Device for efficiently transferring cells
By designing a cell transfer device that includes a peristaltic pump, a shaker drive mechanism, and a semiconductor cooling module, the problems of low cell transfer efficiency, large errors, and temperature influence were solved, achieving efficient, quantitative, and stable cell transfer, and ensuring cell viability and experimental accuracy.
Patent Information
- Application Number
- CN202520237253.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-14
- Publication Date
- 2025-12-16
- Estimated Expiration
- 2035-02-14
AI Technical Summary
The problems of low efficiency, large operational errors, temperature changes affecting viability, and cell precipitation during cell transfer have not been effectively solved.
A device comprising a peristaltic pump, a shaking incubator drive mechanism, and a semiconductor refrigeration module is designed. The flow direction and volume of the peristaltic pump are adjusted by a display control module, the shaking incubator drive mechanism prevents cell precipitation, and the semiconductor refrigeration module controls the temperature to maintain cell viability.
This method achieves efficient, quantitative, and stable cell transfer, reduces the physical exertion of operators, and ensures cell viability and experimental accuracy.
Smart Images
Figure CN223674629U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to cell transfer technology, specifically relates to a device for cell high -efficient transfer. BACKGROUND
[0002] Cells are the smallest unit of life basic metabolism, the research of cell is a way for human to understand life science, the culture, amplification, passage and transfer of cell are the experimental steps that can not be bypassed in the process of cell production and scientific research, but the above process involves batch cell transfer, the volume of cell solution is large, the number of transfer containers is more, and the physical fitness of the operator is required.
[0003] Under normal circumstances, cell transfer is slow by natural gravity pouring or hand pressing on the fine bag, and long time can cause muscle fatigue of the operator, influence the subsequent operation precision, and the cell activity is reduced due to temperature change in the transfer process; human senses are not accurate, and the volume of each transfer cannot be the same, which influences the subsequent experimental precision; when the volume of the cell container is large, the operator cannot resuspend the cell solution by manual shaking, a large amount of cell sediment is caused, and the subsequent experiment is influenced. UTILITY MODEL CONTENT
[0004] The utility model solves the technical problem of providing a device that can solve the problems of low cell transfer efficiency, large personnel operation error, temperature change affecting activity and cell sedimentation in the process of cell culture, amplification, passage and the like.
[0005] To solve the above technical problems, the technical solution of the device for cell high -efficient transfer is:
[0006] Including the casing, one side in the casing is equipped with peristaltic pump, the other side in the casing is equipped with shaking table transmission mechanism;The shaking table transmission mechanism top is equipped with shaking table tray;The bottom of the shaking table tray is equipped with semiconductor refrigeration module;The casing peristaltic pump top is equipped with display control module;The bottom of the casing is equipped with detachable battery pack;The display control module is connected with detachable battery pack, semiconductor refrigeration module and shaking table transmission mechanism respectively;The top of the semiconductor refrigeration module is placed with cell culture container;The cell culture container is connected with liquid adding pipe;The liquid adding pipe is connected with peristaltic pump.
[0007] The detachable battery pack is lithium ion battery pack.
[0008] The side of the casing is equipped with the charging port for charging the detachable battery pack.
[0009] The left and right sides of the shaking table tray are connected with limiting devices; the limiting devices comprise two adjusting mechanisms symmetrically arranged front and back and a pressing plate vertically and slidably connected with the two adjusting mechanisms; the adjusting mechanism comprises a first connecting rod hingedly connected with the shaking table tray and a second connecting rod hingedly connected with the first connecting rod; the pressing plate is connected with vertical screw connecting rods corresponding to the second connecting rods of the two adjusting mechanisms at the front and back ends; the vertical screw connecting rods are vertically and slidably connected with the second connecting rods, and the vertical screw connecting rods are connected with control mechanisms for controlling the vertical screw connecting rods to move up and down; the cell culture container is connected with abutting plates corresponding to the pressing plates of the two limiting devices at the two sides; the pressing plate is pressed above the abutting plate; the first connecting rod and the second connecting rod are hingedly connected through a hinge shaft; the second connecting rod is hingedly connected with an adjusting plate at the middle part; the adjusting plate is provided with an arc-shaped adjusting groove coaxial with the hinge shaft; the arc-shaped adjusting groove is connected with a locking nut; the bottom end of the locking nut is threadedly connected with the first connecting rod through the arc-shaped adjusting groove.
[0010] The control mechanism is an upper nut and a lower nut; the upper nut and the lower nut are threadedly connected with the vertical screw connecting rods, and the upper nut and the lower nut are respectively located at the upper and lower sides of the second connecting rod.
[0011] The technical effects achieved by the device for efficient cell transfer include the following: the device for efficient cell transfer is designed with a semiconductor refrigeration module, which is used to control the temperature of cell suspension in the cell transfer process to maximize the cell survival rate, the refrigeration module uses semiconductor refrigeration, and the refrigeration temperature can be adjusted through the display control module; to prevent cell deposition at the bottom of the cell culture container during the cell transfer process, the device is designed with a shaking table transmission mechanism, the shaking frequency can be adjusted through the display control module during the transfer process, and cell deposition is effectively prevented; to efficiently transfer the cell solution, the device is internally provided with a peristaltic pump, the peristaltic pump is controlled through the display control module, and the liquid flow direction and the transfer volume (the liquid volume can be converted through the pipe diameter and the flow rate) are adjusted through the peristaltic pump, and the operation error is relatively small. BRIEF DESCRIPTION OF DRAWINGS
[0012] The utility model will be further explained in detail in connection with the drawings and specific embodiments:
[0013] Figure 1 is a structural schematic view of the device for efficient cell transfer of the utility model;
[0014] Figure 2 is Figure 1 the enlarged view of A of
[0015] Figure 3 is a partial top view of the device for efficient cell transfer of the utility model;
[0016] Figure 4 is Figure 3 a B part enlarged view of. DETAILED DESCRIPTION
[0017] The utility model will be further explained in detail below in combination with the drawings.
[0018] Referring to Figures 1 to 4 .
[0019] A device for efficient cell transfer, comprising a casing 1, a peristaltic pump 2 is installed on one side in the casing 1, a shaking table transmission mechanism 3 is installed on the other side in the casing 1; a shaking table tray 4 is installed above the shaking table transmission mechanism 3, a semiconductor refrigeration module 5 is installed at the bottom in the shaking table tray 4; a display control module 9 is installed above the peristaltic pump 2 of the casing 1, a detachable battery pack 6 is installed at the bottom of the casing 1, specifically, the detachable battery pack 6 is a lithium ion battery pack, a charging port 10 for charging the detachable battery pack 6 is arranged on the side of the casing 1, which is convenient for charging the detachable battery pack 6; the display control module 9 is connected with the detachable battery pack 6, the semiconductor refrigeration module 5 and the shaking table transmission mechanism 3 respectively; a cell culture container 7 is placed above the semiconductor refrigeration module 5, a liquid adding pipe 8 is connected to the cell culture container 7, and the liquid adding pipe 8 is connected with the peristaltic pump 2.
[0020] The utility model designs semiconductor refrigeration module 5, through semiconductor refrigeration module 5 for cell transfer process control cell suspension temperature to keep cell activity rate to maximum, this refrigeration module uses semiconductor refrigeration, can pass through display control module 9 to adjust refrigeration temperature;To prevent cell deposition in the cell culture container 7 bottom in the cell transfer process, the device also designs the shaking table transmission mechanism 3, can pass through display control module 9 to adjust the shaking frequency in the transfer process, effectively prevent cell deposition;For efficient cell solution transfer, the device is built-in peristaltic pump 2, through display control module 9 to control peristaltic pump 2 and pass through peristaltic pump 2 to adjust liquid flow direction and transfer volume (through pipe diameter and flow rate can convert liquid volume), and its operation error is relatively small.
[0021] The utility model discloses a cell transfer device, including the cell culture container 7, the peristaltic pump 2, the display control module 9 and the shaking bed tray 4, the cell culture container 7 is connected with the peristaltic pump 2, the display control module 9 is connected with the peristaltic pump 2, and the shaking bed tray 4 is connected with the cell culture container 7.
[0022] The utility model discloses the function needed in the process of multiple cell transfer is fused together, and the cell transfer work is high -efficient automation through shaking, refrigeration and quantitative transfer, and it is more beneficial to cell subsequent experiment state and quality stable line, the special bidirectional transfer mode avoids the applicability reduction caused by the single working mode of device, and the built-in energy is convenient for moving and experimental scene conversion, the special design concept can realize multiple container cell transfer by increasing the device quantity directly, so as to improve batch cell transfer efficiency and guarantee cell survival rate, the use of the device reduces the physical loss of operator, and can be used as the supporting factor of subsequent fine experiment operation, the quantitative transfer can avoid the operation difference problem caused by artificial transfer, and guarantees the uniformity and stability of cell transfer work.
[0023] Specifically, the utility model shaking bed tray 4 inside and left and right sides are connected with limiting device, limiting device includes two adjusting mechanism of symmetrical setting before and after and the pressing plate 11 of vertical sliding connection on two adjusting mechanism, adjusting mechanism includes with the first connecting rod 12 of hinged shaking bed tray 4 and with the second connecting rod 13 of hinged first connecting rod 12, and the pressing plate 11 before and after both ends are connected with the vertical screw connection rod 14 corresponding with the second connecting rod 13 of two adjusting mechanism, and vertical screw connection rod 14 is connected with the control mechanism for controlling the up-and-down movement of vertical screw connection rod 14, and the specific control mechanism is upper nut 15 and lower nut 16, and upper nut 15 and lower nut 16 are all screw connection on vertical screw connection rod 14, and upper nut 15 and lower nut 16 are located on the upper and lower sides of second connecting rod 13 respectively.
[0024] The cell culture container 7 is connected with the abutting plates 21 corresponding to the pressing plates 11 of the two limiting devices on both sides respectively, and the pressing plates 11 are pressed above the abutting plates 21; the first connecting rod 12 and the second connecting rod 13 are hinged through the hinge shaft 22, the middle part of the second connecting rod 13 is hinged with the adjusting plate 23, the arc-shaped adjusting groove 24 coaxial with the hinge shaft 22 is arranged on the adjusting plate 23, and the locking nut 25 is connected on the arc-shaped adjusting groove 24, and the bottom end of the locking nut 25 is screwed with the first connecting rod 12 through the arc-shaped adjusting groove 24.
[0025] The utility model can position the cell culture container 7, specifically, the cell culture container 7 is not easy to move relative to the rocking bed tray 4 through the limiting device inside left and right sides of the rocking bed tray 4, and more specifically, first, loosen the locking nut 25, now the first connecting rod 12 and the second connecting rod 13 can rotate relatively, make the pressing plate 11 of two limiting devices abut on both sides of the cell culture container 7 (reference Figure 1 、 3 ), then tighten the locking nut 25, make the first connecting rod 12 and the second connecting rod 13 can not rotate relatively, now the cell culture container 7 is positioned between the pressing plate 11 of two limiting devices, then rotate the upper nut 15 and the lower nut 16, adjust the height of the pressing plate 11, make the pressing plate 11 press to the above of the abutting plate 21, now the cell culture container 7 is positioned, and the cell culture container 7 is positioned between the pressing plate 11 and the semiconductor refrigeration module 5.
Claims
1. A device for efficient transfer of cells comprising a housing (1), characterized in that: The side of the casing (1) is provided with a peristaltic pump (2), the other side of the casing (1) is provided with a shaking table transmission mechanism (3); the top of the shaking table transmission mechanism (3) is provided with a shaking table tray (4); the bottom of the shaking table tray (4) is provided with a semiconductor refrigeration module (5); the top of the peristaltic pump (2) of the casing (1) is provided with a display control module (9); the bottom of the casing (1) is provided with a detachable battery pack (6); the display control module (9) is connected with the detachable battery pack (6), the semiconductor refrigeration module (5) and the shaking table transmission mechanism (3) respectively; the top of the semiconductor refrigeration module (5) is provided with a cell culture container (7); the cell culture container (7) is connected with a liquid adding pipe (8); the liquid adding pipe (8) is connected with the peristaltic pump (2).
2. The device for efficient cell transfer according to claim 1, wherein: The detachable battery pack (6) is a lithium ion battery pack.
3. The device for efficient cell transfer of claim 1, wherein: The side of the casing (1) is provided with a charging port (10) for charging the detachable battery pack (6).
4. The device for efficient cell transfer of claim 1, wherein: The left and right sides of the shaking table tray (4) are connected with limiting devices; the limiting devices include two adjusting mechanisms symmetrically arranged front and back and a pressing plate (11) vertically and slidably connected to the two adjusting mechanisms; the adjusting mechanism includes a first connecting rod (12) hinged to the shaking table tray (4) and a second connecting rod (13) hinged to the first connecting rod (12); the pressing plate (11) is connected with vertical screw connecting rods (14) corresponding to the second connecting rods (13) of the two adjusting mechanisms at the front and rear ends respectively; the vertical screw connecting rods (14) are vertically and slidably connected with the second connecting rods (13), and the vertical screw connecting rods (14) are connected with control mechanisms for controlling the vertical screw connecting rods (14) to move up and down; the cell culture container (7) is connected with abutting plates (21) corresponding to the pressing plates (11) of the two limiting devices on the two sides respectively; the pressing plate (11) is pressed above the abutting plate (21); the first connecting rod (12) and the second connecting rod (13) are hinged through a hinge shaft (22); the second connecting rod (13) is hinged with an adjusting plate (23) in the middle; the adjusting plate (23) is provided with an arc-shaped adjusting groove (24) coaxial with the hinge shaft (22); the arc-shaped adjusting groove (24) is connected with a locking nut (25); the bottom end of the locking nut (25) passes through the arc-shaped adjusting groove (24) and is threadedly connected with the first connecting rod (12).
5. The device for efficient cell transfer of claim 4, wherein: The control mechanism is an upper nut (15) and a lower nut (16); the upper nut (15) and the lower nut (16) are threadedly connected to the vertical screw connecting rod (14), and the upper nut (15) and the lower nut (16) are located on the upper and lower sides of the second connecting rod (13) respectively.