Solation device for nucleic acid agarose gel

Abstract

The utility model provides a nucleic acid agarose gel dissolving device which comprises a heating device and a gel containing device, the gel containing device comprises a cup body and a cup cover, a heat insulation handle is arranged on the side wall of the cup body, and a magnetic stirrer is placed in the cup body; the cup cover is connected with the cup body through a buckle, and a temperature sensing display screen is arranged on the cup cover; the heating device is divided into a function control area and a heating area, the heating area is provided with a heating plate, a heating module at the bottom of the heating plate conducts rapid heating through an iron-chromium-aluminum alloy heating wire, a magnetic core is wrapped in the heating module, and a magnetic field changes when the magnetic core is powered on and changes. The magnetic stirrer rotates to stir the agarose and the TAE solution to be fully mixed. The nucleic acid agarose gel dissolving device is small and light in whole, small in occupied area, easy to move and suitable for being used on a laboratory operation table and a clean bench.

Description

Technical Field

[0001] The utility model relates to a sol device for nucleic acid agarose gel. Background Art

[0002] Agarose gel electrophoresis is a technique based on the molecular sieving effect of agarose gel, which separates charged biomolecules (such as DNA, RNA, and proteins) in gel through the action of electric field. This technique uses gel formed by agarose as a separation medium. Agarose is a polysaccharide with viscosity and gel properties. During the electrophoresis process, the molecules in the gel are affected by the electric field and migrate toward the electrode. In order to visualize the separation results, nucleic acid dyes (such as GoldView) can be added to the gel. After the electrophoresis is completed, the separated band pattern is observed and analyzed by ultraviolet irradiation and development, so as to obtain the relative size and migration distance of the target biomolecules.

[0003] Agarose gel electrophoresis technology has a wide range of applications in molecular biology, including but not limited to DNA analysis, RNA analysis, and protein-nucleic acid interaction research. Agarose gel includes four steps: agarose sol, gel preparation, spotting and electrophoresis, and gel imaging observation. In the agarose sol step, microwave ovens are often used to heat the agarose powder and TAE buffer (or TBE buffer) to fully mix and dissolve. However, microwave ovens occupy a large area, and it is not easy to observe the dissolution of agarose in real time during the heating process. In order to fully dissolve, it is necessary to pause the heating process and continuously shake the glass triangular flask containing agarose and TAE buffer, but the glass triangular flask is hot and difficult to take after heating. As the concentration of agarose gel increases, the heating time will also be prolonged, which will cause the solution to boil and splash. After the agarose is fully dissolved, the solution is in a high-temperature transparent liquid state, but at this time the solution temperature is very high, and the addition of nucleic acid dyes (such as Gold View) is likely to cause its high-temperature degradation, affecting the subsequent agarose gel UV exposure and development observation, so it is necessary to wait until the agarose solution cools down to 50-60°C before adding nucleic acid dyes (such as Gold View). However, the cooling time is related to the ambient temperature and the concentration and volume of the agarose solution, which is not easy to master; and the temperature of the agarose solution can only be judged subjectively by feel, which can easily cause the agarose solution to solidify before gel preparation, thereby causing gel preparation failure. Utility Model Content

[0004] The utility model provides a nucleic acid agarose gel sol device, the main purpose of which is to uniformly heat agarose and TAE solution and fully dissolve them into a clear and transparent liquid state, and the entire heating process can be adjusted, the agarose dissolution process can be visually observed in real time, the temperature of the agarose solution can be observed in real time, and the time for adding nucleic acid dye can be mastered, and the entire device is small and light, occupies a small area, is easy to move, and is suitable for use on laboratory operating tables and ultra-clean workbenches.

[0005] The technical solution adopted by the utility model is:

[0006] A nucleic acid agarose gel sol device, characterized in that it comprises a heating device and a gel containing device, wherein:

[0007] The glue holding device comprises a cup body and a cup cover, the side wall of the cup body is provided with a heat-insulating handle, a magnetic stirrer is placed in the cup body; the cup cover is connected to the cup body through a buckle, and a temperature sensing display screen is provided on the cup cover;

[0008] The heating device is divided into a function control area and a heating area. The heating area is provided with a heating plate. The heating module at the bottom of the heating plate is heated by an iron-chromium-aluminum alloy heating wire. A magnetic core is wrapped in the heating module. When the magnetic core is energized, the magnetic stirrer can rotate.

[0009] The nucleic acid agarose gel sol device, wherein the function control area is provided with a liquid crystal digital display screen and a plurality of sensing buttons.

[0010] The nucleic acid agarose gel sol device, wherein the several sensing buttons include: a start / pause button, a temperature adjustment button, a timing button and a speed adjustment button.

[0011] The nucleic acid agarose gel sol device, wherein the heating device is connected to a plug via a power cord, and the power cord is also provided with a power switch to control the on / off of the heating device.

[0012] The nucleic acid agarose gel sol device, wherein the outer dimensions of the heating device are 22 cm long × 16 cm wide × 7.5 cm high, the outer dimensions of the heating zone are 16 cm long × 16 cm wide × 7.5 cm high, the heating plate is circular, and its outer dimensions are 13 cm in diameter × 3 cm in height; the outer dimensions of the function control zone are 16 cm long × 6 cm wide × 4.5 cm high.

[0013] The nucleic acid agarose gel sol device, wherein the outer dimensions of the liquid crystal digital display screen are 13 cm in length and 2 cm in width.

[0014] In the nucleic acid agarose gel sol device, the heating module at the bottom of the heating plate is an iron-chromium-aluminum alloy heating wire, and a mica soft plate is used as an insulating material.

[0015] The nucleic acid agarose gel sol device, wherein the cup body of the gel holding device is made of stainless steel, and the heat-insulating handle is made of stainless steel coated with polyether ketone (PEEK).

[0016] The nucleic acid agarose gel sol device, wherein the cup body has scales inside.

[0017] The nucleic acid agarose gel sol device, wherein the cup cover is made of transparent high temperature resistant plastic material.

[0018] The beneficial effects of the utility model are:

[0019] (1) The nucleic acid agarose gel sol device is small and light overall, occupies a small area, is easy to move, and is suitable for use on laboratory operating tables and clean workbenches.

[0020] (2) The heating process is controllable. The heating device can adjust the temperature, time and speed. During the heating process, the magnetic stirrer rotates to ensure that the agarose and TAE solution are fully mixed and dissolved, and at the same time, the liquid is heated evenly to prevent local uneven heating from causing incomplete dissolution of agarose.

[0021] (3) The cup of the gel container has good thermal conductivity, which makes it easy to quickly dissolve agarose and TAE solution. It has a heat-insulating handle that makes it easy to take and pour the solution. The transparent cup cover makes it easy to observe the dissolution of agarose in real time, and can display the temperature in real time, making it easy to grasp the time to add nucleic acid dyes (such as Gold View); it can also prevent water evaporation during the heating process. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 , Figure 2 They are respectively a top view and a three-dimensional view of the heating device.

[0023] Figure 3 , Figure 4 They are respectively a top view of the cup cover of the glue holding device and a front view of the cup body.

[0024] Explanation of the accompanying drawings: 1-function control area; 2-heating area; 3-heating plate; 4-power switch; 5-power cord; 6-plug; 7-LCD digital display screen; 8-start / pause button; 9-temperature adjustment button; 10-timing button; 11-speed adjustment button; 12-cup body; 13-cup lid; 14-insulated handle; 15-buckle; 16-temperature sensing display screen; 17-magnetic stirring bar. DETAILED DESCRIPTION

[0025] like Figure 1-Figure 4 As shown, the utility model provides a nucleic acid agarose gel sol device, including a heating device and a gel containing device, wherein:

[0026] The heating device is divided into a function control area 1 and a heating area 2. The function control area 1 is provided with a liquid crystal digital display screen 7 and four induction buttons (i.e., a start / pause button 8, a temperature adjustment button 9, a timing button 10, and a speed adjustment button 11), and is connected to a plug 6 through a power cord 5, and a power switch button 4 is provided to control the power on and off; a circular heating plate 3 is provided on the heating area 2;

[0027] Wherein, the power cord 5 and the plug 6 are used to supply power to the heating device, the power switch 4 controls the opening and closing of the heating device, the start / pause key 8 controls the start and pause of the circular heating plate 3, the temperature adjustment key 9 is used to set the working temperature of the circular heating plate 3, and the temperature adjustment range is room temperature-300°C, the timing key 10 is used to set the working time of the circular heating plate 3, and the time adjustment range is 1min (minutes)-24h (hours), and the speed adjustment key 11 is used to set the speed of the magnetic stirrer 17 (described in detail later), and the speed adjustment range is 0rpm (speed)-3000rpm (speed);

[0028] Among them, each induction button of the above-mentioned heating device realizes the above-mentioned control setting function through the internal circuit of the heating device, and the internal circuit (such as a stable control circuit, a timing circuit, etc.) is a conventional technical means in the prior art, and its specific implementation method is omitted here;

[0029] The glue holding device comprises a cup body 12 and a cup cover 13. The side wall of the cup body 12 is provided with a heat-insulating handle 14. A magnetic stirrer 17 is placed in the cup body 12. The cup cover 13 is connected to the cup body 12 via a buckle 15. The cup cover 13 is provided with a temperature sensing display screen 16.

[0030] The dimensions of the heating device are 22 (length) × 16 (width) × 7.5 (height) cm, the dimensions of the heating zone 2 are 16 (length) × 16 (width) × 7.5 (height) cm, and the dimensions of the circular heating plate 3 are 13 (diameter) × 3 (height) cm. The dimensions of the function control zone 1 are 16 (length) × 6 (width) × 4.5 (height) cm, and the dimensions of the liquid crystal digital display screen 7 are 13 (length) × 2 (width) cm.

[0031] Among them, the heating module at the bottom of the heating plate 3 uses an iron-chromium-aluminum alloy heating wire for rapid heating, and uses a mica soft board as an insulating material; a magnetic core is wrapped in the heating module at the bottom of the heating plate 3, and a magnetic field change will be generated when a changing current is passed, so that the magnetic stirrer 17 rotates and stirs the liquid to mix, while achieving the effect of uniformly heating the liquid; the outer shell of the heating plate 3 is made of stainless steel and has good thermal conductivity.

[0032] Among them, the cup body 12 of the glue holding device is made of stainless steel with good thermal conductivity; the insulating handle 14 on the side wall of the cup body 12 is made of stainless steel with an insulating material polyetherketone (PEEK) applied on the outside. PEEK has the advantages of low thermal conductivity, heat insulation and high temperature resistance, and plays a good insulating role, making it easy to take the cup body 12; there is a scale inside the cup body 12, which is easy to measure the volume of the solution.

[0033] Among them, the cup cover 13 is made of transparent high-temperature resistant plastic material, which is easy to observe the agarose dissolution process in real time and can also prevent water evaporation; at the same time, there is a temperature sensing display screen 16 on the cup cover. After touching the display screen, the temperature of the agarose solution in the cup body 12 detected by the temperature sensor can be displayed in real time, which makes it easy to grasp the time of adding nucleic acid dye (such as Gold View), which will significantly improve the efficiency of making agarose gel.

[0034] The operation process of the utility model is as follows:

[0035] (1) Prepare agarose gel of a certain concentration according to experimental needs, generally 1%-2% concentration ratio. For example, to prepare 100ml of 1% agarose gel, weigh 1g of agarose powder and put it into the cup 12 of the gel holding device, then add 100ml of 1×TAE (or TBE) buffer, and put the magnetic stirrer 17 into the cup 12.

[0036] (2) Plug the plug 6 into the external power socket (220V), turn on the power switch 4, adjust the temperature adjustment key 9 to 100°C, adjust the timer key 10 to 5min, adjust the speed adjustment key 11 to 200rpm, and turn on the start / pause key 8.

[0037] (3) During the heating process, observe the dissolution process of agarose through the cup cover 13 until the solution becomes clear and transparent, turn off the start / pause key 8, turn off the power switch key 4, and unplug the power plug 6.

[0038] (4) The temperature of the agarose solution in the cup body 11 is observed in real time through the temperature sensing display screen 15 on the cup cover 13. When the temperature of the solution drops to 50-60°C, 5ul of nucleic acid dye (such as Gold View) is added to the solution and mixed. Then, the second step, i.e., the agarose gel preparation step, is performed. Holding the heat-insulating handle 14, the agarose solution with the nucleic acid dye (such as Gold View) added is poured into the gel preparation rack to prepare the agarose gel.

[0039] (5) After about 10-20 minutes, when the agarose solution solidifies into a colloidal solid state, the third step, namely spotting and electrophoresis, is carried out. The electrophoresis is carried out for about 20-50 minutes (the electrophoresis time is set in combination with the target band size of the experimental sample and the concentration of the agarose gel), and then the electrophoresis is stopped and the electrophoresis instrument is turned off. The fourth step is to place the agarose gel into a gel imaging system for ultraviolet exposure and development observation.

Claims

1. A nucleic acid agarose gel sol device, characterized in that: It includes a heating device and a glue holding device, wherein: The glue holding device comprises a cup body and a cup cover, the side wall of the cup body is provided with a heat-insulating handle, a magnetic stirrer is placed in the cup body; the cup cover is connected to the cup body through a buckle, and a temperature sensing display screen is provided on the cup cover; The heating device is divided into a function control area and a heating area. The heating area is provided with a heating plate. The heating module at the bottom of the heating plate is heated by an iron-chromium-aluminum alloy heating wire. A magnetic core is wrapped in the heating module. When the magnetic core is energized, the magnetic stirrer can rotate.

2. The nucleic acid agarose gel sol device according to claim 1, characterized in that: The function control area is provided with a liquid crystal digital display screen and a plurality of induction buttons.

3. The nucleic acid agarose gel sol device according to claim 2, characterized in that: The plurality of sensing buttons include: a start / pause button, a temperature adjustment button, a timing button and a speed adjustment button.

4. The nucleic acid agarose gel sol device according to claim 2, characterized in that: The heating device is connected to a plug via a power line, and the power line is also provided with a power switch to control the opening and closing of the heating device.

5. The nucleic acid agarose gel sol device according to claim 1, characterized in that: The dimensions of the heating device are 22 cm long × 16 cm wide × 7.5 cm high, the dimensions of the heating zone are 16 cm long × 16 cm wide × 7.5 cm high, the heating plate is circular, and its dimensions are 13 cm in diameter × 3 cm in height; the dimensions of the function control zone are 16 cm long × 6 cm wide × 4.5 cm high.

6. The nucleic acid agarose gel sol device according to claim 2, characterized in that: The dimensions of the liquid crystal digital display screen are 13 cm in length and 2 cm in width.

7. The nucleic acid agarose gel sol device according to claim 1, characterized in that: The heating module at the bottom of the heating plate is an iron-chromium-aluminum alloy heating wire, and a mica soft plate is used as an insulating material.

8. The nucleic acid agarose gel sol device according to claim 1, characterized in that: The cup body of the glue holding device is made of stainless steel, and the heat-insulating handle is made of stainless steel coated with PEEK.

9. The nucleic acid agarose gel sol device according to claim 1, characterized in that: The cup body has scales inside.

10. The nucleic acid agarose gel sol device according to claim 1, characterized in that: The cup cover is made of transparent high-temperature resistant plastic material.

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