Rat fixing device in AWR behavioral test

Abstract

The utility model discloses a rat fixing device in an AWR (abdominal wall withdrawal reflex) behavioral test, belongs to the technical field of medical equipment, and aims to solve the problem of poor effect of keeping a rat in a static state in the AWR behavioral test in the prior art. Comprising a box body, one side of the box body is open, a limiting plate is installed on the side face of the upper end of the open side of the box body, a groove is formed in the side, close to the box body, of the limiting plate, a baffle is slidably clamped in the groove, and multiple sets of evenly-distributed fixing assemblies are installed at the lower end of the side face of the box body. According to the rat fixing device in the AWR behavioral test, through the arrangement of the box body, a rat is preliminarily limited and prevented from running off, through the arrangement of the fixing assembly, the rat is conveniently fixed, the static state of the rat can be ensured, the effect of keeping the static state is good, then the rat is conveniently observed, the adjustability is good, and the rat fixing device has good body adaptability and sensitivity; the method has important scientific research significance.

Description

Technical Field

[0001] The utility model belongs to the technical field of medical experimental equipment, and particularly relates to a fixing device for rats in AWR behavioral tests. Background Technique

[0002] Visceral hypersensitivity plays an important role in clinical disease detection, such as diseases like IBS and anorectal stricture. Currently, interventional medical device technologies such as balloon dilation have been well applied clinically, but the devices used in basic research are limited. Until 2000, Al-Chaer et al. induced visceral hypersensitivity by repeated colorectal distension (CRD) stimulation and established a new and better chronic visceral pain hypersensitivity model (IBS rat model). Currently, CRD can not only simulate visceral pain hypersensitivity in some clinical functional or organic intestinal diseases but also be used to detect intestinal sensitivity.

[0003] In experiments, since the detection of visceral pain hypersensitivity is manifested in the pressure value generated by the extrusion of the intestinal wall by balloon dilation, and the observation of the rats' abdominal lifting due to visceral pain during the test depends on the static state of the rats. Currently, researchers have a wide variety of choices for rat fixation methods. For example, they let the rats get into gloves and hold the rats' heads by hand, but there are disadvantages such as causing harm to the rats and the rats strongly resisting and terminating the experiment; or they make a transparent box that fits the size of the rats and let them get into it for fixation. However, letting the rats get into a closed space does not make them stay still well. Due to their curious nature, the rats will explore the closed space, increasing the difficulty for observers to observe the rats' abdominal lifting due to visceral pain. Moreover, the fixation boxes used by scientific researchers are often self-made with poor effects. Therefore, a technical measure is proposed to solve the problem that the rats have a poor effect of maintaining a static state in AWR (abdominal wall withdrawal reflex) behavioral tests under the existing technology. Content of the Utility Model

[0004] Aiming at the deficiencies of the existing technology, the purpose of the utility model is to provide a fixing device for rats in AWR behavioral tests, aiming to solve the problem that the rats have a poor effect of maintaining a static state in AWR (abdominal wall withdrawal reflex) behavioral tests under the existing technology.

[0005] To solve the above technical problems, the present utility model provides a rat fixing device in AWR behavioral testing, which includes a box body. One side of the box body is provided with an opening. A limiting plate is installed on the upper side of the side where the box body has the opening. A groove is formed on the side of the limiting plate close to the box body. A baffle is slidably engaged in the groove. A plurality of groups of evenly distributed fixing components are installed at the lower end of the side of the box body. Thanks to the setting of the box body, the rats are preliminarily limited to prevent them from running away. Thanks to the setting of the fixing components, it is convenient to fix the rats, which can ensure the static state of the rats, and has a good effect of maintaining the static state. Furthermore, it is convenient to observe the rats, has good adjustability, has good body adaptability and sensitivity, and has important scientific research significance.

[0006] Furthermore, a plurality of groups of evenly distributed air holes are formed at the bottom of the box body. A pulling plate is installed at the central position of the upper surface of the baffle, and the pulling plate is in contact with the upper surface of the limiting plate.

[0007] Furthermore, the box body and the baffle are made of acrylic material. The anus of the rat is connected with a visceral pain measurement device. The catheter of the rat's tail and the visceral pain measurement device passes through the gap between the baffle and the box body. Thanks to the setting of the acrylic material of the box body, it has good transparency and is convenient to observe the state of the rats.

[0008] Furthermore, the fixing component includes a box body. There are a plurality of groups of the box bodies, and the box bodies are fixedly installed at the lower end of the side of the box body. A threaded hole is formed at the central position of the upper surface of the box body, and a screw rod is threadedly connected to the threaded hole. The upper end of the screw rod is connected with a knob, the lower end of the screw rod is rotatably connected with a lifting block, a rubber pad is connected to the lower surface of the lifting block, a side hole is formed at the lower end of the side of the box body, and a connecting rod is installed at the central position below the inside of the box body, and the upper end of the connecting rod is connected with a limiting block.

[0009] Furthermore, a limiting hole is formed on the upper surface of the limiting block, and a through hole is formed on the side of the limiting block. The limiting hole is slidably connected with the lifting block and the rubber pad, and the limiting hole provides a limiting force for the lifting block and the rubber pad. Thanks to the setting of the rubber pad, the friction is increased, the fixing effect is improved, and the rigid contact is prevented from damaging the pulling rope.

[0010] Even further, a pulling rope passes through the through hole and the side hole movably, and the sizes of the through hole and the side hole are larger than the pulling rope. One end of the pulling rope is connected with a pulling head, and the other end of the pulling rope is fixedly installed at the lower end of the inner side of the box body, and the fixed position is adapted to the box body. A plurality of groups of magnetic plates are installed outside the pulling rope.

[0011] Even further, the distance between the magnetic plates is adapted to the height and width of the box body. The magnetic plates magnetically attract iron plates. There are a plurality of groups of the iron plates, and the plurality of groups of iron plates are fixedly installed on both sides of the upper and lower surfaces inside the box body.

[0012] Compared with the prior art, the beneficial effects of the utility model are as follows:

[0013] Through the setting of the box body, the rat is initially limited to prevent it from running away, and the rat is driven into the interior of the box body. When the rat enters the interior of the box body, it passes through multiple groups of pull ropes.

[0014] Through the setting of the fixing component, it is convenient to fix the rat, which can ensure the static state of the rat, has a good effect of maintaining the static state, and then is convenient for observing the rat. It has good adjustability, good body adaptability and sensitivity, and has important scientific research significance. Pull the pull head in the middle position to release the magnetic attraction between the magnetic plate and the iron plate, so that the pull rope binds the rat tightly. Then rotate the knob to drive the rubber pad to move downward and press tightly against the pull rope, so that the pull rope is fixed. At this time, the middle position of the rat is fixed. Then use another pull rope to fix the front and hind limbs of the rat. After the fixation is completed, insert the visceral pain measurement device into the rat's body. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present application. For those skilled in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0016] Figure 1 It is a schematic structural diagram of the utility model;

[0017] Figure 2 It is a schematic bottom view structure diagram of the box body;

[0018] Figure 3 It is a schematic structure diagram of the limiting plate and the baffle;

[0019] Figure 4 It is a schematic structure diagram of the fixing component;

[0020] Figure 5 It is a schematic structure diagram of the separated state of the box body, the screw rod and the limiting block.

[0021] In the drawings: 1. Box body; 2. Fixing component; 3. Baffle; 4. Air hole; 5. Pulling plate; 6. Limiting plate; 7. Groove; 201. Box body; 202. Pull rope; 203. Magnetic plate; 204. Iron plate; 205. Pull head; 206. Threaded hole; 207. Knob; 208. Screw rod; 209. Limiting block; 210. Limiting hole; 211. Rubber pad; 212. Connecting rod; 213. Side hole; 214. Through hole; 215. Lifting block. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0022] The following will clearly and completely describe the technical solutions in the embodiments of the present utility model with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the protection scope of the present utility model.

[0023] This specific embodiment is a fixing device for rats in the AWR behavioral test. The schematic structural diagram is as Figure 1 、 Figure 2 、 Figure 3 shown, including a box body 1. One side of the box body 1 is provided with an opening. A limiting plate 6 is installed on the upper side surface of the opening side of the box body 1. A groove 7 is opened on the side of the limiting plate 6 close to the box body 1. A baffle 3 is slidably engaged in the groove 7. A plurality of groups of evenly distributed fixing components 2 are installed on the lower side of the box body 1. A plurality of groups of evenly distributed air holes 4 are opened at the bottom of the box body 1. A pull plate 5 is installed at the center position of the upper surface of the baffle 3. The pull plate 5 is in contact with the upper surface of the limiting plate 6. The box body 1 and the baffle 3 are made of acrylic material. The anus of the rat is connected with a visceral pain measurement device. The tail of the rat and the catheter of the visceral pain measurement device pass through the gap between the baffle 3 and the box body 1. When actually conducting the AWR behavioral test on the rat, pull up the pull plate 5 to drive the baffle 3 to move upward. When the baffle 3 completely exposes the inside of the box body 1, select a rat that matches the size of the box body 1, and then drive the rat into the box body 1. After the rat enters the box body 1, use the fixing components 2 to fix the rat. After the fixing is completed, insert the visceral pain measurement device into the rat's body, and release the pull plate 5. The baffle 3 drops and contacts the catheter of the pain measurement device and the tail of the rat.

[0024] Refer to Figure 1 、 Figure 4 、 Figure 5As shown in the figure, the fixing component 2 includes a box body 201. There are multiple groups of box bodies 201, and the box bodies 201 are fixedly installed at the lower end of the side surface of the box body 1. A threaded hole 206 is provided at the center of the upper surface of the box body 201. A screw rod 208 is threadedly connected to the threaded hole 206. The upper end of the screw rod 208 is connected with a knob 207. The lower end of the screw rod 208 is rotatably connected with a lifting block 215. A rubber pad 211 is connected to the lower surface of the lifting block 215. A side hole 213 is provided at the lower end of the side surface of the box body 201. A connecting rod 212 is installed at the center of the lower part inside the box body 201. The upper end of the connecting rod 212 is connected with a limiting block 209. A limiting hole 210 is provided on the upper surface of the limiting block 209. A through hole 214 is provided on the side surface of the limiting block 209. The limiting hole 210 is slidably connected with the lifting block 215 and the rubber pad 211, and the limiting hole 210 provides a limiting force for the lifting block 215 and the rubber pad 211. A pull rope 202 (or a cable tie made of nylon plastic with a length of 20 cm) is movably passed through the through hole 214 and the side hole 213, and the sizes of the through hole 214 and the side hole 213 are larger than the pull rope 202. One end of the pull rope 202 is connected with a pull head 205. The other end of the pull rope 202 is fixedly installed at the lower end of the inner side surface of the box body 1, and the fixed position is adapted to the box body 201. Multiple magnetic plates 203 are installed outside the pull rope 202. The distance between the magnetic plates 203 is adapted to the height and width of the box body 1. The magnetic plates 203 magnetically attract iron plates 204. There are multiple groups of iron plates 204, and multiple groups of iron plates 204 are fixedly installed on both sides of the upper and lower surfaces inside the box body 1. Pull the pull head 205 at the middle position. The pull head 205 drives the pull rope 202 to move along the through hole 214 and the side hole 213. The movement of the pull rope 202 causes the magnetic plates 203 to release the magnetic attraction with the iron plates 204, thereby making the pull rope 202 tighten the rat. Then rotate the knob 207. The rotation of the knob 207 drives the screw rod 208 to rotate downward. The downward movement of the screw rod 208 drives the lifting block 215 to move downward along the limiting hole 210. The downward movement of the lifting block 215 drives the rubber pad 211 to move downward to press against the pull rope 202, so that the pull rope 202 is fixed. At this time, the middle position of the rat is fixed. Then use another pull rope 202 to fix the front and rear limbs of the rat.

[0025] Working principle: When actually performing the AWR ethology test on the rat, pull the pull plate 5 upward to drive the baffle 3 to move upward. When the baffle 3 completely exposes the inside of the box body 1 (the size of the box body 1 is 20 cm * 6 cm * 8 cm, and there is a gap between the baffle 3 and the box body 1), select a rat that matches the size of the box body 1 (when the size of the rat matches the box body 1, the fixing component 2 can fix the front limb (scapular cross-section), back (10th thoracic vertebra cross-section) and hind limb part (hind limb cross-section) of the rat). Then drive the rat into the inside of the box body 1. After the rat enters the inside of the box body 1 (the head of the rat faces away from the side of the baffle 3), use the fixing component 2 to fix the rat. Through the setting of the box body 1, the rat is initially limited to prevent it from running away.

[0026] The specific fixing method is to pull the slider 205 at the middle position. The slider 205 drives the pull rope 202 to move along the through hole 214 and the side hole 213. The movement of the pull rope 202 causes the magnetic plate 203 to release the magnetic attraction with the iron plate 204, and then the pull rope 202 binds the rat tightly. Subsequently, rotate the knob 207. The rotation of the knob 207 drives the screw rod 208 to rotate downward. The downward movement of the screw rod 208 drives the lifting block 215 to move downward along the limiting hole 210 (the limiting hole 210 provides a limiting force for the lifting block 215 to prevent the lifting block 215 from deflecting). The downward movement of the lifting block 215 drives the rubber pad 211 to move downward to press against the pull rope 202 (the rubber pad 211 increases the friction force, improves the fixing effect, and prevents damage to the pull rope 202 caused by rigid contact), so that the pull rope 202 is fixed. At this time, the middle position of the rat is fixed (the rat needs to be fixed at three places: the forelimb, the back, and the hindlimb). Subsequently, use another pull rope 202 to fix the forelimbs and hindlimbs of the rat. After the fixing is completed, insert the visceral pain detection device into the rat's body. Release the pull plate 5, and the baffle 3 drops to contact the catheter of the pain detection device and the rat's tail. After the test is completed, release the limit on the pull rope 202, and magnetically attract the magnetic plate 203 on the pull rope 202 to the iron plate 204, so that the pull rope 202 is completely opened, facilitating the entry of the rat. Through the setting of the fixing assembly 2, it is convenient to fix the rat, can ensure the static state of the rat, is convenient for observing the rat, has good adjustability, has good adaptability and sensitivity to the body, and has important scientific research significance.

[0027] Visceral nociception detection:

[0028] Rats were fasted for 18 hours before the experiment with free access to water, while mice were not fasted or water-deprived. During the experiment, after the large / small mice had adapted to the environment for several minutes, they were allowed to crawl into the glove. The body and tail of the mouse were fixed with the left hand, leaving the tail at the end of the glove opening. After pushing the syringe to expand the balloon with the right hand, the balloon tip was dipped in an appropriate amount of lubricant (such as paraffin oil, glycerin, vaseline, and sesame oil), the tail was gently lifted to expose the anus, and the anus was gently wiped back and forth to induce defecation in the large / small mice. After defecation was completed, the syringe was pulled back to deflate the balloon, and then the balloon tube with lubricant was slowly inserted into the anus with a sudden force. When the balloon and the tube were inserted to the end and the ligation line was about 1 cm from the anal sphincter in rats and about 0.5 cm in mice, the outer end of the catheter on the tail was gently fixed to the mouse tail with the cut tape, with a slight tension to prevent the balloon from falling off. After removing the glove, the rat was placed in an acrylic box 1 with dimensions of 20 cm * 6 cm * 8 cm for overall fixation, leaving the tail and the catheter device outside the acrylic box, and then the rat was locally fixed with a cable tie in an appropriate size. After the large / small mice became quiet again, pressure was continuously and slowly applied through the syringe, and the minimum pressure value when obvious abdominal muscle contraction and flattening or the lower abdominal wall lifting off the table were observed with the naked eye was taken as the colorectal pain response threshold of the large / small mice. Some experiments have shown that an expansion pressure value greater than 80 mmHg may cause damage to the colorectal mucosa of large / small mice, so the expansion pressure range should be maintained between 10 mmHg and 80 mmHg; each rat was repeated three times and the average value was taken.

[0029] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A rat fixation device in an AWR behavioral test, comprising a box body (1), characterized in that, One side of the box body (1) is provided with an opening. A limit plate (6) is installed on the upper side surface of the opening side of the box body (1). A groove (7) is formed on the side of the limit plate (6) close to the box body (1). A baffle plate (3) is slidably engaged in the groove (7). A plurality of uniformly distributed fixing components (2) are installed at the lower end of the side surface of the box body (1).

2. The rat fixation device in an AWR behavioral test according to claim 1, characterized in that, A plurality of uniformly distributed air holes (4) are formed at the bottom of the box body (1). A pull plate (5) is installed at the center position of the upper surface of the baffle plate (3). The pull plate (5) is in contact with the upper surface of the limit plate (6).

3. The rat fixation device in an AWR behavioral test according to claim 1, wherein, The box body (1) and the baffle plate (3) are made of acrylic material. The anus of the rat is connected with a visceral pain measuring device. The catheter of the rat's tail and the visceral pain measuring device passes through the gap between the baffle plate (3) and the box body (1).

4. The rat fixation device in an AWR behavioral test according to claim 1, characterized in that, The fixing component (2) includes a box body (201). There are a plurality of box bodies (201). The box bodies (201) are fixedly installed at the lower end of the side surface of the box body (1). A threaded hole (206) is formed at the center position of the upper surface of the box body (201). A screw rod (208) is threadedly connected to the threaded hole (206). The upper end of the screw rod (208) is connected with a knob (207). The lower end of the screw rod (208) is rotatably connected with a lifting block (215). A rubber pad (211) is connected to the lower surface of the lifting block (215). A side hole (213) is formed at the lower end of the side surface of the box body (201). A connecting rod (212) is installed at the center position below the inside of the box body (201). The upper end of the connecting rod (212) is connected with a limit block (209).

5. The rat fixation device in an AWR behavioral test according to claim 4, wherein A limit hole (210) is formed on the upper surface of the limit block (209). A through hole (214) is formed on the side surface of the limit block (209). The limit hole (210) is slidably connected with the lifting block (215) and the rubber pad (211), and the limit hole (210) provides a limiting force for the lifting block (215) and the rubber pad (211).

6. The rat fixation device in an AWR behavioral test according to claim 5, characterized in that A pull rope (202) passes through the through hole (214) and the side hole (213) movably, and the sizes of the through hole (214) and the side hole (213) are larger than the pull rope (202). One end of the pull rope (202) is connected with a pull head (205). The other end of the pull rope (202) is fixedly installed at the lower end of the inner side surface of the box body (1), and the fixed position is adapted to the box body (201). A plurality of magnetic plates (203) are installed outside the pull rope (202).

7. The rat fixation device in the AWR behavioral test according to claim 6, characterized in that, The distance between the magnetic plates (203) is adapted to the height and width of the box body (1). The magnetic plates (203) magnetically attract iron plates (204). There are a plurality of iron plates (204). A plurality of the iron plates (204) are fixedly installed on both sides of the upper and lower surfaces inside the box body (1).

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