Purification equipment for bacteriophage

Abstract

The utility model relates to the technical field of bacteriophage preparation, in particular to bacteriophage purification equipment, which comprises a fermentation tank, a centrifugal machine, a first storage tank, an activated carbon membrane stack, a second storage tank, membrane filtration equipment and a third storage tank, the fermentation tank, the centrifugal machine, the first storage tank, the activated carbon membrane stack, the second storage tank, the membrane filtration equipment and the third storage tank are sequentially connected in series through pipelines; sterile compressed air is selectively introduced into the fermentation tank, the centrifugal machine, the first storage tank, the activated carbon membrane stack, the second storage tank, the membrane filtration equipment and the third storage tank to drive liquid to be emptied; the device can effectively reduce the purification time of bacteriophage fermentation liquor, so that the yield in unit time is higher, the device is suitable for large-scale production of bacteriophages, and meanwhile, the device extracts the bacteriophages in a physical mode, does not need chemical reagents, and is not easy to cause hidden dangers to the safety of a user.

Description

Technical Field

[0001] The utility model relates to the technical field of bacteriophage preparation, in particular to bacteriophage purification equipment. Background Art

[0002] Bacteriophages, viruses that specifically infect bacteria, are considered a potential treatment for drug-resistant bacterial infections due to their unique specificity and ability to replicate. Phage therapy can eliminate pathogenic bacteria without affecting beneficial microorganisms in the human body, reducing the risk of side effects associated with traditional antibiotics.

[0003] However, there are many limitations in the laboratory preparation and purification of phages. For example, the existing PEG sedimentation centrifugation method and the method using chemical reagents such as chloroform obtain phage extracts by separating phage fermentation broth.

[0004] Although existing phage purification methods are suitable for laboratory research, they face problems such as insufficient yield and long purification time in large-scale production. In addition, these methods may introduce harmful substances, posing a safety hazard to users. Utility Model Content

[0005] In view of this, the utility model proposes a phage purification device, which provides a fermentation tank, a centrifuge, a first storage tank, an activated carbon membrane stack, a second storage tank, a membrane filtration device and a third storage tank, and drives the fermentation liquid in each device to be emptied by sterile compressed air. The purification time of the phage fermentation liquid can be effectively reduced, so that the output per unit time is higher, and it is suitable for large-scale production of phages. At the same time, the device extracts phages by physical means, does not require the use of chemical reagents, and is not likely to cause hidden dangers to the safety of users.

[0006] The technical solution of the present utility model is achieved as follows:

[0007] The utility model provides a bacteriophage purification device, including a fermentation tank, a centrifuge, a first storage tank, an activated carbon membrane stack, a second storage tank, a membrane filtration device and a third storage tank, wherein:

[0008] The fermentation tank, the centrifuge, the first storage tank, the activated carbon membrane stack, the second storage tank, the membrane filtration device, and the third storage tank are connected in series in sequence through pipelines;

[0009] Sterile compressed air is selectively introduced into the fermentation tank, the centrifuge, the first storage tank, the activated carbon membrane stack, the second storage tank, the membrane filtration device, and the third storage tank to drive liquid drainage.

[0010] On the basis of the above technical solution, preferably, the fermentation tank, the centrifuge, the first storage tank, the activated carbon membrane stack, the second storage tank, the membrane filtration equipment, and the third storage tank are all provided with a compressed air input port.

[0011] On the basis of the above technical solution, preferably, two membrane filtration devices are connected in series between the second storage tank and the third storage tank through a pipeline.

[0012] On the basis of the above technical solution, preferably, the membrane pore diameter of one of the membrane filtration devices close to the second storage tank is 0.45 um, and the membrane pore diameter of the other membrane filtration device is 0.22 um.

[0013] On the basis of the above technical solution, preferably, the activated carbon membrane stack includes a filter tank and a membrane stack assembly, wherein,

[0014] A liquid inlet is provided on one side of the bottom of the filter tank, and the liquid inlet is connected to the first storage tank through a pipeline;

[0015] A liquid outlet is provided in the middle of the bottom of the filter tank, and one end of the liquid outlet is connected to the second storage tank through a pipeline;

[0016] The membrane stack assembly is detachably arranged in the filter tank and covers the liquid outlet. An inner cavity is arranged on the inner side of the membrane stack assembly, and the inner cavity is communicated with the liquid outlet.

[0017] On the basis of the above technical solution, preferably, the activated carbon membrane stack further includes a filter screen, wherein the filter screen is embedded in the upper end of the liquid outlet.

[0018] On the basis of the above technical solution, preferably, the filter tank includes a base and an open tank, wherein,

[0019] The open tank is inverted and detachably arranged on the top of the base, and the top of the open tank is provided with a pressure gauge installation port and an exhaust valve installation hole;

[0020] The liquid inlet and the liquid outlet are arranged on the base.

[0021] On the basis of the above technical solution, preferably, the membrane stack assembly includes an activated carbon filter plate, a bracket, a pressure plate, a lead screw and a lead screw nut, wherein,

[0022] The activated carbon filter plate is in the shape of a circular ring plate, and a plurality of the activated carbon filter plates are stacked on the top of the base. The stacked activated carbon filter plates form a hollow cylinder, and the hollow portion of the hollow cylinder is the inner cavity.

[0023] The bracket is arranged in the hollow of the hollow cylinder and fixed on the top of the base;

[0024] The pressing plate abuts against the top of the hollow cylinder and is sealed;

[0025] One end of the lead screw is detachably mounted on the bracket, and the other end thereof passes through the pressure plate upwards;

[0026] The lead screw nut is screwed onto the upper end of the lead screw, and the lower end of the lead screw nut is rotatably connected to the top of the pressure plate.

[0027] On the basis of the above technical solution, preferably, the membrane stack assembly further includes a handle, wherein,

[0028] The handle is fixed to the side of the lead screw nut.

[0029] On the basis of the above technical solution, preferably, an observation window is provided on the side of the open can.

[0030] The phage purification device of the utility model has the following beneficial effects compared with the prior art:

[0031] (1) By arranging a fermentation tank, a centrifuge, a first storage tank, an activated carbon membrane stack, a second storage tank, a membrane filtration device and a third storage tank, and driving the fermentation liquid in each device to be emptied by sterile compressed air, the purification time of the phage fermentation liquid can be effectively reduced, so that the output per unit time is higher, and it is suitable for large-scale production of phages. At the same time, the device extracts phages by physical means, does not require the use of chemical reagents, and is not likely to cause hidden dangers to the safety of users.

[0032] (2) By selectively introducing sterile compressed air into the fermenter, centrifuge, first storage tank, activated carbon membrane stack, second storage tank, membrane filtration equipment, and third storage tank, it is convenient to inject sterile compressed air of appropriate pressure into each device, so that the fermentation liquid can flow smoothly. At the same time, it can protect the activated carbon filter plate from being damaged by high pressure, making it difficult for activated carbon particles to precipitate on the activated carbon filter plate, which is beneficial to the anti-blocking protection of the subsequent membrane filtration equipment.

[0033] (3) By setting up an activated carbon membrane stack, it is easier to adsorb pigments in the fermentation liquid, making the fermentation liquid clearer.

[0034] (4) Two membrane filtration devices are connected in series through a pipeline between the second storage tank and the third storage tank, and the membrane pore diameter of one membrane filtration device close to the second storage tank is 0.45 μm, and the membrane pore diameter of the other membrane filtration device is 0.22 μm, which facilitates double filtration of impurities in the fermentation liquid, making the liquid clearer.

[0035] (5) By setting a pressure plate to abut against the top of the hollow cylinder and sealing the connection, the contact area between the pressure plate and the activated carbon filter plate is increased. When the pressure plate is used to press down several activated carbon filter plates, the activated carbon filter plates are not easily damaged. At the same time, through the compression of the pressure plate, it is not easy for gaps to appear between two adjacent activated carbon filter plates, and the upper end of the inner cavity can be closed, so that the fermentation liquid in the tank body can only enter the inner cavity after passing through the activated carbon filter plate, and the filtering effect is better. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0037] Figure 1 A plan view of a bacteriophage purification device according to the present invention;

[0038] Figure 2 This is a side view of the activated carbon membrane stack of the present utility model;

[0039] Figure 3 This is a schematic diagram of the internal structure of the activated carbon membrane stack of the present utility model;

[0040] Figure 4 A three-dimensional diagram of a hollow cylinder of the present utility model;

[0041] In the figure: 1. Fermentation tank; 2. Centrifuge; 3. First storage tank; 4. Activated carbon membrane stack; 5. Second storage tank; 6. Membrane filtration equipment; 7. Third storage tank; 41. Filter tank; 42. Membrane stack assembly; 43. Filter screen; 411. Base; 412. Open tank; 421. Activated carbon filter plate; 422. Bracket; 423. Pressing plate; 424. Screw; 425. Screw nut; 426. Handle; 427. Hollow cylinder; 4101. Liquid inlet; 4102. Liquid outlet; 4121. Observation window; 4201. Inner cavity; 41201. Pressure gauge mounting port; 41202. Exhaust valve mounting hole. DETAILED DESCRIPTION

[0042] The following will be combined with the specific embodiments of the present invention to clearly and completely describe the technical solutions of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0043] like Figure 1-4 As shown, a bacteriophage purification device of the present invention includes a fermentation tank 1, a centrifuge 2, a first storage tank 3, an activated carbon membrane stack 4, a second storage tank 5, a membrane filtration device 6 and a third storage tank 7.

[0044] Among them, Figure 1 As shown, the fermentation tank 1, the centrifuge 2, the first storage tank 3, the activated carbon membrane stack 4, the second storage tank 5, the membrane filtration device 6, and the third storage tank 7 are connected in series in sequence through pipelines. Specifically, each device is provided with a feed port and a discharge port, and the feed port and the discharge port are provided with solenoid valves. Among them, the discharge port of the fermentation tank 1 is connected to the feed port of the centrifuge 2 through a pipeline, the discharge port of the centrifuge 2 is connected to the feed port of the first storage tank 3 through a pipeline, the discharge port of the first storage tank 3 is connected to the feed port of the activated carbon membrane stack 4 through a pipeline, the discharge port of the activated carbon membrane stack 4 is connected to the feed port of the second storage tank 5 through a pipeline, the discharge port of the second storage tank 5 is connected to the feed port of the membrane filtration device 6 through a pipeline, the discharge port of the membrane filtration device 6 is connected to the feed port of the membrane filtration device 6 through a pipeline, the discharge port of the membrane filtration device 6 is connected to the feed port of the third storage tank 7 through a pipeline, the discharge port of the third storage tank 7 is used to connect to the filling equipment, and the filtered liquid can also be injected into a disposable sterile bag through the discharge port of the third storage tank 7.

[0045] In the above-mentioned equipment, the flow of the liquid is a single-step flow, that is, sterile compressed air is selectively introduced into the fermenter 1, the centrifuge 2, the first storage tank 3, the activated carbon membrane stack 4, the second storage tank 5, the membrane filtration equipment 6, and the third storage tank 7 to drive the liquid to be emptied. After the sterile compressed air is injected into the fermenter 1, the fermentation liquid flows into the centrifuge 2. When the sterile compressed air is injected into the centrifuge 2, the fermentation liquid flows into the first storage tank 3, and so on. The filtered liquid is finally discharged from the discharge port of the third storage tank 7.

[0046] To achieve the input of sterile compressed air, such as Figure 1 As shown, the fermentation tank 1, the centrifuge 2, the first storage tank 3, the activated carbon membrane stack 4, the second storage tank 5, the membrane filtration device 6, and the third storage tank 7 are all provided with compressed air input ports, wherein each compressed air input port is provided with a solenoid valve.

[0047] During the purification process, the function of fermenter 1 is to culture the host bacteria and then produce phage fermentation liquid.

[0048] The function of the centrifuge 2 is centrifugation. It is a disc centrifuge. Through the centrifugal action of the centrifuge 2, the incompletely lysed cells are broken and the phages are better released. The macromolecules and most cell fragments can be centrifuged out, so that the flux through the membrane in the next step is larger.

[0049] The function of the first storage tank 3 is to store the liquid centrifuged by the centrifuge 2 .

[0050] The function of the activated carbon membrane stack 4 is adsorption filtration. The filtered liquid enters the second storage tank 5 for storage and standby. The pressure resistance of the activated carbon membrane stack 4 is 0.1-0.2 MPa. Through the adsorption filtration of the activated carbon membrane stack 4, the pigment in the liquid is removed, making the liquid clearer.

[0051] The membrane filtration device 6 is any tubular membrane filter in the prior art, such as an internal pressure tubular microfiltration membrane disclosed in CN207025103U, which is used to filter, for example Figure 1 As shown, two membrane filtration devices 6 are connected in series through pipelines between the second storage tank 5 and the third storage tank 7, wherein the membrane pore diameter of one of the membrane filtration devices 6 close to the second storage tank 5 is 0.45um, and the membrane pore diameter of the other membrane filtration device 6 is 0.22um, that is, the pore diameter of the membrane filter element of one membrane filtration device 6 is 0.45um, and the pore diameter of the other is 0.22um. The 0.45um membrane filter element is used for pre-filtration to remove impurities in the fermentation liquid and increase the filtration capacity of the 0.22um membrane filter element. The main purpose of the 0.22um microporous filter element is to further filter and make the liquid cleaner.

[0052] In the phage purification device, the activated carbon membrane stack 4 includes a filter tank 41 and a membrane stack assembly 42 .

[0053] Among them, Figure 2-3 As shown, a liquid inlet 4101 is provided on one side of the bottom of the filter tank 41, and the liquid inlet 4101 is connected to the first storage tank 3 through a pipe. The liquid inlet 4101 is the feed inlet described above; a liquid outlet 4102 is provided in the middle of the bottom of the filter tank 41, and one end of the liquid outlet 4102 is connected to the second storage tank 5 through a pipe. The liquid outlet 4102 is the discharge inlet described above.

[0054] The function of the membrane stack assembly 42 is to filter the solution. It is detachably arranged in the filter tank 41 and covers the liquid outlet 4102. An inner cavity 4201 is arranged on the inner side of the membrane stack assembly 42, and the inner cavity 4201 is connected with the liquid outlet 4102; when the liquid enters the filter tank 41 from the liquid inlet 4101, it passes through the membrane stack assembly 42 and enters the liquid outlet 4102. During the process, filtration is achieved through the membrane stack assembly 42.

[0055] During the filtration process, there may be impurities in the inner cavity 4201. To ensure the cleanliness of the liquid entering the liquid outlet 4102, Figure 3 As shown, a filter screen 43 is embedded at the upper end of the liquid outlet 4102 .

[0056] In the above structure, the filter tank 41 includes a base 411 and an open tank 412 .

[0057] Among them, the open tank 412 is inverted and detachably arranged on the top of the base 411, and the liquid inlet 4101 and the liquid outlet 4102 are arranged on the base 411. Specifically, a sealing ring is arranged between the lower end of the open tank 412 and the top of the base 411, and the side of the lower end of the open tank 412 and the side of the upper end of the base 411 are connected by bolts. The liquid inlet 4101 and the liquid outlet 4102 both extend outward in a pipe shape, and a solenoid valve is arranged on the pipe-like structure to control the on and off of the pipe.

[0058] A pressure gauge mounting port 41201 and an exhaust valve mounting hole 41202 are provided on the top of the open tank 412 for installing a pressure gauge and an automatic exhaust valve. When the liquid in the filter tank 41 needs to be discharged, sterile compressed air is delivered to the filter tank 41. When the pressure reaches 0.1-0.2 MPa, the solenoid valve on the liquid outlet 4102 is opened, and the liquid flows from the liquid outlet 4102 into the second storage tank 5.

[0059] In addition, in order to facilitate external observation of the filtering situation, an observation window 4121 is provided on the side of the open tank 412.

[0060] like Figure 3 As shown, the membrane stack assembly 42 includes an activated carbon filter plate 421, a bracket 422, a pressure plate 423, a screw 424, a screw nut 425 and a handle 426. This structure facilitates the installation and disassembly of the activated carbon filter plate 421 and is not easily damaged during installation.

[0061] The activated carbon filter plate 421 is in the shape of a circular plate, and its main body is made of activated carbon material, such as Figure 3 As shown, there are several activated carbon filter plates 421 stacked on top of the base 411, combined with Figure 4 A plurality of activated carbon filter plates 421 are stacked to form a hollow cylinder 427 , and the hollow portion of the hollow cylinder 427 is an inner cavity 4201 , which is used to store the filtered liquid.

[0062] The function of the bracket 422 is to support the lead screw 424 . The bracket 422 is a triangular support structure, which is arranged in the hollow of the hollow cylinder 427 and fixed to the top of the base 411 .

[0063] The function of the pressure plate 423 is to press the activated carbon filter plate 421. Specifically, one end of the screw 424 is detachably arranged on the bracket 422 by a pin connection, and the other end passes through the pressure plate 423 upward; the screw nut 425 is screwed to the upper end of the screw 424, and the lower end of the screw nut 425 is rotatably connected to the top of the pressure plate 423. When the screw nut 425 is rotated, the screw nut 425 drives the pressure plate 423 to move downward, so that the pressure plate 423 presses downward against the top of the uppermost activated carbon filter plate 421 and continues to rotate. After the screw nut 425 is tightened, several layers of activated carbon filter plates 421 are pressed against the hollow cylinder 427 through the pressing plate 423. After pressing, the pressing plate 423 is held against the top of the hollow cylinder 427. At the same time, the pressing plate 423 seals the upper end of the inner cavity 4201, so that the inner cavity 4201 is not connected to the interior of the open tank 412. The sealed inner cavity 4201 is not in direct contact with the interior of the open tank 412. Therefore, the fermentation liquid in the tank body can only enter the inner cavity 4201 after passing through the activated carbon filter plate 421, and the filtration effect is better.

[0064] like Figure 3 As shown, in order to improve the sealing performance, a rubber pad is set between the pressure plate 423 and the hollow cylinder 427. After tightening the screw nut 425, the rubber pad is clamped and fixed between the pressure plate 423 and the hollow cylinder 427.

[0065] The handle 426 is fixed to the side of the screw nut 425 and its function is to facilitate the screwing of the screw nut 425.

[0066] When the activated carbon filter plate 421 needs to be replaced or installed, the screw nut 425 and the pressure plate 423 are removed from the screw 424, and then the activated carbon filter plate 421 is put on the outside of the screw 424. After multiple layers are stacked, the screw nut 425 and the pressure plate 423 are installed and tightened.

[0067] The method of using the bacteriophage purification device of the utility model is as follows:

[0068] Before using the equipment, open the solenoid valves on the feed and discharge ports of each device, pump clean high-temperature steam into the feed port of the fermenter 1, and the high-temperature steam enters the centrifuge 2, the first storage tank 3, the activated carbon membrane stack 4, the second storage tank 5, the membrane filtration device 6 and the third storage tank 7 in sequence for high-temperature sterilization. The sterilization condition is 0.15MPa (about 123℃) / 15min. After sterilization, inject sterile compressed air to cool down the equipment. After cooling, add phage fermentation material into the fermenter 1.

[0069] The phage material is fermented into phage fermentation liquid in the fermentation tank 1. After the fermentation is completed, sterile compressed air is input into the fermentation tank 1 to press the fermentation liquid into the centrifuge 2. Then, the input of sterile compressed air into the fermentation tank 1 is stopped, and the centrifuge 2 centrifuges the fermentation liquid entering it. After centrifugation, compressed air is input into the centrifuge 2 to press the centrifuged fermentation liquid into the first storage tank 3. Then, the input of sterile compressed air into the centrifuge 2 is stopped, and so on, until the fermentation liquid reaches the discharge port of the third storage tank 7. During the process, the activated carbon membrane stack 4 filters the fermentation liquid to remove impurities and pigments, and the membrane filtration equipment 6 filters the fermentation liquid again to further remove impurities in the liquid. When the sterile compressed air is delivered, the feed port on the corresponding equipment is closed to prevent the liquid from flowing back.

[0070] Since sterile compressed air of appropriate pressure is injected into each device separately, it is convenient to realize separate pressure supply of each device, which is beneficial to the smooth flow of fermentation liquid. At the same time, it can protect the activated carbon filter plate 421 from being damaged by high pressure, making it difficult for activated carbon particles to precipitate on the activated carbon filter plate 421, which is beneficial to the anti-blocking protection of the subsequent membrane filtration device 6.

[0071] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A bacteriophage purification device, comprising a fermentation tank (1), characterized in that: It also includes a centrifuge (2), a first storage tank (3), an activated carbon membrane stack (4), a second storage tank (5), a membrane filtration device (6) and a third storage tank (7), wherein: The fermentation tank (1), the centrifuge (2), the first storage tank (3), the activated carbon membrane stack (4), the second storage tank (5), the membrane filtration device (6), and the third storage tank (7) are sequentially connected in series through pipelines; Sterile compressed air is selectively introduced into the fermentation tank (1), the centrifuge (2), the first storage tank (3), the activated carbon membrane stack (4), the second storage tank (5), the membrane filtration device (6), and the third storage tank (7) to drive liquid discharge.

2. The bacteriophage purification device according to claim 1, characterized in that: The fermentation tank (1), the centrifuge (2), the first storage tank (3), the activated carbon membrane stack (4), the second storage tank (5), the membrane filtration device (6), and the third storage tank (7) are all provided with compressed air input ports.

3. The phage purification device according to claim 1, characterized in that: Two membrane filtration devices (6) are connected in series between the second storage tank (5) and the third storage tank (7) via a pipeline.

4. The phage purification device according to claim 3, characterized in that: The membrane pore diameter of one of the membrane filtration devices (6) close to the second storage tank (5) is 0.45 μm, and the membrane pore diameter of the other membrane filtration device (6) is 0.22 μm.

5. The phage purification device according to claim 1, characterized in that: The activated carbon membrane stack (4) comprises a filter tank (41) and a membrane stack assembly (42), wherein: A liquid inlet (4101) is provided on one side of the bottom of the filter tank (41), and the liquid inlet (4101) is connected to the first storage tank (3) through a pipeline; A liquid outlet (4102) is provided in the middle of the bottom of the filter tank (41), and one end of the liquid outlet (4102) is connected to the second storage tank (5) through a pipeline; The membrane stack assembly (42) is detachably arranged in the filter tank (41) and covers the liquid outlet (4102). An inner cavity (4201) is provided on the inner side of the membrane stack assembly (42), and the inner cavity (4201) is communicated with the liquid outlet (4102).

6. The phage purification device according to claim 5, characterized in that: The activated carbon membrane stack (4) further includes a filter screen (43), wherein the filter screen (43) is embedded in the upper end of the liquid outlet (4102).

7. The phage purification device according to claim 6, characterized in that: The filter tank (41) includes a base (411) and an open tank (412), wherein: The open tank (412) is inverted and detachably arranged on the top of the base (411), and the top of the open tank (412) is provided with a pressure gauge installation port (41201) and an exhaust valve installation hole (41202); The liquid inlet (4101) and the liquid outlet (4102) are arranged on the base (411).

8. The phage purification device according to claim 7, characterized in that: The membrane stack assembly (42) includes an activated carbon filter plate (421), a bracket (422), a pressing plate (423), a lead screw (424) and a lead screw nut (425), wherein: The activated carbon filter plate (421) is in the shape of a circular ring plate. A plurality of the activated carbon filter plates (421) are stacked on top of the base (411). The stacked activated carbon filter plates (421) form a hollow cylinder (427). The hollow portion of the hollow cylinder (427) serves as the inner cavity (4201). The bracket (422) is arranged in the hollow of the hollow cylinder (427) and fixed on the top of the base (411); The pressing plate (423) is pressed against the top of the hollow cylinder (427) and is sealed; One end of the lead screw (424) is detachably arranged on the bracket (422), and the other end thereof passes through the pressing plate (423) upwards; The lead screw nut (425) is screwed onto the upper end of the lead screw (424), and the lower end of the lead screw nut (425) is rotatably connected to the top of the pressure plate (423) via a bearing.

9. The phage purification device according to claim 8, characterized in that: The membrane stack assembly (42) further includes a handle (426), wherein: The handle (426) is fixed to the side of the screw nut (425).

10. The phage purification device according to claim 7, characterized in that: An observation window (4121) is provided on the side of the open tank (412).

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