Anti-blocking tube reagent sampling device of pathological tissue independent drop dyeing system

Abstract

The utility model relates to the technical field of medical instruments, in particular to an anti-blocking tube reagent sampling device of a pathological tissue independent drop dyeing system. Comprising a reagent buffer tank, a peristaltic pump, a buffer tank mounting plate, a reagent box cover plate, a reagent bottle upper pressing plate, an RFID mounting plate, an RFID reader, a travel switch piece, a liquid level sensing piece, a reagent bottle piercing group, a reagent bottle, a reagent box right partition plate and a reagent box left partition plate, reagent bottles are inserted between the kit right partition plate and the kit left partition plate, and the reagent bottle piercing group pierces the reagent bottles; the reagent bottle piercing group at the high position sucks air, and the reagent bottle piercing group at the low position flows out reagent liquid and enters the reagent buffer tank until the reagent buffer tank is full of the reagent liquid; the peristaltic pump operates to extract reagent liquid in the reagent buffer tank, and the reagent liquid drips onto the glass slide through the pipeline, so that sampling of the reagent is realized, on-off of the pipeline does not need to be controlled through an electromagnetic valve, blockage at the electromagnetic valve is avoided, many later maintenance problems can be avoided, and the maintenance cost is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of medical devices, in particular to a reagent sampling device for preventing pipe blockage in a pathological tissue single-droplet staining system. Background Technique

[0002] Single-droplet HE staining is a commonly used staining method for staining tissue sections in histological research to observe and analyze the structure of tissues and the morphology of tissue cells. Single-droplet HE staining means placing a tissue section specimen in a staining trough frame and going through a series of processes such as heating and baking the slide, dewaxing, staining, cleaning, and mounting the slide to ensure the uniformity and accuracy of staining. The single-droplet HE staining machine is easy to operate, has a high-definition staining effect, and shortens the staining time. It is widely used in medical biology and pathological diagnosis. It can adapt to tissue sections of different sizes and shapes for staining treatment, reduce manual operation, and provide more accurate histological information.

[0003] At present, during the use of the reagents in the HE staining scheme on the market, due to the need to control the on-off of the pipeline through a solenoid valve, there is a phenomenon of pipeline blockage at the solenoid valve. After the pipeline is blocked, it is necessary to replace the pipeline and the solenoid valve in time or clean the pipeline in time, resulting in high maintenance costs. Content of the Utility Model

[0004] The purpose of the utility model is to provide a reagent sampling device for preventing pipe blockage in a pathological tissue single-droplet staining system, aiming to solve the problems of pipeline blockage of the solenoid valve and high maintenance costs in the traditional HE staining scheme.

[0005] To achieve the above object, in a first aspect, the present utility model provides a reagent sampling device for preventing pipe blockage in a pathological tissue single-drop staining system, including a reagent buffer tank assembly and a reagent chamber assembly. The reagent buffer tank assembly includes a reagent buffer tank, a peristaltic pump, and a buffer tank mounting plate. The reagent buffer tank is fixedly connected to the buffer tank mounting plate and is located on one side of the buffer tank mounting plate. The peristaltic pump is fixedly connected to the buffer tank mounting plate and is located on the top of the reagent buffer tank. The reagent chamber assembly includes a reagent kit cover plate, a reagent bottle upper pressing plate, an RFID mounting plate, an RFID reader, a travel switch member, a liquid level sensing member, a reagent bottle piercing group, a reagent bottle, a reagent kit right partition plate, and a reagent kit left partition plate. The reagent bottle upper pressing plate, the RFID mounting plate, the travel switch member, and the liquid level sensing member are respectively fixedly connected to the reagent kit cover plate and are all located outside the reagent kit cover plate. The RFID reader is fixedly connected to the RFID mounting plate and is located on one side of the RFID mounting plate. The reagent bottle piercing group is arranged on one side of the travel switch member. The reagent bottle is arranged on the side of the reagent kit cover plate away from the reagent bottle upper pressing plate. The reagent kit right partition plate is fixedly connected to the reagent kit cover plate and is located on the side of the reagent kit cover plate close to the reagent bottle. The reagent kit left partition plate is fixedly connected to the reagent kit cover plate and is located on the side of the reagent bottle away from the reagent kit right partition plate.

[0006] In an embodiment of the present utility model, the travel switch member includes a travel switch mounting plate and a travel switch. The travel switch mounting plate is fixedly connected to the reagent kit cover plate and is located outside the reagent kit cover plate. The travel switch is arranged on one side of the travel switch mounting plate.

[0007] In an embodiment of the present utility model, the liquid level sensing member includes a liquid level sensor mounting plate and a liquid level sensor. The liquid level sensor mounting plate is fixedly connected to the reagent kit cover plate and is located outside the reagent kit cover plate. The liquid level sensor is arranged on one side of the liquid level sensor mounting plate.

[0008] In an embodiment of the present utility model, the reagent bottle piercing group includes a reagent bottle puncture needle, a puncture needle mounting plate, a puncture needle baffle, and a male Luer connector. The puncture needle mounting plate is fixedly connected to the travel switch mounting plate and is located on one side of the travel switch mounting plate. The puncture needle baffle is fixedly connected to the puncture needle mounting plate and is located on one side of the puncture needle mounting plate. The male Luer connector is arranged on the side of the puncture needle baffle away from the puncture needle mounting plate. The reagent bottle puncture needle is arranged on the side of the puncture needle mounting plate away from the puncture needle baffle.

[0009] The anti-clogging tube reagent sampling device of the pathological tissue single-drop staining system of the present utility model. The buffer tank mounting plate provides mounting conditions for the reagent buffer tank and the peristaltic pump. The reagent buffer tank is used to store reagents. The peristaltic pump is used to extract the reagents in the reagent buffer tank and drip them onto the glass slide through a pipeline to achieve reagent sampling. The reagent bottle is used to store reagents. The reagent bottle puncturing group is used to puncture the reagent bottle and introduce the reagents in the reagent bottle into the reagent buffer tank for storage. The liquid level sensing member is used to sense the reagent liquid level in the reagent bottle. The reagent kit cover plate is fixed on the frame. The reagent bottle upper pressing plate is fixed on the top of the reagent kit cover plate and is used to limit the height direction of the reagent bottle. The left partition plate of the reagent kit and the right partition plate of the reagent kit are both fixed inside the reagent kit cover plate and are respectively used to limit the left and right bottle walls of the reagent bottle. The above-mentioned reagent bottle upper pressing plate, the right partition plate of the reagent kit and the left partition plate of the reagent kit together realize the limiting and fixing of one reagent bottle. Their materials are all spring steel and have good elastic deformation ability. The RFID mounting plate is fixed on the rear side of the top of the reagent kit cover plate. The RFID reader is fixed on the RFID mounting plate. Each reagent bottle has a unique corresponding ID code. The RFID reader is used to read the ID code on the reagent bottle. The travel switch mounting plate is fixed on the upper rear part of the reagent kit cover plate and is used to mount the travel switch and the reagent bottle puncturing group. The device inserts the reagent bottle between the right partition plate and the left partition plate of the reagent kit. The reagent bottle puncturing group punctures two silica gel plug gaskets at the high and low positions on the back of the reagent bottle. The reagent bottle puncturing group at the high position inhales air, and the reagent bottle puncturing group at the low position flows out the reagent liquid. The reagent liquid enters the reagent buffer tank until the reagent buffer tank is full. The peristaltic pump operates to extract the reagent liquid in the reagent buffer tank and drip the extracted reagent liquid onto the glass slide through a pipeline to achieve reagent sampling. It does not need to control the on-off of the pipeline through a solenoid valve, so there will be no blockage at the solenoid valve. The non-clogging of reagent sampling can avoid many later maintenance problems, reduce the maintenance cost of the instrument liquid path system, and solve the problems of solenoid valve pipeline blockage and high maintenance cost in the traditional HE staining scheme. Description of the Drawings

[0010] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0011] Figure 1It is a schematic structural diagram of the anti-clogging tube reagent sampling device of the pathological tissue single-drop staining system provided by the present utility model.

[0012] Figure 2 It is a schematic diagram of the reagent buffer tank assembly.

[0013] Figure 3 It is a schematic diagram of the reagent bin assembly.

[0014] Figure 4 It is a schematic diagram of the reagent bin assembly in another direction.

[0015] Figure 5 It is a schematic diagram of the reagent bottle piercing group.

[0016] Figure 6 It is a schematic diagram of the anti-clogging tube reagent sampling liquid path connection.

[0017] Figure 7 It is a schematic diagram of the anti-clogging tube reagent sampling liquid path principle.

[0018] Figure 8 It is a flowchart of the anti-clogging tube reagent sampling method of the pathological tissue single-drop staining system provided by the present utility model.

[0019] In the figure: 1 - reagent buffer tank assembly, 2 - reagent bin assembly, 100 - connecting pipe, 101 - reagent buffer tank, 102 - peristaltic pump, 103 - buffer tank mounting plate, 201 - reagent kit cover plate, 202 - reagent bottle upper pressing plate, 203 - RFID mounting plate, 204 - RFID reader, 205 - travel switch mounting plate, 206 - travel switch, 207 - liquid level sensor mounting plate, 208 - liquid level sensor, 209 - reagent bottle piercing group, 210 - reagent bottle, 211 - right partition plate of the reagent kit, 212 - left partition plate of the reagent kit, 301 - reagent bottle puncture needle, 302 - puncture needle mounting plate, 303 - puncture needle baffle, 304 - male Luer connector. Detailed implementation manners

[0020] The following details the embodiments of the present utility model. The examples of the embodiments are shown in the drawings. In the embodiments of the present utility model, the same or similar reference numerals represent the same or similar elements or elements with the same or similar functions from beginning to end. The embodiments described below with reference to the drawings are exemplary and are intended to explain the present utility model and should not be construed as limiting the present utility model.

[0021] Please refer to Figures 1 to 7, in a first aspect, the present utility model provides a reagent sampling device for preventing pipe blockage in a pathological tissue single-drop staining system, which includes a reagent buffer tank assembly 1 and a reagent chamber assembly 2. The reagent buffer tank assembly 1 includes a reagent buffer tank 101, a peristaltic pump 102, and a buffer tank mounting plate 103; the reagent buffer tank 101 is fixedly connected to the buffer tank mounting plate 103 and is located on one side of the buffer tank mounting plate 103, the peristaltic pump 102 is fixedly connected to the buffer tank mounting plate 103 and is located on the top of the reagent buffer tank 101; the reagent chamber assembly 2 includes a reagent kit cover plate 201, a reagent bottle upper pressing plate 202, an RFID mounting plate 203, an RFID reader 204, a travel switch 206, a liquid level sensing member, a reagent bottle piercing group 209, a reagent bottle 210, a reagent kit right partition plate 211, and a reagent kit left partition plate 212; the reagent bottle upper pressing plate 202, the RFID mounting plate 203, the travel switch 206, and the liquid level sensing member are respectively fixedly connected to the reagent kit cover plate 201 and are all located outside the reagent kit cover plate 201, the RFID reader 204 is fixedly connected to the RFID mounting plate 203 and is located on one side of the RFID mounting plate 203, the reagent bottle piercing group 209 is arranged on one side of the travel switch 206, the reagent bottle 210 is arranged on the side of the reagent kit cover plate 201 away from the reagent bottle upper pressing plate 202, the reagent kit right partition plate 211 is fixedly connected to the reagent kit cover plate 201 and is located on the side of the reagent kit cover plate 201 close to the reagent bottle 210, and the reagent kit left partition plate 212 is fixedly connected to the reagent kit cover plate 201 and is located on the side of the reagent bottle 210 away from the reagent kit right partition plate 211.

[0022] In this embodiment, the buffer tank mounting plate 103 provides an installation condition for the reagent buffer tank 101 and the peristaltic pump 102. The reagent buffer tank 101 is used to store reagents, the peristaltic pump 102 is used to extract the reagents in the reagent buffer tank 101 and drip them onto the glass slide through a pipeline to achieve reagent sampling. The reagent bottle 210 is used to store reagents, the reagent bottle piercing group 209 is used to pierce the reagent bottle 210 and introduce the reagents in the reagent bottle 210 into the reagent buffer tank 101 for storage, and the liquid level sensing member is used to sense the liquid level of the reagents in the reagent bottle 210.

[0023] The cover plate 201 of the reagent kit is fixed on the frame. The upper pressing plate 202 of the reagent bottle is fixed on the top of the cover plate 201 of the reagent kit and is used to limit the height of the reagent bottle 210. The left partition plate 212 and the right partition plate 211 of the reagent kit are both fixed inside the cover plate 201 of the reagent kit and are respectively used to limit the left and right bottle walls of the reagent bottle 210. The above-mentioned upper pressing plate 202 of the reagent bottle, the right partition plate 211 and the left partition plate 212 of the reagent kit together realize the limiting and fixing of one reagent bottle 210. Their materials are all spring steel and have good elastic deformation ability. The RFID mounting plate 203 is fixed at the rear side of the top of the cover plate 201 of the reagent kit. The RFID reader 204 is fixed on the RFID mounting plate 203. Each reagent bottle 210 has a unique corresponding ID code, and the RFID reader 204 is used to read the ID code on the reagent bottle 210. The travel switch mounting plate 205 is fixed at the upper rear part of the cover plate 201 of the reagent kit and is used to mount the travel switch 206 and the reagent bottle piercing group 209. By inserting the reagent bottle 210 between the right partition plate 211 and the left partition plate 212 of the reagent kit, the reagent bottle piercing group 209 pierces two silica gel plug stoppers at the high and low positions on the back of the reagent bottle 210. The reagent bottle piercing group 209 at the high position inhales air, and the reagent bottle piercing group 209 at the low position discharges reagent liquid. The reagent liquid enters the reagent buffer tank 101 until the reagent buffer tank 101 is full. The peristaltic pump 102 operates to extract the reagent liquid in the reagent buffer tank 101 and drips the extracted reagent liquid onto the glass slide through a pipeline, realizing the sampling of the reagent. There is no need to control the on-off of the pipeline through a solenoid valve, so there will be no blockage at the solenoid valve. The non-blocking of reagent sampling can avoid many subsequent maintenance problems, reduce the maintenance cost of the instrument liquid path system, and solve the problems of solenoid valve pipeline blockage and high maintenance cost in the traditional HE staining scheme.

[0024] Further, the travel switch 206 includes a travel switch mounting plate 205 and a travel switch 206. The travel switch mounting plate 205 is fixedly connected to the cover plate 201 of the reagent kit and is located outside the cover plate 201 of the reagent kit. The travel switch 206 is arranged on one side of the travel switch mounting plate 205.

[0025] In this embodiment, the travel switch mounting plate 205 is fixed on the cover plate 201 of the reagent kit to provide an installation condition for the travel switch 206. The travel switch 206 is used to be fixedly installed on the travel switch mounting plate 205 and is used for detecting whether the reagent bottle 210 is inserted in place.

[0026] Further, the liquid level sensing member includes a liquid level sensor mounting plate 207 and a liquid level sensor 208. The liquid level sensor mounting plate 207 is fixedly connected to the kit cover plate 201 and is located outside the kit cover plate 201. The liquid level sensor 208 is disposed on one side of the liquid level sensor mounting plate 207.

[0027] In this embodiment, the liquid level sensor mounting plate 207 provides an installation condition for the liquid level sensor 208. The liquid level sensor 208 is provided for monitoring the reagent in the reagent bottle 210. When the reagent in the reagent bottle 210 is about to be used up, an alarm signal is given through algorithm processing to prompt that a new reagent needs to be added.

[0028] Further, the reagent bottle piercing group 209 includes a reagent bottle puncture needle 301, a puncture needle mounting plate 302, a puncture needle baffle 303, and a male Luer connector 304. The puncture needle mounting plate 302 is fixedly connected to the travel switch mounting plate 205 and is located on one side of the travel switch mounting plate 205. The puncture needle baffle 303 is fixedly connected to the puncture needle mounting plate 302 and is located on one side of the puncture needle mounting plate 302. The male Luer connector 304 is disposed on the side of the puncture needle baffle 303 away from the puncture needle mounting plate 302. The reagent bottle puncture needle 301 is disposed on the side of the puncture needle mounting plate 302 away from the puncture needle baffle 303.

[0029] In this embodiment, the puncture needle mounting plate 302 provides an installation condition for the reagent bottle puncture needle 301. The reagent bottle puncture needle 301 is used to puncture the reagent bottle 210 to introduce air or extract the reagent. The puncture needle baffle 303 is used for limiting the reagent bottle puncture needle 301 to prevent the reagent bottle puncture needle 302 from being extruded during the insertion of the reagent bottle 210, ensuring that the reagent bottle puncture needle 302 can pierce the rubber stopper on the reagent bottle 210. The male Luer connector 304 is tightened on the reagent bottle puncture needle 302 for connecting the pipeline.

[0030] Please refer to Figure 8 , on the second aspect, the present invention also provides a method for sampling anti-blocking tube reagent of a pathological tissue single-drop staining system, which is applied to the anti-blocking tube reagent sampling device of the pathological tissue single-drop staining system as described in the first aspect above, and is characterized by including the following steps:

[0031] S1 Insert the reagent bottle 210 between the right partition plate 211 and the left partition plate 212 of the kit, and the reagent bottle piercing group 209 pierces the two silicone buckle plugs at the high and low positions on the back of the reagent bottle 210;

[0032] The reagent bottle piercing group 209 at the high position inhales air, and the reagent bottle piercing group 209 at the low position discharges reagent liquid and enters the reagent buffer tank 101 until the reagent buffer tank 101 is full.

[0033] In the embodiment of the present invention, the reagent bottle piercing group 209 at the high position realizes the inhalation of air and is connected with a one-way valve, which can only inhale or press in gas unidirectionally; the reagent bottle piercing group 209 at the low position realizes the outflow of reagent liquid and is connected with a one-way valve, which can only flow out reagent liquid unidirectionally. The liquid enters the reagent buffer tank 101, and the gas in the reagent buffer tank 101 flows out through the outlet at the upper part of the reagent buffer tank 101. After the gas in the reagent buffer tank 101 is exhausted, the reagent liquid also successfully fills the reagent buffer tank 101.

[0034] S3 The peristaltic pump 102 operates to extract the reagent liquid in the reagent buffer tank 101, and the extracted reagent liquid is dripped onto the glass slide through the pipeline to realize the sampling of the reagent.

[0035] In the embodiment of the present invention, there is a reagent outlet at the lower part of the reagent buffer tank 101, which is connected with a connecting pipe 100. The connecting pipe 100 is then placed into the peristaltic pump 102. The peristaltic pump 102 can work to extract the reagent liquid in the reagent buffer tank 101, and the extracted reagent liquid is dripped onto the glass slide through the pipeline to realize the sampling function of the reagent.

[0036] The above-disclosed is only the preferred embodiment of the anti-blocking tube reagent sampling device of the pathological tissue single-drop dyeing and staining system of the present invention. Of course, the scope of the rights of the present invention cannot be limited by this. Those of ordinary skill in the art can understand all or part of the processes of the above embodiments, and the equivalent changes made according to the claims of the present invention still fall within the scope covered by the present invention.

Claims

1. The anti-clogging reagent sampling device of the pathological tissue single-droplet staining system, characterized in that it includes a reagent buffer tank assembly and a reagent chamber assembly. The reagent buffer tank assembly includes a reagent buffer tank, a peristaltic pump, and a buffer tank mounting plate; the reagent buffer tank is fixedly connected to the buffer tank mounting plate and is located on one side of the buffer tank mounting plate. The peristaltic pump is fixedly connected to the buffer tank mounting plate and is located on top of the reagent buffer tank; the reagent chamber assembly includes a reagent kit cover plate, a reagent bottle upper pressing plate, an RFID mounting plate, an RFID reader, a travel switch component, a liquid level sensing component, a reagent bottle puncturing group, a reagent bottle, a reagent kit right partition plate, and a reagent kit left partition plate; the reagent bottle upper pressing plate, the RFID mounting plate, the travel switch component, and the liquid level sensing component are respectively fixedly connected to the reagent kit cover plate and are all located outside the reagent kit cover plate. The RFID reader is fixedly connected to the RFID mounting plate and is located on one side of the RFID mounting plate. The reagent bottle puncturing group is arranged on one side of the travel switch component. The reagent bottle is arranged on the side of the reagent kit cover plate away from the reagent bottle upper pressing plate. The reagent kit right partition plate is fixedly connected to the reagent kit cover plate and is located on the side of the reagent kit cover plate close to the reagent bottle. The reagent kit left partition plate is fixedly connected to the reagent kit cover plate and is located on the side of the reagent bottle away from the reagent kit right partition plate.

2. The anti-clogging reagent sampling device of the pathological tissue single-droplet staining system according to claim 1, characterized in that the travel switch component includes a travel switch mounting plate and a travel switch. The travel switch mounting plate is fixedly connected to the reagent kit cover plate and is located outside the reagent kit cover plate. The travel switch is arranged on one side of the travel switch mounting plate.

3. The anti-clogging reagent sampling device of the pathological tissue single-droplet staining system according to claim 1, characterized in that the liquid level sensing component includes a liquid level sensor mounting plate and a liquid level sensor. The liquid level sensor mounting plate is fixedly connected to the reagent kit cover plate and is located outside the reagent kit cover plate. The liquid level sensor is arranged on one side of the liquid level sensor mounting plate.

4. The anti-clogging reagent sampling device of the pathological tissue single-droplet staining system according to claim 2, characterized in that the reagent bottle puncturing group includes a reagent bottle puncturing needle, a puncturing needle mounting plate, a puncturing needle baffle, and a male Luer connector. The puncturing needle mounting plate is fixedly connected to the travel switch mounting plate and is located on one side of the travel switch mounting plate. The puncturing needle baffle is fixedly connected to the puncturing needle mounting plate and is located on one side of the puncturing needle mounting plate. The male Luer connector is arranged on the side of the puncturing needle baffle away from the puncturing needle mounting plate. The reagent bottle puncturing needle is arranged on the side of the puncturing needle mounting plate away from the puncturing needle baffle.

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