Biological enzyme culture stirring device

Abstract

The utility model provides a bio-enzyme culture stirring device, which relates to the field of stirring devices and comprises a culture tank, two sides of the culture tank are rotatably connected with a support, the bottom of the culture tank is provided with a discharge port, the upper side of the culture tank is provided with an adjusting assembly, the adjusting assembly comprises an air pump, the outlet end of the air pump is connected with an air pipe, and the air pipe is connected with a water pump. Meanwhile, the outer end of the air pipe is inserted into the culture tank, a transverse rod is arranged in the culture tank, the lower side of the transverse rod is connected with a stirring assembly, a through hole is formed in the transverse rod, the stirring assembly comprises an insulating pipe, a heat conduction sleeve is arranged in the insulating pipe, and an electric heating pipe is arranged in the heat conduction sleeve. The stirring device for biological enzyme culture solves the problems that the existing stirring device for biological enzyme culture affects biological enzyme culture while realizing gas stirring, and the stirring uniformity is deviated.

Description

Technical Field

[0001] The utility model relates to the technical field of stirring devices, in particular to a biological enzyme culture stirring device. Background Art

[0002] Existing enzymes are typically produced through microbial fermentation. This involves culturing microorganisms that have been introduced with enzyme genes. Once the microorganisms have reached a predetermined stage of development, they begin producing enzymes. After the culture reaches a certain level, the culture medium is extracted, filtered, and purified to obtain the corresponding enzyme.

[0003] After searching, the prior art (publication number: CN216639460U) describes a "biological enzyme culture stirring device, comprising a culture tank body, with a feed port mounted on the top and a discharge port mounted on the bottom, both of which are equipped with valves. An annular plate is fixed to the culture tank body, forming a ventilation space between the annular plate and the inner wall of the culture tank body; the annular plate is formed with a plurality of through holes, and arc-shaped elastic sheets are fixed on both sides of the through holes, and the arc-shaped elastic sheets are arranged in close proximity; the top of the culture tank body is connected to the ventilation space via a circulation pipe; and a circulation pump is installed on the circulation pipe. This utility model has a simple structure and is easy to use. It reduces mechanical damage to the bacterial strain through gas stirring and effectively prevents the culture solution from flowing back through the gas outlet."

[0004] Although the bio-enzyme culture stirring device in the existing technology realizes the gas stirring method, it still has some shortcomings: the existing bio-enzyme culture stirring device uses circulating gas stirring, which easily causes changes in the temperature and air oxygen content inside the culture tank, and thus does not utilize the bio-enzyme culture. Secondly, the culture liquid at the bottom of the culture tank cannot be stirred by the gas, resulting in deviation in the uniformity of the stirring. Utility Model Content

[0005] In order to overcome the defects of the existing technology, a biological enzyme culture stirring device is now provided to solve the problem that the existing biological enzyme culture stirring device affects the cultivation of biological enzymes while achieving gas stirring, and there is a deviation in stirring uniformity.

[0006] To achieve the above-mentioned purpose, a biological enzyme culture stirring device is provided, comprising: a culture tank, wherein the two sides of the culture tank are rotatably connected to the bracket, and the bottom of the culture tank is provided with a discharge port, the upper side of the culture tank is provided with an adjustment component, and the adjustment component includes an air pump, and the outlet end of the air pump is connected to an air pipe, and the outer end of the air pipe is inserted into the culture tank, the interior of the culture tank is provided with a cross bar, and the lower side of the cross bar is connected to the stirring component, and the interior of the cross bar is provided with a through hole, the stirring component includes an insulating tube, and a heat-conducting sleeve is provided inside the insulating tube, and an electric heating tube is provided inside the heat-conducting sleeve.

[0007] Furthermore, a motor and an electric control box are installed on the outside of the bracket, and the output shaft end of the motor is transmission-connected to the shaft rod at the outer end of the culture tank through a gear set.

[0008] Furthermore, the air inlet end of the air pump is connected to a dust cover, and a dust net is provided inside the dust cover, and one end of the air pipe is connected to the air outlet end of the air pump, and a flow valve is installed in the air pipe.

[0009] Furthermore, the cross bar is installed along the length direction of the culture tank, and both ends of the cross bar are fixed to the inner walls on both sides of the culture tank.

[0010] Furthermore, a temperature sensor and an oxygen content sensor are installed on the outer wall of the culture tank, and the oxygen content sensor is electrically connected to the air pump through an electric control box.

[0011] Furthermore, the electric heating tube is connected to an external thermostat via a wire, and the thermostat is electrically connected to a temperature sensor, and connecting rods are connected between the multiple groups of insulating tubes.

[0012] Furthermore, the inner wall of the culture tank is provided with a flow hole connected to the outside, and the inner port of the flow hole is provided with a waterproof breathable membrane, the outer port of the flow hole is installed with a one-way valve, and a metal grid is provided between the waterproof breathable membrane and the one-way valve.

[0013] The beneficial effects of the present invention are:

[0014] 1. When in use, the bio-enzyme culture solution is put into the culture tank through the discharge port, and then the discharge port is closed. The driving motor drives the culture tank to rotate slowly between the brackets through the gear set to keep the internal culture solution in a flowing state. Then, the insulating tube, connecting rod, and cross bar included in the stirring assembly are used to achieve the effect of internal stirring of the flowing culture solution. This state of stirring does not generate strong shear force on the culture solution, and at the same time achieves a more uniform stirring effect.

[0015] 2. The heat-conducting sleeve and electric heating tube included in the stirring assembly are used to heat the culture solution at a constant temperature while stirring. At the same time, the temperature is controlled by a temperature sensor and a temperature controller. Then, the oxygen content inside the culture tank is detected by an oxygen content sensor. When the internal oxygen content decreases, the air pump is automatically started to supply air to the inside of the culture tank through the air pipe. During the process, the gas is discharged through the flow hole to maintain the stability of the temperature and oxygen content inside the culture tank. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the front cross-section structure of an embodiment of the present utility model.

[0017] Figure 2This is a schematic structural diagram of a stirring assembly according to an embodiment of the present invention.

[0018] Figure 3 This is a structural diagram of point A in Figure 1 of an embodiment of the present utility model.

[0019] Figure 4 This is a schematic diagram of the structure of the adjustment component of an embodiment of the present utility model.

[0020] In the figure: 1. Culture tank; 11. Discharge port; 12. Bracket; 13. Temperature sensor; 14. Oxygen sensor; 15. Flow hole; 151. Waterproof and breathable membrane; 152. Metal grid; 153. One-way valve; 2. Adjustment component; 21. Air pump; 22. Dust cover; 23. Dust net; 24. Flow valve; 25. Air pipe; 3. Cross bar; 31. Through hole; 4. Stirring component; 41. Insulation tube; 42. Heat-conducting sleeve; 43. Electric heating tube; 44. Connecting rod; 5. Motor; 51. Gear set; 6. Electric control box. DETAILED DESCRIPTION

[0021] Reference Figures 1 to 4 As shown, the utility model provides a biological enzyme culture stirring device, comprising: a culture tank 1, a rotatable connecting bracket 12 on both sides of the culture tank 1, and a discharge port 11 is provided at the bottom of the culture tank 1, an adjusting component 2 is provided on the upper side of the culture tank 1, and the adjusting component 2 includes an air pump 21, and the outlet end of the air pump 21 is connected to an air pipe 25, and the outer end of the air pipe 25 is inserted into the culture tank 1, a cross bar 3 is provided inside the culture tank 1, and a stirring component 4 is connected to the lower side of the cross bar 3, and a through hole 31 is opened inside the cross bar 3, the stirring component 4 includes an insulating tube 41, and a heat-conducting sleeve 42 is provided inside the insulating tube 41, and an electric heating tube 43 is provided inside the heat-conducting sleeve 42.

[0022] In this embodiment, the culture tank 1 and the inner and outer components constitute the main structure of the biological enzyme culture stirring device involved in this application.

[0023] Specifically, the culture tank 1 is configured as a cylindrical structure, and the inner wall located on the side of the discharge port 11 is configured as a conical flow-guiding inner wall structure.

[0024] like Figure 1 and Figure 4 As shown, a motor 5 and an electric control box 6 are installed on the outside of the bracket 12, and the output shaft end of the motor 5 is connected to the outer end shaft of the culture tank 1 through a gear set 51, the air inlet end of the air pump 21 is connected to a dust cover 22, and a dust net 23 is provided inside the dust cover 22, and one end of the air pipe 25 is connected to the air outlet end of the air pump 21, and a flow valve 24 is installed in the pipeline of the air pipe 25.

[0025] Specifically, the output shaft end of the motor 5 is connected to a gear, and the shaft rod at the outer end of the culture tank 1 is connected to a gear, and the two sets of gears are meshed with each other to form a gear set 51.

[0026] Specifically, during the oxygen injection process, the motor 5 rotates the culture tank 1 to a state where the air pump 21 is located on the culture tank 1 , and then controls the flow valve 24 to open, and then starts the air pump 21 .

[0027] It should be noted that the culture medium inside the culture tank 1 does not completely fill the internal space, so that when oxygen is injected, air enters the upper space of the culture medium, and then the flow valve 24 is closed, and the motor 5 is started, so that the culture medium flows and turns to contact with the air to realize the oxygen dissolution process. At the same time, when the flow hole 15 is upward and separated from the culture medium, part of the air inside can be discharged through the flow hole 15.

[0028] like Figures 1 to 3 As shown, the cross bar 3 is installed along the length direction of the culture tank 1, and the two ends of the cross bar 3 are fixed to the inner walls on both sides of the culture tank 1. The outer wall of the culture tank 1 is installed with a temperature sensor 13 and an oxygen content sensor 14. The oxygen content sensor 14 is electrically connected to the air pump 21 through the electrical control box 6. The electric heating tube 43 is connected to the temperature controller on the outside through a wire, and the temperature controller is electrically connected to the temperature sensor 13. A connecting rod 44 is connected between the multiple groups of insulating tubes 41. The inner wall of the culture tank 1 is provided with a flow hole 15 connected to the outside, and the inner port of the flow hole 15 is provided with a waterproof and breathable membrane 151. The outer port of the flow hole 15 is installed with a one-way valve 153, and a metal grid 152 is provided between the waterproof and breathable membrane 151 and the one-way valve 153.

[0029] Specifically, the internal through hole 31 of the cross bar 3 realizes the turbulence effect on the flipped culture solution, further improving the uniformity of stirring.

[0030] As a preferred embodiment, the waterproof breathable membrane 151 is provided to prevent the outflow of culture fluid and facilitate the communication between the internal space of the culture tank 1 and the outside world, and the metal grid 152 is used to support the waterproof breathable membrane 151.

[0031] During use, the bio-enzyme culture solution is put into the culture tank through the discharge port, and then the discharge port is closed. The driving motor drives the culture tank to rotate slowly between the brackets through the gear set, so that the culture solution inside is kept in a flowing state. Then, the insulating tube, connecting rod, and cross bar included in the stirring assembly are used to achieve the effect of internal stirring of the flowing culture solution. At the same time, the heat-conducting sleeve and electric heating tube included in the stirring assembly are used to heat the culture solution at a constant temperature while stirring. At the same time, the temperature is controlled by the temperature sensor and temperature control. Then, the oxygen content inside the culture tank is detected by the oxygen content sensor. When the internal oxygen content is reduced, the air pump is automatically started to supply gas to the inside of the culture tank through the air pipe. During the process, the gas is discharged through the flow hole, so as to maintain the temperature and oxygen content inside the culture tank stable.

[0032] The bio-enzyme culture stirring device of the present invention can effectively solve the problem that the existing bio-enzyme culture stirring device affects the cultivation of bio-enzymes while achieving gas stirring, and there is a deviation in stirring uniformity. It achieves a more comprehensive and uniform stirring effect on the basis of the existing bio-enzyme culture stirring device technology, and is convenient for detecting and adjusting the temperature and oxygen content of the internal culture solution, so that the bio-enzyme culture is in a specific culture environment.

Claims

1. A biological enzyme culture stirring device, comprising: A culture tank (1), wherein both sides of the culture tank (1) are rotatably connected to brackets (12), and a discharge port (11) is provided at the bottom of the culture tank (1), characterized in that: an adjustment component (2) is provided on the upper side of the culture tank (1), and the adjustment component (2) includes an air pump (21), and the outlet end of the air pump (21) is connected to an air pipe (25), and the outer end of the air pipe (25) is inserted into the culture tank (1), a cross bar (3) is provided inside the culture tank (1), and a stirring component (4) is connected to the lower side of the cross bar (3), and a through hole (31) is provided inside the cross bar (3), and the stirring component (4) includes an insulating tube (41), and a heat-conducting sleeve (42) is provided inside the insulating tube (41), and an electric heating tube (43) is provided inside the heat-conducting sleeve (42).

2. A biological enzyme culture stirring device according to claim 1, characterized in that, A motor (5) and an electric control box (6) are installed on the outside of the bracket (12), and the output shaft end of the motor (5) is connected to the shaft rod at the outer end of the culture tank (1) through a gear set (51).

3. A biological enzyme culture stirring device according to claim 1, characterized in that: The air inlet end of the air pump (21) is connected to a dust cover (22), and a dust net (23) is provided inside the dust cover (22). One end of the air pipe (25) is connected to the air outlet end of the air pump (21), and a flow valve (24) is installed in the pipeline of the air pipe (25).

4. A biological enzyme culture stirring device according to claim 1, characterized in that: The cross bar (3) is installed along the length direction of the culture tank (1), and the two ends of the cross bar (3) are fixed to the inner walls on both sides of the culture tank (1).

5. A biological enzyme culture stirring device according to claim 1, characterized in that: A temperature sensor (13) and an oxygen content sensor (14) are installed on the outer wall of the culture tank (1), and the oxygen content sensor (14) is electrically connected to the air pump (21) through the electric control box (6).

6. A biological enzyme culture stirring device according to claim 1, characterized in that: The electric heating tube (43) is connected to the external temperature controller via a wire, and the temperature controller is electrically connected to the temperature sensor (13), and a connecting rod (44) is connected between the multiple groups of insulating tubes (41).

7. A biological enzyme culture stirring device according to claim 1, characterized in that: The inner wall of the culture tank (1) is provided with a flow hole (15) communicating with the outside, and the inner port of the flow hole (15) is provided with a waterproof breathable membrane (151), the outer port of the flow hole (15) is installed with a one-way valve (153), and a metal grid (152) is provided between the waterproof breathable membrane (151) and the one-way valve (153).

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