ELISA kit for animal husbandry detection

Abstract

The utility model discloses an ELISA (Enzyme Linked Immunosorbent Assay) kit for animal husbandry detection. The ELISA kit comprises a box body, the annular block is fixedly mounted at the axis of the box body, and a second filter membrane is fixedly mounted in the annular block; the box cover is rotationally arranged at the top end of the box body; and the annular cover plate is fixedly mounted at the axis of the box cover and abuts against the annular block. According to the novel cast-off cell embedding box provided by the utility model, the second filter membrane is arranged in the annular block, so that cells do not need to be independently wrapped with the filter membranes, the situation that filter paper bags folded by experimenters are different in size, and the subsequent manufacturing process is influenced is avoided, and when the cells are put into the annular block, the box cover is rotated to be closed with the box body; the annular cover plate installed in the box cover abuts against the annular block, cells are prevented from being separated from the annular block in the subsequent processing, meanwhile, the distance between the first filter membrane and the second filter membrane installed in the annular cover plate is 2 cm, the cell clustering effect in the subsequent processing is better, the influence of different sizes of manually folded filter paper bags is avoided, and the cell clustering efficiency is improved. And the processing efficiency of the cell wax block can be improved.

Description

Technical Field

[0001] The utility model relates to the technical field of medical equipment, in particular to an ELISA kit for animal husbandry detection. Background Art

[0002] Exfoliated cell pathology examination is a method of collecting and testing livestock exfoliated cells to determine benign and malignant lesions of livestock cells. The commonly used examination method is to make cells into cell wax blocks, and then slice and examine the cell wax blocks to achieve the purpose of examination. However, the cells need to be extracted from body fluids to make cell wax blocks. The traditional extraction method is to centrifuge the body fluid containing exfoliated cells to separate the cells and the clear liquid, and then collect the separated cells by folding the filter paper into a small bag, and put them into a tissue embedding box for subsequent fixation, dehydration, transparency, wax dipping and embedding. However, the filter paper bags folded by the experimenters are of different sizes. If the filter paper bag is too large and thick, the lid of the tissue embedding box will collapse during subsequent operations, and the filter paper with the cell specimen will fall out of the embedding box, causing contamination of the specimen, thereby affecting the subsequent processing technology. Utility Model Content

[0003] The utility model aims to provide an ELISA kit for animal husbandry detection, which is used to solve the above problems.

[0004] In order to achieve the above object, the utility model provides the following technical solution: an ELISA kit for livestock detection, comprising:

[0005] Box;

[0006] An annular block is fixedly installed at the axis of the box body, and a second filter membrane is fixedly installed in the annular block;

[0007] Rotate the box cover disposed on the top of the box body;

[0008] An annular cover plate is fixedly mounted on the axis of the box cover, the annular cover plate abuts against the annular block, and a first filter membrane is fixedly mounted inside the annular cover plate.

[0009] Preferably, the box body is provided with reinforcing ribs in a circumferential array, and the side walls of the reinforcing ribs are fixedly connected to the annular block.

[0010] Preferably, a rotating shaft is symmetrically arranged in the box body, and a box cover is movably mounted on the side wall of the rotating shaft.

[0011] Preferably, a groove is formed on the side wall of the box body, and a clamping block is fixedly installed in the groove.

[0012] Preferably, a locking block is fixedly mounted on the bottom end of the box cover, and one end of the locking block is clamped to the clamping block.

[0013] Preferably, the top of the box cover is provided with through holes in a linear array.

[0014] In the above technical scheme, an ELISA kit for livestock detection provided by the utility model has the following beneficial effects: by installing an annular block in the box body, the cells placed in the box body can be concentrated in the annular block for subsequent work such as reagent immersion, dehydration and embedding; by arranging a second filter membrane in the annular block, the cells do not need to be wrapped with a filter membrane separately to avoid the experimenter folding filter paper bags of different sizes, which affects the subsequent production process; when the cells are placed in the annular block, the box cover is closed by rotating the box body so that the annular cover plate installed in the box cover abuts against the annular block to avoid the cells from detaching from the annular block in subsequent processing; at the same time, the spacing between the first filter membrane and the second filter membrane installed in the annular cover plate is 2 cm, so that the cell agglomeration effect is better in subsequent processing, which not only avoids the influence of the different sizes of manually folded filter paper bags, but also improves the processing efficiency of cell wax blocks. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present utility model. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0016] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0017] Figure 2 It is a schematic diagram of the cross-sectional structure of the utility model.

[0018] Description of reference numerals:

[0019] 1. Box body; 2. Box cover; 3. Groove; 4. Block; 5. Locking block; 6. Ring cover; 7. First filter membrane; 8. Ring block; 9. Second filter membrane; 10. Reinforcing rib; 11. Rotating shaft; 12. Through hole. DETAILED DESCRIPTION

[0020] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0021] like Figure 1-2 As shown, an ELISA kit for animal husbandry detection comprises:

[0022] Box 1;

[0023] An annular block 8 is fixedly mounted at the axis of the housing 1, and a second filter membrane 9 is fixedly mounted in the annular block 8;

[0024] Rotate the box cover 2 disposed on the top of the box body 1;

[0025] An annular cover plate 6 is fixedly mounted on the axis of the box cover 2 . The annular cover plate 6 abuts against an annular block 8 . A first filter membrane 7 is fixedly mounted inside the annular cover plate 6 .

[0026] Specifically, by arranging a second filter membrane 9 in the annular block 8 installed at the axis of the box body 1, the cells separated by the centrifuge can be directly placed in the annular block 8 for collection, thereby avoiding the experimenter from folding the filter paper bag to collect the cells and preventing the folded filter paper bag from being too large to break the embedding box. When the cells are placed in the annular block 8, the box cover 2 is driven to rotate so that the box cover 2 and the box body 1 are closed. At the same time, the annular cover plate 6 installed in the box cover 2 can abut against the annular block 8 to prevent the cells from detaching from the annular block 8 in subsequent processing. When the annular cover plate 6 abuts against the annular block 8, the distance between the first filter membrane 7 installed in the annular cover plate 6 and the second filter membrane 9 provided in the annular block 8 is maintained at two centimeters, so that the efficiency of cell clustering is higher.

[0027] In the above embodiment, by installing an annular block 8 in the box body 1, the cells placed in the box body 1 can be concentrated in the annular block 8 for subsequent work such as reagent immersion, dehydration and embedding. By arranging a second filter membrane 9 in the annular block 8, the cells do not need to be wrapped in a filter membrane separately to avoid the experimenter folding filter paper bags of different sizes, which affects the subsequent production process. When the cells are placed in the annular block 8, the box cover 2 is rotated to close the box body 1, so that the annular cover plate 6 installed in the box cover 2 abuts against the annular block 8 to prevent the cells from detaching from the annular block 8 during subsequent processing. At the same time, the spacing between the first filter membrane 7 and the second filter membrane 9 installed in the annular cover plate 6 is 2 cm, so that the cell agglomeration effect is better in subsequent processing, which not only avoids the influence of the different sizes of manually folded filter paper bags, but also improves the processing efficiency of cell wax blocks.

[0028] As an embodiment further provided by the present invention, reinforcing ribs 10 in a circumferential array are arranged in the box body 1 , and the side walls of the reinforcing ribs 10 are fixedly connected to the annular block 8 .

[0029] Specifically, by providing reinforcing ribs 10 in the box body 1 to connect with the side walls of the annular block 8, the annular block 8 is more stably fixed in the box body 1, thereby improving the stability of the annular block 8 in subsequent processing.

[0030] As an embodiment further provided by the present invention, a rotating shaft 11 is symmetrically arranged in the box body 1, and a box cover 2 is movably mounted on the side wall of the rotating shaft 11.

[0031] Specifically, the box cover 2 is connected to the rotating shaft 11 provided in the box body 1 , so that the box cover 2 can rotate along the rotating shaft 11 and close with the box body 1 when driven.

[0032] As another embodiment further provided by the present invention, a groove 3 is formed on the side wall of the box body 1 , and a clamping block 4 is fixedly installed in the groove 3 .

[0033] As another embodiment further provided by the present invention, a locking block 5 is fixedly installed at the bottom end of the box cover 2 , and one end of the locking block 5 is clamped to the clamping block 4 .

[0034] Specifically, when the box cover 2 is closed with the box body 1, the locking block 5 installed at the bottom of the box cover 2 extends into the groove 3 and engages with the locking block 4, so that the box cover 2 can be fixed on the box body 1, preventing the box cover 2 from detaching from the box body 1 during processing and causing cell contamination.

[0035] As another embodiment further provided by the present invention, the top of the box cover 2 is provided with through holes 12 in a linear array.

[0036] Specifically, when the cells need to be treated with reagents, the through holes 12 are opened on the box cover 2 so that the reagents can enter the box body 1 to treat the cells.

[0037] Working principle: By setting the second filter membrane 9 in the annular block 8, the cells can be directly placed in the annular block 8 for collection, avoiding the experimenter from folding the filter paper separately to collect the cells. When the cells are placed in the annular block 8, the box cover 2 is driven to rotate along the rotating shaft 11, so that the box cover 2 and the box body 1 are closed, and the annular cover plate 6 and the annular block 8 are abutted against each other, thereby preventing the cells from escaping from the annular block 8 in subsequent processing. When the annular cover plate 6 and the annular block 8 are abutted, the distance between the first filter membrane 7 and the second filter membrane 9 installed in the annular cover plate 6 is 2 cm, so that the cells between the first filter membrane 7 and the second filter membrane 9 can quickly form cell clusters in subsequent processing, and the reinforcing ribs 10 set in the box body 1 are used to improve the stability of the annular block 8 in the subsequent processing. When it is necessary to use a reagent to treat the cells, the reagent can be allowed to pass through the through hole 12 into the box body 1 to treat the cells by directly soaking the box body 1 in the reagent.

[0038] The above only describes some exemplary embodiments of the present invention by way of illustration. It is undoubted that those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. An ELISA kit for livestock detection, characterized in that: include: Box body (1); An annular block (8) is fixedly mounted at the axis of the housing (1), wherein a second filter membrane (9) is fixedly mounted in the annular block (8); Rotating a box cover (2) disposed on the top of the box body (1); An annular cover plate (6) is fixedly mounted on the axis of the box cover (2), the annular cover plate (6) abuts against an annular block (8), and a first filter membrane (7) is fixedly mounted inside the annular cover plate (6).

2. An ELISA kit for animal husbandry detection according to claim 1, characterized in that: The box body (1) is provided with reinforcing ribs (10) in a circumferential array, and the side walls of the reinforcing ribs (10) are fixedly connected to the annular block (8).

3. An ELISA kit for animal husbandry detection according to claim 1, characterized in that: A rotating shaft (11) is symmetrically arranged in the box body (1), and a box cover (2) is movably mounted on the side wall of the rotating shaft (11).

4. An ELISA kit for animal husbandry detection according to claim 1, characterized in that: A groove (3) is provided on the side wall of the box body (1), and a clamping block (4) is fixedly installed in the groove (3).

5. An ELISA kit for animal husbandry detection according to claim 1, characterized in that: A locking block (5) is fixedly mounted on the bottom end of the box cover (2), and one end of the locking block (5) is clamped on the clamping block (4).

6. An ELISA kit for livestock detection according to claim 1, characterized in that: The top end of the box cover (2) is provided with through holes (12) in a linear array.

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