Insoluble recombinant protein recovery device
By designing an insoluble recombinant protein resuscitation device that drives the stirring roller and scraper, the problem of insoluble recombinant protein solution accumulation in the inner wall of the tank body is solved, achieving uniform mixing and cleaning of the inner wall, reducing precipitation and promoting resuscitation effect.
Patent Information
- Application Number
- CN202422534958.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-21
- Publication Date
- 2025-08-15
- Estimated Expiration
- 2034-10-21
AI Technical Summary
In the prior art, insoluble recombinant protein solution accumulates on the inner wall of the tank, resulting in uneven mixing, affecting the resuscitation effect, and at the same time, the feed rate cannot be adjusted, resulting in precipitation.
A insoluble recombinant protein resuscitation device including a rotary shaft and agitating roller is designed. The solution in the tank is fully stirred by driving the stirrer roller and scraper through the rotary shaft, and the feed rate is adjusted through the valve to ensure uniform mixing of the solution and clean the inner wall.
The uniform mixing of insoluble recombinant protein solutions is achieved, which promotes resuscitation effect, and effectively cleans the inner wall of the tank body to reduce precipitation.
Smart Images
Figure CN223221337U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of recombinant protein recovery, in particular to an insoluble recombinant protein recovery device. Background Art
[0002] Biologically active proteins expressed by cells often exist in the form of fusible or molecular complexes, and functional proteins expressed are always folded into specific three-dimensional structures. When exogenous genes are expressed in prokaryotic cells, the recombinant proteins expressed are usually present in the form of insoluble proteins in inclusion bodies. Inclusion bodies are high-density, insoluble protein particles wrapped by membranes formed when highly expressed in Escherichia coli. The proteins in inclusion bodies are aggregates in an unfolded state and usually have no biological activity. Therefore, recombinant proteins need to be revived or renatured before they can be used.
[0003] However, the prior art still has the following problems:
[0004] First, the insoluble recombinant protein solution needs to ensure that the various components in the solution are evenly distributed in order to promote the recovery of the insoluble recombinant protein. However, in the existing technology, the substances attached to the inner wall of the tank will accumulate on the inner wall of the tank, making it inconvenient to mix the recombinant protein solution to promote recovery and inconvenient to clean the dirt on the inner wall of the tank, affecting the reaction effect.
[0005] Secondly, by adjusting the feed rate of the recombinant protein solution, the rate of change of the denaturant concentration in the dialysis tank can be changed to minimize precipitation. The existing feed rates on the market are all uniform and it is not easy to adjust the feed rate, and precipitation will occur during the recovery process of the recombinant protein.
[0006] In response to the above problems, the inventors proposed an insoluble recombinant protein resuscitation device to solve the above problems. Utility Model Content
[0007] In order to solve the problems of inconvenience in mixing the recombinant protein solution to promote recovery, cleaning the dirt on the inner wall of the tank and adjusting the feeding speed, the purpose of the utility model is to provide an insoluble recombinant protein recovery device.
[0008] In order to solve the above technical problems, the present invention adopts the following technical solutions: an insoluble recombinant protein recovery device, comprising a bottom plate, one side of the top of the bottom plate is fixedly connected to a conveying device, the other side of the top of the bottom plate is fixedly connected to a mixing device, the mixing device comprises a tank body, the bottom end of the tank body is fixedly connected to the top of the bottom plate, the upper and lower inner walls of the tank body are connected to a rotating shaft for common rotation, the top of the tank body is fixedly connected to a support plate, the upper inner wall of the support plate is fixedly connected to a motor, and the output end of the motor passes through the top of the tank body and is fixedly connected to the top of the rotating shaft, and the outer surface of the rotating shaft is fixedly connected to a plurality of first A stirring roller, a plurality of second stirring rollers are fixedly connected to the outer surface of the rotating shaft, a plurality of third stirring rollers are fixedly connected to the outer surface of the rotating shaft, the plurality of first stirring rollers, the second stirring rollers and the third stirring rollers are all distributed in a circular array, and the sizes of the plurality of first stirring rollers, the second stirring rollers and the third stirring rollers decrease from the outside to the inside. Since the sizes decrease from the outside to the inside and are distributed in a circular array, the insoluble recombinant protein solution in the tank can be fully stirred at different positions and heights, and one end of the same vertical side of the first stirring roller, the second stirring roller and the third stirring roller with corresponding positions are all fixedly connected with a scraper.
[0009] Preferably, the conveying device includes a pump body, the bottom end of the pump body is fixedly connected to the top of the bottom plate, the top side of the bottom plate is fixedly connected to a storage tank, the input end of the pump body is fixedly connected to a liquid outlet pipe, one end of the liquid outlet pipe is fixedly connected through the lower part of the outer surface of the tank body, the output end of the pump body is fixedly connected to a liquid inlet pipe, the pump body conveys the extracted solution to the storage tank through the liquid inlet pipe, one end of the liquid inlet pipe is fixedly connected through the lower part of the outer surface of the storage tank, the outer surface of the liquid inlet pipe is rotatably connected to a valve, and the delivery flow and on-off of the solution can be adjusted by controlling the valve, the lower end of the valve is movably penetrated and fixedly connected to a baffle on the outer surface of the liquid inlet pipe, and the outer surface of the baffle is in contact with the inner wall of the liquid inlet pipe.
[0010] Compared with the prior art, the beneficial effects of the present invention are:
[0011] 1. The utility model can fully stir the insoluble recombinant protein solution in the tank by rotating the shaft, and can scrape off the substances attached to the inner wall of the tank during the rotation process, thereby achieving the purpose of facilitating the mixing and recovery of the recombinant protein solution and facilitating the cleaning of the dirt on the inner wall of the tank;
[0012] 2. The utility model can adjust the delivery flow rate and on-off of the solution by controlling the valve. By adjusting the feed rate of the recombinant protein solution, the concentration change rate of the denaturant in the dialysis tank can be changed to minimize precipitation, thereby achieving the purpose of facilitating the adjustment of the feed rate and reducing precipitation. BRIEF DESCRIPTION OF THE DRAWINGS
[0013] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.
[0014] Figure 1 This is a schematic structural diagram of the utility model.
[0015] Figure 2 This is a schematic diagram of the mixing device of the present invention.
[0016] Figure 3 This is a schematic diagram of the conveying device of the present utility model.
[0017] In the figure: 1. bottom plate; 2. conveying device; 3. mixing device; 4. discharge pipe; 5. discharge valve; 21. pump body; 22. storage tank; 23. tank cover; 24. handle; 25. liquid outlet pipe; 26. liquid inlet pipe; 27. valve; 28. baffle; 31. tank body; 32. rotating shaft; 33. support plate; 34. motor; 35. propeller; 36. first stirring roller; 37. second stirring roller; 38. third stirring roller; 39. scraper. DETAILED DESCRIPTION
[0018] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.
[0019] Example: Figure 1-3As shown, the utility model provides an insoluble recombinant protein recovery device, including a bottom plate 1, a conveying device 2 is fixedly connected to one side of the top of the bottom plate 1, a mixing device 3 is fixedly connected to the other side of the top of the bottom plate 1, the mixing device 3 includes a tank body 31, the bottom end of the tank body 31 is fixedly connected to the top of the bottom plate 1, the outer surface of the tank body 31 is penetrated and connected to the drain pipe 4, the outer surface of the drain pipe 4 is rotatably connected to the drain valve 5, the upper and lower inner walls of the tank body 31 are rotatably connected to the rotating shaft 32, the top of the tank body 31 is fixedly connected to a support plate 33, the upper inner wall of the support plate 33 is fixedly connected to a motor 34, and the output end of the motor 34 penetrates the top of the tank body 31 and is fixedly connected to the top of the rotating shaft 32, the outer surface of the rotating shaft 32 is fixedly sleeved with a propeller 35, and when the rotating shaft 32 rotates, the propeller 35 is driven to rotate. The solution in the tank body 31 can be made to flow in an up-and-down direction, thereby further enhancing the stirring effect, ensuring that the various components in the solution are fully mixed, and promoting the recovery of insoluble recombinant proteins. A plurality of first stirring rollers 36 are fixedly connected to the outer surface of the rotating shaft 32, a plurality of second stirring rollers 37 are fixedly connected to the outer surface of the rotating shaft 32, and a plurality of third stirring rollers 38 are fixedly connected to the outer surface of the rotating shaft 32. The plurality of first stirring rollers 36, the second stirring rollers 37 and the third stirring rollers 38 are all distributed in a ring array, and the sizes of the plurality of first stirring rollers 36, the second stirring rollers 37 and the third stirring rollers 38 decrease from the outside to the inside. One end of the same vertical side of the first stirring rollers 36, the second stirring rollers 37 and the third stirring rollers 38 in corresponding positions are all fixedly connected to a scraper 39, and one end of the plurality of scrapers 39 is in contact with the inner wall of the tank body 31;
[0020] The rotating shaft 32 rotates, and the multiple first stirring rollers 36, second stirring rollers 37 and third stirring rollers 38 on the rotating shaft 32 rotate accordingly to fully stir the insoluble recombinant protein solution in the tank body 31. The rotating shaft 32 rotates, and during the rotation process, the material attached to the inner wall of the tank body 31 can be scraped off to prevent the insoluble recombinant protein or other components from accumulating on the inner wall of the tank body 31, thereby facilitating the mixing of the recombinant protein solution to promote recovery and facilitating the cleaning of the dirt on the inner wall of the tank body 2.
[0021] The conveying device 2 includes a pump body 21, the bottom end of the pump body 21 is fixedly connected to the top of the bottom plate 1, a storage tank 22 is fixedly connected to one side of the top of the bottom plate 1, a tank cover 23 is placed on the top of the storage tank 22, and a handle 24 is fixedly connected to the top of the tank cover 23. The input end of the pump body 21 is fixedly connected to a liquid outlet pipe 25, and the pump body 21 conveys the extracted solution to the storage tank 22 through the liquid inlet pipe 26, one end of the liquid outlet pipe 25 is fixedly connected to the lower part of the outer surface of the tank body 31, and the output end of the pump body 21 is fixedly connected to the liquid inlet pipe 26, one end of the liquid inlet pipe 26 is fixedly connected to the lower part of the outer surface of the storage tank 22, and the outer surface of the liquid inlet pipe 26 is rotatably connected to a valve 27, and the delivery flow and on-off of the solution can be adjusted by controlling the valve 27. The lower end of the valve 27 is movably penetrated and fixedly connected to the outer surface of the liquid inlet pipe 26, and the outer surface of the baffle 28 is in contact with the inner wall of the liquid inlet pipe 26;
[0022] The pump body 21 delivers the extracted solution to the storage tank 22 through the liquid inlet pipe 26. The delivery flow rate and on-off of the solution can be adjusted by controlling the valve 27. By adjusting the feed rate of the recombinant protein solution, the change rate of the denaturant concentration in the dialysis tank can be changed to minimize precipitation, making it easy to adjust the feed rate to reduce precipitation.
[0023] Working principle: After the motor 34 is started, it drives the shaft 32 to rotate, and the multiple first stirring rollers 36, second stirring rollers 37 and third stirring rollers 38 on the shaft 32 rotate accordingly. Since the sizes decrease from the outside to the inside and are distributed in a circular array, the insoluble recombinant protein solution in the tank 31 can be fully stirred at different positions and heights. When the shaft 32 rotates, it drives the propeller 35 to rotate. The propeller 35 can make the solution in the tank 31 flow in the up and down directions, further enhancing the stirring effect and ensuring that the various components in the solution are fully mixed. To promote the recovery of insoluble recombinant protein, a scraper 39 is connected to one end of the same vertical side of the first stirring roller 36, the second stirring roller 37 and the third stirring roller 38 in corresponding positions. As the rotating shaft 32 rotates, one end of the scraper 39 is in contact with the inner wall of the tank body 31. During the rotation process, it can scrape off the material attached to the inner wall of the tank body 31, preventing the accumulation of insoluble recombinant protein or other components on the inner wall of the tank body 31, thereby achieving the purpose of facilitating the mixing of the recombinant protein solution to promote recovery and facilitating the cleaning of the dirt on the inner wall of the tank body 31;
[0024] The pump body 21 extracts the mixed solution in the tank body 31 through the liquid outlet pipe 25. One end of the liquid outlet pipe 25 is fixedly connected to the lower part of the outer surface of the tank body 31, and can extract the solution at a lower position in the tank body 31 to ensure comprehensive solution extraction. The pump body 21 transports the extracted solution to the storage tank 22 through the liquid inlet pipe 26. The outer surface of the liquid inlet pipe 26 is rotatably connected to a valve 27. By controlling the valve 27, the delivery flow and on-off of the solution can be adjusted. The baffle 28 connected to the output end of the valve 27 fits into the inner wall of the liquid inlet pipe 26, which can play a better sealing role when the valve 27 is closed to prevent solution leakage. When the solution in the storage tank 22 needs to be transported to the tank body 31 again for further processing, it can be achieved by reverse operation of the pump body 21. By adjusting the feed rate of the recombinant protein solution, the concentration change rate of the denaturant in the dialysis tank can be changed to minimize precipitation, thereby achieving the purpose of facilitating the adjustment of the feed rate and reducing precipitation.
[0025] Obviously, those skilled in the art may make various modifications and variations to the present invention without departing from the spirit and scope of the present invention. Thus, if such modifications and variations fall within the scope of the claims of the present invention and their equivalents, the present invention is intended to include such modifications and variations.
Claims
1. An insoluble recombinant protein recovery device, comprising a bottom plate (1), characterized in that: A conveying device (2) is fixedly connected to one side of the top of the bottom plate (1), and a mixing device (3) is fixedly connected to the other side of the top of the bottom plate (1); The mixing device (3) comprises a tank body (31), the bottom end of the tank body (31) is fixedly connected to the top end of the bottom plate (1), the upper and lower inner walls of the tank body (31) are rotatably connected to a rotating shaft (32), the top end of the tank body (31) is fixedly connected to a support plate (33), the upper inner wall of the support plate (33) is fixedly connected to a motor (34), and the output end of the motor (34) passes through the top end of the tank body (31) and is fixedly connected to the top end of the rotating shaft (32), the outer surface of the rotating shaft (32) is fixedly connected to a plurality of first stirring rollers (36), the outer surface of the rotating shaft (32) is fixedly connected to a plurality of second stirring rollers (37), and the outer surface of the rotating shaft (32) is fixedly connected to a plurality of third stirring rollers (38), and one end of the same vertical side of the first stirring roller (36), the second stirring roller (37) and the third stirring roller (38) corresponding to each other are fixedly connected to a scraper (39).
2. The insoluble recombinant protein resuscitation device according to claim 1, characterized in that: The conveying device (2) includes a pump body (21), the bottom end of the pump body (21) is fixedly connected to the top end of the bottom plate (1), and the top side of the bottom plate (1) is fixedly connected to a storage tank (22). The input end of the pump body (21) is fixedly connected to a liquid outlet pipe (25), and one end of the liquid outlet pipe (25) is fixedly connected to the lower part of the outer surface of the tank body (31). The output end of the pump body (21) is fixedly connected to a liquid inlet pipe (26), and one end of the liquid inlet pipe (26) is fixedly connected to the lower part of the outer surface of the storage tank (22). The outer surface of the liquid inlet pipe (26) is rotatably connected to a valve (27), and the lower end of the valve (27) is movably connected to the outer surface of the liquid inlet pipe (26) and fixedly connected to a baffle (28).
3. The insoluble recombinant protein resuscitation device according to claim 1, characterized in that: The outer surface of the tank body (31) is connected to a drain pipe (4) through which a drain valve (5) is rotatably connected.
4. The insoluble recombinant protein resuscitation device according to claim 1, characterized in that: A propeller (35) is fixedly sleeved on the outer surface of the rotating shaft (32).
5. The insoluble recombinant protein resuscitation device according to claim 1, characterized in that: One end of each of the scrapers (39) is in contact with the inner wall of the tank body (31).
6. The insoluble recombinant protein resuscitation device according to claim 1, characterized in that: The plurality of first stirring rollers (36), second stirring rollers (37) and third stirring rollers (38) are distributed in a ring array, and the sizes of the plurality of first stirring rollers (36), second stirring rollers (37) and third stirring rollers (38) decrease from the outside to the inside.
7. The insoluble recombinant protein resuscitation device according to claim 2, characterized in that: A tank cover (23) is placed on the top of the storage tank (22), and a handle (24) is fixedly connected to the top of the tank cover (23).
8. The insoluble recombinant protein resuscitation device according to claim 2, characterized in that: The outer surface of the baffle (28) is in contact with the inner wall of the liquid inlet pipe (26).