A shaker for fully automatic immunofluorescence staining instrument

Abstract

The utility model belongs to the field of immunofluorescence staining instruments, and provides a shaking table for a fully automatic immunofluorescence staining instrument, including a base plate, a mounting plate, and a cover plate; a first rotating shaft can be rotatably mounted on the base plate, the mounting plate is fixed to the first rotating shaft, a first motor is mounted on the base plate, and the first motor is used to drive the first rotating shaft to rotate; the mounting plate is used to place a culture plate, a second rotating shaft can be rotatably mounted on the mounting plate, the cover plate is adapted to the mounting plate, the cover plate is fixed to the second rotating shaft, a second motor is mounted on the mounting plate, and the second motor is used to drive the second rotating shaft to rotate. The shaking table drives the second rotating shaft to rotate by the second motor, so that the cover plate swings around the second rotating shaft, and the cover plate can be automatically opened and closed. When the cover plate swings to fit with the mounting plate, the culture plate can be covered, which can minimize the volatilization of trace reagent staining and avoid drying of biological samples.

Description

Technical Field

[0001] The utility model belongs to the field of immunofluorescence staining instruments, and in particular relates to a shaking table used for a full-automatic immunofluorescence staining instrument. Background Art

[0002] The fully automatic immunofluorescence staining equipment commonly found on the market is usually used for glass slide staining. There are relatively few equipment for culture plate staining, and most of the equipment for culture plate staining is not fully automatic. The equipment used for culture plate staining needs to integrate the functions of reagent preparation, pipetting, sample addition, mixing, cleaning, incubation, and disinfection to automatically complete the entire staining process. When the existing culture plate staining equipment is incubated on a shaker, the volatilization of volatile reagents and the drying of biological samples often occur during the staining process of trace reagents. It is only suitable for a certain type of cell staining project, with low efficiency and the possibility of misoperation. Utility Model Content

[0003] In view of the shortcomings of the prior art, the utility model provides a shaker for a fully automatic immunofluorescence staining instrument, which can minimize the volatilization of trace reagent staining to the greatest extent by providing a cover plate, thereby preventing the biological sample from drying up.

[0004] In order to achieve the above-mentioned object, the utility model adopts the following technical scheme: a shaking table for a fully automatic immunofluorescence staining instrument, comprising a base plate, a mounting plate, and a cover plate;

[0005] A first rotating shaft is rotatably mounted on the base plate, the mounting plate is fixed to the first rotating shaft, a first motor is mounted on the base plate, and the first motor is used to drive the first rotating shaft to rotate;

[0006] The mounting plate is used for placing the culture plate, a second rotating shaft is rotatably mounted on the mounting plate, the cover plate is matched with the mounting plate, the cover plate is fixed to the second rotating shaft, a second motor is mounted on the mounting plate, and the second motor is used to drive the second rotating shaft to rotate.

[0007] Preferably, a first driving wheel is coaxially fixed to the power output end of the first motor, a first driven wheel is coaxially fixed to the first rotating shaft, and the first driving wheel is connected to the first driven wheel via a first conveyor belt transmission.

[0008] Preferably, a second driving wheel is coaxially fixed to the power output end of the second motor, a second driven wheel is coaxially fixed to the second rotating shaft, and the second driving wheel is connected to the second driven wheel via a second conveyor belt transmission.

[0009] Preferably, a culture plate placement groove is provided on the upper surface of the mounting plate, the culture plate is clamped in the culture plate placement groove, and a plurality of biological sample grooves are provided on the culture plate.

[0010] Preferably, a pick-up and placement groove is provided on the inner side wall of the culture plate placement groove, and the pick-up and placement groove extends to the top surface of the mounting plate.

[0011] Preferably, a plurality of magnets are provided on the bottom surface of the culture plate placement slot.

[0012] Preferably, the plurality of magnets correspond one-to-one to the plurality of biological sample slots.

[0013] Compared with the prior art, the beneficial effects of the utility model are:

[0014] 1. The utility model provides a shaker for a fully automatic immunofluorescence staining instrument. The first motor drives the first rotating shaft to rotate, so that the mounting plate can swing around the first rotating shaft, thereby achieving the purpose of shaking the culture plate in the mounting plate back and forth. The second motor drives the second rotating shaft to rotate, so that the cover plate can swing around the second rotating shaft, thereby achieving automatic opening and closing of the cover plate. When the cover plate swings to fit with the mounting plate, the culture plate can be covered, which can minimize the volatilization of trace reagent staining and prevent the biological sample from drying up.

[0015] 2. The utility model provides a shaker for a fully automatic immunofluorescence staining instrument, in which the setting of the magnet can adsorb and fix the cells bound to the immune magnetic beads on the bottom of the biological sample slot. When shaking, the cells bound to the immune magnetic beads will not run to the edge of the biological sample slot. In this way, when the waste liquid is sucked from the edge of the biological sample slot after staining, the cells bound to the immune magnetic beads will not be sucked away. At the same time, it can also ensure that the staining reagent is in full contact with the cells, thereby achieving a better staining effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 A schematic diagram of the three-dimensional structure of a shaker for a fully automatic immunofluorescence staining instrument provided in an embodiment of the utility model;

[0017] Figure 2 A schematic diagram of the three-dimensional structure of a shaker for a fully automatic immunofluorescence staining instrument provided in an embodiment of the utility model;

[0018] Figure 3 A schematic diagram of the top view structure of a mounting plate and related parts of a shaker for a fully automatic immunofluorescence staining instrument provided in an embodiment of the utility model.

[0019] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0020] 1. Base plate; 2. Mounting plate; 3. Cover plate; 4. First motor; 5. First rotating shaft; 6. Second rotating shaft; 7. Second motor; 8. Culture plate placement slot; 9. Culture plate; 10. Pick-and-place slot; 11. Magnet; 12. Biological sample slot. DETAILED DESCRIPTION

[0021] The present invention will be further described in detail below in conjunction with specific embodiments so that those skilled in the art can understand the present invention more clearly.

[0022] It should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integrally formed structure. For ordinary technicians in this field, the specific meanings of such terms in this patent can be understood according to specific circumstances.

[0023] like Figure 1-3 As shown, this embodiment provides a shaker for a fully automatic immunofluorescence staining instrument, including a base plate 1, a mounting plate 2, and a cover plate 3;

[0024] A first rotating shaft 5 is rotatably mounted on the base plate 1 through a bearing, the first rotating shaft 5 is located above the base plate 1, the mounting plate 2 is located above the first rotating shaft 5, the first rotating shaft 5 is parallel to the long side of the mounting plate 2, the mounting plate 2 is fixedly connected to the first rotating shaft 5 through a first connecting rod, and a first motor 4 is installed at the bottom of the base plate 1, the first motor 4 is used to drive the first rotating shaft 5 to rotate;

[0025] The mounting plate 2 is used for placing a culture plate 9, and the culture plate 9 is used for placing biological samples.

[0026] A second rotating shaft 6 is rotatably mounted on the mounting plate 2 via a bearing, the second rotating shaft 6 is located on one side of the long side of the mounting plate 2, the second rotating shaft 6 is parallel to the long side of the mounting plate 2, the cover plate 3 is adapted to the mounting plate 2, the cover plate 3 is fixed to the second rotating shaft 6 via a second connecting rod, the cover plate 3 can be swung to cover the mounting plate 2, a second motor 7 is mounted on the mounting plate 2, the second motor 7 is used to drive the second rotating shaft 6 to rotate.

[0027] Based on the above structure, the first motor 4 drives the first rotating shaft 5 to rotate, so that the mounting plate 2 can swing around the first rotating shaft 5, thereby achieving the purpose of rocking the culture plate 9 back and forth. The second motor 7 drives the second rotating shaft 6 to rotate, so that the cover plate 3 can swing around the second rotating shaft 6, thereby achieving automatic opening and closing of the cover plate 3. When the cover plate 3 swings to fit the mounting plate 2, the culture plate 9 can be covered, which can minimize the volatilization of trace reagent dye and prevent the biological sample from drying up.

[0028] Please refer again Figure 1 In this embodiment, a first driving wheel is coaxially fixed to the power output end of the first motor 4, a first driven wheel is coaxially fixed to the first rotating shaft 5, and the first driving wheel is connected to the first driven wheel through a first conveyor belt.

[0029] The first driving wheel is driven to rotate by the first motor 4, and then the first driven wheel is driven to rotate by the first conveyor belt, thereby driving the first rotating shaft 5 to rotate. The first motor 4 has a built-in brake, which can increase the torque of the motor to prevent the rocking bed from tipping over due to insufficient torque of the motor when the cover is opened.

[0030] A second driving wheel is coaxially fixed to the power output end of the second motor 7, a second driven wheel is coaxially fixed to the second rotating shaft 6, and the second driving wheel is connected to the second driven wheel through a second conveyor belt transmission.

[0031] The second driving wheel is driven to rotate by the second motor 7 , and then the second driven wheel is driven to rotate by the second conveyor belt, thereby driving the second rotating shaft 6 to rotate.

[0032] In this embodiment, two culture plate placement slots 8 are provided on the upper surface of the mounting plate 2, and the culture plate 9 is clamped in the culture plate placement slots 8. The culture plate 9 is provided with six biological sample slots 12 for placing biological samples.

[0033] That is to say, one culture plate 9 can hold six biological samples at the same time, and two culture plate placement slots 8 can hold two culture plates 9 .

[0034] A pick-up and placement groove 10 is provided on the inner side wall of the culture plate placement groove 8 , and the pick-up and placement groove 10 extends to the top surface of the mounting plate 2 .

[0035] The provision of the taking and placing groove 10 facilitates manual taking out of the culture plate 9 .

[0036] In this embodiment, six magnets 11 are provided on the inner bottom surface of the culture plate placement slot 8. The six magnets 11 correspond to the six biological sample slots 12 one by one.

[0037] The setting of the magnet 11 can adsorb and fix the cells bound to the immune magnetic beads on the bottom of the biological sample groove 12, so that when shaking, the cells bound to the immune magnetic beads will not run to the edge of the biological sample groove 12. In this way, when the waste liquid is sucked from the edge of the biological sample groove 12 after staining, the cells bound to the immune magnetic beads will not be sucked away, and at the same time, it can ensure that the staining reagent is in full contact with the cells, thereby achieving a better staining effect.

[0038] The mechanisms, components and parts not specifically described in the present invention are all existing structures in the prior art and can be directly purchased from the market.

[0039] In the description of the present utility model, it should be understood that the terms "upper", "lower", "left", "right", etc. indicating directions or positional relationships are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, and a specific direction structure and operation, and therefore, cannot be understood as a limitation on the present utility model. In addition, "first" and "second" are only for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present utility model, unless otherwise specified, "multiple" means two or more.

[0040] The above is only a preferred embodiment of the utility model, and is not intended to limit the protection scope of the utility model. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A shaker for a fully automatic immunofluorescence staining instrument, characterized in that: It comprises a base plate (1), a mounting plate (2) and a cover plate (3); A first rotating shaft (5) is rotatably mounted on the base plate (1), the mounting plate (2) is fixed to the first rotating shaft (5), and a first motor (4) is mounted on the base plate (1), the first motor (4) being used to drive the first rotating shaft (5) to rotate; The mounting plate (2) is used for placing a culture plate (9); a second rotating shaft (6) is rotatably mounted on the mounting plate (2); the cover plate (3) is matched with the mounting plate (2); the cover plate (3) is fixed to the second rotating shaft (6); a second motor (7) is mounted on the mounting plate (2); and the second motor (7) is used for driving the second rotating shaft (6) to rotate.

2. A shaker for a fully automatic immunofluorescence staining instrument according to claim 1, characterized in that: A first driving wheel is coaxially fixed to the power output end of the first motor (4), and a first driven wheel is coaxially fixed to the first rotating shaft (5). The first driving wheel and the first driven wheel are connected through a first conveyor belt transmission.

3. A shaker for a fully automatic immunofluorescence staining instrument according to claim 1, characterized in that: A second driving wheel is coaxially fixed to the power output end of the second motor (7), a second driven wheel is coaxially fixed to the second rotating shaft (6), and the second driving wheel is connected to the second driven wheel through a second conveyor belt.

4. A shaker for a fully automatic immunofluorescence staining instrument according to claim 1, characterized in that: The upper surface of the mounting plate (2) is provided with a culture plate placement groove (8), the culture plate (9) is clamped in the culture plate placement groove (8), and the culture plate (9) is provided with a plurality of biological sample grooves (12).

5. A shaker for a fully automatic immunofluorescence staining instrument according to claim 4, characterized in that: A taking-in and putting groove (10) is provided on the inner side wall of the culture plate placement groove (8), and the taking-in and putting groove (10) extends to the top surface of the mounting plate (2).

6. A shaker for a fully automatic immunofluorescence staining instrument according to claim 4, characterized in that: The inner bottom surface of the culture plate placement slot (8) is provided with a plurality of magnets (11).

7. A shaker for a fully automatic immunofluorescence staining instrument according to claim 6, characterized in that: The plurality of magnets (11) correspond one-to-one to the plurality of biological sample slots (12).

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