Automatic sampling instrument

Abstract

The utility model aims to develop an automatic sampling instrument which can replace manual blood sampling and simplify pharmacokinetic research. The main accessories comprise waste liquid tanks (1-1 and 1-2); pipe pressing valves (2-1 to 2-5); a semiconductor refrigerating chamber (4); a collection disc (5); a liquid separation dropper (6); needle heads (7-1, 7-2); a peristaltic pump (8); electromagnetic switching valves (9-1, 9-2); normal saline bottles (10-1, 10-2); an injection pump (11-1, 11-2); a polyurethane pipe (12-1 to 12-5); silica gel tubes (13-1 to 13-6); a blood sensor (14-1, 14-2); a four-way joint (15); a three-way joint (16); a reducer union (17); a three-way solenoid valve (18); and a component control and data collection system (19). The instrument can be used for regularly and quantitatively collecting blood, is accurate in test result and small in error range, can be used for carrying out long-time and multi-frequency collection, and can be widely applied to research and development of novel medicines.

Description

Technical Field

[0001] The invention relates to the fields of drug metabolism dynamics and medical animal experiments, and is used for accurately and quickly collecting animal blood. Background Art

[0002] In the field of biological sciences, especially in the process of drug development, experimental researchers develop new drugs by injecting drugs or viruses into experimental animals and then collecting blood samples from experimental animals for analysis, which usually involves necessary clinical animal experiments. In the process of clinical animal experiments, blood collection from experimental animals is a key technology.

[0003] At present, most drug research departments mainly use manual blood collection when conducting animal experiments. Common manual blood collection methods include tail vein blood collection, cardiac puncture blood collection, neck vein or artery blood collection, ear vein blood collection, hind limb vein blood collection, eyeball blood collection, etc. Although manual blood collection methods are widely used in animal experimental research, they have the following disadvantages: the manual blood collection process may cause great stress and pain to animals, especially in long-term studies that require repeated blood collection. Stress not only affects animal welfare, but may also interfere with experimental results, such as affecting physiological and biochemical parameters; manual blood collection technology requires operators to have high skills and experience. Improper technical operation can easily cause animal injuries or blood collection failures. There may be differences in the operations between different operators or the same operator at different times, affecting the consistency of blood collection and the repeatability of the experiment; during manual blood collection, the risk of sample contamination is high, which may affect the results of subsequent biological analysis; manual blood collection is usually more time-consuming than automated blood collection systems, especially when dealing with a large number of animals, and is inefficient. Considering that the experimental process not only consumes a lot of human resources, but the accuracy of the experiment also depends on the operator's skill level, professional knowledge and work attitude, it is particularly important to develop automated blood collection equipment.

[0004] Building an automatic blood collection instrument for experimental animals requires professional mechanical design and biomedical knowledge. At present, there are some related devices for automatic blood collection, such as: Dong Chao et al., Animal Automatic Sampling and Dosing System, Chinese Patent 201210193206.1; Wan Huayin et al., A Biosafety Quality Control System for Animal Plasma, Serum or Blood, Chinese Patent 201610192745.1; Lu Yangzhou, An Automated Blood Collection System, Chinese Patent 201811090662.7; Chen Yong, Animal Blood Collection Device, Collection System and Method, Chinese Patent 201910657235.0; Xue Gang et al., An Animal Blood Collection System, Chinese Patent 201910657235.0; Patent 202010368298.7; Chen Yunxi et al., a new automatic blood collection instrument and blood collection method design, Chinese patent 202110016253.8; Yuan Wei et al., an intelligent multi-micro sample pretreatment platform and its pretreatment method, Chinese patent 202210279859.5. Although simple automatic blood collection can be achieved, the collection accuracy is insufficient and can only meet the collection requirements at the milliliter level, and cannot achieve the collection accuracy of microliters and below; and these devices are not equipped with sensitive and effective blood sensors and cold storage rooms that meet blood storage standards.

[0005] The blood collection methods and conditions for experimental mice of different sizes are also different, which also requires the automatic sampler to be upgraded in time to adapt to different test standards to meet the needs of more users. In addition, in addition to collecting blood from experimental mice, the automatic sampler can also collect blood from other animals (rabbits, chickens, dogs). Summary of the invention

[0006] In view of the above problems, the purpose of the present invention is to develop an automatic sampler to achieve accurate and rapid blood drawing and better meet industry standards and scientific research needs.

[0007] The schematic diagram of the automatic sampler is shown in Figure 1As shown, the main accessories include a waste liquid tank (1-1, 1-2); a first compression pipe valve (2-1), a second compression pipe valve (2-2), a third compression pipe valve (2-3), a fourth compression pipe valve (2-4), and a fifth compression pipe valve (2-5); a semiconductor cold storage room (4); a collection plate (5); a liquid dropper (6); a needle (7-1, 7-2); a peristaltic pump (8); an electromagnetic switching valve (9-1, 9-2); a physiological saline bottle (10-1, 10-2); a syringe pump (11-1, 11-2); a first polyurethane tube (12-1), a second polyurethane tube ( 12-2), a third polyurethane tube (12-3), a fourth polyurethane tube (12-4), a fifth polyurethane tube (12-5); a first silicone tube (13-1), a second silicone tube (13-2), a third silicone tube (13-3), a fourth silicone tube (13-4), a fifth silicone tube (13-5), a sixth silicone tube (13-6); a blood sensor (14-1, 14-2); a four-way connector (15); a three-way connector (16); a reducer (17); a three-way solenoid valve (18); and a component control and data collection system (19).

[0008] The injection pump (11-1, 11-2) and electromagnetic switching valve (9-1, 9-2) are characterized in that the valve and the pump are integrated together, which can save joints and pipelines, avoid interference from bubbles generated in the pipeline, and save installation space.

[0009] The injection pump (11-1, 11-2) realizes a precise and stable liquid delivery process through a stepping motor and its driver, and a pull rod that can reciprocate up and down.

[0010] One end of the injection pump (11-1) and the electromagnetic switching valve (9-1) is connected to the physiological saline bottle (10-1) through the first polyurethane tube (12-1), and the other end is connected to the four-way joint (15) through the second polyurethane tube (12-2), the reducer (17), and the third silicone tube (13-3). One end of the injection pump (11-2) and the electromagnetic switching valve (9-2) is connected to the air and the physiological saline bottle (10-2) through the three-way electromagnetic valve (18) and the fourth polyurethane tube (12-4), and the other end is connected to the three-way joint (16) through the third polyurethane tube (12-3).

[0011] The first compression pipe valve (2-1) is connected between the waste liquid tank (1-1) and the four-way joint (15) through a first silicone tube (13-1); the second compression pipe valve (2-2) is connected between the needle head (7-1) and the four-way joint (15) through a second silicone tube (13-2); the third compression pipe valve (2-3) is connected between the three-way switching valve and the four-way joint (15) through a third silicone tube (13-3), a reducer (17), and a second polyurethane tube (12-2); the fourth compression pipe valve (2-4) is connected between the four-way joint (15) and the three-way joint (16) through a fourth silicone tube (13-4); and the fifth compression pipe valve (2-5) is connected between the three-way joint (16) and the liquid dropper (6) through a fifth silicone tube (13-5).

[0012] The blood sensor (14-1, 14-2) is characterized in that two metal conductivity probes are connected to the second silicone tube (13-2) according to the conductivity characteristics of blood, and accurate and sensitive response to blood is achieved by detecting the conductivity change when blood flows through the second silicone tube (13-2).

[0013] The blood sampling method of the present invention comprises (assuming that all the compression tube valves are closed before blood sampling): opening the second compression tube valve (2-2) and the third compression tube valve (2-3), and the injection pump (11-1) extracts blood from a mouse (3) through the second polyurethane tube (12-2), the reducer (17), the third silicone tube (13-3), and the needle (7-1); when the blood gradually enters the pipeline of the four-way connector (15), the blood sensors (14-1, 14-2) detect the blood, and at this time the injection pump (11-1) stops drawing blood. The fourth compression tube valve (2-4) is opened, and the injection pump (11-2) starts to draw blood, allowing the blood to pass through the fourth silicone tube (13-4) and the three-way connector (16) and enter the third polyurethane tube (12-3); after the set blood volume is drawn, the fourth compression tube valve (2-4) is closed, the fifth compression tube valve (2-5) is opened, and the blood is sent to the liquid dropper (6) through the injection pump (11-2) and the fifth silicone tube (13-5), and finally dripped into the collection plate (5) in the semiconductor cold storage room (4) through the needle (7-2).

[0014] The semiconductor cold storage room (4) temporarily refrigerates and preserves the blood. Its characteristic is that the working principle is based on the Peltier effect: after the semiconductor refrigeration piece is connected to the DC power supply, the electrons start from the negative electrode, first pass through the P-type semiconductor to absorb heat, and then go to the N-type semiconductor to release the heat. Every time it passes through an NP module, heat is sent from one side to the other side, causing a temperature difference, thereby forming a cold and hot end; the cold end is attached to the aluminum block of the main body of the cold storage room, and the hot end dissipates heat through the fan; the cooling capacity is controlled by the power of the semiconductor refrigeration piece. Compared with traditional compressor refrigeration, the semiconductor cold storage room (4) has the advantages of small size, low power, and portability.

[0015] The component control and data collection system (19) includes a microcomputer, a mainboard, a single-chip microcomputer, etc., which can realize automatic control of the injection pump speed and the peristaltic pump speed, as well as digital collection of parameters such as storage temperature and liquid flow rate; and the user can set the test conditions and parameters according to the test requirements.

[0016] The liquid pipeline used in the automatic sampler of the present invention should be made of plastic material as much as possible, and the pipelines passing through the first compression pipe valve (2-1), the second compression pipe valve (2-2), the third compression pipe valve (2-3), the fourth compression pipe valve (2-4), and the fifth compression pipe valve (2-5) should be made of softer and thinner materials to facilitate the opening and closing of the valves. Therefore, the pipelines involving the first compression pipe valve (2-1), the second compression pipe valve (2-2), the third compression pipe valve (2-3), the fourth compression pipe valve (2-4), and the fifth compression pipe valve (2-5) are all made of silicone material with an inner diameter of 0.5mm; the connecting pipeline of the liquid dropper (6) is relatively thin, and a silicone tube connection should also be selected. The remaining pipeline materials are polyurethane tubes with an inner diameter of 0.5-1.6mm. In order to save pipeline length and improve collection efficiency, the inner diameter of the polyurethane tube should be 1.6mm. Since blood and physiological saline flow through the pipeline, in order to avoid blood sticking to the wall and improve the pipeline cleaning effect, the inner wall surface of the tube should be smooth and hydrophobic.

[0017] The peristaltic pump (8) is connected between the liquid dropper (8) and the waste liquid tank (1-2) through the sixth silicone tube (13-6) and the fifth polyurethane tube (12-5), and is used for cleaning and discharging waste liquid after blood drawing.

[0018] The automatic sampler of the present invention is only applicable to media such as blood, physiological saline and air. Using other media may affect the blood sampling results.

[0019] The automatic sampler described in the present invention is not designed for collecting human blood under any circumstances.

[0020] Beneficial effects of the present invention:

[0021] Drawing and pushing blood through a syringe pump can accurately and quantitatively obtain a set volume of blood, improving the reproducibility and accuracy of blood collection. During the blood drawing process, pure physiological saline is used as the carrier fluid in the syringe pump, and the inside of the pump is free from contamination and corrosion, avoiding cross infection. A semiconductor cold storage room is used to achieve precise temperature control. A peristaltic pump is used to remove residual waste liquid in the pipeline to ensure the cleanliness of the entire pipeline. The entire blood collection process is automated, which not only saves manpower and avoids human errors, but also improves collection efficiency.

[0022] The animals to which the automatic sampler of the present invention is applicable include mice, rats and large animals.

[0023] The automatic sampler of the present invention has high automation and is easy to operate. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The structure diagram of the automatic sampler is shown in FIG. 1 , where: (1-1), (1-2) waste liquid tank; (2-1) first compression valve, (2-2) second compression valve, (2-3) third compression valve, (2-4) fourth compression valve, (2-5) fifth compression valve; (3) mouse; (4) semiconductor cold storage room; (5) collection plate; (6) dropper; (7-1, 7-2) needle; (8) peristaltic pump; (9-1, 9-2) electromagnetic switching valve; (10-1, 10-2) saline bottle; (11-1, 11-2) injection pump; (12-1) first polyurethane tube, (12-2 ) second polyurethane tube, (12-3) third polyurethane tube, (12-4) fourth polyurethane tube, (12-5) fifth polyurethane tube; (13-1) first silicone tube, (13-2) second silicone tube, (13-3) third silicone tube, (13-4) fourth silicone tube, (13-5) fifth silicone tube, (13-6) sixth silicone tube; (14-1, 14-2) blood sensor; (15) four-way connector; (16) three-way connector; (17) reducer; (18) three-way solenoid valve; (19) component control and data collection system. DETAILED DESCRIPTION

[0025] Before blood collection, all valves and pumps are closed. Pour physiological saline into the physiological saline bottles (10-1, 10-2), and the amount of physiological saline in the physiological saline bottles (10-1, 10-2) is not less than 40 mL.

[0026] The first is the stage of cleaning the pipeline before blood collection. The electromagnetic switching valve (9-1) is switched to channel 1, and the injection pump (11-1) draws 1700 μL of physiological saline from the physiological saline bottle (10-1). The electromagnetic switching valve (9-2) is switched to channel 2, the three-way switching valve (18) is switched to channel 3, and the injection pump (11-2) draws 1700 μL of physiological saline from the physiological saline bottle (10-2). The first compression valve (2-1), the third compression valve (2-3), the fifth compression valve (2-5), and the peristaltic pump (8) are opened, the electromagnetic switching valve (9-1) is switched to channel 2, the injection pump (11-1) pushes out 1700 μL of saline solution, the electromagnetic switching valve (9-2) is switched to channel 1, the injection pump (11-2) pushes out 1700 μL of saline solution, the first silicone tube (13-1), the third silicone tube (13-3), the fifth silicone tube (13-5), and the second polyurethane tube (12-2) are cleaned, and the waste liquid enters the waste liquid tank (1-1, 1-2). The first compression valve (2-1), the third compression valve (2-3), the fifth compression valve (2-5), and the peristaltic pump (8) are closed, the electromagnetic switching valve (9-1) is switched to channel 1, and the injection pump (11-1) draws out 1700 μL of saline solution from the saline bottle (10-1). The electromagnetic switching valve (9-2) is switched to channel No. 2, the three-way switching valve (18) is switched to channel No. 3, and the syringe pump (11-2) extracts 1700 μL of physiological saline from the physiological saline bottle (10-2). The first compression valve (2-1), the third compression valve (2-3), the fifth compression valve (2-5), and the peristaltic pump (8) are opened, the electromagnetic switching valve (9-1) is switched to channel No. 2, the syringe pump (11-1) pushes out 700 μL of physiological saline, the electromagnetic switching valve (9-2) is switched to channel No. 1, and the syringe pump (11-2) pushes out 1500 μL of physiological saline, so that the physiological saline fills the four-way connector (15), the second silicone tube (13-2) and the three-way connector (16), and the waste liquid enters the waste liquid tank (1-2). The third compression valve (2-3) and the fifth compression valve (2-5) are closed, the peristaltic pump (8) is closed, the fourth compression valve (2-4) is opened, the electromagnetic switching valve (9-2) is switched to channel 1, the injection pump (11-2) pushes out 200 μL of physiological saline, cleans the fourth silicone tube (13-4), and the waste liquid enters the waste liquid tank (1-1). The first compression valve (2-1) and the fourth compression valve (2-4) are closed, the electromagnetic switching valve (9-2) is switched to channel 2, the three-way switching valve (18) is switched to channel 2, and the injection pump (11-2) extracts 1700 μL of air. The fifth compression valve (2-5) is opened, the peristaltic pump is opened, the electromagnetic switching valve (9-2) is switched to channel 1, the injection pump (11-2) pushes out 1500 μL of air, and the third polyurethane tube (12-3) and the fifth silicone tube (13-5) are emptied.The fifth compression tube valve (2-5) is closed, the peristaltic pump is closed, the three-way switching valve (18) is switched to channel No. 2, the electromagnetic switching valve (9-2) is switched to channel No. 2, and the injection pump (11-2) extracts 1700 μL of air. The first compression tube valve (2-1) and the fourth compression tube valve (2-4) are opened, and the injection pump (11-2) pushes out 1500 μL of air. The first compression tube valve (2-1) and the fourth compression tube valve (2-4) are closed, the three-way electromagnetic valve (18) is switched to channel No. 2, the electromagnetic switching valve (9-2) is switched to channel No. 2, and the injection pump (11-2) extracts 1000 μL of air.

[0027] Then comes the pre-blood collection stage. The mouse (3) is pierced with a needle (7-1). The second compression valve (2-2) and the third compression valve (2-3) are opened, the electromagnetic switching valve (9-1) is switched to channel 2, the injection pump (11-1) pushes 50 μL of saline, and then 20 μL of blood is drawn from the mouse (3), and then the injection pump (11-1) pushes another 50 μL of saline, so as to ensure that the second silicone tube (13-2) will not be blocked during blood collection, thereby affecting the blood collection effect.

[0028] Next comes the blood collection stage. The injection pump (11-1) begins to draw blood from the mouse (3). When the blood enters the four-way connector (15) along the second silicone tube (13-2), the blood sensors (14-1, 14-2) detect the blood, the third compression valve (2-3) is closed, the fourth compression valve (2-4) is opened, the electromagnetic switching valve (9-2) is switched to channel 1, and the injection pump (11-2) draws 200 μL of blood, which is stored in the third polyurethane tube (12-3). The second compression valve (2-2) and the fourth compression valve (2-4) are closed, the first compression valve (2-1) and the third compression valve (2-3) are opened, and the injection pump (11-1) pushes 80 μL of waste liquid into the waste liquid tank (1-1). The first compression valve (2-1) is closed, the second compression valve (2-2) and the fifth compression valve (2-5) are opened, the injection pump (11-1) replenishes physiological saline into the body of the mouse (3), the injection pump (11-2) pushes 500 μL of air, and pushes the blood stored in the third polyurethane tube (12-3) along the fifth silicone tube (13-5), the liquid dropper (6), and the needle (7-2) into the collection plate (5) in the semiconductor cold storage room (4). The peristaltic pump (8) is opened, the injection pump (11-1) continues to replenish physiological saline into the body of the mouse (3), the injection pump (11-2) pushes out the air in the pump, and discharges the waste liquid into the waste liquid tank (1-2). The second compression valve (2-2) is closed, the first compression valve (2-1) is opened, the injection pump (11-1) pushes out the physiological saline in the pump, and discharges the waste liquid into the waste liquid tank (1-1).

[0029] Finally, the blood collection is completed and the pipeline is cleaned. The first compression valve (2-1), the third compression valve (2-3), and the fifth compression valve (2-5) are closed, the peristaltic pump (8) is closed, the electromagnetic switching valve (9-1) is switched to channel 1, and the injection pump (11-1) extracts 1700 μL of physiological saline from the physiological saline bottle (10-1). The electromagnetic switching valve (9-2) is switched to channel 2, the three-way switching valve (18) is switched to channel 3, and the injection pump (11-2) extracts 1700 μL of physiological saline from the physiological saline bottle (10-2). The first compression valve (2-1), the third compression valve (2-3), and the fifth compression valve (2-5) are opened, the peristaltic pump (8) is opened, the electromagnetic switching valve (9-1) is switched to channel 2, the injection pump (11-1) pushes out the physiological saline in the pump, the electromagnetic switching valve (9-2) is switched to channel 1, and the injection pump (11-1) pushes out the physiological saline in the pump. The first compression tube valve (2-1), the third compression tube valve (2-3), and the fifth compression tube valve (2-5) are closed, the peristaltic pump (8) is closed, the electromagnetic switching valve (9-1) is switched to channel 1, and the injection pump (11-1) extracts 1700 μL of physiological saline from the physiological saline bottle (10-1). The electromagnetic switching valve (9-2) is switched to channel 2, the three-way switching valve (18) is switched to channel 3, and the injection pump (11-2) extracts 1700 μL of physiological saline from the physiological saline bottle (10-2). The first compression tube valve (2-1), the third compression tube valve (2-3), and the fifth compression tube valve (2-5) are opened, the peristaltic pump (8) is opened, the electromagnetic switching valve (9-1) is switched to channel No. 2, the injection pump (11-1) pushes 700 μL of physiological saline, and the waste liquid enters the waste liquid tank (1-1), the electromagnetic switching valve (9-2) is switched to channel No. 1, the injection pump (11-2) pushes out 1100 μL of physiological saline, and the waste liquid enters the waste liquid tank (1-2). The third compression tube valve (2-3) and the fifth compression tube valve (2-5) are closed, the fourth compression tube valve (2-4) is opened, the injection pump (11-2) pushes out the physiological saline in the pump, and the waste liquid enters the waste liquid tank (1-1). The first compression tube valve (2-1) and the fourth compression tube valve (2-4) are closed, the peristaltic pump (8) is closed, the three-way switching valve (18) is switched to channel No. 2, the electromagnetic switching valve (9-2) is switched to channel No. 2, and the injection pump (11-2) extracts 1700 μL of air. The first compression tube valve (2-1), the fourth compression tube valve (2-4), and the fifth compression tube valve (2-5) are opened, the peristaltic pump (8) is opened, the electromagnetic switching valve (9-2) is switched to channel No. 1, and the injection pump (11-2) pushes 1300 μL of air. The first compression tube valve (2-1), the fourth compression tube valve (2-4), and the fifth compression tube valve (2-5) are closed, the peristaltic pump (8) is closed, the three-way switching valve (18) is switched to channel No. 2, the electromagnetic switching valve (9-2) is switched to channel No. 2, and the injection pump (11-2) extracts 1700 μL of air.The fourth compression valve (2-4) and the fifth compression valve (2-5) are opened, the electromagnetic switching valve (9-2) is switched to channel 1, and the injection pump (11-2) pushes 1500 μL of air. The fourth compression valve (2-4) and the fifth compression valve (2-5) are closed, the electromagnetic switching valve (9-2) is switched to channel 2, and the injection pump (11-2) extracts 1000 μL of air.

[0030] The above process is a complete blood collection process, and the above process is repeated for multiple blood collections. During blood collection, there is a KVO fluid replenishment process every 30 minutes: the second compression tube valve (2-2) and the third compression tube valve (2-3) are opened, the electromagnetic switching valve (9-1) is switched to channel 2, and the injection pump (11-1) pushes 20μL of saline into the body of the mouse (3). When the saline in the injection pump (11-1) is less than 500μL, the electromagnetic switching valve (9-1) is switched to channel 1, and the injection pump (11-1) extracts 1000μL of saline from the saline bottle (10-1). The purpose of this process is to ensure that the pipeline will not be blocked due to blood coagulation, and to replenish the water lost by the mouse (3) due to physiological activities such as breathing and exercise.

Claims

1. An automatic sampler, characterized in that The invention comprises a waste liquid tank (1-1, 1-2); a first compression pipe valve (2-1), a second compression pipe valve (2-2), a third compression pipe valve (2-3), a fourth compression pipe valve (2-4), and a fifth compression pipe valve (2-5); a semiconductor cold storage room (4); a collection plate (5); a liquid dropper (6); a needle (7-1, 7-2); a peristaltic pump (8); an electromagnetic switching valve (9-1, 9-2); a physiological saline bottle (10-1, 10-2); an injection pump (11-1, 11-2); a first polyurethane tube (12-1), a second polyurethane tube (12- 2), the third polyurethane tube (12-3), the fourth polyurethane tube (12-4), the fifth polyurethane tube (12-5); the first silicone tube (13-1), the second silicone tube (13-2), the third silicone tube (13-3), the fourth silicone tube (13-4), the fifth silicone tube (13-5), the sixth silicone tube (13-6); blood sensors (14-1, 14-2); a four-way connector (15); a three-way connector (16); a reducer (17); a three-way solenoid valve (18); a component control and data collection system (19); The injection pump (11-1, 11-2), the first compression valve (2-1), the second compression valve (2-2), the third compression valve (2-3), the fourth compression valve (2-4), the fifth compression valve (2-5), the peristaltic pump (8) and the blood sensor (14-1, 14-2) are connected via the second polyurethane tube (12-2), the third polyurethane tube (12-3), the first silicone tube (13-1), the second silicone tube (13-2), the third silicone tube (13-3), the fourth silicone tube (13-4), the fifth silicone tube (13-5), the sixth silicone tube (13-6), the four-way connector (15), the three-way connector (1 6) and a reducer (17); the blood sensors (14-1, 14-2) are used to detect blood flowing through the second silicone tube (13-2) and the third silicone tube (13-3); the semiconductor cold storage chamber (4) is used to temporarily store blood; the component control and data collection system (19) controls the operation of the first pressure tube valve (2-1), the second pressure tube valve (2-2), the third pressure tube valve (2-3), the fourth pressure tube valve (2-4), the fifth pressure tube valve (2-5), the peristaltic pump (8), the injection pumps (11-1, 11-2), the electromagnetic switching valves (9-1, 9-2) and the three-way electromagnetic valve (18) to complete blood collection.

2. The automatic sampler according to claim 1, characterized in that The syringe pumps (11-1, 11-2) can extract liquid from the physiological saline bottles (10-1, 10-2) through the electromagnetic switching valves (9-1, 9-2) and the polyurethane tubes (12-1 to 12-4).

3. The automatic sampler according to claim 1, characterized in that The syringe pumps (11-1, 11-2) have a constant flow rate function.

4. The automatic sampler according to claim 1, characterized in that The main material of the semiconductor cold storage room (4) is an aluminum block, and the refrigeration method is semiconductor refrigeration sheet refrigeration.

5. The automatic sampler according to claim 1, characterized in that The blood sensors (14-1, 14-2) are fixed to the four-way connector (15) and measure the change in conductivity of the liquid flowing through the two ends of the pipeline to determine whether blood has been collected.

6. The automatic sampler according to claim 1, characterized in that The peristaltic pump (8) is connected to the fifth polyurethane tube (12-5) and the sixth silicone tube (13-6), and discharges excess waste liquid in the pipeline after blood sampling is completed.

7. The automatic sampler according to claim 1, characterized in that The inner diameter of the polyurethane tube (12-1 to 12-5) is 1.6 mm.

8. The automatic sampler according to claim 1, characterized in that The inner diameter of the silicone tube (13-1 to 13-6) is 0.4 mm.

9. The automatic sampler according to claim 1, characterized in that The first compression pipe valve (2-1) is connected between the waste liquid tank (1-1) and the four-way joint (15) through a first silicone tube (13-1); the second compression pipe valve (2-2) is connected between the needle head (7-1) and the four-way joint (15) through a second silicone tube (13-2); the third compression pipe valve (2-3) is connected between the three-way switching valve and the four-way joint (15) through a third silicone tube (13-3), a reducer (17), and a second polyurethane tube (12-2); the fourth compression pipe valve (2-4) is connected between the four-way joint (15) and the three-way joint (16) through a fourth silicone tube (13-4); and the fifth compression pipe valve (2-5) is connected between the three-way joint (16) and the liquid dropper (6) through a fifth silicone tube (13-5).

10. The automatic sampler according to claim 1, characterized in that The injection pumps (11-1, 11-2) extract physiological saline through the electromagnetic switching valves (9-1, 9-2) and the three-way electromagnetic valve (18), and clean the pipeline; after the cleaning is completed, the injection pump (11-1) starts to extract blood, and when the blood sensors (14-1, 14-2) detect blood, the injection pump (11-2) starts to extract a set volume of blood; after the set volume of blood is extracted, the injection pump (11-2) pushes the blood into the semiconductor cold storage chamber (4).

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