Nucleic acid aerosol pollution collecting device

Abstract

The utility model relates to the field of pathogen nucleic acid detection, in particular to a nucleic acid aerosol pollution collecting device, which is characterized in that under the action of an exhaust fan, a horn mouth is used as an air inlet, air containing nucleic acid pollution flows through a collecting film, aerosol is captured by the collecting film, and the air passes through a gap between a base plate at the top end of a shell and the shell or a through hole of the base plate; the air flows to the air outlet. Under the continuous action of the exhaust fan, the process can be circulated all the time, so that the collecting film has more opportunities to collect possible aerosol. And taking down the collection membrane, eluting the collected nucleic acid with normal saline, carrying out high-sensitivity fluorescent quantitative PCR, and if the amplification result is positive, determining that the nucleic acid aerosol pollution of the pathogen exists, otherwise, determining that the nucleic acid aerosol pollution of the pathogen does not exist.

Description

Technical Field

[0001] The utility model relates to the field of pathogen nucleic acid detection, in particular to a collection device for nucleic acid aerosol pollution. Background Art

[0002] Nucleic acid detection technology plays an extremely important role in pathogen detection. Among them, PCR technology, especially fluorescent quantitative PCR technology, can quickly and accurately detect extremely small amounts of pathogen nucleic acid fragments because of its short amplification fragments, fast amplification speed, and extremely high sensitivity. Therefore, trace nucleic acid fragment contamination in the nucleic acid detection room will also result in false positive results. In the process of nucleic acid detection, due to the friction between the flowing air and the liquid surface during the opening of the tube cover or pipetting, high concentrations of pathogen nucleic acids or plasmids or PCR products will produce aerosol contamination, resulting in misjudgment of the positive and negative nature of the pathogen.

[0003] Before testing the pathogen, it is necessary to determine whether the test room is contaminated by the nucleic acid aerosol of the pathogen. At present, the common operation in the laboratory is to add physiological saline to several centrifuge tubes, place them open in different locations of the PCR laboratory for several hours, and then perform fluorescent quantitative PCR amplification on the physiological saline. If the amplification result is positive, there is nucleic acid aerosol contamination of the pathogen, otherwise, there is no nucleic acid aerosol contamination. In this way, the direction of air flow is uncontrollable, and the orifice area of ​​the centrifuge tube is small, and the contact area with the air flow is limited, so it is difficult to collect enough nucleic acid aerosol. If the amount of aerosol collected is lower than the detection limit, the presence of aerosol cannot be detected, which makes the tester mistakenly believe that there is no aerosol contamination in the laboratory, resulting in a false positive result of the pathogen test. Therefore, an efficient nucleic acid aerosol contamination collection device is needed to ensure the accuracy of the results. Utility Model Content

[0004] Aiming at the disadvantage that the above-mentioned method of monitoring aerosol contamination by adding physiological saline to a centrifuge tube is prone to missed detection, the purpose of the utility model is to provide a collection device for nucleic acid aerosol contamination to solve the above-mentioned technical problem.

[0005] The purpose of the utility model can be achieved through the following technical solutions:

[0006] A collection device for nucleic acid aerosol pollution comprises a shell, an exhaust fan is installed in the shell, a bracket is arranged around the edge of the shell at the top opening of the shell, a pad can be installed on the bracket, and a ventilated gap is left between the pad and the shell; a collection membrane is placed on the pad, and the collection membrane is used to collect nucleic acid aerosol.

[0007] An air inlet duct is installed on the top of the shell, and the air inlet duct is designed to be trumpet-shaped.

[0008] The shell is a cylindrical cavity with two open ends. The open top end of the shell is connected to the air inlet duct; the open bottom end of the shell is used for air outlet.

[0009] A strip bracket is installed inside the shell, and the exhaust fan is installed on the strip bracket.

[0010] There is a gap between the pad and the top opening of the shell body.

[0011] The pad is provided with a through hole.

[0012] The pad can be removed freely and can be reused after being cleaned, disinfected and dried.

[0013] The collecting film is made of flocking, paper or cotton.

[0014] The above technical solution is adopted, and under the action of the exhaust fan, the trumpet-shaped air inlet duct is used as the air inlet, and the air flow driven by the wind passes through the collection membrane, so that the nucleic acid aerosol is captured by the collection membrane. The diameter of the collection membrane and the pad are both smaller than the diameter of the shell, so that the air can flow to the air outlet through the pores between the pad and the shell or the through holes of the pad. Under the continuous action of the exhaust fan, this process can be circulated all the time, so that the collection membrane has more opportunities to collect possible aerosols. The collection membrane is removed, and the nucleic acid collected on the collection membrane is eluted with physiological saline, and a high-sensitivity fluorescent quantitative PCR is performed. If the amplification result is positive, there is nucleic acid aerosol contamination of the pathogen, otherwise, there is no nucleic acid aerosol contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The utility model is further described below in conjunction with the accompanying drawings.

[0016] Figure 1 It is a cross-sectional view of the collecting device of the present utility model.

[0017] Figure 2 It is an overall schematic diagram of the collecting device of the utility model.

[0018] 1. Shell; 2. Air inlet duct; 3. Exhaust fan; 4. Pad; 5. Bracket; 6. Air outlet; 7. Strip bracket. DETAILED DESCRIPTION

[0019] The specific implementation methods of the present invention are further described below in conjunction with the accompanying drawings. It should be noted that the description of these implementation methods is used to help understand the present invention, but does not constitute a limitation of the present invention. In addition, the technical features involved in each implementation method of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0020] It should be noted that, in the description of the present invention, the directions or positional relationships indicated by the terms "upper", "lower", "left", "right", "front", "back", etc. are descriptions of the structure of the present invention based on the accompanying drawings, and are only for the convenience of describing the present invention, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on the present invention.

[0021] Please refer to the attached Figure 1-2 As shown, the utility model discloses a collection device for nucleic acid aerosol pollution, comprising a housing 1, an exhaust fan 3 is installed in the housing 1, and a bracket 5 is provided around the inner edge of the housing 1 at the top opening of the housing 1. A pad 4 can be installed on the bracket 5; a collection membrane is placed on the pad 4, and the collection membrane is used to collect nucleic acid aerosols; the diameters of the collection membrane and the pad 4 are both smaller than the housing 1, so that air can flow to the air outlet through the gap between the pad 4 and the housing 1 or the through hole of the pad 4, and the pad 4 can be freely removed and reused after cleaning, disinfection and drying.

[0022] An air inlet duct 2 is installed on the top of the shell 1. The air inlet duct 2 is designed to be trumpet-shaped so that more air flows to the collection membrane to fully collect the aerosol.

[0023] The shell 1 is a cylindrical cavity with two open ends. The open top end of the shell 1 is connected to the air inlet duct 2; the open bottom end of the shell 1 is used for air outlet.

[0024] A strip bracket 7 is installed inside the housing 1 , and the exhaust fan 3 is installed on the strip bracket 7 .

[0025] There is a gap between the pad 4 and the top opening of the shell 1 .

[0026] The pad 4 is provided with through holes, and the through holes on the pad 4 facilitate the air to continue to flow downward after passing through the collection membrane.

[0027] The collecting film is made of flocking, paper or cotton.

[0028] The method for collecting and detecting nucleic acid aerosols using the above device is as follows: close the PCR laboratory, place the sterile pad 4 on the bracket 5, place the sterile collection membrane on the pad 4, connect the utility model to the power supply, turn on the switch, adjust the required wind speed and time on the control panel, start the device, and under the action of the exhaust fan 3, the air in the room enters at the air inlet and hits the collection membrane. If there is nucleic acid aerosol in the room, the aerosol will be captured by the collection membrane, and the air flow will flow downward from the pores between the bracket 5 and the shell 1 around the bottom of the speaker or the through holes of the pad 4 to the air outlet 6 for discharge. In the closed room, the air flows repeatedly, so that the collection membrane collects the aerosol many times. Finally, the collection membrane is removed, the membrane surface is repeatedly washed with physiological saline, and then the physiological saline is taken to perform fluorescent quantitative PCR of the pathogen to be detected, and the presence or absence of aerosol contamination of the pathogen is determined based on the results; the pad 4 can be reused after being cleaned and dried with a nucleic acid remover.

[0029] The embodiments of the present invention are described in detail above with reference to the accompanying drawings, but the present invention is not limited to the described embodiments. For those skilled in the art, various changes, modifications, substitutions and variations of these embodiments are made without departing from the principles and spirit of the present invention, and still fall within the scope of protection of the present invention.

Claims

1. A collection device for nucleic acid aerosol contamination, characterized in that: The invention comprises a shell (1), wherein an exhaust fan (3) is installed in the shell (1), and a bracket (5) is provided around the inner edge of the shell (1) at the top opening of the shell (1), and a pad (4) can be installed on the bracket (5); a collection membrane is placed on the pad (4), and the collection membrane is used to collect nucleic acid aerosols; An air inlet duct (2) is installed on the top of the shell (1), and the air inlet duct (2) is designed to be trumpet-shaped.

2. A nucleic acid aerosol contamination collection device according to claim 1, characterized in that: The shell (1) is a cylindrical hollow body with two open ends; the open top end of the shell (1) is connected to the air inlet duct (2); the open bottom end of the shell (1) is an air outlet (6).

3. A nucleic acid aerosol contamination collection device according to claim 1, characterized in that: A strip-shaped bracket (7) is installed inside the housing (1), and the exhaust fan (3) is installed on the strip-shaped bracket (7).

4. A nucleic acid aerosol contamination collection device according to claim 1, characterized in that: There is a gap between the pad (4) and the top opening of the shell (1).

5. A nucleic acid aerosol contamination collection device according to claim 1, characterized in that: The pad (4) is provided with a through hole.

6. A nucleic acid aerosol contamination collection device according to claim 1, characterized in that: The collecting membrane is a flocking material.

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