Sample treatment and detection integrated device

Abstract

The utility model discloses a sample treatment and detection integrated device, and belongs to the field of medical examination and inspection instruments and molecular diagnosis and detection instruments. According to the technical key points, the system comprises a system outer cover assembly, a loading round chassis assembly and a rotary liquid transfer device assembly, the object-carrying circular chassis assembly comprises an object-carrying circular chassis; a waste liquid bin, a magnetic bead lysis solution bin, a reagent tube, a PCR tube and a washing liquid bin are uniformly arranged on the loading round chassis; an insertion cylinder is further arranged in the middle of the loading round base plate; the rotary liquid transfer device assembly comprises a rotary liquid transfer device; the offset pipette comprises an upper through pipe interface, a lower pipe interface and an offset pipe; the lower portion of the first vertical through pipe is connected with the upper through pipe connector, and the upper portion of the second vertical pipe is connected with the lower through pipe connector. The first vertical through pipe and the second vertical pipe are located on the same vertical axis, and the first vertical through pipe is communicated with the offset pipe.

Description

Technical Field

[0001] This application relates to the field of medical inspection and testing instruments and molecular diagnostic testing instruments. More specifically, it particularly relates to an integrated device for sample processing and detection. Background Art

[0002] CN220284078U discloses an integrated norovirus magnetic bead fluorescence detection cartridge, which can centrally process three processes of nucleic acid extraction, purification, and fluorescence PCR detection, thereby improving the detection efficiency of pathogens and reducing the requirements for testers.

[0003] However, the above detection cartridge has the following problems:

[0004] (1) The operation process cannot meet the requirement of full enclosure. During the operation process, when the pipette tip contacts the required liquid storage tank, it is necessary to open the sealing cover above the liquid storage tank, and at this time, the liquid storage tank will contact the air, causing contamination.

[0005] (2) During the operation process, each time liquid is aspirated and discharged, it is necessary to open and close the corresponding sealing cover once, which results in low operation efficiency. Utility Model Content

[0006] The purpose of this application is to provide an integrated device for sample processing and detection in view of the above-mentioned deficiencies of the prior art.

[0007] The technical solution of this application is as follows:

[0008] An integrated device for sample processing and detection, which includes: a system outer cover assembly, a load-carrying circular chassis assembly, and a rotary pipettor assembly;

[0009] Among them, the upper surface of the outer cover body is provided with a sample addition port, a pressure balance port, and a pipettor barrel, and its lower part is provided with an opening;

[0010] Among them, the load-carrying circular chassis assembly includes: a load-carrying circular chassis; the following are evenly placed on the load-carrying circular chassis: a waste liquid bin, a magnetic bead lysis solution bin, reagent tubes, PCR tubes, and a washing solution bin; the distances from the centers of the waste liquid bin, the magnetic bead lysis solution bin, the reagent tubes, the PCR tubes, and the washing solution bin to the center of the load-carrying circular chassis are r;

[0011] An insertion cylinder is further provided in the middle of the load-carrying circular chassis;

[0012] Among them, the rotary pipette assembly includes: a rotary pipette; the rotary pipette includes: a first vertical through-tube, a second vertical tube, an offset pipette tube, and a bottom limiter; the offset pipette tube includes: an upper through-tube interface, a lower tube interface, and an offset tube; the lower part of the first vertical through-tube is connected to the upper through-tube interface, and the upper part of the second vertical tube is connected to the lower tube interface; the first vertical through-tube and the second vertical tube are on the same vertical axis, and the first vertical through-tube communicates with the offset tube; the offset tube is arranged parallel to the second vertical tube and the axial distance between the two is also r;

[0013] Among them, the load-carrying circular chassis is hermetically connected to the lower opening of the outer cover body;

[0014] Among them, the top of the first vertical through-tube passes through the pipette barrel, the second vertical tube and the bottom limiter are inserted into the insertion cylinder, and the outer diameter of the bottom limiter is adapted to the inner diameter of the insertion cylinder.

[0015] Furthermore, the bottom limiter is a cylinder.

[0016] Furthermore, the sample addition port corresponds to the magnetic bead lysis solution chamber.

[0017] Furthermore, there is an air pump adapter at the upper part of the first vertical through-tube, and the upper part of the first vertical through-tube is connected to an air pump.

[0018] Furthermore, a silica gel gasket is also provided between the first vertical through-tube and the air pump adapter.

[0019] Furthermore, an anti-aerosol filter element is provided in the upper through-tube interface.

[0020] Furthermore, the number of magnetic bead lysis solution chambers is 1, and lysis solution is pre-stored in the magnetic bead lysis solution chamber.

[0021] Furthermore, the number of waste liquid chambers is 1.

[0022] Furthermore, the number of washing liquid chambers is 3, and washing liquid is pre-stored in all of them.

[0023] Furthermore, the number of reagent tubes is 3, and eluent, eluent, and paraffin liquid are pre-stored respectively.

[0024] Furthermore, the number of PCR tubes is 5, and freeze-dried balls are pre-stored respectively.

[0025] The beneficial effects of this application are as follows:

[0026] (1) This application is provided with a system outer cover assembly. The system outer cover assembly is hermetically connected to the load-carrying circular chassis through snap fasteners. At the same time, the following are placed on the load-carrying circular chassis: a waste liquid tank, a magnetic bead lysis solution tank, reagent tubes, PCR tubes, and a washing liquid tank. Through the design of the system outer cover assembly, the openings of the waste liquid tank, the magnetic bead lysis solution tank, the reagent tubes, the PCR tubes, and the washing liquid tank are in the internal space of the system outer cover assembly, avoiding their contamination.

[0027] (2) After the sample is added, the integrated device of this application realizes sample processing through the suction, up and down movement, and 360-degree rotation of the rotary pipettor, controls the temperature of the PCR tube and conducts nucleic acid amplification reaction, detects the amplification result through fluorescence detection, completes fully enclosed nucleic acid extraction and detection, has a high degree of automation, and has a simple structure, reducing the probability of sample contamination and improving the accuracy of sample processing and detection. Brief Description of the Drawings

[0028] The following further elaborates on this application with reference to the embodiments in the drawings, but does not constitute any limitation to this application.

[0029] Figure 1 It is a three-dimensional design schematic diagram of the integrated device for sample processing and detection of this application.

[0030] Figure 2 It is a three-dimensional design schematic diagram of the outer cover body of this application.

[0031] Figure 3 It is a three-dimensional exploded design schematic diagram of the integrated device for sample processing and detection of this application.

[0032] Figure 4 It is a three-dimensional design schematic diagram of the load-carrying circular chassis assembly of this application.

[0033] Figure 5 It is a design schematic diagram of the load-carrying circular chassis assembly from another perspective of this application.

[0034] Figure 6 It is a design schematic diagram of the lower surface of the load-carrying circular chassis of this application.

[0035] Figure 7 It is a structural relationship diagram of the rotary pipettor assembly and the load-carrying circular chassis assembly of this application.

[0036] Figure 8 It is a sectional design schematic diagram of the integrated device for sample processing and detection of this application.

[0037] Figure 9 It is an elevation schematic diagram of the integrated device for sample processing and detection of this application.

[0038] Figure 10It is the numbered diagram of the functional compartment of the load-carrying round chassis assembly of this application.

[0039] The description of the reference numerals is as follows:

[0040] Sample processing and detection integrated device 1000;

[0041] System outer cover assembly 2000, outer cover body 2100, sample addition port 2101, air pressure balance port 2102, pipettor barrel 2103, sample addition port cover 2201, silicone seal 2202, sealing ring 2301, pressing cover 2302, air pressure balance filter element 2400;

[0042] Load-carrying round chassis assembly 3000, load-carrying round chassis 3100, waste liquid bin 3300, magnetic bead lysis solution bin 3400, 3-row reagent tube 3500, 5-row PCR tube 3600, washing liquid bin 3700;

[0043] Rotary pipettor assembly 4000, rotary pipettor 4100, first vertical through tube 4101, second vertical tube 4102, offset pipette 4103, bottom limiter 4104, air pump adapter 4200, silicone gasket 4300, anti-aerosol filter element 4400. Detailed implementation manners

[0044] Although various embodiments of this application have been shown and described herein, it will be apparent to those skilled in the art that such embodiments are provided by way of example only. Without departing from this application, those skilled in the art can conceive of many variations, changes, and substitutions. It should be understood that various alternative embodiments of the invention described herein can be adopted.

[0045] Embodiment 1: A sample processing and detection integrated device

[0046] As Figure 1 shown, the sample processing and detection integrated device 1000 includes: a system outer cover assembly 2000, a load-carrying round chassis assembly 3000, and a rotary pipettor assembly 4000.

[0047] <Structural description of the system outer cover assembly 2000>

[0048] As Figure 2 and Figure 3 shown, the system outer cover assembly 2000 includes: an outer cover body 2100, a sample addition port cover 2201, a silicone seal 2202, a sealing ring 2301, a pressing cover 2302, and an air pressure balance filter element 2400;

[0049] The upper surface of the outer cover body 2100 is provided with a sample addition port 2101, an air pressure balance port 2102, and a pipettor barrel 2103;

[0050] The silicone seal 2202 can be inserted into the sample loading port 2101, and the sample loading port cover 2201 and the silicone seal 2202 can seal the sample loading port 2101.

[0051] The sealing ring 2301 is arranged inside the pipette barrel 2103, and the pressing cover 2302 is arranged outside the pipette barrel 2103.

[0052] The air pressure balance port 2102 is provided with the air pressure balance filter element 2400, and through the air pressure balance filter element 2400, foreign matters in the outside are prevented from entering the interior of the integrated sample processing and detection device 1000.

[0053] <Description of the structure of the load-carrying round chassis assembly 3000>

[0054] As Figure 3 shown, the load-carrying round chassis assembly 3000 includes: a load-carrying round chassis 3100, 1 waste liquid tank 3300, 1 magnetic bead lysis solution tank 3400, 1 three-row reagent tube 3500, 1 five-row PCR tube 3600, and 3 washing liquid tanks 3700.

[0055] From Figure 4 and Figure 6 it can be seen that: the load-carrying round chassis 3100 includes: a load-carrying round chassis body 3101 and an insertion tube 3102 arranged at the central part of the load-carrying round chassis 3100; in addition, the following are arranged in the circumferential direction of the load-carrying round chassis body 3101: 1 waste liquid tank connection 3103, 1 magnetic bead lysis solution tank connection port 3104, 3 reagent tube connection ports 3105, 5 PCR tube connection ports 3106, and 3 washing liquid tank connection ports 3107.

[0056] The magnetic bead lysis solution tank connection port 3104 corresponds to the connection of the magnetic bead lysis solution tank 3400; 3 of the reagent tube connection ports 3105 correspond to the connection of the three-row reagent tube 3500; 5 of the PCR tube connection ports 3106 correspond to the connection of the five-row PCR tube 3600; the washing liquid tank connection port 3107 corresponds to the connection of the washing liquid tank 3700.

[0057] A waste liquid tank silicone cover 3800 is pasted above the waste liquid tank 3103.

[0058] <Description of the structure of the rotary pipette assembly 4000>

[0059] From Figure 3 , Figure 7 and Figure 8 it can be seen that: the rotary pipette assembly 4000 includes: a rotary pipette 4100, an air pump adapter 4200, a silicone gasket 4300, and an anti-aerosol filter element 4400;

[0060] The rotary pipettor 4100 includes: a first vertical through-tube 4101, a second vertical tube 4102, an offset pipette tube 4103, and a bottom limiter 4104;

[0061] An air pump adapter 4200 is installed at the upper part of the first vertical through-tube 4101, and a silicone gasket 4300 is further provided between the upper part of the first vertical through-tube 4101 and the air pump adapter 4200 to prevent the inside of the rotary pipettor 4100 from being contaminated before use.

[0062] The offset pipette tube 4103 includes: an upper through-tube interface, a lower tube interface, and an offset tube; the first vertical through-tube 4101 is connected to the upper through-tube interface, and the second vertical tube 4102 is connected to the lower tube interface; the first vertical through-tube 4101 is in communication with the offset tube; the upper part of the second vertical tube 4102 is closed and is not in communication with the first vertical through-tube 4101.

[0063] An anti-aerosol filter element 4400 is provided in the upper through-tube interface to prevent the offset pipette tube 4103 from being contaminated by outside air.

[0064] A bottom limiter 4104 is provided at the bottom of the second vertical tube 4102.

[0065] The working principle of the rotary pipettor assembly 400 is as follows:

[0066] a. Liquid suction: The rotary pipettor moves downward, so that the offset pipette tube enters the function chamber of the carrier round chassis. The air pump adapter at the upper part of the first vertical through-tube is connected to an air pump, and through the suction work of the air pump, the solution in the function chamber is thus sucked into the offset pipette tube.

[0067] b. Solution transfer: The rotary pipettor moves upward and rotates the rotary pipettor so that the offset tube reaches a preset position.

[0068] c. Liquid discharge: The rotary pipettor moves downward, so that the offset pipette tube enters the function chamber of the carrier round chassis. The air pump adapter at the upper part of the first vertical through-tube is connected to an air pump, and through the exhalation work of the air pump, the solution in the offset pipette tube is thus discharged into the function chamber.

[0069] <Description of the Structural Matching Design of the System Enclosure Assembly, the Carrier Round Chassis Assembly, and the Rotary Pipettor Assembly>

[0070] (1) The carrier round chassis 3100 is inserted into the lower opening of the enclosure body 2100.

[0071] (2) The top of the first vertical through-tube 4101 passes through the pipette barrel 2103, the second vertical tube 4102 and the bottom limiter 4104 are inserted into the insertion cylinder 3102, and the outer diameter of the bottom limiter 4104 is adapted to the inner diameter of the insertion cylinder 3102.

[0072] (3) The vertical central axes of the first vertical through-tube 4101, the second vertical tube 4102, and the bottom limiter 4104 are the same axis.

[0073] (4) The sample loading port 2101 corresponds to the magnetic bead lysis solution chamber 3400.

[0074] For the convenience of description, one magnetic bead lysis solution chamber 3400, one 3-row reagent tube 3500, one 5-row PCR tube 3600, three washing solution chambers 3700, and one waste liquid chamber 3300 on the load-carrying round chassis are numbered in the foregoing order as: holes No. 1 to No. 13.

[0075] According to the foregoing sample processing and detection integrated device 1000, its operation method is as follows:

[0076] S100, Place the sample: Open the sample loading port cover 2201 and the silica gel seal 2202, and place the sample into hole No. 1 (i.e., the magnetic bead lysis solution chamber 3400 is pre-stored with lysis solution and magnetic beads).

[0077] S200, Transfer the remaining waste liquid in hole No. 1 to hole No. 13 (i.e., the waste liquid chamber); it includes the following sub-steps:

[0078] S201, Place a magnet at the bottom of the magnetic bead lysis solution chamber to magnetically attract the magnetic beads, and let the magnetic beads adhere to the wall for 1 minute;

[0079] S202, Rotate the rotary pipette 4100 so that the offset tube is aligned with hole No. 1 (i.e., the magnetic bead lysis solution chamber), lower the rotary pipette 4100 to aspirate the liquid, and after the liquid aspiration is completed, raise the rotary pipette 4100;

[0080] S203, Rotate the rotary pipette 4100 so that the offset tube is aligned with hole No. 13 (i.e., the waste liquid chamber), lower the rotary pipette 4100 to drain the liquid, and after the liquid drainage is completed, raise the rotary pipette 4100;

[0081] S204, Demagnetize: That is, remove the magnet placed at the bottom of the magnetic bead lysis solution chamber;

[0082] S300, Wash 3 times (wash the impurity molecules such as proteins attached to the magnetic beads), and recycle the waste liquid to the original test tube; it includes the following sub-steps:

[0083] S301. Rotate the rotary pipettor 4100 so that the offset tube is aligned with hole No. 10 (i.e., the washing liquid chamber), lower the rotary pipettor 4100 so that the offset tube sucks the washing liquid, and then raise the rotary pipettor 4100 after sucking (complete transfer);

[0084] S302. Rotate the rotary pipettor 4100 so that the offset tube is aligned with hole No. 1 (i.e., the magnetic bead lysis solution chamber), lower the rotary pipettor 4100 to drain the liquid, blow and mix 20 times, and then raise the rotary pipettor 4100 after completion;

[0085] S303. Place a magnet at the bottom of the magnetic bead lysis solution chamber to magnetize the magnetic beads, and let the magnetic beads adhere to the wall for 1 minute;

[0086] S304. Lower the rotary pipettor 4100, suck the waste liquid, and then raise it;

[0087] S305. Rotate the rotary pipettor 4100 so that the offset tube is aligned with hole No. 10 (i.e., the washing liquid chamber), and lower it to drain the liquid;

[0088] S306. Remove the magnet;

[0089] The above S301 - S306 is one - time washing, and then wash the magnetic beads twice with the washing liquid in hole No. 11 and hole No. 12;

[0090] S400. Heat the magnetic bead lysis solution chamber 3400, and dry the magnetic beads at 56 °C for 300 seconds;

[0091] S500. Rotate the rotary pipettor 4100 so that the offset tube is aligned with hole No. 3, lower the rotary pipettor 4100 and blow - suck 20 times (clean the pipette tip), and then raise the rotary pipettor 4100 after completion;

[0092] S600. Rotate the rotary pipettor 4100 so that the offset tube is aligned with hole No. 2, lower the rotary pipettor 4100 and suck 280 μl of eluent;

[0093] S700. Rotate the rotary pipettor 4100 so that the offset tube is aligned with hole No. 1, lower the rotary pipettor 4100 to drain the liquid, blow and mix 20 times, and then raise it after completion;

[0094] S800. Place a magnet at the bottom of the magnetic bead lysis solution chamber to magnetize the magnetic beads, and let the magnetic beads adhere to the wall;

[0095] S900. Add samples to 5 PCR tubes in sequence;

[0096] Taking the sample addition to hole No. 5 as an example, it includes the following sub - steps:

[0097] S901. Rotate the rotary pipettor so that the offset tube is aligned with hole No. 1. Lower the rotary pipettor 4100 to aspirate 50 μl of liquid. After completion, raise the rotary pipettor 4100.

[0098] S902. Rotate the rotary pipettor so that the offset tube is aligned with hole No. 5. Lower the rotary pipettor 4100 to discharge the liquid, blow and mix it 10 times. After completion, raise it.

[0099] S903. Rotate the rotary pipettor so that the offset tube is aligned with hole No. 3. Lower the rotary pipettor 4100 to aspirate and discharge 20 times (to clean the pipette tip). After completion, raise it.

[0100] S1000. Rotate the rotary pipettor so that the offset tube is aligned with hole No. 4. Lower it to aspirate 280 μl of paraffin. After completion, raise it. Rotate the rotary pipettor and align it with holes No. 5 - 9 respectively. Lower it to discharge 50 μl of paraffin. After completion, raise it.

[0101] S1100. Rotate the rotary pipettor so that the offset tube is aligned with hole No. 3 (the pipette tip washing tube). Lower the rotary pipettor so that the offset tube reaches the bottom, unhook the pump interface, and keep the consumables tightly pressed.

[0102] S1200. Start the PCR reaction.

[0103] The above - mentioned embodiments are the preferred embodiments of the present application, which are only used to facilitate the description of the present application and do not impose any form of limitation on the present application. Any person with ordinary knowledge in the technical field, without departing from the technical features of the present application, uses the technical content disclosed in the present application to make local changes or modified equivalent embodiments, and without departing from the technical feature content of the present application, still belongs to the scope of the technical features of the present application.

Claims

1. An integrated device for sample processing and detection, characterized in that, Comprising: System housing assembly, load-carrying circular chassis assembly, rotary pipettor assembly; Wherein, the upper surface of the housing body is provided with a sample addition port, a pneumatic balance port, and a pipettor cylinder, and its lower part is provided with an opening; Wherein, the load-carrying circular chassis assembly includes: a load-carrying circular chassis; on the load-carrying circular chassis are evenly placed: a waste liquid bin, a magnetic bead lysis solution bin, reagent tubes, PCR tubes, and washing liquid bins; the central distances of the waste liquid bin, the magnetic bead lysis solution bin, the reagent tubes, the PCR tubes, and the washing liquid bins from the center of the load-carrying circular chassis are r; In the middle of the load-carrying circular chassis, an insertion cylinder is further provided; Wherein, the rotary pipettor assembly includes: a rotary pipettor; the rotary pipettor includes: a first vertical through pipe, a second vertical pipe, an offset pipette, and a bottom limiter; the offset pipette includes: an upper through pipe interface, a lower pipe interface, and an offset pipe; the lower part of the first vertical through pipe is connected to the upper through pipe interface, and the upper part of the second vertical pipe is connected to the lower pipe interface; the first vertical through pipe and the second vertical pipe are on the same vertical axis, and the first vertical through pipe is communicated with the offset pipe; the offset pipe is arranged parallel to the second vertical pipe and the axial distance between the two is also r; Wherein, the load-carrying circular chassis is hermetically connected to the lower opening of the housing body; Wherein, the top of the first vertical through pipe passes through the pipettor cylinder, the second vertical pipe and the bottom limiter are inserted into the insertion cylinder, and the outer diameter of the bottom limiter matches the inner diameter of the insertion cylinder.

2. The integrated device for sample processing and detection according to claim 1, wherein, The bottom limiter is a cylinder.

3. The integrated device for sample processing and detection according to claim 1, characterized in that, The sample addition port corresponds to the magnetic bead lysis solution bin.

4. An integrated device for sample processing and detection according to claim 1, wherein, The upper part of the first vertical through pipe has an air pump adapter, and the upper part of the first vertical through pipe is connected to an air pump.

5. An integrated device for sample processing and detection according to claim 4, characterized in that, A silica gel gasket is further provided between the first vertical through pipe and the air pump adapter.

6. The integrated device for sample processing and detection according to claim 1, wherein An anti-aerosol filter element is provided in the upper through pipe interface.

7. An integrated device for sample processing and detection according to claim 1, wherein, The number of magnetic bead lysis solution bins is 1, and lysis solution and magnetic beads are pre-stored in the magnetic bead lysis solution bin.

8. The integrated device for sample processing and detection according to claim 1, wherein, The number of waste liquid bins is 1.

9. The integrated device for sample processing and detection according to claim 1, wherein The number of washing liquid bins is 3, and washing liquid is pre-stored in all of them.

10. The integrated device for sample processing and detection according to claim 1, wherein The number of reagent tubes is 3, and elution liquid, elution liquid, and paraffin liquid are pre-stored respectively.

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