Cold precipitation device for phosphatidylserine production

Abstract

The utility model relates to the technical field of phosphatidylserine production, in particular to a phosphatidylserine production cold sinking device which comprises a cold sinking barrel, an inner container is fixedly connected to the lower portion in the cold sinking barrel, discharge valves are fixedly connected to the middle of the bottom of the inner container and the middle of the bottom of the cold sinking barrel, and a hollow copper pipe is arranged on the outer side of the inner container. The input end of the hollow copper pipe is fixedly connected with a water injection pump, the output end of the hollow copper pipe is fixedly connected with a drainage pump, and a PH sensor is fixedly connected in the middle of the right of the cold sinking barrel and the inner container. Through design cooperation, the device can monitor the PH value of phosphatidylserine liquid in cold precipitation in the inner container in real time, and when the PH value exceeds a set value, the controller controls the corresponding metering pump to inject acid liquid or alkaline liquid stored in the storage barrel into the inner container, so that the phosphatidylserine liquid in cold precipitation in the inner container is cooled. The motor is matched to drive the stirring blades to stir and mix the phosphatidylserine and the acidic or alkaline liquid in the inner container, so that the condition of local over-acidity or over-alkalinity is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of phosphatidylserine production, in particular to a cold precipitation device for phosphatidylserine production. Background Art

[0002] Phosphatidylserine is a kind of phospholipid substance mainly existing inside the cell membrane. It is an important component of the cell membrane structure, plays an important role in the function and signal transmission of the cell membrane, can be used as part of a drug delivery system, is used to prepare drug carriers such as liposomes, helps drugs cross the cell membrane, and improves the bioavailability and targeting of drugs.

[0003] The patent specification with the publication number of CN212998559U discloses a cold precipitation device for phosphatidylserine production, including a workbench. A cold precipitation structure is installed on the upper wall surface of the workbench, a control structure is installed on the upper wall surface of the workbench, a power supply structure is installed on the rear wall surface of the workbench, and a support structure is installed on the lower wall surface of the workbench; the cold precipitation structure includes: a base, a protective sleeve and a cooling inner tank; the base is installed on the upper wall surface of the workbench, the protective sleeve is provided with an inner hole and is installed on the upper wall surface of the base, and the cooling inner tank is installed in the inner hole opened by the protective sleeve. The utility model relates to the technical field of phosphatidylserine cold precipitation. The cold precipitation device for phosphatidylserine production has a simple structure, low production cost, is convenient for disassembly and assembly, is suitable for maintenance and cleaning, and has simple operation. Operators do not need too high professional skills and can operate according to the instruction manual. The cold precipitation device for phosphatidylserine production has a cooling function, greatly shortens the precipitation time, improves the purity of phosphatidylserine, and greatly improves the working efficiency of the device.

[0004] However, it is found that the above-mentioned cold precipitation device for phosphatidylserine production has the following problems in the implementation of related technologies: When phosphatidylserine is subjected to cold precipitation, temperature and pH value are two key parameters, which have a significant impact on the arrangement, aggregation and finally formed structure of phospholipid molecules. When the above structure performs cold precipitation on phosphatidylserine, the phosphatidylserine is cold-precipitated through the cooling inner tank, and the pH value inside it may need to be manually tested every once in a while, and the corresponding acidic or alkaline solution is manually added according to the test results, which increases the labor intensity of operators to a certain extent. In view of this, a cold precipitation device for phosphatidylserine production is provided to overcome the above defects. Summary of the Utility Model

[0005] The purpose of the utility model is to solve the defects existing in the prior art and propose a cold precipitation device for phosphatidylserine production.

[0006] To achieve the above object, the present utility model adopts the following technical solution: A cold precipitation device for phosphatidylserine production, comprising a cold precipitation barrel, wherein an inner tank is fixedly connected to the lower part inside the cold precipitation barrel, discharge valves are fixedly connected to the middle of the bottom of both the inner tank and the cold precipitation barrel, a hollow copper pipe is arranged outside the inner tank, a water injection pump is fixedly connected to the input end of the hollow copper pipe, a drainage pump is fixedly connected to the output end of the hollow copper pipe, a PH sensor is fixedly connected to the middle inside the right side between the cold precipitation barrel and the inner tank, a top cover is slidably connected to the outer side above the cold precipitation barrel, a controller is fixedly connected to the middle of the front side of the top of the top cover, a motor is fixedly connected to the middle of the top of the top cover, a stirring blade is fixedly connected to the outer side below the output end of the motor, metering pumps are fixedly connected to the inside of the left and right sides above the top cover, and a storage barrel is fixedly connected to the input end of the metering pump.

[0007] As a further description of the above technical solution: The right side inside the lower part of the cold precipitation barrel is fixedly connected to the outer side of the hollow copper pipe near the water injection pump and the drainage pump, and the outer side above the inner tank is in contact with the inner side below the top cover, which can reduce the number of times for operators to manually detect the PH value of phosphatidylserine to a certain extent and reduce the labor intensity of operators.

[0008] As a further description of the above technical solution: The PH sensor is electrically connected to the controller, and the controller is electrically connected to the two metering pumps. The metering pump on the left side of the top of the top cover transports acidic liquid, and the metering pump on the right side of the top of the top cover transports alkaline liquid. If the result detected by the PH sensor exceeds 7.5, the controller controls the metering pump on the left side of the top of the top cover to start, and if the result detected by the PH sensor is lower than 6.5, the controller controls the metering pump on the right side of the top of the top cover to start. It can monitor the PH value of the phosphatidylserine liquid being cold-precipitated in the inner tank in real time. When the PH value exceeds the set value, the controller controls the corresponding metering pump to inject the acidic liquid or alkaline liquid stored in the storage barrel into the inner tank.

[0009] As a further description of the above technical solution: The controller is electrically connected to the motor, and when the controller controls the metering pump on the left or right side of the top of the top cover to start, the motor starts at the same time. When the controller controls the metering pump on the left or right side of the top of the top cover to close, the motor closes after 10 seconds. It can drive the stirring blade to stir and mix the phosphatidylserine and acidic or alkaline liquid in the inner tank with the motor when the acidic or alkaline solution is injected into the inner tank, avoiding the situation of local over-acidity or over-alkalinity.

[0010] As a further description of the above technical solution: The hollow copper tube is arranged in a circular shape as a whole and closely attached to the outside of the inner tank. The rear top end of the hollow copper tube is the input end, and the front top end of the hollow copper tube is the output end. Cooling water at a temperature of five degrees Celsius is injected and conveyed into the hollow copper tube through a water injection pump. The cooling water flowing inside the hollow copper tube surrounding the outside of the inner tank can reduce the temperature of phosphatidylserine in the inner tank, enabling the phosphatidylserine in the inner tank to continue to undergo cold precipitation.

[0011] As a further description of the above technical solution: Circular through holes are provided at the middle positions on the left and right sides of the top of the top cover and in the middle of the inside of the top cover. The diameter of the circular through holes at the middle positions on the left and right sides of the top of the top cover matches the cross-sectional diameter of the output end of the metering pump, and the diameter of the circular through hole in the middle of the inside of the top cover matches the cross-sectional diameter of the output end of the motor, allowing the output ends of both the motor and the metering pump to pass through the top cover. The output end of the motor passes through the top cover to rotate and stir the phosphatidylserine in the inner tank with the stirring blade, while the output end of the metering pump passes through the top cover to inject acidic or alkaline liquid into the phosphatidylserine.

[0012] As a further description of the above technical solution: A cover plate is clamped on the top of the storage barrel. A hollow shell is fixedly connected to the top of the cover plate. A vibration motor is fixedly connected to the lower part inside the hollow shell. Guide rods are fixedly connected to the left and right sides at the bottom of the hollow shell. The left and right sides inside the cover plate are fixedly connected to the outer sides above the guide rods, enabling the vibration generated by the vibration motor to be transmitted to the acidic or alkaline liquid through the guide rods inserted into the acidic or alkaline liquid, so that the high-frequency vibration of the guide rods is transmitted to the acidic or alkaline liquid, preventing sediment from forming inside the acidic or alkaline liquid after long-term stillness.

[0013] As a further description of the above technical solution: The length of the guide rod is two-thirds of the inner depth of the storage barrel. Holes are provided at the middle positions on the left and right sides inside the cover plate, and the diameter of the holes matches the cross-sectional diameter of the guide rod. The bottom end of the guide rod can be suspended inside the storage barrel near the bottom but not in contact with the bottom, allowing the high-frequency vibration generated by the guide rod to be transmitted to all the solutions inside the storage barrel, preventing the solution at the bottom from not being vibrated by the vibration due to the too short guide rod.

[0014] The utility model has the following beneficial effects:

[0015] The phosphatidylserine production cold precipitation device designed by the utility model, through design cooperation, enables the device to monitor the pH value of the phosphatidylserine liquid undergoing cold precipitation in the inner tank in real time. When the pH value exceeds the set value, the corresponding metering pump is controlled by the controller to inject the acidic liquid or alkaline liquid stored in the storage tank into the inner tank, and the motor drives the stirring blade to stir and mix the phosphatidylserine and the acidic or alkaline liquid in the inner tank, avoiding the situation of local over-acidity or over-alkalinity. The motor is also turned off ten seconds after the metering pump stops delivering the acidic or alkaline liquid. With the cooling water flowing inside the hollow copper pipe surrounding the outer side of the inner tank, the phosphatidylserine in the inner tank continues to undergo cold precipitation, reducing the number of times the operator manually detects the pH value of phosphatidylserine to a certain extent and reducing the labor intensity of the operator.

[0016] The phosphatidylserine production cold precipitation device designed by the utility model, through design cooperation, enables the device to transmit the vibration sensation generated by the vibration motor through the guide rod inserted into the acidic or alkaline liquid, and the high-frequency vibration of the guide rod is transmitted to the acidic or alkaline liquid to keep the solute inside the liquid evenly distributed. It can effectively avoid the situation that after the acidic or alkaline liquid stands still for a long time, the precipitate generated inside it enters the metering pump with the acidic or alkaline liquid, affecting the delivery of the acidic or alkaline liquid by the metering pump, and extending the maintenance interval of the metering pump to a certain extent. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is the overall structural schematic diagram of the utility model;

[0018] Figure 2 is the three-dimensional structural schematic diagram of the top cover of the utility model longitudinally flipped 180 degrees;

[0019] Figure 3 is the three-dimensional longitudinal sectional structural schematic diagram of the cold precipitation barrel and the inner tank of the utility model;

[0020] Figure 4 is the three-dimensional longitudinal sectional structural schematic diagram of the storage tank of the utility model.

[0021] LEGEND DESCRIPTION:

[0022] 1. Cold precipitation barrel; 2. Top cover; 3. pH sensor; 4. Metering pump; 5. Storage tank; 6. Cover plate; 7. Hollow shell; 8. Motor; 9. Controller; 10. Water injection pump; 11. Drainage pump; 12. Stirring blade; 13. Inner tank; 14. Discharge valve; 15. Hollow copper pipe; 16. Vibration motor; 17. Guide rod. DETAILED IMPLEMENTATION MANNER

[0023] Refer to Figures 1 - 4, a phosphatidylserine production cold precipitation device provided by the utility model includes a cold precipitation barrel 1. An inner tank 13 is welded below the interior of the cold precipitation barrel 1. A discharge valve 14 is fixed by bolts in the middle of the bottoms of both the inner tank 13 and the cold precipitation barrel 1. A hollow copper pipe 15 is arranged outside the inner tank 13. The input end of the hollow copper pipe 15 is fixed by bolts to a water injection pump 10, and the output end of the hollow copper pipe 15 is fixed by bolts to a drainage pump 11. A PH sensor 3 is fixed by bolts in the middle of the interior on the right side of the cold precipitation barrel 1 and the inner tank 13. The upper outer side of the cold precipitation barrel 1 is contacted by a top cover 2. A controller 9 is fixed by bolts in the middle of the front side of the top of the top cover 2. A motor 8 is fixed by bolts in the middle of the top of the top cover 2. The output shaft of the output end of the motor 8 penetrates through the interior of a stirring blade 12 and is welded to it below the outer side. The output ends of metering pumps 4 penetrate through the interior on the left and right sides above the top cover 2 and are fixed by bolts. The input ends of the metering pumps 4 penetrate through the lower part inside a storage barrel 5 and are fixed by bolts. The interior on the right side below the cold precipitation barrel 1 is fixedly connected to the outer side of one end of the hollow copper pipe 15 close to the water injection pump 10 and the drainage pump 11. The upper outer side of the inner tank 13 contacts the lower inner side of the top cover 2, which can reduce the number of times for operators to manually detect the PH value of phosphatidylserine to a certain extent and reduce the labor intensity of operators.

[0024] As a further implementation of the above technical solution: The PH sensor 3 is electrically connected to the controller 9, and the controller 9 is electrically connected to the two metering pumps 4. The metering pump 4 on the left side of the top of the top cover 2 transports acidic liquid, and the metering pump 4 on the right side of the top of the top cover 2 transports alkaline liquid. If the result detected by the PH sensor 3 exceeds 7.5, the controller 9 controls the metering pump 4 on the left side of the top of the top cover 2 to start. If the result detected by the PH sensor 3 is lower than 6.5, the controller 9 controls the metering pump 4 on the right side of the top of the top cover 2 to start. The PH value of the phosphatidylserine liquid being cold-precipitated in the inner tank 13 can be monitored in real time. When the PH value exceeds the set value, the corresponding metering pump 4 is controlled by the controller 9 to inject the acidic liquid or alkaline liquid stored in the storage barrel 5 into the inner tank 13.

[0025] As a further implementation of the above technical solution: The controller 9 is electrically connected to the motor 8, and when the controller 9 controls the metering pump 4 on the left or right side of the top of the top cover 2 to start, the motor 8 starts simultaneously. When the controller 9 controls the metering pump 4 on the left or right side of the top of the top cover 2 to close, the motor 8 closes after 10 seconds. When the acidic or alkaline solution is injected into the inner tank 13, it can cooperate with the motor 8 to drive the stirring blade 12 to stir and mix the phosphatidylserine and the acidic or alkaline liquid in the inner tank 13 to avoid the situation of local over-acidity or over-alkalinity.

[0026] As a further implementation of the above technical solution: The hollow copper tube 15 is arranged in a whole ring shape and closely attached to the outer side of the inner tank 13. The rear top end of the hollow copper tube 15 is the input end, and the front top end of the hollow copper tube 15 is the output end. Cooling water at a temperature of five degrees Celsius is injected and conveyed into the hollow copper tube 15 through a water injection pump 10. The cooling water flowing inside the hollow copper tube 15 surrounding the outer side of the inner tank 13 can reduce the temperature of phosphatidylserine in the inner tank 13, enabling the phosphatidylserine in the inner tank 13 to continue to undergo cold precipitation.

[0027] As a further implementation of the above technical solution: Circular through holes are provided at the middle positions on the left and right sides of the top of the top cover 2 and in the middle of the inside of the top cover 2. The diameter of the circular through holes at the middle positions on the left and right sides of the top of the top cover 2 matches the cross-sectional diameter of the output end of the metering pump 4, and the diameter of the circular through hole in the middle of the inside of the top cover 2 matches the cross-sectional diameter of the output end of the motor 8, allowing the output ends of both the motor 8 and the metering pump 4 to pass through the top cover 2. The output end of the motor 8 passes through the top cover 2 to rotate and stir the phosphatidylserine in the inner tank 13 with the stirring blade 12, while the output end of the metering pump 4 passes through the top cover 2 to inject acidic or alkaline liquid into the phosphatidylserine.

[0028] As a further implementation of the above technical solution: A cover plate 6 is provided at the top of the storage bucket 5, and a hollow shell 7 is welded to the top of the cover plate 6. A vibration motor 16 is fixed to the lower part inside the hollow shell 7 by bolts. Guide rods 17 are welded to the left and right sides at the bottom of the hollow shell 7, and the left and right sides inside the cover plate 6 are fixedly connected to the outer sides above the guide rods 17. The vibration generated by the vibration motor 16 can be transmitted to the acidic or alkaline liquid through the guide rods 17 inserted into the acidic or alkaline liquid, enabling the high-frequency vibration of the guide rods 17 to be transmitted to the acidic or alkaline liquid, and avoiding the formation of precipitates inside the acidic or alkaline liquid after long-term stillness.

[0029] As a further implementation of the above technical solution: The length of the guide rod 17 is two-thirds of the inner depth of the storage bucket 5. Holes are provided at the middle positions on the left and right sides inside the cover plate 6, and the diameter of the holes matches the cross-sectional diameter of the guide rod 17. The bottom end of the guide rod 17 can be suspended inside the storage bucket 5 near the bottom but not in contact with the bottom, allowing the high-frequency vibration generated by the guide rod 17 to be transmitted to all the solutions inside the storage bucket 5, and avoiding the solution at the bottom not being vibrated by the vibration due to the too short length of the guide rod 17.

[0030] Among them, the PH sensor 3, the controller 9, and the metering pump 4 used in this specification are all common electronic structures on the market. The specific model of the PH sensor 3 is: LH - BODK81 - BOD, the specific model of the controller 9 is the PLC controller 9 of Siemens S7 - 1200, and the specific model of the metering pump 4 is the quantitative vane pump yb - 40 / 6.

[0031] Working principle:

[0032] When using the present utility model, acidic and alkaline solutions are injected into the hollow shell 7 above the top cover 2. The acidic solution is injected into the left hollow shell 7 above the top cover 2, and the alkaline solution is injected into the right hollow shell 7 above the top cover 2. Subsequently, the injection water pump 10 and the drainage pump 11 are turned on. The injection water pump 10 extracts cooling water at a temperature of five degrees Celsius and injects it into the hollow copper tube 15. After circulating in the annular hollow copper tube 15, it is discharged by the drainage pump 11. After the preparation work is completed, phosphatidylserine is poured into the inner tank 13 inside the cold sedimentation barrel 1. Subsequently, the top cover 2 is covered, and the stirring blade 12 below the top cover 2 is inserted into the phosphatidylserine. The PH sensor 3 is turned on, and the PH sensor 3 monitors the phosphatidylserine in the inner tank 13 in real time. In cooperation with the cooling water flowing inside the hollow copper tube 15, the temperature of the phosphatidylserine in the inner tank 13 will also decrease accordingly. At this time, the PH value of the phosphatidylserine changes due to the temperature drop. When the PH value exceeds 7.5, it means that the phosphatidylserine has a high alkalinity. Then, the controller 9 controls the metering pump 4 on the left side of the top cover 2 to inject the acidic liquid stored in the storage tank 5 into the inner tank 13. When the PH value is lower than 6.5, it means that the phosphatidylserine has a high acidity. Then, the controller 9 controls the metering pump 4 on the right side of the top cover 2 to inject the alkaline liquid stored in the storage tank 5 into the inner tank 13. At the same time, the motor 8 is turned on, and the motor 8 drives the stirring blade 12 to stir and mix the phosphatidylserine and the acidic or alkaline liquid in the inner tank 13 to avoid local over-acidity or over-alkalinity. The motor 8 is also turned off ten seconds after the metering pump 4 stops delivering the acidic or alkaline liquid, and the phosphatidylserine continues to be cold-settled. The upper parts of the two storage tanks 5 are covered with a cover plate 6, and the guide rod 17 below the cover plate 6 is inserted into the acidic and alkaline solutions. The vibration motor 16 inside the hollow shell 7 is periodically turned on. The vibration generated by the vibration motor 16 is transmitted to the acidic or alkaline liquid through the guide rod 17 inserted into the acidic or alkaline liquid, so that the high-frequency vibration of the guide rod 17 is transmitted to the acidic or alkaline liquid, avoiding the generation of precipitates inside the acidic or alkaline liquid after long-term stillness, and keeping the solutes inside the liquid evenly distributed through the high-frequency vibration of the guide rod 17 every once in a while. When the phosphatidylserine in the inner tank 13 is cold-settled, the discharge valve 14 is opened to discharge the phosphatidylserine from the inner tank 13.

[0033] Finally, it should be noted that the above are only the preferred embodiments of the present utility model and are not used to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present utility model shall be included in the protection scope of the present utility model.

Claims

1. A cold sinking device for producing phosphatidylserine, comprising a cold sinking barrel (1), characterized in that: An inner tank (13) is fixedly connected to the lower part of the cold sink barrel (1), and a discharge valve (14) is fixedly connected to the middle of the inner tank (13) and the bottom of the cold sink barrel (1). A hollow copper tube (15) is arranged on the outer side of the inner tank (13), and a water injection pump (10) is fixedly connected to the input end of the hollow copper tube (15), and a drainage pump (11) is fixedly connected to the output end of the hollow copper tube (15). A pH sensor (3) is fixedly connected to the middle of the right side of the cold sink barrel (1) and the inner tank (13). A top cover (2) is slidably connected to the outer side of the upper part of the cold sink barrel (1), and a controller (9) is fixedly connected to the middle of the top front side of the top cover (2). A motor (8) is fixedly connected to the middle of the top of the top cover (2), and a stirring blade (12) is fixedly connected to the outer side below the output end of the motor (8). A metering pump (4) is fixedly connected to the left and right sides of the upper part of the top cover (2), and a storage barrel (5) is fixedly connected to the input end of the metering pump (4).

2. A phosphatidylserine production cold sink device according to claim 1, characterized in that: The inner right side of the lower side of the cold sink barrel (1) is fixedly connected to the outer side of one end of the hollow copper tube (15) close to the water injection pump (10) and the drainage pump (11), and the upper outer side of the inner tank (13) is in contact with the lower inner side of the top cover (2).

3. A phosphatidylserine production cold sink device according to claim 1, characterized in that: The pH sensor (3) is electrically connected to the controller (9), and the controller (9) is electrically connected to two metering pumps (4). The metering pump (4) on the left side of the top cover (2) delivers an acidic liquid, and the metering pump (4) on the right side of the top cover (2) delivers an alkaline liquid. If the result detected by the pH sensor (3) exceeds 7.5, the metering pump (4) on the left side of the top cover (2) is controlled by the controller (9) to be turned on. If the result detected by the pH sensor (3) is lower than 6.5, the metering pump (4) on the right side of the top cover (2) is controlled by the controller (9) to be turned on.

4. A phosphatidylserine production cold sink device according to claim 1, characterized in that: The controller (9) is electrically connected to the motor (8), and when the metering pump (4) on the left or right side of the top of the top cover (2) is turned on, the motor (8) is turned on together with the controller (9), and when the metering pump (4) on the left or right side of the top of the top cover (2) is turned off, the motor (8) is turned off ten seconds after the controller (9) controls the metering pump (4) on the left or right side of the top of the top cover (2) to be turned off.

5. A phosphatidylserine production cold sink device according to claim 1, characterized in that: The hollow copper tube (15) is arranged in a whole ring shape and is closely attached to the outer side of the inner tank (13). The rear top end of the hollow copper tube (15) is the input end, and the front top end of the hollow copper tube (15) is the output end. The inside of the hollow copper tube (15) is injected and transported with cooling water at a temperature of five degrees Celsius through a water injection pump (10).

6. A phosphatidylserine production cold sink device according to claim 1, characterized in that: Circular through holes are provided at the middle positions of the left and right sides of the top of the top cover (2) and at the middle of the interior of the top cover (2); the diameter of the circular through holes at the middle positions of the left and right sides of the top of the top cover (2) matches the cross-sectional diameter of the output end of the metering pump (4), and the diameter of the circular through hole in the middle of the interior of the top cover (2) matches the cross-sectional diameter of the output end of the motor (8).

7. A phosphatidylserine production cold sink device according to claim 1, characterized in that: A cover plate (6) is engaged with the top of the storage barrel (5), a hollow shell (7) is fixedly connected to the top of the cover plate (6), a vibration motor (16) is fixedly connected to the lower part of the hollow shell (7), guide rods (17) are fixedly connected to the left and right sides of the bottom of the hollow shell (7), and the left and right sides of the cover plate (6) are fixedly connected to the upper outer side of the guide rod (17).

8. A phosphatidylserine production cold sink device according to claim 7, characterized in that: The length of the guide rod (17) is two-thirds of the inner depth of the storage barrel (5), and holes are provided in the middle of the left and right sides of the cover plate (6), and the diameter of the holes matches the cross-sectional diameter of the guide rod (17).

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