Injection type multi-grid antibody incubation box

Abstract

The utility model discloses an injection type multi-cell antibody incubation box which comprises an antibody incubation box body, a top cover and a partition plate, the top of the antibody incubation box body is in sliding connection with the bottom of the top cover, and the bottom of the inner wall of the antibody incubation box body is fixedly connected with the bottom of the partition plate. The top of the left side of the inner wall of the antibody incubation box body is fixedly connected with a discharging pipe, and the bottom of the discharging pipe is fixedly connected with a discharging head. According to the antibody incubation box, the water pump is arranged, the water pump is started to conduct antibody diluent of an antibody liquid source to the discharging pipe and inject the antibody diluent into the antibody incubation box body through the discharging head, and the problems that due to the lack of an auxiliary liquid adding function, manual liquid adding is needed when a user uses the antibody incubation box, the antibody incubation efficiency is reduced, and meanwhile dust in air is prone to being attached to a cover; the problem that dust easily falls into the antibody incubation box to cause pollution in the process of opening the cover is solved, so that the experiment accuracy is reduced, and the effects of automatically replenishing liquid and cleaning are achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of medical experimental devices, and particularly relates to an injection-type multi-compartment antibody incubation box. Background Technique

[0002] Immunoblotting assay, also known as protein blotting method, Western Blot, is a commonly used experimental method in molecular biology. Its basic principle is to stain the cell or biological tissue samples processed by gel electrophoresis with specific antibodies, and obtain information on the expression of specific proteins in the analyzed cells or tissues by analyzing the staining position and depth, that is, separating the target protein by electrophoresis and transferring it to a PVDF membrane, then hybridizing with a known protein antibody, and finally qualitatively and quantitatively analyzing the target protein through the color reaction of the enzyme system. It mainly includes 5 steps: electrophoresis, transfer, blocking, hybridization and color development, and the blocking and hybridization processes are also called antibody incubation processes.

[0003] For example, application number: CN202222442163.8, the utility model discloses an antibody incubation box, which includes a box body and a box cover. An incubation groove is installed in the box body. There are more than two chambers in the incubation groove. There are slot holes at the bottom of the chamber. A lifting component is arranged in the cavity between the lower surface of the incubation groove and the bottom surface of the box body. A sealing plate covering the slot holes is arranged at the top of the lifting component. When the incubation groove is stressed, it can move down along the inner wall of the box body, so that the sealing plate moves away from the slot holes and the slot holes are opened. When in use, the sealing plate at the top of the lifting component covers the slot holes, pour the primary antibody slow-release liquid for incubation. After the incubation is completed, apply force to press down the incubation groove to make it move down, the sealing plate disengages from the slot holes to open the slot holes, the slow-release liquid flows out from the slot holes to recover the antibody, and then the incubation groove is reset through the lifting component, and the sealing plate covers the slot holes again. In this way, the antibody incubation process can be realized without taking out the PVDF membrane, the operation is more convenient, and the pollution risk brought by the external environment is reduced.

[0004] Based on the retrieval of the above patent and the combination with the equipment in the prior art, it is found that when the above equipment is applied, although it can solve the problems of repeatedly taking out and putting in the PVDF membrane and pouring the liquid in the box, the process is not only repeated and complicated, but also increases the pollution risk of the external environment to the PVDF membrane. However, during the use process, it lacks the function of auxiliary liquid replenishment, resulting in the need for manual liquid replenishment by the user during use, reducing the antibody incubation efficiency. At the same time, the lid is easy to adhere to dust in the air, resulting in the situation that dust is easily fallen into the antibody incubation box during the process of opening the lid, thus reducing the experimental accuracy. Content of the Utility Model

[0005] To solve the problems raised in the above-mentioned background art, the purpose of the present utility model is to provide an injection-type multi-compartment antibody incubation box, which has the advantages of automatic liquid replenishment and cleaning, solves the problem of lacking the function of auxiliary liquid replenishment, resulting in the need for manual liquid replenishment during use by the user, reducing the antibody incubation efficiency. At the same time, the cover is prone to adhering to dust in the air, resulting in the situation that dust is easily fallen into the antibody incubation box during the process of opening the cover, causing pollution, thereby reducing the problem of experimental accuracy.

[0006] To achieve the above purpose, the present utility model provides the following technical solutions: An injection-type multi-compartment antibody incubation box includes an antibody incubation box body, a top cover, and a partition plate. The top of the antibody incubation box body is slidably connected to the bottom of the top cover. The bottom of the inner wall of the antibody incubation box body is fixedly connected to the bottom of the partition plate. The top of the left side of the inner wall of the antibody incubation box body is fixedly connected with a discharge pipe. The bottom of the discharge pipe is fixedly connected with a discharge head. There are several discharge heads and they are distributed at equal distances. The top of the right side of the antibody incubation box body is fixedly connected with a water pump. The bottom of the water pump is fixedly connected with a support frame. The output end of the water pump is communicated with the discharge pipe. The input end of the water pump is communicated with an antibody liquid source through a conduit. The right side of the top of the top cover is movably connected with a cleaning component. Activity mechanisms are provided at the top of both sides of the antibody incubation box body. The front side of the right side of the antibody incubation box body is fixedly connected with a time relay. The time relay is electrically connected to the water pump through a wire.

[0007] As a preference of the present utility model, the cleaning component includes a scraping strip. The bottom of the scraping strip is in contact with the top of the right side of the top cover. A transmission plate is arranged at the top of the scraping strip.

[0008] As a preference of the present utility model, the activity mechanism includes an activity groove. The activity groove is opened at the top of both sides of the antibody incubation box body. Sliding members are fixedly connected to both sides of the transmission plate. The bottom of the inner side of the sliding member is slidably connected to the inner wall of the activity groove. The top of the left side of the antibody incubation box body is fixedly connected with a limiting plate.

[0009] As a preference of the present utility model, a plug rod is fixedly connected to the top of the scraping strip. The top of the plug rod penetrates through the top of the transmission plate. A stud is threadedly connected to the top of the plug rod.

[0010] As a preference of the present utility model, a spring is sleeved on the surface of the stud. One end of the spring is fixedly connected to the top of the transmission plate.

[0011] As a preference of the present utility model, anti-slip grooves are opened at the top of the surface of the stud. There are several anti-slip grooves and they are distributed at equal distances.

[0012] Preferably, four corners of the bottom of the antibody incubation box body are provided with buffer support pads, and the buffer support pads are fixedly connected with the antibody incubation box body through PET glue.

[0013] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0014] 1. By setting a water pump, when the water pump is turned on, the antibody diluent of the anti-liquid source is conducted to the discharge pipe and injected into the interior of the antibody incubation box body through the discharge head, solving the problem that the lack of the function of auxiliary liquid replenishment leads to the need for manual liquid replenishment by the user during use, reducing the antibody incubation efficiency, and at the same time, the cover is easily adhered with dust in the air, resulting in the situation that dust is easily fallen into the antibody incubation box during the process of opening the cover, thus reducing the experimental accuracy, and achieving the effect of automatic liquid replenishment and cleaning.

[0015] 2. By setting a cleaning component, when the user needs to open the top cover, the user can first push the transmission plate to move to the left, so that the transmission plate drives the corresponding scraping strip to move to the left, and then the scraping strip will scrape and clean the dust accumulated on the top cover. At this time, the user can open the top cover to avoid dust falling into the interior of the antibody incubation box body and reducing the possibility of contamination.

[0016] 3. By setting a moving mechanism, during the movement of the transmission plate, the sliding fit of the moving groove and the sliding part can be used to limit the movement track of the transmission plate to prevent deviation. After the transmission plate moves to the leftmost side, the user can drive the sliding part to rotate counterclockwise by half and place it on the limiting plate to ensure that it does not affect the opening of the top cover. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is a three-dimensional structural schematic diagram of the present utility model;

[0018] Figure 2 is a three-dimensional structural schematic diagram of the antibody incubation box body of the present utility model;

[0019] Figure 3 is a partial component explosion schematic diagram of the transmission plate of the present utility model.

[0020] In the figure: 1. Antibody incubation box body; 2. Top cover; 3. Partition board; 4. Discharge pipe; 5. Discharge head; 6. Water pump; 7. Support frame; 8. Cleaning component; 81. Scraping strip; 82. Transmission plate; 9. Moving mechanism; 91. Moving groove; 92. Sliding part; 93. Limiting plate; 10. Time relay; 11. Insertion rod; 12. Stud; 13. Spring; 14. Anti-slip groove; 15. Buffer support pad. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0021] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the protection scope of the present utility model.

[0022] As Figures 1 to 3 shown, an injection multi-compartment antibody incubation box provided by the present utility model includes an antibody incubation box body 1, a top cover 2 and a partition plate 3. The top of the antibody incubation box body 1 is slidably connected to the bottom of the top cover 2. The bottom of the inner wall of the antibody incubation box body 1 is fixedly connected to the bottom of the partition plate 3. The top of the left side of the inner wall of the antibody incubation box body 1 is fixedly connected with a discharge pipe 4. The bottom of the discharge pipe 4 is fixedly connected with a discharge head 5. A plurality of discharge heads 5 are provided and are distributed at equal intervals. The top of the right side of the antibody incubation box body 1 is fixedly connected with a water pump 6. The bottom of the water pump 6 is fixedly connected with a support frame 7. The output end of the water pump 6 is communicated with the discharge pipe 4. The input end of the water pump 6 is communicated with an antibody liquid source through a conduit. The right side of the top of the top cover 2 is movably connected with a cleaning component 8. Activity mechanisms 9 are provided at the top of both sides of the antibody incubation box body 1. The front side of the right side of the antibody incubation box body 1 is fixedly connected with a time relay 10. The time relay 10 is electrically connected to the water pump 6 through a wire.

[0023] Referring Figure 1 to

[0024] As a technical optimization solution of the present utility model, by providing the cleaning component 8, when the user needs to open the top cover 2, the user can first push the transmission plate 82 to move to the left, so that the transmission plate 82 drives the corresponding scraping strip 81 to move to the left. Then the scraping strip 81 will scrape and clean the dust accumulated on the top cover 2. At this time, the user can open the top cover 2 to avoid dust falling into the interior of the antibody incubation box body 1 and reduce the possibility of contamination.

[0025] Referring Figure 1 to

[0026] As a technical optimization solution of the present utility model, by providing a movable mechanism 9, during the movement of the transmission plate 82, the sliding fit between the movable groove 91 and the sliding member 92 can be utilized to limit the movement trajectory of the transmission plate 82 and prevent deviation. After the transmission plate 82 moves to the leftmost side, the user can drive the sliding member 92 to rotate counterclockwise by half and place it on the limiting plate 93 to ensure that it does not affect the opening of the top cover 2.

[0027] Reference Figure 3 , a plug rod 11 is fixedly connected to the top of the scraping strip 81. The top of the plug rod 11 penetrates through the top of the transmission plate 82, and a stud 12 is threadedly connected to the top of the plug rod 11.

[0028] As a technical optimization solution of the present utility model, by providing the plug rod 11 and the stud 12, when the scraping strip 81 needs to be replaced due to aging after long-term use by the user, the user can rotate and loosen the stud 12 to remove it, and then the user can disconnect the plug rod 11 from the transmission plate 82, and then install a new scraping strip 81 and threadedly connect it with the plug rod 11 on the new scraping strip 81 using a bolt.

[0029] Reference Figure 3 , a spring 13 is sleeved on the surface of the stud 12, and one end of the spring 13 is fixedly connected to the top of the transmission plate 82.

[0030] As a technical optimization solution of the present utility model, by providing the spring 13, the elastic force of the spring 13 can be utilized to push the scraping strip 81, so that the bottom of the scraping strip 81 fits more closely to the top of the top cover 2, improving the cleaning effect of the scraping strip 81 on the top of the top cover 2 and avoiding the situation of poor cleaning effect.

[0031] Reference Figure 3 , anti-slip grooves 14 are formed in the top of the surface of the stud 12, and a plurality of anti-slip grooves 14 are formed and are equally spaced.

[0032] As a technical optimization solution of the present utility model, by providing the anti-slip grooves 14, when the user needs to rotate the stud 12, the user's hand can contact the stud 12 through the anti-slip grooves 14, thus greatly improving the friction between the user's hand and the stud 12 and preventing slipping.

[0033] Reference Figure 1 , buffer support pads 15 are provided at the four corners of the bottom of the antibody incubation box body 1, and the buffer support pads 15 are fixedly connected to the antibody incubation box body 1 through PET glue.

[0034] As a technical optimization solution of the present utility model, by providing a buffer support pad 15, the bottom of the antibody incubation box body 1 contacts the ground through the buffer support pad 15, avoiding the violent vibration between the antibody incubation box body 1 and the ground driven by the vibration generated when the water pump 6 is turned on, improving the use stability of the antibody incubation box body 1. Then, by utilizing the advantage of strong adhesiveness of PET glue, the fixing strength of the buffer support pad 15 is improved.

[0035] The working principle and usage process of the present utility model: When using the antibody incubation box body 1, the user connects the input end of the water pump 6 to the antibody solution source, and then starts the time relay 10 for the first timing. When the timing of the time relay 10 ends, an electrical signal will be sent to turn on the water pump 6 to conduct the antibody diluent from the antibody solution source to the discharge pipe 4 and inject it into the interior of the antibody incubation box body 1 through the discharge head 5. At the same time, the time relay 10 conducts the second timing. When the timing ends, an electrical signal will be sent to turn off the water pump 6 and start the first timing again, and so on in a cycle. When the user needs to open the top cover 2, the user can first push the transmission plate 82 to move to the left, so that the transmission plate 82 drives the corresponding scraping strip 81 to move to the left. Then the scraping strip 81 will scrape and clean the dust accumulated on the top cover 2. At this time, the user can open the top cover 2 to avoid dust falling into the interior of the antibody incubation box body 1, reducing the possibility of contamination. During the movement of the transmission plate 82, the sliding fit between the movable groove 91 and the sliding member 92 can be used to limit the movement track of the transmission plate 82 to prevent deviation. After the transmission plate 82 moves to the leftmost side, the user can drive the sliding member 92 to rotate counterclockwise by half a turn with the transmission plate 82 and place it on the limiting plate 93 to ensure that it does not affect the opening of the top cover 2, thus having the ability of automatic liquid replenishment and cleaning.

[0036] In summary: For this injection multi-grid antibody incubation box, by providing a water pump 6, turning on the water pump 6 to conduct the antibody diluent from the antibody solution source to the discharge pipe 4 and injecting it into the interior of the antibody incubation box body 1 through the discharge head 5, it solves the problem that the lack of an auxiliary liquid replenishment function requires manual liquid replenishment by the user during use, reducing the antibody incubation efficiency. At the same time, dust in the air is easily adhered to the cover, resulting in dust falling into the antibody incubation box during the opening process, causing contamination, thereby reducing the experimental accuracy.

[0037] It should be noted that in this text, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprising", "including" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or elements inherent to such process, method, article or device.

[0038] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An injection-type multi-compartment antibody incubation box, comprising an antibody incubation box body (1), a top cover (2) and a partition plate (3), characterized in that: The top of the antibody incubation box body (1) is slidably connected to the bottom of the top cover (2); the bottom of the inner wall of the antibody incubation box body (1) is fixedly connected to the bottom of the partition plate (3); a discharge pipe (4) is fixedly connected to the top of the left side of the inner wall of the antibody incubation box body (1); a discharge head (5) is fixedly connected to the bottom of the discharge pipe (4); a plurality of discharge heads (5) are provided and are distributed at equal distances; a water pump (6) is fixedly connected to the top of the right side of the antibody incubation box body (1); the water pump (6) The bottom of the antibody incubation box body (1) is fixedly connected to a support frame (7), the output end of the water pump (6) is connected to the discharge pipe (4), the input end of the water pump (6) is connected to the antibody liquid source through a conduit, the right side of the top of the top cover (2) is movably connected to a cleaning component (8), the tops of both sides of the antibody incubation box body (1) are provided with movable mechanisms (9), the front side of the right side of the antibody incubation box body (1) is fixedly connected to a time relay (10), and the time relay (10) is electrically connected to the water pump (6) through a wire.

2. The injectable multi-compartment antibody incubation box according to claim 1, characterized in that: The cleaning assembly (8) comprises a scraper bar (81), the bottom of the scraper bar (81) contacts the right side of the top of the top cover (2), and a transmission plate (82) is provided on the top of the scraper bar (81).

3. The injectable multi-compartment antibody incubation box according to claim 2, characterized in that: The movable mechanism (9) comprises a movable groove (91), wherein the movable groove (91) is provided at the top of both sides of the antibody incubation box body (1), and both sides of the transmission plate (82) are fixedly connected with sliding members (92), and the bottom of the inner side of the sliding member (92) is slidably connected to the inner wall of the movable groove (91), and the top of the left side of the antibody incubation box body (1) is fixedly connected with a limiting plate (93).

4. The injectable multi-compartment antibody incubation box according to claim 2, characterized in that: The top of the scraper strip (81) is fixedly connected to an insertion rod (11), the top of the insertion rod (11) penetrates the top of the transmission plate (82), and the top of the insertion rod (11) is threadedly connected to a stud (12).

5. The injectable multi-compartment antibody incubation box according to claim 4, characterized in that: A spring (13) is sleeved on the surface of the stud (12), and one end of the spring (13) is fixedly connected to the top of the transmission plate (82).

6. The injectable multi-compartment antibody incubation box according to claim 4, characterized in that: An anti-skid groove (14) is provided at the top of the surface of the stud (12), and a plurality of anti-skid grooves (14) are provided and are distributed at equal distances.

7. The injectable multi-compartment antibody incubation box according to claim 1, characterized in that: Buffer support pads (15) are provided at the four corners of the bottom of the antibody incubation box body (1), and the buffer support pads (15) are fixedly connected to the antibody incubation box body (1) by PET glue.

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