Salting-out device for separating animal serum protein

Abstract

The utility model relates to the technical field of salting-out, in particular to a salting-out device for separating animal serum protein, which comprises a first adjusting component, a second adjusting component, a neutral salt adding device, a buffer solution adding device, a generator, a dialysis bag and a pipetting cup, a generator is connected to the front face of the second adjusting assembly, a dialysis bag is connected to the lower end of the generator, a pipetting cup is arranged below the dialysis bag, a mounting plate is connected to the upper end of the second adjusting assembly, and a neutral salt adder and a buffer solution adder are connected to the mounting plate; the first adjusting assembly is composed of a base, a locking bolt, a first lifting air cylinder and a limiting column, the second adjusting assembly is composed of a mounting frame, a second lifting air cylinder, an electric linear sliding rail and a moving seat, through holes are formed in the two sides, close to the back face, of a mounting plate and the two sides, close to the back face, of the mounting frame, and a first control valve is arranged on the lower portion of the neutral salt adding device.

Description

Technical Field

[0001] The utility model relates to the technical field of salting out, in particular to a salting out device for separating animal serum proteins. Background Art

[0002] Manual addition of materials during salting out for separation of animal serum proteins has low stability, is prone to collision and contamination, and is inconvenient to separate proteins after salting out.

[0003] Therefore, it is necessary to design a salting-out device for separating animal serum proteins to solve the problems in the above-mentioned background technology. Utility Model Content

[0004] The utility model aims to solve the problems in the prior art and provides a salting-out device for separating animal serum proteins.

[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0006] A salting-out device for separating animal serum proteins, comprising a first regulating component, a second regulating component, a neutral salt adder, a buffer adder, a generator, a dialysis bag and a pipette cup, wherein the upper end of the first regulating component is connected to the second regulating component, the front of the second regulating component is connected to the generator, the lower end of the generator is connected to the dialysis bag, a pipette cup is provided below the dialysis bag, the upper end of the second regulating component is connected to a mounting plate, the mounting plate is connected to the neutral salt adder and the buffer adder;

[0007] The first adjustment assembly is composed of a base, a locking bolt, a first lifting cylinder and a limiting column, and the upper end of the base is connected to the locking bolt near the two sides of the back, and the first lifting cylinder and the limiting column are connected to the two sides of the upper end of the base;

[0008] The second adjustment assembly is composed of a mounting frame, a second lifting cylinder, an electric linear slide rail and a moving seat, and the second lifting cylinder is connected to both sides of the upper end of the mounting frame, the front of the mounting frame is connected to the electric linear slide rail, and the front of the electric linear slide rail is provided with a moving seat;

[0009] The mounting plate and the mounting frame are provided with through holes on both sides close to the back side;

[0010] A first control valve is provided at the lower part of the neutral salt adder, and a material distribution funnel is connected to the upper end of the neutral salt adder;

[0011] The lower part of the buffer additive is provided with a second control valve, the upper end of the buffer additive is connected with a liquid inlet pipe, and the liquid inlet pipe is provided with a third control valve;

[0012] A fourth control valve is provided at the lower part of the generator;

[0013] The outer portion of the upper portion of the dialysis bag is connected with a locking clamp;

[0014] The lower end of the pipetting cup is connected with a supporting frame.

[0015] As a preferred solution of the utility model, the base is fixed to the operating table by locking bolts, the first lifting cylinder and the base and the mounting frame are connected by bolts, and the limit column and the mounting frame and the through-holes on the mounting plate are connected by socket connection.

[0016] As a preferred solution of the utility model, the second lifting cylinder and the mounting frame and the mounting plate are connected by bolts, and the mounting frame and the electric linear slide rail are connected by bolts.

[0017] As a preferred solution of the utility model, the generator and the movable seat are connected by plugging, and the upper part of the dialysis bag is sleeved on the outside of the generator and locked by a locking clamp.

[0018] As a preferred solution of the utility model, the neutral salt adder, the buffer adder and the mounting plate are connected by plugging.

[0019] As a preferred solution of the utility model, the liquid inlet pipe and the sealing plug at the upper end of the buffer additive are plug-connected.

[0020] As a preferred solution of the utility model, the sealing plug at the upper end of the dividing funnel and the neutral salt adder is plug-connected.

[0021] In summary, the technical effects and advantages of the utility model are as follows: when the salting-out device for separating animal serum protein is used, neutral salt is added to the material dividing funnel, and then the dosage is controlled by the first control valve at the bottom of the neutral salt adder, and the neutral salt is added to the generator. When the neutral salt is added to the protein solution, the high-concentration salt ions have a strong hydration force, which can seize the hydration layer of the protein molecules and make them "dehydrated", so that the protein colloid particles coagulate and precipitate out. When in use, the neutral salt adder is driven up and down by the second lifting cylinder, so that the neutral salt adder is convenient to insert into and separate from the generator, and the position is relatively fixed, which is better than manual operation. The feeding is more stable. After the protein is separated by salting out precipitation, the salt in the protein needs to be removed. The protein solution enters the dialysis bag under the control of the fourth control valve at the bottom of the generator, and the buffer enters the buffer adder through the liquid inlet pipe. The buffer is controlled by the second control valve at the bottom of the buffer adder for dialysis, and the buffer is continuously replaced to complete the dialysis. The generator can move along the length direction of the electric linear slide rail to move to the corresponding position below the neutral salt adder and the buffer adder, and the dialysis bag can be driven up and down by the first lifting cylinder to facilitate the dialysis bag to be placed in and taken out of the pipette cup. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is the overall structure diagram of the utility model;

[0023] Figure 2 This is a structural diagram of the neutral salt adder of the utility model;

[0024] Figure 3 This is a structural diagram of the second adjustment component of the utility model;

[0025] Figure 4 This is a structural diagram of a dialysis bag of the utility model;

[0026] Figure 5 This is a structural diagram of the first adjustment component of the utility model;

[0027] Figure 6 This is a structural diagram of the pipette cup of the present utility model.

[0028] In the figure: 1. first adjusting component; 101. base; 102. locking bolt; 103. first lifting cylinder; 104. limiting column; 2. second adjusting component; 201. mounting frame; 202. second lifting cylinder; 203. electric linear slide; 204. moving seat; 3. mounting plate; 301. through hole; 4. neutral salt adder; 401. first control valve; 402. dividing funnel; 5. buffer adder; 501. second control valve; 502. third control valve; 503. liquid inlet pipe; 6. generator; 601. fourth control valve; 7. dialysis bag; 701. locking clamp; 8. pipette cup; 801. support frame. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0030] Reference Figure 1-Figure 6, a salting-out device for separating animal serum proteins, comprising a first regulating component 1, a second regulating component 2, a neutral salt adder 4, a buffer adder 5, a generator 6, a dialysis bag 7 and a pipette cup 8, wherein the upper end of the first regulating component 1 is connected to the second regulating component 2, the front of the second regulating component 2 is connected to the generator 6, the lower end of the generator 6 is connected to the dialysis bag 7, a pipette cup 8 is arranged below the dialysis bag 7, the upper end of the second regulating component 2 is connected to a mounting plate 3, the mounting plate 3 is connected to the neutral salt adder 4 and the buffer adder 5; the first regulating component 1 is composed of a base 101, a locking bolt 102, a first lifting cylinder 103 and a limiting column 104, and the upper end of the base 101 is connected to the locking bolts 102 near the back side, and the upper end of the base 101 is connected to the first lifting cylinder 103 and the limiting column 104; the second regulating component 2 is composed of a mounting plate 3, a locking bolt 102, a first lifting cylinder 103 and a limiting column 104, and the locking bolts 102 are connected to the locking bolts 102 at both sides of the upper end of the base 101 The mounting frame 201 is composed of a second lifting cylinder 202, an electric linear slide 203 and a moving seat 204, and the second lifting cylinder 202 is connected to both sides of the upper end of the mounting frame 201, the electric linear slide 203 is connected to the front of the mounting frame 201, and a moving seat 204 is provided at the front of the electric linear slide 203; through holes 301 are opened on both sides of the mounting plate 3 and the mounting frame 201 near the back; a first control valve 401 is provided at the lower part of the neutral salt adder 4, and a dividing funnel 402 is connected to the upper end of the neutral salt adder 4; a second control valve 501 is provided at the lower part of the buffer adder 5, and a liquid inlet pipe 503 is connected to the upper end of the buffer adder 5, and a third control valve 502 is provided on the liquid inlet pipe 503; a fourth control valve 601 is provided at the lower part of the generator 6; a locking clamp 701 is connected to the outside of the upper part of the dialysis bag 7; and a support frame 801 is connected to the lower end of the pipette cup 8.

[0031] Reference Figure 1 , Figure 2 , Figure 3 and Figure 5, manual feeding stability is low during salting out for animal serum protein separation, and it is easy to collide and cause contamination, and it is inconvenient to separate proteins after salting out. The base 101 is fixed to the operating table by a locking bolt 102, and the first lifting cylinder 103 and the base 101 and the mounting frame 201 are connected by bolts. The limit column 104 and the mounting frame 201 and the through hole 301 on the mounting plate 3 are sleeve-connected, and the second lifting cylinder 202 and the mounting frame 201 and the mounting plate 3 are connected by bolts. The mounting frame 201 and the electric linear slide rail 203 are connected by bolts. The neutral salt adder 4, the buffer adder 5 and the mounting plate 3 are connected by plug-in connection, and the material distribution funnel 4 02 is plugged into the sealing plug at the upper end of the neutral salt adder 4. When in use, the neutral salt is added to the distribution funnel 402, and then the dosage is controlled by the first control valve 401 at the lower part of the neutral salt adder 4, and the neutral salt is added to the generator 6. The neutral salt is added to the protein solution. The high concentration of salt ions has a strong hydration power, which can seize the hydration layer of the protein molecules and make them "dehydrated", so that the protein colloid particles coagulate and precipitate. When in use, the neutral salt adder 4 is driven up and down by the second lifting cylinder 202, so as to facilitate the insertion of the neutral salt adder 4 into the generator 6 and the separation from the generator 6. The position is relatively fixed, which is more stable than manual feeding.

[0032] Reference Figure 1-Figure 6 The generator 6 and the movable seat 204 are connected by plugging, the upper part of the dialysis bag 7 is sleeved on the outside of the generator 6 and locked by the locking clamp 701, the liquid inlet pipe 503 and the sealing plug at the upper end of the buffer additive 5 are plugged and connected, after the protein is separated by salting out precipitation, the salt in the protein needs to be removed, the protein solution enters the dialysis bag under the control of the fourth control valve 601 at the bottom of the generator 6, the buffer enters the buffer additive 5 through the liquid inlet pipe 503, the buffer is controlled by the second control valve 501 at the bottom of the buffer additive 5 to perform dialysis, and the buffer is continuously replaced to complete the dialysis, the generator 6 can move along the length direction of the electric linear slide 203, so as to move to the corresponding position below the neutral salt additive 4 and the buffer additive 5, and the dialysis bag 7 can be driven up and down by the first lifting cylinder 103, so as to facilitate the dialysis bag 7 to be placed in and taken out of the pipette cup 8.

[0033] Working principle: The base 101 of the salting-out device for separating animal serum protein is fixed on the operating table by a locking bolt 102, the first lifting cylinder 103 and the base 101 and the mounting frame 201 are connected by bolts, the limit column 104 and the mounting frame 201 and the through hole 301 on the mounting plate 3 are sleeve-connected, the second lifting cylinder 202 and the mounting frame 201 and the mounting plate 3 are connected by bolts, the mounting frame 201 and the electric linear guide rail 203 are connected by bolts, the neutral salt adder 4, the buffer adder 5 and the mounting plate 3 are connected by plugging, and the sealing plug at the upper end of the distribution funnel 402 and the neutral salt adder 4 is plugged and connected. When in use, the neutral salt is added to the distribution funnel 402, and then the amount is controlled by the first control valve 401 at the lower part of the neutral salt adder 4, and the neutral salt is added to the generator 6. The neutral salt is added to the protein solution. The high concentration of salt ions has a strong hydration force, which can seize the hydration layer of the protein molecules and make them "dehydrated", so that the protein colloid particles condense and precipitate. When in use, the neutral salt adder is driven by the second lifting cylinder 202. The adder 4 moves up and down, so that the neutral salt adder 4 is conveniently inserted into and separated from the generator 6. The position is relatively fixed, which is more stable than manual feeding. The generator 6 and the movable seat 204 are connected by plugging. The upper part of the dialysis bag 7 is sleeved on the outside of the generator 6 and is locked by the locking clamp 701. The liquid inlet pipe 503 and the sealing plug at the upper end of the buffer adder 5 are plugged and connected. After the protein is separated by salting out precipitation, the salt in the protein needs to be removed. The protein solution is controlled by the fourth control valve 601 at the bottom of the generator 6. The buffer solution enters the dialysis bag through the liquid inlet pipe 503, and the buffer solution is controlled by the second control valve 501 at the bottom of the buffer solution adder 5 to perform dialysis, and the buffer solution is continuously replaced to complete the dialysis. The generator 6 can move along the length direction of the electric linear slide 203, so as to move to the corresponding position below the neutral salt adder 4 and the buffer solution adder 5, and the dialysis bag 7 can be driven by the first lifting cylinder 103 to move up and down, so as to facilitate the dialysis bag 7 to be placed in and taken out of the pipette cup 8.

[0034] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A salting-out device for separating animal serum proteins, comprising a first regulating component (1), a second regulating component (2), a neutral salt adder (4), a buffer adder (5), a generator (6), a dialysis bag (7) and a pipetting cup (8), characterized in that: The upper end of the first regulating component (1) is connected to the second regulating component (2), the front of the second regulating component (2) is connected to a generator (6), the lower end of the generator (6) is connected to a dialysis bag (7), a pipette cup (8) is provided below the dialysis bag (7), the upper end of the second regulating component (2) is connected to a mounting plate (3), and the mounting plate (3) is connected to a neutral salt adder (4) and a buffer adder (5); The first adjustment component (1) is composed of a base (101), a locking bolt (102), a first lifting cylinder (103) and a limiting column (104), and the locking bolt (102) is connected to both sides of the upper end of the base (101) near the back, and the first lifting cylinder (103) and the limiting column (104) are connected to both sides of the upper end of the base (101); The second adjustment component (2) is composed of a mounting frame (201), a second lifting cylinder (202), an electric linear slide rail (203) and a moving seat (204), and the second lifting cylinder (202) is connected to both sides of the upper end of the mounting frame (201), the electric linear slide rail (203) is connected to the front of the mounting frame (201), and the moving seat (204) is provided at the front of the electric linear slide rail (203); The mounting plate (3) and the mounting frame (201) are both provided with through holes (301) on both sides close to the back side; The lower part of the neutral salt adder (4) is provided with a first control valve (401), and the upper end of the neutral salt adder (4) is connected with a material distribution funnel (402); A second control valve (501) is provided at the lower part of the buffer additive (5), a liquid inlet pipe (503) is connected to the upper end of the buffer additive (5), and a third control valve (502) is provided on the liquid inlet pipe (503); A fourth control valve (601) is provided at the lower part of the generator (6); The outer portion of the upper portion of the dialysis bag (7) is connected to a locking clamp (701); The lower end of the pipetting cup (8) is connected to a support frame (801).

2. The salting-out device for separating animal serum proteins according to claim 1, characterized in that: The base (101) is fixed on the operating table by means of locking bolts (102); the first lifting cylinder (103) and the base (101) and the mounting frame (201) are connected by bolts; the limiting column (104) and the mounting frame (201) and the through hole (301) on the mounting plate (3) are sleeve-connected.

3. The salting-out device for separating animal serum proteins according to claim 1, characterized in that: The second lifting cylinder (202) and the mounting frame (201) and the mounting plate (3) are connected via bolts, and the mounting frame (201) and the electric linear slide rail (203) are connected via bolts.

4. The salting-out device for separating animal serum proteins according to claim 1, characterized in that: The generator (6) and the movable seat (204) are connected by plugging, and the upper part of the dialysis bag (7) is sleeved on the outside of the generator (6) and is locked in position by a locking clamp (701).

5. The salting-out device for separating animal serum proteins according to claim 1, characterized in that: The neutral salt adder (4), the buffer adder (5) and the mounting plate (3) are connected by plugging.

6. The salting-out device for separating animal serum proteins according to claim 1, characterized in that: The liquid inlet pipe (503) and the sealing plug at the upper end of the buffer additive (5) are plug-connected.

7. The salting-out device for separating animal serum proteins according to claim 1, characterized in that: The sealing plug at the upper end of the distribution funnel (402) and the neutral salt additive (4) is plug-connected.

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