Salmonella extraction device

Abstract

The utility model discloses a salmonella extraction device, which relates to the technical field of salmonella extraction, and comprises a culture dish and a sampling tube, the upper side of the culture dish is provided with a sampling port for sampling, the bottom end of the sampling tube is fixedly provided with a sampling nozzle, and the inner wall of the sampling tube is slidably provided with a sampling assembly; by rotating the adjusting ring, the adjusting ring moves downwards on the surface of the sampling tube through a thread line to drive the adjusting plate to move downwards, the adjusting plate drives the four supporting rods to move downwards, and the four supporting rods drive the adjusting block to slide in the adjusting groove, so that the sampling tube can be conveniently and rapidly adjusted, and the sampling tube can be conveniently and rapidly adjusted. Meanwhile, the adjusting blocks drive the baffles to rotate, so that the four baffles rotate by 90 degrees and are vertically spliced with one another, then the bottom is sealed by rotating the sealing plate, and the sealing plate is fixed among the four baffles through the first-stage magnetic strip and the second-stage magnetic strip, so that the sampling nozzle is sealed, the environment is prevented from being polluted, and subsequent recycling treatment is facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of salmonella extraction, in particular to a salmonella extraction device. Background Art

[0002] Salmonella is a common foodborne pathogen that is widely present in nature. It belongs to the Enterobacteriaceae family and is a Gram-negative bacillus. It can survive in feces, soil, food, and water for a long time, even up to 5 months to 2 years. After infection, it may cause acute gastroenteritis. In order to detect Salmonella, it is necessary to extract it first to avoid interference from other bacteria. The specific process is to use a sterile inoculation loop to pick an appropriate amount of suspected or mixed bacteria from a culture dish containing multiple bacteria, and inoculate the picked bacteria into the Difco Selenite In broth, the inoculation volume of each tube should be moderate to avoid being too dense. Place the inoculated enrichment solution in a 35±2℃ incubator for 18-24 hours. The time can be adjusted appropriately according to the activity of the bacteria. Take an appropriate amount (such as 10μL) from the enrichment solution and inoculate it onto the Salmonella chromogenic medium with a sterile inoculation loop. Use the three-zone line method or the coating method to evenly coat it. Place the inoculated medium in a 37℃ constant temperature incubator for 24-48 hours, observe the growth of the colonies, look for colonies with the characteristic color of Salmonella (such as purple) on the chromogenic medium, record their position and morphology, and use a sterile inoculation loop to select suspected Monoclonal purple colonies of Salmonella are inoculated onto new brain heart infusion agar medium (BHI) for streak purification. The inoculated BHI plates are placed in a 37°C constant temperature incubator for 24 hours to observe the purification effect and whether the morphology, color and other characteristics of the purified colonies meet the description of Salmonella. Finally, biochemical tests such as sugar fermentation test and urease test are carried out to further confirm its biochemical characteristics. Once it is confirmed to be Salmonella, a relatively pure culture solution full of Salmonella can be obtained. During the extraction process of this solution, an extraction device is required.

[0003] Publication No. CN219861336U discloses a Salmonella extraction device comprising a culture dish with a transparent glass lid secured to the top. The lid has a central through-hole, and the inner wall of the through-hole is fixedly connected to a wear-resistant rubber. A sampling hole is defined in the center of the rubber, and a sampling device is located within the rubber. During the entire sampling process, the user does not need to touch the Salmonella culture solution, making operation convenient and labor-saving. Furthermore, the elasticity of the wear-resistant rubber ensures the seal of the culture dish, preventing spillage and reducing the risk of infection.

[0004] However, this device still has some problems. The culture fluid in the culture dish is sucked into the space between the movable plate and the partition through the liquid collection needle, the sampling device is pulled out, and the pressing plate is pressed to squeeze the culture fluid out of the sampling device to complete the sampling. During the entire process, the user does not need to touch the Salmonella at all. However, in actual use, after the sampling is completed, the connecting tube and the liquid collection needle inserted in the culture dish will be directly exposed to the air. Salmonella can cause infection to the experimenters or pollute the environment through aerosols, which is not conducive to subsequent recovery and treatment. Therefore, we disclose a Salmonella extraction device to meet people's needs. Utility Model Content

[0005] The purpose of the present application is to provide a Salmonella extraction device to solve the problem raised in the above-mentioned background art that after complete sampling, the connecting tube and the liquid collection needle inserted into the culture dish will be directly exposed to the air, and Salmonella can cause infection to the experimenter or pollute the environment through aerosol, which is not conducive to subsequent recovery and processing.

[0006] To achieve the above objectives, the present application provides the following technical solution: a Salmonella extraction device, comprising a culture dish and a sampling tube, wherein the culture dish is provided with a sampling port for sampling on the upper side, the sampling tube is fixedly mounted with a sampling nozzle on the bottom end, a sampling assembly is slidably mounted on the inner wall of the sampling tube, and a sealing assembly for sealing the sampling nozzle is fixedly mounted on the surface of the sampling tube;

[0007] The sealing assembly includes a fixed plate, which is fixedly installed on the surface of the sampling tube. A plurality of baffles are rotatably installed on the side of the fixed plate, one side of which is rotatably installed with a sealing plate. One side of the sealing plate is arranged in an arc shape, and a flip unit for flipping the baffle is slidably installed on the surface of the sampling tube.

[0008] Preferably, the flipping unit includes an adjustment plate, which is slidably installed on the surface of the sampling tube, and a plurality of support rods are fixedly installed on the side of the adjustment plate. Adjustment grooves are provided on the upper sides of the plurality of baffles, and adjustment blocks are slidably installed in the adjustment grooves. The bottom ends of the plurality of support rods are rotatably connected to the upper sides of the plurality of adjustment blocks, and an adjustment ring is rotatably installed on the upper side of the adjustment plate. The surface of the sampling tube is provided with a threaded line, and the adjustment ring is screwed onto the surface of the sampling tube through the threaded line.

[0009] Preferably, the sampling assembly includes a limiting plate, which is slidably mounted on the inner wall of the sampling tube, a suction hole is opened on the lower side of the limiting plate, a suction needle for sampling is fixedly mounted in the suction hole, a piston is slidably mounted on the inner wall of the sampling tube, a sliding rod is fixedly mounted on the upper side of the piston, and an adjustment unit for moving the limiting plate up and down is rotatably mounted on the inner bottom wall of the sampling tube.

[0010] Preferably, the adjusting unit includes a threaded rod, which is rotatably mounted on the bottom end of the sampling tube, the limiting plate is threadedly mounted on the threaded area of ​​the threaded rod surface, the piston is slidably mounted on the non-threaded area of ​​the threaded rod surface, and the inner wall of the sampling tube is fixedly mounted with a limiting strip, and the upper sides of the limiting plate and the piston are both provided with limiting openings, and the limiting plate and the piston are slidably mounted on the surface of the limiting strip.

[0011] Preferably, a primary mounting groove is provided on the lower side of each of the baffles, a secondary magnetic strip is fixedly installed in the primary mounting groove, and a secondary mounting groove is provided on one side of the sealing plate, a primary magnetic strip is fixedly installed in the secondary mounting groove.

[0012] Preferably, a protective cover is fixedly mounted on the upper side of the sampling tube, and the sliding rod is slidably mounted on the protective cover.

[0013] Preferably, a vent is provided on the protective cover.

[0014] In summary, the technical effects and advantages of the utility model are:

[0015] 1. In the utility model, after the sampling is completed, the adjusting ring can be rotated, and the adjusting ring can move downward on the surface of the sampling tube through the thread line, driving the adjusting plate to move downward, and the adjusting plate drives the four support rods to move downward, and the four support rods drive the adjusting block to slide in the adjusting groove. At the same time, the adjusting block drives the baffle to rotate, so that the four baffles are rotated 90° and spliced ​​vertically with each other, and then the sealing plate is rotated to seal the bottom, and the sealing plate is fixed between the four baffles through the primary magnetic strip and the secondary magnetic strip, thereby completing the sealing of the sampling nozzle, preventing environmental pollution, and facilitating subsequent recycling and processing.

[0016] 2. In the utility model, the limit plate is driven to move by rotating the threaded rod and is driven downward by the action of the limit bar. The limit plate drives the suction needle to move downward, so that the suction needle extends out of the sampling nozzle. Then, by pulling the sliding rod, the sliding rod drives the piston to move upward, so that negative pressure is formed between the piston and the limit plate, and Salmonella is sampled through the suction needle. After the sampling is completed, the threaded rod is rotated in the opposite direction to retract the suction needle into the sampling nozzle, thereby reducing the volume of the device when it is stored and facilitating the sealing of the sampling nozzle. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present application. For those skilled in the art, other drawings can be obtained based on these drawings without paying any creative work.

[0018] Figure 1It is a schematic diagram of the three-dimensional structure of the utility model;

[0019] Figure 2 This is a schematic structural diagram of the sealing assembly in the present invention;

[0020] Figure 3 This is a schematic structural diagram of the baffle and sealing plate in the utility model;

[0021] Figure 4 This is a schematic diagram of the cross-sectional structure of the sampling tube in the present invention.

[0022] In the figure: 1. Petri dish; 2. Sampling tube; 3. Adjustment plate; 4. Support rod; 5. Baffle; 6. Adjustment block; 7. Sealing plate; 8. Adjustment ring; 9. Threaded line; 10. Fixed plate; 11. Primary magnetic strip; 12. Secondary magnetic strip; 13. Sampling nozzle; 14. Limiting piece; 15. Suction needle; 16. Limiting strip; 17. Threaded rod; 18. Piston; 19. Sliding rod; 20. Protective cover; 21. Vent. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0024] See also Figures 1-4 , Example 1 provided by the present utility model

[0025] The Salmonella extraction device includes a culture dish 1 and a sampling tube 2. The culture dish 1 has a sampling port for sampling on its upper side. The bottom end of the sampling tube 2 is fixedly mounted with a sampling nozzle 13. A sampling assembly is slidably mounted on the inner wall of the sampling tube 2. A sealing assembly for sealing the sampling nozzle 13 is fixedly mounted on the surface of the sampling tube 2.

[0026] The sealing assembly includes a fixed plate 10, which is fixedly installed on the surface of the sampling tube 2. A plurality of baffles 5 are rotatably installed on the side of the fixed plate 10, and a sealing plate 7 is rotatably installed on one side of one of the baffles 5. One side of the sealing plate 7 is set to an arc shape. A flip unit for flipping the baffle 5 is slidably installed on the surface of the sampling tube 2. The fixed plate 10 is rectangular, and baffles 5 are rotatably installed on all four sides. A total of four baffles 5 are rotatably installed. The baffles 5 can be flipped downward by a hinge installation for splicing and sealing.

[0027] like Figure 2 and Figure 3As shown, the flip unit includes an adjusting plate 3, which is slidably mounted on the surface of the sampling tube 2, and a plurality of support rods 4 are fixedly mounted on the side of the adjusting plate 3. Adjustment grooves are provided on the upper sides of the plurality of baffles 5, and adjustment blocks 6 are slidably mounted in the adjustment grooves. The bottom ends of the plurality of support rods 4 are rotatably connected to the upper sides of the plurality of adjustment blocks 6, and an adjusting ring 8 is rotatably mounted on the upper side of the adjusting plate 3. The surface of the sampling tube 2 is provided with a threaded line 9, and the adjusting ring 8 is screwed and mounted on the surface of the sampling tube 2 through the threaded line 9. The inner wall of the adjusting ring 8 is provided with a threaded groove, which is screwed and mounted through the threaded groove and the threaded line 9 on the surface of the sampling tube 2. The size of the adjusting plate 3 is consistent with that of the fixed plate 10, and the adjusting block 6 is rotatably mounted on the bottom ends of the four support rods 4 mounted on the side. When the support rod 4 moves downward, the adjusting block 6 will be driven to move downward, and the adjusting block 6 will move along the adjustment groove, which will drive the baffle 5 to gradually flip downward.

[0028] After the sampling is completed, the adjusting ring 8 can be rotated, and the adjusting ring 8 can move downward on the surface of the sampling tube 2 through the thread line 9, driving the adjusting plate 3 to move downward, and the adjusting plate 3 drives the four support rods 4 to move downward, and the four support rods 4 drive the adjusting block 6 to slide in the adjusting groove. At the same time, the adjusting block 6 drives the baffle 5 to rotate, so that the four baffles 5 are rotated 90° and spliced ​​vertically with each other, and then the sealing plate 7 is rotated to seal the bottom, and the sealing plate 7 is fixed between the four baffles 5 through the primary magnetic strip 11 and the secondary magnetic strip 12, completing the sealing of the sampling mouth 13 to prevent environmental pollution and facilitate subsequent recycling.

[0029] Example 2

[0030] like Figure 4 As shown, the sampling assembly includes a limit plate 14, which is slidably mounted on the inner wall of the sampling tube 2, and a suction hole is provided on the lower side of the limit plate 14, in which a suction needle 15 for sampling is fixedly installed, a piston 18 is slidably mounted on the inner wall of the sampling tube 2, a slide rod 19 is fixedly mounted on the upper side of the piston 18, and an adjustment unit for moving the limit plate 14 up and down is rotatably mounted on the inner bottom wall of the sampling tube 2, an avoidance hole is provided on the upper side of the piston 18, the avoidance hole is used to avoid the threaded rod 17, and a rubber ring is provided in the avoidance hole, which can fit on the surface of the threaded area when in contact with the threaded area on the surface of the threaded rod 17 to seal and ensure normal sampling.

[0031] like Figure 4 As shown, the adjustment unit includes a threaded rod 17, which is rotatably mounted on the bottom end of the sampling tube 2, a limiting plate 14 is screwed on the threaded area of ​​the threaded rod 17, and a piston 18 is slidably mounted on the non-threaded area of ​​the threaded rod 17. A limiting strip 16 is fixedly mounted on the inner wall of the sampling tube 2, and limiting openings are provided on the upper sides of the limiting plate 14 and the piston 18, and the limiting plate 14 and the piston 18 are slidably mounted on the surface of the limiting strip 16.

[0032] like Figure 3 As shown, a primary mounting groove is provided on the lower side of each of the baffles 5, in which a secondary magnetic strip 12 is fixedly installed. A secondary mounting groove is provided on one side of the sealing plate 7, in which a primary magnetic strip 11 is fixedly installed. When the sealing plate 7 completes its rotation, the secondary magnetic strip 12 and the primary magnetic strip 11 will attract each other and be fixed.

[0033] like Figure 2 As shown, a protective cover 20 is fixedly installed on the upper side of the sampling tube 2, and the slide rod 19 is slidably installed on the protective cover 20. An air vent 21 is provided on the protective cover 20. The protective cover 20 prevents the piston 18 from separating from the sampling tube 2. The air vent 21 is used to balance the air pressure when the piston 18 is extracted to prevent the piston 18 from being unable to extract.

[0034] Rotating the threaded rod 17 drives the limiting plate 14 to move and drives the limiting plate 14 to move downward under the action of the limiting bar 16. The limiting plate 14 drives the suction needle 15 to move downward, so that the suction needle 15 extends out of the sampling nozzle 13, and then by pulling the sliding rod 19, the sliding rod 19 drives the piston 18 to move upward, so that a negative pressure is formed between the piston 18 and the limiting plate 14, and the Salmonella is sampled through the suction needle 15. After the sampling is completed, the threaded rod 17 is rotated in the opposite direction to retract the suction needle 15 into the sampling nozzle 13, reducing the volume of the device when it is stored, which is convenient for sealing the sampling nozzle 13.

[0035] Finally, it should be noted that the above are only preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent replacements for some of the technical features therein. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A Salmonella extraction device, comprising a culture dish (1) and a sampling tube (2), characterized in that: A sampling port for sampling is provided on the upper side of the culture dish (1); a sampling nozzle (13) is fixedly mounted on the bottom end of the sampling tube (2); a sampling assembly is slidably mounted on the inner wall of the sampling tube (2); and a sealing assembly for sealing the sampling nozzle (13) is fixedly mounted on the surface of the sampling tube (2); The sealing assembly comprises a fixed plate (10), the fixed plate (10) being fixedly mounted on the surface of the sampling tube (2), a plurality of baffles (5) being rotatably mounted on the side of the fixed plate (10), a sealing plate (7) being rotatably mounted on one side of one of the baffles (5), one side of the sealing plate (7) being arranged in an arc shape, and a flip unit for flipping the baffle (5) being slidably mounted on the surface of the sampling tube (2).

2. The Salmonella extraction device according to claim 1, characterized in that: The flip unit comprises an adjustment plate (3), the adjustment plate (3) is slidably mounted on the surface of the sampling tube (2), a plurality of support rods (4) are fixedly mounted on the side of the adjustment plate (3), the upper sides of the plurality of baffles (5) are provided with adjustment grooves, an adjustment block (6) is slidably mounted in the adjustment groove, the bottom ends of the plurality of support rods (4) are rotatably connected to the upper sides of the plurality of adjustment blocks (6), an adjustment ring (8) is rotatably mounted on the upper side of the adjustment plate (3), a threaded line (9) is provided on the surface of the sampling tube (2), and the adjustment ring (8) is screwed and mounted on the surface of the sampling tube (2) through the threaded line (9).

3. The Salmonella extraction device according to claim 1, characterized in that: The sampling assembly includes a limiting piece (14), which is slidably mounted on the inner wall of the sampling tube (2), a suction hole is provided on the lower side of the limiting piece (14), a suction needle (15) for sampling is fixedly mounted in the suction hole, a piston (18) is slidably mounted on the inner wall of the sampling tube (2), a sliding rod (19) is fixedly mounted on the upper side of the piston (18), and an adjustment unit for moving the limiting piece (14) up and down is rotatably mounted on the inner bottom wall of the sampling tube (2).

4. The Salmonella extraction device according to claim 3, characterized in that: The adjusting unit comprises a threaded rod (17), the threaded rod (17) is rotatably mounted on the bottom end of the sampling tube (2), the limiting piece (14) is threadedly mounted on the threaded area of ​​the threaded rod (17), the piston (18) is slidably mounted on the non-threaded area of ​​the threaded rod (17), the inner wall of the sampling tube (2) is fixedly mounted with a limiting strip (16), the upper sides of the limiting piece (14) and the piston (18) are both provided with limiting openings, and the limiting piece (14) and the piston (18) are slidably mounted on the surface of the limiting strip (16).

5. The Salmonella extraction device according to claim 1, characterized in that: A primary installation groove is provided on the lower side of each of the baffles (5), a secondary magnetic strip (12) is fixedly installed in the primary installation groove, and a secondary installation groove is provided on one side of the sealing plate (7), a primary magnetic strip (11) is fixedly installed in the secondary installation groove.

6. The Salmonella extraction device according to claim 3, characterized in that: A protective cover (20) is fixedly mounted on the upper side of the sampling tube (2), and the sliding rod (19) is slidably mounted on the protective cover (20).

7. The Salmonella extraction device according to claim 6, characterized in that: The protective cover (20) is provided with a vent (21).

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