Anti-hemolysis interference detection card

Abstract

The utility model relates to an anti-hemolysis interference detection card and belongs to the technical field of medical detection. According to the utility model, the interception part is arranged between the filtration chromatography structure and the water absorption pad, after blood cells leak, hemoglobin is intercepted through the interception part, and the hemoglobin is removed by combining a hemoglobin antibody with the hemoglobin, so that the interference of hemolysis on a test result is avoided; meanwhile, a black shading plate is further arranged above the water absorption pad, so that the background color of the reaction layer can be ensured to be constant and consistent, and the accuracy of light source detection is improved.

Description

Technical Field

[0001] The utility model relates to a detection card for anti-hemolysis interference, belonging to the technical field of medical detection. Background Art

[0002] At present, the test cards for detecting anti-hemolysis interference in the prior art are mainly divided into two types. One is a vertical test card (dry chemical method), and its structure from top to bottom is successively composed of a diffusion layer, a blood filtration layer, a filtering layer and a reaction layer; it can enable whole blood samples to sink layer by layer under the action of gravity, and finally a Trinder reaction occurs and colors in the reaction layer. The other is a vertical + chromatographic high-density lipoprotein test card (dry chemical method), and its structure from top to bottom is successively composed of a filtering layer, a separation layer, a reaction layer and a detection layer. The quantum dot line of the detection layer is located outside the covering area. Through chromatographic action, the reaction substrate is aggregated to the position of the quantum dot line and reacts, and the fluorescence signal is detected to quantify.

[0003] However, blood cells in the samples of these test cards are prone to leakage or rupture between membranes under the pressure of the membrane material during the sample addition process, and these test cards detect the strength of the color at the reaction end point. The color of the leaked blood cells or the hemoglobin released by rupture itself will interfere with the detection result; at the same time, the background color of the reaction layer changes after blood leakage, which will cause interference during the end point color detection, resulting in inaccurate results. Therefore, there are many drawbacks.

[0004] Another example is the dry chemical test card structure shown in CN116338211A. Although this test card can detect hemoglobin and alanine aminotransferase in the sample, there are detection defects. In the alanine aminotransferase detection area, it is impossible to detect samples that have undergone hemolysis, which greatly limits its application. Summary of the Utility Model

[0005] To solve the above problems, the utility model provides a detection card for anti-hemolysis interference, including:

[0006] A bottom plate, at least a part of the upper surface of the bottom plate is provided with a filtration and chromatography structure, and the filtration and chromatography structure includes a diffusion layer, a blood filtration layer, a chromatography pad and a support layer that are successively overlapped and arranged on the upper surface of the bottom plate from top to bottom; the support layer has a notch at the position corresponding to the reaction hole of the bottom plate, the notch is located below the chromatography pad and is completely covered by the chromatography pad, and a reaction layer and a treatment layer are successively adhered to the notch from top to bottom.

[0007] A water absorption pad, at least a part of the water absorption pad is adhered above the support layer and at least a part of it is adhered above the chromatography pad.

[0008] Further, the diffusion layer is a wire mesh with a mesh density of 50 - 1000 mesh, and the material of the diffusion layer is one of nylon film, polyester film, polyethylene film, and paper material.

[0009] Further, the material of the blood filtration layer is one of polyester film, polyethersulfone membrane, and cellulose acetate filter membrane.

[0010] Further, the material of the chromatography pad is nitrocellulose membrane. The chromatography pad is provided with a retention part, which divides the chromatography pad into a first chromatography part and a second chromatography part. The diffusion layer and the blood filtration layer only cover the upper part of the first chromatography part, and the water absorption pad only covers a partial area above the second chromatography part; the surface of the retention part is coated with a coating solution containing hemoglobin antibody, and the material of the retention part is nitrocellulose membrane.

[0011] Further, the material of the support layer is one of silica gel and polytetrafluoroethylene.

[0012] Further, the material of the treatment layer is one of polyester film, polyethersulfone membrane, and glass fiber membrane.

[0013] Further, the material of the reaction layer is one of polyester film, polyethersulfone membrane, and glass fiber membrane.

[0014] Further, a single - color light - shielding plate is arranged above the water absorption pad. One part of the light - shielding plate is adhered above the water absorption pad, and the other part is adhered above the chromatography pad.

[0015] Further, the color of the light - shielding plate is black, and the material of the light - shielding plate is one of polyvinyl chloride film, polyethylene film, and polyester film.

[0016] Further, the transmittance of the light - shielding plate is between 0 and 55%.

[0017] Advantages of the present utility model:

[0018] By arranging a retention part between the filtration chromatography structure and the water absorption pad, after blood cells leak, the hemoglobin is intercepted by the retention part, and the hemoglobin is removed by combining hemoglobin with hemoglobin antibody, thus avoiding the interference of hemolysis and reagent leakage between the upper and lower layers on the test results; meanwhile, the present utility model also has a black light - shielding plate arranged above the water absorption pad, which can ensure that the background color of the reaction layer is constant and consistent, and improve the accuracy of light source detection. Description of the drawings

[0019] Figure 1 It is a schematic diagram of the overall structure in an embodiment of the present utility model;

[0020] Figure 2An exploded view of the overall structure in an embodiment of the present utility model;

[0021] In the figure: 1. Diffusion layer; 2. Blood filtration layer; 3. Chromatographic pad; 4. Support layer; 5. Interception part; 6. Light-shielding plate; 7. Absorbent pad; 8. Bottom plate; 9. Reaction hole; 10. Reaction layer; 11. Treatment layer. Specific embodiments

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are some but not all of the embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the protection scope of the present utility model.

[0023] In the description of the present utility model, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present utility model. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance. Among them, the terms "first position" and "second position" are two different positions.

[0024] In the description of the present utility model, it should be noted that unless otherwise clearly defined and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense. For example, it can be a fixed connection or a detachable connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the internal communication of two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.

[0025] Embodiment 1

[0026] As Figure 1 - Figure 2 shown, to solve the above problems, the present utility model provides a detection card for anti-hemolysis interference, including:

[0027] Bottom plate 8, at least a part of the upper surface of the bottom plate 8 is provided with a filtration chromatography structure, and the filtration chromatography structure includes a diffusion layer 1, a blood filtration layer 2, a chromatography pad 3, and a support layer 4 that are sequentially overlapped from top to bottom on the upper surface of the bottom plate; the support layer 4 has a notch at a position corresponding to the reaction hole 9 of the bottom plate 8, the notch is located below the chromatography pad 3 and is completely covered by the chromatography pad 3, and a reaction layer 10 and a treatment layer 11 are adhesively bonded to the notch from top to bottom in sequence.

[0028] Absorbent pad 7, at least a part of the absorbent pad 7 is adhesively bonded above the support layer 4 and at least a part is adhesively bonded above the chromatography pad 3.

[0029] Further, the diffusion layer 1 is a mesh screen with a mesh density of 50 - 1000 meshes, and the material of the diffusion layer 1 is one of nylon film, polyester film, polyethylene film, and paper material.

[0030] Further, the material of the blood filtration layer 2 is one of polyester film, polyethersulfone film, and cellulose acetate filter membrane.

[0031] Further, the material of the chromatography pad 3 is a nitrocellulose membrane, the chromatography pad 3 is provided with a retention part 5, the retention part 5 divides the chromatography pad 3 into a first chromatography sub - part and a second chromatography sub - part, the diffusion layer 1 and the blood filtration layer 2 only cover the upper part of the first chromatography sub - part, and the absorbent pad 7 only covers a partial area above the second chromatography sub - part; the surface of the retention part 5 is coated with a coating solution containing hemoglobin antibody, and the material of the retention part 5 is a nitrocellulose membrane.

[0032] Further, the material of the support layer 4 is one of silica gel and polytetrafluoroethylene.

[0033] Further, the material of the treatment layer 11 is one of polyester film, polyethersulfone film, and glass fiber membrane.

[0034] Further, the material of the reaction layer 10 is one of polyester film, polyethersulfone film, and glass fiber membrane.

[0035] Further, a single - color light - shielding plate 6 is arranged above the absorbent pad 7, and a part of the light - shielding plate 6 is adhesively bonded above the absorbent pad 7 and another part is adhesively bonded above the chromatography pad 3.

[0036] Further, the color of the light - shielding plate 6 is black, and the material of the light - shielding plate 6 is one of polyvinyl chloride film, polyethylene film, and polyester film.

[0037] Further, the transmittance of the light - shielding plate 6 is between 0 and 55%.

[0038] The working principle of the present utility model:

[0039] Drop a whole blood sample onto the diffusion layer 1. After the sample diffuses on the diffusion layer 1, it seeps into the blood filtering layer 2, and the blood cells in the sample are filtered out by the blood filtering layer 2. Subsequently, the sample continues to penetrate under the action of gravity into the first chromatography section of the chromatography pad 3, and enters the second chromatography section through the interception section 5 under the action of the water absorption pad 7. During this process, the interception section 5 can intercept hemoglobin in the sample and bind to the hemoglobin through the hemoglobin antibody on its surface to eliminate hemoglobin. Then, it successively enters the reaction layer 10 and the treatment layer 11 from the second chromatography section. Finally, after the treatment layer 11 removes or activates interfering substances in the sample, the sample settles to the reaction layer 10 for a color reaction. Under the action of the black light-shielding plate 6, the background of the reaction layer 10 remains monotonously consistent in color, making the detection more accurate.

[0040] Although the present utility model has been disclosed above with preferred embodiments, it is not intended to limit the present utility model. Anyone familiar with this technology can make various modifications and alterations without departing from the spirit and scope of the present utility model. Therefore, the protection scope of the present utility model should be defined by the claims.

Claims

1. A test card for resisting hemolysis interference, characterized in that: include: A bottom plate, wherein at least a portion of the upper surface of the bottom plate is provided with a filtration chromatography structure, wherein the filtration chromatography structure comprises a diffusion layer, a blood filter layer, a chromatography pad, and a support layer which are sequentially overlapped and arranged on the upper surface of the bottom plate from top to bottom; the support layer has a gap at a position corresponding to the reaction hole of the bottom plate, the gap is located below the chromatography pad and is completely covered by the chromatography pad, and the gap is sequentially bonded with a reaction layer and a treatment layer from top to bottom; An absorbent pad is at least partially bonded to the support layer and at least partially bonded to the chromatography pad.

2. The anti-hemolysis interference detection card according to claim 1, characterized in that: The diffusion layer is a gauze with a mesh density of 50-1000 meshes, and the material of the diffusion layer is one of nylon film, polyester film, polyethylene film, and paper material.

3. The anti-hemolysis interference detection card according to claim 1, characterized in that: The material of the blood filter layer is one of polyester membrane, polyethersulfone membrane and cellulose acetate filter membrane.

4. The anti-hemolysis interference detection card according to claim 1, characterized in that: The material of the chromatography pad is nitrocellulose membrane, and the chromatography pad is provided with a retention part, which separates the chromatography pad into a first chromatography section and a second chromatography section, the diffusion layer and the blood filtration layer only cover the upper part of the first chromatography section, and the absorbent pad only covers a partial area above the second chromatography section; the surface of the retention part is coated with a coating solution containing hemoglobin antibodies, and the material of the retention part is nitrocellulose membrane.

5. The anti-hemolysis interference detection card according to claim 1, characterized in that: The material of the support layer is one of silica gel and polytetrafluoroethylene.

6. The anti-hemolysis interference detection card according to claim 1, characterized in that: The material of the treatment layer is one of polyester film, polyethersulfone film and glass fiber film.

7. The anti-hemolysis interference detection card according to claim 1, characterized in that: The material of the reaction layer is one of polyester film, polyethersulfone film and glass fiber film.

8. The anti-hemolysis interference detection card according to claim 4, characterized in that: A monochrome shading plate is arranged above the water absorbing pad, a part of the shading plate is bonded to the top of the water absorbing pad, and another part of the shading plate is bonded to the top of the chromatography pad.

9. The anti-hemolysis interference detection card according to claim 8, characterized in that: The color of the shading plate is black, and the material of the shading plate is one of polyvinyl chloride film, polyethylene film, and polyester film.

10. The anti-hemolysis interference detection card according to claim 9, characterized in that: The transmittance of the shading plate is between 0% and 55%.

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