Bioreactor cleaning system

Abstract

The utility model relates to the technical field of biological pharmacy, in particular to a bioreactor cleaning system. The bioreactor cleaning system comprises a circulating liquid supply tank, and a water supply pipeline and a cleaning liquid output port are arranged on the circulating liquid supply tank; one end of the cleaning liquid conveying pipe is communicated with the cleaning liquid output port, and a cleaning liquid conveying pump is installed on the cleaning liquid conveying pipe; the return pipe is communicated with the circulating liquid supply tank through a return branch pipe, and a return pump is mounted on the return pipe; the discharge pipeline is communicated with the other end of the return pipe; the circulating pipeline is communicated with the cleaning liquid conveying pipe and the return pipe; the concentrated alkali tank is communicated with the circulating liquid supply tank through an alkali liquid conveying pipe, and an alkali liquid conveying pump is mounted on the alkali liquid conveying pipe; the concentrated acid tank is communicated with the circulating liquid supply tank through an acid liquid conveying pipe, and an acid liquid conveying pump is mounted on the acid liquid conveying pipe. According to the system, oil stains or sediments in the bioreactor can be effectively removed through alkali liquor or acid liquor, and the cleaning effect is effectively improved.

Description

Technical Field

[0001] The present application relates to the field of biopharmaceutical technology, and in particular to a bioreactor cleaning system. Background Art

[0002] In the biopharmaceutical industry, bioreactors are one of the most important production equipment, and they play an important role in material transfer and storage. However, after the bioreactor is used for material transfer or storage, if it is not cleaned or the cleaning is not thorough, it will directly contaminate the material and affect the production quality and efficiency. This makes it very important to clean the bioreactor in production operations. In the prior art, bioreactors are usually cleaned directly with clean water, but clean water cannot effectively remove oil or sediment in the reactor. Over time, more dirt will gradually accumulate in the reactor, so the cleaning effect is not good. Summary of the invention

[0003] The present application aims to solve at least one of the above technical problems in the prior art to a certain extent. To this end, the present application provides a bioreactor cleaning system, which can effectively remove oil stains or sediments inside the bioreactor by alkaline or acidic solution, effectively improving the cleaning effect.

[0004] A bioreactor cleaning system, comprising:

[0005] A circulating liquid supply tank, wherein the circulating liquid supply tank is provided with a water supply pipeline and a cleaning liquid output port;

[0006] A cleaning liquid delivery pipe, one end of which is connected to the cleaning liquid outlet, and the other end of which is used to connect to the bioreactor to be cleaned, and a cleaning liquid delivery pump is installed on the cleaning liquid delivery pipe;

[0007] A reflux pipe, the reflux pipe is connected to the circulating liquid supply tank through a reflux branch pipe, one end of the reflux pipe is used to be connected to the bioreactor to be cleaned, and a reflux pump is installed on the reflux pipe;

[0008] a discharge pipeline, the discharge pipeline being connected to the other end of the reflux pipe;

[0009] A circulation pipeline, the circulation pipeline connecting the cleaning liquid delivery pipe and the reflux pipe;

[0010] A concentrated alkali tank, the concentrated alkali tank is connected to the circulating liquid supply tank through an alkali liquid delivery pipe, and an alkali liquid delivery pump is installed on the alkali liquid delivery pipe;

[0011] A concentrated acid tank is connected to the circulating liquid supply tank through an acid delivery pipe, and an acid delivery pump is installed on the acid delivery pipe.

[0012] In an optional or preferred embodiment, an alkali addition tank is installed on the alkali solution delivery pipe, and the alkali addition tank is located between the concentrated alkali tank and the circulating liquid supply tank.

[0013] In an optional or preferred embodiment, an acid addition tank is installed on the acid liquid delivery pipe, and the acid addition tank is located between the concentrated acid tank and the circulating liquid supply tank.

[0014] In an optional or preferred embodiment, the water pipeline and the purified water pipeline, the injection water pipeline and the purified water pipeline extend from the top of the circulating liquid supply tank into the circulating liquid supply tank.

[0015] In an optional or preferred embodiment, a spherical nozzle is provided at one end of the injection water pipeline and the purified water pipeline extending into the circulating liquid supply tank.

[0016] In an optional or preferred embodiment, the reflux pipe is connected to the circulating liquid supply tank via a reflux cleaning branch pipe, and a spherical nozzle is provided at one end of the reflux cleaning branch pipe extending into the interior of the circulating liquid supply tank.

[0017] In an optional or preferred embodiment, a tubular heat exchanger is installed on the cleaning liquid delivery pipe, the inlet of the tubular heat exchanger is connected to a steam pipe, and the outlet of the tubular heat exchanger is connected to a condensate water pipe.

[0018] In an optional or preferred embodiment, a spherical nozzle is provided at one end of the cleaning liquid delivery pipe extending into the bioreactor to be cleaned.

[0019] In an optional or preferred embodiment, a compressed air pipeline is installed on the cleaning liquid delivery pipe.

[0020] In an optional or preferred embodiment, the drain outlet of the reflux pump and the drain outlet of the cleaning liquid delivery pump are both connected to the discharge pipeline.

[0021] Based on the above technical scheme, the embodiments of the present application have at least the following beneficial effects: In the above technical scheme, during the clean water cleaning process, the water supply pipeline accumulates water in the circulating liquid supply tank, and then the cleaning liquid delivery pump pumps the clean water through the cleaning liquid delivery pipe to the bioreactor to be cleaned, thereby cleaning the bioreactor to be cleaned. When alkali solution is needed for cleaning, the alkali solution is first prepared, and water is accumulated in the circulating liquid supply tank through the water supply pipeline. After the water is accumulated, the concentrated alkali tank is opened, and the alkali solution delivery pump pumps the alkali solution through the alkali solution delivery pipe to the circulating liquid supply tank to mix with the clean water. At the same time, the cleaning liquid delivery pump also starts working to pump the alkali solution in the circulating liquid supply tank. The alkali solution and clean water are extracted and pumped back into the circulating liquid supply tank through the circulation pipeline and the reflux branch pipe, so that the alkali solution and clean water are evenly mixed. When the alkali solution is prepared, the cleaning liquid delivery pump pumps the prepared alkali solution in the circulating liquid supply tank through the cleaning liquid delivery pipe to the bioreactor to be cleaned, thereby cleaning the bioreactor to be cleaned with alkali solution. When acid cleaning is required, the cleaning steps are the same as the above-mentioned alkali cleaning steps. After the alkali solution or acid cleaning is completed, the bioreactor to be cleaned is cleaned with clean water to remove the alkali solution or acid solution in the bioreactor to be cleaned. The clean water cleaning steps are the same as the above-mentioned clean water cleaning process. This system can effectively remove oil or sediment inside the bioreactor to be cleaned with alkali solution or acid solution, effectively improving the cleaning effect. The cleaned water in the bioreactor to be cleaned is extracted from the bioreactor to be cleaned by the reflux pump and transported to the discharge pipeline through the reflux pipe for discharge. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The present application is further described below with reference to the accompanying drawings and embodiments;

[0023] Figure 1 Schematic diagram of a bioreactor cleaning system provided in an embodiment of the present application. DETAILED DESCRIPTION

[0024] In order to enable those skilled in the art to better understand the technical solutions in the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in the field without creative work should fall within the scope of protection of the present application.

[0025] The following is a further detailed description of the implementation of the present application in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present application but cannot be used to limit the scope of the present application.

[0026] In the description of the embodiments of the present application, it should be noted that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, which are only for the convenience of describing the embodiments of the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the embodiments of the present application. In addition, the terms "first", "second", and "third" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0027] In the description of the embodiments of the present application, it should be noted that, unless otherwise clearly specified and limited, the terms "connected" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the embodiments of the present application can be understood according to specific circumstances.

[0028] In the embodiments of the present application, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" and "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0029] In the biopharmaceutical industry, bioreactors are one of the most important production equipment, and they play an important role as material transfer and storage. However, after the bioreactor is used for material transfer or storage, if it is not cleaned or the cleaning is not thorough, it will directly contaminate the material and affect the production quality and efficiency. This makes it very important to clean the bioreactor in production operations. In the prior art, bioreactors and their pipelines are usually cleaned directly with clean water, but direct cleaning with clean water cannot effectively remove oil or sediment in the reactor and pipeline. Over time, more dirt will gradually accumulate in the reactor and pipeline, so the cleaning effect is not good.

[0030] Reference Figure 1 The present application provides a bioreactor cleaning system, including a circulating liquid supply tank 1, a cleaning liquid delivery pipe 2, a reflux pipe 3, a discharge pipeline 4, a circulating pipeline 5, a concentrated alkali tank 6 and a concentrated acid tank 7.

[0031] A water supply pipeline 8 and a cleaning liquid outlet are provided on the circulating liquid supply tank 1. One end of the cleaning liquid delivery pipe 2 is connected to the cleaning liquid outlet, and the other end of the cleaning liquid delivery pipe 2 is used to connect to the bioreactor 9 to be cleaned. A cleaning liquid delivery pump 20 is installed on the cleaning liquid delivery pipe 2. The reflux pipe 3 is connected to the circulating liquid supply tank 1 through a reflux branch pipe 31. One end of the reflux pipe 3 is used to connect to the bioreactor 9 to be cleaned. The reflux pump 30 is installed on the reflux pipe 3. The discharge pipeline 4 is connected to the other end of the reflux pipe 3. The circulating pipeline 5 connects the cleaning liquid delivery pipe 2 and the reflux pipe 3. The concentrated alkali tank 6 is connected to the circulating liquid supply tank 1 through an alkali delivery pipe 60. An alkali delivery pump 600 is installed on the alkali delivery pipe 60. The concentrated acid tank 7 is connected to the circulating liquid supply tank 1 through an acid delivery pipe 70. An acid delivery pump 700 is installed on the acid delivery pipe 70.

[0032] During the clean water cleaning process, the water supply pipeline 8 stores water in the circulating liquid supply tank 1, and then the cleaning liquid delivery pump 20 pumps the clean water to the bioreactor to be cleaned 9 through the cleaning liquid delivery pipe 2, so as to clean the bioreactor 9 to be cleaned. When alkali solution is needed for cleaning, the alkali solution is first prepared and water is stored in the circulating liquid supply tank 1 through the water supply pipeline 8. After the water storage is completed, the concentrated alkali tank 6 is opened, and the alkali solution delivery pump 600 pumps the alkali solution through the alkali solution delivery pipe 60 to the circulating liquid supply tank 1 to mix with the clean water. At the same time, the cleaning liquid delivery pump 20 also starts to work, and the alkali solution and clean water in the circulating liquid supply tank 1 are pumped out through the circulation pipeline 5 and the return line 60. The branch pipe 31 is pumped back into the circulating liquid supply tank 1 so that the alkali solution and the clean water are evenly mixed. When the alkali solution is prepared, the cleaning liquid delivery pump 20 pumps the prepared alkali solution in the circulating liquid supply tank 1 through the cleaning liquid delivery pipe 2 to the bioreactor 9 to be cleaned, so as to clean the bioreactor 9 with alkali solution. When acid cleaning is required, the cleaning steps are the same as the above-mentioned alkali cleaning steps. After the alkali solution or acid cleaning is completed, the bioreactor 9 to be cleaned is cleaned with clean water to remove the residual alkali solution or acid solution in the bioreactor 9 to be cleaned. The clean water cleaning steps are the same as the above-mentioned clean water cleaning process. In this way, the oil or sediment inside the bioreactor 9 to be cleaned can be effectively removed by alkali solution cleaning or acid solution cleaning, and the cleaning effect is effectively improved. The cleaned water in the bioreactor 9 to be cleaned is extracted from the bioreactor 9 to be cleaned by the reflux pump 30 and transported to the discharge pipeline 4 through the reflux pipe 3 for discharge.

[0033] A valve is installed on the circulation pipeline 5, a valve is installed on the reflux pipe 3 near the bottom of the bioreactor 9 to be cleaned, a valve is installed on the reflux pipe 3 near the circulation pipeline 5, a valve is installed on the reflux pipe 3 near the drain outlet of the reflux pipe 3, and a valve is installed on the reflux branch pipe 31.

[0034] In some embodiments, the water supply pipeline 8 includes an injection water pipeline 80 and a purified water pipeline 81, and the injection water pipeline 80 and the purified water pipeline 81 extend from the top of the circulating liquid supply tank 1 into the circulating liquid supply tank 1. When purified water is needed for cleaning, purified water is directly supplied to the circulating liquid supply tank 1 through the purified water pipeline 81 for cleaning, and when injection water is needed for cleaning, injection water is supplied to the circulating liquid supply tank 1 through the injection water pipeline 80 for cleaning.

[0035] The injection water pipeline 80 is provided with a valve for controlling the opening and closing of the injection water pipeline 80 , and the purified water pipeline 81 is provided with a valve for controlling the opening and closing of the purified water pipeline 81 .

[0036] In some embodiments, a spherical nozzle 10 is provided at one end of the injection water pipeline 80 and the purified water pipeline 81 extending into the circulating liquid supply tank 1. In this way, the mixing effect of clean water and alkali solution or acid solution is better during the preparation of alkali solution or acid solution.

[0037] In some embodiments, an alkali adding tank 601 is installed on the alkali liquid delivery pipe 60, and the alkali adding tank 601 is located between the concentrated alkali tank 6 and the circulating liquid supply tank 1. The alkali adding tank 601 is used to add alkali material to increase the concentration of the alkali liquid preparation. A valve for controlling the opening and closing of the alkali liquid delivery pipe 60 is provided on the alkali liquid delivery pipe 60.

[0038] In some embodiments, an acid adding tank 701 is installed on the acid delivery pipe 70, and the acid adding tank 701 is located between the concentrated acid tank 7 and the circulating liquid supply tank 1. The acid adding tank 701 is used to add acid material to increase the concentration of the acid preparation. A valve for controlling the switch of the acid delivery pipe 70 is provided on the acid delivery pipe 70.

[0039] In some embodiments, the reflux pipe 3 is connected to the circulating liquid supply tank 1 through the reflux cleaning branch pipe 32, and a spherical nozzle 10 is provided at one end of the reflux cleaning branch pipe 32 extending into the inside of the circulating liquid supply tank 1. When the circulating liquid supply tank 1 needs to be cleaned, water is stored in the circulating liquid supply tank 1 through the water supply pipe 8, and then the cleaning liquid delivery pump 20 draws clean water from the circulating liquid supply tank 1 through the cleaning liquid delivery pipe 2, the circulation pipe 5 and the reflux cleaning branch pipe 32, and then sprays the clean water onto the inner wall of the circulating liquid supply tank 1 through the spherical nozzle 10, so as to flush and clean the circulating liquid supply tank 1.

[0040] A valve is installed on the reflux cleaning branch pipe 32 to control the opening and closing of the reflux cleaning branch pipe 32 .

[0041] In order to improve the cleaning effect, a tubular heat exchanger 21 is installed on the cleaning liquid delivery pipe 2, the inlet of the tubular heat exchanger 21 is connected to the steam pipe 210, and the outlet of the tubular heat exchanger 21 is connected to the condensed water pipe 211. The cleaning liquid in the cleaning liquid delivery pipe 2 can be heated by the tubular heat exchanger 21, and the cleaning effect of the cleaning liquid after heating is better. The condensed water after heating is discharged through the condensed water pipe 211.

[0042] An air filter and a diaphragm valve are installed on the steam pipe 210 . The diaphragm valve is used to control the switch of compressed air, and the air filter is used to filter air. A drain valve is installed on the condensate water pipe 211 .

[0043] In some embodiments, a spherical nozzle 10 is provided at one end of the cleaning liquid delivery pipe 2 extending into the bioreactor 9 to be cleaned. After the cleaning liquid passes through the spherical nozzle 10, the spraying area is larger, and the bioreactor 9 to be cleaned can be cleaned in all directions.

[0044] In some embodiments, a compressed air pipeline 22 is installed on the cleaning liquid delivery pipe 2. After cleaning is completed, compressed air is passed through the compressed air pipeline 22 to the cleaning liquid delivery pipe 2 to purge the liquid in each pipeline and the cleaning bioreactor 9.

[0045] A valve for controlling the switch is installed on the compressed air pipeline 22 .

[0046] In some embodiments, the drain outlet of the cleaning liquid delivery pump 20 and the drain outlet of the reflux pump 30 are both connected to the discharge pipeline 4 .

[0047] During the process of discharging the liquid, the cleaning liquid delivery pump 20 draws out the liquid in the circulating cleaning tank and discharges it through the discharge pipeline 4 , and the accumulated water at the bottom of the reflux pump 30 is discharged from the discharge pipeline 4 through the drain port of the reflux pump 30 .

[0048] In some embodiments, a pressure sensor 23, a temperature sensor 24, a conductivity sensor 25 and a flow meter 26 are installed on the cleaning liquid delivery pipe 2. The conductivity sensor 25, the temperature sensor 24, the pressure sensor 23 and the flow meter 26 are used to monitor and control the cleaning state in real time.

[0049] In addition, a conductivity sensor 25 is also provided at the drain outlet of the reflux pipe 3 to detect the conductivity of the cleaning liquid after cleaning.

[0050] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the embodiments of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0051] The embodiments of the present application are described in detail above in conjunction with the accompanying drawings, but the present application is not limited to the above embodiments, and various changes can be made within the knowledge scope of ordinary technicians in the technical field without departing from the purpose of the present application.

Claims

1. A bioreactor cleaning system, characterized in that ,include: A circulating liquid supply tank, wherein the circulating liquid supply tank is provided with a water supply pipeline and a cleaning liquid output port; A cleaning liquid delivery pipe, one end of which is connected to the cleaning liquid outlet, and the other end of which is used to connect to the bioreactor to be cleaned, and a cleaning liquid delivery pump is installed on the cleaning liquid delivery pipe; A reflux pipe, the reflux pipe is connected to the circulating liquid supply tank through a reflux branch pipe, one end of the reflux pipe is used to be connected to the bioreactor to be cleaned, and a reflux pump is installed on the reflux pipe; a discharge pipeline, the discharge pipeline being connected to the other end of the reflux pipe; A circulation pipeline, the circulation pipeline connecting the cleaning liquid delivery pipe and the reflux pipe; A concentrated alkali tank, the concentrated alkali tank is connected to the circulating liquid supply tank through an alkali liquid delivery pipe, and an alkali liquid delivery pump is installed on the alkali liquid delivery pipe; A concentrated acid tank is connected to the circulating liquid supply tank through an acid delivery pipe, and an acid delivery pump is installed on the acid delivery pipe.

2. The bioreactor cleaning system according to claim 1, characterized in that: An alkali adding tank is installed on the alkali solution conveying pipe, and the alkali adding tank is located between the concentrated alkali tank and the circulating liquid supply tank.

3. The bioreactor cleaning system according to claim 2, characterized in that: An acid adding tank is installed on the acid liquid conveying pipe, and the acid adding tank is located between the concentrated acid tank and the circulating liquid supply tank.

4. The bioreactor cleaning system according to claim 1, characterized in that: The water supply pipeline includes an injection water pipeline and a purified water pipeline, and the injection water pipeline and the purified water pipeline extend from the top of the circulating liquid supply tank into the circulating liquid supply tank.

5. The bioreactor cleaning system according to claim 4, characterized in that: One end of the injection water pipeline and the purified water pipeline extending into the circulating liquid supply tank is provided with a spherical nozzle.

6. The bioreactor cleaning system according to claim 1, characterized in that: The reflux pipe is connected with the circulating liquid supply tank through a reflux cleaning branch pipe, and a spherical nozzle is arranged at one end of the reflux cleaning branch pipe extending into the circulating liquid supply tank.

7. The bioreactor cleaning system according to claim 1, characterized in that: A tubular heat exchanger is installed on the cleaning liquid delivery pipe, the inlet of the tubular heat exchanger is connected to the steam pipeline, and the outlet of the tubular heat exchanger is connected to the condensate pipeline.

8. The bioreactor cleaning system according to claim 1, characterized in that: The end of the cleaning liquid delivery pipe extending into the bioreactor to be cleaned is provided with a spherical nozzle.

9. The bioreactor cleaning system according to claim 1, characterized in that: A compressed air pipeline is installed on the cleaning liquid delivery pipe.

10. The bioreactor cleaning system according to claim 1, characterized in that: The drain outlet of the reflux pump and the drain outlet of the cleaning liquid delivery pump are both communicated with the discharge pipeline.

Images