Laminar flow inspection tool for flow cytometer

Abstract

The utility model discloses a laminar flow inspection tool for a flow cytometer, and particularly relates to a flow cytometer, which comprises a transparent sheath flow device, the light supplementing plate is arranged at a preset distance from one side of the transparent sheath flow device; an acquisition end is provided with a high-speed camera of an objective lens, and the axis of the objective lens intersects with the center line of the sheath flow device and the light supplementing plate; wherein a sheath liquid tube channel of the sheath flow device is fixedly communicated with a peristaltic pump, and the peristaltic pump is fixedly communicated with a pure water bottle; the sheath flow device sample tube channel is fixedly communicated with a plunger pump, and an inlet of the plunger pump is fixedly communicated with a red ink bottle. According to the utility model, a result is directly detected instead of an intermediate link for detecting the assembly parts, so that the time for detecting intermediate parts in the production process of an integrated enterprise is shortened, and rapid screening of good products is facilitated.

Description

Technical Field

[0001] The utility model relates to a flow cytometer, in particular to a laminar flow inspection tooling for a flow cytometer. Background Technique

[0002] Flow cytometry (FcM) is a technology that can quickly and accurately perform multi-parameter quantitative analysis and sorting of the physical and chemical properties of single cells (or biological particles) by using a flow cytometer as a detection means.

[0003] The technology developed based on the laminar flow principle can realize the coaxial flow of two liquids. The sample cell flow is located at the axis and flows stably, and is wrapped with sheath fluid on the outside. After the stable liquid flow flows from the part with a larger cross-sectional area into the part with a smaller cross-sectional area, there is a focusing and shrinking effect, and the diameter of the cell flow is restricted to 10-20um, avoiding multiple cells from overlapping and entering the detection area.

[0004] However, in the prior art, for the laminar motion of viscous fluids, the trajectories of fluid micro-elements do not have obvious irregular pulsations, and there is only momentum exchange caused by molecular thermal motion between adjacent fluid layers. Therefore, by capturing the operating state of this momentum exchange, the laminar motion state of viscous fluids can be clearly obtained. Content of the Utility Model

[0005] The purpose of the utility model is to provide a laminar flow inspection tooling for a flow cytometer to solve the above problems.

[0006] To achieve the above purpose, the utility model provides the following technical solution: A laminar flow inspection tooling for a flow cytometer, comprising:

[0007] A transparent sheath flow cell;

[0008] A supplementary light plate arranged at a predetermined distance from one side of the transparent sheath flow cell;

[0009] A high-speed camera with an objective lens assembled at the acquisition end, and the axis of the objective lens intersects the center lines of the sheath flow cell and the supplementary light plate;

[0010] Wherein, a peristaltic pump is fixedly connected to the sheath fluid pipe channel of the sheath flow cell, and a pure water bottle is fixedly connected to the peristaltic pump;

[0011] A plunger pump is fixedly connected to the sample tube channel of the sheath flow cell, and a red ink bottle is fixedly connected to the inlet of the plunger pump.

[0012] Preferably, the magnification of the mentioned objective lens is 100 times.

[0013] Preferably, the supplementary light plate is a rectangular plastic plate with a mirror film.

[0014] Preferably, a waste liquid pump is fixedly connected to the waste liquid pipe channel of the sheath flow cell.

[0015] In the above technical solution, a laminar flow inspection tool for a flow cytometer provided by the present utility model has the following beneficial effects: directly detecting the result, rather than detecting the intermediate links of the assembled parts, reducing the time for integrated enterprises to detect the intermediate parts during the production process, and contributing to the rapid screening of good products. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required in the embodiments. Obviously, the drawings in the following description are only some embodiments recorded in the present utility model, and those of ordinary skill in the art can also obtain other drawings based on these drawings.

[0017] Figure 1 It is a schematic structural diagram provided by an embodiment of the present utility model.

[0018] Description of the reference numerals:

[0019] 11. Waste liquid pump; 13. Plunger pump; 15. Peristaltic pump; 111. Supplementary light plate; 113. Sheath flow cell; 115. Objective lens; 117. High-speed camera. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0020] In order to enable those skilled in the art to better understand the technical solutions of the present utility model, the following will further introduce the present utility model in detail with reference to the drawings.

[0021] As Figure 1 shown, the present invention relates to a laminar flow inspection tool applicable to a flow cytometer, which is characterized by including:

[0022] A transparent sheath flow cell 113;

[0023] A supplementary light plate 111 arranged at a predetermined distance from one side of the sheath flow cell 113;

[0024] A high-speed camera 117, the acquisition end of which is equipped with an objective lens 115, and the axis of the objective lens 115 intersects with the center lines of the sheath flow cell 113 and the supplementary light plate 111;

[0025] The sheath liquid pipe channel of the sheath flow cell 113 is fixedly connected to a peristaltic pump 15, and the other end of the peristaltic pump 15 is fixedly connected to a pure water bottle 33;

[0026] The sample pipe channel of the sheath flow cell 113 is fixedly connected to a plunger pump 13, and the inlet of the plunger pump 13 is fixedly connected to a red ink bottle 31.

[0027] Further, the magnification of the objective lens 115 is 100 times. Further still, the waste liquid pipe channel of the sheath flow cell 113 is fixedly connected to a waste liquid pump 11, and the outlet of the waste liquid pump 11 is directly connected to a waste liquid barrel.

[0028] Based on the above technical features, the usage method of this detection tooling includes:

[0029] S1. Fix the sheath flow cell 113 to the detection platform and suspend it.

[0030] S2. Connect the sample tube channel of the sheath flow cell 113 to the plunger pump 13, and connect the inlet of the plunger pump 13 to the red ink bottle 31.

[0031] S3. Connect the sheath liquid tube channel of the sheath flow cell 113 to the peristaltic pump 15, and connect the inlet of the peristaltic pump 15 to the pure water bottle 33.

[0032] S4. Connect the waste liquid tube channel of the sheath flow cell 113 to the waste liquid pump 11, and the outlet of the waste liquid pump 11 is directly connected to the waste liquid barrel.

[0033] S5. After completing step S4, install the X100 objective lens 115 on the plane of the sheath flow cell 113, and ensure that the tube side of the X100 objective lens 115 corresponds to the detection surface of the sheath flow cell 113.

[0034] S6. After completing step S5, install a high-speed camera 117 behind the objective lens 115 and focus it so that the detection surface of the sheath flow cell 113 is clearly visible.

[0035] S7. Turn on the peristaltic pump 15 and inject pure water sheath liquid into the sheath flow cell 113.

[0036] S8. Turn on the waste liquid pump 11 to increase the negative pressure at the outlet of the sheath flow cell 113.

[0037] S9. The plunger pump extracts red ink 31 and injects it into the sheath flow cell 113.

[0038] S10. Connect to the high-speed camera 117 through a computer to observe the results and calculate the laminar flow data.

[0039] It should be noted that by connecting to the high-speed camera 117 through a computer to observe the laminar flow state, after step S6 is completed, the detection surface of the sheath flow cell is clearly visible. In step S10, the displayed results are as follows: the middle detection surface of the sheath flow cell 113 presents clear pure water and red ink channels. Through computer measurement results, the laminar flow state position of the sample is a 15um * 40um cross-section.

[0040] Only some exemplary embodiments of the present utility model have been described by way of illustration. Without doubt, for those of ordinary skill in the art, the described embodiments can be modified in various different ways without departing from the spirit and scope of the present utility model. Therefore, the above drawings and description are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present utility model.

Claims

1. A laminar flow inspection tool for a flow cytometer, characterized in that: include: a transparent sheath flow device (113); a fill light plate (111) arranged at a predetermined distance from one side of the sheath flow device (113); A high-speed camera (117) equipped with an objective lens (115) is mounted at the collection end, wherein the axis of the objective lens (115) intersects with the center lines of the sheath flow device (113) and the fill light plate (111); Wherein, the sheath liquid tube channel of the sheath flow device (113) is fixedly connected to a peristaltic pump (15), and the peristaltic pump (15) is fixedly connected to a pure water bottle (33); The sample tube channel of the sheath flow device (113) is fixedly connected to a plunger pump (13), and the inlet of the plunger pump (13) is fixedly connected to a red ink bottle (31).

2. The laminar flow inspection tool for flow cytometer according to claim 1, characterized in that: The magnification of the objective lens (115) is 100 times.

3. The laminar flow inspection tool for flow cytometer according to claim 1, characterized in that: The light-filling plate (111) is a rectangular plastic plate with a mirror film.

4. The laminar flow inspection tool for flow cytometer according to claim 1, characterized in that: The waste liquid pipe channel of the sheath flow device (113) is fixedly connected to a waste liquid pump (11).

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