Liquid specimen splitting detection device

Abstract

The utility model discloses a liquid specimen cracking detection device. The device comprises a detection unit, a cracking unit and a detachable limiting structure. The detection unit comprises a detection shell, a sample collection unit and a detection reagent strip. The splitting unit comprises a base, a piercing sheath, a sealing film and a bottom bottle. The detachable limiting structure is installed between the detection unit and the splitting unit and used for limiting the depth of the portion, inserted into the top opening of the base, of the detection unit in the initial state, and the piercing sheath is prevented from piercing the sealing film. In a detection state, the detachable limiting structure is detached, and the piercing sheath pierces the sealing film under the pushing of the insertion section of the detection shell; and the sample collection unit extends into the bottom bottle. Under the condition that the sample collection unit and the detection reagent strip are integrated in the long-strip-shaped detection shell, the liquid sample and the lysate can be mixed and the mixed liquid can be conveyed to the reagent strip only through one downward pressing action in cooperation with the lysate unit.

Description

Technical Field

[0001] The utility model belongs to the technical field of liquid specimen detection, and in particular relates to a liquid specimen lysis detection device. Background Art

[0002] In the prior art, when liquid specimens such as saliva are not lysed with lysis solution, the amount of saliva required for detection cannot be reached due to the small amount of saliva in some people, which affects the detection results. When lysis with lysis solution, the saliva and lysis solution need to be mixed and then dripped into the detection device, which is relatively complicated to operate and the transfer process increases the risk of sample contamination.

[0003] The patent with publication number CN220194900U, entitled "An integrated device for collecting, storing and detecting body fluid samples", provides a device that can realize integrated lysis and detection; however, it requires a bottle structure for storing reagent strips, and the overall size is relatively large; at the same time, during the detection process, the liquid sample in the sample absorber needs to be squeezed out, and during this process, a lot of residue is likely to remain in the sample absorber, resulting in waste of liquid specimens. Summary of the invention

[0004] The utility model aims to provide a liquid sample lysis detection device.

[0005] The utility model provides a liquid specimen cracking detection device, which comprises a sampling detection unit, a cracking unit and a detachable limiting structure.

[0006] The sampling detection unit includes a detection housing, a sample collection unit and a detection reagent strip. The detection reagent strip is installed on the detection housing. The detection housing is divided into a gripping section and a plug-in section. An opening is provided at the end of the plug-in section. The sample collection unit is fixed at the opening of the detection housing. The sample collection unit contacts the sample addition area of ​​the detection reagent strip.

[0007] The lysis unit comprises a base, a piercing sheath, a sealing film, and a bottom bottle. The bottom bottle is installed at the bottom of the base and is used to store the lysis solution; the top opening of the bottom bottle is provided with a sealing film. The piercing sheath is slidably connected in the base, and a sharp point is provided at the end facing the bottom bottle.

[0008] The detachable limiting structure is installed between the sampling and detection unit and the lysis unit, and is used to limit the depth of the sampling and detection unit inserted into the top opening of the base in the initial state to avoid piercing the sheath and the sealing film.

[0009] In the detection state, the detachable limiting structure is removed, and the piercing sheath pierces the sealing film under the push of the plug-in section of the detection shell; the sample collection unit extends into the bottom bottle.

[0010] Preferably, the detachable limiting structure is a limiting buckle. The limiting buckle is arranged at the top opening of the base and is integrally formed with the base. The connection between the limiting buckle and the base is a weak structure that can be broken by external force. A transverse limiting rib is arranged on the side of the limiting buckle facing the base. In the initial state, the transverse limiting rib provides a limit to the detection shell of the sampling detection unit.

[0011] Preferably, an elastic convex point is fixed on the inner side wall of the base; the piercing sheath is located between the elastic convex point and the bottom bottle; and a first convex point and a second convex point are provided on the outer side surface of the plug-in section of the detection shell. The first convex point and the second convex point are arranged at intervals along the direction from the plug-in section to the gripping section. The positions of the first convex point and the second convex point match the elastic convex point on the inner side wall of the base. In the initial state, the first convex point on the detection shell passes over the elastic convex point on the inner wall of the base; in the detection state, the second convex point on the detection shell passes over the elastic convex point on the inner wall of the base.

[0012] Preferably, the detachable limiting structure adopts a rod sleeve. The rod sleeve is sleeved on the plug-in section of the detection housing. In the initial state, the top opening of the base of the cracking unit provides a limit for the stepped surface on the rod sleeve.

[0013] Preferably, an elastic convex point is fixed on the inner wall of the base; the piercing sheath is located between the elastic convex point and the bottom bottle; and a second convex point is provided on the outer surface of the plug-in section of the detection housing. The position of the second convex point matches the elastic convex point. In the detection state, the second convex point on the detection housing passes over the elastic convex point on the inner wall of the base.

[0014] Preferably, guiding slopes are provided on both sides of the elastic protrusion.

[0015] Preferably, the detection housing comprises a detection lower plate and a detection upper plate; the opposite sides of the detection lower plate and the detection upper plate are fixed together by multiple groups of plug-in structures. Limiting grooves are provided on both sides of the sample collection unit. The two plug-in structures in the opening of the detection housing are respectively inserted into the limiting grooves on both sides of the sample collection unit.

[0016] Preferably, the plug-in structure comprises a clamping column and a clamping hole which are respectively fixed on the detection upper plate and the detection lower plate and cooperate with each other.

[0017] Preferably, the sample collection unit is made of porous mesh fiber material and is used to absorb and conduct liquid specimens.

[0018] Preferably, an observation window is provided on the detection housing, the result display area of ​​the detection reagent strip is aligned with the observation window panel, and a transparent observation window panel is fixed on the observation window.

[0019] Preferably, there is a sliding resistance between the piercing sheath and the base to prevent the piercing sheath from sliding freely when not subjected to external thrust.

[0020] The utility model has the following beneficial effects:

[0021] 1. In the present invention, when the sample collection unit and the detection reagent strip are integrated into a long strip detection housing, the lysis unit is matched to achieve mixing of the liquid specimen and the lysis solution and delivery of the mixed liquid to the reagent strip by only one pressing action.

[0022] 2. The utility model adopts a pen-type structure with a cover, and does not need a bottle structure for storing reagent strips; while realizing automatic lysis and automatic sample addition, the liquid specimen lysis detection device is further miniaturized, thereby improving the miniaturization degree of the liquid specimen lysis detection device.

[0023] 3. The utility model provides a piercing sheath that can be pushed by the detection shell in the lysis unit, which overcomes the problem that the sample collection unit is not strong enough to pierce the aluminum foil; at the same time, the structure of the piercing sheath being sleeved on the outside of the sample collection unit helps to reduce or even avoid the situation where the sample collection unit is squeezed, bent, and deformed by external force during the process of piercing the aluminum foil, and the liquid specimen on the sample collection unit is prevented from being squeezed out during the lysis process; in conjunction with the process of the sample collection unit sucking the lysis solution upward, the utilization rate of the liquid specimen of the utility model is greatly improved.

[0024] 4. The utility model utilizes a limit buckle or a rod sleeve to prevent the sampling detection unit from being over-inserted into the lysis unit in the initial state, thereby greatly reducing the possibility of damage to the liquid specimen lysis detection device during transportation. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a schematic diagram of the overall structure of Example 1 of the utility model.

[0026] Figure 2 This is a schematic diagram of the explosion of Example 1 of the utility model.

[0027] Figure 3 Schematic diagram of the relative positions of the sample collection unit and the detection reagent strip in Example 1 of the utility model.

[0028] Figure 4 Schematic diagram of the changes before and after the limit buckle is removed from the cracking unit in Example 1 of the utility model

[0029] Figure 5 It is a cross-sectional schematic diagram of the cracking unit in Example 1 of the utility model.

[0030] Figure 6 It is a cross-sectional schematic diagram of the sampling and detection unit in Example 1 of the utility model.

[0031] Figure 7 This is a schematic diagram of the detection process of Example 1 of the present utility model.

[0032] Figure 8 This is a schematic diagram of the overall structure of Example 2 of the utility model.

[0033] Fig. 9 This is a schematic diagram of the explosion of Example 2 of the utility model. DETAILED DESCRIPTION

[0034] The utility model is further described below in conjunction with the accompanying drawings.

[0035] like Figure 1 As shown, a liquid sample lysis detection device includes a sampling detection unit 100 and a lysis unit 200.

[0036] like Figure 2 As shown, the sampling detection unit 100 includes a sample collection unit 105, a detection lower plate 101, a detection upper plate 102, a detection reagent strip 103 and an observation window panel 104. The detection lower plate 101 and the detection upper plate 102 are fixed together on opposite sides by multiple groups of plug-in structures to form a detection housing. The plug-in structure includes a card column and a card hole that are respectively fixed on the detection upper plate 102 and the detection lower plate 101 and cooperate with each other.

[0037] like Figure 2 and 3 As shown, an opening is provided at the bottom of the detection shell. The sample collection unit 105 is fixed at the opening of the detection shell. The inner end of the sample collection unit 105 overlaps with the sample application area of ​​the detection reagent strip 103, so that the sample can be transferred to the detection reagent strip through the sample sampling detection unit. The outer end of the sample collection unit 105 extends to the area outside the detection shell. Limiting grooves are provided on both sides of the sample collection unit 105. The two symmetrical plug-in structures in the opening of the detection shell are respectively inserted into the limiting grooves on both sides of the sample collection unit 105 to achieve fixation between the sample collection unit 105 and the detection shell.

[0038] The sample collection unit 105 is made of porous mesh fiber material, which can absorb the conductive liquid specimen; the observation window panel 104 is made of transparent material; an observation window is opened on the detection upper plate 102. The observation window panel 104 is fixed on the observation window. The detection reagent strip 103 is fixed in the detection housing, and the result display area of ​​the detection reagent strip 103 is aligned with the observation window panel 104.

[0039] like Figure 4 and 5As shown, the lysis unit 200 includes a base 201, a piercing sheath 202, a sealing film 203, a bottom bottle 204 and a limiting buckle 205. The base 201 is hollow, and both the top and bottom ends are open; the bottom bottle 204 is installed at the bottom of the inner cavity of the base 201. The bottom bottle 204 is provided with a lysis solution storage cavity, which can be loaded with lysis solution; the top opening of the bottom bottle 204 is provided with a sealing film 203 that closes the inner cavity of the bottom bottle 204. The piercing sheath 202 is installed in the middle of the inner cavity of the base 201, and is used to slide downward to pierce the sealing film 203. The sealing film 203 is made of aluminum foil.

[0040] like Figure 4 As shown, the limit buckle 205 is set at the top opening of the base 201 and is integrally formed with the base 201. The connection between the limit buckle 205 and the base 201 is a weak structure (lower strength than other areas of the base 201 and the limit buckle 205), which can be broken by external force. The limit buckle 205 is provided with a transverse limit stop rib 206 on the side facing the base 201. The transverse limit stop rib 206 is used to limit the movement of the sampling and detection unit 100 into the inside of the lysis unit 200.

[0041] like Figure 5 and 6 As shown, elastic protrusions 207 are fixed on two opposite side walls of the inner cavity of the base 201; the detection shell composed of the detection lower plate 101 and the detection upper plate 102 is divided into a holding section and a plug-in section. The opening of the detection shell is located at the end of the plug-in section. The first protrusion 106 and the second protrusion 107 are arranged on both outer sides of the plug-in section of the detection shell. The first protrusion 106 and the second protrusion 107 are arranged at intervals along the direction from the plug-in section to the holding section. The positions of the first protrusion 106 and the second protrusion 107 match the elastic protrusion 207 on the inner side wall of the base.

[0042] The elastic protrusion 207 can be deformed under compression. Both sides of the elastic protrusion 207 are provided with guiding inclined surfaces, so that the elastic protrusion 207 can not only constrain the first protrusion 106 and the second protrusion 107, but also allow the first protrusion 106 and the second protrusion 107 to pass over the elastic protrusion 120 when the user pushes or pulls the sampling and detection unit 100; the first protrusion 106 and the second protrusion 107 cooperate with the elastic protrusion 207 to respectively define the relative positions between the sampling and detection unit 100 and the lysis unit 200 in two relative states.

[0043] The piercing sheath 202 is slidably connected in the base 201 and is located between the elastic protrusion 207 and the bottom bottle 204. There is a sliding resistance between the piercing sheath 202 and the base 201 to prevent the piercing sheath 202 from sliding freely when it is not subjected to external thrust. The piercing sheath 202 is hollow, and the sample collection unit 105 passes through the piercing sheath 202; the end of the piercing sheath 202 facing the bottom bottle 204 is provided with a sharp point capable of piercing the sealing film 203.

[0044] The two relative states of the sampling and detection unit 100 and the lysis unit 200 are the initial state and the detection state. In the initial state, the plug section of the detection shell extends into the top opening of the base 201, and the end contacts the end of the piercing sheath 202; the connection between the plug section and the grip section of the detection shell is limited by the limit buckle 205; the first convex point 106 on the detection shell passes over the elastic convex point 207 on the inner wall of the base; at this time, the elastic convex point 207 on the inner wall of the base can limit the first convex point 106 of the sampling and detection unit from moving outward.

[0045] In the detection state, the limit buckle 205 has been broken, the second protrusion 107 on the detection housing has passed the elastic protrusion 207 on the inner wall of the base; the piercing sheath 202 pierces the sealing film 203, and the sample collection unit 105 extends into the bottom bottle 204. At this time, the sampling and detection unit can continue to move toward the lysis unit, and the elastic protrusion 207 on the inner wall of the base can limit the second protrusion 107 of the sampling and detection unit from moving outward.

[0046] The method of using the liquid specimen lysis detection device is as follows:

[0047] Step 1: Pull out the sampling and testing unit 100 from the lysis unit 200 , and use the sample collection unit 105 of the sampling and testing unit 100 to collect the liquid specimen to be tested.

[0048] Step 2: Break the connection between the limit buckle 205 on the cracking unit 200 and the base 201 , and remove the limit buckle 205 .

[0049] Step 3: Figure 7 As shown, the lysis unit 200 is placed vertically; the sampling detection unit 100 is reinserted into the lysis unit 200 and pushed inward, so that the second protrusion 107 on the detection housing passes over the elastic protrusion 207 on the inner wall of the base 201. The piercing sheath 202 pierces the sealing film 203 under the push of the plug-in section of the detection housing, and the sample collection unit 105 extends into the bottom bottle 204.

[0050] Step 4: The sample collection unit 105 absorbs the lysate in the bottom bottle 204 , and the lysate carries the liquid specimen and flows to the sample adding area of ​​the detection reagent strip 103 .

[0051] Step 5: Wait for the test reagent strip 103 to react, and observe the test result on the observation window panel 104 after a preset time.

[0052] Example 2

[0053] like Figure 8 and 9 As shown, a liquid sample lysis detection device, the difference between this embodiment and embodiment 1 is:

[0054] 1) The first protrusion 106 is not provided on the plug-in section of the detection housing; the sampling detection unit further includes a rod sleeve 108. The rod sleeve 108 is sleeved on the outside of the plug-in section of the detection housing and is limited by the second protrusion 107. The rod sleeve 108 is used to protect the sample collection unit. The liquid sample lysis detection device provided in this embodiment removes the rod sleeve 108 before collecting the liquid sample to be tested.

[0055] 2) There is no limit buckle 205 in the lysis unit 200; in the initial state, the stepped surface on the rod sleeve 108 is limited by the end surface of the top opening of the base 201 of the lysis unit to prevent the sampling and detection unit 100 from being over-inserted into the lysis unit 200 and to avoid piercing the sheath 202 and the sealing film 203.

[0056] The method of using the liquid specimen lysis detection device is as follows:

[0057] Step 1: Pull out the sampling and testing unit 100 from the lysis unit 200, remove the rod cover 108, and use the sample collection unit 105 of the sampling and testing unit 100 to collect the liquid specimen to be tested.

[0058] Step 2: Place the lysis unit 200 vertically; reinsert the sampling detection unit 100 without the rod sleeve 108 into the lysis unit 200 and push it inward so that the second protrusion 107 on the detection housing passes over the elastic protrusion 207 on the inner wall of the base 201. The piercing sheath 202 pierces the sealing film 203 under the push of the plug-in section of the detection housing, and the sample collection unit 105 extends into the bottom bottle 204.

[0059] Step 3: The sample collection unit 105 absorbs the lysate in the bottom bottle 204 , and the lysate carries the liquid specimen and flows to the sample adding area of ​​the detection reagent strip 103 .

[0060] Step 4: Wait for the test reagent strip 103 to react, and observe the test result on the observation window panel 104 after a preset time.

Claims

1. A liquid specimen lysis detection device, comprising a sampling detection unit (100), a lysis unit (200) and a detachable limiting structure; characterized in that: The sampling detection unit (100) comprises a detection housing, a sample collection unit (105) and a detection reagent strip (103); the detection reagent strip (103) is mounted on the detection housing; the detection housing is divided into a holding section and a plug-in section; an opening is provided at the end of the plug-in section; the sample collection unit (105) is fixed at the opening of the detection housing; the sample collection unit (105) is in contact with a sample application area of ​​the detection reagent strip (103); The lysis unit (200) comprises a base (201), a piercing sheath (202), a sealing film (203), and a bottom bottle (204); the bottom bottle (204) is installed at the bottom of the base (201) and is used to store lysis solution; the top opening of the bottom bottle (204) is provided with a sealing film (203); the piercing sheath (202) is slidably connected in the base (201), and a sharp point is provided at the end facing the bottom bottle (204); The detachable limiting structure is installed between the sampling and detection unit (100) and the lysis unit (200) and is used to limit the depth of the sampling and detection unit (100) inserted into the top opening of the base (201) in the initial state, so as to avoid piercing the sheath (202) and the sealing film (203); In the detection state, the detachable limiting structure is removed, and the piercing sheath (202) pierces the sealing film (203) under the push of the plug-in section of the detection housing; the sample collection unit (105) extends into the bottom bottle (204).

2. A liquid sample lysis detection device according to claim 1, characterized in that: The detachable limiting structure is a limiting buckle (205); the limiting buckle (205) is arranged at the top opening of the base (201) and is formed integrally with the base (201); the connection between the limiting buckle (205) and the base (201) is a weak structure that can be broken by external force; a transverse limiting rib (206) is arranged on the side of the limiting buckle (205) facing the base (201); in an initial state, the transverse limiting rib (206) provides limiting for the detection shell of the sampling detection unit (100).

3. A liquid sample lysis detection device according to claim 2, characterized in that: An elastic protrusion (207) is fixed on the inner wall of the base (201); the piercing sheath (202) is located between the elastic protrusion (207) and the bottom bottle (204); a first protrusion (106) and a second protrusion (107) are provided on the outer surface of the plug-in section of the detection shell; the first protrusion (106) and the second protrusion (107) are arranged at intervals along the direction from the plug-in section to the gripping section; the positions of the first protrusion (106) and the second protrusion (107) match the elastic protrusion (207) on the inner wall of the base; in the initial state, the first protrusion (106) on the detection shell passes over the elastic protrusion (207) on the inner wall of the base; in the detection state, the second protrusion (107) on the detection shell passes over the elastic protrusion (207) on the inner wall of the base.

4. The liquid sample lysis detection device according to claim 1, characterized in that: The detachable limiting structure adopts a rod sleeve (108); the rod sleeve (108) is sleeved on the plug-in section of the detection shell; in the initial state, the top opening of the base (201) of the cracking unit provides limiting for the stepped surface on the rod sleeve (108).

5. A liquid sample lysis detection device according to claim 4, characterized in that: An elastic protrusion (207) is fixed on the inner wall of the base (201); the piercing sheath (202) is located between the elastic protrusion (207) and the bottom bottle (204); a second protrusion (107) is provided on the outer surface of the plug-in section of the detection shell; the position of the second protrusion (107) matches the elastic protrusion (207); in the detection state, the second protrusion (107) on the detection shell passes over the elastic protrusion (207) on the inner wall of the base.

6. A liquid sample lysis detection device according to claim 3 or 5, characterized in that: Both sides of the elastic protrusion (207) are provided with guiding inclined surfaces.

7. The liquid sample lysis detection device according to claim 1, characterized in that: The detection housing comprises a detection lower plate (101) and a detection upper plate (102); the opposite sides of the detection lower plate (101) and the detection upper plate (102) are fixed together by means of a plurality of groups of plug-in structures; limiting grooves are provided on both sides of the sample collection unit (105); and the two plug-in structures in the opening of the detection housing are respectively inserted into the limiting grooves on both sides of the sample collection unit (105).

8. The liquid sample lysis detection device according to claim 7, characterized in that: The plug-in structure comprises a clamping column and a clamping hole which are respectively fixed on the detection upper plate (102) and the detection lower plate (101) and cooperate with each other.

9. The liquid sample lysis detection device according to claim 1, characterized in that: The sample collection unit (105) is made of porous mesh fiber material and is used to absorb conductive liquid specimens.

10. The liquid sample lysis detection device according to claim 1, characterized in that: There is sliding resistance between the piercing sheath (202) and the base (201) to prevent the piercing sheath (202) from sliding freely when not subject to external thrust.

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