Cell culture dish

Abstract

The utility model discloses a cell culture dish which comprises an elastic ring, and the inner hole wall of the elastic ring is in contact with the side wall of bone matrix gelatin and is used for coating the bone matrix gelatin. The base contacts with the lower end face of the elastic ring and is used for supporting the elastic ring. The end part of the pressing tube is in contact with the upper end surface of the elastic ring, and the interior is used for accommodating cell culture fluid and seed cells. The pressure assembly is connected with the base and the pressure pipe and used for enabling the base and the pressure pipe to be close to each other, enabling the pressure pipe and the base to extrude the elastic ring, enabling the elastic ring to deform, enabling the aperture of an inner hole to be reduced, enabling the inner hole wall of the elastic ring and the side wall of the bone matrix gelatin to extrude each other, and enabling the inner hole wall of the elastic ring to seal a gap between the side wall of the bone matrix gelatin.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture dishes, in particular to a cell culture dish. Background Art

[0002] A petri dish is a laboratory vessel used for microbial or cell culture, usually made of glass or plastic. There are basically two types of petri dish materials, mainly plastic and glass. Glass can be used for plant materials, microbial culture and animal cell adherent culture. Plastic may be made of polyethylene, which can be disposable or reusable. It is suitable for laboratory inoculation, streaking, and bacterial isolation operations, and can be used for the culture of plant materials.

[0003] Bone Matrix Gelatin (BMG) is a scaffold material obtained by removing minerals and cells from bones using chemical and physical methods. BMG is a porous biomaterial similar to a sponge. Cells can be planted, migrate, proliferate and differentiate on the surface and inside of BMG. At the same time, the nutrients required for cell culture and the macromolecules secreted by the cells themselves can also move freely inside BMG. More importantly, BMG has a wide range of sources, has no or very low antigenicity, is non-immunogenic, and can be naturally absorbed in the body without surgical removal. Using BMG as a biological scaffold, tissue-engineered articular cartilage has been successfully constructed in vitro, and the tissue-engineered cartilage constructed in vitro has been transplanted into the body to repair articular cartilage defects. In addition, a large amount of clinical experimental evidence suggests that BMG can be effectively used in the treatment of bone defects.

[0004] Chinese patent publication number: CN 956456102 A discloses a method for constructing epiphyseal cartilage in vitro, including: 1) culturing human chondrocyte cell line or human bone marrow stem cell to form a cell monolayer, digesting with trypsin, washing, resuspending to obtain a cell suspension for counting and standby; 2) cutting the pre-treated bone matrix gelatin material into a cylinder smaller than the diameter of the culture hole, and drilling a hole through the two bottom surfaces of the cylinder on the cylinder, sterilizing; suspending and fixing the cut bone matrix gelatin material in the culture hole; 3) implanting the cell suspension obtained in step 1) through the hole below the bone matrix gelatin material, and making the liquid surface infiltrate the bone matrix gelatin material, and culturing the epiphyseal cartilage. This method establishes a culture system of C28 / I2 cells and a method for constructing epiphyseal cartilage in vitro with it, solving the problem of difficulty in the source of seed cells; the cell properties are stable and the culture cost is low. Preferably, the method for constructing epiphyseal cartilage in vitro as described above, in step 3), before implanting the cell suspension, further comprises: sealing the gap between the bone matrix gelatin material and the edge of the culture well with sterile agarose gel. Sealing the gap between the bone matrix gelatin material and the edge of the culture well can prevent the cells from crawling out of the culture well during growth, and only grow under the bone matrix gelatin material.

[0005] For the above-mentioned related art, when using the culture method, it is necessary to seal the gap between the bone matrix gelatin and the culture hole. However, by the method of sterile agarose gel sealing, when filling the gap between the bone matrix gelatin material and the edge of the culture hole, since the gap has a certain depth, it takes a lot of time to fill the gap, which reduces the efficiency of cell culture. At the same time, during the cell culture process, the gel is immersed in the cell culture fluid for a long time, and it is easy to break away from the gap. During the cell growth process, the cells climb out of the culture hole and grow under the bone matrix gelatin material, which reduces the success rate of cell culture. Utility Model Content

[0006] In view of the above technical problems, the utility model provides a cell culture dish, comprising:

[0007] The elastic ring has an inner hole wall in contact with the side wall of the bone matrix gelatin and is used for coating the bone matrix gelatin.

[0008] The base is in contact with the lower end surface of the elastic ring and is used for supporting the elastic ring.

[0009] The end of the compression tube contacts the upper end surface of the elastic ring, and the interior is used to contain cell culture fluid and seed cells.

[0010] The pressure assembly is connected to the base and the pressure tube, and is used to bring the base and the pressure tube closer to each other, so that the pressure tube and the base squeeze the elastic ring, and the elastic ring deforms to reduce the inner hole diameter, so that the inner hole wall of the elastic ring and the side wall of the bone matrix gelatin squeeze each other, and the gap between the inner hole wall of the elastic ring and the side wall of the bone matrix gelatin is sealed.

[0011] In order to achieve the above object, the utility model is realized by the following technical scheme: when performing cell culture, the bone matrix gelatin is trimmed into a cylindrical shape, the diameter of the trimmed cylindrical bone matrix gelatin is approximately equal to the diameter of the inner hole of the elastic ring, the trimmed bone matrix gelatin is inserted into the elastic ring, the outer wall of the bone matrix gelatin is in contact with the inner hole wall of the elastic ring, the elastic ring is then placed on the base, the lower end face of the elastic ring is in contact with the base, the pressure tube is placed on the upper end of the elastic ring, the base and the pressure tube are brought close to each other through the pressure assembly, so that the pressure tube and the base squeeze the elastic ring, the elastic ring is deformed and the inner hole diameter is reduced, the inner hole wall of the elastic ring and the side wall of the bone matrix gelatin are squeezed each other, the gap between the inner hole wall of the elastic ring and the side wall of the bone matrix gelatin is sealed, and finally the cell culture fluid and seed cells are injected into the pressure tube to perform cell culture. The pressure assembly can use a counterweight block, the counterweight block is fixed to the pressure tube, and pressure is applied to the elastic ring by gravity, so that the elastic ring is deformed. The pressure assembly may also require an elastic member, which is respectively connected to the pressure tube and the base, and applies pressure to the elastic ring through the elastic force of the elastic member.

[0012] Compared with the prior art, the utility model has the following advantages: during cell culture, only the elastic member with built-in bone matrix gelatin needs to be placed on the base, and the edge gap of the bone matrix gelatin can be sealed by compression of the pressure tube and the base, thereby improving the efficiency of cell culture. The edge gap of the bone matrix gelatin is sealed by deformation of the elastic ring, and the inner wall of the elastic ring can be integrally fitted with the outer wall of the bone matrix gelatin, thereby achieving overall sealing of the bone matrix gelatin in the height direction, and the sealing property will not weaken with the change of the cell culture time, so it is difficult for the cells to crawl out of the culture hole during the growth process, and they will not grow under the bone matrix gelatin material, thereby improving the success rate of the culture.

[0013] More preferably, it also includes:

[0014] The piston hole is arranged inside the base and is communicated with the pressure tube.

[0015] The piston is slidably sleeved with the piston hole and is used to form negative pressure between the piston in the piston hole and the bone matrix gelatin.

[0016] By adopting the above technical solution, after adding cell culture fluid and seed cells into the pressure tube, the piston is pushed to move away from the bone matrix gelatin, and negative pressure is formed between the piston and the bone matrix gelatin inside the piston hole. The cell culture fluid and seed cells are sucked into the pores of the bone matrix gelatin, so that the seed cells quickly adhere to the bone matrix gelatin scaffold and grow, further improving the efficiency of cell culture.

[0017] Further preferably, the pressure component comprises:

[0018] The first thread is arranged on the base.

[0019] The second thread is arranged on the pressing tube and matched with the first thread.

[0020] By adopting the above technical solution, the base and the pressing tube are rotated relative to each other. Under the action of the first thread and the second thread, the base and the pressing tube are moved closer to each other to compress the elastic ring. The thread engagement can slowly increase the pressure on the elastic member, causing the elastic ring to slowly deform, thereby avoiding excessive pressure of the elastic ring on the bone matrix gelatin scaffold and preventing the bone matrix gelatin from rupture.

[0021] More preferably, the base is divided into:

[0022] The upper supporting ring contacts the lower end surface of the elastic ring and is used for supporting the elastic ring.

[0023] The lower support seat is in contact with the end of the upper support ring away from the elastic ring. The first thread is arranged on the lower support seat, and the piston hole is arranged in the lower support seat.

[0024] By adopting the above technical solution, the lower support seat is rotated, the lower support seat and the upper support ring rotate relative to each other, the lower support seat and the pressure tube rotate relative to each other, the lower support seat pushes the upper support ring and the pressure tube closer to each other, thereby compressing the elastic ring, and the upper support ring and the pressure tube do not rotate relative to each other, thereby avoiding wear on the elastic ring and increasing the service life of the elastic ring.

[0025] More preferably, it also includes:

[0026] The sealing ring is arranged between the upper supporting ring and the lower supporting seat, and is used for sealing the contact position between the upper supporting ring and the lower supporting seat.

[0027] With the above technical solution, the sealing ring is used to seal the contact position between the upper support ring and the lower support seat to avoid air leakage when the piston moves.

[0028] More preferably, it also includes:

[0029] The pull rod is threadedly connected to the base, and the end portion is rotatably connected to the piston.

[0030] By adopting the above technical solution, the pull rod is rotated, and the pull rod pulls the piston to move. The piston moves slowly, so that the cell culture fluid and seed cells can be slowly drawn into the bone matrix gelatin scaffold, avoiding the cell culture fluid and seed cells from entering under the bone matrix gelatin scaffold.

[0031] More preferably, it also includes:

[0032] The receiving plate is fixedly connected to the upper supporting ring, arranged inside the upper supporting ring, and is used for containing the cell culture fluid dripping from the bone grafting matrix gelatin.

[0033] By adopting the above technical solution, the cell culture fluid dripping from the bone matrix gelatin can be received to prevent the cell culture fluid from contaminating the piston and the inside of the piston hole.

[0034] More preferably, it also includes:

[0035] The through hole penetrates the receiving plate and is arranged on the side wall of the receiving plate, and is used to connect the openings at both ends of the upper support ring.

[0036] By adopting the above technical solution, the through hole allows gas to pass through when the piston moves. The through hole is set on the side wall of the receiving plate to prevent cell fluid from passing through the through hole, further avoiding cell culture fluid from contaminating the piston and the inside of the piston hole.

[0037] More preferably, it also includes:

[0038] The inner hole wall of the convex ring contacts and cooperates with the outer side wall of the elastic ring.

[0039] By adopting the above technical solution, the convex ring limits the outer wall of the elastic ring, reduces the deformation of the elastic ring toward the outside of the side wall, and increases the deformation of the inner hole of the elastic ring.

[0040] More preferably, the pressing tube is made of a transparent material.

[0041] By adopting the above technical solution, the transparent compression tube is convenient for observation during cell culture.

[0042] In summary, compared with the prior art, the utility model has the following beneficial effects: when performing cell culture, the bone matrix gelatin is trimmed into a cylindrical shape, the diameter of the trimmed cylindrical bone matrix gelatin is approximately equal to the diameter of the inner hole of the elastic ring, the trimmed bone matrix gelatin is inserted into the elastic ring, the outer wall of the bone matrix gelatin contacts the inner hole wall of the elastic ring, the elastic ring is then placed on the base, the lower end face of the elastic ring contacts the base, the pressure tube is placed on the upper end of the elastic ring, the base and the pressure tube are brought close to each other through the pressure assembly, so that the pressure tube and the base squeeze the elastic ring, the elastic ring deforms and the inner hole diameter is reduced, the inner hole wall of the elastic ring and the side wall of the bone matrix gelatin squeeze each other, the gap between the inner hole wall of the elastic ring and the side wall of the bone matrix gelatin is sealed, and finally the cell culture fluid and seed cells are injected into the pressure tube to perform cell culture. The pressure assembly can use a counterweight block, the counterweight block is fixed to the pressure tube, and pressure is applied to the elastic ring by gravity, so that the elastic ring is deformed. The pressure assembly may also need an elastic member, which is connected to the pressure tube and the base respectively, and the elastic force of the elastic member applies pressure to the elastic ring. Only the elastic member with built-in bone matrix gelatin needs to be placed on the base, and the sealing of the edge gap of the bone matrix gelatin can be achieved by the compression of the pressure tube and the base, thereby improving the efficiency of cell culture. The sealing of the edge gap of the bone matrix gelatin is achieved by the deformation of the elastic ring, and the inner wall of the elastic ring can be integrally fitted with the outer wall of the bone matrix gelatin, thereby achieving the overall sealing of the height direction of the bone matrix gelatin, and the sealing property will not weaken with the change of the cell culture duration, so the cell growth process is difficult to climb out of the culture hole, and will not grow below the bone matrix gelatin material, thereby improving the success rate of culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 It is a structural schematic diagram of this embodiment;

[0044] Figure 2 Schematic diagram of the internal structure of this embodiment;

[0045] Figure 3 Schematic diagram of the internal planar structure of this embodiment;

[0046] Figure numerals: 1-elastic ring; 2-base; 21-upper support ring; 22-lower support seat; 3-pressure tube; 4-piston hole; 5-piston; 6-first thread; 7-second thread; 8-sealing ring; 9-pull rod; 10-receiving plate; 11-through hole; 12-convex ring; 13-bone matrix gelatin. DETAILED DESCRIPTION

[0047] The following is combined with Figure 1 —3 The utility model is further introduced in detail.

[0048] A petri dish is a laboratory vessel used for microbial or cell culture, usually made of glass or plastic. There are basically two types of petri dish materials, mainly plastic and glass. Glass can be used for plant materials, microbial culture and animal cell adherent culture. Plastic may be made of polyethylene, which can be disposable or reusable. It is suitable for laboratory inoculation, streaking, and bacterial isolation operations, and can be used for the culture of plant materials.

[0049] Bone matrix gelatin is a scaffold material obtained by removing minerals and cells from bones using chemical and physical methods. BMG is a porous biomaterial similar to a sponge. Cells can be planted, migrate, proliferate and differentiate on the surface and inside of BMG. At the same time, the nutrients required for cell culture and the macromolecules secreted by the cells themselves can also freely pass through the inside of BMG. More importantly, BMG has a wide range of sources, has no or very low antigenicity, and can be naturally absorbed in the body without surgical removal. Articular cartilage has been successfully constructed in vitro using BMG as a biological scaffold, and tissue-engineered cartilage constructed in vitro has been transplanted into the body to repair articular cartilage defects. In addition, a large amount of clinical experimental evidence suggests that BMG can be effectively used in the treatment of bone defects.

[0050] Chinese patent publication number: CN 956456102 A discloses a method for constructing epiphyseal cartilage in vitro, including: 1) culturing human chondrocyte cell line or human bone marrow stem cell to form a cell monolayer, digesting with trypsin, washing, resuspending to obtain a cell suspension for counting and standby; 2) cutting the pre-treated bone matrix gelatin material into a cylinder smaller than the diameter of the culture hole, and drilling a hole through the two bottom surfaces of the cylinder on the cylinder, sterilizing; suspending and fixing the cut bone matrix gelatin material in the culture hole; 3) implanting the cell suspension obtained in step 1) through the hole below the bone matrix gelatin material, and making the liquid surface infiltrate the bone matrix gelatin material, and culturing to obtain epiphyseal cartilage. This method establishes a culture system of C221 / I2 cells and a method for constructing epiphyseal cartilage in vitro with the cell suspension, solving the problem of difficulty in the source of seed cells; the cell properties are stable and the culture cost is low. Preferably, the method for constructing epiphyseal cartilage in vitro as described above, in step 3), before implanting the cell suspension, further comprises: sealing the gap between the bone matrix gelatin material and the edge of the culture well with sterile agarose gel. Sealing the gap between the bone matrix gelatin material and the edge of the culture well can prevent the cells from crawling out of the culture well during growth, and only grow under the bone matrix gelatin material.

[0051] Regarding the above-mentioned related technologies, when using the culture method, the gap between the bone matrix gelatin and the culture well needs to be sealed. However, when the gap between the bone matrix gelatin material and the edge of the culture well is filled by the sterile agarose gel sealing method, since the gap has a certain depth, it takes a lot of time to fill the gap, which reduces the efficiency of cell culture. At the same time, during the cell culture process, the gel is immersed in the cell culture solution for a long time and is easily separated from the gap. During the cell growth process, the cells crawl out of the culture well and grow under the bone matrix gelatin material, which reduces the success rate of cell culture.

[0052] Based on the above technical problems, the applicant has conceived the following technical solutions:

[0053] The elastic squeezing effect of the elastic member is used to seal the edge of the bone matrix gelatin.

[0054] Based on the above concept, the applicant proposed the technical solution of this application, which is as follows:

[0055] A cell culture dish, such as Figure 1 , Figure 2 as well as Figure 3As shown, it includes: an elastic ring 1, whose inner hole wall contacts the side wall of the bone matrix gelatin 13, and is used to coat the bone matrix gelatin 13. A base 2 contacts the lower end surface of the elastic ring 1, and is used to support the elastic ring 1. A pressure tube 3, whose end contacts the upper end surface of the elastic ring 1, and the interior is used to accommodate cell culture fluid and seed cells. A pressure component is connected to the base 2 and the pressure tube 3, and is used to make the base 2 and the pressure tube 3 approach each other, and the pressure tube 3 and the base 2 squeeze the elastic ring 1, and the elastic ring 1 is deformed and the inner hole diameter is reduced, so that the inner hole wall of the elastic ring 1 and the side wall of the bone matrix gelatin 13 are squeezed against each other, and the gap between the inner hole wall of the elastic ring 1 and the side wall of the bone matrix gelatin 13 is sealed.

[0056] When cell culture is performed, the bone matrix gelatin 13 is trimmed into a cylindrical shape, the diameter of the trimmed cylindrical bone matrix gelatin 13 is approximately equal to the diameter of the inner hole of the elastic ring 1, the trimmed bone matrix gelatin 13 is inserted into the elastic ring 1, the outer wall of the bone matrix gelatin 13 is in contact with the inner hole wall of the elastic ring 1, the elastic ring 1 is then placed on the base 2, the lower end face of the elastic ring 1 is in contact with the base 2, the pressing tube 3 is placed on the upper end of the elastic ring 1, the base 2 and the pressing tube 3 are brought close to each other by the pressure assembly, so that the pressing tube 3 and the base 2 squeeze the elastic ring 1, the elastic ring 1 is deformed and the inner hole diameter is reduced, the inner hole wall of the elastic ring 1 and the side wall of the bone matrix gelatin 13 are squeezed each other, the gap between the inner hole wall of the elastic ring 1 and the side wall of the bone matrix gelatin 13 is sealed, and finally the cell culture fluid and seed cells are injected into the pressing tube 3 to perform cell culture. The pressure assembly can use a counterweight block, the counterweight block is fixed to the pressing tube 3, and pressure is applied to the elastic ring 1 by gravity, so that the elastic ring 1 is deformed. The pressure assembly may also need to use an elastic member, which is respectively connected to the pressure tube 3 and the base 2, and applies pressure to the elastic ring 1 through the elastic force of the elastic member.

[0057] During cell culture, only the elastic member with built-in bone matrix gelatin 13 needs to be placed on the base 2, and the sealing of the edge gap of the bone matrix gelatin 13 can be achieved by compression of the pressure tube 3 and the base 2, thereby improving the efficiency of cell culture. The sealing of the edge gap of the bone matrix gelatin 13 is achieved by deformation of the elastic ring 1, and the inner wall of the elastic ring 1 can be integrally fitted with the outer wall of the bone matrix gelatin 13, thereby achieving the overall sealing of the height direction of the bone matrix gelatin 13, and the sealing property will not weaken with the change of the cell culture duration, so it is difficult for the cells to climb out of the culture hole during the growth process, and they will not grow under the bone matrix gelatin 13 material, thereby improving the success rate of the culture.

[0058] Specifically, the method further includes: a piston hole 4, which is arranged inside the base 2 and communicated with the pressure tube 3. A piston 5 is slidably sleeved with the piston hole 4, and is used to form a negative pressure between the piston 5 and the bone matrix gelatin 13 inside the piston hole 4. After adding cell culture fluid and seed cells inside the pressure tube 3, the piston 5 is pushed away from the bone matrix gelatin 13, and a negative pressure is formed between the piston 5 and the bone matrix gelatin 13 inside the piston hole 4, and the cell culture fluid and seed cells are sucked into the pores of the bone matrix gelatin 13, so that the seed cells quickly adhere to the bone matrix gelatin 13 scaffold and grow, further improving the efficiency of cell culture.

[0059] Specifically, the pressure assembly includes: a first thread 6, which is arranged on the base 2. A second thread 7, which is arranged on the pressure tube 3, cooperates with the first thread 6. The base 2 and the pressure tube 3 rotate relative to each other. Under the action of the first thread 6 and the second thread 7, the base 2 and the pressure tube 3 approach each other to compress the elastic ring 1. The thread engagement can slowly increase the pressure on the elastic member, so that the elastic ring 1 slowly deforms, avoiding excessive pressure of the elastic ring 1 on the bone matrix gelatin 13 bracket, and preventing the bone matrix gelatin 13 from rupturing.

[0060] Specifically, the base 2 is divided into: an upper support ring 21, which contacts the lower end surface of the elastic ring 1 and is used to support the elastic ring 1. A lower support seat 22, which contacts the end of the upper support ring 21 away from the elastic ring 1, a first thread 6 is provided on the lower support seat 22, and a piston hole 4 is provided in the lower support seat 22. The lower support seat 22 is rotated, the lower support seat 22 and the upper support ring 21 rotate relative to each other, the lower support seat 22 and the pressure tube 3 rotate relative to each other, the lower support seat 22 pushes the upper support ring 21 and the pressure tube 3 to approach each other, thereby compressing the elastic ring 1, and the upper support ring 21 and the pressure tube 3 do not rotate relative to each other, thereby avoiding wear on the elastic ring 1 and improving the service life of the elastic ring 1.

[0061] Specifically, it also includes: a sealing ring 8, which is arranged between the upper support ring 21 and the lower support seat 22, and is used to seal the contact position between the upper support ring 21 and the lower support seat 22. The sealing ring 8 is used to seal the contact position between the upper support ring 21 and the lower support seat 22 to prevent air leakage when the piston 5 moves.

[0062] Specifically, it also includes: a pull rod 9, which is threadedly connected to the base 2, and the end of which is rotatably connected to the piston 5. By rotating the pull rod 9, the pull rod 9 pulls the piston 5 to move, and the piston 5 moves slowly, so that the cell culture fluid and seed cells can be slowly drawn into the bone matrix gelatin 13 scaffold, and the cell culture fluid and seed cells are prevented from entering the bottom of the bone matrix gelatin 13 scaffold.

[0063] Specifically, it also includes: a receiving plate 10, which is fixedly connected to the upper support ring 21 and arranged inside the upper support ring 21, and is used to hold the cell culture fluid dripping from the bone matrix gelatin 13. The receiving plate 10 receives the cell culture fluid dripping from the bone matrix gelatin 13 to prevent the cell culture fluid from contaminating the piston 5 and the inside of the piston hole 4.

[0064] Specifically, it also includes: a through hole 11 that passes through the receiving plate 10 and is arranged on the side wall of the receiving plate 10, and is used to connect the openings at both ends of the upper support ring 21. The through hole 11 allows gas to pass through when the piston 5 moves. The through hole 11 is arranged on the side wall of the receiving plate 10 to prevent cell fluid from passing through the through hole 11, and further prevent the cell culture fluid from contaminating the piston 5 and the inside of the piston hole 4.

[0065] Specifically, it also includes: a convex ring 12, whose inner hole wall contacts and cooperates with the outer wall of the elastic ring 1. The convex ring 12 limits the outer wall of the elastic ring 1, reduces the deformation of the elastic ring 1 toward the outer side of the side wall, and increases the deformation of the inner hole of the elastic ring 1.

[0066] Specifically, the pressing tube 3 is made of a transparent material, which is convenient for observation during cell culture.

[0067] Working principle and process

[0068] Please combine Figure 1 - Figure 3 , the principle process of the present invention is described in detail as follows:

[0069] When cell culture is performed, the bone matrix gelatin 13 is trimmed into a cylindrical shape, the diameter of the trimmed cylindrical bone matrix gelatin 13 is approximately equal to the diameter of the inner hole of the elastic ring 1, the trimmed bone matrix gelatin 13 is inserted into the elastic ring 1, the outer wall of the bone matrix gelatin 13 is in contact with the inner hole wall of the elastic ring 1, and then the elastic ring 1 is placed on the base 2, the lower end face of the elastic ring 1 is in contact with the base 2, the pressing tube 3 is placed on the upper end of the elastic ring 1, the lower support seat 22 is rotated, the lower support seat 22 and the upper support ring 21 are relatively rotated, the lower support seat 22 and the pressing tube 3 are relatively rotated, and the lower support seat 22 pushes the upper support ring 21 and the pressing tube 3 to approach each other Thereby, the elastic ring 1 is compressed, and the elastic ring 1 is deformed to reduce the diameter of the inner hole, so that the inner hole wall of the elastic ring 1 and the side wall of the bone matrix gelatin 13 are squeezed against each other, so that the gap between the inner hole wall of the elastic ring 1 and the side wall of the bone matrix gelatin 13 is sealed, and finally, the cell culture fluid and seed cells are injected into the pressing tube 3. After the cell culture fluid and seed cells are added into the pressing tube 3, the pull rod 9 is rotated, and the pull rod 9 pulls the piston 5 to move, and the piston 5 moves away from the bone matrix gelatin 13, and a negative pressure is formed between the piston 5 and the bone matrix gelatin 13 inside the piston hole 4, and the cell culture fluid and seed cells are sucked into the pores of the bone matrix gelatin 13 for cell culture.

[0070] This specific embodiment is merely an explanation of the utility model, and it is not a limitation of the utility model. After reading this specification, those skilled in the art can make non-creative modifications to the embodiment as needed, but as long as it is within the protection scope of the utility model, it is protected by the patent law.

Claims

1. A cell culture dish, characterized in that: include: An elastic ring (1), whose inner hole wall contacts the side wall of the bone matrix gelatin (13) and is used to coat the bone matrix gelatin (13); A base (2) in contact with the lower end surface of the elastic ring (1) and used to support the elastic ring (1); A pressing tube (3), the end of which is in contact with the upper end surface of the elastic ring (1), and the interior of which is used to contain cell culture fluid and seed cells; A pressure assembly is connected to the base (2) and the pressure tube (3) and is used to bring the base (2) and the pressure tube (3) closer to each other, so that the pressure tube (3) and the base (2) squeeze the elastic ring (1), and the elastic ring (1) deforms to reduce the diameter of the inner hole, thereby squeezing the inner hole wall of the elastic ring (1) and the side wall of the bone matrix gelatin (13) against each other, and sealing the gap between the inner hole wall of the elastic ring (1) and the side wall of the bone matrix gelatin (13).

2. The cell culture dish according to claim 1, characterized in that Also includes: A piston hole (4) is provided inside the base (2) and is connected to the pressure tube (3); The piston (5) is slidably sleeved with the piston hole (4) to form negative pressure between the piston (5) inside the piston hole (4) and the bone matrix gelatin (13).

3. The cell culture dish according to claim 2, characterized in that The pressure assembly comprises: A first thread (6) is arranged on the base (2); A second thread (7) is provided on the pressure tube (3) and is matched with the first thread (6).

4. The cell culture dish according to claim 3, characterized in that The base (2) is divided into: An upper support ring (21), in contact with the lower end surface of the elastic ring (1) and used for supporting the elastic ring (1); The lower support seat (22) contacts the end of the upper support ring (21) away from the elastic ring (1), the first thread (6) is arranged on the lower support seat (22), and the piston hole (4) is arranged in the lower support seat (22).

5. The cell culture dish according to claim 4, characterized in that: Also includes: A sealing ring (8) is provided between the upper support ring (21) and the lower support seat (22) and is used for sealing the contact position between the upper support ring (21) and the lower support seat (22).

6. The cell culture dish according to claim 2, characterized in that: Also includes: The pull rod (9) is threadedly connected to the base (2), and the end portion is rotatably connected to the piston (5).

7. The cell culture dish according to claim 4, characterized in that: Also includes: The receiving plate (10) is fixedly connected to the upper supporting ring (21), is arranged inside the upper supporting ring (21), and is used to receive the cell culture fluid dripping from the bone matrix gelatin (13).

8. The cell culture dish according to claim 7, characterized in that: Also includes: A through hole (11) passes through the receiving plate (10), is provided on a side wall of the receiving plate (10), and is used to connect openings at both ends of the upper support ring (21).

9. The cell culture dish according to claim 1, characterized in that: Also includes: The inner hole wall of the convex ring (12) contacts and cooperates with the outer side wall of the elastic ring (1).

10. The cell culture dish according to claim 1, characterized in that: The pressing tube (3) is made of a transparent material.

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