plate and a plurality of condensation rings, and the transparent top is used to cover the entire row culture chamber, and the plurality of condensation rings are all arranged on the transparent top plate and correspond one by one to the plurality of culture units.

[0018] In a second aspect, the utility model provides a dynamic fluid organoid culture system, which includes an organoid culture device as described in any one of the aforementioned embodiments, a peristaltic pump and a liquid storage bottle, wherein the liquid storage bottle is connected to the liquid inlet of the organoid culture device, the liquid outlet of the organoid culture device is connected to the peristaltic pump, and the peristaltic pump is connected to the liquid storage bottle.

[0019] The beneficial effects of the embodiments of the utility model are:

[0020] The organoid culture device provided by the utility model can realize the interconnection of multiple culture units through the arrangement of the linked culture chambers, wherein the matrix gel fixing ring arranged at the inner bottom of the culture unit can limit the movement of the gun head for dripping the hydrogel to limit the dripping position of the hydrogel, and at the same time limit the spreading degree of the hydrogel droplets on the surface, and at the same time play a protective role for the hydrogel, so as to ensure that the hydrogel gas-liquid interface area is maintained at a suitable position and a reasonable size, and the final shapes of the hydrogel droplets in the multiple culture units are also closer, which helps to improve the standardization degree in organoid culture.

[0021] The dynamic fluid organoid culture system provided by the utility model uses the organoid culture device, peristaltic pump and liquid storage bottle to form a cycle, so that nutrients are almost not wasted, less culture medium can support the full cycle culture of organoids, and reduce reagent costs. At the same time, the presence of the liquid storage bottle can reduce the flow rate fluctuation caused by the peristaltic pump, making the flow rate in the organoid culture device more stable. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the utility model and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying creative work.

[0023] Figure 1 A schematic diagram of the structure of an organoid culture device provided in the first embodiment of the utility model;

[0024] Figure 2 A front view of the row of culture chambers in the organoid culture device provided in the first embodiment of the utility model;

[0025] Figure 3 A top view of the row of culture chambers in the organoid culture device provided in the first embodiment of the utility model;

[0026] Figure 4 A side view of the row of culture chambers in the organoid culture device provided in the first embodiment of the utility model;

[0027] Figure 5 A schematic diagram of the structure of a U-shaped tube in an organoid culture device provided in the first embodiment of the utility model;

[0028] Figure 6 A top view of a support in an organoid culture device provided in the first embodiment of the utility model;

[0029] Figure 7 A side view of a support in an organoid culture device provided in the first embodiment of the utility model;

[0030] Figure 8 A front view of a chamber cover in an organoid culture device provided in the first embodiment of the utility model;

[0031] Fig. 9 A top view of a chamber cover in an organoid culture device provided in the first embodiment of the utility model;

[0032] Fig.10 This is a schematic structural diagram of the dynamic fluid organoid culture system provided in the second embodiment of the present invention.

[0033] Icons: 100-organoid culture device; 110-row culture chamber; 111-culture unit; 112-hollow channel; 113-matrix gel fixing ring; 114-liquid inlet tube; 115-liquid outlet tube; 1141-contraction joint; 116-U-shaped tube; 1161-expansion joint; 117-transparent bottom plate; 118-second buckle; 119-third buckle; 120-bracket; 121-mounting groove; 122-first buckle; 130-chamber cover; 131-transparent top plate; 132-condensation ring; 133-fourth buckle; 200-dynamic fluid organoid culture system; 210-peristaltic pump; 220-liquid storage bottle. DETAILED DESCRIPTION

[0034] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Generally, the components of the embodiments of the utility model described and shown in the drawings here can be arranged and designed in various different configurations.

[0035] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the present invention to be protected, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0036] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0037] In the description of the present utility model, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc. indicate the orientation or position relationship based on the orientation or position relationship shown in the accompanying drawings, or the orientation or position relationship in which the utility model product is usually placed when in use, which is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present utility model. In addition, the terms "first", "second", "third", etc. are only used to distinguish the description, and cannot be understood as indicating or implying relative importance.

[0038] In addition, the terms "horizontal", "vertical" and the like do not mean that the components are required to be absolutely horizontal or suspended, but can be slightly tilted. For example, "horizontal" only means that its direction is more horizontal than "vertical", and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0039] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0040] First embodiment

[0041] Please refer to Figure 1-Figure 9 The present embodiment provides an organoid culture device 100, which includes a row culture chamber 110 and a bracket 120. The row culture chamber 110 is installed on the bracket 120. The row culture chamber 110 includes a plurality of culture units 111. The plurality of culture units 111 are connected through a hollow channel 112. A matrix glue fixing ring 113 is provided at the inner bottom of each culture unit 111.

[0042] See also Figure 2 , Figure 3 and Figure 4 In this embodiment, the row culture chamber 110 refers to a plurality of culture units 111 arranged in parallel to form a row that is interconnected, wherein the number of the row culture chambers 110 can be one or more, for example, 4-8, and 6 are shown in the attached drawings, and the number of the culture units 111 is 6-10, and 8 are shown in the attached drawings. In addition, the center spacing of the 8 culture units 111 is consistent with the spacing of the pipette tips of the pipette row gun, which is convenient for batch operation.

[0043] That is, 6 groups of 8-row culture chambers 110 are placed on the bracket 120 at the same time, and the overall volume size is equivalent to a 96-well cell culture plate. Preferably, the number of row culture chambers 110 in this embodiment is multiple and any two adjacent row culture chambers 110 are connected in parallel or in series. That is, all of the multiple row culture chambers 110 can be connected in series, all in parallel, or partially in series and partially in parallel to meet the needs of culturing different organoids. For example, to culture 6 groups of 48 organs of the same type, 6 groups of 8-row chambers can be connected in series, and 1 culture medium storage bottle 220 can be used. If 6 different organoids are to be cultured, 8-row chambers can be selected in parallel, and 6 culture medium storage bottles 220 can be used respectively. If only 1 group of 8 organs of the same type need to be cultured, only 1 group of 8-row chambers and 1 culture medium storage bottle 220 need to be used.

[0044] The two ends of the row culture chamber 110 are provided with a liquid inlet pipe 114 and a liquid outlet pipe 115, and the ends of the liquid inlet pipe 114 and the liquid outlet pipe 115 are contraction joints 1141. The contraction joints 1141 are arranged to facilitate the subsequent cooperation with other joints when multiple row culture chambers 110 are connected in series or in parallel. Figure 1 and Figure 5 Any two adjacent row culture chambers 110 are connected in series through a U-shaped tube 116. The two open ends of the U-shaped tube 116 are expansion joints 1161. The contraction joint 1141 is connected with the expansion joint 1161 to facilitate connection and tightening, and avoid falling off and leakage.

[0045] In this embodiment, the bottom of any culture unit 111 in the row culture chamber 110 is circular, the top of the culture unit 111 is elliptical, and the opening of the culture unit 111 gradually increases from the bottom to the top. The design of small bottom and large top facilitates the operation of planting and taking out the gel.

[0046] In this embodiment, the multiple culture units 111 in the row culture chamber 110 are connected through the hollow channel 112. There is a certain height difference between the hollow channel 112 and the bottom of the culture unit 111. This setting can reduce the direct impact of the fluid on the glue droplets at the bottom of the culture unit 111.

[0047] In the present embodiment, the matrix gel fixing ring 113 is provided to limit the dripping position of the hydrogel and to limit it. The matrix gel fixing ring 113 is a closed circular ring, a closed square ring or a closed star-shaped ring. And / or, the matrix gel fixing ring 113 includes at least one, and when the matrix gel fixing ring 113 is multiple, it is arranged in an array. Specifically, the matrix gel fixing ring 113 is a closed circular ring, and the diameter of the matrix gel fixing ring 113 is smaller than the diameter of the culture unit 111 to fix the matrix gel, wherein the inner diameter size of the matrix gel fixing ring 113 is mainly to limit the movement of the gun head of the dripping hydrogel. If the inner diameter is too large, the problem of different dripping positions of the hydrogel cannot be solved. Therefore, preferably, the inner diameter of the matrix gel fixing ring 113 of the present application is 1mm-5mm, and the further preferred inner diameter is 4mm. The outermost boundary of the matrix gel fixing ring 113 can limit the spreading of the hydrogel drop on the surface. Preferably, the inner and outer diameter difference of the matrix gel fixing ring 113 is 0.2mm-0.7mm, and more preferably the inner and outer diameter difference is 0.5mm. The height of the matrix gel fixing ring 113 is used to limit the dripping position of the hydrogel and to ensure that the hydrogel gas-liquid interface area is maintained at a reasonable size. Therefore, the height of the matrix gel fixing ring 113 of the present application is 0.5mm-5mm, and preferably, the height is 1mm.

[0048] See also Figure 6 and Figure 7 In this embodiment, the bracket 120 is provided with a mounting groove 121 corresponding to the row culture chamber 110, the row culture chamber 110 is arranged in the mounting groove 121, the outer side of the mounting groove 121 is provided with a first buckle 122, the bottom of the row culture chamber 110 is provided with a transparent bottom plate 117, and both sides of the transparent bottom plate 117 are provided with second buckles 118 matched with the first buckle 122. The thickness of the transparent bottom plate 117 is less than 10 mm, preferably less than 2 mm, and the material of the transparent bottom plate 117 is a transparent material, including but not limited to at least one of glass, polymethyl methacrylate (PMMA), polypropylene (PP), polydimethylsiloxane (PDMS) and transparent resin.

[0049] Also, see Figure 8 and Fig. 9The organoid culture device 100 also includes a chamber cover 130, which is sealed and connected to the top of the row culture chamber 110. The chamber cover 130 includes a transparent top plate 131 and a plurality of condensation rings 132. The transparent top is used to cover the entire row culture chamber 110. The plurality of condensation rings 132 are all arranged on the transparent top plate 131 and correspond one to one with the plurality of culture units 111. The top of the row culture chamber 110 and the chamber cover 130 are also provided with snap-fit ​​structures, wherein the row culture chamber 110 is provided with a third snap-fit ​​119, and the chamber cover 130 is provided with a fourth snap-fit ​​133 at the position of the condensation ring 132. The third snap-fit ​​119 and the fourth snap-fit ​​133 cooperate to achieve the cooperative connection between the top of the row culture chamber 110 and the chamber cover 130. Through this structure, the chamber shell and the chamber cover 130 can be fastened and the sealing effect can be achieved. The setting of the condensation ring 132 can make the water evaporated during the culture process condense and fall after encountering the condensation ring 132, so that the problem of water evaporation in the culture medium is solved during the long-term organoid perfusion culture, and it can withstand a certain pressure change to prevent air leakage and liquid leakage. The thickness of the transparent top plate 131 is less than 5mm, preferably less than 2mm. The material of the transparent top plate 131 is a transparent material, including but not limited to at least one of glass, polymethyl methacrylate (PMMA), polypropylene (PP), polydimethylsiloxane (PDMS) and transparent resin.

[0050] The manufacturing methods of the components of the above-mentioned organoid culture device 100 include at least one of integral injection molding, mechanical milling and 3D printing.

[0051] Second embodiment

[0052] This example study found that the growth of organoids mainly depends on the support of multiple cytokines and the three-dimensional environment provided by the matrix gel. Although the organoid model has many advantages over the traditional model, it also has shortcomings. First, the organoids are cultured in well plates, and personnel need to regularly replace the culture medium. Once a human operating error occurs, the culture will fail. Second, it lacks the dynamic environment required by human organs and relies only on diffusion to transport nutrients and waste, which makes the organoids have different shapes and sizes, poor consistency, and is not conducive to variable control in related research. Third, it cannot meet the needs of co-culturing of multiple organoids.

[0053] In order to solve the above problems, some methods have been introduced to provide a better organoid culture method. For example, microfluidic technology is used to make a device containing multiple interconnected chambers, culture organoids in the chambers, connect to the drive pump through a tube, drive the culture medium to flow through the chambers, and reach the waste liquid chamber, providing a dynamic culture environment for the organoids. The multiple chambers interconnected on the device can also be used to culture different organoids to meet the needs of co-culture of multiple organoids. Although this method solves some pain points in the traditional organoid culture process, there are still some shortcomings. First, after the culture medium flows through the organoid, it enters the waste liquid chamber. Not only are the nutrients not fully utilized, but the culture medium needs to be replenished multiple times, and the cell secretions are also wasted. In particular, for the co-culture of multiple organoids, the interaction between the organoids cannot be reflected after the cell secretions are wasted, because the actual blood flow in the body is circulating, so when constructing an in vitro model, a circulating flow system is also required. Second, a stable flow rate is conducive to the growth of organoids, but commonly used drive pumps such as peristaltic pumps 210 and injection pumps have the problem of flow rate fluctuations due to the driving principle, which will cause problems in the growth of organoids.

[0054] To do this, see Fig.10 The present embodiment provides a dynamic fluid organoid culture system 200, which includes the organoid culture device 100, a peristaltic pump 210 and a liquid storage bottle 220 of the first embodiment described above, the liquid storage bottle 220 is connected to the liquid inlet of the organoid culture device 100, the liquid outlet of the organoid culture device 100 is connected to the peristaltic pump 210, and the peristaltic pump 210 is connected to the liquid storage bottle 220.

[0055] In this embodiment, the organoid culture device 100, the peristaltic pump 210 and the liquid storage bottle 220 are used to form a cycle. The peristaltic pump 210 drives the culture medium to circulate. The presence of the liquid storage bottle 220 can reduce the flow rate fluctuation caused by the peristaltic pump 210, making the flow rate in the organoid culture device 100 more stable. After the circulation flow is adopted, nutrients are almost not wasted, and less culture medium can support the full cycle culture of organoids, avoiding the problem of frequent replacement of culture medium in some devices or methods for culturing organoids, and reducing reagent costs. Among them, the structure of the peristaltic pump 210 and the liquid storage bottle 220 is a conventional structure, which is not described in detail in this embodiment.

[0056] According to different culture requirements, the organoid culture device 100 of the present application can flexibly select the number of 8-chamber groups and the series-parallel mode to be used to meet the needs. For example, if you want to culture 6 groups of 48 organs of the same type, you can connect 6 groups of 8-chambers in series and use 1 culture medium storage bottle 220. If you want to culture 6 different organoids, you can choose to connect 8 chambers in parallel and use 6 culture medium storage bottles 220 respectively. If you only need to culture 1 group of 8 organs of the same type, you only need to use 1 group of 8 chambers and 1 culture medium storage bottle 220. After determining the plan, start assembling the organoid culture device 100, and then connect the chip, storage bottle 220 and drive pump. Prepare the cell and matrix gel suspension processed by the standard process, add the matrix gel suspension to the matrix gel fixing ring 113 in the chip chamber, and then place the organoid culture device 100 in a 37°C environment to allow the matrix gel to fully solidify. After the matrix gel is completely solidified, an appropriate amount of organoid culture medium corresponding to the tissue type is added along the inner side wall of the chip chamber to reduce the time of filling the chamber after the medium circulation starts. The peristaltic pump 210 is started and finally driven to drive the medium circulation flow.

[0057] In summary, the organoid culture device 100 provided by the utility model can realize the interconnection of multiple culture units 111 through the setting of the row culture chamber 110, wherein the matrix gel fixing ring 113 provided at the bottom of the inner part of the culture unit 111 can limit the movement of the gun head of the hydrogel drop to limit the drop position of the hydrogel, and at the same time limit the spreading degree of the hydrogel drop on the surface, and at the same time play a protective role on the hydrogel, thereby ensuring that the hydrogel gas-liquid interface area is maintained at a suitable position and a reasonable size, and the final form of the hydrogel drop in the multiple culture units 111 is also closer, which helps to improve the standardization degree in organoid culture. The dynamic fluid organoid culture system 200 provided by the utility model uses the organoid culture device 100, the peristaltic pump 210 and the liquid storage bottle 220 to form a cycle, and nutrients are almost not wasted, and less culture medium can also support the full cycle culture of the organoid, reducing the reagent cost. At the same time, the presence of the liquid storage bottle 220 can reduce the flow rate fluctuation caused by the peristaltic pump 210, so that the flow rate in the organoid culture device 100 is more stable.

[0058] The above description is only the preferred embodiment of the utility model, and is not intended to limit the utility model. For those skilled in the art, the utility model can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. An organoid culture device, characterized in that: It includes a row culture chamber and a support, wherein the row culture chamber is installed on the support, and the row culture chamber includes a plurality of culture units arranged side by side, wherein the plurality of culture units are connected through a hollow channel, and a matrix glue fixing ring is arranged at the inner bottom of each culture unit.

2. The organoid culture device according to claim 1, characterized in that: The matrix gel fixing ring is a closed circular ring, a closed square ring or a closed star ring; And / or, the matrix gel fixing ring includes at least one, and when there are multiple matrix gel fixing rings, they are arranged in an array.

3. The organoid culture device according to claim 2, characterized in that: The matrix gel fixing ring is a closed circular ring, wherein the inner diameter of the matrix gel fixing ring is 1 mm-5 mm, the difference between the inner and outer diameters is 0.2 mm-0.7 mm, and the height is 0.5 mm-5 mm.

4. The organoid culture device according to claim 1, characterized in that: There are multiple row culture chambers, and any two adjacent row culture chambers are connected in parallel or in series. Any two adjacent row culture chambers are connected in series via a U-shaped tube, and the two open ends of the U-shaped tube are expansion joints.

5. The organoid culture device according to claim 4, characterized in that: A liquid inlet pipe and a liquid outlet pipe are arranged at both ends of the row culture chamber, and the ends of the liquid inlet pipe and the liquid outlet pipe are contraction joints, and the contraction joints are connected with the expansion joints.

6. The organoid culture device according to claim 4, characterized in that: The number of the row culture chambers is 4-8, and the number of the culture units is 6-12.

7. The organoid culture device according to claim 1, characterized in that: The bottom of any one of the culture units is circular, the top of the culture unit is elliptical, and the opening of the culture unit gradually increases from the bottom to the top.

8. The organoid culture device according to claim 1, characterized in that: The bracket is provided with an installation groove corresponding to the row culture chamber, the row culture chamber is arranged in the installation groove, the outer side of the installation groove is provided with a first buckle, the bottom of the row culture chamber is provided with a transparent bottom plate, and both sides of the transparent bottom plate are provided with second buckles that cooperate with the first buckle.

9. The organoid culture device according to claim 1, characterized in that: The organoid culture device also includes a chamber cover, which is sealed and connected to the top of the row culture chamber. The chamber cover includes a transparent top plate and multiple condensation rings. The transparent top is used to cover the entire row culture chamber. The multiple condensation rings are all arranged on the transparent top plate and correspond one by one to the multiple culture units.

10. A dynamic fluid organoid culture system, characterized in that: It comprises the organoid culture device according to any one of claims 1 to 9, a peristaltic pump and a liquid storage bottle, wherein the liquid storage bottle is connected to the liquid inlet of the organoid culture device, the liquid outlet of the organoid culture device is connected to the peristaltic pump, and the peristaltic pump is connected to the liquid storage bottle.

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