Sequencing batch reaction device for removing total DNA in sewage based on ferrate and calcium sulfite coupling system

Abstract

The utility model relates to the technical field of wastewater treatment, in particular to a sequencing batch reaction device for removing total DNA (Deoxyribose Nucleic Acid) in sewage based on a ferrate and calcium sulfite coupling system. The reaction device comprises an active species generation zone, a reaction zone and a precipitation zone. The active species generation area is provided with a potassium ferrate and calcium sulfite liquid inlet, a reaction liquid outlet and a controllable stirring paddle, and a hose communicated with the reaction area is arranged at the bottom of the active species generation area; the reaction area is provided with an acid-base liquid inlet, a pH probe, a submersible stirrer, a reaction liquid inlet, a sewage sample inlet and a liquid medicine outlet, and a hose communicated with the settling area is arranged at the bottom of the reaction area; and the settling zone is of a funnel-shaped structure. According to the sewage treatment device, potassium ferrate and calcium sulfite are ensured to be fully mixed in the active species reaction zone through effective partitioning, so that the utilization efficiency of a medicament is improved, the medicament and sewage are fully mixed in the reaction zone, pollutants are removed, flocculates are removed through gravity settling in the settling zone, and the total DNA in the sewage is effectively removed.

Description

Technical Field

[0001] The utility model relates to a batch reaction device for removing total DNA in sewage based on a ferrate and calcium sulfite coupling system, belonging to the technical field of wastewater treatment. Background Art

[0002] DNA in wastewater treatment plants primarily originates from human sewage, pharmaceutical wastewater, pet excreta, and environmental microorganisms. These sources contribute to the presence of DNA from diverse organisms. Certain types of DNA, such as antibiotic resistance genes, pathogen genes, and virulence genes, pose significant risks to the environment and public health. Transfer of this DNA into harmful bacteria in humans, animals, and plants can significantly increase public health risks. Therefore, the development of new devices to remove total DNA from wastewater is urgent.

[0003] At present, the main removal methods reported include biological, physical and chemical treatment technologies, but these methods have obvious limitations. Biological treatment technology has the following disadvantages: it cannot completely remove DNA, the treatment time is long, the environmental conditions are demanding, and it may cause microbial tolerance problems. The disadvantages of physical treatment technology are low treatment efficiency, high energy consumption, and high equipment maintenance costs. For example, after treatment with coagulation filtration, there is free DNA that cannot be intercepted by the membrane, and the ultrafiltration and microfiltration membranes need to be cleaned and replaced regularly, which increases the complexity and cost of equipment maintenance. Traditional disinfection methods also produce disinfection by-products that cause secondary damage to the environment and are greatly affected by water quality. For example, ozone disinfection will produce ketones, formaldehyde and other by-products that may have potential carcinogenic, mutagenic and reproductive toxicity to human health, and ozone disinfection will be affected by anions in water, such as chloride ions and bromide ions, resulting in a decrease in removal rate.

[0004] Therefore, it is of great significance to develop a new device for controlling the total DNA concentration in the effluent of sewage treatment plants with less secondary pollution, high drug utilization rate and small anion influence. Utility Model Content

[0005] In response to the above-mentioned defects of the prior art, the utility model provides a batch reaction device for removing total DNA from sewage based on a ferrate and calcium sulfite coupling system, which has the advantages of improving drug utilization, reducing secondary pollution, and resisting water quality influences.

[0006] The utility model adopts the following technical solutions:

[0007] A sequencing batch reaction device for removing total DNA from wastewater based on a ferrate and calcium sulfite coupling system is cylindrical and divided into three partitions, namely, an active species generation zone, a reaction zone, and a precipitation zone from top to bottom. The active species generation zone and the reaction zone, as well as the reaction zone and the precipitation zone are separated by partitions.

[0008] Preferably, the volume ratio of the active species generation zone, the reaction zone and the precipitation zone is 1:2:2.

[0009] Preferably, the shell and the partition of the reaction device are both made of polyvinyl chloride organic glass.

[0010] A potassium ferrate inlet is provided at the top of the side wall of the active species generation zone, and the potassium ferrate inlet is connected to the potassium ferrate dosing device outside the reaction device through a hose; a calcium sulfite inlet is provided at the top of the side wall of the active species generation zone and at a position opposite to the potassium ferrate inlet, and the calcium sulfite inlet is connected to the calcium sulfite dosing device outside the reaction device through a hose; a tap water inlet is provided on the top plate of the active species generation zone; and a stirring paddle is provided at the center of the top plate.

[0011] Preferably, the hose is made of polyurethane.

[0012] A reaction liquid outlet is provided at the bottom of the side wall of the active species generation zone, and a reaction liquid inlet is provided below the reaction liquid outlet and at the top of the side wall of the reaction zone. The reaction liquid outlet and the reaction liquid inlet are connected by a hose, and a metering pump I is provided in the middle of the hose between the two.

[0013] Preferably, the hose is made of EPDM rubber.

[0014] A sewage inlet, an acidic agent inlet, and an alkaline agent inlet are sequentially arranged on the side wall of the reaction zone from top to bottom; the sewage inlet is connected to a metering pump II outside the reaction device; the acidic agent inlet is connected to an acidic agent dosing device outside the reaction device via a hose, and the alkaline agent inlet is connected to an alkaline agent dosing device outside the reaction device via a hose; an acid-base sensing probe is provided in the reaction zone, and the acid-base sensing probe is connected to a pH display outside the reaction device; a submersible agitator is fixedly installed on the bottom partition of the reaction zone, and the submersible agitator is connected to a frequency conversion controller outside the reaction device.

[0015] Preferably, the hose is made of polyurethane.

[0016] A liquid medicine outlet is provided at the bottom of the side wall of the reaction zone, and a liquid medicine inlet is provided below the liquid medicine outlet and at the top of the side wall of the precipitation zone. The liquid medicine outlet and the liquid medicine inlet are connected by a hose, and a metering pump III is provided in the middle of the hose between the two.

[0017] Preferably, the hose is made of EPDM rubber.

[0018] The interior of the sedimentation zone is funnel-shaped, with a purified wastewater discharge port on the upper part of the side wall of the sedimentation zone, which is connected to the metering pump IV outside the reaction device, and a sediment dumping door is provided at the bottom center of the sedimentation zone.

[0019] The beneficial effects of the utility model are:

[0020] 1. In the active species production zone, potassium ferrate and calcium sulfite react fully. Compared to the hydrolysis of potassium ferrate alone in water, calcium sulfite plays a key role in this system, reacting with hexavalent iron to produce more tetravalent and pentavalent iron. Calcium sulfite is also slightly soluble and can continuously react with potassium ferrate to produce tetravalent and pentavalent iron, resulting in sustained-release of the drug. Tetravalent and pentavalent iron have stronger oxidizing properties, are selective, are less affected by anions in the water, and are less susceptible to secondary pollution. Once water containing high-valent iron enters the reaction zone, it can effectively remove DNA from the water, resisting the effects of anions in the water, producing fewer byproducts, and continuously decomposing DNA over a period of time, resulting in significant environmental and social benefits.

[0021] 2. The device of the utility model is divided into three zones: an active species generation zone, a reaction zone, and a precipitation zone. Potassium ferrate has a strong oxidizing ability and can oxidize other components in sewage. By setting up an active species reaction zone, it is ensured that potassium ferrate and calcium sulfite are fully mixed to produce effective ingredients such as tetravalent iron and pentavalent iron, thereby improving the utilization efficiency of the agent and reducing the waste of the agent under the action of impurities in the water. In the reaction zone, the agent and sewage are fully mixed by stirring for a long time, thereby removing pollutants. In the precipitation zone, the iron-containing oxides and hydroxides generated in situ by ferrite can act as coagulants to further reduce the DNA concentration in the water. Through gravity sedimentation, the iron flocculent complex containing DNA is settled at the bottom of the funnel-shaped structure, and the purified wastewater flows out from the discharge port, further improving the purification efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a schematic structural diagram of a batch reaction device for removing total DNA from sewage based on a ferrate and calcium sulfite coupling system according to the present invention.

[0023] In the figure: 1. Active species generation zone; 2. Reaction zone; 3. Precipitation zone; 4. Potassium ferrate dosing device; 5. Calcium sulfite dosing device; 6. Potassium ferrate inlet; 7. Calcium sulfite inlet; 8. Tap water inlet; 9. Stirring paddle; 10. Reaction liquid outlet; 11. Metering pump I; 12. Reaction liquid inlet; 13. Sewage inlet; 14. Metering pump II; 15. Submersible agitator; 16. Frequency conversion controller; 17. Liquid outlet; 18. Metering pump III; 19. Liquid inlet; 20. Acid-base sensing probe; 21. pH display; 22. Alkaline agent dosing device; 23. Acidic agent dosing device; 24. Alkaline agent inlet; 25. Acidic agent inlet; 26. Purified wastewater discharge outlet; 27. Metering pump IV; 28. Sediment dumping gate. DETAILED DESCRIPTION

[0024] Now combined Figure 1 The utility model is further described with specific implementation methods.

[0025] refer to Figure 1 The figure shows a batch reactor for removing total DNA from wastewater based on a ferrate and calcium sulfite coupling system. The reactor is cylindrical and divided into three zones: active species generation zone 1, reaction zone 2, and precipitation zone 3, with a volume ratio of 1:2:2. Partitions separate the active species generation zone 1 from the reaction zone 2, and the reaction zone 2 from the precipitation zone 3. The reactor housing and partitions are made of polyvinyl chloride (PVC) glass, a material with numerous advantages, including chemical resistance, high mechanical strength, leak resistance, cost-effectiveness, ease of processing, durability, fire resistance, environmental friendliness, and lightweight ease of installation.

[0026] A potassium ferrate inlet 6 is provided at the top of the side wall of active species generation zone 1. This inlet 6 is connected to a potassium ferrate dosing device 4 outside the reaction unit via a flexible hose. Potassium ferrate solution flows from the potassium ferrate dosing device 4 and enters the active species generation zone 1 through the potassium ferrate inlet 6. A calcium sulfite inlet 7 is provided at the top of the side wall of active species generation zone 1, opposite the potassium ferrate inlet 6. This inlet 7 is connected to a calcium sulfite dosing device 5 outside the reaction unit via a flexible hose. Calcium sulfite solution flows from the calcium sulfite dosing device 5 and enters the active species generation zone 1 through the calcium sulfite inlet 7. A tap water inlet 8 is provided on the top plate of active species generation zone 1. A speed-controllable stirring paddle 9 is provided at the center of the top plate.

[0027] A reaction liquid outlet 10 is provided at the bottom of the side wall of the active species generation area 1, and a reaction liquid inlet 12 is provided below the reaction liquid outlet 10 and at the top of the side wall of the reaction area 2. The reaction liquid outlet 10 and the reaction liquid inlet 12 are connected by a hose, and a metering pump I11 is provided in the middle of the hose between the two. The metering pump I11 can input the reaction liquid in the active substance generation area 1 from the active substance generation area 1 to the reaction area 2; the hose is made of ethylene propylene rubber, has excellent weather resistance and antioxidant properties, and can be exposed to sunlight, wind, rain and oxygen for a long time without significant aging.

[0028] The sidewalls of reaction zone 2 are provided with a wastewater inlet 13, an acidic agent inlet 25, and an alkaline agent inlet 24, arranged in order from top to bottom. Wastewater inlet 13 is connected to a metering pump II 14 located outside the reaction unit, through which wastewater is pumped through the wastewater inlet 13 and into reaction zone 2. The acidic agent inlet 25 is connected to an acidic agent dosing device 23 located outside the reaction unit via a flexible hose, while the alkaline agent inlet 24 is connected to an alkaline agent dosing device 22 located outside the reaction unit via a flexible hose. The flexible hose is made of polyurethane, which has good tolerance to many chemicals (including acids and bases) and excellent wear resistance. A pH sensor probe 20 is provided within reaction zone 2 and is connected to a pH display 21 located outside the reaction unit. The pH value of the solution in reaction zone 2, detected by the probe 20, is displayed in real time on the pH display 21. The acidic agent dosing device 23 or the alkaline agent dosing device 22 is controlled to replenish the acidic agent or alkaline agent to maintain the pH of the solution in the reaction zone 2. A submersible agitator 15 is fixedly installed on the bottom partition of the reaction zone 2. The submersible agitator 15 is connected to a frequency conversion controller 16 outside the reaction device, and the speed of the submersible agitator 15 is controlled by the frequency conversion controller 16. The submersible agitator 15 is suitable for large-volume stirring and has excellent stability and reliability, suitable for long-term continuous operation. The frequency conversion controller 16 adapted to the submersible agitator 15 can accurately control the speed of the submersible agitator 15 by adjusting the power supply frequency of the motor to meet the needs of different process steps.

[0029] A drug liquid outlet 17 is provided at the bottom of the side wall of the reaction zone 2, and a drug liquid inlet 19 is provided below the drug liquid outlet 17 and at the top of the side wall of the precipitation zone 3. The drug liquid outlet 17 and the drug liquid inlet 19 are connected by a hose, and a metering pump III 18 is provided in the middle of the hose between the two. The metering pump III 18 can input the drug liquid in the reaction zone 2 from the reaction zone 2 to the precipitation zone 3; the hose is also made of EPDM rubber.

[0030] The interior of sedimentation zone 3 is funnel-shaped, with a purified wastewater outlet 26 located at the upper portion of its sidewall. This outlet is connected to a metering pump IV 27 located outside the reactor. A sediment dump gate 28 is located at the bottom center of sedimentation zone 3, i.e., at the bottom of the funnel-shaped structure. Purified wastewater flows out of the purified wastewater outlet 26 via metering pump IV 27, while iron flocculants settle by gravity at the bottom of the funnel-shaped structure. After purification, the wastewater is discharged from the reactor through the sediment dump gate 28.

[0031] The potassium ferrate dosing device 4 and the calcium sulfite dosing device 5 can be used to mix the powdered granular agent with water within the dosing device to prepare a liquid drug of varying concentrations, and then deliver the liquid drug drug to the active species generation zone 1. Potassium ferrate is a strong oxidizing agent and exists in the form of powdered granules. When potassium ferrate is dissolved in water, it will be reduced over time, losing its efficacy.

[0032] The metering pump can automatically and simultaneously complete the functions of conveying, metering and regulating.

[0033] The batch reaction device for removing total DNA from wastewater based on the ferrate and calcium sulfite coupling system provided in this embodiment is as follows: in actual application, analytically pure calcium sulfite powder particles are loaded into the calcium sulfite dosing device 5, and the addition amount of the calcium sulfite dosing device 5 is set to 45.6 mg / L of calcium sulfite (in terms of S). The calcium sulfite solution from the calcium sulfite dosing device 5 enters the active species generation zone 1 through the calcium sulfite inlet 7; analytically pure calcium sulfite powder particles are loaded into the potassium ferrate dosing device 4. Pure potassium ferrate powder particles are added to a potassium ferrate dosing device 4 at a dosage of 40 mg / L of potassium ferrate (calculated as Fe). The potassium ferrate solution from the potassium ferrate dosing device 4 enters the active species generation zone 1 through the potassium ferrate inlet 6. The inlet volume ratio of potassium ferrate to calcium sulfite is 1:1, and the mixed inlet volume accounts for approximately two-thirds of the active species generation zone 1. The stirring paddle 9 is then activated, with the stirring speed set to 600-800 rpm and the stirring time set to 1-2 minutes. After a 2-minute wait, the reaction liquid in the active species generation zone 1 is pumped into the reaction zone 2 via the reaction liquid outlet 10 using a metering pump I 11.

[0034] In reaction zone 2, metering pump II 14 draws domestic sewage through sewage inlet 13 into reaction zone 2, with the volume of the sewage accounting for approximately one-third of reaction zone 2. Acid-base sensing probe 20 monitors the pH of the sewage entering reaction zone 2 in real time, displaying the pH value on pH display 21. If the pH value of the sewage is outside the range of 6.5-7.5, the acidic agent dosing device 23 (filled with a 3% by mass acetic acid solution) and the alkaline agent dosing device 22 (filled with a 2% by mass sodium bicarbonate solution) are controlled to replenish acidic and alkaline agents through acidic agent inlet 25 and alkaline agent inlet 24, respectively, to maintain a near-neutral environment in the sewage. The reaction liquid from reaction liquid inlet 12 enters reaction zone 2 by about one-third of its volume. The next step is to start submerged agitator 15 (located below the liquid level in reaction zone 2) and set frequency converter 16 to a slow speed of 50-100 rpm. Stir for 15-30 minutes to thoroughly mix with the wastewater and remove total DNA. Metering pump III 18 then pumps the liquid from reaction zone 2 into precipitation zone 3 from liquid outlet 17.

[0035] In the precipitation zone 3, the liquid drug after the reaction in the reaction zone 2 enters the precipitation zone 3 through the liquid drug inlet 19. The liquid drug is allowed to stand in the precipitation zone 3 for 30 minutes, allowing the iron flocs to settle to the bottom of the funnel-shaped structure by gravity. The wastewater, separated from the iron flocs, is then discharged from the reaction device through the purified wastewater outlet 26 via metering pump IV 27. The water output is recorded, and a 100 mL water sample is collected and used together with the pre-reaction water for DNA extraction, thereby determining the total DNA removal efficiency of the reaction device. After purification is complete, the sediment discharge door 28 is opened to discharge the iron flocs from the reaction device.

[0036] In practice, after the purified wastewater and iron flocs are discharged from the reactor, the reactor must be cleaned. First, add tap water to the active species production zone 1 from the tap water inlet 8 at the top of the active species production zone 1, with the inlet volume approximately 4 / 5 of the active species production zone 1. Turn on the agitator 9, set the speed to 800 rpm, and stir for 5 minutes to clean the active species production zone 1. After waiting for 2 minutes, open the reaction liquid outlet 10 and use metering pump I 11 to pressurize the cleaning water into the reaction zone 2. Turn on the submersible agitator 15, set the frequency converter 16 to a slow speed of 50-100 rpm, and stir the cleaning water from the reaction liquid inlet 12 for 10 minutes. Allow it to stand for 2 minutes. Then, using metering pump III 18, pressurize the cleaning water through the chemical liquid outlet 17 into the sedimentation zone 3. After the cleaning water entering from the chemical liquid inlet 19 stands in the sedimentation zone 3 for 30 minutes, open the sediment dump door 28 to discharge the cleaning water from the reactor.

[0037] The present invention is described with reference to the above embodiments only. The structure, location, and connection of each component are subject to change. Based on the technical solution of the present invention, any improvement or equivalent transformation of individual components based on the principles of the present invention shall not be excluded from the scope of protection of the present invention.

Claims

1. A batch reaction device for removing total DNA from sewage based on a ferrate and calcium sulfite coupling system, characterized in that: The reaction device is a cylinder and is divided into three partitions, namely, an active species generation zone (1), a reaction zone (2) and a precipitation zone (3) from top to bottom. The active species generation zone (1) and the reaction zone (2), and the reaction zone (2) and the precipitation zone (3) are separated by partitions. A potassium ferrate inlet (6) is provided at the top of the side wall of the active species generation zone (1). The potassium ferrate inlet (6) is connected to a potassium ferrate dosing device (4) outside the reaction device through a hose. A calcium sulfite inlet (7) is provided at the top of the side wall of the active species generation zone (1) and at a position opposite to the potassium ferrate inlet (6). The calcium sulfite inlet (7) is connected to a calcium sulfite dosing device (5) outside the reaction device through a hose. A tap water inlet (8) is provided on the top plate of the active species generation zone (1). A stirring paddle (9) is provided at the center of the top plate.

2. A batch reaction device for removing total DNA from sewage based on a ferrate and calcium sulfite coupling system according to claim 1, characterized in that: A reaction liquid outlet (10) is provided at the bottom of the side wall of the active species generation zone (1), and a reaction liquid inlet (12) is provided below the reaction liquid outlet (10) and at the top of the side wall of the reaction zone (2). The reaction liquid outlet (10) and the reaction liquid inlet (12) are connected by a hose, and a metering pump I (11) is provided in the middle of the hose between the two.

3. The batch reaction device for removing total DNA from sewage based on a ferrate and calcium sulfite coupling system according to claim 1, characterized in that: A sewage inlet (13), an acidic agent inlet (25), and an alkaline agent inlet (24) are sequentially provided on the side wall of the reaction zone (2) from top to bottom; the sewage inlet (13) is connected to a metering pump II (14) outside the reaction device; the acidic agent inlet (25) is connected to an acidic agent dosing device (23) outside the reaction device through a hose, and the alkaline agent inlet (24) is connected to an alkaline agent dosing device (22) outside the reaction device through a hose; an acid-base sensing probe (20) is provided in the reaction zone (2), and the acid-base sensing probe (20) is connected to a pH display (21) outside the reaction device; a submersible agitator (15) is fixedly installed on the bottom partition of the reaction zone (2), and the submersible agitator (15) is connected to a frequency conversion controller (16) outside the reaction device.

4. The batch reaction device for removing total DNA from sewage based on a ferrate and calcium sulfite coupling system according to claim 1, characterized in that: A liquid medicine outlet (17) is provided at the bottom of the side wall of the reaction zone (2), and a liquid medicine inlet (19) is provided below the liquid medicine outlet (17) and at the top of the side wall of the precipitation zone (3). The liquid medicine outlet (17) and the liquid medicine inlet (19) are connected by a hose, and a metering pump III (18) is provided in the middle of the hose between the two.

5. The batch reaction device for removing total DNA from sewage based on a ferrate and calcium sulfite coupling system according to claim 1, characterized in that: The interior of the sedimentation zone (3) is funnel-shaped. A purified wastewater discharge port (26) is provided at the upper portion of the side wall of the sedimentation zone (3). The purified wastewater discharge port (26) is connected to a metering pump IV (27) outside the reaction device. A sediment dumping door (28) is provided at the bottom center of the sedimentation zone (3).

6. The batch reaction device for removing total DNA from wastewater based on a ferrate and calcium sulfite coupling system according to claim 1, characterized in that: The volume ratio of the active species generation zone (1), the reaction zone (2) and the precipitation zone (3) is 1:2:

2.

7. The batch reaction device for removing total DNA from sewage based on a ferrate and calcium sulfite coupling system according to claim 1, characterized in that: The shell and the partition of the sequencing batch reaction device for removing total DNA in sewage based on the ferrate and calcium sulfite coupling system are both made of polyvinyl chloride organic glass.

8. A batch reaction device for removing total DNA from wastewater based on a ferrate and calcium sulfite coupling system according to claim 1 or 3, characterized in that: The hose is made of polyurethane.

9. A batch reaction device for removing total DNA from sewage based on a ferrate and calcium sulfite coupling system according to claim 2 or 4, characterized in that: The hose is made of ethylene propylene rubber.

Images