Culture device for inducing differentiation of pancreatic duct stem cells

Abstract

The utility model discloses a culture device for inducing differentiation of pancreatic duct stem cells, and relates to the technical field of culture devices. The culture device for inducing pancreatic duct stem cell differentiation comprises a culture box main body, the culture box main body is internally provided with a culture device, the culture device comprises a culture solution adding unit and an observation unit, the culture solution adding unit comprises a placing disc, a culture dish is arranged on the placing disc, and a movable track is arranged on the placing disc; embedded seats are symmetrically arranged in the movable track, a communicating plate is arranged at the embedded groove in each embedded seat, and a U-shaped pipe is arranged on each communicating plate. According to the utility model, on the basis that the placing disc does not rotate, the embedding seat in the movable track does circular motion to finish adding, so that the placing disc is not subjected to direct driving force, the culture dishes on the placing disc are not subjected to centrifugal force and the like, and the culture solution in the culture dishes is promoted to be stable; and the limiting grooves formed in the placement disc further ensure stable placement of the culture dishes.

Description

Technical Field

[0001] The utility model relates to the technical field of culture devices, in particular to a culture device for inducing the differentiation of pancreatic duct stem cells. Background Technique

[0002] Pancreatic duct stem cells are stem cells existing in the pancreatic tissues of animals, which can self-renew and have multi-directional differentiation potential. Isolating and cloning pancreatic duct stem cells in vitro and inducing their differentiation into functional islets can be used as a high-quality cell source for in vitro β-cell regeneration, and at the same time provide a new material source for the treatment of diabetes by in vitro islet cell transplantation. Among them, a stem cell incubator is commonly used for pancreatic duct stem cells, which is a commonly used instrument in the stem cell culture project.

[0003] For example, a stem cell culture device disclosed in Chinese Patent Publication No. CN220183287U. This stem cell culture device is provided with through holes at the top of the box body, a rotating assembly and a pushing assembly are arranged inside the box body, a rotating disk is arranged on the rotating assembly, and placing holes for placing culture dishes are arranged on the rotating disk. The rotating assembly drives the rotating disk to rotate directly below the through hole, and the pushing assembly pushes the culture dish to the through hole, and the culture solution is added through the through hole. Therefore, it avoids the pollution of the stem cell tissue caused by bacteria in the air falling into the culture dish, thereby improving the effect of stem cell culture; in addition, a partition plate is arranged inside the box body to divide the box body into a culture chamber and a heating chamber, and a heating system is arranged in the heating chamber, and the required temperature for stem cell culture can be created through the heating system.

[0004] However, the above device adds the culture solution to the culture dish mainly by dripping through the through hole. However, there are multiple groups of culture dishes arranged inside the box body. Therefore, when adding to different culture dishes, the device uses the design of bevel gear meshing to make the rotating disk on which the culture dish is placed rotate, and then the fixed-position through hole completes the dripping of the culture solution into different culture dishes. However, this transmission method is very likely to cause large vibrations of the rotating disk, and factors such as centrifugal force generated during rotation are very likely to cause the culture solution in the culture dish to overflow or spill, etc., thus affecting the normal culture of stem cells. Content of the Utility Model

[0005] Aiming at the deficiencies of the prior art, the utility model provides a culture device for inducing the differentiation of pancreatic duct stem cells, and solves the problems put forward in the above background technique.

[0006] To achieve the above purposes, the utility model is realized through the following technical solutions: A culture device for inducing the differentiation of pancreatic duct stem cells, including a main body of the culture box, a culture device for the differentiation of pancreatic duct stem cells is arranged inside the main body of the culture box, the culture device includes a culture solution adding unit and an observation unit, and both the culture solution adding unit and the observation unit are installed at the culture chamber inside the main body of the culture box;

[0007] The culture medium adding unit includes a placement tray, on which there are culture dishes for culturing cells. There are multiple groups of the culture dishes, which are arranged in an array with the center of the placement tray as the base point. An active track is provided on the placement tray, and fitting seats are symmetrically arranged in the active track. At the fitting groove inside each fitting seat, there is a connecting plate, and on each connecting plate, there is a U-shaped tube for adding nutrient solution. The other end of each U-shaped tube is connected to a storage tank installed on the top of the corresponding connecting plate.

[0008] A further improvement of the technical solution of the present utility model is that: the placement tray is inserted into each group of culture dishes through the limit grooves provided thereon. The placement tray is movably connected to each fitting seat through the active track. Each fitting seat is limit-inserted into the connecting plate through the fitting groove. The connecting plate is connected to the U-shaped tube, and the U-shaped tube is connected to the storage tank through an electric control valve.

[0009] It should be noted that, in this embodiment, the other end of the U-shaped tube can also be connected to a dropper arranged outside. At this time, the material of the U-shaped tube needs to be replaced with a rubber hose and the length should be sufficient, because the U-shaped tube made of rubber will move along with the movement during the circumferential movement of the fitting seat in the active track. Therefore, the length needs to be sufficient, and a corresponding seal needs to be provided at the place where the U-shaped tube penetrates the box body. The technical advantage of this is that there is no need for an electric control valve, and the amount of nutrient solution dropped can be directly controlled manually. However, no matter which method is used, it is difficult to cause the culture dishes to shake, and the nutrient solution inside is not easy to overflow or shake.

[0010] A further improvement of the technical solution of the present utility model is that: an arc-shaped plate for connection is arranged between the two fitting seats. A number of groups of vertical rods are arranged on the arc-shaped plate. The other end of each group of vertical rods penetrates the placement tray through the active track and is connected to a linkage disk. A drive shaft connected to a drive motor is arranged at the center of the linkage disk.

[0011] In this embodiment, the drive motor drives the drive shaft to rotate. While the linkage disk makes a circumferential movement, the arc-shaped plate at the other end is driven to make a circumferential movement due to the vertical rods provided. And because one end of the arc-shaped plate connects the two fitting seats, the fitting seats rotate circumferentially in the active track of the placement tray, so as to add nutrient solution to the culture dishes at different positions of the placement tray.

[0012] It should be noted that, different from the prior art, the technical solution provided in this embodiment is based on the fact that the placement tray does not rotate, and the circumferential movement of the fitting seats in the active track is completed for addition. Therefore, the placement tray will not receive a direct driving force, and the culture dishes thereon will not be affected by centrifugal force and other effects, which promotes the stability of the culture solution in the culture dishes. Moreover, the limit grooves provided on the placement tray further ensure the stable placement of the culture dishes.

[0013] A further improvement of the technical solution of the present utility model lies in that both ends of the arc-shaped plate are welded to the corresponding fitting seats, each group of vertical rods is detachably connected to the arc-shaped plate through fasteners, the other end of each group of vertical rods is fixedly connected to a linkage disk, the linkage disk is in transmission connection with a driving shaft, and the driving shaft is connected to a driving motor through a coupling.

[0014] A further improvement of the technical solution of the present utility model lies in that the observation unit includes an observation port installed on the top of the incubator main body, a microscope assembly for observation is arranged on the observation port, and lifting seats are symmetrically arranged on both sides inside the incubator main body. A bearing plate is arranged in each lifting groove opened in each lifting seat, a base installed at the bottom of the placement tray is arranged at the other end of each bearing plate, and a number of groups of electric push rods are arranged on each bearing plate. Before or after adding the culture solution, the observation unit can use the electric push rods to lift the base and the placement tray assembly as a whole, and cooperate with the microscope assembly for observation, so as to effectively and real-time control the culture environment of induced pancreatic duct stem cells, improve the convenience of culture and complete the monitoring of the culture environment.

[0015] A further improvement of the technical solution of the present utility model lies in that the microscope assembly is installed on the incubator main body through the observation port in a limited way, each lifting seat is fixedly installed inside the incubator main body, each lifting seat is slidably connected with the corresponding bearing plate through a lifting groove, each bearing plate is fixedly connected with the base, the other end of each electric push rod is connected with the corresponding bearing plate, and the base is provided with a through groove at the same longitudinal position as the movable track. Beneficial effects

[0016] The present utility model provides a culture device for inducing the differentiation of pancreatic duct stem cells. Compared with the prior art, it has the following beneficial effects:

[0017] 1. For the culture device for inducing the differentiation of pancreatic duct stem cells, based on the fact that the placement tray in the culture solution adding unit does not rotate, and the fitting seats in the movable track complete the addition through circular motion, the placement tray will not be directly driven, so the culture dish on it will not be affected by centrifugal force and other forces, which promotes the stability of the culture solution in the culture dish, and the limiting grooves arranged on the placement tray further ensure the stable placement of the culture dish.

[0018] 2. For the culture device for inducing the differentiation of pancreatic duct stem cells, before or after adding the culture solution, the observation unit can use the electric push rods to lift the base and the placement tray assembly as a whole, and cooperate with the microscope assembly for observation, so as to effectively and real-time control the culture environment of induced pancreatic duct stem cells, improve the convenience of culture and complete the monitoring of the culture environment. Description of the drawings

[0019] Figure 1This is a schematic structural diagram of the incubator main body of the present utility model;

[0020] Figure 2 This is a schematic diagram of the culture device of the present utility model;

[0021] Figure 3 This is a schematic diagram of the culture medium adding unit of the present utility model;

[0022] Figure 4 This is a schematic structural diagram of the culture medium adding unit of the present utility model.

[0023] In the figure: 1. Incubator main body; 201. Placing tray; 202. Petri dish; 203. Movable track; 204. Fitting seat; 205. Connecting plate; 206. U-shaped tube; 207. Storage tank; 208. Arc plate; 209. Vertical rod; 210. Linkage plate; 211. Driving shaft; 212. Driving motor; 301. Observation port; 302. Microscope assembly; 303. Lifting seat; 304. Bearing plate; 305. Base; 306. Electric push rod. Detailed implementation manners

[0024] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0025] The present utility model provides 3 technical solutions:

[0026] Figures 1-3 The first implementation manner is shown: A culture device for inducing the differentiation of pancreatic duct stem cells, including an incubator main body 1. A culture device for the differentiation of pancreatic duct stem cells is arranged in the incubator main body 1. The culture device includes a culture medium adding unit and an observation unit. Both the culture medium adding unit and the observation unit are installed at the culture chamber inside the incubator main body 1;

[0027] The culture medium adding unit includes a placing tray 201. Petri dishes 202 for culturing cells are arranged on the placing tray 201. There are multiple groups of Petri dishes 202 and they are arranged in an array with the center of the placing tray 201 as the base point. A movable track 203 is arranged on the placing tray 201. Fitting seats 204 are symmetrically arranged in the movable track 203. A connecting plate 205 is arranged at the fitting groove inside each fitting seat 204. U-shaped tubes 206 for adding nutrient solution are arranged on each connecting plate 205. The other end of each U-shaped tube 206 is connected and installed to a storage tank 207 at the top of the corresponding connecting plate 205.

[0028] The placement tray 201 is inserted into each group of culture dishes 202 through the limit slots provided thereon. The placement tray 201 is movably connected to each fitting seat 204 through the movable track 203. Each fitting seat 204 is limit-inserted into the connecting plate 205 through the fitting groove. The connecting plate 205 is connected to the U-shaped tube 206, and the U-shaped tube 206 is connected to the storage tank 207 through an electric control valve.

[0029] It should be noted that, in this embodiment, the other end of the U-shaped tube 206 can also be connected to a dropper provided outside. At this time, the material of the U-shaped tube 206 needs to be replaced with a rubber hose and the length is sufficient. Because the U-shaped tube 206 made of rubber will move along with the movement during the circumferential movement of the fitting seat 204 on the movable track 203, a sufficient length is required. And under this technical solution, a corresponding seal needs to be provided at the place where the U-shaped tube 206 penetrates the box body. The technical advantage of doing this is that there is no need for an electric control valve, and the amount of nutrient solution dripping can be directly controlled manually. However, no matter which method is used, it is difficult to cause the culture dish 202 to shake, and the nutrient solution inside is not easy to overflow or shake.

[0030] Figures 2-4 The second implementation manner is shown. The main difference from the first implementation manner is that: arc-shaped plates 208 for connection are provided between the two fitting seats 204. A number of groups of vertical rods 209 are provided on each arc-shaped plate 208. The other end of each group of vertical rods 209 penetrates through the placement tray 201 through the movable track 203 and is connected to a linkage disk 210. A drive shaft 211 connecting the drive motor 212 is provided at the center of the circle of the linkage disk 210.

[0031] In this embodiment, the drive motor 212 drives the drive shaft 211 to rotate. While the linkage disk 210 makes a circumferential movement, the arc-shaped plate 208 at the other end is driven to make a circumferential movement by the provided vertical rods 209. And because one end of the arc-shaped plate 208 connects the two fitting seats 204, the fitting seats 204 rotate circumferentially within the movable track 203 of the placement tray 201. Therefore, nutrient solution is added to the culture dishes 202 at different positions of the placement tray 201.

[0032] It should be noted that, different from the prior art, the technical solution provided in this embodiment is based on the fact that the placement tray 201 does not rotate, and the circumferential movement of the fitting seats 204 in the movable track 203 is completed for addition. Therefore, the placement tray 201 will not be directly driven, and the culture dishes 202 thereon will not be affected by centrifugal force and other effects, which promotes the stability of the culture solution in the culture dishes 202. And the limit slots provided on the placement tray 201 further ensure the stable placement of the culture dishes 202.

[0033] Both ends of the arc-shaped plate 208 are welded to the corresponding fitting seats 204. Each group of vertical rods 209 is detachably connected to the arc-shaped plate 208 through fasteners. The other end of each group of vertical rods 209 is fixedly connected to the linkage disk 210. The linkage disk 210 is drivingly connected to the drive shaft 211. The drive shaft 211 is connected to the drive motor 212 through a coupling.

[0034] Figures 1-2 Figures 4 show the third embodiment. The main difference from the first embodiment is that the observation unit includes an observation port 301 installed on the top of the incubator main body 1. A microscope assembly 302 for observation is provided on the observation port 301. Lifting seats 303 are symmetrically arranged on both sides inside the incubator main body 1. A bearing plate 304 is arranged in each lifting groove opened in each lifting seat 303. The other end of each bearing plate 304 is provided with a base 305 installed at the bottom of the placement tray 201. A number of groups of electric push rods 306 are arranged on each bearing plate 304. Before or after adding the culture solution, the observation unit can use the electric push rods 306 to lift the base 305 and the placement tray 201 assembly as a whole, and cooperate with the microscope assembly 302 for observation, so as to effectively and real-time control the culture environment of induced pancreatic duct stem cells, improve the convenience of culture and complete the monitoring of the culture environment.

[0035] The microscope assembly 302 is limited and installed on the incubator main body 1 through the observation port 301. Each lifting seat 303 is fixedly installed inside the incubator main body 1. Each lifting seat 303 is slidably connected to the corresponding bearing plate 304 through the lifting groove. Each bearing plate 304 is fixedly connected to the base 305. The other end of each electric push rod 306 is connected to the corresponding bearing plate 304. The base 305 is provided with a through groove at the same longitudinal position as the movable track 203.

[0036] At the same time, the content not described in detail in this specification belongs to the prior art well-known to those skilled in the art, and the model parameters of each electrical appliance are not specifically limited, and conventional equipment can be used.

[0037] During use, randomly thaw the 70th-generation rat pancreatic duct stem cells. After resuscitation, at 1×10 5Inoculate in a 24-well plate and place it in the incubator main body 1 at 37°C with 5% CO2 and saturated humidity. Place the culture dish 202 stably in the placement tray 201 through the limiting groove. At this time, close the incubator main body 1. After 12 hours, observe the cell growth state through the microscope assembly 302. When additional culture medium is needed, rotate the drive shaft 211 according to the drive of the drive motor 212. While the linkage disk 210 makes a circular motion, the vertical rod 209 drives the arc-shaped plate 208 at the other end to make a circular motion. Since one end of the arc-shaped plate 208 is connected to two fitting seats 204, the fitting seats 204 rotate circularly in the moving track 203 of the placement tray 201. Therefore, nutrient solution is added to the culture dishes 202 at different positions of the placement tray 201 to replace the fresh culture medium and continue the amplification culture for 2 days.

[0038] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or elements inherent to such process, method, article or device.

[0039] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A culture device for inducing pancreatic ductal stem cell differentiation, comprising a culture box body (1), characterized in that: The incubator body (1) is provided with a culture device for pancreatic ductal stem cell differentiation, the culture device comprising a culture fluid addition unit and an observation unit, the culture fluid addition unit and the observation unit are both installed in the culture cavity inside the incubator body (1); The culture fluid adding unit comprises a placement plate (201), on which a culture dish (202) for culturing cells is arranged, wherein a plurality of groups of the culture dishes (202) are arranged in an array with the center of the placement plate (201) as a base point, and a movable track (203) is arranged on the placement plate (201), wherein a fitting seat (204) is symmetrically arranged in the movable track (203), and a connecting plate (205) is arranged at a fitting groove built into each of the fitting seats (204), and each of the connecting plates (205) is provided with a U-shaped tube (206) for adding nutrient solution, and the other end of each of the U-shaped tubes (206) is connected to a storage box (207) installed on the top of the corresponding connecting plate (205).

2. A culture device for inducing pancreatic ductal stem cell differentiation according to claim 1, characterized in that: The placement plate (201) is plugged into each group of culture dishes (202) via a limiting groove provided thereon, the placement plate (201) is movably connected to each engaging seat (204) via a movable track (203), each engaging seat (204) is limitedly plugged into a connecting plate (205) via an engaging groove, the connecting plate (205) is connected to a U-shaped tube (206), and the U-shaped tube (206) is connected to a storage box (207) via an electric control valve.

3. A culture device for inducing pancreatic ductal stem cell differentiation according to claim 1, characterized in that: An arc-shaped plate (208) for connection is provided between the two engaging seats (204), and a plurality of groups of vertical rods (209) are provided on the arc-shaped plate (208). The other end of each group of vertical rods (209) passes through the placement plate (201) through the movable track (203) and is connected to a linkage plate (210). A driving shaft (211) connected to a driving motor (212) is provided at the center of the linkage plate (210).

4. A culture device for inducing pancreatic ductal stem cell differentiation according to claim 3, characterized in that: Both ends of the arc-shaped plate (208) are welded to the corresponding engaging seats (204); each set of vertical rods (209) is detachably connected to the arc-shaped plate (208) via fasteners; the other end of each set of vertical rods (209) is fixedly connected to a linkage disk (210); the linkage disk (210) is transmission-connected to a drive shaft (211); and the drive shaft (211) is connected to a drive motor (212) via a coupling.

5. The culture device for inducing pancreatic ductal stem cell differentiation according to claim 1, characterized in that: The observation unit comprises an observation port (301) installed on the top of the incubator body (1), a microscope assembly (302) for observation is arranged on the observation port (301), and lifting seats (303) are symmetrically arranged on both sides of the incubator body (1), and each lifting slot opened in the lifting seat (303) is provided with a bearing plate (304), and each bearing plate (304) is provided with a base (305) installed on the bottom of the placement plate (201) at the other end, and each bearing plate (304) is provided with a plurality of groups of electric push rods (306).

6. The culture device for inducing pancreatic ductal stem cell differentiation according to claim 5, characterized in that: The microscope assembly (302) is limitedly mounted on the incubator body (1) through the observation port (301), each lifting seat (303) is fixedly mounted inside the incubator body (1), each lifting seat (303) is slidably connected to a corresponding bearing plate (304) through a lifting slot, each bearing plate (304) is fixedly connected to a base (305), the other end of each electric push rod (306) is connected to a corresponding bearing plate (304), and the base (305) is provided with a through slot at the same longitudinal position as the movable track (203).

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