Device for automatically synthesizing nanoparticles

Abstract

The utility model provides a device for automatically synthesizing nanoparticles, comprising: a pressure supply module, which is provided with more than one air outlet corresponding to an inlet of a micro-fluidic chip so as to supply air to the inlet of the micro-fluidic chip or stop supplying air to the inlet of the micro-fluidic chip through the air outlet; the liquid transferring module can transfer liquid to the inlet of the micro-fluidic chip; the movement module can drive the pressure supply module and the pipetting module to move in the horizontal direction and the vertical direction. According to the device provided by the invention, the nano-particles can be automatically synthesized by the micro-fluidic chips, and particularly, when a plurality of reaction combinations are arranged on each micro-fluidic chip and / or a plurality of micro-fluidic chips are arranged on the bottom plate of the device, the same or different liquid nano-particles can be prepared more efficiently.

Description

Technical Field

[0001] The present application relates to the technical field of analysis systems, and in particular to a device for automatically synthesizing nanoparticles. Background Art

[0002] Nanoparticle synthesis technology is at the forefront of the rapidly developing field of nanotechnology. Its unique size-dependent characteristics make these materials have great advantages in many fields and are in an irreplaceable position. This technology has been widely used in many industries, such as drug delivery, energy, and electronics. Nanoparticle synthesis technology is one of the key steps to achieve the application of nanoparticles. Microreactors based on microfluidic technology can achieve rapid mixing of reagents, temperature control, and precise spatiotemporal manipulation of reactions. Using microfluidic technology for nanoparticle synthesis, the mixing is controlled and uniform, and nanoparticles of uniform size can be produced. At the same time, the repeatability of the physical and chemical properties of nanoparticles can also be precisely controlled. In addition, by regulating the microenvironment of nanoparticle synthesis, the size uniformity and repeatability of nanoparticles can be further improved, thereby improving the yield of the nanoparticle preparation process.

[0003] The formulation of nanoparticles plays a very important role in drug encapsulation efficiency, particle size, particle size uniformity, surface charge, transfection efficiency, etc. Therefore, when developing nanoparticle drugs, it is necessary to screen a large number of nanoparticle formula libraries based on drug types and transfection targets; in addition to the formula of nanoparticles, the type of drug and drug modification will also have a great impact on the actual effect of the drug (such as the protein expression level of mRNA drugs). Therefore, in addition to screening the formula of nanoparticles, it is also necessary to screen the drug library. In summary, in the process of drug development, it is often necessary to screen large-scale nanoparticle formula libraries and drug libraries.

[0004] Existing nanoparticle synthesis equipment based on microfluidic technology is an instrument that synthesizes a single or small batch of samples in a single operation. When conducting large-scale screening of nanoparticle formulations and drug formulations, it requires a lot of manpower and time. Utility Model Content

[0005] In order to solve the problems in the prior art, the present application provides a device for automatically synthesizing nanoparticles. The technical solution of the present application is as follows:

[0006] A device for automatically synthesizing nanoparticles, comprising:

[0007] A pressure supply module, wherein the pressure supply module is provided with one or more gas outlets corresponding to the inlet of the microfluidic chip, so as to be able to supply gas to the inlet of the microfluidic chip or stop supplying gas through the gas outlets;

[0008] A pipetting module, which is capable of pipetting liquid to an inlet of the microfluidic chip;

[0009] A motion module, wherein the motion module can drive the pressure supply module and the liquid transfer module to move in a horizontal direction and in a vertical direction.

[0010] Furthermore, the pressure supply module comprises: a pressure plate assembly; and an air supply assembly, wherein the air supply assembly can supply air to the air outlet.

[0011] Furthermore, the pressure plate assembly includes: a pressure plate body; an air inlet and the air outlet, both of which are arranged on the pressure plate body; and a gas flow channel, which is arranged in the pressure plate body to connect the air inlet and the air outlet.

[0012] Further, the air inlet includes at least a first air inlet and a second air inlet; the air outlet includes at least a first air outlet and a second air outlet; the gas flow channel includes at least a first flow channel and a second flow channel; wherein, the first air outlet is used to supply or stop gas supply to the first phase inlet of the microfluidic chip; the second air outlet is used to supply or stop gas supply to the second phase inlet of the microfluidic chip; the first flow channel is used to connect the first air inlet and the first air outlet; the second flow channel is used to connect the second air inlet and the second air outlet.

[0013] Further, the pressure plate assembly also includes: a first control valve and a second control valve, each of which includes at least a first interface, a second interface, and a third interface, and can switch between connecting the first interface with the second interface and connecting the first interface with the third interface; a first connection port group and a second connection port group, both of which are arranged on the pressure plate body, and each of which includes at least a first connection port, a second connection port, and a third connection port; wherein the first interface of the first control valve can be connected to the first air outlet through the first connection port of the first connection port group and a gas flow channel, the second interface of the first control valve can be connected to the first air inlet through the second connection port of the first connection port group and a gas flow channel, and the third interface of the first control valve is connected to the atmosphere; the first interface of the second control valve can be connected to the second air outlet through the first connection port of the second connection port group and a gas flow channel, the second interface of the second control valve can be connected to the second air inlet through the second connection port of the second connection port group and a gas flow channel, and the third interface of the second control valve is connected to the atmosphere.

[0014] Furthermore, the gas supply assembly includes at least: a first gas tank and a second gas tank, wherein the inlet of the first gas tank and the inlet of the second gas tank are each independently connected to a gas source; wherein the outlet of the first gas tank is connected to the first gas inlet; and the outlet of the second gas tank is connected to the second gas inlet.

[0015] Furthermore, the pressure supply module further includes: a control component, which independently controls the gas source to supply gas to the first gas storage tank and the second gas storage tank.

[0016] Furthermore, the pressure supply module also includes: a sealing gasket, on which a through hole corresponding to the air outlet is arranged, and the sealing gasket is arranged on one side of the pressure plate body where the air outlet is arranged, so as to seal when the air outlet supplies air to the inlet of the microfluidic chip or stops supplying air.

[0017] Furthermore, a filter element is arranged on a through hole of at least one of the sealing gaskets corresponding to the air outlet.

[0018] Furthermore, the pressure supply module further comprises: a sealing gasket fixing assembly, which is capable of fixing the sealing gasket on a side of the pressure plate body where the air outlet is arranged and detaching the sealing gasket from the pressure plate body.

[0019] Furthermore, a magnetic channel is provided on the pressure plate body; the sealing gasket fixing assembly is located on the opposite side of the pressure plate body where the air outlet is provided; the sealing gasket fixing assembly includes: a magnetic unit; a first moving unit, the first moving unit can drive the magnetic unit to pass through the magnetic channel to magnetically attract the sealing gasket, and can drive the magnetic unit away from the sealing gasket to make the sealing gasket detach from the pressure plate body.

[0020] Furthermore, the first motion unit includes: a magnetic unit fixing plate, the magnetic unit fixing plate fixing the magnetic unit; and a first motor, the first motor being capable of driving the magnetic unit fixing plate to move closer to or away from the pressure plate body.

[0021] Furthermore, the pipetting module comprises: a pipetting unit, and the pipetting unit is capable of sucking in and discharging liquid.

[0022] Furthermore, the pipetting unit is a pipette, and the pipette is a single-channel pipette or a multi-channel pipette; the pipetting module also includes: a second motion unit, which can drive the control button of the pipette to perform pipetting; and a third motion unit, which can drive the tip removal button of the pipette to remove the gun tip.

[0023] Furthermore, the motion module includes: a fixed component; a horizontal motion component, which can drive the fixed component to move in the horizontal direction; a vertical motion component, on which the vertical motion component, the pressure supply module and the pipetting module are arranged, and the vertical motion component can independently drive the pressure supply module and the pipetting module to move in the vertical direction.

[0024] Furthermore, the horizontal motion component includes: a first horizontal motion unit, which can drive the fixed component to move in a first horizontal direction; and a second horizontal motion unit, which can drive the first horizontal motion unit to move in a second horizontal direction.

[0025] Further, the first horizontal motion unit includes: a first guide rail, the first guide rail is arranged along a first horizontal direction, and a fourth motion unit, the fourth motion unit can drive the fixed component to slide along the first guide rail; and / or, the second horizontal motion unit includes: a second guide rail, the second guide rail is arranged along a second horizontal direction, and a fifth motion unit, the fifth motion unit can drive the first horizontal motion unit to slide along the second guide rail.

[0026] Furthermore, the vertical motion assembly includes: a first vertical motion unit, which can drive the pressure supply module to move in the vertical direction; and a second vertical motion unit, which can drive the pipetting module to move in the vertical direction.

[0027] Furthermore, the device further comprises: a box module, the box module accommodating the pressure supply module, the pipetting module and the motion module; and an air filter module, the air filter module being arranged on the box module.

[0028] The device for automatically synthesizing nanoparticles provided by the present application can realize the automatic synthesis of nanoparticles on a microfluidic chip. When a plurality of the above reaction combinations are arranged on each microfluidic chip, and a corresponding number of gas outlets are arranged on the pressure supply module as the inlet of the microfluidic chip, multiple reactions can be carried out simultaneously to prepare the same or different liquid nanoparticles. In particular, when a plurality of such microfluidic chips are arranged on the bottom plate, the same or different liquid nanoparticles can be prepared more efficiently.

[0029] The above description is only an overview of the technical solution of the present application. In order to make the technical means of the present application clearer and to enable those skilled in the art to implement it according to the contents of the specification, and to make the above and other purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are exemplified below. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 : A schematic diagram of a device for automatically synthesizing nanoparticles in one embodiment;

[0031] Figure 2 : A schematic diagram of the relative positions of a fixed component, a pressure supply module, a liquid transfer module, and a control component in one embodiment;

[0032] Figure 3 : A schematic diagram of the relative positions of a fixed component, a pressure supply module, and a liquid transfer module in one embodiment;

[0033] Figure 4 : A schematic diagram of the structure of a microfluidic chip in one embodiment;

[0034] Figure 5 : A schematic diagram of the relative positions of the pressure plate assembly and the microfluidic chip during liquid nanoparticle synthesis in one embodiment;

[0035] Figure 6 : A schematic diagram of the structure of a pressure plate assembly in one embodiment, in which the magnetic suction channel is not shown;

[0036] Figure 7 : A schematic diagram of the relative positions of the pressing plate body and the air supply assembly in one embodiment;

[0037] Figure 8 : A schematic diagram of the structure of a sealing gasket fixing assembly and a pressure plate assembly in one embodiment;

[0038] Fig. 9 : Schematic diagram of the sealing gasket structure in one embodiment.

[0039] Description of reference numerals:

[0040] 1000, pressure supply module;

[0041] 1100, pressure plate assembly;

[0042] 1110, platen body; 1120, air inlet; 1121, first air inlet; 1122, second air inlet; 1130, air outlet; 1131, first air outlet; 1132, second air outlet; 1150, first control valve; 1160, second control valve; 1171, first connection port group; 1172, second connection port group; 1180, air outlet channel; 1190, magnetic suction channel;

[0043] 1200, sealing gasket; 1210, iron sheet;

[0044] 1300, sealing pad fixing assembly; 1310, magnetic unit; 1321, magnetic unit fixing plate; 1322, first motor; 1323, screw;

[0045] 1410, first gas storage tank; 1411, entrance of the first gas storage tank; 1420, second gas storage tank; 1421, entrance of the second gas storage tank;

[0046] 1500, control component; 1600, pressure pump;

[0047] 2000, pipetting module;

[0048] 3100, fixing assembly; 3211, first guide rail; 3221, second guide rail;

[0049] 4000, cabinet module; 4100, base plate.

[0050] 5000, microfluidic chip; 5100, microfluidic chip inlet; 5110, first phase inlet; 5120, second phase inlet; 5200, microfluidic chip outlet;

[0051] 6000. Chip tray. DETAILED DESCRIPTION

[0052] The following embodiments of the present application are only used to illustrate the specific embodiments of the present application, and these embodiments cannot be understood as limiting the present application. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principle of the present application are deemed to be equivalent replacement methods and fall within the scope of protection of the present application.

[0053] Those skilled in the art should understand that, in the disclosure of the present application, the terms "first", "second", "third", "fourth", "fifth", etc. are only used to distinguish different structures, but do not limit the number, connection relationship, etc. of specific structures; in addition, the directions or positional relationships indicated by "longitudinal", "transverse", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc. are based on the directions or positional relationships shown in the accompanying drawings, which are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation. Therefore, the above terms cannot be understood as limitations on the present application.

[0054] This embodiment provides a device for automatically synthesizing nanoparticles, such as Figures 1 to 9 As shown, it includes:

[0055] A pressure supply module 1000, wherein the pressure supply module 1000 is provided with one or more gas outlets 1130 corresponding to the inlet of the microfluidic chip 5000 (microfluidic chip inlet 5100), so as to be able to supply gas to the inlet of the microfluidic chip or stop supplying gas through the gas outlets 1130;

[0056] A pipetting module 2000, wherein the pipetting module 2000 is capable of transferring liquid to the inlet of the microfluidic chip 5000 (the microfluidic chip inlet 5100); preferably, the pipetting module 2000 is also capable of transferring liquid from the outlet of the microfluidic chip 5000 (the microfluidic chip outlet 5200);

[0057] The motion module can drive the pressure supply module 1000 and the pipetting module 2000 to move in a horizontal direction and a vertical direction.

[0058] Regarding the microfluidic chip 5000 for synthesizing nanoparticles, Figure 4 As shown, generally, it includes a microfluidic chip inlet 5100 and a microfluidic chip outlet 5200, and the microfluidic chip inlet 5100 and the microfluidic chip outlet 5200 are generally configured as a liquid storage tank (the volume in this embodiment is 1μl~10ml). Among them, the microfluidic chip inlet 5100 is further divided into a first phase inlet 5110 and a second phase inlet 5120 (the first phase inlet 5110 and the second phase inlet 5120 can independently store aqueous phase liquid and organic phase liquid as needed, and in this example, they are used to store aqueous phase liquid and organic phase liquid respectively), a first phase inlet 5110, a second phase inlet 5120, a microfluidic chip outlet 5200 and the flow channels connecting them constitute a reaction combination (a plurality of such reaction combinations can be set on the same microfluidic chip 5000, such as Figure 4 As shown, a microfluidic chip 5000 including four reaction combinations is exemplarily provided. When the microfluidic chip 5000 is used, the first phase inlet 5110 and the second phase inlet 5120 can independently store aqueous phase liquid and organic phase liquid (which can be liquids of the same phase or liquids of different phases) as needed, and then the first phase inlet 5110 and the second phase inlet 5120 are pressurized, and the stored liquids are mixed in the flow channel to form nanoparticles (for example, drugs) that enter the microfluidic chip outlet 5200 and are stored for subsequent analysis to perform quality control on drug encapsulation effects, etc.

[0059] Those skilled in the art know that the spatial motion of an object includes six degrees of freedom, including translational motion in three directions and rotational motion in three directions. In the present application, the motion in the horizontal direction and the motion in the vertical direction refer to at least a translational motion in one direction (such as the x direction or the y direction) on the horizontal direction (such as the horizontal plane direction formed by the x direction and the y direction) and a translational motion in the vertical direction (such as the z direction perpendicular to the horizontal plane direction formed by the x direction and the y direction), preferably, the translational motion can be performed in three directions (such as the above-mentioned x direction, y direction, and z direction). In addition, those skilled in the art know that the motion in the horizontal direction and the motion in the vertical direction can be performed successively in one direction to achieve motion in multiple directions (such as two directions or three directions), or can be performed simultaneously in multiple directions (such as two directions or three directions). Regarding the motion in the vertical direction, the present application preferably provides that the motion module can independently drive the pressure supply module 1000 and the pipetting module 2000 to move in the vertical direction.

[0060] The device for automatically synthesizing nanoparticles provided in the present application is used to realize the automatic synthesis of nanoparticles by the above-mentioned microfluidic chip 5000. When the above-mentioned microfluidic chip 5000, a container storing liquid phase liquid (such as aqueous phase and organic phase) (such as a storage well plate, a liquid reservoir, a centrifuge tube, etc.), and a collection container (such as a collection well plate, a centrifuge tube, etc.) are placed on the bottom plate, first, the motion module drives the pipetting module 2000 to move to the upper part of the container storing liquid phase liquid (such as aqueous phase and organic phase), and the pipetting module 2000 absorbs liquid; then, the motion module drives the pipetting module 2000 to move to the upper part of the inlet of the microfluidic chip 5000 (such as pipetting to the first phase inlet 5110 and the second phase inlet 5120) to add liquid to the inlet of the microfluidic chip 5000 (such as pipetting to the first phase inlet 5110 and the second phase inlet 5120); then, the motion module drives the pressure supply module 1000 to move to the microfluidic chip 5000. The motion module drives the pipetting module 2000 to move to the upper part of the outlet (microfluidic chip outlet 5200) of the microfluidic chip 5000, and the pipetting module 2000 aspirates liquid (absorbs liquid nanoparticles generated by the reaction); finally, the motion module drives the pipetting module 2000 to move to the upper part of the collection container, and the pipetting module 2000 stores the aspirated liquid nanoparticles in the collection container for subsequent use (such as purification, cell transfection, etc.).

[0061] When each microfluidic chip 5000 is provided with a plurality of the above-mentioned reaction combinations (including a first phase inlet 5110, a second phase inlet 5120, a microfluidic chip outlet 5200 and a flow channel connecting them), the pressure supply module 1000 is provided with a corresponding number of gas outlets 1130 as the inlet of the microfluidic chip 5000, so that multiple reactions can be carried out simultaneously to prepare the same or different liquid nanoparticles. In particular, when a plurality of such microfluidic chips 5000 are provided on the bottom plate, the same or different liquid nanoparticles can be prepared more efficiently. When a plurality of microfluidic chips 5000 are provided, the plurality of microfluidic chips 5000 can be placed on a chip tray 6000 to facilitate the overall placement of the plurality of microfluidic chips 5000 into or removal from the device.

[0062] In addition, the device of the present application can further include a box module 4000, which accommodates the pressure supply module 1000, the pipetting module 2000 and the motion module, so that the entire device can be realized as a closed environment to ensure the cleanliness of the inside of the device. In addition, an air filter module (such as a filter / filter element, not shown in the figure) can also be set on the box module 4000 to further ensure the cleanliness of the inside of the device.

[0063] The device of the present application can also include a temperature control module to perform temperature control on reagents in containers (such as orifice plates, liquid reservoirs, etc.) storing liquid phase liquids (such as aqueous phases and organic phases), and / or to perform temperature control on the liquid nanoparticle synthesis process of the microfluidic chip 5000 to perform heating or cooling or heat preservation. Specifically, the temperature control module may include a heating component and / or a cooling component, wherein the heating component may be an electric heating component and the cooling component may be an air cooling component. The temperature control module may be specifically arranged on the bottom plate of the device and / or on the chip tray 6000.

[0064] In one embodiment, Figures 1 to 9 As shown, especially Figure 6 , Figure 7 As shown, the pressure supply module 1000 includes: a pressure plate assembly 1100 , and an air supply assembly 1400 . The air supply assembly 1400 can supply air to the air outlet 1130 .

[0065] Specifically, the pressing plate assembly 1100 includes:

[0066] Pressing plate body 1110;

[0067] The air inlet 1120 and the air outlet 1130 are both arranged on the pressing plate body 1110;

[0068] A gas flow channel is disposed in the pressing plate body 1110 to connect the gas inlet 1120 and the gas outlet 1130 .

[0069] Thus, gas outlets 1130 corresponding to the number and positions of the inlets (first phase inlet 5110 and second phase inlet 5120) of the microfluidic chip 5000 can be set on the pressure plate body 1110 to supply gas to the inlet of the microfluidic chip 5000, and the pressure plate body 1110 is also provided with an air inlet 1120 and a gas flow channel for connecting the air inlet 1120 and the air outlet 1130, so that gas can be supplied from the air inlet 1120 to the air outlet 1130, thereby achieving the goal of pressing the upper part of the microfluidic chip 5000 through the pressure plate body 1110, so that the air outlet 1130 corresponds to and is connected with the inlet of the microfluidic chip 5000, so as to supply gas to the inlet of the microfluidic chip 5000, and prepare liquid nanoparticles through the microfluidic chip 5000.

[0070] In addition, if Figure 6 As shown, the pressure plate assembly 1100 may further include an air outlet channel 1180, which is arranged on the pressure plate body 1110 and corresponds to the microfluidic chip outlet 5200 of the microfluidic chip, so that when the pressure plate assembly 1100 presses down the upper part of the microfluidic chip, the microfluidic chip outlet 5200 is connected to the atmosphere, so as to facilitate the microfluidic chip 5000 to synthesize liquid nanoparticles.

[0071] Furthermore, if Figure 6 As shown, the air inlet 1120 includes at least a first air inlet 1121 and a second air inlet 1122. In this embodiment, the first air inlet 1121 and the second air inlet 1122 are both provided as one;

[0072] The air outlet 1130 at least includes a first air outlet 1131 and a second air outlet 1132. In this embodiment, the first air outlet 1131 and the second air outlet 1132 are both provided in plurality, specifically four.

[0073] The gas flow channel at least includes a first flow channel and a second flow channel;

[0074] in,

[0075] The first gas outlet 1131 is used to supply gas to the first phase inlet 5110 of the microfluidic chip 5000 or stop supplying gas;

[0076] The second gas outlet 1132 is used to supply gas to or stop supplying gas to the second phase inlet 5120 of the microfluidic chip 5000;

[0077] The first flow channel is used to connect the first air inlet 1121 and the first air outlet 1131;

[0078] The second flow channel is used to connect the second air inlet 1122 and the second air outlet 1132 .

[0079] Right now, Figure 6In the illustrated pressing plate assembly 1100, the first gas inlet 1121 is connected to the first gas outlet 1131 through a gas flow channel (first flow channel); the second gas inlet 1122 is connected to the second gas outlet 1132 through a gas flow channel (second flow channel). Thus, gas can be delivered to the first gas outlet 1131 through the first gas inlet 1121, and gas can be delivered to the second gas outlet 1132 through the second gas inlet 1122; so as to realize independent gas supply to the first phase inlet 5110 and the second phase inlet 5120 of the microfluidic chip 5000, so that gas can be supplied to different inlets (first phase inlet 5110 and second phase inlet 5120) with different pressures, so as to respectively control the flow rate ratio of different phase liquids and the regulation of the total flow rate, so as to realize the regulation of the nanoparticle synthesis process and the physical properties of the nanoparticles (including size, surface charge, particle size uniformity, etc.).

[0080] Figure 6 The pressure plate assembly 1100 shown adopts a non-pipeline connection mode. When the pressure plate assembly 1100 presses down the microfluidic chip 5000, the gas path that needs to be inflated is limited to the gas path in the pressure plate structure, thereby minimizing the dead volume of the gas path, increasing the speed of pressurization when the control valve (first control valve 1150; second control valve 1160) is switched to allow the air inlet 1120 (first air inlet 1121, second air inlet 1122) to supply air to the inlet (first phase inlet 5110, second phase inlet 5120) of the microfluidic chip 5000, so that the pressure in the inlet of the microfluidic chip 5000 reaches the set pressure more quickly, that is, the driven liquid can quickly reach the set flow rate, and increase the uniformity of the synthesized nanoparticles (the lower flow rate in the initial stage will result in the production of nanoparticles with larger particle sizes).

[0081] In one embodiment, Figure 6 , Figure 7 As shown, the pressing plate assembly 1100 further includes:

[0082] The first control valve 1150 and the second control valve 1160 each include at least a first interface, a second interface, and a third interface, and can switch between the first interface being connected to the second interface and the first interface being connected to the third interface;

[0083] The first connection port group 1171 and the second connection port group 1172 are both arranged on the pressing plate body 1110, and each includes at least a first connection port, a second connection port, and a third connection port (such as Figure 6 As shown, in the first connection port group 1171 and the second connection port group 1172, from top to bottom are the second connection port, the first connection port, and the third connection port);

[0084] in,

[0085] The first interface of the first control valve 1150 can be connected to the first gas outlet 1131 through the first connection port of the first connection port group 1171 and the gas flow channel, the second interface of the first control valve 1150 can be connected to the first gas inlet 1121 through the second connection port of the first connection port group and the gas flow channel, and the third interface of the first control valve 1150 is in communication with the atmosphere;

[0086] The first interface of the second control valve 1160 can be connected to the second air outlet 1132 through the first connection port of the second connection port group 1172 and the gas flow channel, the second interface of the second control valve 1160 can be connected to the second air inlet through the second connection port of the second connection port group 1172 and the gas flow channel, and the third interface of the second control valve 1160 is connected to the atmosphere.

[0087] Figure 6 , Figure 7 The pressure plate assembly 1100 shown is further provided with a first control valve 1150 and a second control valve 1160 .

[0088] For the first control valve 1150, when the first interface is connected with the second interface, the first air inlet 1121 is connected with the first air outlet 1131, so that gas can be supplied to the first phase inlet 5110 through the first air inlet 1121, the gas flow channel, and the first air outlet 1131; when the first interface is connected with the third interface, the first air outlet 1131 is connected with the atmosphere, thereby stopping the gas supply to the first phase inlet 5110, and the first phase inlet 5110 is connected with the atmosphere.

[0089] For the second control valve 1160, when the first interface is connected with the second interface, the second air inlet 1122 is connected with the second air outlet 1132, so that gas can be supplied to the second phase inlet 5120 through the second air inlet 1122, the gas flow channel, and the second air outlet 1132; when the first interface is connected with the third interface, the gas supply to the second phase inlet 5120 is stopped, and the second phase inlet 5120 is connected to the atmosphere.

[0090] That is, by providing the first control valve 1150 and the second control valve 1160 , it is possible to independently supply gas to the first phase inlet 5110 and the second phase inlet 5120 or stop supplying gas.

[0091] The control of the first control valve 1150 and the second control valve 1160 may be implemented through a PLC or a single chip microcomputer, for example, they may be controlled together through a control component 1500 provided below.

[0092] In one embodiment, Figure 7 As shown,

[0093] The gas supply assembly 1400 includes at least:

[0094] A first gas storage tank 1410 and a second gas storage tank 1420, wherein an inlet 1411 of the first gas storage tank and an inlet 1421 of the second gas storage tank are independently connected to a gas source;

[0095] in,

[0096] The outlet of the first gas storage tank is connected to the first gas inlet 1121;

[0097] The outlet of the second gas storage tank is connected to the second gas inlet 1122 .

[0098] Regarding the gas source, the present application has no specific restrictions, as long as it can achieve stable gas supply, and specific examples include gas tanks, pressure pumps, etc. In this embodiment, a pressure pump 1600 (air pump) is used.

[0099] In this embodiment, the gas is not directly supplied to the gas inlet 1120 (the first gas inlet 1121 and the second gas inlet 1122) through the gas source, but the first gas storage tank 1410 and the second gas storage tank 1420 are arranged between the gas source and the gas inlet 1120 (the first gas inlet 1121 and the second gas inlet 1122). When the gas source pressure is unstable, or when the control valve (the first control valve 1150 and the second control valve 1160) controls the gas supply to the inlet of the microfluidic chip, the gas storage tank can buffer the gas provided by the gas source, and can ensure that the pressure stability of the entire gas path is maintained when pressurizing the inlet of the microfluidic chip, so as to ensure that the liquid nanoparticles can be stably synthesized.

[0100] In one embodiment, Figure 1 , Figure 2 As shown in the

[0101] The pressure supply module 1000 further includes:

[0102] The control component 1500 independently controls the gas source to supply gas to the first gas tank 1410 and the second gas tank 1420, for example, it can control whether to supply gas and control the pressure of the supplied gas, that is, the control component independently controls the gas source to supply gas to the first gas tank and the second gas tank, including: the control component independently controls whether the gas source supplies gas to the first gas tank and the second gas tank and the pressure of the supplied gas.

[0103] Specifically, the control component 1500 can be in the form of a PLC or a single chip microcomputer. When the gas source is a pressure pump, the pressure of the gas supply can be controlled by electrically connecting the control component 1500 to the pressure pump; when the gas source is a constant pressure gas source, a proportional valve can be set on the gas source gas supply pipeline, and a pressure sensor can be set on the gas source gas supply pipeline or in the first gas storage tank 1410 and the second gas storage tank 1420. The output gas pressure is controlled by electrically connecting the control component 1500 to the proportional valve and the pressure sensor, thereby realizing the control of the gas source output pressure.

[0104] In addition, the control component 1500 can also be electrically connected to the control valves described in this specification (such as the first control valve 1150, the second control valve 1160, etc.); the motors in the lead screws / electric push rods involved in the first motion unit to the fifth motion unit, the first vertical motion unit, the second vertical motion unit, etc., and the control valves of the cylinders involved; and the heating components and cooling components involved in the temperature control module, so as to further realize the automatic control of the device of this application.

[0105] In one embodiment, Figure 5 , Figure 8 , Fig. 9 As shown in FIG. 1 , the pressure supply module 1000 further includes:

[0106] The sealing gasket 1200 is provided with through holes corresponding to the air outlet 1130 and the air outlet channel 1180. The sealing gasket 1200 is arranged on a side of the pressure plate body 1110 where the air outlet is arranged (in this embodiment, the lower side of the pressure plate body 1110) to seal when the air outlet 1130 supplies air to the inlet of the microfluidic chip 5000 or stops supplying air.

[0107] Preferably, a filter is provided on at least one (such as one, some or all, preferably all) of the through holes of the sealing gasket. The filter installed on the sealing gasket can prevent the liquid in the microfluidic chip inlet 5100 from splashing into the gas path, and at the same time, it can also prevent dust and particles in the gas providing pressure from entering the liquid storage tank, thereby ensuring that the microfluidic chip 5000 can stably synthesize liquid nanoparticles.

[0108] As for the material of the sealing gasket, commonly used rubber can be selected.

[0109] In one embodiment, Figure 5 , Figure 8 , Fig. 9 As shown, the pressure supply module 1000 further includes:

[0110] The sealing gasket fixing assembly 1300 can fix the sealing gasket 1200 on the side of the pressure plate body 1110 where the air outlet is set (in this embodiment, the lower side of the pressure plate body 1110) and detach the sealing gasket 1200 from the pressure plate body 1110.

[0111] The present application has no specific limitation on the manner in which the sealing gasket fixing assembly 1300 fixes the sealing gasket 1200, and specific methods include magnetic attraction, clamping claws, etc.

[0112] That is, the device provided in this embodiment is provided with the sealing gasket fixing assembly 1300 , so that the sealing gasket 1200 can be fixed and replaced by the sealing gasket fixing assembly 1300 .

[0113] In one embodiment, Figure 8 , 9 As shown, a magnetic attraction channel 1190 is provided on the pressing plate body 1110;

[0114] The sealing gasket fixing assembly is located on the opposite side of the pressing plate body 1110 where the air outlet is provided (in this embodiment, the upper side of the pressing plate body 1110);

[0115] The sealing gasket fixing assembly 1300 includes:

[0116] Magnetic unit 1310;

[0117] The first moving unit can drive the magnetic unit to pass through the magnetic channel 1190 to magnetically attract the sealing gasket 1200 , and can drive the magnetic unit 1310 away from the sealing gasket 1200 to make the sealing gasket detach from the pressure plate body 1110 .

[0118] That is, in this embodiment, a solution is provided in which the sealing gasket fixing assembly 1300 fixes the sealing gasket 1200 by magnetic attraction.

[0119] Regarding magnetic attraction, the magnetic attraction unit 1310 and the magnetically attracted parts of the sealing gasket 1200 can be a combination of magnets and iron materials, or can be set as magnets that attract each other. Specifically, in this embodiment, the magnetic attraction unit 1310 is a magnetic bar, and an iron sheet 1210 corresponding to the magnetic attraction unit 1310 is set on the sealing gasket 1200.

[0120] There is no specific restriction in this application regarding the first motion unit. In this embodiment, the magnetic unit 1310 is driven to move up and down by a lead screw. Specifically, the first motion unit includes: a magnetic unit fixing plate 1321, which fixes the magnetic unit 1310; a first motor 1322, which can drive the magnetic unit fixing plate to move closer to or away from the pressure plate body 1110 through a screw 1323. Of course, those skilled in the art will know that the first motion unit may also include a cylinder and a magnetic unit fixing plate 1321, and the cylinder drives the magnetic unit 1310 to move up and down through the magnetic unit fixing plate 1321.

[0121] When using the device of the present application, multiple sealing pads 1200 can be further placed on the bottom plate, and a recovery area can be set. When the motion module drives the pressure supply module 1000 to move to the upper part of the sealing pad 1200, when the first motion unit drives the magnetic unit 1310 to move downward, the magnetic unit 1310 can magnetically attract the iron sheet on the sealing pad 1200, so that the sealing pad 1200 is fixed at the bottom of the pressure plate body 1110; when a reaction for preparing liquid nanoparticles is completed, the motion module drives the pressure supply module 1000 to move to the recovery area, the first motion unit drives the magnetic unit 1310 to move upward, and the magnetic unit 1310 is away from the sealing pad 1200, so that the sealing pad 1200 is detached from the pressure plate body 1110, thereby achieving the purpose of replacing the sealing pad 1200, so that the sealing pad 1200 can be automatically replaced after a single preparation synthesis.

[0122] In one embodiment, Figure 2 , Figure 3 As shown, the pipetting module 2000 includes: a pipetting unit, which can inhale and discharge liquid. Thus, the pipetting unit can transfer liquid from a container storing liquid phase liquid (such as aqueous phase and organic phase) to the inlet (such as the first phase inlet 5110 and the second phase inlet 5120) of the microfluidic chip 5000, and transfer liquid from the outlet (such as the microfluidic chip outlet 5200) of the microfluidic chip 5000 to a collection container (such as a well plate / centrifuge tube).

[0123] Specifically, in this embodiment, the pipetting unit is a pipette. The pipette may be a single-channel pipette or a multi-channel pipette. In order to add liquid to all inlets of the microfluidic chip 5000 at once to improve the operating efficiency of the device, the number of channels of the pipette is preferably consistent with the number of inlets of the microfluidic chip 5000, that is, in this application, the pipette is preferably a multi-channel pipette. Specifically, as Figure 3 As shown, an 8-channel pipette is used in this embodiment.

[0124] Regarding the structure of the pipette, it generally includes a control button for pipetting, and also includes a tip removal button for replacing the tip. Regarding other specific structures of the pipette, since the pipette is a mature commercial product, it will not be repeated in this application.

[0125] Furthermore, the pipetting module 2000 also includes: a second motion unit, which can drive the control button of the pipette to perform pipetting; and / or a third motion unit, which can drive the tip removal button of the pipette to remove the tip.

[0126] Thus, the pipetting module 2000 can realize automatic pipetting through the second motion unit and automatic replacement of the gun tip through the third motion unit.

[0127] Regarding pipetting, when the pipette needs to aspirate, the second motion unit pushes the control button of the pipette, and then the pipette aspirates by automatically rebounding or pulling back the second motion unit; when the pipette needs to discharge, the second motion unit pushes the control button of the pipette to discharge.

[0128] Regarding the replacement of the gun tip, the gun tip and the recycling area can be pre-set on the bottom plate. When the pipette gun needs to install the gun tip, the motion module drives the pipette gun to move to the upper part of the gun tip and presses down to install the gun tip; when the pipetting is completed and the gun tip needs to be replaced, the motion module drives the pipette gun to move to the recycling area, and the third motion unit pushes the pipette gun's tip removal button to remove the gun tip. In this way, the gun tip can be replaced automatically to prevent residual liquid in the gun tip from affecting the accuracy of the reaction.

[0129] The present application has no specific restrictions on the second motion unit and the third motion unit, as long as they can achieve push-pull, for example, a lead screw, an electric push rod, a cylinder, etc. In this embodiment, it is a lead screw.

[0130] In one embodiment, Figure 1 to Figure 3 As shown, the motion module includes:

[0131] A fixing component 3100 (specifically a mechanical arm in this embodiment);

[0132] A horizontal motion component, wherein the horizontal motion component can drive the fixed component to move in a horizontal direction;

[0133] A vertical motion component, wherein the vertical motion component, the pressure supply module and the pipetting module are arranged on the fixed component, and the vertical motion component can independently drive the pressure supply module and the pipetting module to move in a vertical direction (such as the z direction mentioned above).

[0134] Thus, the pressure supply module 1000 and the pipetting module 2000 arranged on the fixed component can be driven to move in the horizontal direction through the horizontal motion component, and the pressure supply module 1000 and the pipetting module 2000 can be driven to move in the vertical direction through the vertical motion component.

[0135] In one embodiment, Figure 1 As shown, the horizontal motion component includes: a first horizontal motion unit, which can drive the fixed component to move in a first horizontal direction (such as the x direction mentioned above); and a second horizontal motion unit, which can drive the first horizontal motion unit to move in a second horizontal direction (such as the y direction mentioned above).

[0136] That is, the horizontal motion component in this embodiment can drive the pressure supply module 1000 and the pipetting module 2000 arranged in the fixed component to move in the entire horizontal plane direction (the horizontal plane direction formed by the first horizontal direction and the second horizontal direction) through the first horizontal motion unit and the second horizontal motion unit. Combined with the above-mentioned vertical motion component, the motion module can drive the pressure supply module 1000 and the pipetting module to perform translational motion along three directions in space.

[0137] like Figure 1 As shown, regarding the first horizontal motion unit, it includes: a first guide rail 3211, the first guide rail 3211 is arranged along the first horizontal direction; a fourth motion unit, the fourth motion unit can drive the fixed component to slide along the first guide rail. Regarding the second horizontal motion unit, it includes: a second guide rail 3221 (in this embodiment, two second guide rails 3221 arranged in parallel), the second guide rail 3221 is arranged along the second horizontal direction; a fifth motion unit, the fifth motion unit can drive the first horizontal motion unit to slide along the second guide rail. Thus, the horizontal motion component drives the pressure supply module 1000 and the pipetting module 2000 arranged on the vertical motion component to move in the entire horizontal plane direction (the horizontal plane direction formed by the first horizontal direction and the second horizontal direction) through the first horizontal motion unit and the second horizontal motion unit.

[0138] The present application has no specific limitation on the fourth motion unit and the fifth motion unit, and examples thereof include lead screws, electric push rods, etc., which are all lead screws in the present embodiment.

[0139] In one embodiment, the vertical motion assembly includes: a first vertical motion unit, which can drive the pressure supply module to move in the vertical direction; and a second vertical motion unit, which can drive the liquid transfer module to move in the vertical direction. Thus, the vertical motion assembly can independently drive the pressure supply module and the liquid transfer module to move in the vertical direction through the first vertical motion unit and the second vertical motion unit, so that the pressure supply module independently presses the upper part of the microfluidic chip 5000 to facilitate the synthesis of liquid nanoparticles, and the liquid transfer module independently moves up and down to facilitate liquid transfer and replacement of gun tips, etc.

[0140] The present application has no specific limitation on the first vertical motion unit and the second vertical motion unit, and examples thereof include a lead screw, an electric push rod, a cylinder, etc., which are all lead screws in the present embodiment.

[0141] At present, the above-mentioned device developed by the applicant (see Figures 1 to 9 ), has experimentally verified the automated nanoparticle formula screening based on microfluidic technology. It is only necessary to place the raw materials (such as aqueous phase, organic phase liquid) and consumables (such as gun tips, sealing pads) required for nanoparticle synthesis, and collect containers (such as well plates / centrifuge tubes), and configure the reagents in the control system of the device (such as the corresponding software of the above control components) and set the synthesis parameters (such as the total flow rate and flow rate ratio of each phase reagent, synthesis volume), and up to 96 formulas can be automatically screened within 1 hour, greatly shortening the time and manpower required for formula / drug screening. At the same time, the minimum volume of each sample synthesis can be as low as 20μl, which greatly reduces the consumption of samples and saves a lot of material costs.

[0142] Use the device provided by this application (see Figures 1 to 9 ), the synthesis of nanoparticles specifically includes the following process:

[0143] 1. Preparation of consumables / reagents

[0144] 1) Place the containers (96-well plates and reservoirs for storing liquid phases (such as aqueous phase and organic phase) and the collection containers (96-well plates for collection) on the corresponding bases and then on the bottom plate; place the microfluidic chip on the chip tray and then on the bottom plate; place other consumables (pipette tips, sealing pads) on the base and then on the bottom plate of the device;

[0145] 4) Set reagent library preparation, reagent library configuration and nanoparticle synthesis parameters (including total flow rate, flow rate ratio of different reagents and synthesis volume) through software.

[0146] 2. Reagent library preparation (optional)

[0147] The reagents in the wells of different 96-well plates for storage or in the liquid storage tanks are mixed and diluted by using a pipette to obtain reagents for synthesizing nanoparticles mixed with different raw materials or with different concentrations.

[0148] If reagent library preparation is not required, the pre-prepared reagents for nanoparticle synthesis can be placed in the corresponding 96-well plate or liquid reservoir for storage and the reagent library can be directly configured in the software.

[0149] 3. Nanoparticle synthesis

[0150] The microfluidic chip contains four of the above reaction combinations. The four reaction combinations on the chip can be pressurized at the same time to achieve the synthesis of four samples.

[0151] 1) Pick up the pipette tip with a multichannel pipette and move it to the top of the online dilution reservoir, and draw 100 μl × 4 online dilutions (taking 1:1 dilution as an example);

[0152] 2) Move the pipette to the top of the microfluidic chip outlet and place the online dilution solution into the four outlets;

[0153] 3) Replace the pipette tip;

[0154] 4) Move the pipette to the top of the 96-well storage plate and draw 75 μl × 4 aqueous phase reagent (nucleic acid solution);

[0155] 5) Move the pipette to above the aqueous reagent inlet of the microfluidic chip and place the aqueous reagent into the four aqueous reagent inlets;

[0156] 6) Replace the pipette tip;

[0157] 7) Move the pipette to the top of the 96-well storage plate and draw 25 μl × 4 organic phase reagent (lipid solution);

[0158] 8) Move the pipette to above the inlet of the organic phase reagent of the microfluidic chip, and place the organic phase reagent into the four inlets of the organic phase reagent;

[0159] 9) Remove the pipette tip;

[0160] 10) The pressure plate assembly moves to the top of the gasket storage base, and the gasket fixing assembly picks up a new gasket;

[0161] 11) The pressing plate assembly moves to the top of the microfluidic chip containing the reagent, the pressing plate assembly descends, and the pressing plate body contacts and seals the microfluidic chip through the sealing gasket;

[0162] 12) After the pressure supply module is pressurized to a certain pressure, the first control valve and the second control valve are switched simultaneously to supply gas to the microfluidic chip, and the pressure is used to simultaneously drive the aqueous phase and organic phase reagents in the inlet of the microfluidic chip to enter the flow channel in the microfluidic chip at a certain flow rate for mixing, synthesizing 100 μl of nanoparticle solution and driving the nanoparticle solution into the outlet of the microfluidic chip;

[0163] 13) The first control valve and the second control valve are switched to connect the inlet of the microfluidic chip to the atmosphere, and the reservoir is depressurized so that the reagent no longer flows, and the synthesis of nanoparticles is completed;

[0164] 14) The pressing plate assembly together with the sealing gasket rises and leaves the microfluidic chip;

[0165] 15) Remove the platen assembly and remove the sealing gasket to the consumables recovery room;

[0166] 16) Pick up the pipette tip with a pipette, move it to the top of the microfluidic chip outlet, and draw 200 μl of the synthesized nanoparticle solution × 4;

[0167] 17) Move the pipette to the top of the 96-well plate for collection and place 200 μl×4 nanoparticle solution into the corresponding wells;

[0168] 18) Remove the pipette tip from the pipette and place it in the consumables recovery room (i.e. the above-mentioned recovery area);

[0169] 19) Repeat steps 1-18 to synthesize the next group (4 samples);

[0170] 20) When all microfluidic chips on a microfluidic chip base are synthesized, the pressure plate module moves to the next microfluidic chip base with microfluidic chips and repeats steps 1-18 to synthesize the sample.

[0171] 21) The synthesis is completed.

[0172] Although the embodiments of the present application are described above, the present application is not limited to the above specific embodiments and application fields, and the above specific embodiments are merely illustrative and instructive, rather than restrictive. A person of ordinary skill in the art can make many forms under the guidance of this specification and without departing from the scope of protection of the claims of the present application, all of which belong to the scope of protection claimed in the present application.

Claims

1. A device for automatically synthesizing nanoparticles, wherein: include: A pressure supply module, wherein the pressure supply module is provided with one or more gas outlets corresponding to the inlet of the microfluidic chip, so as to be able to supply gas to the inlet of the microfluidic chip or stop supplying gas through the gas outlets; A pipetting module, which is capable of pipetting liquid toward an inlet of the microfluidic chip; A motion module, wherein the motion module can drive the pressure supply module and the pipetting module to move in a horizontal direction and in a vertical direction.

2. The device according to claim 1, wherein: The pressure supply module comprises: Platen assembly; An air supply component is provided, wherein the air supply component is capable of supplying air to the air outlet.

3. The device as claimed in claim 2, wherein: The pressure plate assembly comprises: Platen body; The air inlet and the air outlet are both arranged on the pressing plate body; A gas flow channel is arranged in the pressing plate body to connect the gas inlet and the gas outlet.

4. The device as claimed in claim 3, wherein: The air inlet comprises at least a first air inlet and a second air inlet; The air outlet comprises at least a first air outlet and a second air outlet; The gas flow channel at least includes a first flow channel and a second flow channel; in, The first gas outlet is used to supply gas to the first phase inlet of the microfluidic chip or stop supplying gas; The second gas outlet is used to supply gas to the second phase inlet of the microfluidic chip or stop supplying gas; The first flow channel is used to connect the first air inlet and the first air outlet; The second flow channel is used to connect the second air inlet and the second air outlet.

5. The device according to claim 4, wherein: The pressure plate assembly also includes: The first control valve and the second control valve each include at least a first interface, a second interface, and a third interface, and can be switched between the first interface being connected to the second interface and the first interface being connected to the third interface; A first connection port group and a second connection port group, both of which are arranged on the pressing plate body and both of which include at least a first connection port, a second connection port, and a third connection port; in, The first interface of the first control valve can be connected to the first gas outlet through the first connection port of the first connection port group and the gas flow channel, the second interface of the first control valve can be connected to the first gas inlet through the second connection port of the first connection port group and the gas flow channel, and the third interface of the first control valve is in communication with the atmosphere; The first interface of the second control valve can be connected to the second air outlet through the first connection port of the second connection port group and the gas flow channel, the second interface of the second control valve can be connected to the second air inlet through the second connection port of the second connection port group and the gas flow channel, and the third interface of the second control valve is connected to the atmosphere.

6. The device according to claim 5, wherein: The gas supply assembly includes at least: A first gas storage tank and a second gas storage tank, wherein an inlet of the first gas storage tank and an inlet of the second gas storage tank are independently connected to a gas source; in, The outlet of the first gas storage tank is connected to the first gas inlet; The outlet of the second air storage tank is connected to the second air inlet.

7. The device according to claim 6, wherein: The pressure supply module also includes: A control component is provided, wherein the control component independently controls the gas source to supply gas to the first gas storage tank and the second gas storage tank.

8. The device of claim 3, wherein: The pressure supply module also includes: A sealing gasket is provided with a through hole corresponding to the air outlet, and the sealing gasket is arranged on one side of the pressure plate body where the air outlet is arranged, so as to seal when the air outlet supplies air to the inlet of the microfluidic chip or stops supplying air.

9. The device of claim 8, wherein: A filter element is arranged on a through hole of at least one of the sealing pads corresponding to the air outlet.

10. The device of claim 8, wherein: The pressure supply module also includes: A sealing gasket fixing assembly is provided, wherein the sealing gasket is fixed to a side of the pressing plate body where the air outlet is arranged and the sealing gasket is detached from the pressing plate body.

11. The device of claim 10, wherein: The pressing plate body is provided with a magnetic suction channel; The sealing pad fixing assembly is located on the opposite side of the pressure plate body where the air outlet is arranged; The gasket retaining assembly includes: Magnetic unit; The first moving unit can drive the magnetic unit to pass through the magnetic channel to magnetically attract the sealing gasket, and can drive the magnetic unit away from the sealing gasket to make the sealing gasket detach from the pressure plate body.

12. The device of claim 11, wherein: The first motion unit comprises: A magnetic unit fixing plate, wherein the magnetic unit fixing plate fixes the magnetic unit; A first motor, wherein the first motor can drive the magnetic attraction unit fixing plate to move closer to or away from the pressing plate body.

13. The device of claim 1, wherein: The pipetting module comprises: A pipetting unit is provided, wherein the pipetting unit is capable of sucking in and discharging liquid.

14. The device of claim 13, wherein: The pipetting unit is a pipette, and the pipette is a single-channel pipette or a multi-channel pipette; The pipetting module also includes: a second motion unit, the second motion unit being capable of driving a control button of the pipette gun to perform pipetting; The third motion unit can drive the tip removal button of the pipette to remove the pipette tip.

15. The device of claim 14, wherein: The motion module comprises: Fixing components; A horizontal motion component, wherein the horizontal motion component can drive the fixed component to move in a horizontal direction; A vertical motion component, wherein the vertical motion component, the pressure supply module and the pipetting module are arranged on the fixed component, and the vertical motion component can independently drive the pressure supply module and the pipetting module to move in the vertical direction.

16. The device of claim 15, wherein: The horizontal motion assembly comprises: A first horizontal motion unit, wherein the first horizontal motion unit can drive the fixing assembly to move in a first horizontal direction; The second horizontal motion unit is capable of driving the first horizontal motion unit to move in a second horizontal direction.

17. The device of claim 16, wherein: The first horizontal motion unit comprises: a first guide rail, the first guide rail being arranged along a first horizontal direction, a fourth motion unit, wherein the fourth motion unit is capable of driving the fixing assembly to slide along the first guide rail; and / or, The second horizontal motion unit comprises: a second guide rail, the second guide rail being arranged along a second horizontal direction, A fifth motion unit, wherein the fifth motion unit can drive the first horizontal motion unit to slide along the second guide rail.

18. The device of claim 15, wherein: The vertical motion assembly comprises: A first vertical motion unit, wherein the first vertical motion unit can drive the pressure supply module to move in a vertical direction; A second vertical motion unit, wherein the second vertical motion unit can drive the pipetting module to move in a vertical direction.

19. The device of claim 1, wherein: Also includes: A box module, wherein the box module accommodates the pressure supply module, the pipetting module and the motion module; and, An air filter module is arranged on the box module.

Images