A mixed material fermentation device for producing Agaricus bisporus strains

Abstract

The utility model provides a mixed fermentation device for producing Agaricus bisporus strains, which relates to the technical field of edible fungus cultivation, and comprises a base frame, two support rods fixedly installed on the top of the base frame, a mixing tank fixedly installed between the two support rods, a motor fixedly installed on the top of the mixing tank, a feeding nozzle arranged on the top of the mixing tank, an auger fixedly installed on the back of the mixing tank, a discharge hopper arranged on the lower end of the auger, a feeding pipe fixedly installed on the side of the auger close to the mixing tank, and the feeding pipe corresponds to the feeding nozzle. In the utility model, the survival coverage rate of the prepared fermentation material for culturing mushrooms is the highest, and Agaricus bisporus can be preferentially prevented from being invaded by insect diseases, which solves the problem of pathogens and pests in the fermentation material in the prior art, solves the problem of low nutrition of the fermentation material and low coverage rate of the cultivation of mushrooms, and is convenient for purifying and propagating Agaricus bisporus strains, while improving the quality and yield of Agaricus bisporus.

Description

Technical Field

[0001] The utility model relates to the technical field of edible fungus cultivation, in particular to a mixed material fermentation device for producing Agaricus bisporus strains. Background Art

[0002] The fruiting body of Agaricus bisporus is medium-sized, hemispherical at first, then flat, white, slightly dry and gradually turning yellow, with the edges curled inward at first. It is called foreign mushroom and white mushroom. It is one of my country's traditional cultivated and exported edible fungi. Agaricus bisporus has many functions and effects, so it is very common in life and has become the edible fungus with the largest cultivation area and the highest yield in the world.

[0003] At present, the production and market supply of Agaricus bisporus as an edible fungus has greatly enriched people's material life. Since Agaricus bisporus is a regenerative fungus, it relies on nutrients in the attached feces grass for growth. Therefore, the culture medium necessary for the growth of Agaricus bisporus has become the main research topic in recent years. Due to the growth environment of Agaricus bisporus, it is impossible to control the nutrients in it and sterilize and inhibit bacteria. Therefore, Agaricus bisporus is easily attacked by pests, resulting in slow growth or disease problems.

[0004] Based on the above, the inventors of the present invention discovered the following problems: in the existing technology, the fermentation material is often invaded by pathogens and pests, which not only reduces the quality of the fermentation material, but also affects the cultivation effect of Agaricus bisporus due to its insufficient nutritional value, resulting in a low cultivation coverage rate; these problems further increase the difficulty of later purification and propagation of Agaricus bisporus strains.

[0005] Therefore, in view of this, the existing structure and defects are studied and improved, and a mixed fermentation device for making Agaricus bisporus strains is provided, in order to achieve the purpose of having more practical value. Utility Model Content

[0006] 1. Technical issues to be resolved

[0007] In view of the deficiencies in the prior art, the utility model provides a mixed material fermentation device for producing Agaricus bisporus strains, which solves the problems mentioned in the above background technology.

[0008] (II) Technical solution

[0009] To achieve the above purpose, the utility model is implemented through the following technical scheme: a mixed fermentation device for making Agaricus bisporus spawn, comprising a base frame, two support rods fixedly installed on the top of the base frame, a mixing tank fixedly installed between the two support rods, a motor fixedly installed on the top of the mixing tank, a feeding nozzle arranged on the top of the mixing tank, an auger fixedly installed on the back of the mixing tank, a discharge hopper arranged on the lower end of the auger, a feeding pipe fixedly installed on the side of the auger close to the mixing tank, the feeding pipe corresponds to the feeding nozzle, a discharge nozzle is arranged at the bottom of the mixing tank, a baffle is movably installed on the inner side of the discharge nozzle, electric telescopic rods are fixedly installed on both the front and rear sides of the discharge nozzle, the electric telescopic rods are fixedly connected to the baffle, and a filter disc is fixedly installed on the inner side of the mixing tank;

[0010] The output end of the motor is provided with a rotating shaft, and a crushing knife and a scraper are respectively fixedly provided on the outer side of the rotating shaft, wherein the crushing knife is located above the scraper, and the scraper contacts and slides with the filter disc;

[0011] A stirring rod and a scraping rod are fixedly mounted on the outer side of the rotating shaft, respectively. The stirring rod is located above the scraping rod, and the scraping rod is in sliding contact with the inner bottom wall of the mixing tank.

[0012] A support frame is fixedly installed on the top of the base frame, a collecting trough is arranged above the support frame, handles are fixedly installed on the left and right sides of the collecting trough, the collecting trough corresponds to the discharge nozzle, and the stirring rod is located below the filter plate.

[0013] In order to prepare fermentation material culture strains, a mixed fermentation device for preparing Agaricus bisporus strains of the utility model includes the following fermentation raw materials of weight components prepared and mixed for use:

[0014] Main ingredients: 10-15 parts of cow dung, 20-30 parts of wheat straw;

[0015] Auxiliary materials: 15-25 parts of wheat bran, 6-9 parts of superphosphate, 4-8 parts of lime powder, 2-6 parts of gypsum powder, 3-4 parts of urea, 10-15 parts of edible fungus three-dimensional nutritional essence;

[0016] Step 1: crush the raw materials, put the main materials and auxiliary materials into the discharge hopper, start the auger to automatically transport the raw materials to the feeding pipe, connect the feeding pipe with the feeding nozzle, and put the raw materials into the mixing tank for crushing. The crushing length is about 1 cm;

[0017] Step 2: Mixing the raw materials, the main material and the auxiliary material crushed in step 1 are screened through a filter disc in a mixing tank and then mixed;

[0018] Step 3: Mixing and fermenting, the mixed raw materials in step 2 are discharged into a collecting tank through a discharge nozzle at the bottom of the mixing tank through an electric telescopic rod extending to drive the baffle to move, and the mixed raw materials are collected, water is added to the collecting tank and soaked for 2-4 days, and the mixed raw materials after soaking are piled and fermented in a ventilated place, the temperature of the fermentation process is controlled at 23° C.-25° C., and the humidity is maintained at 65-70%. After the fermentation is completed, a fermentation material for Agaricus bisporus production is obtained;

[0019] Step 4: Cultivation of fungus strains. Evenly spread the fermentation material prepared in step 3 on the sterilized mushroom bed. The material layer is 20 cm thick. Rub the fungus strains and spread them evenly. Keep the temperature at 25℃-27.5℃. After 16 days of growth, you will get the mushroom strains.

[0020] The purification and propagation preparation technology for the Agaricus bisporus strains described above has the following steps:

[0021] S1. Bacteria purification:

[0022] Selection of seed mushrooms: Select single mushrooms that grow normally, produce mushrooms evenly, are free of pests and diseases, have a round shape, are white, have thick caps, and thick stems as seed mushrooms;

[0023] Spore isolation: Spores were collected using sterile filter paper and isolated under sterile conditions;

[0024] S2. Bacteria propagation:

[0025] Prepare the culture medium: sterilize the PDA culture medium tube, provide sufficient nutrients to the PDA culture medium tube, and maintain a suitable growth environment;

[0026] Inoculation: Use a sterile syringe to draw up the spore solution and inoculate it into a PDA medium test tube;

[0027] Mycelium screening: select robust mycelium for propagation;

[0028] S3. The above-mentioned S1 and S2 operating environments are carried out according to methods known in the art.

[0029] (III) Beneficial effects

[0030] The utility model provides a mixed material fermentation device for producing Agaricus bisporus strains. The beneficial effects are as follows:

[0031] In the utility model, the survival coverage rate of the prepared fermentation material for cultivation is the highest, and the Agaricus bisporus can be preferentially prevented from being attacked by insects and diseases. The purification and propagation technology improves the purity of the Agaricus bisporus strains and increases the yield of the strains in the actual application of the Agaricus bisporus strain preparation. Through actual production application, it plays an important role in improving the cultivation quality and reducing the incidence of diseases and insect pests, and reduces the difficulty of purification and propagation of the Agaricus bisporus strains in the later stage, thereby solving the problem of pathogens and pests infestation in the fermentation material in the prior art, solving the problem of low nutrition of the fermentation material and low coverage rate of the cultivation of the mushroom seeds, facilitating the purification and propagation of the Agaricus bisporus strains, and improving the quality and yield of the Agaricus bisporus fungi. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 This is a schematic diagram of the mixing structure of the utility model;

[0033] Figure 2 It is a schematic diagram of a partial mixing section of the utility model.

[0034] In the figure: 1. base frame; 2. support frame; 3. support rod; 4. mixing tank; 5. motor; 501. rotating shaft; 502. crushing knife; 503. scraper; 504. stirring rod; 505. scraper rod; 6. discharge nozzle; 7. baffle; 8. collecting trough; 9. handle; 10. feeding pipe; 11. filter plate; 12. auger; 13. discharge hopper; 14. electric telescopic rod. DETAILED DESCRIPTION

[0035] The utility model is further described in detail below through the drawings and embodiments.

[0036] Embodiment 1:

[0037] The utility model embodiment provides a mixed material fermentation device for producing Agaricus bisporus strains, such as Figure 1-2 As shown, it includes a base frame 1, two support rods 3 fixedly installed on the top of the base frame 1, a mixing tank 4 is fixedly installed between the two support rods 3, a motor 5 is fixedly installed on the top of the mixing tank 4, a feeding nozzle is arranged on the top of the mixing tank 4, an auger 12 is fixedly installed on the back of the mixing tank 4, a discharge hopper 13 is arranged at the lower end of the auger 12, a feeding pipe 10 is fixedly installed on the side of the auger 12 close to the mixing tank 4, the feeding pipe 10 corresponds to the feeding nozzle, a discharge nozzle 6 is arranged at the bottom of the mixing tank 4, a baffle 7 is movably installed on the inner side of the discharge nozzle 6, electric telescopic rods 14 are fixedly installed on both the front and rear sides of the discharge nozzle 6, the electric telescopic rod 14 is fixedly connected to the baffle 7, and a filter disc 11 is fixedly installed on the inner side of the mixing tank 4;

[0038] The output end of the motor 5 is provided with a rotating shaft 501, and a crushing knife 502 and a scraper 503 are respectively fixedly installed on the outer side of the rotating shaft 501. The crushing knife 502 is located above the scraper 503, and the scraper 503 contacts and slides with the filter disc 11;

[0039] A stirring rod 504 and a scraping rod 505 are fixedly mounted on the outer side of the rotating shaft 501. The stirring rod 504 is located above the scraping rod 505. The scraping rod 505 is in sliding contact with the inner bottom wall of the mixing tank 4.

[0040] A support frame 2 is fixedly installed on the top of the base frame 1, a collecting tank 8 is arranged above the support frame 2, handles 9 are fixedly installed on the left and right sides of the collecting tank 8, the collecting tank 8 corresponds to the discharge nozzle 6, and the stirring rod 504 is located below the filter plate 11.

[0041] In this embodiment, the motor 5 drives the rotating shaft 501 to rotate and drives the corresponding crushing knife 502 and scraper 503 to crush the fermentation raw materials. The qualified fermentation raw materials will fall through the filter plate 11, and the falling fermentation raw materials will be stirred and mixed by the stirring rod 504 and the scraper rod 505 to mix the fermentation raw materials cultivated with Agaricus bisporus strains. The electric telescopic rod 14 is extended to drive the baffle 7 to move, and the mixed fermentation raw materials in the mixing tank 4 are discharged into the collecting tank 8 through the discharge nozzle 6 for fermentation and cultivation of mushroom species.

[0042] Embodiment 2:

[0043] Experiment 1

[0044] A mixed material fermentation device for producing Agaricus bisporus strains, comprising fermentation raw materials of the following weight components prepared and mixed for use:

[0045] Main ingredients: 10 parts of cow dung, 20 parts of wheat straw;

[0046] Auxiliary materials: 15 parts of wheat bran, 6 parts of superphosphate, 4 parts of lime powder, 2 parts of gypsum powder, 3 parts of urea, 10 parts of edible fungus three-dimensional nutrient essence;

[0047] Step 1: crush the raw materials, put the main materials and auxiliary materials into the discharge hopper 13, and automatically transport the raw materials to the feeding pipe 10 by starting the auger 12. Through the docking of the feeding pipe 10 and the feeding nozzle, the raw materials are put into the mixing tank 4 for crushing, and the crushing length is about 1 cm;

[0048] Step 2: Mixing the raw materials, the main material and the auxiliary material crushed in step 1 are screened in the mixing tank 4 through the filter disc 11 and then mixed;

[0049] Step 3: Mixing and fermenting, the mixed raw materials in step 2 are discharged into a collecting tank 8 through a discharge nozzle 6 at the bottom of a mixing tank 4 through an electric telescopic rod 14 extending to drive a baffle 7 to move, and the mixed raw materials are collected, and water is added to the collecting tank 8 and soaked for 2 days. The mixed raw materials after soaking are piled and fermented in a ventilated place, and the temperature of the fermentation process is controlled at 23° C. and the humidity is maintained at 65%. After the fermentation is completed, a fermentation material for Agaricus bisporus production is obtained;

[0050] Step 4: Cultivation of fungus strains. Evenly spread the fermentation material prepared in step 3 on the sterilized mushroom bed. The material layer should be 20 cm thick. Rub the fungus strains and spread them evenly. Keep the temperature at 25°C. After 16 days of growth, you will get the mushroom strains.

[0051] Experiment 2

[0052] A mixed material fermentation device for producing Agaricus bisporus strains, comprising fermentation raw materials of the following weight components prepared and mixed for use:

[0053] Main ingredients: 12 parts of cow dung, 25 parts of wheat straw;

[0054] Auxiliary materials: 20 parts of wheat bran, 7 parts of superphosphate, 6 parts of lime powder, 5 parts of gypsum powder, 3 parts of urea, 12 parts of edible fungus three-dimensional nutrient essence;

[0055] Step 1: crush the raw materials, put the main materials and auxiliary materials into the discharge hopper 13, and automatically transport the raw materials to the feeding pipe 10 by starting the auger 12. Through the docking of the feeding pipe 10 and the feeding nozzle, the raw materials are put into the mixing tank 4 for crushing, and the crushing length is about 1 cm;

[0056] Step 2: Mixing the raw materials, the main material and the auxiliary material crushed in step 1 are screened in the mixing tank 4 through the filter disc 11 and then mixed;

[0057] Step 3: Mixing and fermenting, the mixed raw materials in step 2 are discharged into a collecting tank 8 through a discharge nozzle 6 at the bottom of a mixing tank 4 through an electric telescopic rod 14 extending to drive a baffle 7 to move, and the mixed raw materials are collected, and water is added to the collecting tank 8 and soaked for 3 days. The mixed raw materials after soaking are piled and fermented in a ventilated place, and the temperature of the fermentation process is controlled at 24° C. and the humidity is maintained at 68%. After the fermentation is completed, a fermentation material for Agaricus bisporus production is obtained;

[0058] Step 4: Cultivation of fungus strains. Evenly spread the fermentation material prepared in step 3 on the sterilized mushroom bed. The material layer should be 20 cm thick. Rub the fungus strains and spread them evenly. Keep the temperature at 26°C. After 16 days of growth, you will get the mushroom strains.

[0059] Experiment 3

[0060] A mixed material fermentation device for producing Agaricus bisporus strains, comprising fermentation raw materials of the following weight components prepared and mixed for use:

[0061] Main ingredients: 15 parts of cow dung, 30 parts of wheat straw;

[0062] Auxiliary materials: 25 parts of wheat bran, 9 parts of superphosphate, 8 parts of lime powder, 6 parts of gypsum powder, 4 parts of urea, 15 parts of edible fungus three-dimensional nutrient essence;

[0063] Step 1: crush the raw materials, put the main materials and auxiliary materials into the discharge hopper 13, and automatically transport the raw materials to the feeding pipe 10 by starting the auger 12. Through the docking of the feeding pipe 10 and the feeding nozzle, the raw materials are put into the mixing tank 4 for crushing, and the crushing length is about 1 cm;

[0064] Step 2: Mixing the raw materials, the main material and the auxiliary material crushed in step 1 are screened in the mixing tank 4 through the filter disc 11 and then mixed;

[0065] Step 3: Mixing and fermenting, the mixed raw materials in step 2 are discharged into a collecting tank 8 through a discharge nozzle 6 at the bottom of a mixing tank 4 through an electric telescopic rod 14 extending to drive a baffle 7 to move, and the mixed raw materials are collected, and water is added to the collecting tank 8 and soaked for 4 days. The mixed raw materials after soaking are piled and fermented in a ventilated place, and the temperature of the fermentation process is controlled at 25° C. and the humidity is maintained at 70%. After the fermentation is completed, a fermentation material for Agaricus bisporus production is obtained;

[0066] Step 4: Cultivation of fungus strains. Evenly spread the fermentation material prepared in step 3 on the sterilized mushroom bed. The material layer is 20 cm thick. Rub the fungus strains and spread them evenly. Keep the temperature at 27.5°C. After 16 days of growth, you will get the mushroom strains.

[0067] In summary:

[0068] Cow dung: rich in carbon and minerals, it is the main source of nitrogen needed for the growth of Agaricus bisporus.

[0069] Wheat straw: rich in carbon, it is the main source of carbon for the growth of Agaricus bisporus.

[0070] Wheat bran: provides additional carbon source and organic nutrients.

[0071] Superphosphate: Contains 15% to 20% effective phosphoric acid, which can supplement the deficiency of phosphorus and calcium, promote the decomposition activity of microorganisms, and is beneficial to the fermentation and maturity of the pile.

[0072] Lime powder: used to adjust the pH value of the culture medium and kill potential pathogens and pests.

[0073] Gypsum powder: provides calcium, which helps the growth of Agaricus bisporus.

[0074] Urea: Provides an additional source of nitrogen.

[0075] Edible Fungi Three-Dimensional Nutrition Essence: Provides comprehensive nutritional support to promote the growth and development of Agaricus bisporus.

[0076] The experimental results are as follows:

[0077] Experimental batch Experiment 1 Experiment 2 Experiment 3 Mushroom coverage rate (%) 82％ 95％ 88％

[0078] By comparing the data of Experiment 1, Experiment 2, and Experiment 3, it was found that the survival coverage rate of the mushroom seeds cultivated with the fermentation material prepared according to Experiment 2 was the highest.

[0079] Embodiment three:

[0080] A purification and propagation preparation technology for Agaricus bisporus strains, the preparation technology steps are as follows:

[0081] S1. Bacteria purification:

[0082] Selection of seed mushrooms: Select single mushrooms that grow normally, produce mushrooms evenly, are free of pests and diseases, have a round shape, are white, have thick caps, and thick stems as seed mushrooms;

[0083] Spore isolation: Spores were collected using sterile filter paper and isolated under sterile conditions;

[0084] S2. Bacteria propagation:

[0085] Prepare the culture medium: sterilize the PDA culture medium tube, provide sufficient nutrients to the PDA culture medium tube, and maintain a suitable growth environment;

[0086] Inoculation: Use a sterile syringe to draw up the spore solution and inoculate it into a PDA medium test tube;

[0087] Mycelium screening: select robust mycelium for propagation;

[0088] S3. The above-mentioned S1 and S2 operating environments are carried out according to methods known in the art.

[0089] Practical application: The actual application of purification and propagation technology in the preparation of Agaricus bisporus strains: improving the purity of strains and increasing strain yield.

[0090] Effect evaluation: Through actual production application, it has played an important role in improving cultivation quality and reducing the incidence of diseases and pests.

[0091] The standard parts used in the utility model can be purchased from the market, and special-shaped parts can be customized according to the description in the specification and the drawings. The specific connection methods of various parts adopt the conventional means such as mature rivets and welding in the prior art. Machinery, parts and equipment all adopt conventional models in the prior art, and the circuit connection adopts the conventional connection method in the prior art, which will not be described in detail here. The contents not described in detail in this specification belong to the prior art known to professional and technical personnel in this field. All electrical equipment in the utility model are powered by an external power supply.

[0092] In the description of the present invention, it should be noted that the terms "vertical", "up", "down", "horizontal", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0093] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0094] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A mixed material fermentation device for producing Agaricus bisporus strains, characterized in that: The invention comprises a base frame (1), two support rods (3) fixedly mounted on the top of the base frame (1), a mixing tank (4) fixedly mounted between the two support rods (3), a motor (5) fixedly mounted on the top of the mixing tank (4), a feed nozzle arranged on the top of the mixing tank (4), an auger (12) fixedly mounted on the rear of the mixing tank (4), a discharge hopper (13) arranged at the lower end of the auger (12), and a material discharging hopper (13) arranged near the auger (12) near the mixing tank. A feeding pipe (10) is fixedly installed on one side of the mixing tank (4), and the feeding pipe (10) corresponds to the feeding nozzle. A discharge nozzle (6) is arranged at the bottom of the mixing tank (4), and a baffle (7) is movably installed inside the discharge nozzle (6). Electric telescopic rods (14) are fixedly installed on both the front and rear sides of the discharge nozzle (6), and the electric telescopic rods (14) are fixedly connected to the baffle (7). A filter disc (11) is fixedly installed inside the mixing tank (4); A rotating shaft (501) is installed at the output end of the motor (5), and a crushing knife (502) and a scraper (503) are fixedly installed on the outer side of the rotating shaft (501), the crushing knife (502) is located above the scraper (503), and the scraper (503) contacts and slides with the filter disc (11); A stirring rod (504) and a scraping rod (505) are fixedly mounted on the outer side of the rotating shaft (501), the stirring rod (504) is located above the scraping rod (505), and the scraping rod (505) is in sliding contact with the inner bottom wall of the mixing tank (4); A support frame (2) is fixedly mounted on the top of the base frame (1), a collecting trough (8) is arranged above the support frame (2), handles (9) are fixedly mounted on both the left and right sides of the collecting trough (8), the collecting trough (8) corresponds to the discharge nozzle (6), and the stirring rod (504) is located below the filter plate (11).

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