Simple magnetic solid-phase microextraction device for laboratory

Abstract

The utility model belongs to the technical field of magnetic solid-phase microextraction, and relates to a simple magnetic solid-phase microextraction device for a laboratory. The device comprises a magnetic separation frame capable of processing samples in batches and flexibly controlling an external magnetic field, and a separation tube capable of rapidly separating sample liquid. The magnetic separation frame comprises a separation frame main body with an isosceles trapezoid longitudinal section, a handle, test tube grooves positioned on the front side and the rear side of the separation frame main body, a test tube positioning clamping groove, a test tube arc mask, an adjustable magnetic block clamping groove, a movable magnetic block, a magnetic block fixer, and a sample receiving groove comprising a first surface and a second surface of the sample receiving groove. The separation tube can quickly separate sample liquid and comprises a threaded tube cap, a separation tube body, a sealing head liquid guide tube, a through hole with an upper opening, a small liquid outlet and a handle. According to the device, the whole set of flow of magnetic solid phase extraction can be completed in one set of device, the operation is simple, tedious and repeated manual operation is reduced, batch sample treatment is met, and meanwhile, the defects of high cross contamination probability and poor controllability in the extraction process can be avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of magnetic solid phase microextraction, and particularly relates to a simple magnetic solid phase microextraction device for laboratories. Background Art

[0002] The literature records that solid phase extraction technology is a sample pretreatment technology based on solid adsorbents. Its principle is to achieve the separation and enrichment of target components through the adsorption-desorption equilibrium between the solid adsorbent and the target components. Currently, the commonly used solid phase extraction method in research practice is to make the liquid sample pass through the adsorbent, retain the measured substance therein, then select a solvent with appropriate strength to wash away the impurities, and then elute the measured substance with a small amount of solvent, so as to achieve the purpose of rapid separation, purification and concentration. Although traditional solid phase extraction can enrich target analytes well, the operation is relatively cumbersome. The extraction process requires multiple rinses, the extraction time is long, and the adsorption column is prone to blockage, with poor repeatability.

[0003] Magnetic solid phase extraction technology is a dispersive solid phase extraction technology using magnetic or magnetizable materials as the adsorbent matrix. Compared with the traditional column solid phase extraction technology, magnetic solid phase extraction technology can significantly simplify the sample pretreatment steps. It can achieve phase separation only by applying an external magnetic field. Therefore, the operation is simple, time-saving and fast, without cumbersome operations such as centrifugation and filtration, and can avoid the problems of time-consuming loading of the traditional solid phase extraction adsorbent and sample loading or blockage of the extraction column.

[0004] With the rapid development of magnetic adsorbent material preparation technology, magnetic solid phase extraction technology is more and more widely used in the fields of environment, biology and food safety. Nevertheless, in laboratories, non-automated means are generally used for magnetic adsorption in magnetic solid phase extraction. Manual operations are still required in steps such as magnet adsorption, sample liquid aspiration, eluent addition, and eluent aspiration. Usually, an experimenter can only process one sample at a time, which affects the experimental processing speed and reduces the processing efficiency. To improve the efficiency, there are various types of magnetic separators on the market, most of which are conventional magnetic separation racks with single holes or multiple holes. That is, after the magnetic particles gather on the side with high magnetic field intensity, the liquid can be carefully sucked out immediately to complete solid-liquid separation. However, the magnet position in this magnetic separation rack is fixedly installed and cannot be adjusted. In operation, in order to make the external magnetic field appear and disappear, the test tube filled with solvent often needs to be taken out of the magnetic separation rack many times, and then put in after processing, which increases the workload and brings many inconveniences to the experiment. In addition, experimenters often use pipettes or disposable syringes to perform pipetting operations in ordinary test tubes / centrifuge tubes, and this process is extremely likely to cause contamination of the sample solution and loss of magnetic materials. Therefore, it is very necessary to develop a magnetic solid phase extraction device that can process samples in batches, flexibly control the external magnetic field and quickly separate the sample liquid.

[0005] Based on the current situation of the prior art, the inventors of the present application intend to provide a simple magnetic solid-phase microextraction device for laboratories. The device of the present application will be able to solve the defects of the existing magnetic separation devices, such as cumbersome operation, high probability of cross-contamination, and poor controllability. Summary of the Invention

[0006] The purpose of the present utility model is to provide a simple magnetic solid-phase microextraction device for laboratories in view of the problems existing in the prior art. Specifically, it relates to a magnetic solid-phase extraction device that can batch process samples, flexibly control the applied magnetic field, and quickly separate the sample solution. The present utility model will be able to solve the defects of the existing magnetic separation devices, such as cumbersome operation, high probability of cross-contamination, and poor controllability.

[0007] To achieve the above purpose, the present utility model provides a simple magnetic solid-phase microextraction device for laboratories, which includes a magnetic separation rack that can batch process samples and flexibly control the applied magnetic field, and a separation tube that can quickly separate the sample solution.

[0008] In the present utility model, the magnetic separation rack that can batch process samples and flexibly control the applied magnetic field includes a separation rack main body, a handle, test tube slots, test tube positioning card slots, test tube arc masks, adjustable magnet card slots, movable magnets, magnet holders, a sample receiving groove (including a first surface and a second surface of the sample receiving groove).

[0009] The separation rack main body is solidly poured, and the material can be plastic. Its longitudinal section is an isosceles trapezoid; the handles are respectively placed on the left and right sides of the separation rack main body, and the height from the bottom is the same.

[0010] Furthermore, the test tube slots are respectively located on the front side and the rear side of the separation rack main body, and 5 separation tubes can be placed on each side; the single length of the test tube slot is 6 - 8 cm, the inner diameter is 1.5 - 2.5 cm, its top is flush with the top of the separation rack main body, and the bottom is about 5 - 10 cm away from the bottom of the separation rack main body; the test tube slot forms an angle of 60 - 70° with the bottom horizontal plane of the separation rack main body, and the test tube slots on the front and rear sides are mirror-symmetrical.

[0011] The test tube positioning card slots are provided inside each test tube slot, and their positions are approximately 0.5 - 1.5 cm away from the top of the test tube slot. The material of the card slots is plastic, and their shape is the same as that of the test tube slot, with an inner diameter of approximately 1 - 2 cm; the test tube arc masks are provided on the front surface of each test tube slot, with a length of 3 - 5 cm, and their positions are parallel to the test tube slots, that is, they also form an angle of 60 - 70° with the horizontal plane at the bottom of the separation rack body; the top of the test tube arc mask is approximately 1 - 3 cm away from the top of the separation rack body, and the bottom is flush with the bottom of the test tube slot; the adjustable magnet card slots are respectively provided on the inner sides of the front and back sides of the separation rack body, that is, on the rear surfaces of the double-sided test tube slots. In particular, the rear surface of each test tube slot is partially hollowed out, and the length of the hollowed-out part is 6 - 8 cm, and the width is approximately 1 - 3 cm, which is connected to the magnet card slots; each test tube slot has an opening at the bottom end, and the diameter of the opening is 1.5 - 2.5 cm.

[0012] Further, the total width of the adjustable magnet card slots is 4 - 8 cm, initially set at the center line position of the test tube slot, and can be slid up and down as needed to fully separate the magnetic materials; the magnet holders are placed at the left and right ends of the double-sided adjustable magnet card slots; the movable magnets are two, respectively placed in the double-sided magnet card slots, with a width of 2 - 5 cm and a thickness of 0.1 - 0.3 cm.

[0013] The sample receiving slots are respectively placed on the front and back sides at the bottom of the separation rack body. In particular, the sample receiving slots are detachable, and the sample receiving slots have two surfaces. The first surface is a waste liquid tank, and the total length of the waste liquid tank is 12 - 20 cm, the width is 2 - 4 cm, and the depth is 1 - 2 cm; the second surface is obtained by flipping the first surface by 180°, and the second surface has 5 cylindrical grooves for placing centrifuge tubes for collecting desorbed liquid; further, the depth of the grooves is 1 - 2 cm, and the surface diameter is 1 - 2 cm. Specifically, the position of each groove corresponds to the position of the opening at the bottom end of the above test tube slot.

[0014] In the present utility model, a separation tube capable of quickly separating sample liquid includes a threaded tube cap, a separation tube body, a sealing head, a liquid guide tube, a through hole (including the upper opening of the through hole), a small liquid outlet, and a handle.

[0015] The length of the separation tube body is 7 - 12 cm, and the inner diameter is 1 - 2 cm. It is a hollow cavity with an upper opening, and the threaded tube cap is placed at the upper end of the test tube body; the bottom end of the separation tube body is provided with a through hole, and there is a thread on the inner wall of the through hole. The through hole includes a lower opening and an upper opening; the sealing head is in the shape of an inverted cone or an inverted frustum of a cone, and the maximum outer diameter of the sealing head needs to be greater than the maximum inner diameter of the upper opening of the through hole.

[0016] Further, the sealing head is in tight fit with the upper opening of the through hole to form a seal, that is, the shape of the sealing head matches the shape of the upper opening of the through hole. The bottom radius of the sealing head is smaller than the inner diameter of the upper opening of the through hole. The bottom end of the sealing head is inserted into the upper opening of the through hole, and the part of the sealing head exposed above the upper opening of the through hole matches the bottom shape of the separation tube body. The side surface of the sealing head is in tight contact with the inner bottom surface of the separation tube body to form a seal.

[0017] Further, the bottom end of the sealing head is connected to the top end of the liquid guiding tube. The liquid guiding tube is a hollow cylindrical body with a length of about 2 - 5 cm. The outer wall of the part where the liquid guiding tube is connected to the sealing head and the upper edge is provided with threads, and the threads provided thereon are in threaded fit with the through hole at the bottom end of the separation tube body.

[0018] Further, two rows of small liquid outlets are respectively provided at the lower edge adjacent to the outer wall threads above, and the diameter of the small liquid outlets does not exceed 0.8 mm.

[0019] Further, outer wall threads are also provided at the lower edge adjacent to the small liquid outlets above. The threads provided thereon are used for threaded fit with the through hole at the bottom end of the separation tube body when separating liquids, so as to avoid liquid leakage.

[0020] More optimally, the handle is fixed on the outer wall of the liquid guiding tube. It is placed at the lower edge of the above-mentioned threads. The handle provided is used to tighten / loosen the threads, and the advantage is that it is labor-saving and convenient.

[0021] When the magnetic separation rack and the separation tube provided by the present utility model are in use, first, the liquid guiding tube below the separation tube is tightened counterclockwise, so that the sealing head tightly fits the upper opening of the through hole to form a seal. Then, the material to be tested, the extraction / desorption sample and the extraction / desorption solvent are added into the test tube body. After tightening the threaded tube cap, the separation tube is placed in the corresponding double-sided test tube slot, and then the entire magnetic separation rack is placed on an oscillator for dynamic adsorption / desorption. During the adsorption / desorption process, the inclined double-sided test tube slot design increases the contact area between the sample and the magnetic material, and the magnetic material is not easily settled at the bottom of the test tube due to the gravity effect. Therefore, the adsorption / desorption is more sufficient. After the adsorption / desorption is completed, two magnetic blocks are respectively inserted into the card slots on the back of the double-sided test tube slot, fixed after adjusting the height, and left standing for solid-liquid separation. When separating the liquid, the first surface (for collecting waste liquid) or the second surface (for collecting desorbed liquid) of the double-sided sample receiving groove is placed below the test tube slot. First, loosen the threaded tube cap, and then gently rotate the liquid guiding tube clockwise, so that the sealing head gradually rises above the surface of the upper opening of the through hole, and the liquid in the test tube body flows out through the small liquid outlets provided on the liquid guiding tube and enters the waste liquid groove or the sample groove to complete the liquid separation.

[0022] Compared with the prior art, the present invention has the following advantages:

[0023] (1) Compared with the existing magnetic solid-phase extraction device, the solution provided by the present utility model can complete the entire process of magnetic solid-phase extraction in a set of devices, reducing cumbersome and repetitive manual operations and meeting the requirements for batch sample processing.

[0024] (2) In the magnetic separation rack designed by the present utility model, the magnetic blocks are movable, and the magnetic field can appear or disappear according to experimental needs, avoiding the need to repeatedly take out the test tubes filled with solvents from the magnetic separation rack, process them, and then put them back, thus improving convenience and experimental efficiency.

[0025] (3) In the magnetic separation rack designed by the present utility model, the test tubes containing samples are inclined and fixed in the sample slots. Therefore, during the oscillation process, the contact area between the material and the solvent is larger, and the extraction is more sufficient; at the same time, during the liquid separation process, the inclined tube rack design also makes it easier for the liquid to flow out from the liquid guide tube.

[0026] (4) The separation test tubes designed by the present utility model drain liquid from the lower outlet, avoiding incomplete liquid transfer or material loss caused by using a pipette during liquid separation. The entire liquid separation process is simple to operate, enabling complete liquid separation and improving the experimental quality. Description of the Drawings

[0027] Figure 1 Are the three-view drawings of a simple magnetic solid-phase microextraction device for laboratory use.

[0028] Figure 2 Is the overall structure diagram of a simple magnetic solid-phase microextraction device for laboratory use.

[0029] Figure 3 Is the structure diagram of a magnetic separation rack that can batch process samples and flexibly control the external magnetic field.

[0030] Figure 4 Is the structure diagram of a separation tube that can quickly separate the sample liquid.

[0031] Figure 5 Is the operation flow chart of a simple magnetic solid-phase microextraction device for laboratory use. Detailed Implementation Manner

[0032] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0033] Such as Figure 2As shown, a simple magnetic solid-phase microextraction device for laboratories includes a magnetic separation rack capable of batch processing samples and flexibly controlling an externally applied magnetic field, and a separation tube capable of quickly separating sample liquids.

[0034] Specifically, as Figure 2 and 3 shown, a magnetic separation rack capable of batch processing samples and flexibly controlling an externally applied magnetic field includes a separation rack main body 1, a handle 2, a test tube slot 3, a test tube positioning card slot 4, a test tube arc mask 5, an adjustable magnet card slot 6, a movable magnet 7, a magnet holder 8, and a sample receiving groove 9 (including a first surface 10 and a second surface 11 of the sample receiving groove).

[0035] More preferably, the separation rack main body 1 is solidly filled, and the material can be plastic. Its longitudinal section is an isosceles trapezoid; the handles 2 are respectively placed on the left and right sides of the separation rack main body 1, and the height from the bottom is the same (as Figure 1 shown).

[0036] Further, the test tube slots 3 are respectively located on the front side and the rear side of the separation rack main body 1, and 5 separation tubes can be placed on each side; the single length of the test tube slot 3 is 6 - 8 cm, the inner diameter is 1.5 - 2.5 cm, its top is flush with the top of the separation rack main body 1, and the bottom is about 5 - 10 cm away from the bottom of the separation rack main body 1; the test tube slot 3 forms an angle of 60 - 70° with the horizontal plane of the bottom of the separation rack main body 1, and the test tube slots 3 on the front and rear sides are mirror-symmetrical.

[0037] The test tube positioning card slot 4 is arranged inside each test tube slot 3, and its position is about 0.5 - 1.5 cm away from the top of the test tube slot 3. The material of the card slot is plastic, and its shape is the same as that of the test tube slot 3, and the inner diameter is about 1 - 2 cm; the test tube arc mask 5 is arranged on the front surface of each test tube slot 3, with a length of 3 - 5 cm, and its position is parallel to the test tube slot 3, that is, it also forms an angle of 60 - 70° with the horizontal plane of the bottom of the separation rack main body 1; the top of the test tube arc mask 5 is about 1 - 3 cm away from the top of the separation rack main body 1, and the bottom is flush with the bottom of the test tube slot 3; the adjustable magnet card slots 6 are respectively arranged on the inner sides of the front and rear sides of the separation rack main body 1, that is, on the rear surfaces of the bilateral test tube slots 3. Specifically, a part of the rear surface of each test tube slot 3 is hollowed out, the length of the hollowed-out part is 6 - 8 cm, the width is about 1 - 3 cm, and it is communicated with the magnet card slot 6; each test tube slot 3 has a hole at the bottom end, and the hole diameter is 1.5 - 2.5 cm.

[0038] Further, the total width of the adjustable magnet card slot 6 is 4 - 8 cm, initially arranged at the center line position of the test tube slot 3, and can be slid up and down as needed to fully separate magnetic materials; the magnet holder 8 is placed at the left and right ends of the bilateral adjustable magnet card slots 6; the movable magnets 7 are two pieces, respectively placed in the bilateral magnet card slots 6, with a width of 2 - 5 cm and a thickness of 0.1 - 0.3 cm.

[0039] The sample receiving grooves 9 are respectively arranged on the front and rear sides of the bottom of the separation rack main body 1. Particularly, the sample receiving grooves 9 are detachable, and the sample receiving groove has two surfaces. The first surface is the waste liquid groove 10, and the total length of the waste liquid groove 10 is 12 - 20 cm, the width is 2 - 4 cm, and the depth is 1 - 2 cm; its second surface 11 is obtained by flipping the first surface by 180°. Five cylindrical grooves are provided on the second surface 11 for placing centrifuge tubes for collecting desorbed liquid; further, the depth of the groove is 1 - 2 cm, and the surface diameter is 1 - 2 cm. Specifically, the position of each groove corresponds to the position of the hole opened at the bottom end of the above test tube slot.

[0040] In addition, as Figure 2 and 4 shown, a separation tube capable of quickly separating sample liquid is characterized by comprising a threaded tube cap 12, a separation tube body 13, a sealing head 14, a liquid guiding tube 15, a through hole 16 (including the upper opening 19 of the through hole), a small liquid outlet 17, and a handle 18. [[ID=,10]]

[0041] The height of the separation tube body 13 is 7 - 12 cm, and the inner diameter is 1 - 2 cm. It is a hollow cavity with an upper opening, and the threaded tube cap 12 is placed at the upper end of the separation tube body 13; a through hole 16 is provided at the bottom end of the separation tube body 13, and there is a thread on the inner wall of the through hole 16. The through hole 16 includes an upper opening 19 and a lower opening; the sealing head 14 is in the shape of an inverted cone or an inverted frustum, and the maximum outer diameter of the sealing head 14 needs to be greater than the maximum inner diameter of the upper opening 22 of the through hole.

[0042] Further, the sealing head 14 and the upper opening 19 of the through hole are closely fitted to form a seal, that is, the shape of the sealing head 14 matches the shape of the upper opening 19 of the through hole. The bottom surface radius of the sealing head 14 is smaller than the inner diameter of the upper opening 19 of the through hole. The bottom end of the sealing head 14 is inserted into the upper opening 19 of the through hole. The part of the sealing head 14 exposed outside the upper opening 19 of the through hole matches the bottom surface shape of the separation tube body 13, and the side surface of the sealing head 14 is in close contact with the inner bottom surface of the separation tube body 13 to form a seal.

[0043] Further, the bottom end of the sealing head 14 is connected to the top end of the liquid guiding tube 15. The liquid guiding tube 15 is a hollow cylindrical body with a length of about 2 - 5 cm. Threads are provided on the outer wall of the part where the liquid guiding tube 15 is connected to the sealing head 14 and at the upper edge, and the threads provided thereon are in threaded fit with the threads of the through hole at the bottom end of the separation tube body 13

[0044] Further, two rows of small liquid outlets 17 are respectively provided along the lower edge adjacent to the above outer wall threads, and the diameter of the small liquid outlets 17 does not exceed 0.8 mm.

[0045] Further, an outer wall thread is also provided at the lower edge adjacent to the small liquid outlet. The thread is used for threaded cooperation with the through hole at the bottom end of the separation tube body when separating liquids, so as to avoid liquid leakage.

[0046] More optimally, the handle is fixed to the outer wall of the liquid guide tube and is located at the lower edge of the above-mentioned thread. The handle is used to tighten / loosen the thread, and the advantage is that it is labor-saving and convenient.

[0047] Embodiment

[0048] A simple magnetic solid-phase microextraction device for laboratories includes a magnetic separation rack that can batch-process samples and flexibly control an external magnetic field, and a separation tube that can quickly separate sample liquids. Among them, a magnetic separation rack that can batch-process samples and flexibly control an external magnetic field consists of a separation rack main body 1, a handle 2, a test tube slot 3, a test tube positioning card slot 4, a test tube arc mask 5, an adjustable magnet card slot 6, a movable magnet 7, a magnet holder 8, a sample receiving groove 9 (including a first surface 10 and a second surface 11) of the sample receiving groove. The separation tube that can quickly separate sample liquids is characterized in that it includes a threaded tube cap 12, a separation tube body 13, a sealing head 14, a liquid guide tube 15, a through hole 16 (including an upper opening 19 of the through hole), a small liquid outlet 17, and a handle 18.

[0049] More optimally, the single length of the test tube slot 3 is 7 cm, the inner diameter is 1.8 cm, and the bottom of it is about 6 cm away from the bottom of the separation rack main body 1; the position of the test tube positioning card slot 4 is about 2 cm away from the top of the test tube slot 3, and the inner diameter is about 1.6 cm; the length of the test tube arc mask 5 is 5.5 cm, and the top of it is about 1.5 cm away from the top of the separation rack main body 1; the length of the hollow part on the rear surface of each test tube slot 3 is 6.8 cm, and the width is about 1.5 cm; the diameter of the opening below each test tube slot 3 is 1.8 cm; the total width of the adjustable magnet card slot 6 is 5 cm, the width of the movable magnet is 3 cm, and the thickness is 0.1 cm. The total length of the first surface 10 of the sample receiving groove 9 is 15 cm, the width is 3 cm, and the depth is 1 cm. The depth of the groove provided on the second surface 11 is 1 cm, and the surface diameter is 2 cm.

[0050] Further, for the separation tube that can quickly separate sample liquids, the height of its tube body 13 is 8 cm, and the inner diameter is 1.5 cm; the length of the liquid guide tube 15 is about 3.5 cm; the part of the liquid guide tube 15 connected to the sealing head 14 and the outer wall at the upper edge 3 mm have threads. Two rows of small liquid outlets are respectively provided at 0.5 mm and 0.7 mm below the lower edge of the above-mentioned outer wall thread. An outer wall thread is provided adjacent to the small liquid outlet and 3 mm below its lower edge.

[0051] In this embodiment, the working principle of the present utility model is further illustrated through an actual magnetic solid-phase extraction experiment. The magnetic material synthesized in this laboratory is selected for the pretreatment of perfluorinated compounds in water quality. The specific steps are as follows( Figure 5 ):

[0052] (1) Loading the liquid: First, tighten the liquid guide tube below the separation tube counterclockwise so that the sealing head tightly fits the upper opening of the through hole to form a seal. Then, take 5 mg of magnetic material, 2.5 mL of water sample and 2.5 mL of methanol and add them into the separation tube body, and tighten the threaded tube cap.

[0053] (2) Adsorption: Place the separation tube filled with liquid and adsorption material in the corresponding double-sided test tube slot, and then place the entire magnetic separation rack on the oscillator for dynamic adsorption.

[0054] (3) Magnetic separation of solid and liquid phases: After the adsorption is completed, insert two magnetic blocks into the card slots on the back of the double-sided test tube slot respectively, adjust the height and then fix it, and let it stand to complete the solid-liquid separation.

[0055] (4) Separating the waste liquid: Place the first surface of the double-sided sample receiving groove under the test tube slot. First, loosen the threaded tube cap, and then gently turn the liquid guide tube clockwise so that the sealing head gradually rises above the upper opening surface of the through hole, and the liquid in the test tube body flows out through the small liquid outlet provided on the liquid guide tube and enters the waste liquid tank to complete the liquid separation.

[0056] (5) Desorption: Tighten the liquid guide tube below the separation tube again, and then add 5 mL of 0.l% ammoniated methanol desorption solution. Take out the magnetic blocks from the card slots on the back of the double-sided test tube slot, tighten the threaded tube cap again, and then place the entire magnetic separation rack in the ultrasonic instrument for desorption.

[0057] (6) Collecting the desorption solution: After completion, insert two magnetic blocks into the card slots on the back of the double-sided test tube slot respectively, adjust the height and then fix it, and let it stand to complete the solid-liquid separation. Further, place the second surface of the double-sided sample receiving groove under the test tube slot, and place the corresponding collection tubes in the 5 grooves, and then repeat the subsequent steps of step (3) to complete the collection of the desorption solution.

[0058] The experimental results show that the simple magnetic solid-phase microextraction device of the present utility model can complete the whole process of magnetic solid-phase extraction in a set of devices, with simple operation, reducing cumbersome and repetitive manual operations, meeting the requirements of batch sample processing, and at the same time avoiding the defects of high probability of cross-contamination and poor controllability in the extraction process.

[0059] The above embodiments are only explanations of the present invention, but the implementation manners of the present invention are not limited by the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principle of the present invention shall be equivalent replacement methods and are all included in the protection scope of the present invention.

Claims

1. A simple magnetic solid phase microextraction device for laboratory use, characterized in that: The invention comprises a magnetic separation rack capable of batch processing samples and flexibly controlling an external magnetic field, and a separation tube capable of rapidly separating sample liquids; The magnetic separation rack capable of batch processing samples and flexibly controlling an external magnetic field comprises a separation rack body, a handle, a test tube slot, a test tube positioning slot, a test tube arc mask, an adjustable magnetic block slot, a movable magnetic block, a magnetic block holder, and a sample receiving slot, wherein the sample receiving slot comprises a first surface and a second surface; The separation frame body is solid and cast, and its longitudinal section is an isosceles trapezoid; the handles are respectively placed on the left and right sides of the separation frame body and are at the same height from the bottom; The test tube slots are located on the front and back sides of the separation rack body, each capable of accommodating five separation tubes. Each test tube slot is 6-8 cm long and has an inner diameter of 1.5-2.5 cm. Its top is flush with the top of the separation rack body, and its bottom is approximately 5-10 cm from the bottom of the separation rack body. The test tube slots form a 60-70° angle with the horizontal plane of the bottom of the separation rack body, and the test tube slots on the front and back sides are mirror-image symmetrical. The separation tube capable of quickly separating sample liquid comprises a threaded tube cap, a separation tube body, a sealing head, a liquid guide tube, a through hole, a small liquid outlet and a handle, wherein the through hole comprises an upper opening of the through hole.

2. The simple magnetic solid phase microextraction device according to claim 1, characterized in that: The test tube positioning slot is provided inside each test tube slot, and is located approximately 0.5-1.5 cm from the top of the test tube slot. The slot is made of plastic, and its shape is consistent with the shape of the test tube slot, with an inner diameter of approximately 1-2 cm. The test tube arc mask is provided on the front surface of each test tube slot, is 3-5 cm long, and is located parallel to the test tube slot, that is, it also forms an angle of 60-70° with the horizontal plane of the bottom of the separation rack body. The top of the test tube arc mask is approximately 1-3 cm away from the top of the separation rack body, and the bottom is flush with the bottom of the test tube slot. The adjustable magnetic block slots are respectively provided on the inner sides of the front and rear sides of the separation rack body, that is, on the rear surfaces of the test tube slots on both sides. The rear surface of each test tube slot is hollowed out, and the hollow part is 6-8 cm long and approximately 1-3 cm wide, which is connected to the magnetic block slot. The bottom end of each test tube slot has a hole with a hole diameter of 1.5-2.5 cm.

3. The simple magnetic solid phase microextraction device according to claim 1, characterized in that: The total width of the adjustable magnetic block slot is 4-8 cm. It is initially located at the center line of the test tube slot and can be slid up and down as needed to fully separate the magnetic material. The magnetic block holder is placed at the left and right ends of the double-sided adjustable magnetic block slot. There are two movable magnetic blocks, which are placed in the double-sided magnetic block slots respectively. Their width is 2-5 cm and their thickness is 0.1-0.3 cm.

4. The simple magnetic solid phase microextraction device according to claim 1, characterized in that: The sample receiving slots are respectively placed The sample receiving slots on the front and back sides of the bottom of the separation rack body are detachable and have two surfaces, the first surface of which is a waste liquid slot with a total length of 12-20 cm, a width of 2-4 cm, and a depth of 1-2 cm; the second surface is obtained by flipping the first surface 180°, and the second surface is provided with 5 cylindrical grooves for placing centrifuge tubes for collecting desorption liquid; the grooves are 1-2 cm deep and 1-2 cm in diameter, and the position of each groove corresponds to the position of the hole at the bottom of the above-mentioned test tube slot.

5. The simple magnetic solid phase microextraction device according to claim 1, characterized in that: The separation tube body is 7-12 cm long and has an inner diameter of 1-2 cm. It is a hollow cavity with an upper opening, and the threaded cap is placed on the upper end of the test tube body. The bottom end of the separation tube body is provided with a through hole with threads on the inner wall of the through hole. The through hole includes a lower opening and an upper opening. The sealing head is in the shape of an inverted cone or an inverted truncated cone, and the maximum outer diameter of the sealing head needs to be larger than the maximum inner diameter of the upper opening of the through hole.

6. The simple magnetic solid phase microextraction device according to claim 5, characterized in that: The sealing head is tightly matched with the upper opening of the through hole to form a seal, that is, the shape of the sealing head is matched with the shape of the upper opening of the through hole, the bottom radius of the sealing head is smaller than the inner diameter of the upper opening of the through hole, the bottom end of the sealing head is inserted into the upper opening of the through hole, the part of the sealing head exposed from the upper opening of the through hole is matched with the bottom shape of the separation tube body, and the side surface of the sealing head is in close contact with the inner bottom surface of the separation tube body to form a seal.

7. The simple magnetic solid phase microextraction device according to claim 5, characterized in that: The bottom end of the sealing head is connected to the top end of the liquid guide tube. The liquid guide tube is a hollow cylinder with a length of about 2-5 cm. The outer wall of the portion where the liquid guide tube is connected to the sealing head and the upper edge is provided with threads, which are matched with the threads of the through hole at the bottom end of the separation tube body; two rows of small liquid outlets are provided at the lower edge of the above-mentioned outer wall threads, and the diameter of the small liquid outlets does not exceed 0.8 mm.

8. The simple magnetic solid phase microextraction device according to claim 7, characterized in that: An outer wall thread is also provided at the lower edge of the small liquid outlet, and the thread is used to cooperate with the thread of the through hole at the bottom end of the separation tube body when separating the liquid to avoid leakage.

9. The simple magnetic solid phase microextraction device according to claim 1, characterized in that: The handle is fixed to the outer wall of the catheter and is placed at the lower edge of the thread. The handle is used to tighten / loosen the thread, which is labor-saving and convenient.

Images