Aspermia and oligospermia chromosome variation detection kit

Abstract

The utility model relates to the technical field of biological detection, and provides an azoospermia chromosome variation detection kit which comprises a kit body with a cavity and a reagent tube placed in the kit body, the top of the kit body is provided with an opening, the kit body is provided with a cover plate used for opening and closing the opening, and the reagent tube is arranged in the cavity. Thermal insulation layers are arranged on the inner peripheral wall and the bottom wall of the kit body, a thermal insulation layer is also arranged on the surface, close to the opening, of the cover plate, and a temperature control assembly is arranged in the kit; placing plates are arranged in the cavities, through holes allowing reagent tubes to penetrate through are formed in the placing plates in a penetrating mode, and the multiple placing plates are arranged in the height direction of the kit body at intervals. The method has the beneficial effect of reducing the influence on the detection result.

Description

Technical Field

[0001] The present application relates to the technical field of biological detection, and in particular to a kit for detecting chromosome mutations in azoospermia and oligospermia. Background Art

[0002] Infertility is a global health issue caused by multiple factors. It is estimated that nearly half of all infertility cases are due to male infertility and genetic factors, particularly microdeletions. Y chromosome microdeletions are the primary cause of over 15% of male infertility. Primary azoospermia and oligospermia are both major manifestations of infertility. Oligospermia is generally caused by spermatogenesis disorders or inflammation of the reproductive organs, while azoospermia is the absence of sperm in semen or problems with sperm production or output.

[0003] Therefore, early screening for Y chromosome microdeletions in male infertility patients is crucial. This not only helps clarify the true cause of the condition, avoiding unnecessary testing and ineffective treatment, but also helps predict or prevent the transmission of genetic defects. In existing technology, patients typically collect samples themselves into test tubes. Staff then use a pipette to extract the appropriate amount of sample into different reagent tubes, which are then placed in a test kit for temporary storage. After multiple patient samples are collected, the test kits are transferred to a laboratory for testing using relevant equipment to determine whether the patient has azoospermia or oligospermia.

[0004] During the testing process, the test tube needs to be tested at room temperature of 25 to 30 degrees or a specific temperature to ensure the accuracy of the test. However, with the change of seasons and in different regions, the external temperature changes and the relative difference from the room temperature are relatively large, which may affect the temperature inside the test kit and the test tube, thereby affecting the subsequent test results. Therefore, further improvement is needed. Utility Model Content

[0005] In order to reduce the impact on the test results, the present application provides a chromosome variation detection kit for azoospermia and oligospermia.

[0006] The present application provides a kit for detecting chromosome mutations in azoospermia and oligospermia, which adopts the following technical solution:

[0007] A kit for detecting chromosomal variations in azoospermia and oligospermia comprises a kit body having a chamber and a reagent tube placed in the kit body, wherein the top of the kit body is open, the kit body is provided with a cover for opening and closing the opening, the inner peripheral wall and the bottom wall of the kit body are both provided with an insulation layer, the surface of the cover close to the opening is also provided with an insulation layer, and a temperature control component is provided in the kit; a placement plate is provided in the chamber, the placement plate is penetrated by a through hole for the reagent tube to pass through, and a plurality of placement plates are provided at intervals along the height direction of the kit body.

[0008] By adopting the above technical solution, the temperature of the reagent kit body is controlled as needed by providing a heat-insulating layer and a temperature-control component. This reduces the possibility of significant temperature fluctuations in the chamber when the cover is opened when the reagent tubes are subsequently placed in the reagent kit body. The heat-insulating layer can also reduce the impact of external temperature on the temperature of the chamber during transportation. The placement plate is provided for the placement of reagent tubes, and multiple placement plates are provided to increase the number of reagent tubes that can be placed.

[0009] Preferably, the temperature control component includes a semiconductor refrigerator and a temperature sensor electrically connected to the semiconductor refrigerator, the temperature sensor is electrically connected to a controller, the controller is electrically connected to the semiconductor refrigerator, the placement plate is made of a thermally conductive metal material, and the placement plate abuts against the semiconductor refrigerator.

[0010] By adopting the above technical solution, a semiconductor refrigerator is used, which utilizes the thermo-electric effect of semiconductors to generate cooling energy, also known as a thermoelectric refrigerator. A conductor is used to connect two pieces of different metals. When direct current is turned on, the temperature at one connection point decreases and the temperature at the other connection point increases, thereby increasing or decreasing the temperature of the placement plate. Since the placement plate is made of a thermally conductive metal material, the heat conduction of the placement plate can be used to heat or cool the reagent tube. The temperature in the chamber is then monitored by a temperature sensor. When the required temperature is reached, the corresponding temperature is fed back to the controller for control, and the semiconductor refrigerator is stopped.

[0011] Preferably, the through holes provided on the plurality of placement plates are staggered, the reagent tubes are in contact with the placement plate below, and the upper surface of the placement plate is provided with positioning grooves for the reagent tubes to be slidably inserted.

[0012] By adopting the above technical solution, the reagent tube is passed through the perforation to improve the placement of the reagent tube. In order to reduce the possibility of shaking of the reagent tube during transportation and collision with adjacent actual tubes, a positioning groove is provided on the upper surface of the placement plate located below the reagent tube to position the lower end of the reagent tube, thereby reducing the possibility of breakage of the reagent tube made of glass material.

[0013] Preferably, several of the upper surfaces of the placement plates in the middle are protruding with connecting rods, the length direction of the connecting rods is perpendicular to the upper surface of the placement plates, the upper end face of the connecting rod located at the top is detachably connected to a cross bar, the cross bar abuts against the lower surface of the cover plate, the upper end face of the reagent tube is installed with an end cover, and the aperture of the through hole is smaller than the outer diameter of the end cover.

[0014] By adopting the above technical solution, connecting rods are set to limit the spacing between adjacent placement plates, so as to reduce the impact of the placement plates slipping and detaching on the reagent tubes. In addition, through the detachable connected cross bar, after being transported to the laboratory, the entire placement plate can be lifted through the cross bar to reduce the difficulty in picking up the placement plate. In addition, end caps are provided on the reagent tubes to reduce the possibility of the reagent tubes slipping and detaching from the placement plate during the process of lifting the entire placement plate.

[0015] Preferably, the lower surface of the cross bar is provided with a first slot for the connecting rod to be slidably inserted, the connecting rod is penetrated by a limiting hole, the cross bar is provided with a limiting rod that is slidably inserted into the first slot, and the limiting rod is slidably inserted into the limiting hole.

[0016] By adopting the above technical solution, the limiting rod slides through the first slot and the limiting hole so as to lift up the placement plate connected to the connecting rod. Since several placement plates are provided, several placement plates can be taken out one by one according to needs.

[0017] Preferably, the lower surface of the placement plate is provided with a second slot for inserting the connecting rod.

[0018] By adopting the above technical solution, a second slot for inserting the connecting rod is provided to limit the adjacent placement plates, thereby reducing the possibility of relative shaking of the placement plates.

[0019] Preferably, a spring is coaxially sleeved on the outer peripheral wall of the limiting rod, and the spring forces the limiting rod to move in the direction of the limiting hole. A through slot connected to the first slot is opened on the side wall of the cross bar, and the limiting rod is passed through the through slot, and the spring is built into the through slot. A mounting plate for spring installation is built into the through slot, and both ends of the spring are respectively fixedly connected to the inner wall of the through slot and the surface of the mounting plate close to the first slot, and the limiting rod is passed through the mounting plate.

[0020] By adopting the above technical solution, a spring is provided. Since the spring forces the limit rod to move toward the limit hole, when the limit rod is slid through the groove and the limit hole, the possibility of the limit rod slipping out of the limit hole can be reduced when the cross bar is lifted. It is also relatively simple compared to the process of the limit rod sliding through the groove and the limit hole.

[0021] Preferably, the connecting rod is slidably provided through a plurality of the placement plates, and a limiting member for limiting the sliding of the placement plates is provided on the connecting rod.

[0022] By adopting the above technical solution, the connecting rod is slidably passed through several placement plates, and the sliding of the placement plates is restricted by limiting pieces. The plates can be lifted at one time, and the distance between two adjacent placement plates can be adjusted to adapt to the placement of reagent tubes in different height directions.

[0023] In summary, the present invention has the following beneficial effects:

[0024] By providing an insulation layer and temperature control components, the temperature inside the test kit body can be controlled as needed to reduce the possibility of significant temperature changes in the chamber when the cover is opened when the test kit body is subsequently placed. The insulation layer can also reduce the impact of external temperature on the temperature inside the chamber during transportation. The placement plate is provided for the placement of test tubes, and multiple placement plates are provided to increase the number of test tubes that can be placed. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a schematic diagram of the overall structure of Example 1 of the present application;

[0026] Figure 2 This is a schematic diagram of the internal structure of Example 1 of the present application;

[0027] Figure 3 This is a schematic diagram of the structure of the placement plate in Example 1 of the present application;

[0028] Figure 4 This is a schematic structural diagram of the connecting rod and the crossbar in Example 1 of the present application;

[0029] Figure 5 It is a structural schematic diagram of the connecting rod in Example 2 of the present application.

[0030] Explanation of the accompanying drawings: 1. Reagent box body; 11. Chamber; 12. Cover plate; 121. Sealing ring; 13. Spring buckle; 2. Reagent tube; 21. End cap; 3. Placement plate; 31. Through hole; 32. Positioning groove; 33. Second slot; 4. Insulation layer; 5. Temperature control component; 51. Semiconductor refrigerator; 52. Temperature sensor; 6. Connecting rod; 61. Limiting hole; 62. Threaded hole; 7. Cross bar; 71. First slot; 72. Limiting rod; 73. Spring; 74. Through groove; 75. Mounting plate; 76. Force block; 8. Limiting member; 81. Limiting ring; 82. Limiting bolt. DETAILED DESCRIPTION

[0031] The following is combined with Figure 1-5 , further details of this application are given.

[0032] The present application discloses a kit for detecting chromosome mutations in azoospermia and oligospermia.

[0033] Example 1:

[0034] A kit for detecting chromosome variation in azoospermia and oligospermia, referring to Figure 1 、 Figure 2, comprising a reagent box body 1 having a chamber 11 and a reagent tube 2 placed in the reagent box body 1. In this embodiment, the reagent box body 1 can be provided in a cylindrical shape, a box shape, or other shapes, depending on the specific needs. In this embodiment, a box-shaped reagent box body 1 is specifically shown. The top of the reagent box body 1 is provided with an opening. The reagent box body 1 is provided with a cover plate 12 for opening and closing the opening. One end of the cover plate 12 is rotatably connected to one of the long sides of the reagent box body 1. A sealing ring 121 is provided on the upper surface of the cover plate 12 to reduce the possibility of outside air entering the chamber 11. The reagent box body 1 is provided with a spring buckle 13 for controlling the opening and closing of the cover plate 12.

[0035] Reference Figure 2 、 Figure 3 , wherein the upper end surface of the reagent tube 2 is installed with an end cap 21. A placement plate 3 is provided in the chamber 11, and a plurality of placement plates 3 are spaced apart along the height direction of the reagent reagent box body 1. The placement plates 3 are penetrated by a through hole 31 for the reagent tube 2 to pass through. The through holes 31 provided on the plurality of placement plates 3 are staggered. The lower end of the reagent tube 2 abuts against the placement plate 3 below, and the upper surface of the placement plate 3 is provided with a positioning groove 32 for the reagent tube 2 to slide and insert. It should be noted that the diameter of the lower end of the reagent tube 2 is smaller than the diameter of its own tube mouth, the diameter of the through hole 31 is smaller than the outer diameter of the end cap 21, and the diameter of the positioning groove 32 is smaller than the diameter of the pupil.

[0036] In this embodiment, the inner wall and the bottom wall of the reagent box body 1 are both provided with an insulation layer 4, and the surface of the cover plate 12 close to the opening is also provided with an insulation layer 4. A temperature control component 5 is provided in the reagent box body 1 to control the temperature of the placement plate 3. The temperature control component 5 specifically includes a semiconductor refrigerator 51 and a temperature sensor 52 electrically connected to the semiconductor refrigerator 51. The temperature sensor 52 is electrically connected to a controller (not shown in the figure), and the controller is electrically connected to the semiconductor refrigerator 51. The placement plate 3 is made of a metal material with thermal conductivity, specifically aluminum or copper, and the placement plate 3 is in contact with the semiconductor refrigerator 51. It should be noted that the temperature sensor 52 can be electrically connected to the placement plate 3 to monitor the temperature of the placement plate 3, and the relative temperature detection is more accurate.

[0037] Reference Figure 2 、 Figure 4Furthermore, in order to facilitate the removal of the placement plate 3 from the reagent box body 1, several placement plates 3 are provided with connecting rods 6 on the upper surfaces in the middle. The length direction of the connecting rods 6 is perpendicular to the upper surface of the placement plate 3. The upper end surface of the connecting rod 6 at the top is detachably connected to the cross bar 7. The cross bar 7 abuts against the lower surface of the cover plate 12. The lower surface of the cross bar 7 is provided with a first slot 71 for the connecting rod 6 to slide and insert. The connecting rod 6 is provided with a limiting hole 61. The cross bar 7 is provided with a limiting rod 72 that slides through the first slot 71. The limiting rod 72 is slidably inserted into the limiting hole 61. The outer peripheral wall of the limiting rod 72 is coaxially sleeved with a spring 73, which forces the limiting rod 72 to move toward the limiting hole 61. The side wall of the cross bar 7 is provided with a through slot 74 connected to the first slot 71. The limiting rod 72 is passed through the through slot 74, and the spring 73 is built into the through slot 74. The through slot 74 has a mounting plate 75 for mounting the spring 73. The two ends of the spring 73 are respectively fixedly connected to the inner wall of the through slot 74 and the surface of the mounting plate 75 close to the first slot 71, and the limiting rod 72 is passed through the mounting plate 75.

[0038] It should be noted that a force block 76 may be fixedly connected to the end surface of the limiting rod 72 exposed from the cross bar 7 to facilitate applying force to the limiting rod 72 .

[0039] Furthermore, in order to improve the stability between adjacent placement plates 3, in this embodiment, the lower surface of the placement plate 3 is provided with a second slot 33 for inserting the connecting rod 6.

[0040] The implementation principle of the azoospermia and oligospermia chromosome variation detection kit of the embodiment of the present application is: by providing an insulation layer 4 and a temperature control component 5, the temperature inside the kit body 1 is controlled according to demand, so as to reduce the possibility of a large impact of temperature changes in the chamber 11 when the reagent tube 2 is placed on the subsequent kit body 1 and the cover 12 is opened. In addition, the insulation layer 4 provided can reduce the influence of the external temperature on the temperature inside the chamber 11 during transportation.

[0041] Example 2:

[0042] Reference Figure 5 The difference from Example 1 is that several connecting rods 6 are connected into one connecting rod 6, and the connecting rod 6 is slidably passed through several placement plates 3. The connecting rod 6 is provided with a limiting member 8 for limiting the sliding of the placement plate 3. In this embodiment, the limiting member 8 includes a limiting ring 81 fixedly connected to the placement plate 3 and a limiting bolt 82 passed through the limiting ring 81. The limiting ring 81 is coaxially sleeved on the connecting rod 6. The connecting rod 6 is provided with a threaded hole 62 for threaded connection of the limiting bolt 82. Several threaded holes 62 are arranged at intervals along the length direction of the connecting rod 6.

[0043] It should be noted that the limiting member 8 may also include a limiting rod 72, a spring 73 and a force block 76 to speed up the adjustment of the placement plate 3, and the specific settings are based on needs.

[0044] The above are all preferred embodiments of the present application, and are not intended to limit the scope of protection of the present application. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the scope of protection of the present application.

Claims

1. A kit for detecting chromosome variation in azoospermia and oligospermia, characterized by: The invention comprises a reagent box body (1) having a chamber (11) and a reagent tube (2) placed in the reagent box body (1), wherein the top of the reagent box body (1) is open, and the reagent box body (1) is provided with a cover plate (12) for opening and closing the opening, the inner peripheral wall and the bottom wall of the reagent box body (1) are both provided with a heat-insulating layer (4), and the surface of the cover plate (12) close to the opening is also provided with a heat-insulating layer (4), and a temperature control component (5) is provided in the reagent box; a placement plate (3) is provided in the chamber (11), and a through hole (31) is provided through the placement plate (3) for the reagent tube (2) to pass through, and a plurality of placement plates (3) are provided at intervals along the height direction of the reagent box body (1).

2. A kit for detecting chromosome variation in azoospermia and oligospermia according to claim 1, characterized in that: The temperature control component (5) includes a semiconductor cooler (51) and a temperature sensor (52) electrically connected to the semiconductor cooler (51); the temperature sensor (52) is electrically connected to a controller; the controller is electrically connected to the semiconductor cooler (51); the placement plate (3) is made of a metal material with thermal conductivity; the placement plate (3) abuts against the semiconductor cooler (51).

3. A kit for detecting chromosome variation in azoospermia and oligospermia according to claim 1, characterized in that: The through holes (31) provided on the plurality of placement plates (3) are staggered, the reagent tube (2) abuts against the placement plate (3) below, and the upper surface of the placement plate (3) is provided with a positioning groove (32) for the reagent tube (2) to be slidably inserted.

4. A kit for detecting chromosome variation in azoospermia and oligospermia according to claim 3, characterized in that: A connecting rod (6) is protruding from the upper surface of the middle portion of several placement plates (3), and the length direction of the connecting rod (6) is perpendicular to the upper surface of the placement plate (3). The upper end surface of the connecting rod (6) located at the top is detachably connected to a cross bar (7), and the cross bar (7) abuts against the lower surface of the cover plate (12). The upper end surface of the reagent tube (2) is installed with an end cover (21), and the aperture of the through hole (31) is smaller than the outer diameter of the end cover (21).

5. The kit for detecting chromosome variation in azoospermia and oligospermia according to claim 4, wherein: The lower surface of the cross bar (7) is provided with a first slot (71) for the connecting rod (6) to be slidably inserted, the connecting rod (6) penetrates the limiting hole (61), the cross bar (7) is provided with a limiting rod (72) that is slidably inserted into the first slot (71), and the limiting rod (72) is slidably inserted into the limiting hole (61).

6. The kit for detecting chromosome variation in azoospermia and oligospermia according to claim 5, characterized in that: The lower surface of the placement plate (3) is provided with a second slot (33) for inserting the connecting rod (6).

7. The kit for detecting chromosome variation in azoospermia and oligospermia according to claim 5, characterized in that: The outer peripheral wall of the limiting rod (72) is coaxially sleeved with a spring (73), and the spring (73) forces the limiting rod (72) to move in the direction of the limiting hole (61). The side wall of the cross bar (7) is provided with a through groove (74) connected to the first slot (71), the limiting rod (72) is penetrated by the through groove (74), the spring (73) is built into the through groove (74), and the through groove (74) is built with a mounting plate (75) for mounting the spring (73), and the two ends of the spring (73) are respectively fixedly connected to the inner wall of the through groove (74) and the surface of the mounting plate (75) close to the first slot (71), and the limiting rod (72) is penetrated by the mounting plate (75).

8. The kit for detecting chromosome variation in azoospermia and oligospermia according to claim 4, characterized in that: The connecting rod (6) is slidably provided through a plurality of the placement plates (3), and a limiting member (8) for limiting the sliding of the placement plates (3) is provided on the connecting rod (6).

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