Flow injection analysis device

Abstract

The utility model provides a flow injection analysis device, which comprises a transfusion unit, a mixed reaction unit, a detector and a data processing system which are connected in sequence, the mixed reaction unit comprises a four-channel switching valve, the four-channel switching valve is switched between a first working state and a second working state, and the detector is connected with the data processing system. The four-channel switching valve comprises a first switching port, a second switching port, a third switching port and a fourth switching port, the first switching port is communicated with the infusion unit, the second switching port is communicated with one end of the stirrer, the other end of the stirrer is communicated with the third switching port, and the fourth switching port is communicated with the third switching port. The fourth switching port is communicated with one end of a reactor, the other end of the reactor is communicated with a detector, and an ultrasonic generator is arranged on the outer side of the reactor. The flow direction of liquid can be changed, the liquid flows through the stirrer for multiple times to be stirred, mixing of a sample and a reagent is promoted, and meanwhile the ultrasonic generator is adopted to further promote mixing of the sample and the reagent.

Description

Technical Field

[0001] The utility model relates to the technical field of chemical analysis, in particular to a flow injection analysis device. Background Art

[0002] Flow injection analysis is a continuous flow analysis technology that reproduces the quantitative injection and rapid mixing reaction of samples and reagents in a liquid flow. This technology injects a certain volume of sample solution into a flowing, non-air-gapped continuous carrier of reagent solution. The injected sample solution flows into the reaction coil to form an area, mixes and reacts with the reagent in the carrier, and then enters the flow detector for measurement, analysis and recording. It is widely used in the fields of biomedicine, food analysis, metallurgical analysis and environmental analysis. When dealing with complex samples or requiring high-sensitivity analysis, the existing flow injection analysis often has the problem of insufficient mixing between samples and reaction reagents, which leads to low reaction efficiency between samples and reaction reagents and large errors in the detection results of the detector. Utility Model Content

[0003] The utility model aims to solve the shortcomings in the prior art and proposes a flow injection analysis device.

[0004] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0005] A flow injection analysis device comprises an infusion unit, a mixing reaction unit, a detector and a data processing system connected in sequence;

[0006] The mixing reaction unit comprises a four-channel switching valve, a stirrer, a reactor and an ultrasonic generator, wherein a first switching port of the four-channel switching valve is communicated with the infusion unit, a second switching port of the four-channel switching valve is communicated with one end of the stirrer, the other end of the stirrer is communicated with a third switching port of the four-channel switching valve, a fourth switching port of the four-channel switching valve is communicated with one end of the reactor, the other end of the reactor is communicated with the detector, and the ultrasonic generator is arranged on the outside of the reactor;

[0007] The four-channel switching valve switches between the first working state and the second working state:

[0008] The first working state: the first switch port is connected to the second switch port, and the third switch port is connected to the fourth switch port;

[0009] The second working state: the first switch port is connected to the third switch port, and the second switch port is connected to the fourth switch port.

[0010] Furthermore, the infusion unit includes a sample channel, a reagent channel and an injection pump, and the sample channel and the reagent channel are connected to the mixing reaction unit through the injection pump.

[0011] Furthermore, the injection pump is a multi-channel pump.

[0012] Furthermore, the agitator includes a shell, one end of the shell is provided with a liquid inlet, the other end of the shell is provided with a liquid outlet, the liquid inlet is connected to the second switching port of the four-channel switching valve, the liquid outlet is connected to the third switching port of the four-channel switching valve, one end of the shell is provided with a drive motor, the inner cavity of the shell is provided with a stirring paddle, and the output end of the drive motor is connected to the stirring paddle.

[0013] Compared with the prior art, the utility model has the following technical effects:

[0014] In the utility model, the four-channel switching valve can change the flow direction of the mixed liquid of the sample and the reagent in the flow injection analysis device by switching between the first working state and the second working state, so that the liquid flows through the agitator for multiple times, and the agitator can stir the liquid, so that the sample and the reagent can obtain a better mixing effect. An ultrasonic generator is arranged outside the reactor, and when the liquid enters the reactor, the ultrasonic generator generates ultrasonic vibration to further mix and react the sample and the reagent more fully. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 A schematic diagram of a flow injection analysis device according to an embodiment of the present invention;

[0016] Figure 2 It is a schematic diagram of a four-channel switching valve in a first working state according to an embodiment of the utility model;

[0017] Figure 3 It is a schematic diagram of the four-channel switching valve in the second working state according to an embodiment of the utility model;

[0018] Figure 4 The figure is a schematic structural diagram of an agitator according to an embodiment of the utility model.

[0019] Explanation of the reference numerals: 1. Infusion unit, 11. Sample channel, 12. Reagent channel, 13. Sampling pump, 2. Mixing reaction unit, 21. Four-channel switching valve, 211. First switching port, 212. Second switching port, 213. Third switching port, 214. Fourth switching port, 22. Agitator, 221. Shell, 222. Liquid inlet, 223. Liquid outlet, 224. Driving motor, 225. Stirring paddle, 23. Reactor, 24. Ultrasonic generator, 3. Detector, 4. Data processing system. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0021] As an example, see Figure 1 This embodiment provides a flow injection analysis device, including an infusion unit 1, a mixing reaction unit 2, a detector 3 and a data processing system 4 connected in sequence. The detector 3 can use a light-emitting diode, a fluorescence detector or other detection device suitable for different reaction products, and can detect the reaction products in real time. The data processing system 4 can use a computer, and the computer receives, processes and analyzes the detection signal obtained by the detector 3 to obtain the final analysis result.

[0022] Among them, the mixing reaction unit 2 includes a four-channel switching valve 21, an agitator 22, a reactor 23 and an ultrasonic generator 24. The four-channel switching valve 21 has four switching ports, including a first switching port 211, a second switching port 212, a third switching port 213 and a fourth switching port 214. The first switching port 211 of the four-channel switching valve 21 is connected to the infusion unit 1, the second switching port 212 of the four-channel switching valve 21 is connected to one end of the agitator 22, the other end of the agitator 22 is connected to the third switching port 213 of the four-channel switching valve 21, the fourth switching port 214 of the four-channel switching valve 21 is connected to one end of the reactor 23, and the other end of the reactor 23 is connected to the detector 3. The end of the detector 3 away from the reactor 23 is electrically connected to the data processing system 4. The ultrasonic generator 24 is installed on the outside of the reactor 23, and the ultrasonic generator 24 is used to generate ultrasonic vibrations to promote mixing of reagents and samples.

[0023] See also Figures 2 to 3 The four-channel switching valve 21 has two working states, including a first working state and a second working state. The four-channel switching valve 21 can switch between the first working state and the second working state:

[0024] First working state: the first switch port 211 is connected to the second switch port 212, and the third switch port 213 is connected to the fourth switch port 214;

[0025] Second working state: the first switch port 211 is connected to the third switch port 213 , and the second switch port 212 is connected to the fourth switch port 214 .

[0026] The four-channel switching valve 21 includes a stator and a rotor. Different states can be switched by rotating the rotor, and the rotor can be driven by a motor.

[0027] The four-channel switching valve 21 can change the flow direction of the sample and reagent mixed liquid in the mixed reaction unit 2 by switching between the first working state and the second working state. After the reversal, the liquid can flow through the stirrer 22 for multiple times. The stirrer 22 stirs the liquid so that the sample and the reagent can be more fully mixed and reacted, reducing the detection error of the detector 3 and improving the sensitivity and accuracy of the entire device. An ultrasonic generator 24 is also installed on the outside of the reactor 23. The liquid stirred multiple times by the stirrer 22 enters the reactor 23. The ultrasonic generator 24 can generate ultrasonic vibration to further promote the mixing and reaction of the sample and the reagent, and further improve the sensitivity and accuracy of the detection.

[0028] The reactor 23 can be designed in a spiral shape or in other shapes. The reactor 23 is also provided with a temperature control mechanism or other equipment for providing suitable reaction conditions to promote the reaction between the reagent and the sample.

[0029] The infusion unit 1 includes a sample channel 11, a reagent channel 12 and an injection pump 13, and the sample channel 11 and the reagent channel 12 are connected to the mixed reaction unit 2 through the injection pump 13. The sample channel 11 is used to inject the sample to be tested into the mixed reaction unit 2, and the reagent channel 12 is used to continuously inject the reagent solution into the mixed reaction unit 2, thereby realizing independent injection between the sample and the reagent. The end of the sample channel 11 is connected to the end of the reagent channel 12, and the reagent solution flows continuously in the entire device. The sample is preliminarily mixed with the reagent solution through the sample channel 11 and then enters the mixed reaction unit 2. The injection pump 13 is a multi-channel pump that can transport a variety of different liquids at the same time, and the liquids are transported independently between multiple channels of the multi-channel pump.

[0030] See also Figure 4 The stirrer 22 includes a housing 221, a liquid inlet 222 is provided at one end of the housing 221, a liquid outlet 223 is provided at the other end of the housing 221, the liquid inlet 222 is communicated with the second switching port 212 of the four-channel switching valve 21, and the liquid outlet 223 is communicated with the third switching port 213 of the four-channel switching valve 21, a driving motor 224 is provided at one end of the housing 221, a stirring paddle 225 is provided in the inner cavity of the housing 221, and the output end of the driving motor 224 is connected to the stirring paddle 225. When working, the driving motor 224 drives the stirring paddle 225 to rotate, the liquid flows into the inner cavity of the housing 221 from the liquid inlet 222, and flows out of the inner cavity of the housing 221 from the liquid outlet 223, and the rotating stirring paddle 225 stirs the flowing liquid to promote the mixing of the sample and the reagent.

[0031] The flow injection analysis device described in this embodiment continuously injects the reagent solution into the mixing reaction unit 2 through the infusion unit 1. The reagent solution flows continuously in the entire device. This part of the liquid is called "carrier current". The sample is injected into the mixing reaction unit 2 through the infusion unit 1 at a certain regular time interval. After the quantitative sample is injected into the mixing reaction unit 2 for the first time, the multi-channel pump closes the channel for transporting the sample, and the sample is no longer injected. At this time, the four-channel switching valve 21 of the mixing reaction unit 2 is in the first working state, and the reagent and sample enter the stirrer 22 in a positive direction. The stirrer 22 stirs the entering reagent and sample. When the reagent and sample flow out of the stirrer 22 and do not reach the third switching port 213, the four-channel switching valve 21 is switched to the second working state, and the movement direction of the reagent and sample is changed. Next, the reagent and sample enter the agitator 22 in the reverse direction, and the agitator 22 stirs the reagent and sample entering again. When the reagent and sample flow out of the agitator 22 and do not reach the second switching port 212, the four-channel switching valve 21 is switched to the first working state, and the movement direction of the reagent and sample changes again. The reagent and sample flow through the agitator 22 again. After multiple reversals and stirring by the agitator 22, the reagent and sample are fully mixed. The reagent and sample stirred multiple times by the agitator 22 enter the reactor 23. The ultrasonic generator 24 can generate ultrasonic vibrations to further promote the mixing and reaction of the sample and the reagent. The reaction product is transported to the detector 3 for detection. The data processing system 4 receives, processes and analyzes the detection signal obtained by the detector 3 to obtain the analysis result, and then performs a second sample detection and analysis.

[0032] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

Claims

1. A flow injection analysis device, characterized in that: It comprises an infusion unit (1), a mixing reaction unit (2), a detector (3) and a data processing system (4) which are connected in sequence; The mixing reaction unit (2) comprises a four-channel switching valve (21), a stirrer (22), a reactor (23) and an ultrasonic generator (24); a first switching port (211) of the four-channel switching valve (21) is in communication with the infusion unit (1); a second switching port (212) of the four-channel switching valve (21) is in communication with one end of the stirrer (22); the other end of the stirrer (22) is in communication with a third switching port (213) of the four-channel switching valve (21); a fourth switching port (214) of the four-channel switching valve (21) is in communication with one end of the reactor (23); the other end of the reactor (23) is in communication with the detector (3); and the ultrasonic generator (24) is arranged outside the reactor (23); The four-channel switching valve (21) switches between a first working state and a second working state: The first working state: the first switch port (211) is connected to the second switch port (212), and the third switch port (213) is connected to the fourth switch port (214); The second working state: the first switch port (211) is connected to the third switch port (213), and the second switch port (212) is connected to the fourth switch port (214).

2. The flow injection analysis device according to claim 1, characterized in that: The infusion unit (1) comprises a sample channel (11), a reagent channel (12) and an injection pump (13); the sample channel (11) and the reagent channel (12) are connected to the mixing reaction unit (2) via the injection pump (13).

3. The flow injection analysis device according to claim 2, characterized in that: The sample injection pump (13) is a multi-channel pump.

4. The flow injection analysis device according to claim 1, characterized in that: The stirrer (22) comprises a shell (221), one end of the shell (221) is provided with a liquid inlet (222), the other end of the shell (221) is provided with a liquid outlet (223), the liquid inlet (222) is communicated with the second switch port (212) of the four-channel switch valve (21), the liquid outlet (223) is communicated with the third switch port (213) of the four-channel switch valve (21), one end of the shell (221) is provided with a drive motor (224), the inner cavity of the shell (221) is provided with a stirring paddle (225), and the output end of the drive motor (224) is connected to the stirring paddle (225).

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