Counting plate based on microfluidics and liquid-based dyeing technology
By designing a counting board with microfluidic and liquid-based staining technology, the sample detection steps are integrated, and the problem of long and technical requirements of feces and urine inspection is solved, and automated, fast and accurate detection is achieved.
Patent Information
- Application Number
- CN202422261640.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-14
- Publication Date
- 2025-07-29
- Estimated Expiration
- 2034-09-14
AI Technical Summary
In the prior art, feces and urine examinations take a long time in the laboratory and have high technical requirements for operators. They are susceptible to human factors, resulting in poor production quality and inaccurate testing.
A counting plate based on microfluidic control and liquid-based dyeing technology is designed. By connecting the upper cover plate and the lower bottom plate to form sample filling holes, microflower cavity and discharge ports, integrating sample detection steps, simplifying the operation process, avoiding centrifugation, drying and dyeing, and automatically identifying and counting using AI intelligent picture recognition technology.
The detection steps are simplified, the detection efficiency is improved, the impact of human factors on the detection results is reduced, and the production quality and detection accuracy are ensured.
Smart Images
Figure CN223166555U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of formed component analysis, in particular to a counting plate based on microfluidics and liquid-based staining technology. Background Art
[0002] The formed components in urine include urine cells, casts, crystals, and microorganisms, etc. These components can all be used as indicators of the pet's health status. For example, urine cells can indicate urinary tract inflammation or infection, casts can reflect kidney problems such as renal tubular damage or glomerulonephritis, crystals can indicate urinary tract stones or metabolic disorders, and microorganisms indicate the possibility of infection, etc.
[0003] By observing the types and quantities of formed components in feces, veterinarians can understand the health status of the pet's digestive system: the appearance of red blood cells, white blood cells, and pus cells indicates the possibility of intestinal inflammation or infection; parasite eggs in feces indicate that the animal is infected with the corresponding parasites. Veterinarians can determine the types and infection degrees of parasites through further examinations and formulate corresponding deworming treatment plans; if a large amount of undigested food residues or fat droplets and other components are found in the feces, it indicates that the pet may have problems such as indigestion or improper diet. Veterinarians can help the pet improve the digestive condition by guiding the owner to adjust the pet's diet structure or change the food, etc.
[0004] The fecal examination in a conventional laboratory is a complex and time-consuming task, which requires multiple steps and operation processes. Methods such as smear method, flotation method, and fecal culture are all commonly used examination means. At the same time, for the examination of formed components in urine, a series of operation steps are also required: First, the urine needs to be centrifuged to remove the non-sediment components. Then, the sediment is made into a smear. Finally, the formed components are observed through a microscope. During the examination processes of feces and urine, specimen preparation not only takes a long time, but also has high technical requirements for the operator and is easily affected by human factors. Content of the Utility Model
[0005] The purpose of the utility model is to provide a counting plate based on microfluidics and liquid-based staining technology, which solves the technical problems that in the process of the existing laboratory examining feces and urine, specimen preparation not only takes a long time, but also has high technical requirements for the operator and is easily affected by human factors.
[0006] To achieve the above object, the present utility model provides a counting plate based on microfluidics and liquid-based staining technology, which includes a lower bottom plate and an upper cover plate. Guide edges are respectively arranged on the left and right sides of the front part of the lower bottom plate, and limit notches are respectively arranged on the left and right sides of the middle part. A concave microchannel cavity is arranged inside the lower bottom plate. The upper cover plate is connected to the lower bottom plate by a limiting rod. The upper cover plate is a flat plate structure. A sample addition hole and an overflow port are respectively arranged at the front end and the rear end of the upper cover plate. When the upper cover plate and the lower bottom plate are connected to form the counting plate, the sample addition hole is formed at the front part of the counting plate, the microchannel cavity is formed at the middle part, and the overflow port is formed at the rear part;
[0007] Viewed from directly above the counting plate, the microchannel cavity is composed of a trapezoid and a rectangle. The short side of the trapezoid is tangent to the sample addition hole or smaller than the sample addition hole. The long side of the trapezoid is equal to one side of the rectangle of the shooting observation window. The two waistlines of the trapezoid are the diversion edges of the microchannel cavity. The other side of the rectangle of the shooting observation window is tangent to the overflow port or smaller than the overflow port;
[0008] Viewed from the side cross-section of the counting plate, a blocking step is arranged at the connection between the overflow port and the microchannel cavity. The blocking step protrudes 50 - 400 μm on the lower bottom plate, making the cross-section smaller at this place.
[0009] Among them, the connection method between the upper cover plate and the lower bottom plate includes but is not limited to methods such as glue pasting, hot melting, laser welding or ultrasonic welding.
[0010] Among them, the structures of the sample addition hole and the overflow port include but are not limited to structures in the shape of a semi-circular or long strip waist shape.
[0011] Among them, the light transmittance of the shooting observation window area is ≥ 90%.
[0012] Among them, both the lower bottom plate and the upper cover plate use hydrophilic materials, or are surface-treated to cover nano hydrophilic polar groups, so that the water contact angle < 90°.
[0013] A counting plate based on microfluidics and liquid-based staining technology of the present utility model forms a counting plate by connecting the upper cover plate and the lower bottom plate, such that a sample adding hole is formed at the front part of the counting plate, a microchannel cavity is formed in the middle part, and an overflow port is formed at the rear part. Thus, a sample can enter from the sample adding hole and flow through the microchannel cavity, while the excess sample can flow out from the overflow port. Furthermore, each step of sample detection can be integrated in one counting plate, removing the complex liquid path structure and simplifying the detection steps. It not only eliminates the workload of specimen centrifugation, specimen spreading, specimen drying, specimen staining, and dried film, but also avoids the situation of inaccurate detection caused by poor preparation quality due to subjective factors such as the experience and level of operators. It solves the technical problems that in the process of examining feces and urine in existing laboratories, specimen preparation not only takes a long time, but also has high technical requirements for operators and is easily affected by human factors. Description of the Drawings
[0014] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art.
[0015] Figure 1 It is a schematic structural diagram of two composition situations of a counting plate based on microfluidics and liquid-based staining technology in the first embodiment of the present utility model.
[0016] Figure 2 It is a top view of two composition situations of a counting plate based on microfluidics and liquid-based staining technology in the first embodiment of the present utility model.
[0017] Figure 3 It is a schematic sectional view along the upper cover plate of a counting plate based on microfluidics and liquid-based staining technology in the first embodiment of the present utility model.
[0018] Figure 4 It is a schematic structural diagram of the lower bottom plate of a counting plate based on microfluidics and liquid-based staining technology in the first embodiment of the present utility model.
[0019] Figure 5 It is a schematic diagram of hydrophilic and hydrophobic properties in the first embodiment of the present utility model.
[0020] Figure 6 It is a schematic diagram of sample sampling in the first embodiment of the present utility model.
[0021] Figure 7 It is a schematic diagram of sample adding in the first embodiment of the present utility model.
[0022] Figure 8 It is a schematic diagram of the hatch popping out in the first embodiment of the present utility model.
[0023] Figure 9It is a schematic diagram of loading a microfluidic counting plate according to the first embodiment of the present utility model.
[0024] In the figure: 101 - lower bottom plate, 102 - upper cover plate, 103 - guiding edge, 104 - limiting notch, 105 - microchannel cavity, 106 - limiting rod, 107 - sample adding hole, 108 - overflow port, 109 - shooting and observing window, 110 - guiding edge, 111 - blocking step. Specific implementation manner
[0025] The embodiments of the present utility model will be described in detail below. The examples of the embodiments are shown in the drawings. The embodiments described below with reference to the drawings are exemplary and are intended to explain the present utility model and should not be construed as a limitation to the present utility model.
[0026] First embodiment:
[0027] Please refer to Figures 1 to 9 , the present utility model provides a counting plate based on microfluidics and liquid-based staining technology, including a lower bottom plate 101 and an upper cover plate 102.
[0028] In this embodiment, by connecting the upper cover plate 102 and the lower bottom plate 101 to form a counting plate, a sample adding hole 107 is formed at the front part of the counting plate, a microchannel cavity 105 is formed in the middle part, and an overflow port 108 is formed at the rear part. Thus, the sample can enter from the sample adding hole 107 and flow in the microchannel cavity 105, and the excess sample can flow out from the overflow port 108. Furthermore, each step of sample detection can be integrated in one counting plate, removing the complex liquid path structure and simplifying the detection steps. It not only eliminates the workload of specimen centrifugation, specimen smearing, specimen drying, specimen staining, and slide drying, but also avoids the situation of inaccurate detection caused by poor slide preparation quality due to subjective factors such as the experience and level of operators. It solves the technical problems that in the process of examining feces and urine in existing laboratories, specimen preparation not only takes a long time, but also has high technical requirements for operators and is easily affected by human factors.
[0029] Among them, guiding edges 103 are respectively arranged on the left and right sides of the front part of the lower bottom plate 101, limiting notches 104 are respectively arranged on the left and right sides of the middle part, and a concave microchannel cavity 105 is arranged inside the lower bottom plate 101. The upper cover plate 102 is connected to the lower bottom plate 101 in a limiting manner through a limiting rod 106. The upper cover plate 102 is of a flat plate structure. Sampling holes 107 and overflow ports 108 are respectively arranged at the front end and the rear end of the upper cover plate 102. When the upper cover plate 102 and the lower bottom plate 101 are connected to form a counting plate, the sampling hole 107 is formed in the front part of the counting plate, the microchannel cavity 105 is formed in the middle part, and the overflow port 108 is formed in the rear part. It should be noted that the shape of the limiting rod 106 includes but is not limited to circular, square, and hexagonal. In this solution, the limiting rods 106 are arranged in a circular diagonal layout, and the sizes of the cylinders are inconsistent to achieve an anti-fooling effect and prevent the cover glass from being installed in the wrong direction. At the same time, to ensure the imaging factor of the cells in the cavity due to the depth of field of the camera, the thickness of the upper cover plate 102 is set to 0.2 - 1 mm. In addition, the microchannel cavity 105 in the lower bottom plate 101 can also be realized in the upper cover plate 102, that is, the lower bottom plate 101 is a flat plate, and after the upper cover plate 102 is made with flow channel features, it is bonded and welded to the lower bottom plate 101. In addition, the limiting notch 104 can be structures such as steps, limiting rods 106, and protrusions that play the same limiting role.
[0030] Viewed from directly above the counting plate, the microchannel cavity 105 is composed of a trapezoid and a rectangle. The short side of the trapezoid is tangent to or smaller than the sampling hole 107, the long side of the trapezoid is equal to the long side of the rectangle of the shooting observation window 109, and the two waistlines of the trapezoid are the diversion edges 110 of the microchannel cavity 105, which can play a role in smooth and gradual transition of liquid drainage, prevent the liquid from not being able to effectively fill the cavity due to a sharp change in the channel size, resulting in voids or bubbles, and affect the image acquisition of the shooting observation window 109. At the same time, the other side of the shooting observation window 109 is tangent to or smaller than the overflow port, which helps the cavity to expel air bubbles and be filled with liquid in a short time.
[0031] Viewed from the side cross-section of the counting board, a blocking step 111 is provided at the connection between the overflow port 108 and the microchannel cavity 105. The blocking step 111 protrudes 50 - 400 μm on the lower bottom plate 101, reducing the cross-section at this location. When testing a blood specimen, the main height of the cross-section of the microchannel cavity 105 is 50 - 150 μm, and the height of the blocking step 111 is 20 - 100 μm; when testing a urine specimen, the main height of the cross-section of the microchannel cavity 105 is 100 - 300 μm, and the height of the blocking step 111 is 50 - 200 μm; when testing a fecal specimen, the main height of the cross-section of the microchannel cavity 105 is 150 - 500 μm, and the height of the blocking step 111 is 100 - 400 μm. The main function of the blocking step 111 is to prevent the formed components from flowing out of the effective detection area under the push of the liquid flow, thus causing a false negative detection result.
[0032] Secondly, the connection method between the upper cover plate 102 and the lower bottom plate 101 includes but is not limited to glue pasting, hot melting, laser welding, or ultrasonic welding.
[0033] Meanwhile, the structures of the sample addition hole 107 and the overflow port 108 include but are not limited to a semi-circular or long strip waist-shaped structure. The sample addition hole 107 and the overflow port 108 can also be elliptical holes, square holes, circular holes, etc. structures that play the same role. The end of the sample addition hole 107 adopts a symmetrical cicada head structure. After the left and right "cicada eyes" accommodate the sample, it spreads across the entire flow channel along the diversion line. When the sample volume is small, the sample stored in the "cicada eyes" can timely supplement the shortage of the flow channel sample to prevent vacancies in the flow channel.
[0034] In addition, the light transmittance of the shooting observation window 109 area is ≥90%.
[0035] Finally, both the lower bottom plate 101 and the upper cover plate 102 use hydrophilic materials, or are surface-treated to cover nano hydrophilic polar groups, making the water contact angle <90°. The hydrophilic materials include but are not limited to borosilicate glass, and the surface treatments include but are not limited to plasma treatment and nano coating.
[0036] When using a counting plate based on microfluidics and liquid-based staining technology of this embodiment, the upper cover plate 102 and the lower base plate 101 are connected by the limiting rod 106. After that, the upper cover plate 102 and the lower base plate 101 are bonded and welded together by gluing, hot welding, laser welding or ultrasonic welding, so that the upper cover plate 102 and the lower base plate 101 can together constitute a counting plate. At this time, the sample addition hole 107 is formed at the front of the counting plate, the microfluidic cavity 105 is formed in the middle, and the overflow port 108 is formed at the back, so that the sample can enter from the sample addition hole 107 and be deposited in the microchannel. The flow channel cavity 105 is circulated, and the excess sample can flow out from the overflow port 108, so that the various steps of sample detection can be integrated into a counting plate, eliminating the complex liquid path structure and simplifying the detection steps. It not only eliminates the workload of specimen centrifugation, specimen pushing, specimen drying, specimen staining, and slicing and drying, but also avoids the poor preparation quality caused by subjective factors such as operator experience and level, which leads to inaccurate detection. It solves the technical problem that in the process of feces and urine examination in existing laboratories, specimen preparation is not only time-consuming, but also has high technical requirements for the operator and is easily affected by human factors.
[0037] When testing animal samples, first obtain the animal samples. After collecting a sufficient amount of animal samples, add the animal samples to a container filled with dye solution, turn the animal samples upside down in the container to mix the samples, and after sufficient mixing, use a pipette to extract an appropriate amount of the stained mixed specimen. Then, place the counting plate horizontally on the table, use a pipette to add the mixed specimen to the sample addition hole 107 of the counting plate, and let it stand for a period of time. During the standing process, the sample will gradually fill the microfluidic cavity 105 under the action of siphoning, and the cells or formed elements in the sample will be in a suspended state at first. After standing for a period of time, the cells or formed elements in the sample will settle in the designated area of the bottom layer.
[0038] After sufficient sedimentation, first operate the instrument, pop out the hatch, and then align the guide edge 103 of the counting plate with the counting plate placement slot in the hatch according to the direction prompt of the counting plate. After alignment, push the counting plate inward. When the limit notches 104 on both sides of the counting plate are locked by the corresponding limit columns, operate the instrument, close the hatch, and let the instrument start testing. During the test, the instrument will take pictures of the cells and formed elements on the counting plate through a high-speed and high-magnification control camera, light source and mobile platform, and transmit the pictures taken to the information processing unit. Then, the information processing unit uses AI intelligent image recognition technology to automatically identify and count and classify various formed elements in the picture, and finally output the test results. At this time, open the hatch, take out the counting plate in the opposite direction, and complete the sample detection.
[0039] The above disclosure is only one or more preferred embodiments of the present application, and cannot be used to limit the scope of rights of the present application. Those of ordinary skill in the art can understand all or part of the processes of implementing the above embodiments, and the equivalent changes made according to the claims of the present application still fall within the scope covered by the present application.
Claims
1. A counting plate based on microfluidics and liquid-based staining technology, characterized in that it includes a lower bottom plate and an upper cover plate. On the left and right sides of the front part of the lower bottom plate, guiding edges are respectively provided. On the left and right sides of the middle part, limiting notches are respectively provided. And a concave microchannel cavity is arranged inside the lower bottom plate. The upper cover plate is connected to the lower bottom plate by a limiting rod. The upper cover plate is a flat plate structure. A sample adding hole and an overflow port are respectively arranged at the front end and the rear end of the upper cover plate. When the upper cover plate and the lower bottom plate are connected to form a counting plate, the sample adding hole is formed at the front part of the counting plate, the microchannel cavity is formed in the middle part, and the overflow port is formed at the rear part; Viewed from directly above the counting plate, the microchannel cavity is composed of a trapezoid and a rectangle. The short side of the trapezoid is tangent to or smaller than the sample adding hole. The long side of the trapezoid is equal to one side of the rectangle of the shooting observation window. The two waistlines of the trapezoid are the guiding edges of the microchannel cavity. The other side of the rectangle of the shooting observation window is tangent to or smaller than the overflow port; Viewed from the side cross-section of the counting plate, a blocking step is arranged at the connection between the overflow port and the microchannel cavity. The blocking step protrudes 50 - 400 μm on the lower bottom plate, making the cross-section smaller at this place.
2. The counting plate based on microfluidics and liquid-based staining technology according to claim 1, characterized in that the connection method between the upper cover plate and the lower bottom plate includes, but is not limited to, methods such as glue pasting, hot melting connection, laser welding or ultrasonic welding.
3. The counting plate based on microfluidics and liquid-based staining technology according to claim 1, characterized in that the structures of the sample adding hole and the overflow port include, but are not limited to, structures in the shape of a semi-circular or long strip waist shape.
4. The counting plate based on microfluidics and liquid-based staining technology according to claim 1, characterized in that the light transmittance of the shooting observation window area is ≥ 90%.
5. The counting plate based on microfluidics and liquid-based staining technology according to claim 1, characterized in that both the lower bottom plate and the upper cover plate are made of hydrophilic materials, or their surfaces are treated to cover nano hydrophilic polar groups, so that the water contact angle < 90°.