Anti-suck-back consumable of pipette and pipette

Abstract

The pipette anti-suck-back consumable comprises a hollow rotary consumable body, the consumable body sequentially comprises a detachable connection part and a filter element installation part from top to bottom, the detachable connection part is used for being detachably and fixedly connected with a negative pressure pipe of the pipette, and the filter element installation part is used for being detachably and fixedly connected with a negative pressure pipe of the pipette. A filter element A is fixedly arranged in a cavity of the filter element mounting part and changes color when encountering water. In the using process, if the filter element A does not change color, only the gun head without the filter element needs to be replaced; if the color of the filter element A is changed, it is indicated that suck-back occurs in the pipetting operation process, and a new pipetting gun suck-back prevention consumable needs to be replaced. The technical scheme has a great economical advantage.

Description

Technical Field

[0001] The present invention belongs to the technical field of pipettes, and particularly relates to an anti-backflow consumable for a pipette and a pipette. Background Art

[0002] A pipettor is an indispensable instrument in biological research, and a pipette gun is a type of pipettor. When using a pipette gun to aspirate liquid, generally, the pipette gun is used in combination with a pipette tip. The pipette tip is inserted at the lower end of the negative pressure tube of the pipette gun. After use, the pipette tip is pushed off by pressing the pipette tip ejection button on the pipette gun to separate the pipette gun from the pipette tip, as Figures 1 - 4 shown.

[0003] In order to prevent backflow during sample addition, currently, a pipette tip equipped with a filter element (as shown in the Chinese invention patent publication text with the publication number CN102921483A) is generally selected. The filter element prevents the liquid from being back-sucked into the negative pressure tube, thereby preventing the contamination of the pipette gun body.

[0004] The pipette tip belongs to a consumable, generally disposable and single-use. In terms of cost, the pipette tip with a filter element is about 70% more expensive than the pipette tip without a filter element. How to reduce the cost of using the pipette tip on the premise of preventing the liquid from being back-sucked into the pipette gun is the design concept of the technical solution of the present invention. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to make up for the deficiencies of the prior art and provide an anti-backflow consumable for a pipette and a pipette.

[0006] To solve the above technical problem, the technical solution of the present invention is as follows:

[0007] An anti-backflow consumable for a pipette gun includes a hollow rotary consumable body. The consumable body sequentially includes a detachable connection part and a filter element installation part connected integrally from top to bottom. The detachable connection part is used for detachably and fixedly connecting with the negative pressure tube of the pipette gun. The outer diameter of the filter element installation part is R, where r0 < R < R0. Here, r0 is the inner diameter of the negative pressure tube of the pipette gun, and R0 is the outer diameter of the negative pressure tube of the pipette gun. The height H of the filter element installation part is H ≤ 15 mm. A filter element A is fixedly arranged in the cavity of the filter element installation part, and the material on the outer surface of the filter element A is a material that changes color when encountering water.

[0008] Further, the detachable fixed connection method between the detachable connection part of the consumable body and the negative pressure tube of the pipette gun is insertion connection. A soft rubber layer is arranged on the outer circumference of the detachable connection part, and the soft rubber layer is in interference fit with the inner wall of the negative pressure tube. A guiding part is arranged above the detachable connection part, and the guiding part is a hollow frustum shape with a smaller upper part and a larger lower part.

[0009] Further, the detachable fixed connection method between the detachable connection part of the consumable body and the negative pressure tube of the pipette gun is threaded connection, and an external thread A is arranged on the outer circumference of the detachable connection part.

[0010] A pipette gun includes a negative pressure tube and a gun head removing tube sleeved outside the negative pressure tube. When the gun head removing tube is in the upper position, the lower end face of the gun head removing tube is above the lower end face of the negative pressure tube; when the gun head removing tube is in the lower position, the lower end face of the gun head removing tube is not higher than the lower end face of the negative pressure tube. The lower end of the negative pressure tube is used to connect with a gun head and is used for matching use with the anti-backflow consumable of the pipette gun as described above. An internal thread A is provided at the lower end of the negative pressure tube, and the internal thread A is used for screwing connection with an external thread A on the anti-backflow consumable of the pipette gun.

[0011] An anti-backflow consumable for a pipette gun includes a hollow rotary consumable body. The consumable body successively includes a disassembling and connecting part and a filter element installation part that are integrally connected from top to bottom. The disassembling and connecting part is used for fixed connection with the negative pressure tube of the pipette gun through threads. The outer diameter of the filter element installation part is R, and R = R0, where R0 is the outer diameter of the negative pressure tube of the pipette gun. The height H of the filter element installation part is ≥50 mm. A filter element A is fixedly provided at the top in the cavity of the filter element installation part, and the material on the outer surface of the filter element A is a material that changes color when encountering water.

[0012] A pipette gun includes a negative pressure tube and a gun head removing tube sleeved outside the negative pressure tube. When the gun head removing tube is in the upper position, the lower end face of the gun head removing tube is above the lower end face of the filter element installation part of the anti-backflow consumable of the pipette gun; when the gun head removing tube is in the lower position, the lower end face of the gun head removing tube is not higher than the lower end face of the filter element installation part of the anti-backflow consumable of the pipette gun. It is used for matching use with the anti-backflow consumable of the pipette gun as described above. A thread B is provided at the lower end of the negative pressure tube, and the thread B is used for screwing connection with the anti-backflow consumable of the pipette gun. The bottom end of the anti-backflow consumable of the pipette gun is used to connect with a gun head. It further includes a hollow rotary gun head removing tube extension. The gun head removing tube extension includes a connecting head and a punching tube that are integrally connected. The connecting head is fixedly connected with the gun head removing tube. The inner diameter of the punching tube is ry, and ry = rx, where rx is the inner diameter of the gun head removing tube. When the gun head removing tube is in the upper position, the lower end face of the punching tube is above the lower end face of the filter element installation part of the anti-backflow consumable of the pipette gun; when the gun head removing tube is in the lower position, the lower end face of the punching tube is not higher than the lower end face of the filter element installation part of the anti-backflow consumable of the pipette gun.

[0013] A pipette gun includes a negative pressure tube and a gun head removing tube sleeved outside the negative pressure tube. It is used for matching use with the anti-backflow consumable of the pipette gun as described above. A thread C is provided at the lower end of the negative pressure tube, and the thread C is used for screwing connection with the anti-backflow consumable of the pipette gun. The bottom end of the anti-backflow consumable of the pipette gun is used to connect with a gun head. When the gun head removing tube is in the upper position, the lower end face of the gun head removing tube is above the lower end face of the filter element installation part of the anti-backflow consumable of the pipette gun; when the gun head removing tube is in the lower position, the lower end face of the gun head removing tube is not higher than the lower end face of the filter element installation part of the anti-backflow consumable of the pipette gun.

[0014] The beneficial effects that can be achieved by the present invention are:

[0015] 1. During the use of the pipette anti-backflow consumable, if the filter element A in the pipette anti-backflow consumable does not change color, it indicates that no backflow phenomenon occurs during the pipetting operation, and only the filterless pipette tip needs to be replaced; if it is found that the filter element A in the pipette anti-backflow consumable changes color, it indicates that backflow occurs during the pipetting operation, and a new pipette anti-backflow consumable needs to be replaced. The cost of the pipette anti-backflow consumable is equivalent to that of the filter-tipped pipette tip. As long as the occurrence probability of the backflow phenomenon is lower than 33%, the economic advantage of this technical solution can be reflected, and in actual operation, the occurrence probability of the backflow phenomenon is lower than 10%.

[0016] 2. Further, on the basis of setting the pipette anti-backflow consumable, the pipette anti-backflow consumable is lengthened, which further effectively prevents the backflow material from entering the pipette and ensures the cleanliness of the pipette. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is a schematic diagram of the matching use of a pipette and a pipette tip in the prior art.

[0018] Figure 2 is a schematic diagram of the matching use of a pipette and a filter-tipped pipette tip in the prior art.

[0019] Figure 3 is Figure 2 an enlarged view of part A in

[0020] Figure 4 is a schematic diagram of the state where the filter-tipped pipette tip is removed from the pipette in the prior art.

[0021] Figure 5 is a schematic diagram of the matching use of the anti-backflow consumable A, the filterless pipette tip and the pipette in Embodiment 1 of the present invention.

[0022] Figure 6 is Figure 5 an enlarged view of part B in

[0023] Figure 7 is a schematic diagram of the state where the filterless pipette tip is removed from the pipette in Embodiment 1 of the present invention.

[0024] Figure 8 is a schematic diagram of the matching use of the anti-backflow consumable B, the filterless pipette tip and the pipette in Embodiment 2 of the present invention.

[0025] Figure 9 is Figure 8 an enlarged view of part E in

[0026] Figure 10 is a schematic diagram of the state where the filterless pipette tip is removed from the pipette in Embodiment 2 of the present invention.

[0027] Figure 11 It is a schematic diagram of the matching use of the anti-backflow consumable C, the filterless pipette tip, the pipette tip removal tube extension, and the pipette in Embodiment 3 of the present invention.

[0028] Figure 12 is Figure 11 an enlarged view of part C in

[0029] Figure 13 It is a schematic diagram of the state where the filterless pipette tip is removed from the pipette in Embodiment 3 of the present invention.

[0030] Figure 14 It is a schematic diagram of the matching use of the anti-backflow consumable D, the filterless pipette tip, and the pipette in Embodiment 4 of the present invention.

[0031] Figure 15 is Figure 14 an enlarged view of part D in

[0032] Figure 16 It is a schematic diagram of the state where the filterless pipette tip is removed from the pipette in Embodiment 4 of the present invention.

[0033] In the figure: 1 - pipette, 101 - hanging handle, 102 - piston rod, 103 - pipette tip removal button, 104 - negative pressure tube, 105 - pipette tip removal tube; 2 - pipette tip with filter, 201 - filter X; 3 - anti-backflow consumable, 301 - guiding part, 302 - disconnection and connection part, 303 - filter installation part, 304 - filter A; 4 - filterless pipette tip, 5 - pipette tip removal tube extension, 501 - connecting head, 502 - hitting head tube. Specific Embodiments

[0034] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0035] In the prior art, a hanging handle 101, a piston rod 102, a pipette tip removal button 103, a negative pressure tube 104, and a pipette tip removal tube 105 are provided on the pipette. By operating the piston rod 102 to provide negative pressure for the negative pressure tube 104, the pipette tip installed at the lower end of the negative pressure tube 104 sucks the sample; by operating the pipette tip removal button 103, the pipette tip removal button 103 drives the pipette tip removal tube 105 to knock off the pipette tip, separating the pipette from the pipette tip. In the prior art, when the pipette tip removal tube 105 is in the upper position, the lower end surface of the pipette tip removal tube 105 is above the lower end surface of the negative pressure tube 104 (at this time, the pipette tip can be installed); when the pipette tip removal tube 105 is in the lower position, the lower end surface of the pipette tip removal tube 105 is not higher than the lower end surface of the negative pressure tube 104 (at this time, the pipette tip is knocked off).

[0036] The above are all prior arts, and there are mature products on the market for sale. Therefore, the working principle of the pipette tip removal button, the working principle of the piston rod 102, and the related structures will not be described in detail below.

[0037] Example 1

[0038] As Figure 6 shown, a pipette anti-backflow consumable A includes a transparent hollow rotary consumable body, which successively includes a guiding part 301, a disassembly and connection part 302, and a filter element installation part 303 connected integrally from top to bottom. The guiding part 301 is a hollow frustum shape with a smaller upper part and a larger lower part. The disassembly and connection part 302 is used to be inserted into the negative pressure tube 104 of the pipette. The outer circumference of the disassembly and connection part 302 is provided with a soft rubber layer, and the soft rubber layer is in interference fit with the inner wall of the negative pressure tube 104; the outer diameter of the filter element installation part 303 is R, where r0 < R < R0, and r0 is the inner diameter of the negative pressure tube 104 of the pipette 1, and R0 is the outer diameter of the negative pressure tube 104 of the pipette 1. The height H of the filter element installation part 303 is ≤ 15 mm; a filter element A 304 is fixedly arranged in the cavity of the filter element installation part 303, and the material on the outer surface of the filter element A 304 is selected as an environmentally friendly water-sensitive color-changing material known in the prior art, which changes color when encountering water.

[0039] This example is applicable to existing pipettes and does not require any modification to the pipette during use. As Figure 5 and Figure 6 shown, first insert the pipette anti-backflow consumable A into the negative pressure tube 104 of the pipette, and then normally insert the filterless tip 4 on the outer circumference of the negative pressure tube 104 of the pipette. The use of the pipette anti-backflow consumable A does not affect the removal of the tip by the tip removal tube 105, as Figure 7 shown.

[0040] Example 2

[0041] As Figure 9 shown, a difference between a pipette anti-backflow consumable B and the pipette anti-backflow consumable A in Example 1 is that an external thread A is provided on the outer circumference of the disassembly and connection part 302, and it is detachably fixedly connected to the negative pressure tube 104 of the pipette through the external thread A; a soft rubber layer is not provided on the outer circumference of the disassembly and connection part 302, and the guiding part 301 is not provided, and the other features are exactly the same as those of the pipette anti-backflow consumable A in Example 1.

[0042] When using this example, a simple modification to the existing pipette is required. The lower end of the negative pressure tube 104 needs to be provided with an internal thread A, and the internal thread A is used for screwing with the external thread A on the pipette anti-backflow consumable B.

[0043] When in use, fix the pipette anti-backflow consumable B to the lower end of the negative pressure tube 104 through threads, and then normally insert the filterless tip 4 on the outer circumference of the negative pressure tube 104 of the pipette. The use of the pipette anti-backflow consumable A does not affect the removal of the tip by the tip removal tube 105, as Figure 10 shown.

[0044] Example 3

[0045] As shown Figure 12 in the figure, a pipette anti-backflow consumable C includes a transparent hollow rotary consumable body. The consumable body successively includes a detachable connection part 302 and a filter element installation part 303 connected integrally from top to bottom. The detachable connection part 302 is used for fixedly connecting with the negative pressure tube 104 of the pipette through threads. The outer diameter of the filter element installation part 303 is R, and R = R0, where R0 is the outer diameter of the negative pressure tube 104 of the pipette 1. The height H of the filter element installation part 303 is ≥50 mm. A filter element A304 is fixedly arranged at the top in the cavity of the filter element installation part 303. The filter element A304 is arranged at the top of the cavity of the filter element installation part 303, and the filter element A304 changes color when encountering water.

[0046] When using this embodiment, the existing pipette needs to be simply modified. The lower end of the negative pressure tube 104 needs to be provided with threads for screwing connection with the detachable connection part 302 on the pipette anti-backflow consumable C (it can be that the negative pressure tube 104 is provided with external threads and the detachable connection part 302 is provided with internal threads; or the negative pressure tube 104 is provided with internal threads and the detachable connection part 302 is provided with external threads).

[0047] When in use, the pipette anti-backflow consumable C is fixedly connected to the lower end of the negative pressure tube 104 through threads. Since the length of the pipette anti-backflow consumable C is relatively long, the pipette tip cannot be directly installed on the negative pressure tube 104 of the pipette. In order to install the pipette tip and still retain the function of knocking off the pipette tip of the pipette tip removal tube 105, a pipette tip removal tube extension 5 is designed, and the pipette tip 4 without a filter element is sleeved on the outer periphery of the lower end of the pipette anti-backflow consumable C.

[0048] The pipette tip removal tube extension 5 includes a connection head 501 and a striking tube 502 connected integrally. The connection head 501 is fixedly connected to the pipette tip removal tube 105. The inner diameter of the striking tube 502 is ry, and ry = rx, where rx is the inner diameter of the pipette tip removal tube 105; when the pipette tip removal tube 105 is in the upper position, the lower end face of the striking tube 502 is above the lower end face of the filter element installation part 303 of the pipette anti-backflow consumable (at this time, the pipette tip can be installed at the lower end of the striking tube 502); when the pipette tip removal tube 105 is in the lower position, the lower end face of the striking tube 502 is not higher than the lower end face of the filter element installation part 303 of the pipette anti-backflow consumable (at this time, the pipette tip removal tube 105 drives the striking tube 502 to knock off the pipette tip, as Figure 13 shown

[0049] The lengthening of the pipette anti-backflow consumable C further effectively prevents the backflow substance from entering the pipette and ensures the cleanliness of the pipette.

[0050] Embodiment 4

[0051] As Figure 15As shown, a pipette anti-backflow consumable D is different from the pipette anti-backflow consumable C in Embodiment 3 in that the filter element A304 is arranged at the bottom of the cavity of the filter element installation part 303, and the rest of the structure is the same as that of the pipette anti-backflow consumable C in Embodiment 3.

[0052] When using this embodiment, a new pipette needs to be designed. Since the length of the pipette anti-backflow consumable D is relatively long and the pipette tip cannot be directly installed on the negative pressure tube 104 of the pipette, the pipette tip removal tube 105 of the existing pipette is lengthened. When in use, the pipette anti-backflow consumable D is fixed to the lower end of the negative pressure tube 104 by threads, and the filterless pipette tip 4 is sleeved on the outer periphery of the lower end of the pipette anti-backflow consumable D. When the pipette tip removal tube 105 is in the upper position, the lower end surface of the pipette tip removal tube 105 is above the lower end surface of the filter element installation part 303 of the pipette anti-backflow consumable; when the pipette tip removal tube 105 is in the lower position, the lower end surface of the pipette tip removal tube 105 is not higher than the lower end surface of the filter element installation part 303 of the pipette anti-backflow consumable.

[0053] In the process of using the above 4 embodiments, if the filter element A304 in the pipette anti-backflow consumable does not change color, it means that no backflow phenomenon occurs during the pipetting operation, and only the filterless pipette tip 4 needs to be replaced; if it is found that the filter element A304 in the pipette anti-backflow consumable changes color, it means that backflow occurs during the pipetting operation, and a new pipette anti-backflow consumable needs to be replaced.

[0054] In the description of the present invention, the words indicating the orientation or positional relationship such as "inner", "outer", "upper", "lower", "front", "rear", etc. are only for the convenience of describing the present invention, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be construed as a limitation to the present invention.

[0055] The above are only several embodiments of the present invention. The protection scope of the present invention is not limited to the above embodiments. It should be noted that for those of ordinary skill in the art in this technical field, several improvements and refinements made without departing from the idea of the present invention fall within the protection scope of the present invention.

Claims

1. A pipette anti-backflow consumable, characterized by: The consumable body comprises a hollow rotary consumable body, which comprises, from top to bottom, a detachable portion (302) and a filter element mounting portion (303) which are integrally connected, the detachable portion (302) being used to be detachably fixedly connected to a negative pressure tube (104) of a liquid transfer gun, the outer diameter of the filter element mounting portion (303) being R, r0<R<R0, wherein r0 is the inner diameter of the negative pressure tube (104) of the liquid transfer gun (1), R0 is the outer diameter of the negative pressure tube (104) of the liquid transfer gun (1), and the height H of the filter element mounting portion (303) is ≤15 mm; a filter element A (304) is fixedly arranged in the cavity of the filter element mounting portion (303), and the material of the outer surface of the filter element A (304) is a material that changes color when in contact with water.

2. The pipette anti-backflow consumable according to claim 1, characterized in that: The detachable fixed connection between the detachable portion (302) of the consumable body and the negative pressure tube (104) of the pipette gun is achieved by plugging. The outer circumference of the detachable portion (302) is provided with a soft rubber layer, and the soft rubber layer and the inner wall of the negative pressure tube (104) are interference fit. A guide portion (301) is provided above the detachable portion (302), and the guide portion (301) is in the shape of a hollow truncated cone that is small at the top and wide at the bottom.

3. The pipette anti-backflow consumable according to claim 1, characterized in that: The detachable fixed connection between the detachable portion (302) of the consumable body and the negative pressure tube (104) of the pipette gun is a threaded connection, and an external thread A is provided on the outer circumference of the detachable portion (302).

4. A pipette gun, comprising a negative pressure tube (104) and a gun head tube (105) sleeved on the outside of the negative pressure tube (104); when the gun head tube (105) is in an upper position, the lower end surface of the gun head tube (105) is located above the lower end surface of the negative pressure tube (104); when the gun head tube (105) is in a lower position, the lower end surface of the gun head tube (105) is not higher than the lower end surface of the negative pressure tube (104); the lower end of the negative pressure tube (104) is used to connect to the gun head, and is characterized in that: Used in conjunction with the pipette anti-backflow consumables of claim 3, the lower end of the negative pressure tube (104) is provided with an internal thread A, and the internal thread A is used to be threadedly connected with the external thread A on the pipette anti-backflow consumables.

5. A pipette anti-backflow consumable, characterized by: The consumable body comprises a hollow rotary consumable body, which comprises, from top to bottom, a detachable portion (302) and a filter element mounting portion (303) which are integrally connected, the detachable portion (302) being used to be fixedly connected to a negative pressure tube (104) of a liquid transfer gun via a thread, the outer diameter of the filter element mounting portion (303) being R, R=R0, R0 being the outer diameter of the negative pressure tube (104) of the liquid transfer gun (1), the height H of the filter element mounting portion (303) being ≥50 mm, a filter element A (304) being fixedly provided at the top of the cavity of the filter element mounting portion (303), and the material of the outer surface of the filter element A (304) being a material that changes color when exposed to water.

6. A pipette gun, comprising a negative pressure tube (104) and a pipette head tube (105) sleeved on the outside of the negative pressure tube (104), wherein when the pipette head tube (105) is in an upper position, the lower end surface of the pipette head tube (105) is located above the lower end surface of the negative pressure tube (104); when the pipette head tube (105) is in a lower position, the lower end surface of the pipette head tube (105) is not higher than the lower end surface of the negative pressure tube (104); wherein: The negative pressure tube (104) is used for matching with the pipette anti-backflow consumables of claim 5, wherein the lower end of the negative pressure tube (104) is provided with a thread B, and the thread B is used to be screwed with the pipette anti-backflow consumables; the bottom end of the pipette anti-backflow consumables is used to be connected to the gun head; and the negative pressure tube (104) further comprises a hollow rotary gun head unloading tube extension piece (5), wherein the gun head unloading tube extension piece (5) comprises an integrally connected connecting head (501) and a head tube (502), wherein the connecting head (501) and the gun head unloading tube (105 ) are fixedly connected, the inner diameter of the header tube (502) is ry, ry=rx, rx is the inner diameter of the gun unloading head tube (105); when the gun unloading head tube (105) is in the upper position, the lower end surface of the header tube (502) is located above the lower end surface of the filter element mounting portion (303) of the anti-backflow consumables of the pipette; when the gun unloading head tube (105) is in the lower position, the lower end surface of the header tube (502) is not higher than the lower end surface of the filter element mounting portion (303) of the anti-backflow consumables of the pipette.

7. A pipette gun, comprising a negative pressure tube (104) and a pipette head tube (105) sleeved outside the negative pressure tube (104), characterized in that: Used in conjunction with the pipette anti-backflow consumables of claim 5, the lower end of the negative pressure tube (104) is provided with a thread C, and the thread C is used to be screwed with the pipette anti-backflow consumables; the bottom end of the pipette anti-backflow consumables is used to connect with the gun head; when the gun head removal tube (105) is in the upper position, the lower end surface of the gun head removal tube (105) is located above the lower end surface of the filter element mounting portion (303) of the pipette anti-backflow consumables; when the gun head removal tube (105) is in the lower position, the lower end surface of the gun head removal tube (105) is not higher than the lower end surface of the filter element mounting portion (303) of the pipette anti-backflow consumables.

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