Kit for DNA seamless cloning

Abstract

A kit for DNA seamless cloning comprises a kit body, a kit cover, a supporting rod, a supporting block, a lifting seat, a bearing plate, a reagent tube and a fixing plate, the upper portion of the kit cover is connected with a handle, the lower portion of the kit cover is connected with the kit body, the front side of the kit cover is provided with a spring bolt, the front side of the kit body is provided with a lock seat, the spring bolt is matched with the lock seat, the spring bolt is connected with the lock seat, and the rear side of the kit cover is provided with a connecting block. A connecting lug is arranged on the rear side of the kit and connected with a connecting block through a shaft rod, the shaft rod is sequentially connected with the connecting lug and the connecting block, the connecting block rotates on the shaft rod, a fixing plate is connected into the kit, vertical plates are arranged on the two sides of the fixing plate and connected with the kit through screws, and the screws are sequentially connected with the kit and the vertical plates.

Description

Technical Field

[0001] The utility model relates to the field of test kits, in particular to a test kit for seamless DNA cloning. Background Art

[0002] An existing patent (publication number: CN206385149U) proposes a DNA library construction kit comprising an outer shell and a partition. The outer shell houses an inner shell nested within it, with a gasket positioned on top. Sample tubes are arranged in a matrix on the gasket, and a low-temperature reservoir is movably mounted on the bottom of the inner shell corresponding to the sample tubes. A drying reservoir is also movably mounted on the bottom of the inner shell corresponding to the PCR tubes and the DNA sequencing tubes. The drying reservoir and the low-temperature reservoir are integrated. However, the "matrix arrangement of sample tubes on the gasket" in this device means that when a specific sample tube needs to be retrieved individually, multiple sample tubes must be retrieved and searched one by one, resulting in low efficiency and drawbacks. Summary of the Invention

[0003] In response to the above-mentioned deficiencies in the prior art, the utility model provides a reagent kit for seamless DNA cloning that is easy to transfer and carry, and has convenient reagent tube lifting and positioning control operations, thereby facilitating rapid differentiation and identification of reagent samples, thereby improving the efficiency of DNA seamless cloning reagent detection operations, and stably supporting the reagent tubes and enhancing their protection.

[0004] The purpose of this utility model is achieved through the following technical solutions:

[0005] A reagent kit for seamless DNA cloning comprises a reagent kit, a box cover, a support rod, a supporting block, a lifting seat, a supporting plate, a reagent tube, and a fixed plate. The upper portion of the box cover is connected to a handle, and the lower portion of the box cover is connected to the reagent kit. The front side of the box cover is provided with a locking tongue, and the front side of the reagent kit is provided with a locking seat. The locking tongue is adapted to the locking seat and is connected to the locking seat. The rear side of the box cover is provided with a connecting block, and the rear side of the reagent kit is provided with a connecting ear. The connecting ear is connected to the connecting block via a shaft rod, and the shaft rod is sequentially connected to the connecting ear and the connecting block, and the connecting block rotates on the shaft rod. The interior of the reagent kit is connected to a fixed plate, and vertical plates are provided on both sides of the fixed plate. The vertical plates are connected to the reagent kit via screws, and the screws are sequentially connected to the reagent kit and the vertical plates.

[0006] The vertical plate of the utility model has a sliding hole, which is adapted to the support block. The support block passes through the sliding hole and is connected to the lifting seat. The lifting seat has threaded grooves on both sides. The support block has a threaded rod, which is adapted to the threaded groove. The threaded rod is connected to the threaded groove through a thread. The support rod is sequentially connected to the vertical plate, the support rod, and the fixed plate. The lower end of the support rod has a lower circular plate, which fits the vertical plate.

[0007] The support rod of the utility model is divided into a positioning support rod and a limiting support rod. The upper end of the limiting support rod is connected to the upper circular plate, the upper circular plate is fitted into the fixed plate, the positioning support rod is connected to the support block through a thread, the upper end of the positioning support rod is connected to the motor, the motor is connected to the fixed plate, the fixed plate is connected to the cover plate, the cover plate is connected to the switch, and the switch is connected to the motor through wires.

[0008] The outer side of the lifting seat of the utility model is fitted with a vertical plate, the middle part of the lifting seat is provided with a central column, the central column has a supporting plate, the fixed plate has a central hole, the supporting plate is adapted to the central hole, the supporting plate passes through the central hole, the lower part of the supporting plate is provided with multiple upper supporting sleeves, and the upper supporting sleeves have placement holes.

[0009] The lifting seat of the utility model has multiple lower support sleeves, and the lower support sleeves have placement grooves. The placement grooves and placement holes are adapted to the reagent tubes. The reagent tubes pass through the placement holes, and the reagent tubes are connected to the placement grooves. The reagent tubes are connected to the inner side of the rubber pad, and the outer side of the rubber pad is connected to the lower support sleeves.

[0010] The reagent tube of the utility model is externally connected with a label, the upper part of the reagent tube is provided with a limiting card plate, the lower part of the limiting card plate is fitted with a supporting plate, the upper part of the limiting card plate is provided with a mounting sleeve, and the mounting sleeve is connected to the reagent cover through a thread. Beneficial effects

[0011] The utility model is used for connecting a DNA seamless cloning test kit with a box lid through an axis rod, which is convenient to open and close. The lock tongue and the lock seat are added to increase the security of the connection between the test kit and the box lid. A handle is provided on the upper part of the box lid for easy transfer and carrying of the test kit. The support rod increases the connection fulcrum between the support block, the vertical plate and the fixed plate, and the positioning support rod is positioned and connected to the support block through a thread. When the positioning support rod is driven by a switch to control the motor to rotate, the support block drives the lifting seat to perform longitudinal linear lifting displacement on the inner side of the vertical plate. When the lifting seat rises until its upper surface is in contact with the lower surface of the fixed plate, the support plate passes through the center hole and raises multiple groups of reagent tubes as a whole to their tube bodies to be exposed. The multiple groups of reagent tubes are evenly distributed axially and do not block each other. Labels are attached to the outside of the reagent tubes, and the labels can record reagent collection information, time, etc., which facilitates rapid identification of reagent samples. A specified sample reagent tube can be quickly taken out separately without taking out multiple groups of reagent tubes one by one for search, thereby improving the efficiency of DNA seamless cloning reagent detection operation.

[0012] When the switch controls the motor to drive the positioning support rod to rotate in the opposite direction, the support block drives the lifting seat to move down until its lower surface is in contact with the bottom surface of the reagent reagent box. At this time, the support plate passes through the center hole, and multiple groups of reagent tubes are moved down as a whole to be stored inside the vertical plate, thereby enhancing the protection of the reagent tubes. In addition, a threaded positioning connection is adopted between the positioning support rod and the support block, which can avoid the lifting seat from moving inside the vertical plate during the transfer of the DNA seamless cloning reagent reagent box, improve the stability of the reagent tube support, prevent the DNA seamless cloning reagent tubes from being disengaged and displaced, resulting in impact and breakage, and enhance the protection of the reagent tubes.

[0013] The upper support sleeve and the lower support sleeve of the utility model respectively limit and support the upper and lower ends of the reagent tube, so that it can be kept upright and placed vertically, thereby preventing the internal reagent from leaking due to tilting of the DNA seamless cloning reagent tube. The rubber pad added inside the lower support sleeve increases the friction resistance between the outer wall of the reagent tube and the contact surface of the lower support sleeve, thereby improving the placement stability of the reagent tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a schematic diagram of the connection structure of the fixed plate and the lifting seat described in the present utility model.

[0015] Figure 2 This is a structural schematic diagram of the lifting seat described in the utility model.

[0016] Figure 3 This is a structural schematic diagram of the lifting seat in the rising state of the utility model.

[0017] Figure 4 This is a schematic diagram of the fixed plate structure described in the utility model.

[0018] Figure 5 This is a cross-sectional view of the structure of a kit for seamless DNA cloning described in the present utility model.

[0019] Figure 6 This is a cross-sectional view of the structure of a kit for seamless DNA cloning described in the present utility model.

[0020] Figure 7 This is a top view of the connection structure of the test kit, fixing plate and supporting plate described in the present invention. DETAILED DESCRIPTION

[0021] The present invention will be described in further detail below with reference to the accompanying drawings and embodiments:

[0022] Example 1:

[0023] A reagent kit for seamless DNA cloning, comprising a reagent kit 01, a box cover 02, a support rod 08, a support block 11, a lifting seat 18, a supporting plate 25, a reagent tube 27, and a fixed plate 35. The upper portion of the box cover 02 is connected to a handle 32, and the lower portion of the box cover 02 is connected to the reagent kit 01. The front side of the box cover 02 has a lock tongue 06, and the front side of the reagent kit 01 has a lock seat 07. The lock tongue 06 is adapted to the lock seat 07, and the lock tongue 06 is connected to the lock seat 07. The rear side of the box cover 02 has a connecting block 04, and the rear side of the reagent kit 01 has a connecting ear 03. The connecting ear 03 is connected to the connecting block 04 through a shaft 05. The shaft 05 is sequentially connected to the connecting ear 03 and the connecting block 04, and the connecting block 04 rotates on the shaft 05. The interior of the reagent kit 01 is connected to the fixed plate 35, and both sides of the fixed plate 35 have vertical plates 33. The vertical plates 33 are connected to the reagent kit 01 through screws 34. The screws 34 are sequentially connected to the reagent kit 01 and the vertical plates 33. The reagent box 01 and the box cover 02 are connected by the shaft 05, which is convenient for opening and closing. The lock tongue 06 and the lock seat 07 are added to increase the security of the connection between the reagent box 01 and the box cover 02. A handle 32 is provided on the upper part of the box cover 02 to facilitate the transfer and carrying of the reagent box 01. The support rod 08 increases the connection fulcrum between the support block 11 and the vertical plate 33 and the fixed plate 35. At the same time, the positioning support rod 081 is positioned and connected with the support block 11 through a thread, so that when the switch 15 controls the motor 13 to drive the positioning support rod 081 to rotate, the support block 11 drives the lifting seat 18 to move longitudinally on the inner side of the vertical plate 33. Linear lifting displacement, when the lifting seat 18 rises to the point where its upper surface is in contact with the lower surface of the fixing plate 35, the supporting plate 25 passes through the center hole 26 and lifts the multiple groups of reagent tubes 27 as a whole to expose their tube bodies. The multiple groups of reagent tubes 27 are evenly distributed axially without blocking each other, and labels 29 are pasted on the outside of the reagent tubes 27. The labels 29 can record reagent collection information, time, etc., which is convenient for rapid identification of reagent samples. A specified sample reagent tube 27 can be quickly taken out separately without taking out multiple groups of reagent tubes 27 one by one to search, thereby improving the efficiency of DNA seamless cloning reagent detection operation.

[0024] Example 2:

[0025] The vertical plate 33 of the present invention has a sliding hole 12, which is adapted to the support block 11. The support block 11 passes through the sliding hole 12. The support block 11 is connected to the lifting seat 18. The lifting seat 18 has threaded grooves 10 on both sides. The support block 11 has a threaded rod 09, which is adapted to the threaded groove 10. The threaded rod 09 is connected to the threaded groove 10 through a thread. The support rod 08 is sequentially connected to the vertical plate 33, the support rod 08, and the fixed plate 35. The lower end of the support rod 08 has a lower circular plate 16, and the lower circular plate 16 is in contact with the vertical plate 33.

[0026] Example 3:

[0027] The support rod 08 of the utility model is divided into a positioning support rod 081 and a limiting support rod 082. The upper end of the limiting support rod 082 is connected to the upper circular plate 17, the upper circular plate 17 is attached to the fixing plate 35, the positioning support rod 081 is connected to the support block 11 through a thread, the upper end of the positioning support rod 081 is connected to the motor 13, the motor 13 is connected to the fixing plate 35, the fixing plate 35 is connected to the cover plate 14, the cover plate 14 is connected to the switch 15, the switch 15 is connected to the motor 13 through an electric wire, when the switch 15 controls the motor 13 to drive the positioning support rod 081 to rotate in the opposite direction, the support block 11 is rotated accordingly The lifting seat 18 is driven to move down until its lower surface is in contact with the bottom surface of the reagent reagent 01. At this time, the supporting plate 25 passes through the center hole 26, and the multiple groups of reagent tubes 27 are moved down as a whole to be stored inside the vertical plate 33, thereby enhancing the protection of the reagent tubes 27. In addition, a threaded positioning connection is adopted between the positioning support rod 081 and the support block 11, which can avoid the lifting seat 18 from moving inside the vertical plate 33 during the transfer of the reagent reagent 01, thereby improving the stability of the support for the reagent tube 27, preventing the reagent tube 27 from being disengaged and displaced, resulting in impact and breakage, and strengthening the protection of the reagent tube 27.

[0028] Example 4:

[0029] The outer side of the lifting seat 18 of the utility model is fitted with the vertical plate 33, and the middle part of the lifting seat 18 has a central column 24, the central column 24 has a supporting plate 25, the fixed plate 35 has a central hole 26, the supporting plate 25 is adapted to the central hole 26, the supporting plate 25 passes through the central hole 26, and the lower part of the supporting plate 25 has multiple upper support sleeves 22, and the upper support sleeves 22 have placement holes 19.

[0030] Example 5:

[0031] The lifting seat 18 of the present invention has multiple lower support sleeves 23, and the lower support sleeve 23 has a placement groove 20. The placement groove 20 and the placement hole 19 are adapted to the reagent tube 27. The reagent tube 27 passes through the placement hole 19, and the reagent tube 27 is connected to the placement groove 20. The reagent tube 27 is connected to the inner side of the rubber pad 21, and the outer side of the rubber pad 21 is connected to the lower support sleeve 23. The upper support sleeve 22 and the lower support sleeve 23 respectively limit and support the upper and lower ends of the reagent tube 27 to keep it upright and placed vertically, avoiding the internal reagent leakage caused by the tilt of the reagent tube 27 used for seamless DNA cloning, and the friction resistance between the outer wall of the reagent tube 27 and the contact surface of the lower support sleeve 23 is increased by the rubber pad 21 added inside the lower support sleeve 23, thereby improving the placement stability of the reagent tube 27.

[0032] Example 6:

[0033] The reagent tube 27 of the present invention is externally connected to a label 29, and the upper portion of the reagent tube 27 is provided with a limiting card plate 30, the lower portion of the limiting card plate 30 is fitted with the supporting plate 25, and the upper portion of the limiting card plate 30 is provided with a mounting sleeve 31, which is connected to the reagent cover 28 through a threaded connection.

[0034] Example 7:

[0035] Installation steps: First, assemble the driving structure of the lifting seat 18, first place the lifting seat 18 on the lower part of the fixed plate 35, and the outer surface of the lifting seat 18 fits with the inner surface of the vertical plate 33, the thread groove 10 corresponds to the position of the sliding hole 12, the supporting plate 25 passes through the center hole 26, and then the support block 11 passes through the sliding hole 12, and the threaded rod 09 is embedded in the thread groove 10, and is fixedly connected to the lifting seat 18 through the thread, then the support rod 08 passes through the vertical plate 33, the support block 11, and the fixed plate 35 in sequence until the upper surface of the lower circular plate 16 fits with the lower surface of the vertical plate 33, and the positioning support rod 081 is positioned and connected to the support block 11 through the thread, then the upper circular plate 17 is placed on the upper part of the fixed plate 35, and is fixedly connected to the upper end of the limiting support rod 082, The lower surface of the upper circular plate 17 is fitted with the fixed plate 35, and then the motor 13 is installed and fixed on the upper part of the fixed plate 35, and the motor 13 is driven and connected to the positioning support rod 081. Finally, the cover plate 14 is placed on the upper part of the motor 13 and fixedly connected to the fixed plate 35, and the switch 15 and the motor 13 are controlled and connected by wires. After the assembly of the lifting seat 18 driving structure is completed, the fixed plate 35 is placed in the reagent box 01, and the outer surface of the vertical plate 33 is fitted with the inner wall of the reagent box 01 and fixedly connected by screws 34. The lower surface of the lifting seat 18 is fitted with the bottom surface of the reagent box 01. Finally, the box cover 02 is placed on the upper part of the reagent box 01 and linked to it through the shaft 05, and the positions of the lock tongue 06 and the lock seat 07 are corresponding and connected to complete the installation.

[0036] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. It should be pointed out that ordinary technicians in this technical field can make several improvements and modifications without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A kit for seamless DNA cloning, characterized in that : It includes a reagent box (01), a box cover (02), a support rod (08), a support block (11), a lifting seat (18), a supporting plate (25), a reagent tube (27), and a fixing plate (35). The upper part of the box cover (02) is connected to the handle (32), and the lower part of the box cover (02) is connected to the reagent box (01). The front side of the box cover (02) has a lock tongue (06), and the front side of the reagent box (01) has a lock seat (07). The lock tongue (06) is adapted to the lock seat (07), and the lock tongue (06) is connected to the lock seat (07). The rear side of the box cover (02) has a connecting block (04), and the rear side of the reagent box (01) has a connecting ear (03). The connecting ear (03) is connected to the connecting block (04) through the shaft (05). The shaft (05) is sequentially connected to the connecting ear (03) and the connecting block (04). The connecting block (04) rotates on the shaft (05). The interior of the reagent box (01) is connected The fixing plate (35) is connected to the fixing plate (35). Both sides of the fixing plate (35) have vertical plates (33). The vertical plates (33) are connected to the reagent box (01) by screws (34). The screws (34) are connected to the reagent box (01) and the vertical plates (33) in sequence. The vertical plates (33) have a sliding hole (12). The sliding hole (12) is adapted to the support block (11). The support block (11) passes through the sliding hole (12). The support block (11) is connected to the lifting seat (18). Both sides of the lifting seat (18) have threaded grooves (10). The support block (11) has a threaded rod (09). The threaded rod (09) is adapted to the threaded groove (10). The threaded rod (09) is connected to the threaded groove (10) by threads. The support rod (08) is connected to the vertical plate (33), the support rod (08) and the fixing plate (35) in sequence. The lower end of the support rod (08) has a lower circular plate (16). The lower circular plate (16) is attached to the vertical plate (33).

2. A kit for seamless DNA cloning according to claim 1, characterized in that : The support rod (08) is divided into a positioning support rod (081) and a limiting support rod (082), the upper end of the limiting support rod (082) is connected to the upper circular plate (17), the upper circular plate (17) is fitted with the fixed plate (35), the positioning support rod (081) is connected to the support block (11) through a thread, the upper end of the positioning support rod (081) is connected to the motor (13), the motor (13) is connected to the fixed plate (35), the fixed plate (35) is connected to the cover plate (14), the cover plate (14) is connected to the switch (15), and the switch (15) is connected to the motor (13) through an electric wire.

3. A kit for seamless DNA cloning according to claim 2, characterized in that : The outer side of the lifting seat (18) is fitted with the vertical plate (33), the middle part of the lifting seat (18) has a central column (24), the central column (24) has a supporting plate (25), the fixed plate (35) has a central hole (26), the supporting plate (25) is adapted to the central hole (26), the supporting plate (25) passes through the central hole (26), the lower part of the supporting plate (25) has a plurality of upper support sleeves (22), and the upper support sleeves (22) have a placement hole (19).

4. A kit for seamless DNA cloning according to claim 3, characterized in that : The lifting seat (18) has a plurality of lower support sleeves (23), the lower support sleeve (23) has a placement groove (20), the placement groove (20) and the placement hole (19) are adapted to the reagent tube (27), the reagent tube (27) passes through the placement hole (19), the reagent tube (27) is connected to the placement groove (20), the reagent tube (27) is connected to the inner side of the rubber pad (21), and the outer side of the rubber pad (21) is connected to the lower support sleeve (23).

5. A kit for seamless DNA cloning according to claim 1, characterized in that The reagent tube (27) is externally connected to the label (29), the upper portion of the reagent tube (27) is provided with a limit card (30), the lower portion of the limit card (30) is fitted with the support plate (25), the upper portion of the limit card (30) is provided with a mounting sleeve (31), and the mounting sleeve (31) is connected to the reagent cover (28) through a thread.

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