Blood collection device for SAKI model construction

Abstract

The utility model provides a blood collection device for SAKI model construction, and belongs to the technical field of blood sampling devices. The infusion apparatus comprises an injector and an infusion needle, the injector comprises a barrel, a plug rod and a piston, the plug rod is provided with four wing plates to form a cross-shaped structure, and a containing cavity is formed between every two adjacent wing plates; the infusion needle comprises a needle head and a pipeline; one end of the pipeline is connected with the needle, and the other end is connected with the nipple of the barrel; the clamping and closing device is detachably arranged on the pipeline and can realize opening and closing of the pipeline; the at least one positioning groove is formed in the at least one wing plate; the locking piece is detachably connected to the cylinder body; the locking piece can be connected with the positioning groove in a matched and clamped mode so that the plug rod can be positioned. The clip-off device is used in cooperation with the locking piece, and after the plug rod is pulled, the syringe can form and keep a negative pressure state. When the device is used for collecting blood samples, the blood collection amount is large, the blood flow is large, pollution is avoided, the blood quality can be guaranteed to the maximum extent, and operation is easy.

Description

Technical Field

[0001] The utility model relates to the technical field of blood sampling devices, in particular to a blood sampling device for constructing a SAKI model. Background Art

[0002] Septic shock is a systemic inflammatory response syndrome caused by severe infection, accompanied by persistent hypotension that cannot be corrected even after adequate fluid resuscitation. Septic acute kidney injury (SAKI) is one of the common complications of septic shock, which can lead to a sharp decline in renal function, oliguria or anuria, water and electrolyte imbalance, metabolic acidosis and other symptoms.

[0003] In the task of "Study on the role and mechanism of AKR1B1 in the progression of septic acute kidney injury", four time points were set at 0h, 6h, 12h, and 24h after the mice were modeled. Blood was collected at each time point to detect serum creatinine (12r) and serum urea nitrogen (BUN), urine was collected to detect urine NGAL and KIM-1 levels, and mouse serum and kidney tissue were taken for relevant experimental analysis.

[0004] The above experiments require the use of a blood collection device. Traditional methods for collecting blood from mice include tail cutting, eye removal, heart blood collection, and decapitation blood collection. The many existing blood collection methods each have their own advantages and disadvantages: for example, the eye removal blood collection method cannot be repeated, and can cause blindness in mice, is prone to infection, and can cause death of mice when bleeding is not strictly stopped. It also does not comply with the basic principles of animal welfare; heart blood collection can obtain more blood, but this method can only be used once, and the animal will die, and blood cannot be drawn continuously; in addition, the operating requirements are also high; although the tail cutting method can draw blood multiple times in a row, the amount of blood collected each time is extremely small, which is not suitable for experiments requiring a large amount of blood.

[0005] Compared with the above traditional blood collection methods, there is a new blood collection method - submandibular venous plexus blood collection method, which is not only less harmful to animals, but also can be used for multiple live blood collections. Compared with traditional blood collection methods, submandibular venous plexus blood collection method is more in line with the 3R principle and experimental animal welfare and ethical standards, and also meets the blood collection needs of this experiment.

[0006] At present, the main operation method of the submandibular venous plexus blood collection method is: accurately grasp the position of the submandibular venous plexus blood vessels in the mandibular part of the mouse head. The blood vessels are located at the edge of the masseter muscle behind the mandible. After wiping the blood collection site with an alcohol cotton ball for disinfection, quickly insert the sterile injection needle into the submandibular venous plexus blood vessels, and the blood will flow out. The blood is collected in a sterile centrifuge tube. However, the main problems with this method are: (1) Since the blood flows out from the mandibular hair, it may adhere to the hair and contaminate the blood, affecting the experimental results. (2) The blood flow is small and the amount of blood collected is small, which is not easy to meet the experimental needs. Utility Model Content

[0007] The utility model aims to solve one of the technical problems in the related art at least to a certain extent. To this end, the utility model proposes a blood collection device for constructing a SAKI model, which can ensure the quality of blood to the greatest extent and is easy to operate.

[0008] The technical solution of the utility model to solve the technical problem is:

[0009] The technical solution proposes a blood collection device for constructing a SAKI model, including a syringe and an infusion needle. The syringe includes a barrel, a plug rod and a piston. The plug rod has four wing plates to form a cross-shaped structure, and a receiving cavity is formed between adjacent wing plates. The infusion needle includes a needle and a pipeline. One end of the pipeline is connected to the needle, and the other end is connected to the nipple of the barrel. It also includes:

[0010] A clamping device, which is detachably arranged on the pipeline and can realize the opening and closing of the pipeline;

[0011] A positioning groove, at least one of which is provided, is provided on at least one of the wing plates;

[0012] A locking member is detachably connected to the cylinder body; the locking member can be adapted to engage with the positioning groove to achieve positioning of the plug rod;

[0013] The clamp is used in conjunction with the locking element to enable the syringe to form and maintain a negative pressure state after the plug rod is pulled out.

[0014] Preferably, the locking member includes a clamping plate, the tail end of the cylinder has a flange, one end of the clamping plate is connected to the flange surface, and the other end extends into the accommodating cavity; the plug rod and the piston can rotate relative to each other; when the plug rod is rotated, the positioning groove can engage with the clamping plate to axially lock and position the plug rod.

[0015] Preferably, the flange is provided with a positioning hole, the clamping plate is connected with a threaded column, and one end of the threaded column passes through the positioning hole and is threadedly connected with a nut.

[0016] Preferably, the flange is provided with a positioning hole, the clamping plate is connected with a clamping hook, and one end of the clamping hook passes through the positioning hole and is clamped with an end cap.

[0017] Preferably, the locking member comprises a clip, which is detachably clamped on the cylinder, and the clip is provided with a column, and a locking tongue is connected to the column, and the locking tongue can be adapted to be clamped with the positioning groove.

[0018] Preferably, the column is provided with a through hole, one end of the lock tongue passes through the through hole, and the column is slidably connected to the lock tongue; the lock tongue sleeve is provided with a tension spring, one end of the tension spring is connected to the column, and the other end is connected to the lock tongue.

[0019] Preferably, the bottom end of the column is threadedly connected to the clamp.

[0020] Preferably, the clamp includes a sleeve and a pressure column, and the pressure column is slidably connected to the sleeve; the sleeve has a first transverse hole, and the pressure column has a second transverse hole; a compression spring is connected between the bottom end of the pressure column and the inner bottom of the sleeve; the pipeline passes through the first transverse hole and the second transverse hole; under the action of the elastic force of the compression spring, the first transverse hole and the second transverse hole are staggered to clamp the pipeline to form a closed circuit.

[0021] Preferably, the sleeve and the pressure column are made of plastic material.

[0022] Preferably, the locking member is made of plastic.

[0023] The above technical solution has the following advantages or beneficial effects:

[0024] 1. Large blood collection volume, large blood flow, high blood quality, and no pollution. The utility model adopts common materials such as syringes and infusion needles, and the required experimental equipment is simple and common; the configured clamp and locking piece can be obtained by simply modifying the syringe, and the modification is easy; when in use, since the mandibular part is rich in venous plexus, the syringe is in a negative pressure state. As long as the needle pierces the venous plexus, blood will flow from the needle into the barrel, which can effectively solve the problem of blood flowing out from the mandibular hair and sticking to the hair to contaminate the blood, as well as the problem of small blood collection volume; in addition, the scale value of the syringe is convenient for observation, and the blood collection volume can be controlled according to experimental needs.

[0025] 2. The clamp provided in the device can clamp and close the pipeline by providing a first transverse hole and a second transverse hole, and utilizing the elastic force of a compression spring to displace the first transverse hole and the second transverse hole. When the negative pressure state of the syringe is established, the hands of the staff can be freed, which is convenient for operation. In addition, the clamp is light in weight and does not affect the staff's needle insertion.

[0026] 3. The locking member in this device is provided with two structural forms, both of which have the advantages of simple structure, easy manufacture and installation, low cost for syringe modification, simple and practical. In addition, the device is easy to learn and can be mastered quickly. This method only requires the anesthesia of the mouse and the depth of the needle entering the body, and blood collection is easier to master. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. They are used together with the embodiments of the present invention to explain the present invention and do not constitute a limitation to the present invention.

[0028] Figure 1It is a structural stereogram of the utility model in the first embodiment (the clamping plate and the positioning groove are clamped together).

[0029] Figure 2 yes Figure 1 Structural stereogram of the syringe and the locking piece (the clamping plate and the positioning groove are not clamped).

[0030] Figure 3 yes Figure 1 Structural cross-sectional view of the middle locking member when it is engaged with the positioning groove.

[0031] Figure 4 It is a structural explosion diagram of the utility model in the second embodiment.

[0032] Figure 5 yes Figure 4 Schematic diagram of the structure when the middle locking member is engaged with the positioning groove.

[0033] Figure 6 yes Figure 4 A three-dimensional enlarged view of the structure of the middle locking part.

[0034] Figure 7 yes Figure 1 An enlarged view of the structure of the clamp occluder and the pipeline in the utility model.

[0035] Figure 8 yes Figure 7 Structural cross-sectional view of the middle clip occluder.

[0036] Description of reference numerals:

[0037] 1. Cylinder; 2. Plug rod; 3. Plug handle; 4. Flange; 5. Clamp; 51. Hook; 52. End cap; 6. Pipeline; 7. Needle; 8. Positioning groove; 9. Clip; 10. Column; 101. Through hole; 11. Lock tongue; 111. Tension spring; 12. Clamp; 121. Sleeve; 1211. First transverse hole; 122. Compression column; 1221. Second transverse hole; 123. Compression spring. DETAILED DESCRIPTION

[0038] In order to make the purpose, features and advantages of the utility model more obvious and easy to understand, the technical solutions in the embodiments of the utility model will be described clearly and completely in combination with the drawings in the embodiments of the utility model. Obviously, the embodiments described below are only part of the embodiments of the utility model, not all of them. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0039] It should be noted that, in the description of the present invention, terms such as "up", "down", "left", "right", "inside" and "outside" indicating directions or positional relationships are based on the directions or positional relationships shown in the drawings. This is merely for the convenience of description and does not indicate or imply that the device or element must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it cannot be understood as a limitation on the present invention.

[0040] In addition, it should be noted that in the description of the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For those skilled in the art, the specific meanings of the above terms in the present invention can be understood according to the specific circumstances.

[0041] Embodiment 1:

[0042] like Figure 1 - Figure 3 As shown, this embodiment proposes a blood collection device for constructing a SAKI model, including a syringe and an infusion needle. The syringe includes a barrel 1, a plug rod 2 and a piston. The tail end of the plug rod 2 is connected to a plug handle 3, which is a conventional structure; the plug rod 2 has four wing plates, forming a cross-shaped structure, and a receiving cavity is formed between adjacent wing plates. The infusion needle includes a needle 7 and a pipeline 6; one end of the pipeline 6 is connected to the needle 7, and the other end is connected to the nipple of the barrel 1. The entire device also includes a clamp 12 and a locking member, which are described in detail below:

[0043] Regarding the clamp 12: it is detachably arranged on the pipeline 6, and can realize the opening and closing of the pipeline 6, and control the opening or closing of the pipeline 6; the positioning groove 8, at least one of which is provided, is opened on at least one wing plate; the positioning groove 8 can be directly cut out on the wing plate, and its position and number are determined according to the test requirements.

[0044] Regarding the locking piece: it is connected to the cylinder body 1, and can be a detachable connection or a fixed connection; the locking piece can be adapted to be snap-fitted with the positioning groove 8, and after snap-fitting, the plug rod 2 can be positioned to prevent the plug rod 2 from moving along the axial direction, thereby playing a limiting role.

[0045] In addition, the clamp 12 is used in conjunction with the locking member, and after the plug rod 2 is pulled out, the syringe can form and maintain a negative pressure state. When in use, the clamp 12 is clamped on the pipeline 6 to clamp the pipeline 6 and close the pipeline 6; then the plug rod 2 is pulled out, and the piston moves in a direction away from the needle 7 under the drive of the plug rod 2 to form suction. At this time, a negative pressure state is formed in the syringe, and then the locking member is engaged with the positioning groove 8 on the plug rod 2 to achieve positioning and locking of the locking member, so that the negative pressure state can be ensured.

[0046] In this embodiment, the locking member may adopt the following structural form:

[0047] The locking member includes a card plate 5. The rear end of the cylinder 1 has a flange 4. One end of the card plate 5 is connected to the surface of the flange 4, and the other end extends into the accommodating chamber. The plug rod 2 and the piston can rotate relative to each other. The fixation between the card plate 5 and the flange 4 can be bolted, bonded, clamped, etc. The length of the card plate 5 should be moderate. After one end of the card plate 5 is extended into the accommodating chamber, it is necessary to ensure that the plug rod 2 does not touch the card plate 5 when it moves axially, so as not to affect the normal suction action. After pulling a certain distance, the plug rod 2 is rotated, and the positioning groove 8 also rotates with it. The positioning groove 8 can engage with the card plate 5 to axially lock and position the plug rod 2, so that the constructed negative pressure state can be maintained, otherwise the piston will reset once you let go.

[0048] In this embodiment, the clamping plate 5 and the flange 4 can be fixed by bolting as follows:

[0049] The flange 4 is provided with a positioning hole, and the clamping plate 5 is connected with a threaded column, and the clamping plate 5 and the threaded column can be fixed by gluing; one end of the threaded column passes through the positioning hole and is threadedly connected with a nut. During production, a pin hole is drilled on the flange 4 using a drill to form a positioning hole, and then the bottom end of the threaded column is passed through the positioning hole and the nut is screwed on to complete the connection. In this embodiment, in order to facilitate material collection and processing and production, the locking member is made of plastic material.

[0050] In this embodiment, the clamping plate 5 and the flange 4 may also be fixed by clamping, as follows:

[0051] Likewise, a positioning hole is formed in the flange 4 , and a hook 51 is connected to the clamping plate 5 . One end of the hook 51 passes through the positioning hole and is clamped with an end cap 52 .

[0052] Regarding the clip closure 12, common structures such as conventional clips and spring water-stopping clips can be used. However, considering that these existing devices are large in size and heavy in weight, after being set on the pipeline 6, the weight of the pipeline 6 is increased, and it is inconvenient for the staff to hold the needle 7 for puncture. For this reason, some improvements are made:

[0053] like Figure 7 - Figure 8As shown, in this embodiment, the clamp 12 includes a sleeve 121 and a pressure column 122. The cross section of the sleeve 121 is a U-shaped structure. The bottom end of the pressure column 122 is arranged in the sleeve 121 and can be slidably connected with the sleeve 121. The sleeve 121 has a first transverse hole 1211, and the pressure column 122 has a second transverse hole 1221. The first transverse hole 1211 matches the second transverse hole 1221. The bottom end of the pressure column 122 is connected to the inner bottom of the sleeve 121. There is a compression spring 123, by which the relative positions of the first transverse hole 1211 and the second transverse hole 1221 can be adjusted; the pipeline 6 passes through the first transverse hole 1211 and the second transverse hole 1221; under the elastic force of the compression spring 123, the first transverse hole 1211 and the second transverse hole 1221 are staggered to clamp the pipeline 6 to form a closed circuit; when the pressure column 122 is pressed, the compression spring 123 is compressed, and at this time the pipeline 6 is no longer clamped to form an open circuit.

[0054] In order to reduce weight, the sleeve 121 and the pressure column 122 are made of plastic. The clamp 12 provided in the device can clamp and close the pipeline 6 by providing the first transverse hole 1211 and the second transverse hole 1221, and using the elastic force of the compression spring 123 to make the first transverse hole 1211 and the second transverse hole 1221 misaligned. When the negative pressure state of the syringe is established, the hands of the staff can be freed, which is convenient for operation; in addition, the clamp 12 is light in weight and does not affect the staff's needle insertion.

[0055] Application results:

[0056] This scheme uses common materials such as syringes and infusion needles, and the required experimental equipment is simple and common; the clamp 12 and the locking piece are configured, and the syringe can be obtained by simply modifying the syringe, and the modification is easy. When in use, since the mandibular part is rich in venous plexus, the syringe is in a negative pressure state. As long as the needle 7 pierces the venous plexus, the blood will flow from the needle 7 into the barrel 1, which can effectively solve the problem of blood flowing out from the mandibular hair and sticking to the hair to contaminate the blood and the problem of small blood collection volume; using the negative pressure state, suction can be achieved, and the blood collection volume and blood flow are large; using the infusion needle 7, it can also ensure that the collected blood is of high quality and pollution-free; in addition, the scale value provided by the syringe is convenient for observation, and the blood collection volume can be controlled according to experimental needs.

[0057] Embodiment 2:

[0058] like Figure 4 - Figure 6 As shown, other structures are the same as those in the first embodiment, except that the locking member can also adopt the following structural form:

[0059] Considering that the installation of the above locking parts requires drilling, which is troublesome, further optimization is made for this purpose:

[0060] In this embodiment, the locking member includes a clamp 9, which is in an "Ω" shape as a whole and has slight elasticity. The clamp 9 can be detachably clamped on the cylinder 1. A column 10 is provided on the clamp 9, and a locking tongue 11 is connected to the column 10, and the locking tongue 11 can be adapted and engaged with the positioning groove 8.

[0061] There are two ways to set up the structure of the lock tongue 11:

[0062] Firstly, the locking tongue 11 is a fixed structure, the locking tongue 11 is directly fixedly connected to the column 10, the clamp 9 is clamped on the cylinder 1, and the bottom surface of the locking tongue 11 is pressed against the upper surface of the flange 4 to prevent it from sliding down, thus playing a limiting role; normally, one end of the locking tongue 11 is also extended into the accommodating cavity, which does not affect the circumferential displacement of the plug rod 2; when positioning is required, the plug rod 2 is rotated to clamp the locking tongue 11 into the positioning groove 8.

[0063] Secondly, the lock tongue 11 is a movable structure, the column 10 is provided with a through hole 101, one end of the lock tongue 11 passes through the through hole 101, and the column 10 is slidably connected with the lock tongue 11; the lock tongue 11 is sleeved with a tension spring 111, one end of the tension spring 111 is connected to the column 10, and the other end is connected to the lock tongue 11. When the plug rod 2 is pulled out, one end of the lock tongue 11 is pinched and pulled, at which time the lock tongue 11 is away from the plug rod 2, and the tension spring 111 generates tension; when the plug rod 2 needs to be positioned after being pulled out, the lock tongue 11 is released, and under the elastic force of the tension spring 111, the lock tongue 11 is reset and automatically inserted into the positioning groove 8, so that the plug rod 2 is clamped and positioned.

[0064] In this embodiment, the bottom end of the column 10 and the clamp 9 can be connected by thread.

[0065] It can be seen from the above embodiments that the locking member in the device is provided with two structural forms, either of which has the advantages of simple structure, easy manufacture and installation, low cost for syringe modification, simple and practical. In addition, the device is easy to learn and can be mastered quickly. The method only needs to do a good job of anesthetizing the mouse and the depth of the needle 7 entering the body, and blood collection is easier to master.

[0066] Practical Application:

[0067] 1-1. Animal grouping and treatment:

[0068] 8-10 weeks old SPF male 1257BL / 6 mice were randomly divided into 5 groups. Mice in each group were selected at 0h, 6h, 12h, and 24h after modeling. Blood was collected at each time point to detect serum creatinine (12r) and serum urea nitrogen (BUN), and urine was collected to detect urine NGAL and KIM-1 levels. After 24h of blood collection, the mice were killed and serum and kidney tissues were taken for relevant experimental analysis. The remaining 4 mice in each group were observed for biological behavior and 7-day survival analysis.

[0069] 1-2. Establishment of sepsis-induced acute kidney injury model:

[0070] Weigh the animals, and determine the dosage of anesthetic drugs (2% sodium pentobarbital 45 mg / kg) according to the weight of the mice for intraperitoneal injection. Use forceps to stimulate the toes of the mice to monitor the intensity of anesthesia. No response to stimulation of the limbs indicates adequate anesthesia. Trim the abdomen of the mouse with a shaver, and disinfect it with alcohol and iodine after trimming. Make a longitudinal incision on the skin with a scalpel, be careful not to pierce the abdominal cavity, and use small scissors to extend the incision (1.5-212m). Cut the fascia and peritoneal layers, use blunt dissecting forceps to locate the cecum, separate the cecum and pull it out of the body, and ligate the 1 / 2 length of the cecum. For sham-operated animals, the intestine is not ligated. Before cecal perforation, gently push the cecal contents to the blind end, and perforate the intestine between the cecal ligation and the tip of the cecum, and avoid piercing the blood vessels. Remove the puncture needle and squeeze out a small amount of feces from both sides of the intestinal perforation to ensure patency. Keep the amount of cecal feces squeezed out the same each time to ensure consistency in modeling. Suture the peritoneum, fascia, and abdominal muscles continuously, and suture the skin interruptedly. Fluid resuscitation was performed by subcutaneous injection of preheated saline (37°C; 5 ml / 100 g). The mice were returned to their cages with free access to food and water. They were placed in a 12-hour light and dark alternating environment with controlled temperature (22 ± 1°C).

[0071] 1-3. Blood collection:

[0072] When drawing blood, the above-mentioned blood collection device is used to collect blood.

[0073] Required test equipment: disposable intravenous infusion needle (No. 5.5) and 2.5mL syringe as well as clamp 12 and locking piece.

[0074] The blood collection method uses negative pressure blood collection from the submandibular venous plexus: put the intravenous infusion needle on the 2.5mL intravenous syringe, clamp the tube 6 of the infusion needle with the clamp 12, gently guide the syringe stopper 2, draw the syringe into a negative pressure vacuum state, and use the locking piece to position and lock the stopper 2. Insert the needle into the skin at the top of the left (or right) upper limb of the mouse, with the needle 7 facing the direction of the mouse's eye corner. When the needle has completely entered the muscle, try to slowly advance or withdraw the needle in the mandibular part. When it touches the submandibular venous plexus, blood will flow into the hose of the infusion needle. At this time, the amount of blood to be drawn can be determined according to needs.

[0075] End of blood collection: pinch one end of the needle 7 with one hand, pull out the needle 7, put it into a sterilized centrifuge tube, and then introduce the blood into the centrifuge tube to complete the blood sample collection.

[0076] In the experiment, the collection of blood samples is a key link. If a sufficient amount of blood cannot be obtained, the experimental results will be directly affected. After the blood sampling operation in this experiment, the wound was small and healed quickly. The skin was pierced with a needle 7, and the damage caused was very small, which had almost no effect on the animal. Blood sampling could be repeated many times, which complied with the animal welfare principle, and no obvious abnormal behavior and mental depression were observed in the mice. They all quickly recovered to an active state, and all experimental mice survived normally, meeting the requirements of the experiment.

[0077] In order to improve the success rate of blood collection, the following points should be noted: 1) The mouse should be completely anesthetized before blood collection, otherwise the mouse may easily cause problems such as blood vessel displacement or needle 7 falling off during the swinging process, making blood collection difficult. 2) The size of the needle 7 of the intravenous infusion device for blood collection, the force and direction of the needle should be determined according to the body weight of the mouse, and the operator needs to go through many practices and training to master it well. 3) The blood collection process generally follows the principle of aseptic operation. Before blood collection, the needle insertion site must be wiped with an alcohol cotton ball. On the one hand, it can be disinfected, and on the other hand, it can be well mastered.

[0078] Although the specific implementation methods of the utility model are described above in conjunction with the accompanying drawings, this does not limit the scope of protection of the utility model. On the basis of the technical solution of the utility model, various modifications or deformations that can be made by technical personnel in this field without creative work are still within the scope of protection of the utility model.

Claims

1. A blood collection device for constructing a SAKI model, comprising a syringe and an infusion needle, wherein the syringe comprises a barrel (1), a stopper rod (2) and a piston, wherein the stopper rod (2) has four wing plates to form a cross-shaped structure, and a receiving cavity is formed between adjacent wing plates; the infusion needle comprises a needle (7) and a pipeline (6); one end of the pipeline (6) is connected to the needle (7), and the other end is connected to the nipple of the barrel (1); characterized in that: Also includes: A clamp (12) detachably arranged on the pipeline (6) and capable of opening and closing the pipeline (6); A positioning groove (8), at least one of which is provided and is disposed on at least one of the wing plates; A locking member is detachably connected to the cylinder (1); the locking member can be adapted to engage with the positioning groove (8) to achieve positioning of the plug rod (2); The clamp (12) is used in conjunction with the locking member to enable the syringe to form and maintain a negative pressure state after the plug rod (2) is pulled out.

2. The blood collection device for constructing the SAKI model according to claim 1, characterized in that: The locking member comprises a clamping plate (5), the rear end of the cylinder (1) having a flange (4), one end of the clamping plate (5) being connected to the surface of the flange (4), and the other end extending into the accommodating cavity; the plug rod (2) and the piston are relatively rotatable; when the plug rod (2) is rotated, the positioning groove (8) can engage with the clamping plate (5), thereby axially locking and positioning the plug rod (2).

3. The blood collection device for constructing the SAKI model according to claim 2, characterized in that: The flange (4) is provided with a positioning hole, the clamping plate (5) is connected with a threaded column, and one end of the threaded column passes through the positioning hole and is threadedly connected with a nut.

4. The blood collection device for constructing the SAKI model according to claim 2, characterized in that: The flange (4) is provided with a positioning hole, the clamping plate (5) is connected with a clamping hook (51), and one end of the clamping hook (51) passes through the positioning hole and is clamped with an end cap (52).

5. The blood collection device for constructing the SAKI model according to claim 1, characterized in that: The locking member comprises a clamp (9), the clamp (9) being detachably clamped on the cylinder (1), the clamp (9) being provided with a column (10), the column (10) being connected with a locking tongue (11), and the locking tongue (11) being adaptable and clamped with the positioning groove (8).

6. The blood collection device for constructing the SAKI model according to claim 5, characterized in that: The column (10) is provided with a through hole (101), one end of the lock tongue (11) passes through the through hole (101), and the column (10) is slidably connected to the lock tongue (11); the lock tongue (11) is sleeved with a tension spring (111), one end of the tension spring (111) is connected to the column (10), and the other end is connected to the lock tongue (11).

7. The blood collection device for constructing the SAKI model according to claim 5, characterized in that: The bottom end of the column (10) is threadedly connected to the clamp (9).

8. The blood collection device for constructing the SAKI model according to claim 1, characterized in that: The clamp (12) comprises a sleeve (121) and a pressure column (122), wherein the pressure column (122) is slidably connected to the sleeve (121); the sleeve (121) has a first transverse hole (1211), and the pressure column (122) has a second transverse hole (1221); a compression spring (123) is connected between the bottom end of the pressure column (122) and the inner bottom of the sleeve (121); the pipeline (6) passes through the first transverse hole (1211) and the second transverse hole (1221); under the elastic force of the compression spring (123), the first transverse hole (1211) and the second transverse hole (1221) are staggered to clamp the pipeline (6) to form a closed circuit.

9. The blood collection device for constructing the SAKI model according to claim 8, characterized in that: The sleeve (121) and the pressure column (122) are made of plastic material.

10. The blood collection device for constructing the SAKI model according to claim 3, characterized in that: The locking piece is made of plastic material.

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