Detection device for enzyme-linked immunosorbent assay (ELISA) enzyme

Abstract

The utility model provides an ELISA enzyme detection device, which comprises a box body, a fixed bottom plate, a fixed plate, a clamping mechanism and a test tube, the box body is internally provided with an accommodating cavity, the fixed bottom plate and the fixed plate are both connected in the accommodating cavity, the fixed bottom plate and the fixed plate are arranged at an interval along the vertical direction, the fixed bottom plate is located below the fixed plate, and the test tube is arranged in the accommodating cavity. The clamping mechanism is connected to the fixing plate, the clamping mechanism is provided with a clamping space, a test tube is detachably connected in the clamping space, the test tube is placed in the box body, dust or bacteria in the air are prevented from entering the test tube, and meanwhile, the test tube is clamped by the clamping mechanism, so that the stability of the test tube is kept.

Description

Technical Field

[0001] The present application belongs to the technical field of ELISA enzyme detection, and more specifically, relates to an ELISA enzyme detection device. Background Art

[0002] ELISA, or Enzyme-Linked Immunosorbent Assay, is a special reagent analysis method, a new type of immunoassay technology developed based on immunoenzyme technology. Its basic method is to adsorb known antigens or antibodies on the surface of a solid phase carrier (such as a polystyrene microplate), allow the enzyme-labeled antigen-antibody reaction to proceed on the solid phase surface, and remove the free components in the liquid phase by washing.

[0003] In ELISA testing, the selection of enzymes is crucial. They must be of high purity, high conversion rate of catalytic reactions, strong specificity, stable properties, abundant sources, and inexpensive. The prepared enzyme-labeled antibodies or antigens must be stable in nature and continue to retain their active parts and catalytic ability. The most commonly used enzymes include horseradish peroxidase (HRP) and alkaline phosphatase (AP) extracted from bovine intestinal mucosa or Escherichia coli.

[0004] When conducting an experiment, you first need to select the appropriate ELISA kit and enzyme-labeled plate, and prepare the enzyme-labeled antibody, substrate solution, and stop solution. Then, the entire detection process is completed through steps such as coating, adding samples, incubation, washing, color development, and termination.

[0005] In order to facilitate detection, test tubes are usually used to hold substrate solutions. At present, test tubes containing substrate solutions are usually placed on test tube racks. This causes the following problems: since the test tubes placed on the test tube racks are open, dust and bacteria in the air can easily enter the test tubes, thereby affecting the test results. Summary of the invention

[0006] The purpose of the embodiments of the present application is to provide an ELISA enzyme detection device to solve the technical problems existing in the prior art.

[0007] To achieve the above-mentioned purpose, the technical solution adopted in the present application is: to provide an ELISA enzyme detection device, comprising:

[0008] A box body, with a containing cavity inside;

[0009] A fixed bottom plate and a fixed plate, both of which are connected to the accommodating cavity, the fixed bottom plate is spaced apart from the fixed plate in the vertical direction, and the fixed bottom plate is located below the fixed plate;

[0010] A clamping mechanism connected to the fixing plate, wherein the clamping mechanism is provided with a clamping space;

[0011] The test tube is detachably connected in the clamping space.

[0012] Optionally, the clamping mechanism comprises:

[0013] The first connecting plate and the second connecting plate are both fixedly connected to the fixing plate, and the first connecting plate and the second connecting plate are arranged opposite to each other;

[0014] A first clamping plate and a second clamping plate, wherein the first clamping plate is slidably connected to the first connecting plate, and the first clamping plate and the first connecting plate are provided with a first spring; the second clamping plate is slidably connected to the second connecting plate, and the second clamping plate and the second connecting plate are provided with a second spring;

[0015] The first clamping plate and the second clamping plate form the clamping space.

[0016] Optionally, the clamping mechanism further comprises:

[0017] The third connecting plate and the fourth connecting plate are both fixedly connected to the fixed bottom plate, and the third connecting plate and the fourth connecting plate are arranged opposite to each other;

[0018] The third clamping plate and the fourth clamping plate, the third clamping plate is slidably connected to the third connecting plate, and the third clamping plate and the third connecting plate are provided with a third spring; the fourth clamping plate is slidably connected to the fourth connecting plate, and the fourth clamping plate and the fourth connecting plate are provided with a fourth spring.

[0019] Optionally, the clamping mechanism further comprises:

[0020] A placing rack is detachably connected to the fixed bottom plate and the fixed plate, and is used to support the test tube.

[0021] Optionally, a pull rod is provided on the placement rack.

[0022] Optionally, a plug hole is provided on the fixed bottom plate, and the bottom end of the pull rod is plugged into the plug hole.

[0023] The beneficial effect of the ELISA enzyme detection device provided by the present application is that: compared with the prior art, the ELISA enzyme detection device of the present application includes a box body, a fixed bottom plate, a fixed plate, a clamping mechanism and a test tube, the box body is provided with a accommodating cavity, the fixed bottom plate and the fixed plate are both connected to the accommodating cavity, the fixed bottom plate is arranged at intervals along the vertical direction of the fixed plate, and the fixed bottom plate is located below the fixed plate, the clamping mechanism is connected to the fixed plate, the clamping mechanism is provided with a clamping space, the test tube is detachably connected to the clamping space, and by placing the test tube in the box body, dust or bacteria in the air can be prevented from entering the test tube, and at the same time, the test tube is clamped by the clamping mechanism to maintain the stability of the test tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0025] Figure 1 A schematic diagram of the structure of an ELISA enzyme detection device provided in an embodiment of the present application;

[0026] Figure 2 Schematic diagram of the clamping structure provided in the embodiment of the present application Figure 1 ;

[0027] Figure 3 Schematic diagram of the clamping structure provided in the embodiment of the present application Figure 2 ;

[0028] Figure 4 Schematic diagram of the clamping structure provided in the embodiment of the present application Figure 3 .

[0029] Among them, the reference numerals in the figure are:

[0030] 1. Box body; 2. Fixed bottom plate; 3. Fixed plate; 4. Test tube; 5. Accommodating chamber; 6. First connecting plate; 7. Second connecting plate; 8. First clamping plate; 9. Second clamping plate; 10. Third connecting plate; 11. Fourth connecting plate; 12. Third clamping plate; 13. Fourth clamping plate; 14. First spring; 15. Second spring; 16. Third spring; 17. Fourth spring; 18. Placement rack; 19. Pull rod; 20. Plug hole. DETAILED DESCRIPTION

[0031] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present application more clearly understood, the present application is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application.

[0032] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

[0033] It should be understood that the terms "length", "width", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0034] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0035] Please also read Figures 1 to 4 , the ELISA enzyme detection device provided in the embodiments of the present application is now described.

[0036] This specific embodiment provides an ELISA enzyme detection device, which is convenient for detecting the activity of the ELISA enzyme.

[0037] The ELISA enzyme detection device comprises a box body 1 , a fixed bottom plate 2 , a fixed plate 3 , a clamping mechanism and a test tube 4 .

[0038] The box body 1 is provided with a receiving cavity 5 for receiving and fixing other components.

[0039] The fixed bottom plate 2 and the fixed plate 3 are both connected to the accommodating cavity 5. The fixed bottom plate 2 is located below the fixed plate 3, and the two are spaced apart in the vertical direction.

[0040] The clamping mechanism is connected to the fixing plate 3, and a clamping space is provided on the clamping mechanism, and the clamping space is used to clamp the test tube 4. The clamping mechanism includes a first connecting plate 6, a second connecting plate 7, a first clamping plate 8, a second clamping plate 9, a third connecting plate 10, a fourth connecting plate 11, a third clamping plate 12 and a fourth clamping plate 13.

[0041] The first connecting plate 6 and the second connecting plate 7 are both fixedly connected to the fixing plate 3 and are arranged opposite to each other. The first clamping plate 8 is slidably connected to the first connecting plate 6, and a first spring 14 is provided between the first clamping plate 8 and the first connecting plate 6. The second clamping plate 9 is slidably connected to the second connecting plate 7, and a second spring 15 is provided between the second clamping plate 9 and the second connecting plate 7. The first clamping plate 8 and the second clamping plate 9 are arranged opposite to each other to form a clamping space. The first clamping plate 8 and the second clamping plate 9 clamp the test tube 4 under the action of the elastic force of the first spring 14 and the second spring 15, respectively.

[0042] The third connecting plate 10 and the fourth connecting plate 11 are both fixedly connected to the fixed plate 3 and are arranged opposite to each other. The third clamping plate 12 is slidably connected to the third connecting plate 10, and a third spring 16 is arranged between the third clamping plate 12 and the third connecting plate 10. The fourth clamping plate 13 is slidably connected to the fourth connecting plate 11, and a fourth spring 17 is arranged between the fourth clamping plate 13 and the fourth connecting plate 11. The third clamping plate 12 and the fourth clamping plate 13 are arranged opposite to each other to form a clamping space. The third clamping plate 12 and the fourth clamping plate 13 clamp the lower end of the test tube 4 under the action of the elastic force of the third spring 16 and the fourth spring 17, respectively, to ensure the stability of the test tube 4.

[0043] In addition, the clamping mechanism further includes a placing rack 18, which is detachably connected between the fixed bottom plate 2 and the fixed plate 3. The placement of the placing rack 18 provides additional support for the test tube 4 while clamping, further improving stability. A pull rod 19 is also provided on the placing rack 18 to facilitate the operation of the operator, so that the operator can drive the test tube 4 to move relative to the clamping mechanism in the vertical direction through the placing rack 18.

[0044] The fixed bottom plate 2 is provided with an inserting hole 20 , and the bottom end of the pull rod 19 is inserted into the inserting hole 20 , so that the pull rod 19 is firmly connected to the fixed bottom plate 2 and is not easy to fall off.

[0045] In actual use, the operator can place the test tube 4 on the placement rack 18, and make the first clamping plate 8 and the second clamping plate 9 clamp the upper end of the test tube 4. At the same time, the third clamping plate 12 and the fourth clamping plate 13 also clamp the test tube 4 from below. In this way, the test tube 4 is firmly clamped in the device and is not easy to shake or fall off. When performing ELISA enzyme detection, the device can keep the test tube 4 stable, thereby improving the accuracy and efficiency of the detection.

[0046] Compared with the prior art, the ELISA enzyme detection device of the present application includes a box body 1, a fixed base plate 2, a fixed plate 3, a clamping mechanism and a test tube 4. A accommodating chamber 5 is provided inside the box body 1. The fixed base plate 2 and the fixed plate 3 are both connected to the accommodating chamber 5. The fixed base plate 2 and the fixed plate 3 are spaced apart in the vertical direction, and the fixed base plate 2 is located below the fixed plate 3. The clamping mechanism is connected to the fixed plate 3. The clamping mechanism is provided with a clamping space. The test tube 4 is detachably connected to the clamping space. By placing the test tube 4 in the box body 1, dust or bacteria in the air can be prevented from entering the test tube 4. At the same time, the test tube 4 is clamped by the clamping mechanism to maintain the stability of the test tube 4.

[0047] The above are only preferred embodiments of the present application and are not intended to limit the present application. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. An ELISA enzyme detection device, characterized in that: A box body, with a containing cavity inside; A fixed bottom plate and a fixed plate, both of which are connected to the accommodating cavity, the fixed bottom plate is spaced apart from the fixed plate in the vertical direction, and the fixed bottom plate is located below the fixed plate; A clamping mechanism connected to the fixing plate, wherein the clamping mechanism is provided with a clamping space; A test tube, detachably connected to the clamping space; The clamping mechanism comprises: The first connecting plate and the second connecting plate are both fixedly connected to the fixing plate, and the first connecting plate and the second connecting plate are arranged opposite to each other; A first clamping plate and a second clamping plate, wherein the first clamping plate is slidably connected to the first connecting plate, and the first clamping plate and the first connecting plate are provided with a first spring; the second clamping plate is slidably connected to the second connecting plate, and the second clamping plate and the second connecting plate are provided with a second spring; The first clamping plate and the second clamping plate form the clamping space.

2. The ELISA enzyme detection device according to claim 1, characterized in that: The clamping mechanism also includes: The third connecting plate and the fourth connecting plate are both connected to the fixed bottom plate, and the third connecting plate and the fourth connecting plate are arranged opposite to each other; The third clamping plate and the fourth clamping plate, the third clamping plate is slidably connected to the third connecting plate, and the third clamping plate and the third connecting plate are provided with a third spring; the fourth clamping plate is slidably connected to the fourth connecting plate, and the fourth clamping plate and the fourth connecting plate are provided with a fourth spring.

3. The ELISA enzyme detection device according to claim 2, characterized in that: The clamping mechanism also includes: A placing rack is detachably connected to the fixed bottom plate and the fixed plate, and is used to support the test tube.

4. The ELISA enzyme detection device according to claim 3, characterized in that: A pull rod is arranged on the placement rack.

5. The ELISA enzyme detection device according to claim 4, characterized in that: The fixed bottom plate is provided with an inserting hole, and the bottom end of the pull rod is inserted into the inserting hole.

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