Perfusion orifice plate for co-culture
By designing multiple culture chambers and communication channels on the perfusion orifice plate, the liquid circulation environment in vivo is simulated, and the problem that the existing technology is difficult to effectively simulate the internal environment is solved, and more efficient cell co-culture and new drug development are achieved.
Patent Information
- Application Number
- CN202421806624.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-29
- Publication Date
- 2025-06-27
- Estimated Expiration
- 2034-07-29
AI Technical Summary
The existing co-culture technology is difficult to effectively simulate the fluid circulation environment in vivo, limiting the research on cell-to-cell interactions and the development of new drugs.
A perfusion orifice plate for co-culture was designed, and by setting multiple culture chambers and communication channels on the perfusion orifice plate, the liquid circulation environment in vivo was simulated, and a liquid reservoir was set between each culture chamber to improve the edge effect.
Cell co-culture is achieved closer to the internal environment, which enhances the ability to study interactions between cells, improves the efficiency of new drug development and screening, and reduces research costs.
Smart Images
Figure CN223033392U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of medical equipment, in particular to a perfusion orifice plate for co-culture. Background Art
[0002] The significance of cell co-culture is to reproduce a growth system closer to the in-vivo environment, enabling cells to communicate with each other, influence growth and proliferation. Since the late 1980s, co-culture has become an important approach in modern cell research; it co-cultures two or more types of cells in almost the same environment to study the interaction between cells. Co-culture can better restore the behavior and response of cells in vivo, so it is widely adopted; generally speaking, the main functions of cell co-culture are manifested in inducing cell directed differentiation, regulating amplification, maintaining cell function and activity, increasing metabolite production, and conducting drug efficacy tests, etc.
[0003] Co-culture provides a convenient co-culture solution. Dozens of co-culture chambers are constructed through a special multi-well culture plate, which can simulate the in-vivo liquid circulation environment to study the interaction between cells, including autocrine and paracrine interactions; this not only helps researchers understand the complex interactions between cells, but also provides an effective way for the development and screening of new drugs; in addition, the stable culture environment, large-scale culture capacity, and standardized operation of co-culture greatly improve the efficiency of co-culture, shorten the research cycle, and effectively help users save costs. Content of the Utility Model
[0004] The main purpose of the utility model is to provide a perfusion orifice plate for co-culture.
[0005] To achieve the above purpose, the technical solution adopted by the utility model is as follows:
[0006] A perfusion orifice plate for co-culture, including a perfusion orifice plate body. A culture groove is fixedly opened on the upper end surface of the perfusion orifice plate body. A plurality of sub-groove isolation rings are fixedly arranged in the culture groove. The sub-groove isolation rings divide the culture groove of the perfusion orifice plate body into multiple culture chambers. Communication channels are fixedly opened on the sub-groove isolation rings between every two culture chambers.
[0007] Further, a liquid storage groove is formed between the culture groove and the outer wall of the sub-groove isolation ring, and there are multiple such liquid storage grooves.
[0008] Further, an installation groove is also opened on the perfusion orifice plate body, and a clamping joint is fixedly installed in the installation groove.
[0009] Further, the sub-groove isolation ring and the perfusion orifice plate body are integrally formed and made of the same material.
[0010] Further, a clamping groove is formed on the outer ring of the upper end surface of the perfusion orifice plate body, and an upper cover plate is slidably clamped in the clamping groove.
[0011] Further, the height of the sub-groove isolation ring is not higher than the height of the culture tank.
[0012] Compared with the prior art, the beneficial effects of the present utility model are as follows:
[0013] The perfusion orifice plate of the present utility model is a special multi-well culture plate designed specifically for co-culture and perfusion culture. Communication channels with a certain angle are designed between the culture chambers to achieve co-culture activities between different cells. In addition, liquid storage tanks are designed between the culture chambers. During use, culture medium or sterile water needs to be filled in to improve the edge effect. BRIEF DESCRIPTION OF THE DRAWINGS
[0014] Figure 1 is a perspective view of the present utility model;
[0015] Figure 2 is a top view of the present utility model.
[0016] REFERENCE SIGNS
[0017] 1. Perfusion orifice plate body; 2. Sub-groove isolation ring; 3. Culture chamber; 4. Communication channel; 5. Liquid storage tank; 6. Installation groove; 7. Clamping head; 8. Clamping groove. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0018] The following elaborates on the preferred embodiments of the present utility model with reference to the accompanying drawings, so that the advantages and features of the present utility model can be more easily understood by those skilled in the art, thereby making the protection scope of the present utility model more clearly defined.
[0019] See Figure 1-2 As shown, a perfusion orifice plate for co-culture includes a perfusion orifice plate body. A culture tank is fixedly formed on the upper end surface of the perfusion orifice plate body. A plurality of sub-groove isolation rings are fixedly arranged in the culture tank. The sub-groove isolation rings divide the culture tank of the perfusion orifice plate body into a plurality of culture chambers. Communication channels are fixedly formed on the sub-groove isolation rings between every two culture chambers.
[0020] A liquid storage tank is formed between the outer wall of the culture tank and the sub-groove isolation ring, and the liquid storage tank includes a plurality of them.
[0021] An installation groove is further formed on the perfusion orifice plate body, and a clamping head is fixedly installed in the installation groove.
[0022] The sub-groove isolation ring and the perfusion orifice plate body are integrally formed and made of the same material.
[0023] A clamping groove is formed on the outer ring of the upper end surface of the perfusion orifice plate body, and an upper cover plate is slidably clamped in the clamping groove.
[0024] The height of the sub-groove isolation ring is not higher than the height of the culture tank.
[0025] The above is only the preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiment. Any equivalent modification or change made by those of ordinary skill in the art according to the disclosure of the present invention shall be included in the protection scope recorded in the claims.
Claims
1. A perfusion orifice plate for co-culture, comprising a perfusion orifice plate body, characterized in that: A culture groove is fixedly provided on the upper end surface of the perfusion orifice plate body, and a plurality of dividing groove isolating rings are fixedly provided in the culture groove. The dividing groove isolating rings divide the culture groove of the perfusion orifice plate body into a plurality of culture chambers, and a connecting channel is fixedly provided on the dividing groove isolating rings between every two culture chambers.
2. A perfusion plate for co-culture according to claim 1, characterized in that: A liquid storage tank is formed between the culture tank and the outer wall of the sub-tank isolation ring, and the liquid storage tank includes multiple liquid storage tanks.
3. A perfusion plate for co-culture according to claim 1, characterized in that: The perfusion orifice plate body is also provided with an installation groove, in which a card joint is fixedly installed.
4. A perfusion plate for co-culture according to claim 1, characterized in that: The slot isolation ring is integrally formed with the perfusion orifice plate body and is made of the same material.
5. The perfusion plate for co-culture according to claim 1, characterized in that: A clamping groove is provided on the outer ring of the upper end surface of the perfusion orifice plate body, and the upper cover plate is slidably clamped in the clamping groove.
6. A perfusion plate for co-culture according to claim 1, characterized in that: The height of the sub-tank isolation ring is not higher than the height of the culture tank.