Incubation box, temperature control shaking table and incubation work station

Abstract

The utility model discloses an incubation box, a temperature control shaking table and an incubation workstation. The incubation box comprises a first box body, the second box body is used for covering the first box body, and a containing cavity is defined by the first box body and the second box body; the first temperature control mechanism is used for regulating and controlling the temperature of the incubation box and comprises a first temperature sensor used for measuring the temperature of the incubation box and a second temperature sensor used for measuring the temperature of the incubation box; the upper surface of the refrigeration assembly is attached to the lower surface of the first box body, and the refrigeration assembly is used for reducing the temperature of the incubation box. The incubation box is provided with the independent temperature control assembly, and the temperature of the incubation box in the incubation process can be controlled.

Description

Technical Field

[0001] The present application relates to an experimental instrument, and in particular to an incubation box, a temperature-controlled shaker and an incubation workstation. Background Art

[0002] The incubation workstation is a device that automates the blocking, incubation and washing processes in Western Blot protein immunoblotting experiments. In Western Blot experiments, after transferring the protein from the gel to a solid support such as a nitrocellulose membrane or a polyvinylidene fluoride membrane, an antibody incubation process is required. The entire manual incubation process takes a long time, is cumbersome to operate, and has low technical content, and urgently needs to be automated. Utility Model Content

[0003] Purpose of the utility model: In order to solve the problems of the prior art, the utility model provides an incubation box, a temperature-controlled shaker and an incubation workstation. The incubation box of the present application is provided with an independent temperature control component, which can control the temperature of the incubation box during the incubation process.

[0004] Technical solution: An embodiment of the present application provides an incubation box, comprising a first box body; a second box body, the second box body is used to cover the first box body, the first box body and the second box body form a accommodating cavity; a first temperature control mechanism, the first temperature control mechanism is used to adjust the temperature of the incubation box, including: a first temperature sensor, the first temperature sensor is used to measure the temperature of the incubation box; a refrigeration assembly, the upper surface of the refrigeration assembly is in contact with the lower surface of the first box body, and is used to reduce the temperature of the incubation box.

[0005] In some embodiments, the first temperature control mechanism further includes a heat insulation component, and the heat insulation component is disposed below the refrigeration component.

[0006] In some embodiments, the first temperature control mechanism further includes a heat dissipation component, and the heat dissipation component is connected to the refrigeration component.

[0007] In some embodiments, the upper surface of the first box body is recessed downwards, and forms the accommodating cavity together with the lower surface of the second box body.

[0008] An embodiment of the present application also provides a temperature-controlled shaker, comprising: a box body assembly, which is used to place an incubation box; a second temperature control mechanism, which is used to adjust the temperature of the box body assembly; and a first driving mechanism, which is connected to the box body assembly and is used to drive the box body assembly to shake.

[0009] In some embodiments, the first driving mechanism includes a first driving motor and a connecting rod mechanism connected to an output end of the first driving motor.

[0010] In some embodiments, a first connecting shaft and a second connecting shaft are disposed below the box body assembly, and the first connecting shaft is connected to the connecting rod mechanism.

[0011] An embodiment of the present application also provides an incubation workstation, comprising: a pipetting component, which is used to absorb liquid; a reagent compartment, which is used to store reagents required by the pipetting component; a heat dissipation component, which is used to dissipate heat in the incubation workstation; and a temperature-controlled shaker, which is used to provide an incubation environment.

[0012] In some embodiments, the pipetting assembly includes a needle assembly and a second drive mechanism for controlling the position of the needle assembly.

[0013] In some embodiments, a plurality of through holes are provided above the reagent chamber, and the pipetting assembly pipettes the solution through the through holes.

[0014] Beneficial effects: Compared with the prior art, the present application provides an incubation box, a temperature-controlled shaker, and an incubation workstation. The incubation box of the present application includes a first box body; a second box body, the second box body is used to cover the first box body, the first box body and the second box body form a accommodating cavity; a first temperature control mechanism, the first temperature control mechanism is used to adjust the temperature of the incubation box, including: a first temperature sensor, the first temperature sensor is used to measure the temperature of the incubation box; a refrigeration component, the upper surface of the refrigeration component is in contact with the lower surface of the first box body, and is used to reduce the temperature of the incubation box. The incubation box of the present application is provided with an independent temperature control component, which can control the temperature of the incubation box during the incubation process. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of the structure of the incubation box in the embodiment of the present application;

[0016] Figure 2 This is a schematic diagram of the structure of the incubation box in the embodiment of the present application;

[0017] Figure 3 for Figure 1 Cross-sectional view of the AA surface of the middle incubation box;

[0018] Figure 4 This is a schematic diagram of the structure of a temperature-controlled shaking table in an embodiment of the present application;

[0019] Figure 5 This is a schematic diagram of the structure of a temperature-controlled shaking table in an embodiment of the present application;

[0020] Figure 6 This is a schematic diagram of the structure of the incubation workstation in the embodiment of the present application;

[0021] Figure markings: 1-incubation box, 100-accommodating chamber, 110-first box body, 120-second box body, 130-first temperature control mechanism, 131-first temperature sensor, 132-refrigeration component, 133-thermal insulation component, 134-heat dissipation component, 2-temperature-controlled shaker, 210-box body component, 211-first connecting shaft, 212-second connecting shaft, 220-second temperature control mechanism, 230-first driving mechanism, 231-first driving motor, 232-connecting rod mechanism, 3-incubation workstation, 310-pipetting component, 311-needle assembly, 312-second driving mechanism, 313-pump assembly, 320 reagent chamber, 321-through hole, 330-heat dissipation component, 331-water pump, 332-water-cooled radiator, 340-circuit control component. DETAILED DESCRIPTION

[0022] The solution of the present application is further described below in conjunction with the accompanying drawings.

[0023] The process of Western Blot protein immunoblotting experiment includes: placing the membrane with transferred protein into the blocking solution and performing the blocking process at 37°C for about 1 hour. This process is to block the redundant sites on the membrane to avoid affecting the subsequent steps; after the blocking is completed, a washing operation is required. The liquid in the incubation box is replaced with a washing solution, and the washing is performed on a shaker at 37°C for 3 times, each time for 5 to 10 minutes. After each washing, the washing solution is replaced with a new washing solution. The time and number of times used in different laboratories may vary. This process is to remove the residual blocking solution on the membrane; after washing, the primary antibody incubation operation is performed. Replace the washing solution with the primary antibody diluent, incubate at 37°C for 1 hour, or incubate at 4°C overnight, bind the primary antibody to the target protein, and prepare for the subsequent steps. The primary antibody diluent can be recycled and reused; perform the washing operation again, and the process is the same as the first washing; after washing, add the secondary antibody diluent to the incubation box and incubate at 37°C for 1 hour. This step is to bind the secondary antibody to the primary antibody that has been bound to the target protein. The secondary antibody carries a substance that can make the chemiluminescent reagent glow, and the light signal can be detected by the imaging device; after the secondary antibody incubation is completed, the final washing operation needs to be performed, and the process is the same as the previous washing operation. From the above steps, it can be seen that the incubation process of Western Blot protein immunoblotting experiment is cumbersome, the operation time is long, and it consumes a lot of time and labor; in addition, it does not have a multi-channel independent incubation process, which is not conducive to the comparison of experimental results; the primary antibody incubation process needs to be controlled at around 4°C, and the primary antibody reagent used in the experiment also needs to be stored at 4°C. During the operation, the temperature of the reagent cannot be accurately controlled, and the temperature cannot be accurately controlled during the incubation process. In order to solve at least one of the above problems, such as Figure 1 , Figure 2 and Figure 3As shown, the embodiment of the present application provides an incubation box 1, comprising a first box body 110, a second box body 120, and a first temperature control mechanism 130; the second box body 120 of the present application is used to cover the first box body 110, and the first box body 110 and the second box body 120 form a receiving cavity 100; in some specific embodiments, the upper surface of the first box body 110 is concave downward to form an arc surface, and the lower surface of the second box body 120 forms a receiving cavity 100. The concave arc cavity formed by the first box body 110 facilitates the outflow of the solution and the recovery of the solution.

[0024] The first temperature control mechanism 130 of the present application is used to regulate the temperature of the incubation box 1. In some specific applications, the first temperature control mechanism 130 includes a first temperature sensor 131, which is used to measure the temperature of the incubation box. The first temperature control mechanism 130 of the present application also includes a refrigeration component 132. In a specific application example, the refrigeration component 132 can select a TEC refrigeration module. The upper surface of the refrigeration component 132 is attached to the lower surface of the first box body 110 to reduce the temperature of the incubation box. In some embodiments, the first temperature control mechanism 130 also includes: an insulation component 133, which is arranged below the refrigeration component 132. The insulation component 133 is used to further isolate the heat exchange of the incubation box and improve the refrigeration effect of the refrigeration component 132 on the incubation box. In some embodiments, the first temperature control mechanism 130 also includes: a heat dissipation component 134, which is connected to the refrigeration component 132. In some specific application examples, the heat dissipation component 134 is a water-cooled heat sink for heat dissipation of the refrigeration component 132. The incubation box of the present application is provided with an independent refrigeration module, and the temperature of the independent module can accurately control the temperature of the reagents in the experiment, thereby improving the reliability of the experiment and reducing the cost of the experiment.

[0025] like Figure 4 and Figure 5 As shown, the embodiment of the present application provides a temperature-controlled shaking table 2, including: a box assembly 210, the box assembly 210 is used to place the incubation box; a second temperature control mechanism 220, the second temperature control mechanism 220 is used to adjust the temperature of the box assembly 210; a first drive mechanism 230, the first drive mechanism 230 is connected to the box assembly 210, and is used to drive the box assembly 210 to shake. The box assembly 210 of the present application is used to provide a space for placing the incubation box 1, and the second temperature control mechanism 220 is used to control the temperature in the box assembly 210. In some specific embodiments, the second temperature control mechanism 220 includes a heating component and a temperature sensor, which are used to control the temperature in the box assembly 210. The second temperature control mechanism 220 of the present application can also adopt any temperature control component in the prior art that can achieve the purpose of the present application.

[0026] In some embodiments, the first driving mechanism 230 includes a first driving motor 231, a connecting rod mechanism 232 connected to the output end of the first driving motor 231, and the connecting mechanism 232 is used to connect the output end of the first driving motor 231 and the box body assembly 210 to achieve the shaking of the box body assembly 210 during the incubation process. In some embodiments, a first connecting shaft 211 and a second connecting shaft 212 are arranged below the box body assembly 210, and the first connecting shaft 211 is connected to the connecting rod mechanism 232. In order to improve the stability of the box body assembly 210 during the shaking process, the present application is provided with two second connecting shafts 212 on both sides of the bottom surface of the box body assembly 210, and the second connecting shaft 212 is a rocking table bearing in a specific application, and the box body assembly 210 rotates around the second connecting shaft 212.

[0027] like Figure 6 As shown, the embodiment of the present application provides an incubation workstation, including: a pipetting component 310, the pipetting component 310 is used for absorbing liquid; a reagent compartment 320, the reagent compartment 320 is used to store the reagents required by the pipetting component 310; a heat dissipation component 330, the heat dissipation component 330 is used for heat dissipation of the incubation workstation; a temperature-controlled shaker 2, the temperature-controlled shaker 2 is used to provide an incubation environment. The incubation workstation of the present application replaces manual labor and can automatically realize complex incubation experiments. The temperature-controlled shaker 2 and the incubation box 1 in the incubation workstation can accurately control the temperature in the experiment through an independent temperature control module, thereby improving the reliability of the experiment.

[0028] In some embodiments, the liquid transfer assembly 310 includes a needle assembly 311 and a second drive mechanism 312 for controlling the position of the needle assembly 311. The needle assembly 311 includes multiple needles to achieve multi-channel liquid extraction, which greatly improves the experimental efficiency. The needle assembly 311 of the present application controls the liquid transfer volume of the needle assembly 311 through a pump assembly 313. In some specific embodiments, the pump assembly 313 includes a peristaltic pump corresponding to the number of needles. The peristaltic pump is pre-placed in a reagent storage area integrated with a temperature control module, and a heat control assembly 330 and a temperature control shaker 2 are integrated in the incubation area. Only by placing the membrane in the incubation box 1, the reagent can be automatically added to the incubation box, and the reagent can be automatically discharged and recovered after a process is completed. Through high-degree-of-freedom programming operations, the customary operation requirements of various laboratories, different proteins, and different operators are met, eliminating the cumbersome and time-consuming traditional manual incubation operation process. In some specific embodiments, the second driving mechanism 312 can realize the up and down movement and left and right movement of the needle assembly 311, which can be realized specifically by driving the motor and the sliding assembly including a lead screw and a slide rail. In some embodiments, a plurality of through holes 321 are provided above the reagent compartment 320, and the pipetting assembly 310 pipettes the solution through the through holes 321. In some embodiments, the heat dissipation assembly 330 of the present application is a water-cooled heat dissipation assembly, including a water pump 331 and a water-cooled radiator 332, and the water-cooled radiator 332 can select a fin-type water-cooled radiator. In order to realize the electrical control of the incubation workstation 3 of the present application, the present application also provides a circuit control assembly 340, and the circuit control assembly 340 is used to realize the electrical connection and system control of the incubation workstation.

[0029] Working principle: put the configured primary antibody, secondary antibody, washing and blocking solution into the reagent compartment 320. The reagent compartment 320 has a 4°C refrigeration function through the set temperature control module. Put the experimental incubation mold into the incubation box 1, add the experimental process, set the temperature, and the instrument automatically performs the incubation process. Four different primary antibody incubations can be performed at the same time. The left and right and up and down position control of the needle assembly 311 is realized through the two motor assemblies in the second drive mechanism 312 to absorb and add liquid to different reagents. At the same time, the temperature-controlled shaker 2 swings up and down to fully combine and wash clean. When recovering the liquid, the temperature-controlled shaker will tilt to increase the amount of reagent recovery.

[0030] The incubation workstation of the present application can automatically complete the reagent addition, discharge, and recovery operations during the sealing, incubation, and washing processes, and integrates a shaker and temperature control module to minimize manpower requirements. After the process is completed, the color development and exposure operation can be carried out directly.

Claims

1. An incubation box, characterized in that: It comprises a first box body (110); a second box body (120), the second box body (120) being used to cover the first box body (110), the first box body (110) and the second box body (120) forming a containing cavity (100); A first temperature control mechanism (130), the first temperature control mechanism (130) is used to control the temperature of the incubation box, comprising: A first temperature sensor (131), the first temperature sensor (131) is used to measure the temperature of the incubation box; A refrigeration component (132), the upper surface of which is in contact with the lower surface of the first box body (110), and is used to reduce the temperature of the incubation box.

2. The incubation box according to claim 1, characterized in that: The first temperature control mechanism (130) further comprises a heat insulation component (133), wherein the heat insulation component (133) is arranged below the refrigeration component (132).

3. The incubation box according to claim 1, characterized in that: The first temperature control mechanism (130) further comprises a heat dissipation component (134), wherein the heat dissipation component (134) is connected to the refrigeration component (132).

4. The incubation box according to claim 1, characterized in that: The upper surface of the first box body (110) is concave downwards, and together with the lower surface of the second box body (120) forms the accommodating cavity (100).

5. A temperature-controlled shaking table, characterized in that: include: A box body assembly (210), the box body assembly (210) being used to place the incubation box according to claim 1; A second temperature control mechanism (220), the second temperature control mechanism (220) being used to control the temperature of the box assembly (210); A first driving mechanism (230), the first driving mechanism (230) is connected to the box body assembly (210) and is used to drive the box body assembly (210) to shake.

6. The temperature-controlled shaking table according to claim 5, characterized in that: The first driving mechanism (230) comprises a first driving motor (231) and a connecting rod mechanism (232) connected to an output end of the first driving motor (231).

7. The temperature-controlled shaking table according to claim 6, characterized in that: A first connecting shaft (211) and a second connecting shaft (212) are provided below the box body assembly (210), and the first connecting shaft (211) is connected to the connecting rod mechanism (232).

8. An incubation workstation, characterized in that: include: A liquid transfer assembly (310), wherein the liquid transfer assembly (310) is used for aspirating liquid; A reagent chamber (320), the reagent chamber (320) being used to store reagents required by the liquid transfer assembly (310); A heat dissipation component (330), the heat dissipation component (330) being used for dissipating heat of the incubation workstation; The temperature-controlled shaker (2) according to claim 5, wherein the temperature-controlled shaker (2) is used to provide an incubation environment.

9. The incubation workstation according to claim 8, characterized in that: The liquid transfer assembly (310) comprises a needle assembly (311) and a second driving mechanism (312) for controlling the position of the needle assembly (311).

10. The incubation workstation according to claim 8, characterized in that: A plurality of through holes (321) are provided above the reagent chamber (320), and the liquid transfer component (310) transfers the solution through the through holes (321).

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