A nano antibody purification device

Abstract

The utility model discloses a purification device for nano antibodies, including a tank body, a feed pipe and a protective cover are installed on the top of the tank body, and a reduction motor is installed on the top of the protective cover, two groups of filter plates are installed inside the tank body, and sealing rings are installed at the edges of the filter plates, and a pull rod is installed at the center of the filter plates. The utility model achieves an anti-blocking effect by providing a reduction motor, a protective cover, a pull rod, a drive shaft, a slide groove and a convex column. The operation of the reduction motor will drive the drive shaft to rotate, and cooperate with the sliding connection between the slide groove and the convex column to indirectly drive the pull rod to move vertically, pull the two groups of filter plates to move vertically, drive the flow of materials, and accelerate the filtration of materials. At the same time, the reciprocating movement of the filter plate will vibrate out the materials blocked in the hole to avoid the blockage of the filter plate affecting the filtering effect, so that the passivation device has an anti-blocking function.

Description

Technical Field

[0001] The utility model relates to the technical field of purification devices for antibodies, in particular to a purification device for nano antibodies. Background Art

[0002] Nanobodies are a special kind of antibody fragments, which are derived from the blood of camelids (such as camels, alpacas and their close relatives). The molecular weight of nanobodies is extremely small, only 15KD (kilodaltons), and the VHH crystal size is 2.5 nanometers (millimometers) and 4 nanometers long, so they are vividly called "nanobodies". Antibody purification is a biotechnology used to separate and extract antibody molecules. In the process of antibody passivation, a purification device is usually used, and after filtration passivation, large-diameter substances in the material will be blocked in the mesh, which reduces the filtration passivation speed and affects the passivation efficiency. Therefore, a purification device for nanobodies is now provided. Utility Model Content

[0003] The purpose of the utility model is to provide a nano antibody purification device to solve the problems raised in the above background technology.

[0004] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a nano antibody purification device, comprising a tank body, a feed pipe and a protective cover are installed on the top of the tank body, and a reduction motor is installed on the top of the protective cover, two groups of filter plates are installed inside the tank body, and sealing rings are installed at the edges of the filter plates, a pull rod is installed at the center of the filter plate, and the pull rod passes through the top of the tank body, a convex column is arranged on the outer side of the top of the pull rod, a drive shaft is arranged on one side of the convex column, and a fixed connection is formed between the top of the drive shaft and the reduction motor, a slide groove is arranged on the outer side of the drive shaft, a support frame is installed on the outer side of the bottom end of the tank body, a passivation tube is arranged at the bottom end of the tank body, and a discharge port is arranged at the bottom end of the passivation tube.

[0005] Preferably, the top end of the pull rod is flat, and an integrated structure is formed between the pull rod and the boss, and a movable connection is formed between the boss and the slide groove.

[0006] Preferably, a sieve plate is provided inside the passivation tube, and a sealing plate is installed on one side of the sieve plate, a handle is provided on the outside of the sealing plate, a fixing plate is provided on the outside of the passivation tube, mounting plates are provided on both sides of the sealing plate, and fixing bolts are installed inside the mounting plate and the fixing plate.

[0007] Preferably, the top and bottom ends of the sieve plate are both provided with sealing convex rings, and the sealing convex rings are fixed on the inner side wall of the passivation tube, and the interior of the passivation tube is provided with a mounting groove that matches the sieve plate.

[0008] Preferably, three groups of sieve plates are provided, and the sieve plates are arranged and distributed at equal intervals inside the passivation tube.

[0009] Preferably, a heating pipe is installed on the outer side of the tank body, and water inlet pipes are provided at the top and bottom ends of the heating pipe.

[0010] Preferably, the heating pipe is distributed in a spirally wound shape on the outside of the tank body, and an integrated structure is formed between the heating pipe and the tank body.

[0011] Preferably, thermal insulation cotton is arranged on the outside of the heating pipe, and a thermal insulation shell is arranged on the outside of the thermal insulation cotton, and a fixed connection is formed between the thermal insulation shell and the tank body.

[0012] Compared with the prior art, the beneficial effects of the utility model are:

[0013] (1) The anti-blocking effect is achieved by providing a reduction motor, a protective cover, a pull rod, a drive shaft, a slide groove and a convex column. The operation of the reduction motor will drive the drive shaft to rotate, and cooperate with the sliding connection between the slide groove and the convex column to indirectly drive the pull rod to move vertically, pull the two sets of filter plates to move vertically, drive the flow of materials, and accelerate the filtration of materials. At the same time, the reciprocating movement of the filter plate will vibrate out the materials blocked in the holes, avoiding the blockage of the filter plate affecting the filtering effect, so that the passivation device has an anti-blocking function;

[0014] (2) By providing a sealing plate, a discharge port, a passivation tube, a fixing plate, a mounting plate, a fixing bolt, a sealing convex ring, a sieve plate and a handle, the sieve plate can be quickly maintained. The sealing plate can seal the mounting groove on the passivation tube, and the distribution of the fixing plate, the mounting plate and the fixing bolt can lock the position of the sealing plate, which is convenient for disassembly and installation of the sealing plate, and convenient for taking out the sieve plate from the inside of the passivation tube, cleaning or replacing the sieve plate, thereby improving the flexibility of use;

[0015] (3) The water inlet pipe, the heat-insulating shell, the heat-insulating cotton, the sealing ring and the heating pipe are provided to realize the heating function. The distribution of the water inlet pipe and the heating pipe can input the heat source into the interior of the heating pipe, thereby heating the tank body and making the interior of the tank body reach the specified temperature requirement. The distribution of the heat-insulating shell and the heat-insulating cotton can reduce the temperature loss and improve the heat-insulating effect, so that the passivation device has the heating function. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0017] Figure 1 This is a front view structural schematic diagram of the utility model;

[0018] Figure 2 It is a side view structural schematic diagram of the utility model;

[0019] Figure 3 It is a front view cross-sectional structural schematic diagram of the utility model;

[0020] Figure 4 This is a schematic diagram of a partial cross-sectional structure of the protective cover of the utility model;

[0021] Figure 5 It is a schematic diagram of a partial cross-sectional structure of a passivation tube of the utility model in a front view.

[0022] Description of reference numerals in the figures:

[0023] 1. Tank body; 2. Feed pipe; 3. Speed ​​reducer motor; 4. Protective cover; 5. Water inlet pipe; 6. Insulation shell; 7. Support frame; 8. Sealing plate; 9. Discharge port; 10. Passivation tube; 11. Fixing plate; 12. Mounting plate; 13. Fixing bolt; 14. Pull rod; 15. Filter plate; 16. Insulation cotton; 17. Sealing convex ring; 18. Sealing ring; 19. Heating pipe; 20. Drive shaft; 21. Slide; 22. Boss; 23. Sieve plate; 24. Handle. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical solution and advantages of the embodiment of the utility model clearer, the technical solution in the embodiment of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiment of the utility model. Obviously, the described embodiment is a part of the embodiment of the utility model, not all the embodiments. Based on the embodiment of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0025] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0026] See also Figure 1-5The utility model provides an embodiment: a nano antibody purification device, comprising a tank body 1, a feed pipe 2 and a protective cover 4 are installed at the top of the tank body 1, and a reduction motor 3 is installed at the top of the protective cover 4, two sets of filter plates 15 are installed inside the tank body 1, and a sealing ring 18 is installed at the edge of the filter plate 15, a pull rod 14 is installed at the center of the filter plate 15, and the pull rod 14 runs through the top of the tank body 1, a convex column 22 is arranged on the outside of the top of the pull rod 14, and a drive shaft 22 is arranged on one side of the convex column 22. A driving shaft 20 is provided, and a fixed connection is formed between the top end of the driving shaft 20 and the reduction motor 3, a slide groove 21 is provided on the outside of the driving shaft 20, a support frame 7 is installed on the outside of the bottom end of the tank body 1, a passivation tube 10 is provided at the bottom end of the tank body 1, and a discharge port 9 is provided at the bottom end of the passivation tube 10, the top end of the pull rod 14 is flat, and an integrated structure is formed between the pull rod 14 and the convex column 22, and a movable connection is formed between the convex column 22 and the slide groove 21, and the rotation of the driving shaft 20 can drive the pull rod 14 to move vertically;

[0027] Specifically, as shown in the figure, when in use, the operation of the reduction motor 3 drives the drive shaft 20 to rotate, and cooperates with the sliding connection between the slide groove 21 and the protruding column 22 to indirectly drive the pull rod 14 to move vertically, pull the two sets of filter plates 15 to perform vertical reciprocating motion, and vibrate out the materials blocked in the holes, so as to avoid the blockage of the filter plate 15 affecting the filtering effect;

[0028] A sieve plate 23 is provided inside the passivation tube 10, and a sealing plate 8 is installed on one side of the sieve plate 23, and a handle 24 is provided on the outside of the sealing plate 8. A fixing plate 11 is provided on the outside of the passivation tube 10, and mounting plates 12 are provided on both sides of the sealing plate 8, and fixing bolts 13 are installed inside the mounting plate 12 and the fixing plate 11. The top and bottom ends of the sieve plate 23 are provided with sealing convex rings 17, and the sealing convex rings 17 are fixed on the inner side wall of the passivation tube 10. The inside of the passivation tube 10 is provided with a mounting groove matched with the sieve plate 23. Three groups of sieve plates 23 are provided, and the sieve plates 23 are arranged and distributed at equal intervals inside the passivation tube 10. The sealing plate 8 can seal the mounting groove on the passivation tube 10;

[0029] Specifically, as shown in the figure, when in use, the position of the sealing plate 8 can be locked through the distribution of the fixing plate 11, the mounting plate 12 and the fixing bolt 13, so as to facilitate the disassembly and installation of the sealing plate 8, and to facilitate the removal of the sieve plate 23 from the interior of the passivation tube 10, and to clean or replace the sieve plate 23;

[0030] A heating pipe 19 is installed on the outside of the tank body 1, and a water inlet pipe 5 is provided at the top and bottom of the heating pipe 19. The heating pipe 19 is distributed in a spiral winding shape on the outside of the tank body 1, and an integrated structure is formed between the heating pipe 19 and the tank body 1. A heat preservation cotton 16 is arranged on the outside of the heating pipe 19, and a heat preservation shell 6 is arranged on the outside of the heat preservation cotton 16. A fixed connection is formed between the heat preservation shell 6 and the tank body 1, which increases the thickness of the tank body and improves the heat insulation effect.

[0031] Specifically, as shown in the figure, when in use, the heat source is input into the interior of the heating pipe 19 through the distribution of the water inlet pipe 5 and the heating pipe 19, the temperature of the tank body 1 can be increased, so that the interior of the tank body 1 reaches the specified temperature requirement, and the distribution of the insulation shell 6 and the insulation cotton 16 reduces the temperature loss.

[0032] Working principle: When in use, first, the purification device is moved to the target area for placement, and the water inlet pipe 5 is connected to the heat source, the heating medium is controlled to be transported to the inside of the heating pipe 19, the tank body 1 is heated, so that the inside of the tank body 1 reaches the specified temperature requirement, and the material to be processed is injected into the inside of the tank body 1 from the feed pipe 2, and the reduction motor 3 is controlled to operate, the drive shaft 20 is driven to rotate, and the sliding connection between the slide groove 21 and the protrusion 22 is cooperated to indirectly drive the pull rod 14 to move vertically, pull the two sets of filter plates 15 to move vertically, drive the material flow, and accelerate the filtration of the material. At the same time, the reciprocating movement of the filter plate 15 will vibrate out the material blocked in the hole to avoid the blockage of the filter plate 15 affecting the filtering effect. Then, when the sieve plate 23 needs to be maintained, the corresponding fixing bolt 13 is removed, and the handle 24 is grabbed, and the sieve plate 23 is forcefully pulled out from the inside of the passivation tube 10, so that the sieve plate 23 can be cleaned or replaced, and the use of the purification device is finally completed.

[0033] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

Claims

1. A nano-antibody purification device, comprising a tank (1), characterized in that: A feed pipe (2) and a protective cover (4) are installed at the top of the tank body (1), and a reduction motor (3) is installed at the top of the protective cover (4). Two groups of filter plates (15) are installed inside the tank body (1), and a sealing ring (18) is installed at the edge of the filter plate (15). A pull rod (14) is installed at the center of the filter plate (15), and the pull rod (14) passes through the top of the tank body (1). A boss (22) is arranged on the outer side of the top of the pull rod (14), a drive shaft (20) is arranged on one side of the boss (22), and a fixed connection is formed between the top of the drive shaft (20) and the reduction motor (3). A slide groove (21) is arranged on the outer side of the drive shaft (20). A support frame (7) is installed on the outer side of the bottom end of the tank body (1). A passivation tube (10) is arranged at the bottom end of the tank body (1), and a discharge port (9) is arranged at the bottom end of the passivation tube (10).

2. A nanobody purification device according to claim 1, characterized in that: The top end of the pull rod (14) is flat, and an integrated structure is formed between the pull rod (14) and the convex column (22), and a movable connection is formed between the convex column (22) and the slide groove (21).

3. A nanobody purification device according to claim 1, characterized in that: A sieve plate (23) is arranged inside the passivation tube (10), and a sealing plate (8) is installed on one side of the sieve plate (23), and a handle (24) is arranged on the outer side of the sealing plate (8). A fixing plate (11) is arranged on the outer side of the passivation tube (10), and mounting plates (12) are arranged on both sides of the sealing plate (8), and fixing bolts (13) are installed inside the mounting plate (12) and the fixing plate (11).

4. A nanobody purification device according to claim 3, characterized in that: The top and bottom ends of the sieve plate (23) are both provided with sealing convex rings (17), and the sealing convex rings (17) are fixed on the inner wall of the passivation tube (10). The interior of the passivation tube (10) is provided with a mounting groove that matches the sieve plate (23).

5. A nanobody purification device according to claim 3, characterized in that: The sieve plates (23) are provided in three groups, and the sieve plates (23) are arranged and distributed at equal intervals inside the passivation tube (10).

6. A nanobody purification device according to claim 1, characterized in that: A heating pipe (19) is installed on the outer side of the tank body (1), and water inlet pipes (5) are arranged at the top and bottom ends of the heating pipe (19).

7. A nanobody purification device according to claim 6, characterized in that: The heating pipe (19) is distributed in a spirally wound shape on the outside of the tank body (1), and an integrated structure is formed between the heating pipe (19) and the tank body (1).

8. A nanobody purification device according to claim 6, characterized in that: The outer side of the heating pipe (19) is provided with heat-insulating cotton (16), the outer side of the heat-insulating cotton (16) is provided with a heat-insulating shell (6), and a fixed connection is formed between the heat-insulating shell (6) and the tank body (1).

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