Straw convenient for cell harvesting and passage
By designing a swingable L-shaped plastic straw and rubber sleeve blade, the problem of cell scraping and transfer in the existing technology is solved, the efficient discreteness and transfer of cell colonies is achieved, and the flexibility and precision of the operation are improved.
Patent Information
- Application Number
- CN202422260579.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-14
- Publication Date
- 2025-09-09
- Estimated Expiration
- 2034-09-14
AI Technical Summary
Existing L-shaped plastic straws make it difficult to simultaneously achieve effective discrete cell colonies and convenient cell transfer during cell scraping and transfer.
An L-shaped plastic straw was designed. A rotating device allowed the straw to swing inside the spherical shell. Combined with a rubber sleeve and a blade, it was used to scrape and absorb cell colonies. A scale was set on the outer wall of the straw to control the cell amount.
It achieves effective discreteness and convenient transfer of cell colonies, avoids cell damage, and improves operational flexibility and precision.
Smart Images
Figure CN223316679U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell passage scraping, and more particularly to a pipette which is convenient for cell harvesting and passage. Background Art
[0002] Cells are the fundamental structural and functional units of living organisms. In today's rapidly developing life sciences, major scientific advances such as animal cloning, stem cells, transgenic mammary bioreactors, and artificial tissues and organs are all based on the platform established by cell engineering. Cell engineering is a comprehensive biotechnology practice in which humans, following a specific design, manipulate cells, tissues, organs, and individuals through experimental manipulation at the cellular, subcellular, or tissue level to create superior products and varieties. Currently, the main technical areas of cell engineering include cell culture, cell fusion, cell splitting, chromosome manipulation, and gene transfer. Cell engineering has permeated many aspects of human life, including medications for treating diseases, vaccines for preventing diseases, cosmetics for beauty, and even food. Cell culture is the platform and foundation for the industrial application of cell engineering. Cell-engineered products can only be produced by cultivating large numbers of cells for production and research. Among the many cultured cells, embryonic stem cells (ESCs), derived from early human embryos, have fueled the rapid development and application of human regenerative medicine. Under appropriate induction and culture conditions, human embryonic stem cells (hESCs) can differentiate into all the cell types of the human body, including sperm and eggs. Therefore, they are a valuable source of cells for human tissue engineering, providing a large number of seed cells for the reconstruction and transplantation of human cells, tissues, and organs. HESCs are a representative cell population, including some cancer cells used in cancer drug efficacy studies. During cell culture, scraping is required to reduce cell clumps from large to small, detaching them from the culture vessel, and then seeding them onto new culture vessels to achieve cell passage. If digested using traditional trypsinization, hESCs, once digested into single cells, will not survive.
[0003] Existing cell scrapers use their tube walls to gradually cut large cell colonies into smaller cell colonies during the scraping process, thereby achieving the purpose of discrete cell colonies; however, the L-shaped shape is not convenient for transferring cells, so there is an urgent need for an L-shaped plastic straw that can both discrete cell colonies and facilitate cell transfer. Utility Model Content
[0004] The utility model aims to provide a straw that is convenient for cell harvesting and passage. The L-shaped plastic straw can control the swing angle of the straw, which can not only use the outer wall of the straw to discrete cell colonies, but also facilitate the operator to press the suction head to absorb and transfer cells.
[0005] The above technical objectives of the present invention are achieved through the following technical solutions: a pipette for facilitating cell harvesting and passage, comprising a pipette tip, a conical tube, and a wide-diameter tube, wherein the top and bottom of the wide-diameter tube are connected to the pipette tip and the conical tube, respectively; a rotating device is provided at the bottom of the conical tube; a pipette is provided at the end of the rotating device away from the conical tube;
[0006] The rotating device includes a spherical shell and a rotating ball shell; the top of the spherical shell is connected to the tapered tube, and the large-diameter tube, tapered tube and spherical shell are integrally formed; the outer wall of the rotating ball shell is rotatably connected to the inner wall of the spherical shell, the top and bottom of the rotating ball shell are both open, and the rotating ball shell is connected to the straw at the opening away from the tapered tube; the rotating ball shell and the straw are integrally formed, and the bottom of the spherical shell is provided with an opening for the straw to swing so that the straw can swing at a certain angle; the straw, spherical shell, rotating ball shell, tapered tube, large-diameter tube and the interior of the suction head are all connected.
[0007] By adopting the above technical solution, the rotating spherical shell is connected to the pipette, and the rotating spherical shell can swing at a certain angle within the spherical shell, so that the pipette can swing to a nearly horizontal position, which is convenient for the operator to perform discrete cell operations. In addition, it can be swung to a vertical position, which is convenient for the operator to suck cells into conical tubes and large-diameter tubes, thereby transferring cells.
[0008] The utility model is further configured as follows: a rubber sleeve is sleeved on the outer side wall of the straw, and a plurality of blades are equidistantly provided on the outer side wall of the rubber sleeve along an arc direction.
[0009] By adopting the above technical solution, the rubber sleeve is detachably connected to the straw, and multiple blades are installed on the outer wall of the rubber sleeve, so that cell colonies can be scraped off the culture dish; when the cell colonies need to be discrete, the rubber sleeve is removed and the outer wall of the straw is used for discreteness, thereby improving the applicability of the L-shaped plastic straw.
[0010] The utility model is further configured as follows: outer side walls of the tapered tube and the thick-diameter tube are provided with scales.
[0011] By adopting the above technical solution, the operator can control the amount of transferred cells according to the scale.
[0012] In summary, the present invention has the following beneficial effects: the rotating spherical shell is connected to the straw, and the rotating spherical shell can swing at a certain angle within the spherical shell, so that the straw can be swung to a nearly horizontal position, which is convenient for the operator to perform discrete cell operations. In addition, it can be swung to a vertical position, which is convenient for the operator to suck cells into conical tubes and large-diameter tubes, thereby transferring cells. BRIEF DESCRIPTION OF THE DRAWINGS
[0013] Figure 1 This is a half-section diagram of a pipette for facilitating cell harvesting and passage in an embodiment of the present utility model;
[0014] Figure 2 yes Figure 1 Cross-section at AA in the middle.
[0015] In the figure: 1. Suction tip; 2. Large diameter tube; 3. Scale; 4. Conical tube; 5. Spherical shell; 6. Straw; 7. Rubber sleeve; 8. Blade; 9. Rotating spherical shell. DETAILED DESCRIPTION
[0016] The following is combined with Figure 1 and attached Figure 2 The utility model is described in further detail.
[0017] Example: A pipette for facilitating cell harvesting and passage, such as Figure 1 and Figure 2 As shown, it includes a pipette tip 1, a tapered tube 4 and a large diameter tube 2. The top and bottom of the large diameter tube 2 are connected to the pipette tip 1 and the tapered tube 4 respectively. A rotating device is fixedly installed on the bottom of the tapered tube 4. The end of the rotating device away from the tapered tube 4 is connected to a pipette 6.
[0018] The rotating device includes a spherical shell 5 and a rotating spherical shell 9; the top of the spherical shell 5 is connected to the tapered tube 4, and the large-diameter tube 2, the tapered tube 4 and the spherical shell 5 are integrally formed; the outer wall of the rotating spherical shell 9 is rotatably connected to the inner wall of the spherical shell 5, and the top and bottom of the rotating spherical shell 9 are both open, and the rotating spherical shell 9 is connected to the straw 6 at the opening away from the tapered tube 4; and the rotating spherical shell 9 is integrally formed with the straw 6, and the bottom of the spherical shell 5 is provided with an opening for the straw 6 to swing so that the straw 6 can swing at a certain angle; the straw 6, the spherical shell 5, the rotating spherical shell 9, the tapered tube 4, the large-diameter tube 2 and the interior of the suction head 1 are all connected.
[0019] In this embodiment, when the operator needs to separate cells, the pipette 6 is swung to the maximum bending position, so that the larger cell colonies can be divided into smaller cell colonies by scraping the side walls of the pipette 6 without damaging the cells; when the operator transfers the cells to a new culture vessel, the pipette 6 can be rotated to the same vertical plane as the conical tube 4, which makes it easier for the operator to suck the cells into the conical tube 4 by pressing the pipette tip 1. If it is bent, it is not easy to suck the cell fluid into the thick-diameter tube 2 to the maximum extent due to the problem of resistance along the way.
[0020] The outer wall of the straw 6 is sleeved with a rubber sleeve 7 , and the outer wall of the rubber sleeve 7 is fixedly mounted with a plurality of blades 8 equidistantly along the arc direction, preferably 4 blades 8 , and the angle between two adjacent blades 8 is 90°.
[0021] In this embodiment, the rubber sleeve 7 is detachably connected to the straw 6, and a certain length of the rubber sleeve 7 is free of the blade 8, which facilitates the operator's installation or removal operations and prevents the blade 8 from scratching the operator's hands. The rubber sleeve 7 is sleeved on the straw 6, so that the cell colonies are scraped off the culture dish by the blade 8.
[0022] The outer side walls of the tapered tube 4 and the large diameter tube 2 are engraved with scales 3 .
[0023] In this embodiment, the operator can control the amount of transferred cells according to scale 3.
[0024] Working principle: The rotating spherical shell 9 is connected to the pipette 6, and the rotating spherical shell 9 can swing at a certain angle in the spherical shell 5, so that the pipette 6 can swing to a nearly horizontal position, which is convenient for the operator to perform discrete cell operations. In addition, it can be swung to a vertical position, which is convenient for the operator to suck cells into the conical tube 4 and the large-diameter tube 2, thereby transferring the cells.
[0025] This specific embodiment is merely an explanation of the present invention and is not a limitation of the present invention. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed. However, as long as they are within the scope of the claims of the present invention, they are protected by patent law.
Claims
1. A pipette for facilitating cell harvesting and passage, comprising a pipette tip (1), a conical tube (4), and a thick-diameter tube (2), wherein the top and bottom of the thick-diameter tube (2) are connected to the pipette tip (1) and the conical tube (4), respectively, and wherein: A rotating device is provided at the bottom of the conical tube (4); a suction pipe (6) is provided at the end of the rotating device away from the conical tube (4); The rotating device comprises a spherical shell (5) and a rotating spherical shell (9); the top of the spherical shell (5) is connected to the tapered tube (4), and the thick-diameter tube (2), the tapered tube (4) and the spherical shell (5) are integrally formed; the outer wall of the rotating spherical shell (9) is rotatably connected to the inner wall of the spherical shell (5), the top and bottom of the rotating spherical shell (9) are both open, and the rotating spherical shell (9) is connected to the straw (6) away from the opening of the tapered tube (4); the rotating spherical shell (9) and the straw (6) are integrally formed, and the bottom of the spherical shell (5) is provided with the straw (6), and the swingable opening enables the straw (6) to swing at a certain angle; the straw (6), the spherical shell (5), the rotating spherical shell (9), the tapered tube (4), the thick-diameter tube (2) and the suction head (1) are all connected.
2. A pipette for facilitating cell harvesting and passage according to claim 1, characterized in that: The outer side wall of the suction tube (6) is sleeved with a rubber sleeve (7), and the outer side wall of the rubber sleeve (7) is provided with a plurality of blades (8) at equal intervals along an arc direction.
3. The pipette for facilitating cell harvesting and passage according to claim 2, wherein: The outer side walls of the tapered tube (4) and the thick-diameter tube (2) are provided with scales (3).