Automatic coating sterile operation device

Abstract

The utility model belongs to the technical field of microorganisms, and relates to an automatic coating sterile operation device which can be suitable for culture media with different heights and culture dishes with different sizes and is used for uniformly coating bacterial liquid on the surfaces of the culture media. Comprising a base provided with a fixing groove, a telescopic fixing mechanism, a driving motor, a coating mechanism detachably connected to the lower portion of the driving motor, an air blower and an ultraviolet lamp, and the coating mechanism comprises a coating rod, a sterile cover and a culture dish; a contact head at one end of the coating rod is used for coating bacterial liquid at the bottom of the culture dish after the sterile cover and the culture dish are covered, an air blower is used for drying the culture dish before coating, and an ultraviolet lamp can be used for disinfecting the culture dish and the coating rod; automatic sterile coating operation can be carried out on the whole through a simple structure, the whole equipment is convenient to move, transport and clean through a detachable structure, the production and installation cost is reduced, and efficient, uniform and sterile coating operation is achieved.

Description

Technical field:

[0001] The utility model belongs to the field of microbial technology and relates to an automatic aseptic coating operation device, which is used for uniformly coating bacterial liquid on the surface of a culture medium. The device can be applied to culture media of different heights and culture dishes of different sizes, and simplifies the overall operation process. Background technology:

[0002] In the fields of microbiology experiments, biomedical research, and clinical testing, bacterial spreading and inoculation is a basic experimental operation used to evenly distribute the bacterial solution on the surface of the culture medium to facilitate subsequent colony counting, separation, and identification. The traditional manual spreading method is not only cumbersome and time-consuming, but also difficult to ensure the uniformity and repeatability of the spreading. Especially when a large number of samples need to be processed, these human errors will lead to large fluctuations in the results in subsequent microbial counting and other links, ultimately affecting the experimental results. The efficiency and accuracy of manual spreading greatly limit the progress and quality of the experiment. Sterile operation is a key requirement in the spreading process. Any slight contamination may lead to experimental failure or deviation in results. However, in the manual spreading process, maintaining a relatively sterile environment is a more difficult challenge, especially when the culture dish lid is frequently opened and closed for operation, which is more likely to cause contamination.

[0003] In the prior art, Chinese patent CN109182103A discloses an automatic microbial coating device, including a coating rod, a driving mechanism, a coating rod mounting seat and a culture dish cover. The coating rod mounting seat is arranged in the middle of the culture dish cover and is slidably connected to the culture dish cover. The upper end face of the culture dish cover is provided with an ultraviolet lamp. The culture dish cover is also provided with a bacterial suspension dripping hole. A cavity is provided in the coating rod mounting seat. A through hole connecting the cavity and the lower end face of the culture dish cover is provided at the bottom of the coating rod mounting seat. A connecting block is rotatably connected in the through hole. The connecting block is driven to rotate by the driving mechanism. The connecting block is detachably connected to the coating rod. The coating rod is made of an airbag. A rod head block is vertically fixedly connected to the middle of the coating rod. The rod head block is provided with an air vent connected to the coating rod. The driving mechanism is arranged in the cavity of the coating rod mounting seat, which is used to inflate the coating rod and drive the coating rod to rotate.

[0004] Chinese patent CN217781141U discloses a culture dish coating device, comprising: a first fixing mechanism, a second fixing mechanism and a driving mechanism; the first fixing mechanism is used to fix the culture dish; the second fixing mechanism is used to fix the coating rod, and the coating end of the coating rod is located in the culture dish; the driving mechanism is configured to be able to drive the first fixing mechanism and the second fixing mechanism to perform relative motion. This patent sets a driving mechanism to drive the first fixing mechanism and the second fixing mechanism to perform relative motion, thereby driving the coating rod to perform coating motion in the culture dish and realizing automated coating. The use of an automated coating device instead of traditional manual operation can maintain the uniformity of coating in the same batch, and has high work efficiency, low cost, and can prevent cross contamination.

[0005] Chinese patent CN212476733U discloses a microbial culture dish coating device, including a coating rod, a fixing mechanism for placing the culture dish, and an adjusting mechanism for adjusting the position of the coating rod. The adjusting mechanism is arranged on one side of the fixing mechanism, and the fixed end of the coating rod is connected to a connecting component, and the connecting component is arranged above the adjusting component. The working end of the coating rod extends above the fixing mechanism. A fixing bracket for supporting the coating rod is provided between the adjusting mechanism and the fixing mechanism. By setting the fixing mechanism, the coating rod, and the adjusting mechanism, during the process of coating the culture dish, the culture dish is placed on the fixing mechanism. At this time, the height of the working end of the coating rod can be adjusted according to the liquid level position of the culture dish to ensure uniform coating.

[0006] The coating operation devices involved in the above-mentioned prior art all have the problems of complex structure, high manufacturing cost, and inability to be used for containers of various specifications. After searching and analyzing by the inventors, there is no disclosure in the prior art of a coating operation device that uses fixed grooves with depressions of different sizes to limit the culture dish, uses a telescopic rod to adjust the height of the entire coating rod to maintain a suitable coating position, and uses a simple structure to keep the operating environment relatively sterile, and simplifies other operating steps through the integrated adjustable design of the hair dryer and ultraviolet lamp with the coating mechanism. Therefore, the invention of an automatic coating sterile operation device can improve the shortcomings of the prior art, save manufacturing and use costs, reduce manpower requirements, maintain a relatively sterile environment, simplify operating steps, reduce operating difficulty, increase the success rate of coating operations, and can be applied to coating operations performed under conditions of various specifications. Summary of the invention:

[0007] The purpose of the utility model is to overcome the shortcomings of the prior art. Based on the improvement of the coating operation device, a design is provided to limit the culture dish by using a fixed groove with depressions of different sizes, and to adjust the height of the entire coating rod by using a telescopic rod to maintain a suitable coating position. The operating environment is kept relatively sterile through a simple structure. The other operating steps are simplified by the integrated adjustable design of the hair dryer and ultraviolet lamp with the coating mechanism. The problems of the existing operating device such as complex structure, high production cost and inability to be used for containers of various specifications are solved.

[0008] In order to achieve the above-mentioned purpose, the utility model provides an automatic coating aseptic operation device, including a base, a fixing mechanism, a driving motor, and a coating mechanism. A fixing groove is provided on the base for limiting the position of the culture dish when the culture dish is placed on the entire device; a fixing mechanism is fixedly connected to one side of the base, and the fixing mechanism consists of a telescopic part and a fixed part. The telescopic part is used to adjust the height of the entire fixing mechanism. One end of the fixing part is perpendicular to the telescopic part and is fixed to the upper end of the telescopic part, and is used to fix the motor body of the driving motor. The rotating shaft of the driving motor is vertically downward and detachably connected to the coating mechanism, driving the coating mechanism to perform coating operations.

[0009] Furthermore, the fixing groove is composed of multiple concentric cylindrical grooves of different diameters, the top surface of the cylindrical groove and the top surface of the base are located in the same plane, the smaller the diameter, the deeper the groove, and the maximum depth of the fixing groove does not exceed the height of the culture dish.

[0010] Furthermore, the telescopic component is a telescopic rod, including an adjusting buckle, an outer rod and an inner rod. One end of the inner rod is inserted into the outer rod, and the adjusting buckle is arranged at the connection between the outer rod mouth and the inner rod. The relative position of the inner rod and the outer rod can be fixed by closing the adjusting buckle to clamp the connection between the outer rod mouth and the inner rod.

[0011] Furthermore, the coating mechanism includes a coating rod, a sterile cover and a culture dish. The coating rod is divided into a support rod and a contact head. A contact head is fixed at one end of the support rod. The contact head is used to directly contact the bacterial liquid for coating. The contact head has a bottom surface perpendicular to the support rod so as to increase the area of contact with the bacterial liquid; the sterile cover is a circular cover with a circular hole in the center of the cover surface. The diameter of the circular hole is slightly larger than the bottom surface diameter of the coating rod support rod, so that the coating rod can pass through the circular hole with almost no gap. After the sterile cover and the culture dish are covered, the contact head of the coating rod coats the bacterial liquid on the bottom of the culture dish. The size of the culture dish and the sterile cover are matched so that the edges of the two are consistent, and the sterile cover can just cover the culture dish.

[0012] Furthermore, the lower end of the rotating shaft of the driving motor has an external thread, the upper end of the coating rod support rod is hollow and provided with an internal thread, and the rotating shaft and the coating rod are connected by threads to achieve free disassembly.

[0013] Furthermore, the coating rod is a "T"-shaped rod consisting of a long supporting rod and a short rod contact head perpendicular to the long rod and with its center connected to one end of the long rod.

[0014] Furthermore, a blower is installed downwardly on the fixed component, and the air outlet of the blower is arranged toward the base, so as to dry the culture dish before the coating operation.

[0015] Furthermore, the automatic coating aseptic operation device also includes an ultraviolet lamp, which is movably connected to the telescopic component through an adjustable buckle, and the lamp mouth is set in the direction of the culture dish, which can be used to disinfect the culture dish and the coating rod.

[0016] Furthermore, the side of the culture dish is marked with scale.

[0017] Compared with the prior art, the present invention has the following advantages: (1) The simple structure of concentric cylindrical grooves of different diameters is used to make the base adaptable to culture dishes of various sizes and ensure that the culture dishes are stably fixed during the coating process, avoiding uneven coating caused by position offset, and having adaptability and stability; (2) The height of the entire fixing mechanism can be adjusted by adjusting the telescopic parts to adapt to culture media of different thicknesses and coating requirements. The overall device has strong flexibility and a wide range of applications; (3) The ultraviolet lamp can be used to disinfect the culture dishes, sterile covers and coating rods before coating, reducing the risk of experimental contamination and ensuring the accuracy of experimental results; the hair dryer provided thereon The culture dish can be dried before the coating operation to prevent excessive moisture from affecting the growth of the colony or diluting the concentration of the bacterial solution; the integrated combination of the ultraviolet lamp, the hair dryer and the overall device simplifies the complex steps of the traditional coating operation, reduces the possibility of human error, and improves work efficiency; (4) The setting of the sterile cover can prevent the device from splashing liquid or other contamination during the coating operation, and cooperates with the ultraviolet lamp to ensure that the entire operating environment is relatively sterile; the overall simple structure can perform automated sterile coating operations, and the detachable structure makes the overall equipment easy to move and transport and easy to clean, reducing production and installation costs, and achieving efficient, uniform and sterile coating operations. Description of the drawings:

[0018] Figure 1 This is a schematic diagram of the overall structural principle of the automatic coating aseptic operation device involved in the present utility model.

[0019] Figure 2 The utility model is a schematic diagram of the structural principle of the sterile cover and culture dish.

[0020] Figure 3 This is a schematic side sectional view of the fixing groove involved in the present utility model.

[0021] The components in the accompanying drawings are marked as: base 1, fixing groove 2, adjusting buckle 3, outer rod 4, inner rod 5, fixing component 6, drive motor 7, rotating shaft 8, coating rod 9, support rod 10, contact head 11, sterile cover 12, culture dish 13, circular hole 14, scale 15, hair dryer 16, and ultraviolet lamp 17. Specific implementation method:

[0022] The technical solution of the present utility model is further described below with reference to the accompanying drawings and specific embodiments.

[0023] Example 1:

[0024] The present embodiment relates to an automatic coating aseptic operation device, including a base 1, a fixing mechanism, a drive motor 7, and a coating mechanism. A fixing groove 2 is provided on the base 1 for limiting the position of the culture dish 13 when the culture dish 13 is placed on the entire device; a fixing mechanism is fixedly connected to one side of the base 1, and the fixing mechanism consists of a telescopic part and a fixed part 6. The telescopic part is used to adjust the height of the entire fixing mechanism. One end of the fixed part 6 is perpendicular to the telescopic part and fixed to the upper end of the telescopic part, and is used to fix the motor body of the drive motor 7. The rotating shaft 8 of the drive motor 7 is vertically downward and detachably connected to the coating mechanism, driving the coating mechanism to perform coating operations.

[0025] The fixing groove 2 is composed of multiple concentric cylindrical grooves of different diameters. The top surface of the cylindrical groove is in the same plane as the top surface of the base 1. The smaller the diameter, the deeper the groove. The maximum depth of the fixing groove 2 does not exceed the height of the culture dish 13. The fixing groove 2 involved in this embodiment is composed of three concentric cylindrical grooves. The bottom diameters from small to large are 60mm, 90mm, and 100mm, respectively, and the height is 10mm.

[0026] The telescopic component involved in this embodiment is a telescopic rod, including an adjusting buckle 3, an outer rod 4 and an inner rod 5. One end of the inner rod 5 is inserted into the outer rod 4, and the adjusting buckle 3 is arranged at the connection between the outer rod 4 and the inner rod 5. The relative position of the inner rod 5 and the outer rod 4 can be fixed by closing the adjusting buckle 3 to clamp the connection between the outer rod 4 and the inner rod 5.

[0027] The coating mechanism involved in this embodiment includes a coating rod 9, a sterile cover 12 and a culture dish 13. The coating rod 9 is divided into a support rod 10 and a contact head 11. The contact head 11 is fixed at one end of the support rod 10. The contact head 11 is used to directly contact the bacterial solution for coating. The contact head 11 has a bottom surface perpendicular to the support rod 10 so as to increase the area of contact with the bacterial solution; the sterile cover 12 is a circular cover with a circular hole 14 in the center of the cover surface. The diameter of the circular hole 14 is slightly larger than the bottom diameter of the support rod 10 of the coating rod 9, so that the coating rod 9 can pass through the circular hole 14 with almost no gap. After the sterile cover 12 and the culture dish 13 are covered, the contact head 11 of the coating rod 9 performs a coating operation on the bacterial solution at the bottom of the culture dish 13. The size of the culture dish 13 and the sterile cover 12 are matched so that the edges of the two are consistent, and the sterile cover 12 can just cover the culture dish 13.

[0028] The lower end of the rotating shaft 8 of the driving motor 7 involved in this embodiment has an external thread, and the upper end of the coating rod 9 support rod 10 is hollow and provided with an internal thread. The rotating shaft 8 and the coating rod 9 are connected by threads to achieve free disassembly.

[0029] The coating rod 9 involved in this embodiment is a "T"-shaped rod composed of a long support rod 10 and a short rod contact head 11 perpendicular to the long rod and connected at its center to one end of the long rod, and is made of polypropylene.

[0030] The fixing component 6 involved in this embodiment is provided with a blower 16 mounted downwardly. The air outlet of the blower 16 is arranged toward the base 1 and is used to dry the culture dish 13 before the coating operation.

[0031] The automatic coating aseptic operation device involved in this embodiment also includes an ultraviolet lamp 17, which is movably connected to the telescopic component through an adjustable buckle. The lamp mouth is set in the direction of the culture dish 13 and can be used to disinfect the culture dish 13 and the coating rod 9.

[0032] The culture dish 13 in this embodiment is made of glass, and a scale 15 is marked on the side thereof.

[0033] The culture dish 13, sterile cover 12 and fixing groove 2 involved in this embodiment are all complete sets of components, and the same set of dimensions are matched. There are multiple sets in total, with three sizes of 60 mm, 90 mm and 100 mm.

[0034] The method of using the automatic coating aseptic operation device involved in this embodiment is as follows:

[0035] S1: pre-immerse the coating rod 9, culture dish 13 and sterile cover 12 in a beaker containing 75% alcohol for disinfection;

[0036] S2: Take out the coating rod 9, culture dish 13 and sterile cover 12, and after drying them slightly, insert the culture dish 13 into the fixing groove 2 of the matching size on the base 1, and place the sterile cover 12 with the inner part facing upward on the base 1 of the automatic coating aseptic operation device, and place the coating rod 9 on top of the culture dish 13 or the sterile cover 12;

[0037] S3: Turn on the ultraviolet lamp 17 to sterilize the culture dish 13, the sterile cover 12 and the coating rod 9 with ultraviolet light;

[0038] S4: Pour an appropriate amount of culture medium solution into the culture dish 13, adjust the culture medium height according to the scale 15, and wait for 5-8 minutes for the culture medium to solidify;

[0039] S5: Turn on the blower 16 on the fixed component 6 to keep the coating rod 9, the culture dish 13, the sterile cover 12 and the surface of the culture medium relatively dry to prevent excessive moisture during coating from causing the colonies to spread into pieces or diluting the bacterial solution concentration, thereby affecting the experimental results. After the blowing is completed, turn off the blower 16 and the ultraviolet lamp 17;

[0040] S6: Pipette an appropriate amount of bacterial solution and add it dropwise to the inner surface of the culture dish 13. The general amount of sample is 0.1-0.2 ml.

[0041] S7: Pass the upper end of the support rod 10 of the coating rod 9 through the circular hole 14 of the sterile cover 12 from under the sterile cover 12, and then connect the upper end of the coating rod 9 to the lower end of the rotating shaft 8 of the driving motor 7 through threads. First, adjust the length of the telescopic rod by pulling out or extending the inner rod 5 of the telescopic rod into the outer rod 4, and fix the positional relationship between the outer rod 4 and the inner rod 5 by adjusting the buckle 3. Then, slightly adjust the number of rotations of the threads of the coating rod 9 and the rotating shaft 8, so that the sterile cover 12 can be covered on the culture dish 13 and the coating rod 9 can be just perpendicular to the bottom surface of the culture dish 13 and contact the surface of the culture medium;

[0042] S8: Starting the drive motor 7 to rotate the shaft 8, thereby driving the coating rod 9 connected to the lower end of the shaft 8 to rotate. The contact head 11 of the coating rod 9 can evenly coat the bacterial solution on the surface of the culture medium;

[0043] S9: After the coating is completed, the drive motor 7 is turned off, and the telescopic rod is adjusted so that the coating rod 9 and the sterile cover 12 are separated from the culture dish 13, the culture dish 13 is removed from the fixing groove 2 of the base 1, and the sterile cover 12 and the coating rod 9 are disassembled;

[0044] S10: Cleaning the disassembled sterile cover 12 and coating rod 9.

[0045] Example 2:

[0046] The implementation of this embodiment is to verify the use effect of the automatic coating aseptic operation device involved in Example 1, and mainly carried out the following comparative experiments;

[0047] (1) Experimental equipment:

[0048] Experimental group: using the automatic coating aseptic operation device involved in Example 1;

[0049] Control group: manual operation using existing technology;

[0050] (2) Experimental methods:

[0051] The same volume of the same bacterial solution was coated, and the time, uniformity, and success rate of the coating operation were compared between the experimental group and the control group.

[0052] (3) Experimental results:

[0053] Table 1:

[0054] Operation time Uniformity Success rate Experimental group 2min 100％ 100％ control group 6min 82％ 98％

[0055] It can be seen from Table 1 that the automatic coating aseptic operation device involved in this embodiment is superior to the control group in terms of the time spent, uniformity and success rate during the coating operation. It can perform coating operations on the covered culture dish 13 through the motor shaft 8 and the coating rod 9. High uniformity and success rate can be achieved through mechanized automatic coating. The hair dryer 16 and ultraviolet lamp 17 provided in the device eliminate the need to perform steps such as drying and disinfection separately, which can simplify the preparation process, reduce the chance of contamination, and increase the overall operation success rate. It is suitable for a variety of culture dish 13 size requirements, solves the problems existing in traditional manual coating methods and existing technologies, and provides strong technical support for experiments in related fields such as microbiology.

Claims

1. An automatic coating aseptic operation device, characterized in that: It includes a base, a fixing mechanism, a driving motor, and a coating mechanism. A fixing groove is provided on the base for limiting the position of the culture dish when the culture dish is placed on the entire device; a fixing mechanism is fixedly connected to one side of the base, and the fixing mechanism consists of a telescopic part and a fixed part. The telescopic part is used to adjust the height of the entire fixing mechanism. One end of the fixing part is perpendicular to the telescopic part and fixed to the upper end of the telescopic part, and is used to fix the motor body of the driving motor. The rotating shaft of the driving motor is vertically downward and detachably connected to the coating mechanism, driving the coating mechanism to perform coating operations.

2. The automatic coating aseptic operation device according to claim 1, characterized in that: The fixing groove is composed of multiple concentric cylindrical grooves of different diameters. The top surface of the cylindrical groove is in the same plane as the top surface of the base. The smaller the diameter, the deeper the groove. The maximum depth of the fixing groove does not exceed the height of the culture dish.

3. The automatic coating aseptic operation device according to claim 1, characterized in that: The telescopic component is a telescopic rod, which includes an adjusting buckle, an outer rod and an inner rod. One end of the inner rod is inserted into the outer rod, and the adjusting buckle is arranged at the connection between the outer rod mouth and the inner rod. The connection between the outer rod mouth and the inner rod can be clamped by closing the adjusting buckle, so that the relative position of the inner rod and the outer rod is fixed.

4. The automatic coating aseptic operation device according to claim 1, characterized in that: The coating mechanism includes a coating rod, a sterile cover and a culture dish. The coating rod consists of a support rod and a contact head. The contact head is fixed to one end of the support rod, and the contact head has a bottom surface perpendicular to the support rod. The sterile cover is a circular cover with a circular hole in the center of the cover surface. The diameter of the circular hole is larger than the bottom diameter of the coating rod support rod, so that the coating rod can pass through the circular hole. The size of the culture dish and the sterile cover are matched so that the edges of the two are consistent, and the sterile cover can just cover the culture dish.

5. The automatic coating aseptic operation device according to claim 4, characterized in that: The coating rod is a "T"-shaped rod consisting of a long supporting rod and a short rod contact head perpendicular to the long rod and connected at its center to one end of the long rod.

6. The automatic coating aseptic operation device according to claim 4, characterized in that: The lower end of the rotating shaft of the driving motor has an external thread, the upper end of the coating rod support rod is hollow and provided with an internal thread, and the rotating shaft and the coating rod are connected by threads to achieve free disassembly.

7. The automatic coating aseptic operation device according to claim 1, characterized in that: A hair dryer is installed downwardly on the fixing component, and an air outlet of the hair dryer is arranged toward the base.

8. The automatic coating aseptic operation device according to claim 1, characterized in that: The automatic coating aseptic operation device also includes an ultraviolet lamp, which is movably connected to the telescopic component through an adjustable buckle, and the lamp mouth is arranged in the direction of the coating mechanism.

9. The automatic coating aseptic operation device according to claim 4, characterized in that: The side of the culture dish is marked with scale.

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