DNA (Deoxyribose Nucleic Acid) purification device

Abstract

The utility model discloses a DNA (deoxyribonucleic acid) purification device, which relates to the field of DNA purification and comprises a shell, an annular plate is horizontally and rotatably arranged in the shell, and a rotating component is arranged in the shell and used for rotating the annular plate; a plurality of placing tubes for placing centrifugal tubes are rotationally arranged on the annular plate, and are circumferentially distributed on the annular plate by taking the axis of the annular plate as the center. The centrifugal separation device has the beneficial effects that the posture of the containing pipe is adjusted to be horizontal through the adjusting mechanism, the annular plate is rotated through the rotating assembly, the annular plate drives the containing pipe to rotate, and under the action of centrifugal force, substances with large density in the centrifugal pipe can rapidly move to one end of the centrifugal pipe, so that centrifugal separation is rapidly completed; the closed ends of all the placing pipes incline downwards through the adjusting mechanism, so that the situation that the placing pipes are still in a horizontal state after the centrifugal force is lost, and separated substances in the centrifugal pipe are mixed again can be avoided, the separation efficiency is improved, and the separation and purification quality is guaranteed.

Description

Technical Field

[0001] The utility model relates to the technical field of DNA purification, in particular to a DNA purification device. Background Art

[0002] In biological experiments, the purpose of DNA centrifugation is usually to separate DNA from other substances and purify it for further research.

[0003] The specific operation steps of centrifugal purification are to place the centrifuge tube in a centrifuge, and drive the centrifuge tube to rotate through the centrifuge to separate the substances inside the centrifuge tube;

[0004] In the prior art, centrifuge tubes are generally placed in centrifuges at an angle. The purpose of this design is that the angle can improve the effect of centrifugal force on the material compared to vertical, and can also use gravity to ensure that the material with higher density is still at the bottom of the centrifuge tube when the centrifuge stops.

[0005] However, the tilted placement of the centrifuge tubes greatly limits the separation effect of the centrifuge. The best way is to place the centrifuge tubes horizontally so that the length direction of the centrifuge tubes is arranged along the radial direction of the rotation trajectory. However, the problem with placing the centrifuge tubes in this way is that after the centrifuge stops, the separated substances are easy to merge again, and the existing centrifuges cannot solve this problem, so the centrifuge tubes are placed at an angle, but this reduces the separation effect. Utility Model Content

[0006] The purpose of the present invention is to provide a DNA purification device in order to solve the above problems, as described below.

[0007] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0008] The utility model provides a DNA purification device, comprising a shell, a ring plate is horizontally rotatably arranged in the shell, and a rotating assembly is arranged in the shell for rotating the ring plate;

[0009] The ring plate is rotatably provided with a plurality of placement tubes for placing centrifuge tubes, and the plurality of placement tubes are circumferentially distributed on the ring plate with the axis of the ring plate as the center;

[0010] An adjusting mechanism is arranged in the shell body for adjusting the horizontality of the placement tube.

[0011] The above-mentioned DNA purification device is used to place a centrifuge tube in a placement tube, and the posture of the placement tube is adjusted to be horizontal through an adjustment mechanism, and the sealing cover is placed on the shell. Then, the ring plate is rotated by a rotating assembly, and the placement tube is driven to rotate through the ring plate. Under the action of centrifugal force, the material with high density in the centrifuge tube will quickly move to one end of the centrifuge tube;

[0012] Before the ring plate stops rotating, the closed ends of all the placement tubes are tilted downward by the adjustment mechanism, so as to avoid the placement tubes being in a horizontal state after the centrifugal force is lost, resulting in the re-mixing of the separated substances in the centrifuge tubes.

[0013] Preferably, the shell is a circular cylinder, and a sealing cover is threadedly connected to the upper side of the shell.

[0014] Preferably, a plurality of ventilation holes are provided at the bottom of the shell, and a plurality of supporting feet are provided at the bottom of the shell.

[0015] Preferably, the rotating assembly comprises a gear ring, which is fixedly arranged at the bottom of the ring plate. A motor is arranged in the housing, and a gear is fixed on the output shaft of the motor, and the gear is meshed with the gear ring.

[0016] Preferably, the center of the gear ring is on the axis of the ring plate.

[0017] Preferably, the placement tube is rotatably arranged on the upper surface of the ring plate through a support frame.

[0018] Preferably, several of the placement tube openings are opposite to each other.

[0019] Preferably, the adjusting mechanism comprises a plurality of telescopic rods 1, which are vertically arranged on the ring plate, and the number of the telescopic rods 1 is consistent with the placement tube, a pulley is arranged at the movable end of the telescopic rod 1, and the pulley contacts the bottom of the placement tube, a spring 1 is sleeved on the surface of the telescopic rod 1, and the two ends of the spring 1 are respectively fixed to the movable end and the fixed end of the telescopic rod 1, a movable plate is slidably arranged at the hollow center of the ring plate, and two telescopic rods 2 are vertically fixed to the upper side of the ring plate through two fixing frames, the movable end of the telescopic rod 2 is fixed to the movable plate, a spring 2 is sleeved on the surface of the telescopic rod 2, and the two ends of the spring 2 are respectively fixed to the two ends of the telescopic rod 2, a number of top rods consistent with the placement tube is arranged on the movable plate, and the top rods correspond to the bottom of the placement tube, an electric push rod is vertically arranged on the bottom of the inner wall of the shell, a support block is rotatably arranged on the movable end of the electric push rod, and the support block corresponds to the bottom of the movable plate.

[0020] The beneficial effects are:

[0021] The posture of the placement tube is adjusted to be horizontal through the adjustment mechanism, and the ring plate is rotated by the rotating assembly, which drives the placement tube to rotate. Under the action of centrifugal force, the material with high density in the centrifuge tube will quickly move to one end of the centrifuge tube, thereby quickly completing the centrifugal separation. Before the ring plate stops rotating, the closed ends of all the placement tubes are tilted downward through the adjustment mechanism. This can avoid the placement tube being in a horizontal state after the centrifugal force is lost, resulting in the re-mixing of the separated materials in the centrifuge tube, thereby improving the separation efficiency and ensuring the quality of separation and purification. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0023] Figure 1 This is a front view structural schematic diagram of the utility model;

[0024] Figure 2 It is a three-dimensional structural schematic diagram of the utility model;

[0025] Figure 3 It is a schematic diagram of the three-dimensional cross-sectional structure of the shell of the utility model;

[0026] Figure 4 This is a three-dimensional structural diagram of the adjustment mechanism of the utility model;

[0027] Figure 5 It is a schematic diagram of the three-dimensional structure of the ring plate of the utility model.

[0028] The following are the descriptions of the reference numerals:

[0029] 1. Shell; 2. Support feet; 3. Sealing cover; 4. Ventilation holes; 5. Ring plate; 6. Rotating assembly; 7. Motor; 8. Ring gear; 9. Gear; 10. Electric push rod; 11. Support block; 12. Movable plate; 13. Placement tube; 14. Adjustment mechanism; 15. Support frame; 16. Push rod; 17. Telescopic rod 1; 18. Spring 1; 19. Pulley; 20. Fixed frame; 21. Telescopic rod 2; 22. Spring 2. DETAILED DESCRIPTION

[0030] In order to make the purpose, technical solution and advantages of the utility model clearer, the technical solution of the utility model will be described in detail below. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other implementation methods obtained by ordinary technicians in this field without creative work belong to the scope of protection of the utility model.

[0031] See also Figure 1-Figure 5 As shown, the utility model provides a DNA purification device, including a housing 1, a ring plate 5 is horizontally rotatably arranged in the housing 1, and a rotating assembly 6 is arranged in the housing 1 for rotating the ring plate 5;

[0032] A plurality of placement tubes 13 for placing centrifuge tubes are rotatably arranged on the ring plate 5, and the plurality of placement tubes 13 are circumferentially distributed on the ring plate 5 with the axis of the ring plate 5 as the center;

[0033] An adjusting mechanism 14 is provided in the housing 1 for adjusting the horizontality of the placement tube 13 .

[0034] As an optional embodiment, the shell 1 is a circular cylinder. This shape makes it easier to install the ring plate 5 into the shell 1. The upper side of the shell 1 is threadedly connected with a sealing cover 3, and the threaded connection makes the sealing cover 3 not easy to fall off.

[0035] A plurality of ventilation holes 4 are provided at the bottom of the shell 1 , and a plurality of supporting legs 2 are provided at the bottom of the shell 1 .

[0036] The rotating assembly 6 includes a ring gear 8, which is fixedly arranged at the bottom of the ring plate 5. A motor 7 is arranged in the housing 1, and a gear 9 is fixed on the output shaft of the motor 7. The gear 9 is meshed with the ring gear 8. The motor 7 rotates the gear 9, and the gear 9 cooperates with the ring gear 8 to drive the ring plate 5.

[0037] The center of the ring gear 8 is on the axis of the ring plate 5 .

[0038] The placement tube 13 is rotatably arranged on the upper surface of the ring plate 5 through the support frame 15 .

[0039] The openings of the plurality of placement tubes 13 are opposite to each other.

[0040] The adjusting mechanism 14 includes a plurality of telescopic rods 17, which are vertically arranged on the ring plate 5, and the number of the telescopic rods 17 is consistent with the placement tube 13. The movable end of the telescopic rod 17 is provided with a pulley 19, and the pulley 19 is in contact with the bottom of the placement tube 13. The surface of the telescopic rod 17 is sleeved with a spring 18, and the two ends of the spring 18 are respectively fixed to the movable end and the fixed end of the telescopic rod 17. The movable plate 12 is slidably arranged at the hollow center of the ring plate 5. The upper side of the ring plate 5 is respectively vertically arranged by two fixing frames 20. Two telescopic rods 21 are fixed vertically, and the movable ends of the telescopic rods 21 are fixed to the movable plate 12. The surface of the telescopic rods 21 is sleeved with springs 22, and the two ends of the springs 22 are respectively fixed to the two ends of the telescopic rods 21. The movable plate 12 is provided with push rods 16 in the same number as the placement tubes 13, and the push rods 16 correspond to the bottom of the placement tubes 13. An electric push rod 10 is vertically provided at the bottom of the inner wall of the shell 1, and a support block 11 is rotatably provided at the movable end of the electric push rod 10, and the support block 11 corresponds to the bottom of the movable plate 12;

[0041] The initial state of the placement tube 13 is a horizontal state. During centrifugal purification, the placement tube 13 also needs to be in a horizontal state, which can improve the effect of centrifugal purification.

[0042] When the placement tube 13 needs to be tilted downward, the electric push rod 10 needs to drive the support block 11 to move upward, and the support block 11 needs to push the movable plate 12 upward, and the movable plate 12 needs to drive the top rod 16 to move upward, and the placement tube 13 needs to be pushed upward by the top rod 16 to tilt the placement tube 13. At that time, under the action of gravity, the high-density substances will still all be at the lowest part of the centrifuge tube, and no secondary mixing of the solution will occur.

[0043] With the above structure, the centrifuge tube is placed in the placement tube 13, the posture of the placement tube 13 is adjusted to be horizontal through the adjustment mechanism 14, the sealing cover 3 is placed on the housing 1, and then the ring plate 5 is rotated by the rotating assembly 6, and the placement tube 13 is driven to rotate through the ring plate 5. Under the action of centrifugal force, the material with high density in the centrifuge tube will quickly move to one end of the centrifuge tube;

[0044] Before the ring plate 5 stops rotating, the closed ends of all the placement tubes 13 are tilted downward by the adjusting mechanism 14, so as to avoid the placement tubes 13 remaining in a horizontal state after the centrifugal force is lost, resulting in the remixing of the separated substances in the centrifuge tubes.

[0045] The above is only a specific implementation of the utility model, but the protection scope of the utility model is not limited thereto. Any technician familiar with the technical field can easily think of changes or substitutions within the technical scope disclosed by the utility model, which should be included in the protection scope of the utility model. Therefore, the protection scope of the utility model should be based on the protection scope of the claims.

Claims

1. A DNA purification device, characterized in that: It comprises a housing (1), a ring plate (5) being arranged in a horizontally rotatable manner inside the housing (1), and a rotating assembly (6) being arranged in the housing (1) for rotating the ring plate (5); A plurality of placement tubes (13) for placing centrifuge tubes are rotatably arranged on the ring plate (5), and the plurality of placement tubes (13) are distributed on the ring plate (5) in a circle with the axis of the ring plate (5) as the center; An adjusting mechanism (14) is provided in the housing (1) for adjusting the horizontality of the placement tube (13); The adjusting mechanism (14) comprises a plurality of telescopic rods (17), the telescopic rods (17) being vertically arranged on the ring plate (5), and the number of the telescopic rods (17) being the same as the number of the placement tubes (13), the movable end of the telescopic rod (17) being provided with a pulley (19), and the pulley (19) being in contact with the bottom of the placement tube (13), the surface of the telescopic rod (17) being sleeved with a spring (18), the two ends of the spring (18) being respectively fixed to the movable end and the fixed end of the telescopic rod (17), the central hollow part of the ring plate (5) being slidably provided with a movable plate (12), the upper side of the ring plate (5) being respectively vertically arranged through two fixing frames (20) Two telescopic rods (21) are fixed vertically, and the movable ends of the telescopic rods (21) are fixed to the movable plate (12). The surface of the telescopic rods (21) is sleeved with springs (22), and the two ends of the springs (22) are respectively fixed to the two ends of the telescopic rods (21). The movable plate (12) is provided with push rods (16) whose number is the same as that of the placement tubes (13), and the push rods (16) correspond to the bottom of the placement tubes (13). An electric push rod (10) is vertically provided at the bottom of the inner wall of the shell (1), and a support block (11) is rotatably provided at the movable end of the electric push rod (10), and the support block (11) corresponds to the bottom of the movable plate (12).

2. A DNA purification device according to claim 1, characterized in that: The shell (1) is a circular cylinder, and a sealing cover (3) is threadedly connected to the upper side of the shell (1).

3. A DNA purification device according to claim 1, characterized in that: The bottom of the shell (1) is provided with a plurality of ventilation holes (4), and the bottom of the shell (1) is provided with a plurality of supporting legs (2).

4. A DNA purification device according to claim 1, characterized in that: The rotating assembly (6) comprises a ring gear (8), wherein the ring gear (8) is fixedly arranged at the bottom of the ring plate (5), a motor (7) is arranged in the housing (1), a gear (9) is fixed on the output shaft of the motor (7), and the gear (9) is meshed with the ring gear (8).

5. A DNA purification device according to claim 4, characterized in that: The center of the gear ring (8) is on the axis of the ring plate (5).

6. A DNA purification device according to claim 1, characterized in that: The placement tube (13) is rotatably arranged on the upper surface of the ring plate (5) via a support frame (15).

7. A DNA purification device according to claim 1, characterized in that: The openings of the plurality of placement tubes (13) are opposite to each other.

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