Ultrasonic treatment device for experimental sample

Abstract

The utility model provides an ultrasonic treatment device for an experimental sample, and relates to the technical field of ultrasonic treatment. The ultrasonic treatment device for the experimental sample comprises a loading cover box which is provided with a plurality of panel holes used for loading sample centrifugal tubes and a plurality of refrigeration strips used for maintaining low temperature, the edge of the loading cover box is provided with a plurality of concave holes, and the panel holes and the refrigeration strips are arranged adjacently; the supporting bottom plate is used for supporting the loading cover box, a plurality of supporting columns are arranged on the edge of the supporting bottom plate, and the supporting columns and the concave holes are oppositely arranged and can be correspondingly connected with one another; and the ultrasonic generation part is used for placing the supporting bottom plate and the loading cover box and carrying out ultrasonic treatment on the test sample in the sample centrifugal tube. According to the integrated design, frequent sample transfer among different devices is avoided, and the operation efficiency is improved. A plurality of refrigeration strips can also provide a low-temperature environment for the sample in the sample centrifugal tube, so that manual operation can be reduced on the whole, and the ultrasonic treatment efficiency is improved.

Description

Technical Field

[0001] The present application relates to the technical field of ultrasonic processing, for example, to an ultrasonic processing device, a control method, an electronic device, and a storage medium for experimental samples. Background Art

[0002] The pretreatment of experimental samples is a crucial link in the experimental process, and the quality of sample processing directly affects the accuracy and reliability of experimental results. As an efficient sample processing method, ultrasonic processing technology has been widely used in laboratories.

[0003] In the related art, when performing the pretreatment of experimental samples, a large number of manual operations are generally required. Experimental personnel need to manually switch samples between different types of well plates frequently, such as the switching of traditional 96-well plate racks. The traditional 96-well plate rack is relatively heavy, and its material is not suitable for directly placing it into the ultrasonic generating part. Therefore, it is necessary to transfer and insert samples into the holes of a porous floating plate with a lighter weight one by one, then move the porous floating plate with a large number of test samples into the ultrasonic generating part, and then perform ultrasonic processing on the experimental samples. The whole process involves a large number of manual operations, which is greatly affected by human factors. Moreover, many samples require a low-temperature environment, and currently, it is necessary to manually change ice frequently, resulting in low ultrasonic processing efficiency. Utility Model Content

[0004] The present application aims to provide an ultrasonic processing device for experimental samples, which can perform batch transfer and ultrasonic treatment of samples, and provide the required low-temperature environment for the samples, thereby improving the processing efficiency of sample ultrasonic treatment.

[0005] According to one aspect of the present application, there is provided an ultrasonic processing device for experimental samples, including: a loading cover box, provided with a plurality of panel holes for loading sample centrifuge tubes and a plurality of refrigeration strips for maintaining low temperature, and a plurality of concave holes are arranged at the edge of the loading cover box, and the panel holes and the refrigeration strips are arranged adjacent to each other; a support bottom plate for supporting the loading cover box, and a plurality of support columns are arranged at the edge of the support bottom plate, and the plurality of support columns are respectively arranged opposite to the plurality of concave holes and can be correspondingly connected to each other; an ultrasonic generating part for placing the support bottom plate and the loading cover box, and performing ultrasonic processing on the test samples in the sample centrifuge tubes.

[0006] According to some embodiments, an adjusting column is arranged on the support column, and an adjusting knob is arranged outside the support column; wherein, the adjusting knob is used to adjust the height of the adjusting column based on the water level of the ultrasonic generating part, so that the relative position of the water level and the sample centrifuge tube meets the ultrasonic processing requirements.

[0007] According to some embodiments, the surface of the adjusting column is provided with scales.

[0008] According to some embodiments, the above-mentioned device further includes: a heat dissipation outer box, which is set as an unsealed box-type outer box and has an opening. The heat dissipation outer box is connected to the loading cover box for dissipating heat from the sample centrifuge tube through the opening.

[0009] According to some embodiments, the heat dissipation outer box includes: a heat dissipation box body, which is connected to the loading cover box; a semiconductor refrigeration sheet, which is arranged on the side of the heat dissipation box body connected to the loading cover box for refrigeration; a radiator, which is arranged on the side of the heat dissipation box body connected to the loading cover box for exhausting heat through the opening; a storage battery, which is electrically connected to the semiconductor refrigeration sheet and the radiator respectively for providing power for the operation of the radiator and the semiconductor refrigeration sheet; a switch button, which is arranged on the outer surface of the heat dissipation box body and is electrically connected to the storage battery for controlling the operation of the storage battery.

[0010] According to some embodiments, the refrigeration strip includes a metal strip and a heat insulation layer, and the material of the heat insulation layer is aerogel.

[0011] According to some embodiments, a plurality of concave holes are respectively arranged at the corners of the loading cover box, and a plurality of support columns are correspondingly respectively arranged at the corners of the support bottom plate.

[0012] According to some embodiments, the above-mentioned device further includes: a porous plate rack for placing the loading cover box. The porous plate rack is provided with a plurality of plate rack holes, and the plurality of plate rack holes respectively correspond to the plurality of panel holes one by one.

[0013] According to some embodiments, the diameter of the plate rack hole is the same as the diameter of the tube diameter of the sample centrifuge tube corresponding to the preset height.

[0014] According to some embodiments, the loading cover box is a polypropylene cover box, and the support bottom plate is a stainless steel plate.

[0015] It should be understood that the above general description and the following detailed description are only exemplary and do not limit the present application.

[0016] Advantageous effects:

[0017] Through the above-mentioned embodiments provided by the present application, the loading cover box loaded with the sample centrifuge tube is integrally moved and placed on the support bottom plate for ultrasonic treatment, realizing the batch transfer of samples. At the same time, the operation difficulty is low, the convenience is improved, and the problem of sample spilling caused by multiple operations of moving each sample centrifuge tube from the traditional porous plate rack to the floating plate capable of ultrasonic treatment is avoided. In addition, the multiple refrigeration strips arranged on the loading cover box can also provide a low-temperature environment for the samples in the sample centrifuge tube, which can reduce manual operation as a whole and improve the ultrasonic treatment efficiency as a whole. Description of the drawings

[0018] To more clearly illustrate the technical solutions in the embodiments of the present application, the following will briefly introduce the accompanying drawings required for the description of the embodiments. Obviously, the accompanying drawings in the following description are only some embodiments of the present application. For those of ordinary skill in the art, other accompanying drawings can also be obtained based on these drawings without exceeding the scope of protection required by the present application.

[0019] Figure 1 Schematic diagram of the application scenario provided by the embodiment of the present application;

[0020] Figure 2 Schematic diagram of the structure of the ultrasonic treatment device for experimental samples provided by the embodiment of the present application;

[0021] Figure 3 Schematic diagram of the structure of the support bottom plate provided by the embodiment of the present application;

[0022] Figure 4 Schematic diagram of the structure of the heat dissipation outer box provided by the embodiment of the present application. Detailed implementation manners

[0023] The following will clearly and completely describe the technical solutions in the embodiments of the present application with reference to the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are some, rather than all, of the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative efforts fall within the scope of protection of the present application.

[0024] Figure 1 Schematic diagram of the application scenario provided by the embodiment of the present application. In the laboratory, experimental personnel can use the ultrasonic treatment device for experimental samples to perform relevant processing on samples. In Figure 1 the application scenario shown, the support bottom plate 2 can be arranged in the ultrasonic generating part 3, and the ultrasonic generating part 3 can be provided with a mechanical moving module. Experimental personnel can place the sample centrifuge tubes containing samples that need to be ultrasonically treated in the panel holes 10 of the loading cover box 1. The mechanical moving module 5 can move the loading cover box 1 loaded with multiple sample centrifuge tubes to the support bottom plate 2, and fix the loading cover box 1 inside the ultrasonic generating part 3 through the embedded connection between the concave holes 12 and the support columns 20, and then perform ultrasonic treatment.

[0025] The specific implementation manner can refer to the following embodiments.

[0026] Figure 2 Schematic diagram of the structure of the ultrasonic treatment device for experimental samples provided by the embodiment of the present application. As Figure 2 shown, the device includes: a loading cover box 1, a support bottom plate 2, and an ultrasonic generating part 3.

[0027] The loading lid box 1 is provided with a plurality of panel holes 10 for loading sample centrifuge tubes and a plurality of refrigeration strips 11 for maintaining low temperature. A concave hole 12 is provided at the edge of the loading lid box 1, and the panel holes 10 and the refrigeration strips 11 are arranged adjacent to each other.

[0028] The support bottom plate 2 is used to support the loading lid box 1. A plurality of support columns 20 are provided at the edge of the support bottom plate 2, and the plurality of support columns 20 are respectively arranged opposite to the plurality of concave holes 12 and can be correspondingly connected to each other.

[0029] The ultrasonic generating unit 3 is used to place the support bottom plate 2 and the loading lid box 1 and perform ultrasonic treatment on the test sample in the sample centrifuge tube.

[0030] The sample centrifuge tube (Eppendorf Micro Test Tubes, EP tube) can be used to represent the centrifuge tube containing the experimental sample. The refrigeration strip 12 is replaceable.

[0031] In some implementation manners, the size of the panel holes 10 on the loading lid box 1 and the interval between two adjacent panel holes 10 can be set according to the tube diameter of the EP tube. The panel holes 10 can be symmetrically distributed on the loading lid box 1, and a plurality of refrigeration strips for maintaining low temperature can be arranged between the panel holes 10. More than or equal to four concave holes 12 can be provided at the edge of the loading lid box 1. Correspondingly, the support columns 20 corresponding to the concave holes 12 can be provided on the support bottom plate 2. The support bottom plate 2 can be fixed in the ultrasonic generating unit 3, and the ultrasonic generating unit 3 can specifically use an ultrasonic instrument.

[0032] In some other implementation manners, the concave holes 12 on the loading lid box 1 can be multiple and closely adjacent to each other.

[0033] In some other implementation manners, the planes of the loading lid box and the support bottom plate can be rectangular, and concave holes and the corresponding support columns can be provided at the four corners.

[0034] In some other implementation manners, after the ultrasonic treatment is completed, the loading lid box can be directly moved to the position where subsequent sample treatment is required and directly used as the support for the sample centrifuge tube to avoid other transfer operations.

[0035] According to the exemplary embodiment, the experimenter can place the sample centrifuge tube on the panel hole 12 of the loading lid box 1. The refrigeration strip 11 can cool the sample in the sample centrifuge tube and maintain low temperature. The experimenter can connect the concave hole 12 on the loading lid box with the support column 20 on the support bottom plate 2 so that the support bottom plate 2 can support the loading lid box 1. Then, the loading lid box 1 and the support bottom plate 2 can be integrally placed in the ultrasonic generating unit 3 for ultrasonic treatment. In some implementation manners, the support bottom plate 2 can also be fixed in the ultrasonic generating unit 3, and the loading lid box 1 can be directly placed on the support bottom plate 2 in the ultrasonic generating unit 3.

[0036] In this application, the loading lid box loaded with sample centrifuge tubes is integrally moved and placed on the support bottom plate for ultrasonic treatment, realizing the batch transfer of samples. At the same time, the operation difficulty is low, the convenience is improved, and problems such as sample spillage caused by multiple operations of moving each sample centrifuge tube from the traditional multi-well plate rack to the floating plate capable of ultrasonic treatment are avoided. In addition, multiple refrigeration strips provided on the loading lid box can also provide a low-temperature environment for the samples in the sample centrifuge tubes, which can reduce manual operation as a whole and improve the ultrasonic treatment efficiency as a whole.

[0037] Figure 3 It is a schematic structural diagram of the support bottom plate provided by the embodiment of this application. As Figure 3 shown, an adjusting column 211 is provided on the support column 20, and an adjusting knob 212 is provided outside the support column 20; wherein the adjusting knob 212 adjusts the height of the adjusting column 211 based on the water level of the ultrasonic generating part 3, so that the relative position of the water level and the sample centrifuge tube meets the requirements of ultrasonic treatment.

[0038] In some implementation manners, the support column 20 can be a hollow column, and the adjusting column 211 can be arranged inside the support column 20. By operating the adjusting knob 212, the adjusting column 211 can be raised or lowered, thereby adjusting the distance between the loading lid box 1 and the support bottom plate 2.

[0039] In some other implementation manners, one end of the adjusting column 211 can be rotatably connected to one end of the support column 20, and the adjusting column 211 can be rotated and folded to a position parallel to the support column 20. The adjusting column 211 can be controlled by the adjusting knob 212 to rise or fall.

[0040] Before ultrasonic treatment, it is necessary to determine the requirements of ultrasonic treatment. In some implementation manners, the requirements of ultrasonic treatment can be that the water level of the ultrasonic generating part 3 can submerge the position of the sample in the sample centrifuge tube.

[0041] In this application, by adjusting the adjusting knob, the height of the adjusting column is adjusted, and then the loading lid box is raised or lowered, so that the relative position of the water level of the ultrasonic generating part and the sample centrifuge tube on the loading lid box meets the requirements of ultrasonic treatment, which is convenient for ultrasonic treatment of samples. The operation method is easy to understand and improves the processing efficiency of the whole process.

[0042] According to some embodiments, a scale is provided on the surface of the adjusting column 211.

[0043] In this application, by providing a scale on the surface of the adjusting column, it is clearly determined how to adjust the adjusting knob on each support column. By ensuring the consistency of the scales, the consistency of the adjustment of the adjusting knob is guaranteed, and the problem that the loading lid box tilts due to manual adjustment, resulting in the tilting of the sample centrifuge tube and damage to the sample, etc. is avoided.

[0044] According to some embodiments, reference Figure 4 The ultrasonic processing device for the experimental sample also includes a heat dissipation outer box 4.

[0045] The heat dissipation outer box 4 is configured as a non-sealed box-type outer box having an opening. The heat dissipation outer box 4 is connected to the loading cover box 1 to dissipate heat for the sample centrifuge tube through the opening.

[0046] The heat dissipation outer box 4 may be an incompletely sealed box-type outer box, and its height may be consistent with the height of the loading box cover, so as to facilitate connection with the loading cover box 1. In some implementations, the heat dissipation outer box may be configured to have an opening on a side facing the same direction as the tube opening of the sample centrifuge tube.

[0047] The present application provides a heat dissipation outer box connected to the loading cover box, which can assist in heat dissipation of the sample centrifuge tube in the loading cover box.

[0048] According to some embodiments, reference Figure 4 The heat dissipation outer box 4 includes: a heat dissipation box body 40, a semiconductor cooling sheet 41, a radiator 42, a battery 43 and a switch button 44.

[0049] The heat dissipation box body 40 is connected to the loading cover box 1, and is used to place the semiconductor cooling sheet 41, the radiator 42, the battery 43 and the switch button 44; the semiconductor cooling sheet 41 is arranged on the side of the heat dissipation box body 40 connected to the loading cover box 1, and is used for cooling; the radiator 42 is arranged on the side of the heat dissipation box body 40 connected to the loading cover box 1, and is used for dissipating heat through opening exhaust; the battery 43 is electrically connected to the semiconductor cooling sheet 41 and the radiator 42 respectively, and is used to provide power for the operation of the radiator 42 and the semiconductor cooling sheet 41; the switch button 44 is arranged on the outer surface of the heat dissipation box body 40, and is electrically connected to the battery 43, and is used to control the operation of the battery 43.

[0050] When the sample in the sample centrifuge tube needs to be treated at low temperature, the experimenter can turn on the switch button 44 so that the battery 43 can supply power to the semiconductor refrigeration chip 41 and the radiator 42 to operate. The semiconductor refrigeration chip 41 can start cooling, so that the temperature of the sample centrifuge tube loaded on the loading cover box 1 decreases, and the radiator 42 can exhaust heat through the opening to reduce the temperature of the semiconductor refrigeration chip 41 and the temperature inside the heat dissipation box body 40, thereby providing a low temperature foundation for the loading cover box 1, so that the refrigeration strip 11 on the loading box cover 1 can maintain the low temperature.

[0051] According to some embodiments, the refrigeration strip 11 includes a metal strip (not shown in the figure) and a thermal insulation layer (not shown in the figure), and the thermal insulation layer is an aerogel thermal insulation layer.

[0052] The metal strip can be a copper pipe, which has good heat conduction performance and can quickly transfer the cold quantity to the entire loading cover box 1. The thermal insulation layer can be wrapped around the metal strip to reduce the heat exchange between the metal strip and the external environment, thereby improving the refrigeration efficiency.

[0053] In this application, the material of the thermal insulation layer can be aerogel.

[0054] Since aerogel has an extremely low thermal conductivity, lower than traditional thermal insulation materials such as polystyrene board and glass wool, it can more effectively prevent the transfer of heat and provide excellent thermal insulation effect. In addition, this material of aerogel has a lower density, and the corresponding thermal insulation layer made is lighter in weight and more environmentally friendly.

[0055] According to some embodiments, a plurality of concave holes 12 are respectively arranged at the corners of the loading cover box 1; a plurality of support columns 20 are correspondingly respectively arranged at the corners of the support bottom plate 2.

[0056] In some implementation manners, concave holes can be only arranged at the four corners of the loading cover box, and support columns are correspondingly arranged at the four corners of the support bottom plate to connect the loading cover box and the support bottom plate.

[0057] In this application, by arranging concave holes at the four corners of the loading cover box and corresponding support columns at the four corners of the support bottom plate, it is possible to avoid deformation or damage caused by excessive single-point load, which helps to maintain the flatness and stability of the loading cover box. And the four-corner support can form a stable support structure, which can resist external forces from all directions, such as lateral inertial force, longitudinal inertial force, etc., so as to maintain the stability of the panel.

[0058] According to some embodiments, the device can further include a porous plate rack (not shown in the figure).

[0059] The porous plate rack is used to place the loading cover box 1, and the porous plate rack is provided with a plurality of plate rack holes, and the plurality of plate rack holes respectively correspond to the plurality of panel holes 10 one by one.

[0060] In order to achieve convenient and batch transfer of samples, the material weight of the loading cover box cannot be too heavy, otherwise when the experimenter moves the loading cover box loaded with a large number of sample centrifugal tubes to the support bottom plate, there may be potential hazards due to the excessive weight of the loading cover box. Therefore, a layer of porous plate rack can be further arranged on the bottom plate of the loading cover box. In some implementation manners, the material weight of the porous plate rack is also relatively light. The number of plate rack holes on the porous plate rack is the same as the number of panel holes and they correspond to each other one by one.

[0061] In some other implementation manners, corresponding buckles can be arranged on the porous plate rack and the loading cover box 1.

[0062] In other implementations, the diameter of the plate rack holes on the porous plate rack can be larger than the hole diameter of the panel hole 10, and a card slot can be provided on the plate rack holes to facilitate fixing the panel holes and the sample centrifuge tubes in the panel holes 10 together.

[0063] In other implementations, the perforated plate rack may also be provided with a recessed hole 12 at the same position as that on the loading cover box 1 , so that the support column 20 on the supporting base plate 2 can connect the perforated plate rack and the loading cover box together through the recessed hole 12 .

[0064] The present application arranges a porous plate rack under the loading cover box. The number of plate rack holes on the porous plate rack is the same as the number of surface holes and corresponds one to one. This can improve the stability of the loading cover box and improve the safety of the sample centrifuge tube during placement and transfer.

[0065] According to some embodiments, the diameter of the holes in the plate rack is the same as the diameter of the sample centrifuge tube corresponding to the tube diameter at the preset height.

[0066] In some implementations, the preset height can be 2 / 3 of the height of the sample centrifuge tube, that is, the diameter of the plate rack hole can be equivalent to the diameter of the tube diameter at 2 / 3 of the height of the sample centrifuge tube. The diameter of the panel hole on the loading cover box is slightly smaller than the tube diameter at the tube mouth of the sample centrifuge tube, so that the centrifuge tube can be easily inserted through the panel hole and fall into the porous plate rack, reducing the difficulty and time of operation. In addition, the centrifuge tube can still be effectively fixed between the porous plate rack and the loading cover box 1 to prevent movement or tilting during the centrifugation process, ensuring the accuracy and safety of the experiment.

[0067] According to some embodiments, the loading cover box 1 is a panel made of polypropylene.

[0068] The present application uses a polypropylene panel as the loading cover box, which has good corrosion resistance, and avoids excessive damage to the loading cover box caused by sample dripping during the transfer of the sample to the sample centrifuge tube, thereby extending the service life of the loading cover box.

[0069] The supporting bottom plate 2 is a panel made of stainless steel.

[0070] Since the support bottom plate 2 needs to frequently work in the liquid in the ultrasonic generating part 3, for example, be placed underwater, it is necessary to ensure that the support bottom plate 2 is not easy to rust.

[0071] The present application uses a stainless steel panel as the support base plate, which can extend the service life of the support base plate.

[0072] The above has introduced the embodiments of the present application in detail. Specific examples are used herein to elaborate on the principle and implementation manner of the present application. The description of the above embodiments is only for helping to understand the method of the present application and its core idea. At the same time, any changes or deformations made by those skilled in the art based on the idea of the present application, within the specific implementation manner and application scope of the present application, fall within the scope of protection of the present application. In summary, the content of this specification should not be construed as a limitation to the present application.

Claims

1. An ultrasonic treatment device for experimental samples, characterized in that: include: A loading cover box is provided with a plurality of panel holes for loading sample centrifuge tubes and a plurality of refrigeration strips for maintaining low temperature, a plurality of concave holes are provided at the edge of the loading cover box, and the panel holes and the refrigeration strips are arranged adjacent to each other; A supporting bottom plate, used for supporting the loading cover box, wherein a plurality of supporting columns are arranged at the edge of the supporting bottom plate, and the plurality of supporting columns are respectively arranged opposite to the plurality of concave holes and can be connected to each other correspondingly; The ultrasonic generating part is used for placing the supporting bottom plate and the loading cover box, and performing ultrasonic treatment on the test sample in the sample centrifuge tube.

2. The device according to claim 1, characterized in that An adjusting column is arranged on the supporting column, and an adjusting knob is arranged outside the supporting column; Wherein, the adjusting knob is used to adjust the height of the adjusting column based on the water level of the ultrasonic generating part, so that the relative position of the water level and the sample centrifuge tube meets the ultrasonic processing requirements.

3. The device according to claim 2, characterized in that The surface of the adjusting column is provided with scales.

4. The device according to claim 1, characterized in that Also includes: The heat dissipation outer box is configured as a non-sealed box-type outer box and has an opening. The heat dissipation outer box is connected to the loading cover box to dissipate heat for the sample centrifuge tube through the opening.

5. The device according to claim 4, characterized in that The heat dissipation outer box comprises: A heat dissipation box body connected to the loading cover box; A semiconductor cooling sheet is arranged on a side where the heat dissipation box body is connected to the loading cover box, and is used for cooling; A radiator, arranged on a side where the heat dissipation box body is connected to the loading cover box, and used for exhausting and dissipating heat through the opening; A storage battery, electrically connected to the semiconductor cooling sheet and the radiator, respectively, for providing power for the radiator and the semiconductor cooling sheet; A switch button is arranged on the outer surface of the heat dissipation box body and is electrically connected to the battery to control the operation of the battery.

6. The device according to any one of claims 1 to 5, characterized in that: The refrigeration strip comprises a metal strip and a thermal insulation layer, and the thermal insulation layer is an aerogel thermal insulation layer.

7. The device according to claim 1, characterized in that The plurality of concave holes are respectively arranged at the corners of the loading cover box, and the plurality of supporting columns are correspondingly respectively arranged at the corners of the supporting bottom plate.

8. The device according to claim 1, characterized in that Also includes: A multi-hole plate rack is used to place the loading cover box, and the multi-hole plate rack is provided with a plurality of plate rack holes, and the plurality of plate rack holes respectively correspond to the plurality of panel holes one by one.

9. The device according to claim 8, characterized in that The diameter of the plate rack hole is the same as the diameter of the sample centrifuge tube corresponding to the tube diameter at a preset height.

10. The device according to any one of claims 1 to 5, characterized in that: The loading cover box is a polypropylene cover box, and the supporting bottom plate is a stainless steel plate.

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