Escherichia coli pili purification device

Abstract

The utility model relates to the field of pili purification, and discloses an escherichia coli pili purification device which comprises a machine table, a supporting cylinder rotationally connected to the upper end of the machine table, a first motor arranged in the machine table and used for driving the supporting cylinder to rotate, through holes correspondingly formed in the two sides of the upper end of the supporting cylinder, and first gears rotationally connected to the interiors of the through holes in the two sides. One side of each first gear is fixedly connected with a swing arm, the other end of each swing arm is fixedly connected with a purification tank, a rotating hole is formed in the upper end of each purification tank, a connecting sleeve is rotatably connected in each rotating hole, one end, extending into each purification tank, of each connecting sleeve is fixedly connected with a filter screen cylinder, a filter membrane is arranged on the inner wall of each filter screen cylinder, and a sealing cover is arranged at the upper end of each connecting sleeve; a rotating mechanism for driving the connecting sleeve to rotate is arranged in the purifying tank, a sliding block is slidably connected in the supporting cylinder, and a double-sided rack meshed with the first gears on the two sides is fixedly connected to the upper end of the sliding block. Compared with the prior art, the device has the advantages that the purification and filtration speed is high, and the purification efficiency is greatly improved.

Description

Technical Field

[0001] The utility model relates to the technical field of pili purification, in particular to an Escherichia coli pili purification device. Background Art

[0002] Fimbriae are also a kind of protein, but their molecular weight is relatively large, generally exceeding 10 million Daltons (10,000KD), while the molecular weight of the impurity proteins mixed in the fimbriae is relatively small, generally only tens of thousands of Daltons. Therefore, appropriate ultrafiltration membranes can be used to separate and remove impurity proteins.

[0003] However, the existing E. coli fimbriae purification device has a relatively low filtration efficiency and requires a long waiting time, which greatly affects the experimental efficiency. Utility Model Content

[0004] (1) Technical problems solved

[0005] The technical problem to be solved by the utility model is that the existing purification device has a slow filtration speed, which greatly affects the purification efficiency.

[0006] (2) Technical solution

[0007] The transmission mechanism that this invention relates to is that this invention relates to a device for purifying bacterial colony, and this device comprises a gear train coupled to the gear train, and this gear train coupled to the gear train is coupled to the gear train, and this gear train coupled to the gear train is coupled to the gear train.

[0008] As an improvement, the rotating mechanism includes a gear ring fixedly connected to the outer wall of the connecting sleeve, the upper end of the purification tank is fixedly connected to a second motor, and the output end of the second motor is fixedly connected to a second gear meshing with the gear ring.

[0009] As an improvement, the reciprocating mechanism includes a turntable rotatably connected to the support cylinder, a third motor that drives the turntable to rotate is fixedly connected to one side of the support cylinder, adjustment holes that rotate with the turntable are provided on both sides of the support cylinder, an eccentric shaft is provided on one side of the turntable, an adjustment rod is rotatably connected to the eccentric shaft, and the upper end of the adjustment rod is hinged to the lower end of the slider.

[0010] As an improvement, a discharge hopper is fixedly connected to the lower end of the purification tank, a discharge pipe is fixedly connected to the bottom surface of the discharge hopper, and a switch valve is provided on the discharge pipe.

[0011] As an improvement, the upper end of the connecting sleeve is provided with an external thread, the sealing cover is threadedly connected to the upper end of the connecting sleeve, and the sealing cover is provided with an adjustment handle.

[0012] (3) Beneficial effects

[0013] The advantages of the utility model compared with the prior art are that: the connecting sleeve is driven by the rotating mechanism to drive the filter mesh cylinder to swing the internal Escherichia coli fimbriae, so that the purified fimbriae flow out of the filter mesh cylinder, and at the same time the reciprocating mechanism drives the swing arm to swing back and forth, the swing arm drives the purification tank to swing back and forth, the first motor drives the support cylinder to rotate, and the support cylinder then drives the purification tanks on both sides to rotate around the support cylinder, thereby greatly improving the filtration efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is an exploded view of an Escherichia coli fimbriae purification device of the utility model.

[0015] Figure 2 The utility model is a schematic structural diagram of an Escherichia coli fimbriae purification device.

[0016] Figure 3 This is a cross-sectional view of an Escherichia coli fimbriae purification device of the utility model.

[0017] Figure 4 The utility model is a schematic diagram of the reciprocating mechanism structure of an Escherichia coli fimbriae purification device.

[0018] As shown in the figure: 1. Machine table; 2. Support cylinder; 3. Through hole; 4. First gear; 5. Swing arm; 6. Purification tank; 7. Rotary hole; 8. Connecting sleeve; 9. Second motor; 10. Second gear; 11. Ring gear; 12. Slider; 13. Double-sided rack; 14. Turntable; 15. Third motor; 16. Adjusting rod; 17. Adjusting hole; 18. First motor; 19. Discharge pipe; 20. Switch valve; 21. Sealing cover; 22. Filter cylinder; 23. Discharge hopper; 24. Eccentric shaft. DETAILED DESCRIPTION

[0019] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0020] like Figures 1 to 4 As shown, an Escherichia coli fimbriae purification device includes a machine 1, the upper end of the machine 1 is rotatably connected to a support cylinder 2, a first motor 18 for driving the support cylinder 2 to rotate is provided in the machine 1, the upper end of the support cylinder 2 is provided with through holes 3 arranged correspondingly on both sides, and first gears 4 are rotatably connected in the through holes 3 on both sides, one side of the first gear 4 is fixedly connected to a swing arm 5, the other end of the swing arm 5 is fixedly connected to a purification tank 6, the lower end of the purification tank 6 is fixedly connected to a discharge hopper 23, the bottom surface of the discharge hopper 23 is fixedly connected to a discharge pipe 19, and a switch valve 20 is provided on the discharge pipe 19.

[0021] The upper end of the purification tank 6 is provided with a rotating hole 7, and a connecting sleeve 8 is rotatably connected in the rotating hole 7. The connecting sleeve 8 extends into the purification tank 6 and is fixedly connected to one end of a filter screen cylinder 22. The inner wall of the filter screen cylinder 22 is provided with a filter membrane. The upper end of the connecting sleeve 8 is provided with a sealing cover 21, and the upper end of the connecting sleeve 8 is provided with an external thread. The sealing cover 21 is threadedly connected to the upper end of the connecting sleeve 8, and an adjustment handle is provided on the sealing cover 21. A rotating mechanism for driving the connecting sleeve 8 to rotate is provided in the purification tank, and the rotating mechanism includes a gear ring 11 fixedly connected to the outer wall of the connecting sleeve 8. The upper end of the purification tank 6 is fixedly connected to a second motor 9, and the output end of the second motor 9 is fixedly connected to a second gear 10 meshing with the gear ring 11.

[0022] A slider 12 is slidably connected in the support cylinder 2, and a double-sided rack 13 meshing with the first gear 4 on both sides is fixedly connected to the upper end of the slider 12. A reciprocating mechanism for driving the slider 12 to slide is provided in the support cylinder 2, and the reciprocating mechanism includes a turntable 14 rotatably connected to the support cylinder 2, and a third motor 15 driving the turntable 14 to rotate is fixedly connected to one side of the support cylinder 2. Adjustment holes 17 that rotate with the turntable 14 are provided on both sides of the support cylinder 2, and an eccentric shaft 24 is provided on one side of the turntable 14. An adjusting rod 16 is rotatably connected to the eccentric shaft 24, and the upper end of the adjusting rod 16 is hinged to the lower end of the slider 12.

[0023] In specific use, the E. coli fimbriae liquid that needs to be purified is added to the filter screen cylinder 22. The inner wall of the filter screen cylinder 22 is provided with a filter membrane. The E. coli fimbriae liquid can be filtered and purified by the filter membrane. The sealing cover 21 is screwed on and the first motor 18 is started to rotate. The first motor 18 drives the support cylinder 2 to rotate. The support cylinder 2 drives the purification tank 6 to rotate through the swing arm 5. At the same time, the second motor 9 is started to drive the second gear 10 to rotate. The second gear 10 drives the gear ring 11 to drive the connecting sleeve 8 to rotate. The connecting sleeve 8 drives the filter screen cylinder 22 to rotate, so that the E. coli fimbriae liquid in the filter screen cylinder 22 is continuously thrown out, which greatly improves the filtration speed. At the same time, the third motor is started to rotate. The machine 15 drives the turntable 14 to rotate, and the turntable 14 drives the adjusting rod 16 to swing back and forth up and down through the eccentric shaft 24. The adjusting rod 16 pushes the slider 12 to slide back and forth on the inner wall of the support cylinder 2. The slider 12 drives the double-sided rack 13 to slide back and forth up and down, causing the first gears 4 on both sides to swing repeatedly. The first gears 4 on both sides drive the purification tanks 6 on both sides to swing back and forth up and down through the swing arms 5 on both sides, causing the E. coli fimbriae liquid in the filter cylinder 22 to shake in various ways, so that the purified E. coli fimbriae liquid flows out of the filter cylinder 22. When the purified E. coli fimbriae liquid needs to be recovered, turn the switch valve 20 to discharge the purified liquid from the discharge pipe 19.

[0024] The standard parts used in this utility model can all be purchased from the market, and special-shaped parts can be customized according to the description in the specification and the drawings. The specific connection methods of each part adopt conventional means such as mature bolts, rivets, welding, etc. in the existing technology. The machinery, parts and equipment all adopt conventional models in the existing technology, and the circuit connection adopts the conventional connection method in the existing technology, which will not be described in detail here.

[0025] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus.

[0026] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

[0027] The above description of the present invention and its embodiments is non-limiting. The drawings show only one embodiment of the present invention, and the actual structure is not limited thereto. In short, if a person skilled in the art is inspired by the above, and does not deviate from the purpose of the present invention, without creatively designing a structure and embodiment similar to the technical solution, they shall fall within the scope of protection of the present invention.

Claims

1. An Escherichia coli pili purification device, comprising a machine (1), wherein the upper end of the machine (1) is rotatably connected to a support cylinder (2), and a first motor (18) is provided in the machine (1) for driving the support cylinder (2) to rotate, characterized in that: The upper end of the support cylinder (2) is provided with through holes (3) arranged correspondingly on both sides, and the through holes (3) on both sides are rotatably connected to a first gear (4), one side of the first gear (4) is fixedly connected to a swing arm (5), and the other end of the swing arm (5) is fixedly connected to a purification tank (6), and the upper end of the purification tank (6) is provided with a rotating hole (7), and a connecting sleeve (8) is rotatably connected in the rotating hole (7), and the connecting sleeve (8) extends to the inside of the purification tank (6) and is fixedly connected to a filter at one end. A mesh cylinder (22), the inner wall of the filter mesh cylinder (22) is provided with a filter membrane, the upper end of the connecting sleeve (8) is provided with a sealing cover (21), the purification tank (6) is provided with a rotating mechanism for driving the connecting sleeve (8) to rotate, the support cylinder (2) is slidably connected with a slider (12), the upper end of the slider (12) is fixedly connected with a double-sided rack (13) meshing with the first gear (4) on both sides, and the support cylinder (2) is provided with a reciprocating mechanism for driving the slider (12) to slide.

2. The Escherichia coli fimbriae purification device according to claim 1, characterized in that: The rotating mechanism comprises a gear ring (11) fixedly connected to the outer wall of the connecting sleeve (8); a second motor (9) is fixedly connected to the upper end of the purification tank (6); and a second gear (10) meshing with the gear ring (11) is fixedly connected to the output end of the second motor (9).

3. The Escherichia coli fimbriae purification device according to claim 1, characterized in that: The reciprocating mechanism comprises a turntable (14) rotatably connected to the support cylinder (2); a third motor (15) for driving the turntable (14) to rotate is fixedly connected to one side of the support cylinder (2); adjustment holes (17) for rotating with the turntable (14) are provided on both sides of the support cylinder (2); an eccentric shaft (24) is provided on one side of the turntable (14); an adjustment rod (16) is rotatably connected to the eccentric shaft (24); and the upper end of the adjustment rod (16) is hinged to the lower end of the slider (12).

4. The Escherichia coli fimbriae purification device according to claim 1, characterized in that: The lower end of the purification tank (6) is fixedly connected to a discharge hopper (23), the bottom surface of the discharge hopper (23) is fixedly connected to a discharge pipe (19), and the discharge pipe (19) is provided with an on-off valve (20).

5. The E. coli fimbriae purification device according to claim 1, characterized in that: The upper end of the connecting sleeve (8) is provided with an external thread, the sealing cover (21) is threadedly connected to the upper end of the connecting sleeve (8), and the sealing cover (21) is provided with an adjustment handle.

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