Verification and collection device for plant growth promoting function of microbial volatile metabolites

Abstract

The utility model relates to a plant growth promoting function verification and collection device for microorganism volatile metabolites, which belongs to the technical field of microbiology, phytophysiology, biochemistry and agricultural biology and comprises a circular flowerpot, and a connecting device is arranged on the lower side of the circular flowerpot. The connecting device comprises a connecting flange connected to the lower side of the circular flowerpot through threads, three sets of filter membrane fixers fixedly connected to the interior of the connecting flange, two waterproof breathable membranes clamped between the filter membrane fixers and a filter membrane arranged between the two waterproof breathable membranes. A circular culture dish is in threaded connection with the lower side of the connecting flange. According to the plant growth promoting function verification and collection device for the microorganism volatile metabolites, the outer thread sleeves on the opposite sides of the circular flowerpot and the circular culture dish are in threaded connection with the inner wall of the connecting flange, and then the bottom of the circular culture dish is inserted into the circular opening, so that rapid assembly is achieved, and later disassembly is more convenient.

Description

Technical Field

[0001] The utility model relates to the fields of microbiology, plant physiology, biochemistry and agricultural biotechnology, and in particular is a device for verifying and collecting the plant growth-promoting function of microbial volatile metabolites. Background Art

[0002] The Plant Growth-Promoting Function Verification and Collection Device for Microbial Volatile Metabolites is a device specifically designed to study and verify the plant growth-promoting effects of microbial volatile metabolites. This type of device typically combines the functions of microbial cultivation, plant growth observation, and volatile compound collection and analysis.

[0003] The existing microbial volatile metabolite function verification and collection device has a complex structure and is difficult to disassemble and assemble, and cannot effectively verify the promotion of microbial volatile gases. At the same time, long-term use will cause internal contamination and lead to microbial infection. Therefore, a microbial volatile metabolite plant growth promotion function verification and collection device is proposed to solve the above problems. Utility Model Content

[0004] In response to the shortcomings of the existing technology, the utility model provides a device for verifying and collecting the plant growth-promoting function of microbial volatile metabolites, which has the advantages of easy disassembly and assembly, verification of the growth promotion of microbial volatile gases, and avoidance of microbial contamination. It solves the problem of complex structure and difficult disassembly and assembly, inability to effectively verify the growth promotion of microbial volatile gases, and internal contamination caused by long-term use, leading to microbial infection.

[0005] To achieve the above-mentioned object, the present utility model provides the following technical solutions: a device for verifying and collecting the plant growth-promoting function of microbial volatile metabolites, comprising a circular flower pot, wherein a connecting device is provided on the lower side of the circular flower pot;

[0006] The connecting device includes a connecting flange connected to the lower side of the circular flower pot by threads, three sets of filter membrane holders fixedly connected to the interior of the connecting flange, two waterproof and breathable membranes clamped between the filter membrane holders, and a filter membrane arranged between the two waterproof and breathable membranes. The threads on the lower side of the connecting flange are connected to a circular culture dish. The right side of the circular culture dish is connected to a microbial volatile gas collector. A gas collection assembly is arranged on the right side of the microbial volatile gas collector.

[0007] The gas collection assembly includes a headspace cover sleeved on the right side of the microbial volatile gas collector, an extraction needle detachably connected to the left inner wall of the headspace cover, an extraction fiber head inserted into the extraction needle, and a rubber gasket fixedly connected to the right inner wall of the microbial volatile gas collector.

[0008] Furthermore, a support assembly is provided on the lower side of the circular culture dish, and the support assembly includes a base sleeved on the lower side of the circular culture dish and an internal circular opening opened on the upper side of the base.

[0009] Furthermore, a plurality of through holes are opened inside the lower side of the circular flower pot, and the plurality of through holes are arranged at equal intervals in the horizontal direction.

[0010] Furthermore, the opposite sides of the circular flower pot and the circular culture dish are integrally connected with an outer screw sleeve, and the opposite sides of the circular flower pot and the circular culture dish are threadedly connected to the inner side of the connecting flange.

[0011] Furthermore, the two waterproof breathable membranes are arranged vertically inside the connecting flange, and the side edge of the filter membrane is clamped and fixed between the two waterproof breathable membranes by a filter membrane holder.

[0012] Furthermore, the outer side of the extraction needle passes through the rubber gasket and extends to the interior of the microbial volatile gas collector.

[0013] Furthermore, the circular flowerpot and the outer screw sleeve are communicated with each other, and the circular culture dish and the outer screw sleeve are communicated with each other.

[0014] Compared with the existing technology, the present invention provides a device for verifying and collecting the plant growth-promoting function of microbial volatile metabolites, which has the following beneficial effects:

[0015] 1. The device for verifying and collecting the plant growth-promoting function of microbial volatile metabolites is connected to the inner wall of the connecting flange through the outer screw sleeves on the opposite sides of the circular flowerpot and the circular culture dish, and then inserted into the circular opening from the bottom of the circular culture dish, thereby achieving rapid assembly and more convenient disassembly later.

[0016] 2. The device for verifying and collecting the plant growth-promoting function of microbial volatile metabolites, wherein the gas collection component is combined with the microbial volatile gas collector and inserted into the rubber gasket, can realize the collection of volatile gases, thereby effectively verifying the growth-promoting ability of microbial volatile metabolites and promoting plant growth and development, solving the problem of complex structure and difficult disassembly and assembly, which makes it impossible to effectively verify the growth-promoting ability of microbial volatile gases, and at the same time, causes internal contamination and leads to microbial infection when used for a long time. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a cross-sectional view of the structure of the utility model;

[0018] Figure 2 This is a structural sectional view of the connecting flange of the utility model;

[0019] Figure 3 This is a cross-sectional view of the structure of the gas collection component of the utility model;

[0020] Figure 4 This is a cross-sectional view of the structure of the utility model.

[0021] In the figure: 1 circular flower pot, 2 connecting flange, 3 circular culture dish, 4 outer screw sleeve, 5 gas collection assembly, 6 filter membrane holder, 7 filter membrane, 8 waterproof breathable membrane, 9 base, 10 circular opening, 11 through hole, 12 microbial volatile gas collector, 13 headspace cover, 14 rubber gasket, 15 extraction fiber head, 16 extraction needle. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0023] See also Figures 1 to 4 In this embodiment, a device for verifying and collecting the plant growth-promoting function of microbial volatile metabolites comprises a circular flowerpot 1, characterized by a connecting device disposed on the underside of the circular flowerpot 1. The connecting device comprises a connecting flange 2 threadedly connected to the underside of the circular flowerpot 1, three sets of filter membrane holders 6 fixedly attached to the interior of the connecting flange 2, two waterproof and breathable membranes 8 clamped between the filter membrane holders 6, and a filter membrane 7 disposed between the two waterproof and breathable membranes 8. A circular culture dish 3 is threadedly connected to the underside of the connecting flange 2. A microbial volatile gas collector 12 is connected to the right side of the circular culture dish 3. A gas collection assembly 5 is disposed on the right side of the microbial volatile gas collector 12. The gas collection assembly 5 comprises a headspace cover 13 sleeved onto the right side of the microbial volatile gas collector 12, a left extraction needle 16 detachably attached to the inner wall of the headspace cover 13, an extraction fiber head 15 inserted into the extraction needle 16, and a rubber gasket 14 fixedly attached to the right inner wall of the microbial volatile gas collector 12.

[0024] Among them, a support assembly is provided on the lower side of the circular culture dish 3, and the support assembly includes a base 9 sleeved on the lower side of the circular culture dish 3 and an internal circular opening 10 opened on the upper side of the base 9. A plurality of through holes 11 are opened inside the lower side of the circular flower pot 1, and the plurality of through holes 11 are arranged in a horizontally equidistant manner. The opposite sides of the circular flower pot 1 and the circular culture dish 3 are integrally connected with an outer screw sleeve 4, and the opposite sides of the circular flower pot 1 and the circular culture dish 3 are threadedly connected to the inner side of the connecting flange 2. Two waterproof and breathable membranes 8 are arranged vertically inside the connecting flange 2, and the side of the filter membrane 7 is clamped and fixed between the two waterproof and breathable membranes 8 by the filter membrane holder 6. The outer side of the extraction needle 16 passes through the rubber gasket 14 and extends to the inside of its microbial volatile gas collector 12. The circular flower pot 1 and the outer screw sleeve 4 are interconnected, and the circular culture dish 3 and the outer screw sleeve 4 are interconnected.

[0025] It should be noted that the filter membrane 7 is 0.22 microns, and the entire device is made of high-temperature resistant material, specifically high-temperature resistant glass, which is made of silicate glass and has excellent heat resistance and stability. The microbial volatile gas collector 12 includes a detachable extraction needle and an extraction fiber head, and the rubber gasket 14 in the gas collection assembly 5 ensures that it is not affected by external air.

[0026] The working principle of the above embodiment is:

[0027] The bottom of the circular culture dish 3 is inserted into the circular opening 10 opened by the base 9, and the outer screw sleeve 4 on the top of the circular culture dish 3 is threadedly connected to one end of the connecting flange 2, and then the circular flower pot 1 is taken out and threadedly connected to the inner wall of the other end of the connecting flange 2 through the outer screw sleeve 4 at one end. The volatile metabolites and microorganisms in the circular culture dish 3 are filtered out of the water remaining in the metabolites through the waterproof breathable membrane 8, and then enter the circular flower pot 1 through the through hole 11. When the microorganisms have a growth-promoting effect on the plants in the circular flower pot 1, it is necessary to collect and verify the volatile metabolites in the circular culture dish 3. When inoculating microorganisms, it is only necessary to insert and fix the extraction needle 16 inside the top space cover 13. As the microbial volatile gas collector 12 moves, the extraction needle 16 passes through the rubber gasket 14 and is inserted into the microbial volatile gas collector 12. The volatile metabolites are adsorbed from the extraction needle 16 to the surface of the extraction fiber head 15 for collection, thereby simplifying the operation. When microorganisms need to be inoculated, it is only necessary to add culture medium to the circular culture dish 3 to achieve the inoculation purpose. The addition of the filter membrane 7 and the waterproof and breathable membrane 8 can isolate the soil, moisture or other bacteria in the circular flower pot 1 from contacting the circular culture dish 3, thereby avoiding contamination by other microorganisms.

[0028] The installation method, connection method or setting method disclosed in this embodiment are all common mechanical connection methods, and can be implemented as long as they can achieve their beneficial effects.

[0029] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply the existence of any such actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article, or device comprising a series of elements includes not only those elements, but also other elements not explicitly listed, or elements inherent to such process, method, article, or device. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of other identical elements in the process, method, article, or device comprising the element.

[0030] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A device for verifying and collecting the plant growth-promoting function of microbial volatile metabolites, comprising a circular flower pot (1), characterized in that: A connecting device is provided on the lower side of the circular flower pot (1); The connecting device comprises a connecting flange (2) connected to the lower side of the circular flower pot (1) by means of a thread, three groups of filter membrane holders (6) fixedly connected to the interior of the connecting flange (2), two waterproof and breathable membranes (8) clamped between the filter membrane holders (6), and a filter membrane (7) arranged between the two waterproof and breathable membranes (8); a circular culture dish (3) is connected to the thread on the lower side of the connecting flange (2); a microbial volatile gas collector (12) is connected to the right side of the circular culture dish (3); and a gas collection assembly (5) is arranged on the right side of the microbial volatile gas collector (12); The gas collection assembly (5) comprises a headspace cover (13) sleeved on the right side of the microbial volatile gas collector (12), a left extraction needle (16) detachably connected to the inner wall of the headspace cover (13), an extraction fiber head (15) inserted into the extraction needle (16), and a rubber gasket (14) fixedly connected to the right inner wall of the microbial volatile gas collector (12).

2. The device for verifying and collecting the plant growth-promoting function of microbial volatile metabolites according to claim 1, characterized in that: A support assembly is provided on the lower side of the circular culture dish (3), and the support assembly comprises a base (9) sleeved on the lower side of the circular culture dish (3) and an internal circular opening (10) opened on the upper side of the base (9).

3. The device for verifying and collecting the plant growth-promoting function of microbial volatile metabolites according to claim 1, characterized in that: A plurality of through holes (11) are provided inside the lower side of the circular flower pot (1), and the plurality of through holes (11) are arranged at equal intervals in the transverse direction.

4. The device for verifying and collecting the plant growth-promoting function of microbial volatile metabolites according to claim 1, characterized in that: The opposite sides of the circular flower pot (1) and the circular culture dish (3) are integrally connected with an outer screw sleeve (4), and the opposite sides of the circular flower pot (1) and the circular culture dish (3) are threadedly connected to the inner side of the connecting flange (2).

5. The device for verifying and collecting the plant growth-promoting function of microbial volatile metabolites according to claim 1, characterized in that: The two waterproof breathable membranes (8) are arranged vertically inside the connecting flange (2), and the side of the filter membrane (7) is clamped and fixed between the two waterproof breathable membranes (8) by a filter membrane holder (6).

6. The device for verifying and collecting the plant growth-promoting function of microbial volatile metabolites according to claim 1, characterized in that: The outer side of the extraction needle (16) passes through the rubber gasket (14) and extends to the inside of the microbial volatile gas collector (12).

7. The device for verifying and collecting the plant growth-promoting function of microbial volatile metabolites according to claim 4, characterized in that: The circular flowerpot (1) and the outer screw sleeve (4) are in communication with each other, and the circular culture dish (3) and the outer screw sleeve (4) are in communication with each other.

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