Device for measuring activity of phytase in phytase fermentation liquor

Abstract

The utility model belongs to the field of phytase, in particular relates to a device for measuring the activity of phytase in phytase fermentation liquor, and aims to solve the problems that two motors are required to drive a rotary drum to perform separate centrifugation during centrifugal treatment in the prior art, so that integrated centrifugal treatment is inconvenient to realize, and meanwhile, when centrifuged liquid is transferred, the centrifugal liquid is difficult to transfer. In order to solve the problem that the operation convenience is reduced due to the phenomenon that the sample liquid is difficult to extract when the pressure in a centrifugal box is too low due to the fact that the sample liquid needs to be transferred in a piston extraction mode, the following scheme is provided: the device comprises liquid extracting mechanisms, and the liquid extracting mechanisms are arranged on a lower centrifugal box and an upper centrifugal box and are used for receiving the sample liquid in the lower centrifugal box and the upper centrifugal box. When the centrifugal machine is used, the effect of integrated centrifugal machining is achieved, two motors do not need to drive the rotary drums to conduct centrifugal separately, meanwhile, transfer does not need to be conducted in a piston extraction mode, the phenomenon that extraction is difficult when the pressure in the centrifugal box is too low is prevented, and operation convenience is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of phytase, in particular to a device for determining phytase activity in phytase fermentation liquid. Background Art

[0002] Phytase is a general term for a class of enzymes that catalyze the hydrolysis of phytic acid and its salts into inositol and phosphate (salt). It is a phosphate monoester hydrolase with a special spatial structure. When measuring the phytase activity in phytase fermentation broth, a measuring device is required, and the phytase often needs to be processed before the measurement.

[0003] After searching, the patent with authorization announcement number CN210620811U proposes a device for measuring phytase activity in phytase fermentation liquid, including a base, the top of the base is provided with a first centrifugal box, a separation box, a second centrifugal box and a spectrophotometer, the separation box is located between the first centrifugal box and the second centrifugal box, the second centrifugal box is located between the separation box and the spectrophotometer, and the inner bottom of the first centrifugal box is fixedly connected with a first motor by bolts. The device for measuring phytase activity in the phytase fermentation liquid, through the mutual cooperation of the first centrifugal box, the separation box, the second centrifugal box and the spectrophotometer, makes phytase be in a closed environment during the overall extraction process, on the one hand avoids the influence of bacteria, impurities and temperature in the environment on phytase activity, improves the accuracy of phytase activity determination, and on the other hand improves the extraction efficiency of the whole phytase, effectively reducing the time consumed during the transfer process of the extract. There are the following problems in this technical solution:

[0004] In the existing processing of phytase fermentation liquid, two motors are required to drive the drums to centrifuge separately during the centrifugal treatment, which is not convenient for realizing integrated centrifugal treatment, thereby increasing processing costs. In addition, when transferring the centrifuged liquid, piston extraction is required, which makes extraction difficult when the pressure in the centrifuge box is too low, thereby reducing operational convenience.

[0005] In response to the above problems, the present utility model document proposes a device for measuring phytase activity in phytase fermentation broth. Utility Model Content

[0006] The utility model provides a device for determining phytase activity in phytase fermentation broth, which solves the problem in the prior art that, during centrifugal treatment, two motors need to be used to drive the drums to centrifuge separately, which is inconvenient to achieve integrated centrifugal treatment and increases processing costs. At the same time, when transferring the liquid after centrifugation, it is also necessary to transfer it by piston extraction, which makes extraction difficult when the pressure in the centrifuge box is too low, thereby reducing the convenience of operation.

[0007] The utility model provides the following technical solutions:

[0008] A device for measuring phytase activity in phytase fermentation broth, comprising:

[0009] A base, the bottom side of the base is provided with an anti-slip pad, the top side of the base is fixedly provided with two support rods, the top of the two support rods is fixedly provided with a lower centrifugal box, the top side of the lower centrifugal box is symmetrically fixed with connecting blocks, and the top sides of the four connecting blocks are fixedly provided with upper centrifugal boxes;

[0010] The liquid taking mechanism is arranged on the lower centrifugal box and the upper centrifugal box for taking the sample liquid in the lower centrifugal box and the upper centrifugal box.

[0011] In one possible design, a motor is fixedly mounted on the bottom side of the lower centrifugal box, the output end of the motor passes through the bottom side of the lower centrifugal box and extends to the interior of the lower centrifugal box, the output end of the motor is fixedly provided with a drive rod through a coupling, the outer wall of the drive rod is fixedly provided with a lower drum, the top end of the drive rod passes through the top side of the inner wall of the lower centrifugal box and the bottom side of the upper centrifugal box in sequence and extends to the interior of the upper centrifugal box, and the top end of the drive rod is fixedly provided with an upper drum.

[0012] In one possible design, the liquid extraction mechanism includes two discharge pipes, two solenoid valves, two discharge pipes and two valves. The two discharge pipes are respectively connected to the outer walls of the lower rotating drum and the upper rotating drum, each solenoid valve is arranged on the outer wall of the discharge pipe, the two discharge pipes are respectively connected to the outer walls of the lower centrifugal box and the upper centrifugal box, and each valve is arranged on the outer wall of the discharge pipe.

[0013] In a possible design, the outer walls of the lower centrifugal box and the upper centrifugal box are fixedly provided with supporting plates corresponding to the discharge pipe, and the outer walls of the lower centrifugal box and the upper centrifugal box are respectively connected to the feed pipe and the liquid inlet pipe.

[0014] In a possible design, a mounting groove is provided inside the lower centrifugal box, and a plurality of electric heating tubes are fixedly installed inside the mounting groove.

[0015] In a possible design, a spectrophotometer for measuring phytase activity is provided on the top of the base.

[0016] In the present application, when in use, the phytase fermentation liquid is first injected into the upper drum in the upper centrifugal box through the feed pipe, and then the motor installed at the bottom of the lower centrifugal box is turned on to run, and the driving rod fixed at the output end is driven to rotate by the motor, and the driving rod will drive the lower drum and the upper drum fixed on the outer wall and the top to rotate inside the corresponding lower centrifugal box and the upper centrifugal box, and then the high-speed rotating upper drum will remove the bacteria in the phytase fermentation liquid and enhance the quality of the fermentation liquid, while the high-speed rotation of the lower drum facilitates the subsequent further centrifugal processing of the enzyme liquid, thereby achieving the effect of synchronous centrifugation of the two drums and realizing the effect of integrated centrifugal processing, and there is no need to use two motors. The drum is driven to separate and centrifuge, thereby reducing processing costs. After the bacteria in the phytase fermentation liquid are removed, the dialysis bag is placed on the corresponding support plate to match the discharge pipe on the upper centrifuge box. At this time, by controlling the switch state of the solenoid valve, the discharge pipe is selectively opened to allow the clear liquid after centrifugation to flow out of the drum and be discharged through the discharge pipe. The regulating valve on the outer wall of the discharge pipe can be used to control the speed and amount of discharge. Finally, the clear liquid flows into the corresponding dialysis bag to achieve the purpose of seamless liquid connection and avoid contamination by bacterial impurities in the air. The dialysis bag is then placed in external ion water. After two hours, an enzyme solution with inorganic phosphorus removed can be obtained;

[0017] Next, the obtained organophosphorus enzyme liquid is injected into the lower drum in the lower centrifuge box through the liquid inlet pipe, and then the enzyme liquid can be further centrifuged by the high-speed rotation of the lower drum. At the same time, the electric heating tube in the installation groove is turned on to heat the enzyme liquid inside the lower drum to optimize the temperature conditions for enzyme activity determination and improve the determination effect. After a period of time, the color stop solution is added and allowed to stand. Then the liquid collection bottle is placed on the corresponding support plate, which can be used to support and place the liquid, so as to enhance the applicability. Subsequently, the solenoid valve and valve at the position of the lower centrifuge box are opened to discharge the processed liquid into the sampling bottle for later placement and determination. Therefore, when the liquid after centrifugation is transferred, it is no longer necessary to transfer it by piston extraction, so as to prevent the phenomenon of difficulty in extraction when the pressure in the centrifuge box is too low, thereby improving the convenience of operation. Finally, the liquid in the sampling bottle is placed in the spectrophotometer on the top of the base for measurement and analysis, so as to achieve the purpose of phytase activity determination and be easy to use.

[0018] In the utility model, the device for determining phytase activity in phytase fermentation broth can drive the lower drum 11 and the upper drum, whose outer wall and top are fixed, to rotate inside the corresponding lower centrifugal box and upper centrifugal box through the arrangement of the motor, the driving rod, the lower drum and the upper drum. This achieves the effect of synchronous centrifugation of the two drums and realizes the effect of integrated centrifugal processing.

[0019] In the utility model, the device for determining phytase activity in phytase fermentation broth can achieve the purpose of seamless liquid connection through the liquid taking mechanism, thereby avoiding contamination by bacterial impurities in the air, and at the same time, no longer needs to be transferred by piston extraction;

[0020] In the utility model, the effect of integrated centrifugal processing is achieved, and there is no need to use two motors to drive the drum to centrifuge separately, thereby reducing processing costs. At the same time, there is no need to transfer by piston extraction, which prevents the phenomenon of difficulty in extraction when the pressure in the centrifugal box is too low, thereby improving the convenience of operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a schematic diagram of the main structure of a device for measuring phytase activity in phytase fermentation broth provided by an embodiment of the present utility model;

[0022] Figure 2 This is a second schematic diagram of the main structure of a device for measuring phytase activity in a phytase fermentation broth provided by an embodiment of the present utility model;

[0023] Figure 3 A schematic cross-sectional view of a lower centrifuge box and an upper centrifuge box of a device for measuring phytase activity in a phytase fermentation broth provided by an embodiment of the present utility model;

[0024] Figure 4 The present invention provides a device for measuring phytase activity in phytase fermentation liquid. Figure 3 Schematic diagram of the enlarged structure of part A.

[0025] Reference numerals:

[0026] 1. Base; 2. Support rod; 3. Lower centrifuge box; 4. Connecting block; 5. Upper centrifuge box; 6. Feed pipe; 7. Liquid inlet pipe; 8. Support plate; 9. Motor; 10. Drive rod; 11. Lower drum; 12. Upper drum; 13. Discharge pipe; 14. Solenoid valve; 15. Discharge pipe; 16. Valve; 17. Mounting slot; 18. Electric heating tube; 19. Anti-slip mat; 20. Spectrophotometer. DETAILED DESCRIPTION

[0027] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0028] Example 1

[0029] Please refer to Figure 1-Figure 4 , a measuring device comprising:

[0030] The base 1 is provided with an anti-skid pad 19 on the bottom side of the base 1. The setting of the anti-skid pad 19 improves the anti-skid stability of the overall placement of the device. The top side of the base 1 is fixedly provided on two support rods 2. The tops of the two support rods 2 are fixedly provided with lower centrifugal boxes 3. The top sides of the lower centrifugal boxes 3 are symmetrically fixed with connecting blocks 4. The top sides of the four connecting blocks 4 are fixedly provided with upper centrifugal boxes 5. By connecting the lower centrifugal box 3 and the upper centrifugal box 5 through the connecting blocks 4, the overall centrifugal processing can be realized, which improves the convenience.

[0031] The top of the driving rod 10 passes through the top side of the inner wall of the lower centrifuge box 3 and the bottom side of the upper centrifuge box 5 in sequence and extends to the interior of the upper centrifuge box 5. The top of the driving rod 10 is fixedly provided with an upper drum 12. The driving rod 10 fixed at the output end is driven by the motor 9 to rotate, and the driving rod 10 will drive the lower drum 11 and the upper drum 12 fixed on the outer wall and the top to rotate in the corresponding lower centrifuge box 3 and the upper centrifuge box 5. Then, the high-speed rotating upper drum 12 will remove the bacteria in the phytase fermentation liquid and enhance the quality of the fermentation liquid. The high-speed rotation of the lower drum 11 facilitates the subsequent further centrifugal processing of the enzyme liquid, thereby achieving the effect of synchronous centrifugation of the two drums and realizing the effect of integrated centrifugal processing.

[0032] The liquid taking mechanism is arranged on the lower centrifugal box 3 and the upper centrifugal box 5 for collecting the sample liquid in the lower centrifugal box 3 and the upper centrifugal box 5. The liquid taking mechanism includes two discharge pipes 13, two solenoid valves 14, two discharge pipes 15 and two valves 16. The two discharge pipes 13 are respectively connected to the outer walls of the lower rotating drum 11 and the upper rotating drum 12. Each solenoid valve 14 is arranged on the outer wall of the discharge pipe 13. The two discharge pipes 15 are respectively connected to the outer walls of the lower centrifugal box 3 and the upper centrifugal box 5. Each valve 16 is arranged on the outer wall of the discharge pipe 15.

[0033] By controlling the switching state of the solenoid valve 14, the discharge pipe 13 is selectively opened to allow the clear liquid after centrifugation to flow out of the drum and be discharged through the discharge pipe 15. The regulating valve 16 on the outer wall of the discharge pipe 15 can be used to control the speed and amount of discharge, and finally the clear liquid flows into the corresponding dialysis bag. Subsequently, the solenoid valve 14 and valve 16 at the position of the lower centrifuge box 3 are opened to discharge the processed liquid into the sampling bottle for later placement and measurement, thereby achieving the purpose of seamless liquid connection and avoiding contamination by bacterial impurities in the air. When the liquid after centrifugation is received and transferred, it is no longer necessary to transfer it by piston extraction, to prevent the phenomenon of difficulty in extraction when the pressure in the centrifuge box is too low.

[0034] The outer walls of the lower centrifuge box 3 and the upper centrifuge box 5 are fixedly provided with a support plate 8 corresponding to the discharge pipe 15. The outer walls of the lower centrifuge box 3 and the upper centrifuge box 5 are respectively connected with the feed pipe 6 and the liquid inlet pipe 7. The dialysis bag is placed on the corresponding support plate 8 to match the discharge pipe 15 on the upper centrifuge box 5. The liquid collection bottle is placed on the corresponding support plate 8. The support plate 8 can be used to support and place the bag in order to enhance applicability.

[0035] The present application can be used in the field of phytase, and can also be used in other fields applicable to the present application.

[0036] The model of the spectrophotometer 20 is UV-1500, and its structure and mechanism are not described here in detail.

[0037] Example 2

[0038] refer to Figure 1-Figure 3 , based on the improvement of Example 1: a device for measuring phytase activity in phytase fermentation broth, which is applied to the field of phytase;

[0039] An installation groove 17 is provided inside the lower centrifuge box 3, and a plurality of electric heating tubes 18 are fixedly installed inside the installation groove 17. When the electric heating tubes 18 in the installation groove 17 are opened, the enzyme solution inside the lower drum 11 is heated to optimize the temperature conditions for enzyme activity determination and improve the determination effect.

[0040] A spectrophotometer 20 for measuring phytase activity is provided on the top of the base 1 . The liquid in the sampling bottle is placed in the spectrophotometer 20 on the top of the base 1 for measurement and analysis, thereby achieving the purpose of measuring phytase activity.

[0041] However, as is well known to those skilled in the art, the working principles and wiring methods of the motor 9, solenoid valve 14, electric heating tube 18 and spectrophotometer 20 are commonplace, and are conventional means or common knowledge, and will not be elaborated here. Those skilled in the art can make any selections according to their needs or convenience.

[0042] The above are only specific embodiments of the present invention, but the scope of protection of the present invention is not limited to them. Any changes or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in this utility model should be included in the scope of protection of the present utility model. The embodiments of the present utility model and the features of the embodiments can be combined with each other unless there is a conflict. Therefore, the scope of protection of the present utility model shall be based on the scope of protection of the claims.

Claims

1. A device for measuring phytase activity in phytase fermentation broth, characterized in that: include: A base (1), wherein the bottom side of the base (1) is provided with an anti-slip pad (19), the top side of the base (1) is fixedly provided on two support rods (2), the tops of the two support rods (2) are fixedly provided with lower centrifugal boxes (3), the top sides of the lower centrifugal boxes (3) are fixedly provided with connecting blocks (4) in a symmetrical manner, and the top sides of the four connecting blocks (4) are fixedly provided with upper centrifugal boxes (5); The liquid collection mechanism is arranged on the lower centrifugal box (3) and the upper centrifugal box (5) and is used to collect sample liquid in the lower centrifugal box (3) and the upper centrifugal box (5).

2. The device for measuring phytase activity in phytase fermentation liquid according to claim 1, wherein A motor (9) is fixedly mounted on the bottom side of the lower centrifugal box (3), an output end of the motor (9) passes through the bottom side of the lower centrifugal box (3) and extends to the interior of the lower centrifugal box (3), a driving rod (10) is fixedly arranged on the output end of the motor (9) through a coupling, a lower rotating drum (11) is fixedly arranged on the outer wall of the driving rod (10), the top end of the driving rod (10) passes through the top side of the inner wall of the lower centrifugal box (3) and the bottom side of the upper centrifugal box (5) in sequence and extends to the interior of the upper centrifugal box (5), and an upper rotating drum (12) is fixedly arranged on the top end of the driving rod (10).

3. The device for measuring phytase activity in phytase fermentation liquid according to claim 2, wherein: The liquid taking mechanism comprises two discharge pipes (13), two solenoid valves (14), two discharge pipes (15) and two valves (16); the two discharge pipes (13) are respectively connected to the outer walls of the lower drum (11) and the upper drum (12); each solenoid valve (14) is arranged on the outer wall of the discharge pipe (13); the two discharge pipes (15) are respectively connected to the outer walls of the lower centrifugal box (3) and the upper centrifugal box (5); and each valve (16) is arranged on the outer wall of the discharge pipe (15).

4. The device for measuring phytase activity in phytase fermentation liquid according to claim 3, wherein: The outer walls of the lower centrifugal box (3) and the upper centrifugal box (5) are both fixedly provided with supporting plates (8) corresponding to the discharge pipe (15), and the outer walls of the lower centrifugal box (3) and the upper centrifugal box (5) are respectively connected to the feed pipe (6) and the liquid inlet pipe (7).

5. The device for measuring phytase activity in phytase fermentation liquid according to claim 1, wherein: An installation groove (17) is provided inside the lower centrifugal box (3), and a plurality of electric heating tubes (18) are fixedly installed inside the installation groove (17).

6. The device for measuring phytase activity in phytase fermentation liquid according to claim 5, wherein: A spectrophotometer (20) for measuring phytase activity is provided on the top of the base (1).

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