Organ-like culture support

Abstract

The utility model discloses an organ-like culture support, which relates to the technical field of biological cell culture and comprises a culture dish, a groove is formed in the top of the culture dish, sliding rails are fixedly connected to the left side wall and the right side wall of the top of the groove, a plurality of culture supports are arranged between the two sliding rails, and guide grooves are formed in the left side and the right side of the top of the culture dish. Guide rods are slidably mounted in the two guide grooves, limiting blocks movably sleeve the surfaces of the two guide rods, limiting grooves are formed in the tops of the two sliding rails, cross rods are fixedly connected to the front side and the rear side of the culture dish, and splicing assemblies are arranged at the right ends of the two cross rods. Through the arrangement of the sliding rails and the culture brackets, when cells grow all over one bracket body, the culture layers are driven by the sliding plates to be connected with the previous culture layer along the sliding rails, and the connection stability is ensured through the limiting blocks, so that the purposes of migration, proliferation and differentiation of the cells are achieved, and the culture efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of biological cell culture, in particular to an organoid culture scaffold. Background Art

[0002] Organoids are in vitro organ models developed from three-dimensional cell aggregates of stem cells or adult tissues. They can highly simulate the physiological structure, function, development and maintenance process of in situ tissues. They have the advantage of genomic stability during long-term in vitro expansion and can be applied to the study of disease mechanisms, drug screening, tissue engineering, and transplantation therapy.

[0003] An organoid culture scaffold is a basic structure used for in vitro culture and development of organoids, used to support and promote cell growth, differentiation and tissue formation. Currently, most traditional organoid culture scaffolds are placed independently in individual bases. Once cells fill a scaffold body, they can no longer continue to proliferate and the scaffold needs to be replaced for continued culture. This is not flexible enough to use and has certain limitations. Utility Model Content

[0004] In order to solve the above technical problems, an organoid culture scaffold is provided. This technical solution solves the problem proposed in the above background technology that most traditional organoid culture scaffolds are placed independently in individual bases. When the cells fill up a scaffold body, they can no longer continue to proliferate and the scaffold needs to be replaced before culture can continue. The use is not flexible and has certain limitations.

[0005] In order to achieve the above purpose, the technical solution adopted by this utility model is:

[0006] An organoid culture support comprises a culture dish, a groove is provided on the top of the culture dish, and slide rails are fixedly connected to the left and right side walls of the top of the groove, and several culture supports are arranged between the two slide rails. Guide grooves are provided on the left and right sides of the top of the culture dish, and guide rods are slidably installed inside the two guide grooves. L-shaped limit blocks are movably sleeved on the surfaces of the two guide rods. Limit grooves are provided on the tops of the two slide rails, and the ends of the two limit blocks close to each other are respectively slidably connected to the two limit grooves. Cross bars are fixedly connected to the front and back sides of the culture dish, and splicing components are provided at the right ends of the two cross bars.

[0007] Optionally, the culture support includes a pair of slides, and the two slides are slidably installed inside the two slide rails respectively. The sides of the two slides close to each other are fixedly connected to a connecting plate, and the bottoms of the two connecting plates are fixedly connected to several culture layers.

[0008] Optionally, the tops of the two limit blocks are threadedly connected with fixing bolts, and gaskets are provided between the two fixing bolts and the limit blocks. The ends of the two limit blocks that are close to each other are respectively in contact with the front ends of the two slides.

[0009] Optionally, the splicing assembly includes a pair of slide grooves, the two slide grooves are respectively opened on the side where the two cross bars are close to each other, the inside of the two slide grooves are slidably connected with a card block, the ends of the two card blocks away from each other are fixedly connected with a spring, the other ends of the two springs are fixedly connected to the inner wall of the slide groove, the top of the two slide grooves are provided with a through groove, the top of the two card blocks are fixedly connected with a shift block, the upper ends of the two shift blocks extend out through the two through grooves and are slidably connected to the inside of the through groove, and the front and rear side walls of the culture dish are provided with card slots.

[0010] Optionally, transparent observation windows are provided on both the front and rear side walls of the culture dish.

[0011] Compared with the prior art, the beneficial effects of the present invention are:

[0012] 1. This solution is equipped with a slide rail and a culture scaffold. When the cells have grown all over a scaffold body, the slide is used to drive the culture layer along the slide rail to connect with the previous culture layer, and the limit block ensures the stability of the connection, thereby achieving the purpose of cell migration, proliferation and differentiation, and improving the culture efficiency.

[0013] 2. This solution is equipped with a splicing component, which uses the card block in the previous culture dish and the card slot in the next culture dish to engage with each other, so that multiple culture dishes can be spliced ​​together in sequence, which is convenient for staff to observe and compare. It has a simple structure and is easy to operate, which improves the practicality of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the three-dimensional structure of the utility model;

[0015] Figure 2 This is a sectional view of the three-dimensional structure of the utility model;

[0016] Figure 3 for Figure 2 A partial enlarged schematic diagram of point A in the middle;

[0017] Figure 4 for Figure 2 A partial enlarged schematic diagram of point B in the middle.

[0018] The numbers in the figure are:

[0019] 1. Culture dish; 2. Groove; 3. Slide rail; 4. Culture bracket; 41. Slide plate; 42. Connecting plate; 43. Culture layer; 5. Guide groove; 6. Guide rod; 7. Limit block; 71. Fixing bolt; 72. Gasket; 8. Limit groove; 9. Cross bar; 10. Splicing assembly; 101. Slide groove; 102. Block; 103. Spring; 104. Through groove; 105. Dial block; 106. Slot; 11. Transparent observation window. DETAILED DESCRIPTION

[0020] The following description is intended to disclose the present invention so that those skilled in the art can implement the present invention. The preferred embodiments described below are merely examples, and those skilled in the art may conceive of other obvious variations.

[0021] Reference Figure 1 As shown, an organoid culture scaffold comprises a culture dish 1, a groove 2 is provided on the top of the culture dish 1, and slide rails 3 are fixedly connected to the left and right side walls of the top of the groove 2, and a number of culture scaffolds 4 are provided between the two slide rails 3. Guide grooves 5 are provided on the left and right sides of the top of the culture dish 1, and guide rods 6 are slidably installed inside the two guide grooves 5. L-shaped limit blocks 7 are movably sleeved on the surfaces of the two guide rods 6. Limit grooves 8 are provided on the tops of the two slide rails 3, and the ends of the two limit blocks 7 close to each other are slidably connected to the two limit grooves 8 respectively. The front and back sides of the culture dish 1 are fixedly connected. The cross bar 9 and the right ends of the two cross bars 9 are each provided with a splicing assembly 10. Before use, according to the culture requirements, the required number of culture dishes 1 are spliced ​​together by engaging the card block 102 in the splicing assembly 10 with the card slot 106. When the cells have grown a full support body, the new culture support 4 is moved along the slide rail 3 to the front end of the previous culture support 4, so that the culture layer 43 is connected to the previous culture layer 43, and the limit block 7 is lowered along the guide rod 6 and abutted against the front end of the slide 41, ensuring the stability of the connection of the culture layer 43, allowing the cells to migrate, proliferate, and differentiate, thereby improving the culture efficiency.

[0022] Further, such as Figure 2-Figure 4As shown, the culture support 4 includes a pair of slides 41, and the two slides 41 are slidably installed inside the two slide rails 3 respectively. The sides of the two slides 41 that are close to each other are fixedly connected with a connecting plate 42, and the bottoms of the two connecting plates 42 are fixedly connected with several culture layers 43. The tops of the two limit blocks 7 are threadedly connected with fixing bolts 71, and gaskets 72 are provided between the two fixing bolts 71 and the limit blocks 7. The ends of the two limit blocks 7 that are close to each other are respectively abutted against the front ends of the two slides 41, and the connecting plates 42 are driven by the slides 41 to move along the slide rails 3, and the connecting plates 42 drive the several culture layers 43 at the bottom to be connected with the upper culture layer 43, and the guide rod 6 is moved. The guide rod 6 drives the limit block 7 to move along the guide groove 5, and the limit block 7 is rotated to one side of the limit groove 8 and is lowered along the guide rod 6. The end of the limit block 7 passes through the limit groove 8 and abuts against the front end of the slide 41. The fixing bolt 71 is rotated to fix the limit block 7, and the connection between the culture layers 43 is made more stable by squeezing the slide 41.

[0023] The splicing assembly 10 includes a pair of slides 101, the two slides 101 are respectively opened on the side where the two cross bars 9 are close to each other, the inside of the two slides 101 are slidably connected with a card block 102, the ends of the two card blocks 102 away from each other are fixedly connected with a spring 103, the other ends of the two springs 103 are fixedly connected to the inner wall of the slide 101, the tops of the two slides 101 are respectively provided with a through slot 104, the tops of the two card blocks 102 are fixedly connected with a dial block 105, the upper ends of the two dial blocks 105 respectively extend out through the two through slots 104 and are slidably connected to the inside of the through slot 104 The front and rear side walls of the culture dish 1 are both provided with card slots 106, and the front and rear side walls of the culture dish 1 are both provided with transparent observation windows 11. When splicing the culture dishes 1, the culture dishes 1 are moved along the cross bar 9 of the previous culture dish 1 by squeezing the card block 102. When the two culture dishes 1 are tightly fitted together, the card block 102 in the previous culture dish 1 pops out from the slide 101 under the action of the spring 103 and extends into the card slot 106 in the next culture dish 1. The two culture dishes 1 are spliced ​​together by the card block 102 and the card slot 106. The structure is simple, the operation is convenient, and the practicality of the device is improved.

[0024] The working principle of the present invention is as follows: before use, according to the culture requirements, several culture dishes 1 are spliced ​​together through the splicing assembly 10, and a culture bracket 4 is fixed in each culture dish 1. Then, culture medium is added to each groove 2 to start culture. When the cells have grown all over a bracket body, a new culture bracket 4 is connected along the slide rail 3, and the limit block 7 is readjusted so that the limit block 7 abuts against the front end of the slide plate 41 in the new culture bracket 4. The fixing bolt 71 is tightened to complete the connection of the new bracket, allowing the cells to migrate, proliferate and differentiate.

[0025] The above shows and describes the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions merely illustrate the principles of the present invention. Various changes and improvements are possible without departing from the spirit and scope of the present invention. Such changes and improvements are intended to fall within the scope of the present invention. The scope of protection claimed for the present invention is defined by the appended claims and their equivalents.

Claims

1. An organoid culture scaffold, characterized in that: The invention comprises a culture dish (1), wherein a groove (2) is provided on the top of the culture dish (1), and slide rails (3) are fixedly connected to the left and right side walls of the top of the groove (2), and a plurality of culture supports (4) are provided between the two slide rails (3). Guide grooves (5) are provided on the left and right sides of the top of the culture dish (1), and guide rods (6) are slidably installed inside the two guide grooves (5). L-shaped limit blocks (7) are movably sleeved on the surfaces of the two guide rods (6), and limit grooves (8) are provided on the tops of the two slide rails (3). The ends of the two limit blocks (7) close to each other are slidably connected to the two limit grooves (8), and the front and rear sides of the culture dish (1) are fixedly connected to cross bars (9), and the right ends of the two cross bars (9) are provided with splicing components (10).

2. The organoid culture scaffold according to claim 1, characterized in that: The culture support (4) includes a pair of slides (41), the two slides (41) are slidably mounted inside the two slide rails (3), and the two slides (41) are fixedly connected to a connecting plate (42) on the side close to each other, and a plurality of culture layers (43) are fixedly connected to the bottom of the two connecting plates (42).

3. The organoid culture scaffold according to claim 1, characterized in that: The tops of the two limit blocks (7) are both threadedly connected with fixing bolts (71), and gaskets (72) are provided between the two fixing bolts (71) and the limit blocks (7). The ends of the two limit blocks (7) that are close to each other are respectively in contact with the front ends of the two slide plates (41).

4. The organoid culture scaffold according to claim 1, characterized in that: The splicing assembly (10) includes a pair of slide grooves (101), the two slide grooves (101) are respectively opened on the side of the two cross bars (9) close to each other, the interior of the two slide grooves (101) is slidably connected with a clamping block (102), the ends of the two clamping blocks (102) away from each other are fixedly connected with a spring (103), the other ends of the two springs (103) are fixedly connected to the inner wall of the slide groove (101), the tops of the two slide grooves (101) are respectively provided with a through groove (104), the tops of the two clamping blocks (102) are fixedly connected with a shifting block (105), the upper ends of the two shifting blocks (105) extend out through the two through grooves (104) and are slidably connected to the interior of the through groove (104), and the front and rear side walls of the culture dish (1) are both provided with a clamping groove (106).

5. The organoid culture scaffold according to claim 1, characterized in that: Transparent observation windows (11) are provided on both the front and rear side walls of the culture dish (1).

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