Hirsutella sinensis culture medium preparation device

Abstract

The utility model relates to a hirsutella sinensis culture medium preparation device in the technical field of culture medium preparation, which comprises a machine frame, a preparation mechanism and a cleaning mechanism are arranged in a cabinet body of the machine frame, one side of a table top of the machine frame is provided with an object placing table, and the other side of the table top of the machine frame is provided with a split charging mechanism and a conveying mechanism; the preparation mechanism is communicated with the sub-packaging mechanism and is used for conveying prepared culture medium liquid to the sub-packaging mechanism; the conveying mechanism is arranged below the subpackaging mechanism and used for intermittently delivering the cultivation bottles to be filled in a running water mode. According to the hirsutella sinensis culture medium preparation device, the steps of preparation, pH adjustment, boiling, stirring, split charging and the like of hirsutella sinensis culture medium preparation are integrated and processed, and the hirsutella sinensis culture medium preparation device is suitable for batch line production.

Description

Technical Field

[0001] The utility model relates to a device for preparing a culture medium of Hirsutella chinensis, and belongs to the technical field of culture medium preparation. Background Art

[0002] The Cordyceps sinensis referred to in traditional Chinese medicine is a symbiotic insect-fungus complex formed by the larvae of the bat moth family and the Chinese Hirsutella chinensis. The asexual form of the Cordyceps sinensis fungus is Chinese Hirsutella chinensis. The culture medium used for the cultivation of Chinese Hirsutella chinensis, also known as liquid culture medium, is a liquid culture medium for microorganisms or animal and plant cells, as opposed to solid culture medium. It is an artificially prepared nutrient for the growth and maintenance of microorganisms, plants and animal tissues, usually containing carbohydrates, nitrogen-containing substances, inorganic salts (including trace elements), vitamins and water, etc.

[0003] The steps of preparing liquid culture medium usually include batching, dissolving, adjusting pH, packaging, wrapping, sterilization and storage, etc. In the prior art, the relevant technical field also discloses a Chinese patent CN219091937U culture medium preparation and packaging integrated equipment, which discloses an integrated equipment that can be used for liquid culture medium preparation and packaging, but the equipment disclosed therein cannot realize flow-type operation; and after completing a batch of packaging, the kettle container must be removed from the equipment for cleaning and refilling with water for liquid culture medium preparation, which has low operation efficiency and is not suitable for batch production. Utility Model Content

[0004] In order to solve the above problems existing in the prior art, the utility model provides a device for preparing a culture medium of Hirsutella sinensis.

[0005] The technical solution of the utility model is as follows:

[0006] A device for preparing a culture medium of Hirsutella sinensis comprises a frame, a preparation mechanism and a cleaning mechanism are arranged in a cabinet of the frame, one side of the table of the frame is a storage table, and the other side is provided with a subpackaging mechanism and a conveying mechanism;

[0007] The preparation mechanism is connected with the packaging mechanism and delivers the prepared culture medium liquid to the packaging mechanism;

[0008] The conveying mechanism is arranged below the dispensing mechanism and is used for intermittently delivering the cultivation bottles to be filled in a flowing manner;

[0009] The cleaning mechanism includes a water tank, a pressure pump, a water pipe and a pressure water gun. The input end of the pressure pump is connected to the water tank for extracting cleaning water. The output end of the pressure pump is connected to the pressure water gun through the water pipe for delivering high-pressure cleaning water. The pressure water gun is mounted above the rack table and a spring telescopic hose is provided at the tail end.

[0010] It also includes a PLC controller for controlling the preparation mechanism, cleaning mechanism, packaging mechanism and conveying mechanism.

[0011] Among them, a water injection pipe connected to an external water source is arranged on the top of the water storage tank, a water injection valve is arranged on the water injection pipe, a water level sensor is arranged at a preset maximum liquid level on the inner wall of the water storage tank, and the water injection valve and water level sensor are electrically connected to the PLC controller respectively.

[0012] Wherein, the preparation mechanism includes a preparation kettle for storing culture medium liquid, a power agitator is arranged at the top of the inner cavity of the preparation kettle, a temperature control device is arranged at the bottom of the preparation kettle, a delivery hose is arranged in the preparation kettle, and the delivery hose is connected to the filling mechanism.

[0013] Wherein, a pH reagent kit is arranged on the top of the preparation kettle, and a pH sensor is arranged in the inner cavity of the preparation kettle.

[0014] The dispensing mechanism comprises a dispensing machine body, a dispensing pipe is arranged at the front end of the dispensing machine body, the dispensing pipe is cantilevered and located above the conveying mechanism.

[0015] A ceiling is arranged on the top of the frame, a control panel electrically connected to a PLC controller is embedded on the table top of the frame, a double-door cabinet is arranged under the frame, and universal wheels are arranged at the bottom of the frame.

[0016] The utility model has the following beneficial effects:

[0017] The utility model discloses a device for preparing a culture medium for Hirsutella spp., which is integrated with a preparation mechanism, a cleaning mechanism, a filling mechanism and a conveying mechanism. In particular, the provision of the conveying mechanism and the cleaning mechanism can realize online cleaning and water injection of a flowing intermittent delivery container and a preparation kettle, thereby improving the efficiency and convenience of culture medium preparation. The utility model realizes integration of the steps of preparation, pH adjustment, boiling, stirring and filling of the culture medium for Hirsutella spp. into an integrated and streamlined manner, thus being suitable for batch flow operations. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0019] Figure 2 This is a schematic diagram of the internal structure of the utility model from the side;

[0020] Figure 3 This is a schematic diagram of the internal structure of the utility model from the front;

[0021] Figure 4 This is a schematic diagram of the PLC control connection of the utility model. DETAILED DESCRIPTION

[0022] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0023] See also Figure 1-4 A device for preparing a culture medium of Hirsutella serrata in China includes a frame 10, a preparation mechanism 20, a cleaning mechanism 30, a packaging mechanism 40, a conveying mechanism 50 and a PLC controller 60.

[0024] A ceiling 12 is provided on the top of the frame 10 , a control panel 13 electrically connected to the PLC controller 60 is embedded on the table top of the frame 10 , a double-door cabinet 14 is provided below the frame 10 , and universal wheels 15 are provided at the bottom of the frame 10 .

[0025] The preparation mechanism 20 and the cleaning mechanism 30 are arranged in the cabinet of the frame 10. One side of the table top of the frame 10 is a storage table 11, and the other side is provided with a filling mechanism 40 and a conveying mechanism 50; the storage table 11 is mainly used for transit and temporary storage. The culture bottles are quantitatively loaded into the cultivation bottles through the filling mechanism 40, and then the cultivation bottles are conveyed to the storage table 11 through the conveying device. After being covered, they are placed in a 121°C high-temperature and high-pressure steam sterilizer for sterilization for 30 minutes to obtain a culture medium suitable for the growth of Chinese Hirsutospore.

[0026] The preparation mechanism 20 is connected with the filling mechanism 40 and conveys the prepared culture medium liquid to the filling mechanism 40; specifically, the preparation mechanism 20 includes a preparation kettle 21 for storing the culture medium liquid, a power agitator 22 is arranged on the top of the inner cavity of the preparation kettle 21, the power agitator 22 is electrically connected to the PLC controller 60, and the start and stop and the speed can be controlled by the PLC controller 60; a temperature control device 23 is arranged on the bottom of the preparation kettle 21, the temperature control device 23 mainly includes a thermistor electrically connected to the PLC controller 60, and a temperature sensor. When working, the temperature sensor can monitor the temperature in the preparation kettle 21 and transmit the detection signal to the PLC controller 60 in real time. When the temperature in the preparation kettle 21 is lower than the set temperature, the thermistor is turned on for heating. When the temperature in the preparation kettle 21 reaches the set temperature, the thermistor is disconnected and the heating is stopped, thereby realizing automatic heating and boiling.

[0027] A pH reagent kit 25 is provided on the top of the preparation kettle 21. The pH reagent kit 25 is provided above the preparation kettle 21 and is divided into two containing compartments, respectively containing an acidic neutralizing liquid and an alkaline neutralizing liquid. The two containing compartments are connected to the preparation kettle 21 through a delivery pipe, and the delivery pipe is provided with an electromagnetic valve that can be controlled by a PLC controller 60. A pH sensor 26 is provided in the inner cavity of the preparation kettle 21. The pH sensor 26 is used to detect the acidity and alkalinity in the preparation kettle 21. When the pH value of the culture medium liquid in the preparation kettle 21 deviates from the set value, the electromagnetic valve of the delivery pipe of the pH reagent kit 25 containing compartment corresponding to the acidity or alkalinity is opened, and the pH value of the culture medium liquid is adjusted with the cooperation of the power agitator 22.

[0028] The cleaning mechanism 30 includes a water tank 31, a pressure pump 32, a water pipe 33 and a pressurized water gun 34. The input end of the pressure pump 32 is connected to the water tank 31 for extracting cleaning water, and the output end of the pressure pump 32 is connected to the pressure water gun 34 through the water pipe 33 for delivering high-pressure cleaning water. The pressure water gun 34 is set above the table of the frame 10, and a spring telescopic hose is set at the tail end. The spring telescopic hose extends from the top of the frame 10, and the length of the water pipe is adjusted according to the water demand; the pressure water gun 34 is suspended on the top of the frame 10 and is provided with a variety of water outlet modes, such as pressurized flushing, flushing, ordinary running water, etc.; it is used to clean or add water to the preparation kettle 21.

[0029] A water injection pipe 35 connected to an external water source is arranged on the top of the water tank 31, and a water injection valve 36 is arranged on the water injection pipe 35. A water level sensor 37 is arranged at a preset maximum liquid level on the inner wall of the water tank 31, and the water injection valve 36 and the water level sensor 37 are electrically connected to the PLC controller 60 respectively; the water level sensor 37 can monitor the water level in the water tank 31. When the water level is lower than the set height, the detection signal is fed back to the PLC controller 60, and the water injection valve 36 is controlled to open and inject water. When the water level reaches the set height, the detection signal is fed back to the PLC controller 60, and the water injection valve 36 is controlled to close and stop injecting water.

[0030] The dispensing mechanism 40 includes a dispensing machine body 41, and a dispensing pipe 42 is arranged at the front end of the dispensing machine body 41. The dispensing machine body 41 for liquid products is an existing device. This embodiment only refers to it and is not an improvement. The specific internal structure and working principle thereof are not described in detail in this embodiment. The dispensing pipe 42 is cantilevered and is located above the conveying mechanism 50. A conveying hose 24 is arranged in the preparation kettle 21, and the conveying hose 24 is connected to the dispensing mechanism 40. The dispensing machine body 41 draws culture medium liquid through the conveying hose 24, and puts the culture medium liquid into the cultivation bottle through the dispensing pipe 42. The cultivation bottle is transferred to the storage table 11 through the conveying mechanism 50.

[0031] The conveying mechanism 50 is arranged below the filling mechanism 40 and is used for intermittently delivering the cultivation bottles to be filled in a flowing manner. The conveying mechanism 50 adopts a conveying device driven by a rubber transmission belt and a roller. The roller is driven by a motor, and the motor is controlled by a PLC controller 60. The filling machine body 41 and the conveying mechanism 50 are electrically connected to the PLC controller 60 respectively. The filling time of the filling machine body 41 and the conveying speed of the conveying mechanism 50 can be controlled by parameter setting.

[0032] Working principle:

[0033] When in use, the culture medium ingredients are added to the preparation kettle 21 in proportion, the mixed liquid is heated by the temperature control device 23, the mixed liquid is evenly stirred by the power stirrer 22, the parameters are set by the PLC controller 60, and the filling machine body 41 is controlled to quantitatively draw the culture medium liquid through the conveying hose 24, and then transfer it to the cultivation bottle on the conveying mechanism 50 through the filling tube 42, and then transfer it to the storage table 11 through the conveying mechanism 50. The cultivation bottle is then sterilized with high temperature and high pressure steam at 121°C for 30 minutes to finally obtain a culture medium suitable for Chinese Hirsutella serrata.

[0034] Any equivalent structure or equivalent process transformation made using the contents of the specification and drawings of this utility model, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of this utility model.

Claims

1. A device for preparing a culture medium of Hirsutella sinensis, characterized in that: It comprises a frame (10), wherein a preparation mechanism (20) and a cleaning mechanism (30) are arranged in a cabinet of the frame (10), one side of the table top of the frame (10) is a storage table (11), and the other side is provided with a dispensing mechanism (40) and a conveying mechanism (50); The preparation mechanism (20) is connected to the packaging mechanism (40) and delivers the prepared culture medium liquid to the packaging mechanism (40); The conveying mechanism (50) is arranged below the dispensing mechanism (40) and is used for intermittently conveying the cultivation bottles to be filled in a flowing manner; The cleaning mechanism (30) comprises a water storage tank (31), a pressure pump (32), a water pipe (33) and a pressure water gun (34); the input end of the pressure pump (32) is connected to the water storage tank (31) for extracting cleaning water; the output end of the pressure pump (32) is connected to the pressure water gun (34) through the water pipe (33) for conveying high-pressure cleaning water; the pressure water gun (34) is mounted above the table of the frame (10) and a spring-loaded telescopic hose is arranged at the tail end; It also includes a PLC controller (60) for controlling the preparation mechanism (20), the cleaning mechanism (30), the packaging mechanism (40) and the conveying mechanism (50).

2. A device for preparing a culture medium of Hirsutella sinensis as claimed in claim 1, characterized in that: The top of the water storage tank (31) is provided with a water injection pipe (35) connected to an external water source, the water injection pipe (35) is provided with a water injection valve (36), and a water level sensor (37) is provided at a preset maximum liquid level on the inner wall of the water storage tank (31), and the water injection valve (36) and the water level sensor (37) are respectively electrically connected to a PLC controller (60).

3. A device for preparing a culture medium of Hirsutella sinensis as claimed in claim 1, characterized in that: The preparation mechanism (20) comprises a preparation kettle (21) for storing culture medium liquid, a power stirrer (22) is arranged at the top of the inner cavity of the preparation kettle (21), a temperature control device (23) is arranged at the bottom of the preparation kettle (21), a conveying hose (24) is arranged in the preparation kettle (21), and the conveying hose (24) is connected to the packaging mechanism (40).

4. A device for preparing a culture medium of Hirsutella sinensis as claimed in claim 3, characterized in that: A pH reagent kit (25) is arranged on the top of the preparation kettle (21), and a pH sensor (26) is arranged in the inner cavity of the preparation kettle (21).

5. A device for preparing a culture medium of Hirsutella sinensis as claimed in claim 1, characterized in that: The dispensing mechanism (40) comprises a dispensing machine body (41), a dispensing pipe (42) being arranged at the front end of the dispensing machine body (41), the dispensing pipe (42) being arranged in a cantilevered manner and being located above the conveying mechanism (50).

6. A device for preparing a culture medium of Hirsutella sinensis as claimed in claim 1, characterized in that: A ceiling (12) is arranged on the top of the frame (10); a control panel (13) electrically connected to a PLC controller (60) is embedded on the table top of the frame (10); a double-door cabinet (14) is arranged below the frame (10); and universal wheels (15) are arranged at the bottom of the frame (10).

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