Mesenchymal stem cell culture dish

Abstract

The utility model discloses a mesenchymal stem cell culture dish which comprises an upper cylinder and a lower cylinder, the upper surface of the upper cylinder and the upper surface of the lower cylinder are both in threaded connection with a first fixing ring, a rotating ring is arranged above the first fixing ring, a second fixing ring is arranged above the rotating ring, rotating grooves are formed in the first fixing ring and the second fixing ring, rotating blocks are rotationally connected in the rotating grooves, and the rotating blocks are arranged in the rotating grooves. The rotating ring is fixedly connected to the side surface of the connecting column, a fixing column is fixedly connected to the side surface of the rotating ring, a quantitative cylinder is fixedly connected to the end, away from the rotating ring, of the fixing column, a liquid injection pipe and a liquid conveying pipe are fixedly connected to the upper end and the lower end of the quantitative cylinder correspondingly, and valves are arranged on the liquid injection pipe and the liquid conveying pipe correspondingly; the ends, away from the sealing plate, of the liquid injection pipes are fixedly connected with first flow division pipes and located at the two ends of the first flow division pipes respectively, and the middle sections of the first flow division pipes are connected with feeding pipes. By means of the structure, the same culture media can be injected into different mesenchymal stem cell culture dishes respectively, and experiment repeatability can be improved conveniently.

Description

Technical Field

[0001] The utility model relates to the technical field of mesenchymal stem cell culture dishes, in particular to a mesenchymal stem cell culture dish. Background Art

[0002] Existing mesenchymal stem cell culture dishes are provided with annular guide grooves and connecting grooves to reduce the impact of culture fluid falling into the culture dish. However, when in use, the same culture medium cannot be injected into multiple different mesenchymal stem cell culture dishes, which is not convenient for improving the repeatability of the experiment, and is even more inconvenient for comparing their growth rates, morphological changes, and differentiation potential. For example, a Chinese patent discloses "A Primary Mesenchymal Stem Cell Culture Dish", and its application number is: "CN202222485671.4". It is provided with annular guide grooves and connecting grooves to reduce the impact of culture fluid falling into the culture dish. However, when in use, the same culture medium cannot be injected into multiple different mesenchymal stem cell culture dishes, which is not convenient for improving the repeatability of the experiment, and is even more inconvenient for comparing their growth rates, morphological changes, and differentiation potential, and has limitations in use. Utility Model Content

[0003] The purpose of the present invention is to solve at least one of the technical problems existing in the prior art and to provide a mesenchymal stem cell culture dish. The dish comprises an upper tube and a lower tube, a first fixing ring, a rotating ring, a second fixing ring, a rotating groove, a rotating block, a connecting column, a fixing column, a quantitative tube, an injection tube and an infusion tube, a valve, a first shunt tube, and a feeding tube. The dish can be used to inject the same culture medium into different mesenchymal stem cell culture dishes, thereby improving the repeatability of experiments and making it easier to compare their growth rates, morphological changes, differentiation potentials, and other characteristics. By observing the responses of different cell lines to the same culture medium, the formula of the culture medium can be optimized to meet the needs of specific cell lines. Simultaneous processing of multiple cell lines can improve experimental efficiency and save time and culture medium usage.

[0004] The utility model also provides the above-mentioned mesenchymal stem cell culture dish, comprising: an upper tube and a lower tube, the upper tube and the lower tube having the same structure, and the upper surfaces of both being threadedly connected with a fixing ring 1, a rotating ring being provided above the fixing ring 1, a fixing ring 2 being provided above the rotating ring, and a rotating groove being provided in both the fixing ring 1 and the fixing ring 2, a rotating block being rotatably connected in the rotating groove, the two rotating blocks being connected via a connecting column, the rotating ring being fixedly connected to the side surface of the connecting column, the side surface of the rotating ring being fixedly connected to a fixing column, the fixing column being fixedly connected to a quantitative cylinder at one end away from the rotating ring, the upper and lower ends of the quantitative cylinder being fixedly connected to an injection tube and an infusion tube, respectively, and the injection tube Valves are provided on the infusion tubes, and the two infusion tubes are respectively connected to the upper tube and the lower tube. The end of the injection tube away from the sealing plate is fixedly connected to the shunt tube 1, and is respectively located at both ends of the shunt tube 1. The middle section of the shunt tube 1 is connected to the feeding tube. Through the above structure, the same culture medium can be injected into different mesenchymal stem cell culture dishes, which is convenient for improving the repeatability of the experiment and making it easier to compare their growth rate, morphological changes, differentiation potential and other characteristics. By observing the response of different cell lines to the same culture medium, it can help optimize the formula of the culture medium to meet the needs of specific cell lines. Processing multiple cell lines at the same time can improve experimental efficiency and save time and culture medium usage.

[0005] According to the mesenchymal stem cell culture dish described in the utility model, the lower surfaces of the upper tube and the lower tube are fixedly connected to the bottom plate 1 and the bottom plate 2 respectively, and the lower surface of the bottom plate is provided with a card slot. The above structure facilitates use.

[0006] According to the mesenchymal stem cell culture dish of the present invention, a sealing plate is fixedly connected to the upper surface of the second fixing ring. The sealing plate has the same diameter as the upper tube and the lower tube. The above structure facilitates sealing.

[0007] According to the mesenchymal stem cell culture dish described in the utility model, a clamping ring is fixedly connected to the upper surface of the sealing plate on the lower tube, and the clamping ring is threadedly connected to the clamping groove. Through the above structure, it is easy to connect with the upper tube above.

[0008] According to the mesenchymal stem cell culture dish described in the utility model, the upper tube and the lower tube are both threadedly connected with a threaded ring, and the bottom end of the threaded ring is fixedly connected to a liquid collecting bucket. Through the above structure, it is convenient to fix the liquid collecting bucket, thereby concentrating the culture medium and facilitating the adjustment of its position.

[0009] According to the mesenchymal stem cell culture dish described in the present invention, the bottom end of the liquid collecting bucket is fixedly connected to a liquid outlet pipe 1, and the end of the liquid outlet pipe 1 away from the liquid collecting bucket is fixedly connected to a diversion pipe 2, and is located in the middle section of the diversion pipe 2. Through the above structure, the culture medium is easily transported into the culture dish, and its movement is buffered to avoid affecting the cells.

[0010] According to the mesenchymal stem cell culture dish described in the present invention, both ends of the second diversion tube are fixedly connected to connecting tubes, and the other ends of the two connecting tubes are fixedly connected to a converging tube. Through the above structure, the culture medium can be easily concentrated.

[0011] According to the mesenchymal stem cell culture dish described in the present invention, the side surface of the confluence pipe is fixedly connected to the second liquid outlet pipe, and the second liquid outlet pipe is located in the middle section of the confluence pipe and is connected to the confluence pipe. Through the above structure, the culture medium is easily transported into the culture dish.

[0012] Beneficial effects:

[0013] Compared with the existing technology, this mesenchymal stem cell culture dish is equipped with an upper tube and a lower tube, a fixed ring 1, a rotating ring, a fixed ring 2, a rotating groove, a rotating block, a connecting column, a fixed column, a quantitative cylinder, an injection tube and an infusion tube, a valve, a shunt tube 1, and a feeding tube. The same culture medium can be injected into different mesenchymal stem cell culture dishes respectively, which facilitates improving the repeatability of the experiment and makes it easier to compare their growth rates, morphological changes, differentiation potential and other characteristics. By observing the responses of different cell lines to the same culture medium, it can help optimize the formula of the culture medium to meet the needs of specific cell lines. Processing multiple cell lines at the same time can improve experimental efficiency and save time and culture medium usage. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The present invention will be further described below with reference to the accompanying drawings and embodiments;

[0015] Figure 1 This is the overall structure diagram of a mesenchymal stem cell culture dish of the utility model;

[0016] Figure 2 This is a bottom-up structural diagram of a mesenchymal stem cell culture dish of the present invention;

[0017] Figure 3 This is a transverse cross-sectional structural diagram of a mesenchymal stem cell culture dish of the present invention;

[0018] Figure 4 This is a longitudinal cross-sectional structural diagram of a mesenchymal stem cell culture dish of the present invention;

[0019] Figure 5 The utility model is a mesenchymal stem cell culture dish Figure 3Enlarged structural diagram at point C in the middle.

[0020] Legend:

[0021] 1. Upper tube; 2. Bottom plate 1; 3. Lower tube; 4. Bottom plate 2; 5. Fixed ring 1; 6. Rotating ring; 7. Fixed ring 2; 8. Sealing plate; 9. Feed pipe; 10. Diverter pipe 1; 11. Liquid injection pipe; 12. Measuring cylinder; 13. Valve; 14. Liquid infusion pipe; 15. Fixed column; 16. Threaded ring; 17. Liquid collecting hopper; 18. Liquid outlet pipe 1; 19. Diverter pipe 2; 20. Connecting pipe; 21. Clamping groove; 22. Clamping ring; 23. Rotating groove; 24. Rotating block; 25. Connecting column; 26. Junction pipe; 27. Liquid outlet pipe. DETAILED DESCRIPTION

[0022] This section will describe in detail the specific embodiments of the present invention. The preferred embodiments of the present invention are shown in the accompanying drawings. The purpose of the accompanying drawings is to supplement the description of the text part of the specification with graphics, so that people can intuitively and vividly understand each technical feature and overall technical solution of the present invention, but it cannot be understood as a limitation on the scope of protection of the present invention.

[0023] Reference Figure 1-5 The embodiment of the present invention is a mesenchymal stem cell culture dish, which includes: an upper tube 1 and a lower tube 3. The upper tube 1 and the lower tube 3 have the same structure, and the upper surface is threadedly connected with a fixing ring 5, which is convenient for connecting and fixing the fixing ring 5. A rotating ring 6 is provided above the fixing ring 5, and a fixing ring 2 7 is provided above the rotating ring 6. A rotating groove 23 is provided in the fixing ring 5 and the fixing ring 2 7. A rotating block 24 is rotatably connected in the rotating groove 23 to facilitate the rotation of the rotating block 24. The two rotating blocks 24 are connected by a connecting column 25 to facilitate connection. The rotating ring 6 is fixedly connected to the side surface of the connecting column 25 to facilitate the rotation of the rotating ring 6. The side of the rotating ring 6 A fixed column 15 is fixedly connected to the surface, and the fixed column 15 is fixedly connected to a metering cylinder 12 at one end away from the rotating ring 6 to fix the metering cylinder 12. The upper and lower ends of the metering cylinder 12 are respectively fixedly connected with an injection tube 11 and a transfusion tube 14. The injection tube 11 and the transfusion tube 14 are fixed, and valves 13 are provided on the injection tube 11 and the transfusion tube 14 to control the injection and output of the culture medium. The two transfusion tubes 14 are respectively connected to the upper tube 1 and the lower tube 3. The injection tube 11 is fixedly connected to a shunt tube 10 at one end away from the sealing plate 8 for easy diversion, and the shunt tube 10 is located at both ends. The middle section of the shunt tube 10 is connected with a feed tube 9 for easy injection of the culture medium.

[0024] The upper tube 1 and the lower tube 3 are both threadedly connected with a threaded ring 16, and the bottom end of the threaded ring 16 is fixedly connected to a liquid collecting bucket 17 to concentrate the culture medium. The bottom end of the liquid collecting bucket 17 is fixedly connected to a liquid outlet pipe 18, and the liquid outlet pipe 18 is fixedly connected to a diversion pipe 2 19 at one end away from the liquid collecting bucket 17, and is located in the middle of the diversion pipe 2 19 to divert the culture medium. Both ends of the diversion pipe 2 19 are fixedly connected to a connecting pipe 20, and the other end of the two connecting pipes 20 is fixedly connected to a confluence pipe 26 for merging. The side surface of the confluence pipe 26 is fixedly connected to the liquid outlet pipe 2 2 7. The second liquid outlet pipe 27 is located in the middle section of the confluence pipe 26 and is connected to the confluence pipe 26 to facilitate the transportation of culture medium. The lower surfaces of the upper tube 1 and the lower tube 3 are fixedly connected with the bottom plate 1 2 and the bottom plate 2 4 respectively. The lower surface of the bottom plate 1 2 is provided with a card groove 21 to facilitate connection with the card ring 22. The upper surface of the fixing ring 2 7 is fixedly connected with a sealing plate 8. The sealing plate 8 has the same diameter as the upper tube 1 and the lower tube 3 to facilitate sealing. The upper surface of the sealing plate 8 on the lower tube 3 is fixedly connected with a card ring 22. The card ring 22 is threadedly connected to the card groove 21 to facilitate connection and fixation.

[0025] Working principle: When in use, by observing the transparent upper tube 1 and lower tube 3, adjust the position of the threaded ring 16 so that the distance between the liquid outlet pipe 27 and the bottom of the upper tube 1 and lower tube 3 is at an appropriate position. Then, connect the fixed ring 15 and the various devices above it to the upper tube 1 and lower tube 3, and inject the culture medium through the feed pipe 9. Under the action of the injection pipe 11, it flows into the quantitative cylinder 12, and through the measurement of the quantitative cylinder 12, the opening and closing of the valve 13 is controlled to make the quantified culture medium flow into the collecting hopper 17, and through the buffering of the liquid outlet pipe 18, the diversion pipe 2 19, the connecting pipe 20, the confluence pipe 26, and the liquid outlet pipe 27, it flows into the upper tube 1 and the lower tube 3.

[0026] The embodiments of the present invention are described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments. Various changes can be made within the scope of knowledge possessed by ordinary technicians in the relevant technical field without departing from the purpose of the present invention.

Claims

1. A mesenchymal stem cell culture dish, characterized in that: include: The upper tube (1) and the lower tube (3) have the same structure, and the upper surface of the upper tube (1) and the lower tube (3) are both threadedly connected with a fixing ring (5), a rotating ring (6) is provided above the fixing ring (5), and a fixing ring (7) is provided above the rotating ring (6), and a rotating groove (23) is provided in the fixing ring (5) and the fixing ring (7), and a rotating block (24) is rotatably connected in the rotating groove (23), and the two rotating blocks (24) are connected by a connecting column (25), and the rotating ring (6) is fixedly connected to the side surface of the connecting column (25), and the side surface of the rotating ring (6) is fixedly connected. A fixed column (15) is connected, and the fixed column (15) is fixedly connected to a quantitative cylinder (12) at one end away from the rotating ring (6). The upper and lower ends of the quantitative cylinder (12) are respectively fixedly connected to an injection tube (11) and a liquid infusion tube (14), and valves (13) are provided on the injection tube (11) and the liquid infusion tube (14). The two liquid infusion tubes (14) are respectively connected to the interior of the upper cylinder (1) and the lower cylinder (3). The injection tube (11) is fixedly connected to a diversion tube (10) at one end away from the sealing plate (8), and the diversion tubes are respectively located at the two ends of the diversion tube (10). The middle section of the diversion tube (10) is connected to a feed pipe (9).

2. The mesenchymal stem cell culture dish according to claim 1, characterized in that: The lower surfaces of the upper tube (1) and the lower tube (3) are fixedly connected to a bottom plate 1 (2) and a bottom plate 2 (4) respectively, and a clamping groove (21) is provided on the lower surface of the bottom plate 1 (2).

3. The mesenchymal stem cell culture dish according to claim 1, characterized in that: A sealing plate (8) is fixedly connected to the upper surface of the second fixing ring (7), and the sealing plate (8) has the same diameter as the upper cylinder (1) and the lower cylinder (3).

4. The mesenchymal stem cell culture dish according to claim 3, characterized in that: A snap ring (22) is fixedly connected to the upper surface of the sealing plate (8) on the lower cylinder (3), and the snap ring (22) is threadedly connected to the clamping groove (21).

5. The mesenchymal stem cell culture dish according to claim 4, characterized in that: The upper cylinder (1) and the lower cylinder (3) are both threadedly connected with a threaded ring (16), and the bottom end of the threaded ring (16) is fixedly connected with a liquid collecting bucket (17).

6. The mesenchymal stem cell culture dish according to claim 5, characterized in that: The bottom end of the liquid collecting hopper (17) is fixedly connected to a liquid outlet pipe 1 (18), and the end of the liquid outlet pipe 1 (18) away from the liquid collecting hopper (17) is fixedly connected to a diversion pipe 2 (19) and is located in the middle section of the diversion pipe 2 (19).

7. The mesenchymal stem cell culture dish according to claim 6, characterized in that: Both ends of the second diversion pipe (19) are fixedly connected to a connecting pipe (20), and the other ends of the two connecting pipes (20) are fixedly connected to a converging pipe (26).

8. The mesenchymal stem cell culture dish according to claim 7, characterized in that: A second liquid outlet pipe (27) is fixedly connected to the side surface of the confluence pipe (26), and the second liquid outlet pipe (27) is located in the middle section of the confluence pipe (26) and is communicated with the confluence pipe (26).

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