Monitoring device for cell culture

Abstract

The utility model discloses a monitoring device for cell culture, which is characterized in that culture dishes are placed one by one through windows, stable placement of the culture dishes is guaranteed through in-groove clamping structures in placement grooves, and the culture dishes in each placement groove can be rotated to a position below a camera shooting end of a cell monitor through arrangement of a rotation driver; the cell culture information of the culture dish is collected through the cell monitor and displayed on the display, meanwhile, photographing and recording are carried out, the cell culture state is monitored under the condition that the culture dish is not taken out, the temperature in the placing barrel can be adjusted through the arrangement of the refrigerator and the heating part, and the good culture temperature is maintained.

Description

Technical Field

[0001] The utility model relates to the field of biotechnology, and particularly relates to a monitoring device for cell culture. Background Art

[0002] Cell culture is widely used in the field of biotechnology. It is a method that usually simulates the in-vivo environment in vitro, including aseptic conditions, appropriate temperature, pH value, and certain nutrient conditions, to enable cells to survive, grow, reproduce, and maintain their main structures and functions.

[0003] After using a culture dish to hold cells, they will be uniformly placed in a placement device. Since it is necessary to monitor the cell culture situation in real time, the culture dish needs to be taken out and observed regularly. During the process of taking and placing, the cells inside the culture dish are likely to shake, reducing the growth effect. At the same time, the simple placement device cannot adjust the internal temperature. Content of the Utility Model

[0004] In view of the problems existing in the prior art, the utility model discloses a monitoring device for cell culture. The technical solution adopted is as follows: it includes a cell monitor, a cavity plate, a refrigerator, and a refrigerator air outlet. A cell monitor is arranged at the rear side of the top surface of the placement cylinder, and its monitoring and imaging end extends into the placement cylinder and faces directly downward. The cell monitor mainly used is to transmit the cell state to a display for display and take pictures for recording. The refrigerator is a prior art, and it outputs cold air to achieve the purpose of reducing temperature. The remaining parts also include a placement cylinder, a base, a rotation drive, a window, a window sealing plate, an upper opening, a display, a heating part, a rotating placement plate, placement grooves, a bottom partition plate, and a clamping structure inside the grooves, which are used to clamp the culture dish placed inside. The base is distributed at the bottom of the placement cylinder, and the refrigerator is installed on the bottom surface. A cavity plate is fixed at the lower end inside. The refrigerator air outlets are evenly distributed on the cavity plate and are connected to the output end of the refrigerator at the lower part. The cold air output by the refrigerator passes through the cavity plate and the refrigerator air outlets in sequence and enters the placement cylinder to realize temperature reduction for energy supply. In order to control the temperature inside the placement cylinder in real time, a temperature sensor is arranged inside the placement cylinder and the temperature state is displayed on the display screen. A rotation drive is arranged on the side of the placement cylinder, and the rotating placement plate inside the placement cylinder is rotationally installed through it. By starting the rotation drive, the rotating placement plate can be rotated. A window is opened on the front side of the placement cylinder, and a through upper opening is opened in the upper part of the window. The window sealing plate is inserted through the upper opening to close the through hole. A buckling groove is opened at the bottom surface in the window. After the window sealing plate is inserted, its lower end can be inserted into the buckling groove. A display and a heating part are installed on the placement cylinder. The placement grooves are evenly distributed on the rotating placement plate. The placement grooves are through-shaped, and an annular bottom partition plate is integrally formed at the bottom opening. A clamping structure inside the groove is arranged on the inner wall of the placement groove to clamp the culture dish placed inside.

[0005] As a preferred technical solution of the present utility model, the rotation drive includes an installation box, a stepper motor, a sidewall annular groove, and a gear. A sidewall annular groove is formed on the inner sidewall of the placement cylinder. An installation box is fixedly arranged on the outer side of the right side of the placement cylinder, and the installation box communicates with the sidewall annular groove. A gear is rotatably installed in the installation box and is driven by the stepper motor installed on the installation box. The rotation placement plate is movably placed through the cooperation of the sidewall annular groove. Tooth grooves are distributed on its arc-shaped outer sidewall, and the tooth grooves are meshed with the gear. After the stepper motor is started, it drives the gear to rotate. Under the meshing with the tooth grooves, it can drive the rotation placement plate to rotate, and different placement grooves can be rotated to the lower part of the monitoring end of the cell monitor.

[0006] As a preferred technical solution of the present utility model, the heating part includes a left heater, a right heater, and a heating pipe. Heating pipes are distributed on the inner wall of the placement cylinder, and the left and right heating pipes are respectively powered by the left heater and the right heater installed on the top of the placement cylinder.

[0007] As a preferred technical solution of the present utility model, the clamping structure in the groove includes an inner groove opening, a spring, and a clamping block. An inner groove opening is formed on the inner wall of the placement groove. A spring is fixed in the inner groove opening. The clamping block is placed in the inner groove opening in a matching manner and is fixedly connected with the spring. The upper part of the part of the clamping block extending out of the inner groove opening is inclined. When the culture dish is inserted from the window and placed downward, it can squeeze the clamping block under the action of the inclination. After the culture dish is completely placed and its bottom contacts the annular bottom partition board, the inner end of the clamping block clamps the sidewall of the culture dish under the action of the spring.

[0008] As a preferred technical solution of the present utility model, the stepper motor, the cell monitor, the left heater, the right heater, the cavity plate, and the refrigerator are all externally connected to a power supply for use.

[0009] The beneficial effects of the present utility model are as follows: The culture dishes are placed one by one through the window, and the stable placement of the culture dishes is ensured by the clamping structure in the groove of the placement groove. Through the setting of the rotation drive, the culture dishes in each placement groove can be rotated to the lower part of the camera end of the cell monitor. The cell culture information of the culture dishes is collected by the cell monitor and displayed on the display and photographed and recorded at the same time. The cell culture state is monitored without taking out the culture dishes. Through the setting of the refrigerator and the heating part, the temperature in the placement cylinder can be adjusted to maintain a good culture temperature. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 is a schematic structural diagram of the present utility model;

[0011] Figure 2 is a schematic cross-sectional structural diagram of the present utility model;

[0012] Figure 3 is a schematic cross-sectional structural diagram of some components of the present utility model.

[0013] In the figure: placing cylinder 1, base 2, mounting box 3, stepping motor 4, window 5, window sealing plate 6, upper opening 8, cell monitor 9, display 10, left heater 11, right heater 12, side wall annular groove 13, rotating placing plate 14, gear 15, placing groove 16, bottom partition 17, inner notch 18, spring 181, clamping block 19, heating pipe 20, cavity plate 21, cooler 22, cooler air outlet 23. Specific implementation mode

[0014] Embodiment 1

[0015] As Figures 1 to 3 shown, the utility model discloses a monitoring device for cell culture. The adopted technical solution is that it includes a cell monitor 9, a cavity plate 21, a cooler 22 and a cooler air outlet 23. The remaining parts also include a placing cylinder 1, a base 2, a rotation drive, a window 5, a window sealing plate 6, an upper opening 8, a display 10, a heating part, a rotating placing plate 14, a placing groove 16, a bottom partition 17 and a clamping structure in the groove. The bottom of the placing cylinder 1 is distributed with a base 2, the cooler 22 is installed on the bottom surface, the cavity plate 21 is fixed at the lower end inside, the cooler air outlets 23 are evenly distributed on the cavity plate 21, and the lower part is communicated with the output end of the cooler 22. The cell monitor 9 is arranged at the rear side of the top surface of the placing cylinder 1. A rotation drive is arranged on the side surface of the placing cylinder 1 to rotatably install the rotating placing plate 14 in the placing cylinder 1. A window 5 is opened on the front side of the placing cylinder 1, and a through upper opening 8 is opened in the upper part of the window 5. The window sealing plate 6 is inserted through the upper opening 8 to close the through hole 5. A buckling groove 7 is opened at the bottom surface in the window 5. The display 10 and the heating part are installed on the placing cylinder 1. The placing grooves 16 are evenly distributed on the rotating placing plate 14. The placing grooves 16 are through-shaped, and an annular bottom partition 17 is integrally formed at the bottom opening thereof. A clamping structure in the groove is arranged on the inner wall of the placing groove 16.

[0016] As a preferred technical solution of the utility model, setting the rotation drive can make the rotating placing plate 14 rotate so that different placing grooves can all rotate to the directly below the imaging end of the cell monitor 9 for monitoring the culture state. Its specific structure includes a mounting box 3, a stepping motor 4, a side wall annular groove 13 and a gear 15. The side wall annular groove 13 is opened on the inner side wall of the placing cylinder 1. The mounting box 3 is fixed outside the right side of the placing cylinder 1. The mounting box 3 is communicated with the side wall annular groove 13. The gear 15 is rotatably installed in the mounting box 3 and is driven by the stepping motor 4 installed on the mounting box 3. The rotating placing plate 14 is movably placed through the cooperation of the side wall annular groove 13. Tooth grooves are distributed on its arc-shaped outer side wall, and the tooth grooves are meshed with the gear 15.

[0017] As a preferred technical solution of the present utility model, the heating part includes a left heater 11, a right heater 12 and a heating pipe 20. The heating pipe 20 is distributed on the inner wall of the placing cylinder 1, and the left and right heating pipes 20 are respectively powered by the left heater 11 and the right heater 12 installed on the top of the placing cylinder 1.

[0018] As a preferred technical solution of the present utility model, in order to ensure that the culture dish is always stable when placed in the placing groove, a clamping structure in the groove is provided. Its specific structure includes an inner groove opening 18, a spring 181 and a clamping block 19. The inner wall of the placing groove 16 is provided with the inner groove opening 18, the spring 181 is fixed in the inner groove opening 18, the clamping block 19 is fitted in the inner groove opening 18 and fixedly connected with the spring 181, and the upper part of the part of the clamping block 19 extending out of the inner groove opening 18 is inclined.

[0019] As a preferred technical solution of the present utility model, the stepper motor 4, the cell monitor 9, the left heater 11, the right heater 12, the cavity plate 21 and the cooler 22 are all externally connected to a power supply for use, or the battery power supply method can also be adopted.

[0020] The working principle of the present utility model: When in use, the culture dishes are successively placed into the placing cylinder 1 through the window, and under the start of the stepper motor 4, each culture dish is placed in different placing grooves 16. After the culture dish is placed in the placing groove 16, manually press it down. The clamping block 19 is squeezed and retracted under the action of the inclined part to squeeze the spring 181. After the culture dish is completely placed, the clamping block 19 presses against the culture dish under the action of the spring 181 to maintain its stable placement. After the placement is completed, the window sealing plate 6 is inserted downward through the upper opening 8 to close the window 5. The temperature in the placing cylinder is adjusted by the cooler 22, the left heater 11 and the right heater 12. Through the rotation drive setting, the culture dish in each placing groove can be rotated to the lower part of the camera end of the cell monitor. The cell culture information of the culture dish is collected by the cell monitor and displayed on the display and photographed and recorded at the same time, and the cell culture state is monitored without taking out the culture dish.

[0021] The components not described in detail in this article are prior art.

[0022] Although the specific embodiments of the present utility model are described in detail above, the present utility model is not limited to the above embodiments. Within the scope of knowledge possessed by those of ordinary skill in the art, various changes can be made without departing from the purpose of the present utility model, and the modifications or deformations without creative labor are still within the protection scope of the present utility model.

Claims

1. A monitoring device for cell culture, comprising a cell monitor (9), a cavity plate (21), a refrigerator (22) and a refrigerator air outlet (23), characterized in that: The invention comprises a placement tube (1), a base (2), a rotation drive, a window (5), a window sealing plate (6), an upper opening (8), a display (10), a heating part, a rotation placement plate (14), a placement groove (16), a bottom blocking plate (17) and a clamping structure in the groove; the placement tube (1) has a base (2) at the bottom, a refrigerator (22) is installed on the bottom surface, and a cavity plate (21) is fixed at the lower end inside; refrigerator air outlets (23) are evenly distributed on the cavity plate (21), and the lower part is connected to the output end of the refrigerator (22); a cell monitor (9) is arranged at the rear side of the top surface of the placement tube (1); the placement tube (1 ) is provided with a rotation drive on the side, through which a rotating placement plate (14) in the placement tube (1) is rotatably installed; a window (5) is opened on the front side of the placement tube (1), and a through upper opening (8) is opened in the upper part of the window (5), and a window sealing plate (6) is inserted through the upper opening (8) to seal the window (5); a display (10) and a heating part are installed on the placement tube (1); placement grooves (16) are evenly distributed on the rotating placement plate (14), and the placement grooves (16) are through-shaped, and an annular bottom blocking plate (17) is integrally formed at the bottom opening; the inner wall of the placement groove (16) is provided with an in-groove clamping structure.

2. A monitoring device for cell culture according to claim 1, characterized in that: The rotation drive comprises a mounting box (3), a stepping motor (4), a side wall annular groove (13) and a gear (15); the inner side wall of the placement tube (1) is provided with a side wall annular groove (13); a mounting box (3) is fixed to the right side of the placement tube (1), and the mounting box (3) is connected to the side wall annular groove (13); a rotationally mounted gear (15) is installed in the mounting box (3), and is driven by a stepping motor (4) installed on the mounting box (3); the rotation placement plate (14) is movably placed in cooperation with the side wall annular groove (13), and tooth grooves are distributed on its arc-shaped outer side wall, and the tooth grooves and the gear (15) are meshed with each other.

3. A monitoring device for cell culture according to claim 1, characterized in that: A buckling groove (7) is provided on the bottom surface of the window (5).

4. A monitoring device for cell culture according to claim 2, characterized in that: The heating portion comprises a left heater (11), a right heater (12) and a heating tube (20); the heating tube (20) is distributed on the inner wall of the placement tube (1), and the left and right heating tubes (20) are respectively powered by the left heater (11) and the right heater (12) installed on the top of the placement tube (1).

5. A monitoring device for cell culture according to claim 1, characterized in that: The in-slot clamping structure comprises an inner slot (18), a spring (181) and a clamping block (19); the inner wall of the placement slot (16) is provided with an inner slot (18); a spring (181) is fixed in the inner slot (18); the clamping block (19) is placed in the inner slot (18) and is fixedly connected to the spring (181); the upper part of the clamping block (19) extending from the inner slot (18) is inclined.

6. A monitoring device for cell culture according to claim 4, characterized in that: The stepper motor (4), the cell monitor (9), the left heater (11), the right heater (12), the cavity plate (21) and the refrigerator (22) are all connected to an external power source for use.

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