Fermentation tank beneficial to improvement of halophilic strain density

Abstract

The utility model provides a fermentation tank beneficial to improving the density of halophilic strains, belongs to the technical field of halophilic strain fermentation, and aims to solve the problems that the inner wall of the conventional fermentation tank is relatively smooth and the halophilic strains are inconvenient to adhere. Comprising a tank body; a control module is fixedly arranged at the top of the tank cover; a ventilation structure is fixedly arranged between the outer side of the tank body and the interior of the tank cover, a stirring assembly is rotationally arranged in the bottom side of the tank body, and an automatic pressure relief structure is fixedly arranged in the top side of the tank cover; by means of the annular array type attachment protrusions and the attachment grooves, a microstructure can be formed on the inner wall of the tank body, and attachment and growth of halophilic bacteria are facilitated; meanwhile, a ventilation structure and an oxygen sensor are utilized, so that sufficient supply of oxygen in the culture solution can be ensured; a fermentation environment capable of improving the density of halophilic bacteria is provided.

Description

Technical Field

[0001] The utility model belongs to the technical field of halophilic bacteria fermentation, and in particular relates to a fermentation tank which is beneficial to improving the density of halophilic bacteria. Background Art

[0002] Halophiles can grow and reproduce in extreme saline environments; Halophiles can survive and degrade organic matter in high-salt environments, so halophiles can purify high-salt wastewater, and halophilic bacteria usually require fermentation tanks for fermentation and cultivation.

[0003] When fermenting and culturing halophilic bacteria, existing halophilic bacteria fermentation tanks usually use fixed electric heating rods to heat the halophilic bacteria culture solution. The temperature of the culture solution far away from the electric heating rod may not reach the reproduction temperature of the halophilic bacteria, thereby reducing the reproduction rate of the halophilic bacteria. In addition, the inner wall of the existing fermentation tank is relatively smooth, which is not convenient for halophilic bacteria to attach, resulting in a low density of halophilic bacteria and reducing the practicality of the fermentation tank. Utility Model Content

[0004] In order to solve the above technical problems, the utility model provides a fermentation tank which is beneficial to increasing the density of halophilic bacteria, is beneficial to the attachment and growth of halophilic bacteria, can ensure sufficient oxygen supply in the culture solution and the fermentation temperature and reproduction rate of halophilic bacteria, and provides a fermentation environment that can increase the density of halophilic bacteria.

[0005] The utility model provides a fermentation tank which is beneficial to improving the density of halophilic bacteria, comprising: a tank body; support legs are fixedly arranged at the bottom of the tank body in a circular array, and a tank cover is fixedly installed on the top of the tank body; the tank cover and the tank body are both made of special alloy material, and the tank cover and the tank body have certain high temperature resistance and high salt resistance performance; a control module is fixedly arranged on the top of the tank cover, and a temperature sensor and an oxygen sensor are fixedly arranged inside the tank cover, and the temperature sensor and the oxygen sensor are both electrically connected to the control module; an electric heating rod is fixedly arranged inside the tank cover, and the electric heating rod extends into the tank body, and the electric heating rod is electrically connected to the control module. The blocks are arranged to be electrically connected; a ventilation structure is fixedly arranged between the outer side of the tank body and the inside of the tank cover, and a stirring assembly is rotatably arranged inside the bottom side of the tank body, and an automatic pressure relief structure is fixedly arranged inside the top side of the tank cover; the automatic pressure relief structure comprises: a fixed cylinder, a sealing groove, a fixed rod, an auxiliary frame and an adjusting screw; the fixed cylinder is fixedly arranged inside the tank cover; the sealing groove is opened inside the top side of the fixed cylinder; the fixed rod is fixedly arranged on the top of the fixed cylinder; the auxiliary frame is fixedly arranged on the top of the fixed rod; the adjusting screw is rotatably arranged between the inside of the auxiliary frame and the inside of the top side of the fixed cylinder, and a knob is fixedly arranged on the top of the adjusting screw.

[0006] Preferably, an attachment protrusion is fixedly provided on the inner wall of the tank body, and an attachment groove is also provided on the inner wall of the tank body, and the attachment groove and the attachment protrusion are both arranged in a ring array.

[0007] Preferably, the ventilation structure includes: an oxygen concentrator, an air outlet pipe, a one-way valve and a connecting pipe; the oxygen concentrator is fixedly arranged on the outside of the tank body, and the oxygen concentrator and the control module are arranged to be electrically connected; one end of the air outlet pipe is fixedly arranged on the outside of the air outlet of the oxygen concentrator; the one-way valve is fixedly arranged on the outside of the other end of the air outlet pipe; the connecting pipe is fixedly arranged on the outside of the top of the one-way valve, and the main body of the connecting pipe is arranged as a U-shaped structure.

[0008] Preferably, the ventilation structure further includes: a fixed tube and an oxygen outlet hole; the fixed tube is fixedly arranged inside the tank cover, and the fixed tube extends into the tank body; the fixed tube is symmetrically arranged in two groups, and the top ends of the two groups of fixed tubes are respectively fixedly connected to the two ends of the connecting tube; the oxygen outlet holes are opened inside both sides of the fixed tube, and the oxygen outlet holes are arranged in a straight line.

[0009] Preferably, the stirring assembly includes: a drive shaft, a fixed disk and a stirring rod; the drive shaft is rotatably arranged inside the bottom side of the tank body, and the drive shaft is fixedly arranged at the shaft end of the motor device, and the motor device is fixedly arranged at the bottom of the tank body; the fixed disk is fixedly arranged at the top end of the drive shaft; the stirring rod is fixedly arranged on the outside of the fixed disk in a circular array, and the stirring rod is arranged in an L shape.

[0010] Preferably, the automatic pressure relief structure also includes: an adjusting frame, a sealing plate and a sealing ring; the adjusting frame is arranged on the outside of the adjusting screw rod through a threaded connection, and the adjusting frame is also slidably arranged on the outside of the fixed rod; the sealing plate is slidably arranged between the adjusting screw rod and the outside of the fixed rod, and the bottom of the sealing plate is in contact with the top of the fixed tube; a spring member is arranged between the top of the sealing plate and the bottom of the adjusting frame, and the spring member is respectively located on the outside of the adjusting screw rod and the fixed rod; the sealing ring is fixedly arranged inside the bottom side of the sealing plate, and the sealing ring is movably arranged inside the sealing groove.

[0011] The utility model is conducive to improving the fermentation tank density of halophilic bacteria and has the following beneficial effects:

[0012] (1) In the fermentation tank of the utility model, the tank cover and the tank body are both made of special alloy materials, so that the tank cover and the tank body can maintain stability for long-term use in a high-salt environment; and the annular array of attachment protrusions and attachment grooves can form a microstructure on the inner wall of the tank body, which is conducive to the attachment and growth of halophilic bacteria; at the same time, the ventilation structure and oxygen sensor can ensure sufficient oxygen supply in the culture solution, providing a fermentation environment that can increase the density of halophilic bacteria;

[0013] (2) In the fermentation tank of the utility model, by providing a fixed plate, a stirring rod and a driving shaft, the culture liquid inside the tank can be stirred, which is conducive to ensuring that the culture liquid is heated evenly and preventing the temperature of the culture liquid far away from the electric heating rod from failing to reach the reproduction temperature of the halophilic bacteria, thereby ensuring the fermentation temperature of the halophilic bacteria;

[0014] (3) In the fermentation tank of the utility model, by providing a sealing plate, a spring member and a fixing rod, the pressure can be automatically relieved when the air pressure inside the tank is too high; this is helpful to prevent the tank from being damaged due to excessive air pressure during the fermentation process; at the same time, by using the adjusting screw rod in conjunction with the fixing rod, the distance between the adjusting frame and the sealing plate can be adjusted, and the initial length of the spring member between the adjusting frame and the sealing plate can be controlled; thus, the pressure relief value can be controlled. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the overall structure of the fermentation tank of the utility model which is beneficial to increasing the density of halophilic bacteria;

[0016] Figure 2 It is a schematic diagram of the cross-sectional structure of the tank body in the fermentation tank of the utility model which is beneficial to improving the density of halophilic bacteria;

[0017] Figure 3 It is a schematic diagram of the internal structure of the tank cover in the fermentation tank of the utility model which is beneficial to increasing the density of halophilic bacteria;

[0018] Figure 4 It is a schematic diagram of the overall structure of the ventilation structure in the fermentation tank of the utility model which is beneficial to increasing the density of halophilic bacteria;

[0019] Figure 5 It is a schematic diagram of the split structure of the automatic pressure relief structure in the fermentation tank of the utility model which is beneficial to increasing the density of halophilic bacteria;

[0020] Figure 6 It is a schematic diagram of the lower surface structure of the sealing plate in the fermentation tank of the utility model which is beneficial to increasing the density of halophilic bacteria;

[0021] In the figure, the corresponding relationship between the component names and the figure numbers is as follows:

[0022] 1. Tank body; 101. Support legs; 102. Tank cover; 103. Control module; 104. Electric heating rod; 105. Temperature sensor; 106. Oxygen sensor; 107. Attachment protrusion; 108. Attachment groove;

[0023] 2. Ventilation structure; 201. Oxygen generator; 202. Air outlet pipe; 203. One-way valve; 204. Connecting pipe; 205. Fixed pipe; 206. Oxygen outlet hole;

[0024] 3. stirring assembly; 301. driving shaft; 302. fixing plate; 303. stirring rod;

[0025] 4. Automatic pressure relief structure; 401. Fixed cylinder; 402. Sealing groove; 403. Fixed rod; 404. Auxiliary frame; 405. Adjusting screw rod; 406. Adjusting frame; 407. Sealing plate; 408. Sealing ring. DETAILED DESCRIPTION

[0026] The implementation of the utility model is further described in detail below in conjunction with the drawings and examples.

[0027] Embodiment 1:

[0028] As attached Figures 1 to 6 As shown: The utility model provides a fermentation tank that is beneficial to increasing the density of halophilic bacteria, comprising: a tank body 1; a supporting leg 101 is fixedly arranged at the bottom of the tank body 1 in a circular array, and a tank cover 102 is fixedly installed on the top of the tank body 1; the tank cover 102 and the tank body 1 are both set to special alloy materials, and the tank cover 102 and the tank body 1 have certain high temperature resistance and high salt resistance performance; a control module 103 is fixedly arranged on the top of the tank cover 102, and a temperature sensor 105 and an oxygen sensor 106 are fixedly arranged inside the tank cover 102, and the temperature sensor 105 and the oxygen sensor 106 are both set to be electrically connected with the control module 103; an electric heating rod 104 is fixedly arranged inside the tank cover 102, and the electric heating rod 104 extends into the tank body 1, and the electric heating rod 104 and the control module 103 are set to be electrically connected. ; A ventilation structure 2 is fixedly arranged between the outer side of the tank body 1 and the inside of the tank cover 102, and a stirring assembly 3 is rotatably arranged inside the bottom side of the tank body 1, and an automatic pressure relief structure 4 is fixedly arranged inside the top side of the tank cover 102; the automatic pressure relief structure 4 includes: a fixed cylinder 401, a sealing groove 402, a fixed rod 403, an auxiliary frame 404, an adjusting screw rod 405, an adjusting frame 406, a sealing plate 407 and a sealing ring 408; the fixed cylinder 401 is fixedly arranged inside the tank cover 102; the sealing groove 402 is opened inside the top side of the fixed cylinder 401; the fixed rod 403 is fixedly arranged at the top of the fixed cylinder 401; the auxiliary frame 404 is fixedly arranged at the top of the fixed rod 403; the adjusting screw rod 405 is rotatably arranged between the inside of the auxiliary frame 404 and the inside of the top side of the fixed cylinder 401, and a knob is fixedly arranged on the top of the adjusting screw rod 405;

[0029] Among them, the adjusting frame 406 is arranged on the outside of the adjusting screw rod 405 through a threaded connection, and the adjusting frame 406 is also slidably arranged on the outside of the fixing rod 403; the sealing plate 407 is slidably arranged between the adjusting screw rod 405 and the outside of the fixing rod 403, and the bottom of the sealing plate 407 is in contact with the top of the fixing cylinder 401; a spring member is arranged between the top of the sealing plate 407 and the bottom of the adjusting frame 406, and the spring members are respectively located on the outside of the adjusting screw rod 405 and the fixing rod 403; the sealing ring 408 is fixedly arranged inside the bottom side of the sealing plate 407, and the sealing ring 408 is movably arranged inside the sealing groove 402; its specific function is: by arranging the sealing plate 407, the spring member and the fixing rod 403, it can automatically release the pressure when the air pressure inside the tank body 1 is too high; it is beneficial to prevent the tank body 1 from being damaged due to excessive air pressure during the fermentation process.

[0030] Embodiment 2:

[0031] As attached Figure 1 , 2 4: On the basis of the first embodiment, an attachment protrusion 107 is fixedly provided on the inner wall of the tank body 1, and an attachment groove 108 is also provided on the inner wall of the tank body 1, and the attachment groove 108 and the attachment protrusion 107 are arranged in a ring array; the ventilation structure 2 includes: an oxygen generator 201, an air outlet pipe 202, a one-way valve 203, a connecting pipe 204, a fixed pipe 205 and an oxygen outlet hole 206; the oxygen generator 201 is fixedly provided on the outside of the tank body 1, and the oxygen generator 201 and the control module 103 are arranged to be electrically connected; one end of the air outlet pipe 202 is fixedly provided on the outside of the air outlet of the oxygen generator 201; the one-way valve 203 is fixedly provided on the outside of the other end of the air outlet pipe 202; the connecting pipe 204 is fixedly provided on The outside of the top of the one-way valve 203 and the main body of the connecting pipe 204 are arranged in a U-shaped structure; the fixed pipe 205 is fixedly arranged inside the tank cover 102, and the fixed pipe 205 extends into the tank body 1; the fixed pipe 205 is symmetrically arranged in two groups, and the tops of the two groups of fixed pipes 205 are respectively fixedly connected to the two ends of the connecting pipe 204; the oxygen outlet holes 206 are opened inside the two sides of the fixed pipe 205, and the oxygen outlet holes 206 are arranged in a straight line; its specific function is: by using the annular array attachment protrusions 107 and the attachment grooves 108, the inner wall of the tank body 1 can form a microstructure, which is conducive to the attachment and growth of halophilic bacteria; at the same time, by using the ventilation structure 2 and the oxygen sensor 106, it can ensure that the oxygen in the culture medium is fully supplied.

[0032] Embodiment three:

[0033] As attached Figure 2As shown: On the basis of Example 1 and Example 2, the stirring assembly 3 includes: a driving shaft 301, a fixed disk 302 and a stirring rod 303; the driving shaft 301 is rotatably arranged inside the bottom side of the tank body 1, and the driving shaft 301 is fixedly arranged on the shaft end of the motor device, and the motor device is fixedly arranged at the bottom of the tank body 1; the fixed disk 302 is fixedly arranged on the top of the driving shaft 301; the stirring rod 303 is fixedly arranged on the outside of the fixed disk 302 in a circular array, and the stirring rod 303 is arranged in an L shape; its specific function is: by arranging the fixed disk 302, the stirring rod 303 and the driving shaft 301, the culture liquid inside the tank body 1 can be stirred, which is beneficial to ensure that the culture liquid is heated evenly.

[0034] The working process and specific use method of the fermentation tank embodiment of the utility model which is beneficial to improving the density of halophilic bacteria are as follows: when the fermentation tank of the utility model which is beneficial to improving the density of halophilic bacteria is used, the culture solution and the halophilic bacteria are introduced into the interior of the tank body 1, and then the culture solution is heated by the electric heating rod 104; at the same time, the motor device is started to drive the driving shaft 301 and the fixed disk 302 to rotate, so that the fixed disk 302 stirs the culture solution through the stirring rod 303, so that the culture solution is heated evenly, and when the temperature reaches a predetermined value, the temperature sensor 105 transmits a temperature signal to the control module 103, and the control module 103 controls the electric heating rod 104 to stop working; when the oxygen concentration inside the tank body 1 is insufficient, the oxygen sensor 106 transmits a signal to the control module 103, and the control module 103 controls the oxygen generator 201 to work, so that oxygen passes through the outlet pipe 202 and the one-way valve 203 and the connecting pipe 2 04 enters the fixed tube 205, and enters the culture solution through the oxygen outlet 206, so that the oxygen concentration inside the tank body 1 reaches a predetermined value; during the fermentation process, when the air pressure inside the tank body 1 is too high, the air pressure will push the sealing plate 407 open for automatic pressure relief; when the air pressure inside the tank body 1 is normal, the spring member drives the sealing plate 407 to reset, so that the sealing ring 408 slides into the sealing groove 402, so that the tank body 1 is restored to a closed state; the adjusting screw rod 405 is rotated, and the adjusting screw rod 405 drives the adjusting frame 406 to move up and down through the fixed rod 403, so that the distance between the adjusting frame 406 and the sealing plate 407 is adjusted, and then the initial length of the spring member between the adjusting frame 406 and the sealing plate 407 is adjusted; and then the pressure relief value can be controlled; the annular array attachment protrusions 107 and the attachment grooves 108 can form a microstructure on the inner wall of the tank body 1, which is conducive to the attachment and growth of halophilic bacteria.

Claims

1. A fermentation tank that is beneficial to increasing the density of halophilic bacteria, characterized in that: include: A tank body (1); the bottom of the tank body (1) is fixedly provided with support legs (101) in a circular array, and the top of the tank body (1) is fixedly installed with a tank cover (102); a control module (103) is fixedly provided on the top of the tank cover (102), and a temperature sensor (105) and an oxygen sensor (106) are fixedly provided inside the tank cover (102), and the temperature sensor (105) and the oxygen sensor (106) are both electrically connected to the control module (103); an electric heating rod (104) is fixedly provided inside the tank cover (102), and the electric heating rod (104) extends into the interior of the tank body (1), and the electric heating rod (104) and the control module (103) are electrically connected; a ventilation structure (2) is fixedly provided between the outside of the tank body (1) and the inside of the tank cover (102), A stirring assembly (3) is rotatably arranged inside the bottom side of the tank body (1), and an automatic pressure relief structure (4) is fixedly arranged inside the top side of the tank cover (102); the automatic pressure relief structure (4) comprises: a fixed cylinder (401), a sealing groove (402), a fixed rod (403), an auxiliary frame (404) and an adjusting screw (405); the fixed cylinder (401) is fixedly arranged inside the tank cover (102); the sealing groove (402) is opened inside the top side of the fixed cylinder (401); the fixed rod (403) is fixedly arranged at the top of the fixed cylinder (401); the auxiliary frame (404) is fixedly arranged at the top of the fixed rod (403); the adjusting screw (405) is rotatably arranged between the inside of the auxiliary frame (404) and the inside of the top side of the fixed cylinder (401), and a knob is fixedly arranged at the top of the adjusting screw (405).

2. A fermentation tank that is beneficial to increasing the density of halophilic bacteria according to claim 1, characterized in that: An attachment protrusion (107) is fixedly arranged on the inner wall of the tank body (1), and an attachment groove (108) is also provided on the inner wall of the tank body (1), and the attachment groove (108) and the attachment protrusion (107) are both arranged in a ring array.

3. A fermentation tank that is beneficial to increasing the density of halophilic bacteria according to claim 1 or 2, characterized in that: The ventilation structure (2) comprises: an oxygen concentrator (201), an air outlet pipe (202), a one-way valve (203) and a connecting pipe (204); the oxygen concentrator (201) is fixedly arranged outside the tank body (1), and the oxygen concentrator (201) and the control module (103) are arranged to be electrically connected; one end of the air outlet pipe (202) is fixedly arranged outside the air outlet of the oxygen concentrator (201); the one-way valve (203) is fixedly arranged outside the other end of the air outlet pipe (202); the connecting pipe (204) is fixedly arranged outside the top of the one-way valve (203), and the main body of the connecting pipe (204) is arranged in a U-shaped structure.

4. A fermentation tank that is beneficial to increasing the density of halophilic bacteria according to claim 3, characterized in that: The ventilation structure (2) further comprises: a fixed tube (205) and an oxygen outlet hole (206); the fixed tube (205) is fixedly arranged inside the tank cover (102), and the fixed tube (205) extends into the tank body (1); two groups of fixed tubes (205) are symmetrically arranged, and the top ends of the two groups of fixed tubes (205) are respectively fixedly connected to the two ends of the connecting tube (204); the oxygen outlet holes (206) are opened inside both sides of the fixed tube (205), and the oxygen outlet holes (206) are arranged in a straight line.

5. A fermentation tank that is beneficial to increasing the density of halophilic bacteria according to claim 1 or 2, characterized in that: The stirring assembly (3) comprises: a driving shaft (301), a fixed disk (302) and a stirring rod (303); the driving shaft (301) is rotatably arranged inside the bottom side of the tank body (1), and the driving shaft (301) is fixedly arranged on the shaft end of the motor device, and the motor device is fixedly arranged on the bottom of the tank body (1); the fixed disk (302) is fixedly arranged on the top of the driving shaft (301); the stirring rod (303) is fixedly arranged on the outside of the fixed disk (302) in a ring array, and the stirring rod (303) is arranged in an L shape.

6. A fermentation tank that is beneficial to increasing the density of halophilic bacteria according to claim 1 or 2, characterized in that: The automatic pressure relief structure (4) further comprises: an adjusting frame (406), a sealing plate (407) and a sealing ring (408); the adjusting frame (406) is arranged on the outside of the adjusting screw rod (405) by means of a threaded connection, and the adjusting frame (406) is also slidably arranged on the outside of the fixing rod (403); the sealing plate (407) is slidably arranged between the adjusting screw rod (405) and the outside of the fixing rod (403), and the bottom of the sealing plate (407) is in contact with the top of the fixing cylinder (401); a spring member is arranged between the top of the sealing plate (407) and the bottom of the adjusting frame (406), and the spring member is respectively located on the outside of the adjusting screw rod (405) and the fixing rod (403); the sealing ring (408) is fixedly arranged inside the bottom side of the sealing plate (407), and the sealing ring (408) is movably arranged inside the sealing groove (402).

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